• The aim of the present study was to identify whether P. gingivalis heat‑shock protein 60 (HSP60) induced the dysfunction of human umbilical vein endothelial cells (HUVECs) in vitro. (spandidos-publications.com)
  • role in endothelial dysfunction. (crcm-marseille.fr)
  • Protein tyrosine phosphatase 1B (PTP1B) impairs nitric oxide (NO) production and induces endothelial dysfunction in various diseases, including diabetes, septic shock and heart failure. (crcm-marseille.fr)
  • We evaluated the link between PTP1B, ERS and endothelial dysfunction in mice. (crcm-marseille.fr)
  • Induction of ERS (Tunicamycin) in vivo in mice or ex vivo in mouse arteries led to severe arterial endothelial dysfunction (i.e. reduced flow-dependent, NO mediated dilatation in isolated small mesenteric arteries), and this was prevented by the PTP1B inhibitor trodusquemine and absent in PTP1B-/- mice. (crcm-marseille.fr)
  • SARS-CoV-2 infection primarily affects the pulmonary system, but accumulating evidence suggests that it also affects the pan-vasculature in the extrapulmonary systems by directly (via virus infection) or indirectly (via cytokine storm), causing endothelial dysfunction (endotheliitis, endothelialitis and endotheliopathy) and multi-organ injury. (nature.com)
  • The purpose of this review is to provide a latest summary of biomarkers associated with endothelial cell activation in COVID-19 and offer mechanistic insights into the molecular basis of endothelial activation/dysfunction in macro- and micro-vasculature of COVID-19 patients. (nature.com)
  • We envisage further development of cellular models and suitable animal models mimicking endothelial dysfunction aspect of COVID-19 being able to accelerate the discovery of new drugs targeting endothelial dysfunction in pan-vasculature from COVID-19 patients. (nature.com)
  • A number of viral species, such as dengue, ebola and cytomegalovirus can infect endothelial cells (ECs) and cause endothelial dysfunction [ 5 ]. (nature.com)
  • Taken together, the concerted actions of above factors lead to dysfunctional status of the vascular endothelium (endothelial dysfunction) (Fig. 1 ) [ 14 ]. (nature.com)
  • Fig. 1: SARS-CoV-2 induced endothelial dysfunction. (nature.com)
  • SARS-CoV-2 infection alters the balance of endothelial protective molecules and endothelial damaging molecules, leading to endothelial dysfunction. (nature.com)
  • BMP4 stimulates production of reactive oxygen species (ROS) and causes endothelial cell dysfunction. (houstonmethodist.org)
  • More specifically, proinflammatory adipokines from visceral adipose tissue may initiate the development of insulin resistance, microvascular dysfunction, and endothelial dysfunction. (hindawi.com)
  • Insulin resistance and endothelial dysfunction have both been implicated in the pathogenesis of hypertension [ 3 - 7 ] and atherosclerosis [ 8 , 9 ]. (hindawi.com)
  • and endothelial dysfunction measured with the increase in forearm blood flow from resting baseline to maximal vasodilation (FBF % increase). (hindawi.com)
  • The study was designed to evaluate the role of inflammation markers in the development of insulin resistance, microvascular dysfunction, and endothelial dysfunction and eventually the progression to (pre)diabetes and (pre)hypertension. (hindawi.com)
  • Hence, endothelial dysfunction, a predictor of several cardiovascular diseases (CVDs), is caused by imbalance between vasodilating and vasoconstricting agents, including NO, endothelium-derived hyperpolarizing factor, prostacyclin, or vasoconstrictive factors such as thromboxane (TXA 2 ) and endothelin-1 (ET-1) [ 8 ]. (hindawi.com)
  • Endothelial dysfunction (ED) and low-grade inflammation (LGI) have a role in the development of CVD. (maastrichtuniversity.nl)
  • Conditioned media from CBox treated macrophage induced greater endothelial dysfunction (monolayer permeability and inhibition of wound healing). (cdc.gov)
  • Tanner MJ, Wang J, Ying R, Suboc TB, et al: Dynamin-related protein 1 mediates low glucose-induced endothelial dysfunction in human arterioles. (msdmanuals.com)
  • Suboc TM, Dharmashankar K, Wang J, et al: Moderate obesity and endothelial dysfunction in humans: Influence of gender and systemic inflammation. (msdmanuals.com)
  • UCN-01, a protein kinase C (PKC) inhibitor, induces growth arrest and apoptosis in cancer cells and was recently introduced in a phase I clinical trial. (karger.com)
  • The expression of HMGB1, c-Jun N-terminal kinase (JNK), extracellular-signal-regulated kinase (ERK), and p38 mitogen-activated protein kinase (p38 MAPK) was assessed with immunofluorescence and western blot analysis. (molvis.org)
  • APC inhibits ET-induced TNF-α production in vitro in human monocytes by inhibiting activation of NFkB and AP-1 by inhibiting degradation of IkB and mitogen-activated protein kinase pathways, respectively. (eurekaselect.com)
  • Phosphorylation of hormone receptors by protein kinase C (PKC) may be involved in the regulation of receptor recycling. (elsevierpure.com)
  • Tunicamycin strongly increased the interactions of PTP1B with GRP78 and the activated forms of protein kinase RNA-like endoplasmic reticulum kinase (PERK) and IRE1α (proximity Ligation Assay). (crcm-marseille.fr)
  • Characterization of protein kinase C beta isoform's action on retinoblastoma protein phosphorylation, vascular endothelial growth factor-induced endothelial cell proliferation, and retinal neovascularization. (bvsalud.org)
  • Activation of protein kinase C (PKC) enhances the angiogenic process and is involved in the signaling of vascular endothelial growth factor ( VEGF ). (bvsalud.org)
  • Polyphenols are known to induce vasodilatory function via activation of the redox-sensitive phosphatidylinositol-3 (PI3)/protein kinase B (Akt) pathway. (nih.gov)
  • It was hypothesized that black currant fruit extracts would cause activation of endothelial nitric oxide synthase (eNOS) through activation of redox-sensitive PI3 kinase/Akt signaling pathway. (nih.gov)
  • Lung damage was apparent in animals receiving Pseu-Spike, revealed by thickening of the alveolar septa and increased infiltration of mononuclear cells (Figure [A]). AMPK (AMP-activated protein kinase) phosphorylates ACE2 Ser-680, MDM2 (murine double minute 2) ubiquitinates ACE2 Lys-788, and crosstalk between AMPK and MDM2 determines the ACE2 level. (cov19longhaulfoundation.org)
  • The tyrosine kinase receptors Tyro3, Axl and Mer (TAMs) and their ligands protein S and Gas6 are involved in the uptake of PS-exposing apoptotic cells in macrophages and dendritic cells. (lu.se)
  • In this study, we explore the role of HMGB1 in advanced glycation end products (AGEs)-induced vascular endothelial growth factor A (VEGF-A) production in rat retinal ganglion cell line 5 (RGC-5) cells. (molvis.org)
  • The VEGF-A protein and mRNA levels in conditioned medium of RGC-5 cells incubated with AGE-modified BSA (AGE-BSA) were examined with real-time PCR and enzyme-linked immunosorbent assay (ELISA), and BSA-treated cells were used as controls. (molvis.org)
  • Compared with the BSA controls, the RGC-5 cells incubated with AGE-BSA showed a dose- and time-dependent increase in VEGF-A mRNA and VEGF-A protein secretion in the supernatant, with the highest levels achieved at 24 h. (molvis.org)
  • Hsp90 associates with eNOS under inactive conditions, and upon the stimulation of endothelial cells with VEGF, estrogen, histamine, shear stress, and statins. (eurekaselect.com)
  • The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family and is active in angiogenesis, lymphangiogenesis, and endothelial cell growth. (nih.gov)
  • Activated SIRT1 signals hypoxia inducible factor (HIF)-2α, which transactivates vascular endothelial growth factor (VEGF) and erythropoietin. (molvis.org)
  • However, its role has not been evaluated in hypoxic choroidal endothelial cells (CECs), the precursor of VEGF-mediated choroidal angiogenesis. (molvis.org)
  • The mitogenic action of VEGF , a potent hypoxia -induced angiogenic factor , was increased by 2-fold in retinal endothelial cells by the overexpression of PKC beta 1 or beta 2 isoforms and inhibited significantly by the overexpression of a dominant-negative PKC beta 2 isoform but not by the expression of PKC alpha, delta , and zeta isoforms . (bvsalud.org)
  • Association of PKC beta 2 isoform with retinoblastoma protein was discovered in retinal endothelial cells , and PKC beta 2 isoform increased retinoblastoma phosphorylation under basal and VEGF -stimulated conditions. (bvsalud.org)
  • The potential functional consequences of PKC beta-induced retinoblastoma phosphorylation could include enhanced E2 promoter binding factor transcriptional activity and increased VEGF -induced endothelial cell proliferation. (bvsalud.org)
  • Purpose: Vascular Endothelial Growth Factor (VEGF) promotes angiogenesis in many different tumor types. (johnshopkins.edu)
  • This study assesses the prognostic value of VEGF protein levels in a cohort of patients with oral and oropharyngeal squamous cell carcinomas. (johnshopkins.edu)
  • VEGF protein levels were determined using immunohistochemistry. (johnshopkins.edu)
  • ABSTRACT We evaluated the prognostic value of serum endostatin and vascular endothelial growth factor (VEGF) for diagnosis of pre-eclampsia. (who.int)
  • RÉSUMÉ Nous avons évalué la valeur pronostique de l'endostatine et du facteur de croissance endothéliale vasculaire (VEGF) dans le sérum pour le diagnostic de la prééclampsie. (who.int)
  • We demonstrate that UCN-01, at concentrations lower than those necessary to inhibit cancer cell growth, inhibit proliferation of human endothelial cells in vitro. (karger.com)
  • Therefore, it is highly probable that tHGA may have anti-inflammatory effects on LPS-induced inflammation, Human Umbilical Vein Endothelial Cells (HUVECs) were used as an in vitro representative of endothelial cells. (upm.edu.my)
  • A lot of in vitro studies suggest that endothelial cell apoptosis might play a special role in the pathophysiology of micro- and macroangiopathy under certain conditions like diabetes or hyperlipidemia. (substack.com)
  • Pharmacological inhibitor studies confirmed an oxidant/MAPK/NFkb dependent pro-inflammatory response (gene and protein expression of CXCL9, 10, 11, TNF-a, IL-6) in mice and in in vitro exposed macrophages. (cdc.gov)
  • "Although no previous studies have examined this combination of amino acids on vascular function, we hypothesized that the active ingredients of the supplement would act synergistically to improve endothelial function by reducing oxidative stress and inflammation," ​ wrote the researchers. (nutraingredients-usa.com)
  • The title of my presentation that was given there is Eicosapentaenoic Acid or EPA modulates the expression or proteins linked to platelet activation and thrombosis in vascular endothelial cells during inflammation. (cardiologytalk.com)
  • So the objective of our study was to evaluate the effect of inflammation on the expression of proteins in human endothelial cells. (cardiologytalk.com)
  • Ample evidence shows that pro-atherosclerotic disturbed flow induces sustained activation of atherogenic genes in endothelial cells to promote their oxidation, inflammation, cell cycle progression and proliferation, whereas pulsatile shear stress tends to maintain endothelial cells in a quiescent and less proliferative state with a low level of oxidation and inflammation [16] , [17] . (plos.org)
  • 4) chronic tissue and organ damage characterized by thrombotic endothelialitis, endothelial inflammation, hyperactivated platelets, and fibrinaloid microclots. (cdc.gov)
  • C-reactive protein (CRP) is a marker of inflammation that is naturally present in the plasma at levels that may rise due to inflammatory processes, associated with a greater risk of cardiovascular events such as acute myocardial infarction. (bvsalud.org)
  • Endothelial protein C receptor (EPCR) also known as activated protein C receptor (APC receptor) is a protein that in humans is encoded by the PROCR gene. (wikipedia.org)
  • The protein encoded by this gene is a receptor for protein C that enhances its activation. (wikipedia.org)
  • The protein is also involved in Plasmodium falciparum malaria as subtypes of the Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family use EPCR of the host as a receptor. (wikipedia.org)
  • 2001). "Identification of the protein C/activated protein C binding sites on the endothelial cell protein C receptor. (wikidoc.org)
  • This protein regulates signal transducing molecules, which include members of the Srckinase family of non-receptor tyrosine kinases, serine/threonine kinases and transcription factors. (eurekaselect.com)
  • Qin R , Zhu H , Wang F , Song M , Lin P , Xing Y , Zhang W , Zhong M , Wang Z , . Platelet activation in diabetic mice models: the role of vascular endothelial cell-derived protein disulfide isomerase-mediated GP IIb/IIIa receptor activation. (aging-us.com)
  • Endothelial cells express both PKD1 (polycystin-1), an eleven transmembrane domain protein, and PKD2 (polycystin-2), a transient receptor potential (TRP) channel. (grantome.com)
  • We have studied the recycling and the phosphorylation state of the insulin growth factor (IGF) II/mannose 6-phosphate (Man-6-P) receptor in microvascular endothelial cells from rat adipose tissue. (elsevierpure.com)
  • A subclass of receptor-like protein tryosine phosphatases that contain a single cytosolic protein tyrosine phosphate domain and multiple extracellular fibronectin III-like domains. (online-medical-dictionary.org)
  • Proteins that do not bind the receptor are directed to lysosomal degradation. (nature.com)
  • Schlemm's canal cell isolates reacted with antibodies specific for CD44 (hyaluron receptor), CD54 (intercellular adhesion molecule-1, ICAM-1), tissue-type plasminogen activator, and TM-inducible glucocorticoid-responsive protein-myocilin (TIGR-MYOC). (nih.gov)
  • Mohandas A, Suboc TB, Wang J, et al: Mineralocorticoid exposure and receptor activity modulate microvascular endothelial function in African Americans with and without hypertension. (msdmanuals.com)
  • The purpose of this study was to investigate whether unfolded proteins accumulate in the corneal endothelium in FECD and if they are involved in triggering cell death. (cardiff.ac.uk)
  • Thus, PTP1B plays a central role in the regulation of ERS in the endothelium, and the endothelial protective effect of PTP1B inhibition appears likely due at least in part to reduction of endothelial ERS, notably by promoting PERK protective pathway. (crcm-marseille.fr)
  • Acutely, hemin sensitizes endothelial cells to oxidants but chronically protects the endothelium through the induction of ferritin. (umn.edu)
  • Given the Spike Protein's interaction with the Endothelium, especially via the disruption of Calcium homeostasis, triggers Endothelial cell Apoptosis. (substack.com)
  • Aim 3 will study the hypothesis that hypertension is associated with pathological alterations in PKD1/PKD2 channel signaling in endothelial cells that inhibits vasodilation mediated by these proteins. (grantome.com)
  • APC regulates the coagulation system by inactivating the activated form of factors V and VIII in the presence of protein S. Tumor necrosis factor-α(TNF-α) plays critical roles in the development of disseminated intravascular coagulation, acute respiratory distress syndrome and shock in sepsis by inducing endothelial cell damage through activation of neutrophils. (eurekaselect.com)
  • APC reduces the pulmonary endothelial cell injury and hypotension in rats administered endotoxin (ET) by inhibiting TNF-α production through inhibition of its transcription. (eurekaselect.com)
  • In endothelial cells, inhibition of NCX by either pharmacological compounds [91] or treatment with ROS causes prolonged mitochondrial Ca2+ elevation and, in consequence, also insufficient Ca2+ refilling of the ER during continuous stimulation, the latter of which has been generally neglected so far. (substack.com)
  • The chronic effects of PKCd inhibition using either rottlerin or the overexpression of a dominant negative PKCd mutant included the downregulation of eNOS gene expression that was manifested by a decrease in both eNOS promoter activity and protein expression after 24 h of treatment. (vectorbiolabs.com)
  • Widlansky ME, Puppula V, Suboc TM, et al: Impact of DPP-4 inhibition on acute and chronic endothelial function in humans with type 2 diabetes on background metformin therapy. (msdmanuals.com)
  • Eicosapentaenoic Acid: Modulated Platelet Activation and Thrombosis Proteins in Vascular Endothelial Cells During Inf. (cardiologytalk.com)
  • Vascular endothelial cells, as a monolayer in direct contact with the flowing blood, bear the most of the wall shear stresses and have important homeostatic functions in response to stress [16] . (plos.org)
  • 3 In the current study, we show that S protein alone can damage vascular endothelial cells (ECs) by downregulating ACE2 and consequently inhibiting mitochondrial function. (cov19longhaulfoundation.org)
  • As the lesions enlarge, the covering endothelial cells initially become stretched, and they eventually fall off. (medscape.com)
  • These observations strongly suggest that APC might be involved not only in regulation of the coagulation system, but in regulation of inflammatory responses by preventing endothelial cell injury. (eurekaselect.com)
  • Transport of NO and ROS by AQPs would be required for cell homeostasis to play a critical role in maintaining endothelial function. (hindawi.com)
  • Conclusions: Excessive synthesis of extracellular matrix proteins induced unfolded protein accumulation in FECD. (cardiff.ac.uk)
  • The Spike Protein alone causes an influx of extracellular Ca2+. (substack.com)
  • We found that an upstream calcium-dependent PKC isozyme PKC alpha that modulates the downstream ERK/NF-kappaB pathway through an influx of extracellular Ca2+ is induced by the spike protein of SARS-CoV. (substack.com)
  • D , Measurement of oxygen consumption rate (OCR, D , i and iii) and extracellular acidification rate (ECAR, D , ii and iv) in ECs infected with ACE2-D vs ACE2-L (10 MOI) for 48 h (n=3) or treated with IgG vs S1 protein (4 μg/mL) for 24 h (n=3). (cov19longhaulfoundation.org)
  • Malik M, Suboc TM, Tyagi S, et al: Lactobacillus plantarum 299v supplementation improves vascular endothelial function and reduces inflammatory biomarkers in men with stable coronary artery disease. (msdmanuals.com)
  • The levels of vascular endothelial (VE)‑cadherin, endothelial nitric oxide synthase (eNOS) and cysteinyl aspartate‑specific protease‑3 (caspase‑3) were measured using western blot analysis. (spandidos-publications.com)
  • "Although investigation of this mechanism was beyond the scope of this study, it is feasible that the arginine in the supplement improved endothelial-dependent vasodilation by providing additional substrate for nitric oxide synthesis," ​they added. (nutraingredients-usa.com)
  • Low PDCD4 level was associated with reduced proliferation but not apoptosis or phosphorylation of endothelial nitric oxide synthase caused by pulsatile shear stress to help maintain the homeostasis of endothelial cells. (plos.org)
  • In this study, we explore the roles of the delta isoform of PKC (PKCd) in the regulation of endothelial nitric oxide synthase (eNOS) activity in pulmonary arterial endothelial cells isolated from fetal lambs (FPAECs). (vectorbiolabs.com)
  • Furthermore, superoxide anions can modify endothelial function by reducing nitric oxide (NO) biosynthesis and bioavailability [ 7 ]. (hindawi.com)
  • Here we pursue an a priori hypothesis that genetic variation in the endothelial-based receptors of the thrombomodulin-protein C system (THBD and PROCR) may similarly be associated with early-onset ischemic stroke. (lu.se)
  • Hsp90 is well preserved and exhibits a chaperone role in the conformational maturation in the cellular stress response, and the nuclear hormone receptors and protein kinases. (eurekaselect.com)
  • This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-2 and VEGFR-3 receptors. (nih.gov)
  • These data suggest a role for PKC-mediated serine phosphorylation in the regulation of intracellular trafficking of receptors in the endothelial cells. (elsevierpure.com)
  • P. gingivalis HSP60 at a concentration of 10 ng/l significantly decreased the expression levels of VE‑cadherin and eNOS protein at 24 h stimulation, whereas no difference in these proteins was identified following a low dose of P. gingivalis HSP60 (1 ng/l). (spandidos-publications.com)
  • P. gingivalis HSP60 at 100 ng/l significantly downregulated the expression levels of VE‑cadherin and eNOS protein at 12 h in HUVECs. (spandidos-publications.com)
  • To test this hypothesis, human umbilical vein endothelial cells (HUVECs) were treated with different concentrations/times of black currant juice concentrates (Ben Gairn and Ben Hope) and the activation of Akt and eNOS was measured using immunoblotting. (nih.gov)
  • Furthermore, complementary increased and decreased phosphorylation of eNOS (endothelial NO synthase) Thr-494 and Ser-1176 indicated impaired eNOS activity. (cov19longhaulfoundation.org)
  • ETU significantly inhibited NO production, eNOS promoter activity, and eNOS protein levels. (vectorbiolabs.com)
  • Vascular endothelial cell (EC)-derived factors play an important role in endothelial-cardiomyocyte crosstalk and could save cardiomyocytes (CMs) from injury. (mdpi.com)
  • This issue is of particular relevance since changes in NO release could play an important role in endothelial function maintenance, in addition to regulating proliferation of smooth muscle cells, leukocyte adhesion, platelet aggregation, angiogenesis, thrombosis, vascular tone, and hemodynamics. (hindawi.com)
  • Regulatory mechanisms, physiological functions and pathological involvement during hypertension of PKD1 protein and PKD2 channels in endothelial cells are unclear. (grantome.com)
  • Aim 1 will test the hypothesis that endothelial-dependent physiological stimuli activate PKD1/PKD2 coupling in endothelial cells, leading to vasodilation. (grantome.com)
  • Under physiological circumstances, the resistance of the endothelial cell lining to interactions with platelets and coagulation factors prevents thrombosis. (medscape.com)
  • As bioactive compounds tend to interact with multiple proteins with various physiological effect, we aimed to identify other potential shared targets to understand the extent in which these chemicals could lead to GWI. (cdc.gov)
  • During both normal development and pathology, the formation of new vessels and the regression of preexisting ones are dependent on the balance between endothelial cell proliferation and endothelial cell apoptosis. (substack.com)
  • P-selectin, a granule membrane protein of platelets and endothelial cells, follows the regulated secretory pathway in AtT-20 cells. (rupress.org)
  • P-selectin (PADGEM, GMP-140, CD62) is a transmembrane protein specific to alpha granules of platelets and Weibel-Palade bodies of endotheial cells. (rupress.org)
  • The hemostatic system consists of platelets, coagulation factors, and the endothelial cells lining the blood vessels. (medscape.com)
  • Platelet activation allows binding of these proteins, which bridges adjacent platelets. (medscape.com)
  • Why I find this fascinating are the circumstances of apoptosis in endothelial cells. (substack.com)
  • Endothelial mitochondria are essential to the functional integrity of the endothelial cell as they integrate a wide range of cellular processes including Ca2+ handling, redox signaling and apoptosis, all of which are closely interrelated. (substack.com)
  • Miembro de factores de transcripción que contienen dedos de cinc que pertenece a la familia de los FACTORES DE TRANSCRIPCIÓN DE TIPO KRUPPEL, implicado en la regulación de diversos procesos celulares tales como el crecimiento, la proliferación, la diferenciación y la APOPTOSIS celulares. (bvsalud.org)
  • This impairment of cellular Ca2+ signaling could conceivably affect endothelial function and, in the worst case, both excess mitochondrial Ca2+ and ER stress could trigger apoptotic pathways. (substack.com)
  • As circulating PMPs-levels were not altered in Gas6-/- mice compared to Gas6+/+ mice, we hypothesize that the Gas6-mediated uptake is not a means to clear the bulk of circulating PMPs but may serve to phagocytose PMPs locally generated at sites of platelet activation and as a way to affect endothelial responses. (lu.se)
  • EPCR protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. It belongs to the MHC class I/CD1 family of proteins, that is characterized by having a deep groove, that in other proteins in the family (but not in EPCR) is usually used for antigen binding. (wikipedia.org)
  • The bound lipid in EPCR is usually phosphatidylcholine, but it may be phosphatidylethanolamine, and it contributes to protein C binding, though probably not through direct contact Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy. (wikipedia.org)
  • This review focuses on emerging topics encompassing the functional involvement of aquaporin channel proteins (AQPs) and membrane transport systems, also allowing permeation of NO and hydrogen peroxide, a major ROS, in oxidative stress physiology and pathophysiology. (hindawi.com)
  • Mitochondria have a critical function in triggering this enzymatic cascade of self-destruction by releasing a set of proteins into the cytosol. (substack.com)
  • However, the dynamic changes of miRNA distribution in mitochondria and their mechanisms in reactive oxygen species (ROS)-induced endothelial injury remain unclear. (figshare.com)
  • Therefore, miRNA levels in whole cells and mitochondria of H 2 O 2 -treated endothelial cells were analyzed by small RNA sequencing in the present study. (figshare.com)
  • To unravel the mechanism of miR-301a-3p distribution in mitochondria, RNA pull-down followed by label-free quantitative proteomic analysis was performed, and RNA-binding protein Musashi RNA binding protein 2 (MSI2) was found to specifically bind to miR-301a-3p. (figshare.com)
  • H 2 O 2 significantly downregulated MSI2 expression in whole endothelial cells, promoted the distribution of MSI2 in cytosol and decreased its distribution in the mitochondria. (figshare.com)
  • Thus, MSI2 might be responsible for the distribution of miR-301a-3p between cytosol and mitochondria in endothelial cells. (figshare.com)
  • To investigate whether the mechanism of targeting of P-selectin to granules is specific for megakaryocytes and endothelial cells and/or dependent on von Willebrand factor, a soluble adhesive protein that is stored in the same granules, we have expressed the cDNA for P-selectin in AtT-20 cells. (rupress.org)
  • Fasting levels and 12 h postprandial responses of markers of ED (soluble intercellular adhesion molecule 1 (sICAM), soluble vascular cell adhesion molecule 1 (sVCAM), soluble endothelial selectin and von Willebrand factor) and markers of LGI (serum amyloid A, C-reactive protein and sICAM) were evaluated before and after intervention. (maastrichtuniversity.nl)
  • We hypothesized that macrophage uptake of CBox NPs and secretion of soluble factors mediate enhanced endothelial cell damage abilities of CBox. (cdc.gov)
  • Certain studies show that P. gingivalis lipopolysaccharide (LPS) could induce the expression of intercellular adhesion molecule 1 and vascular cell adhesion molecule 1 in human umbilical vein endothelial cells (HUVECs) ( 13 , 14 ), which significantly enhances trans-endothelial migration of inflammatory cells. (spandidos-publications.com)
  • Application of unidirectional pulsatile shear stress to human umbilical vein endothelial cells (HUVECs) decreased PDCD4 protein but not mRNA level. (plos.org)
  • The results showed that H 2 O 2 significantly reduced the relative mitochondrial distribution of dozens of miRNAs in human umbilical vein endothelial cells (HUVECs). (figshare.com)
  • Under normal condition, the endothelial cells form a compact monolayer to line the blood vessel. (upm.edu.my)
  • Endothelial cells line the wall of all blood vessels and regulate a wide variety of functions, including contractility which controls systemic blood pressure. (grantome.com)
  • Glycation, the result of a protein or lipid molecule bonding with sugar molecules, is a consequence of the aging process. (molvis.org)
  • Endothelial cells are sentinels lining the innermost layer of blood vessel that gatekeep micro- and macro-vascular health by sensing pathogen/danger signals and secreting vasoactive molecules. (nature.com)
  • Endothelial cells regulate arterial contractility. (grantome.com)
  • We investigated whether atheroprotective unidirectional pulsatile shear stress affects the expression of PDCD4 in endothelial cells. (plos.org)
  • En face co-immunostaining of the mouse aortic arch revealed a low level of PDCD4 in endothelial cells undergoing pulsatile shear stress. (plos.org)
  • Glycocalyx Core Proteins Selectively Mediate Endothelial NOS activation and Cell Alignment in Response to Shear Stress. (ebonglab.org)
  • wp-content/themes/awp-enfold/blank.png 0 0 Academic Web Pages /wp-content/themes/awp-enfold/blank.png Academic Web Pages 2013-01-01 00:00:00 2019-07-09 19:25:47 Glycocalyx Core Proteins Selectively Mediate Endothelial NOS activation and Cell Alignment in Response to Shear Stress. (ebonglab.org)
  • Methods: Descemet's membranes with corneal endothelial cells (CECs) were obtained during keratoplasty, and expression of aggresomes, type 1 collagen, fibronectin, and agrin was evaluated. (cardiff.ac.uk)
  • The purpose of this study was to investigate the release of Endothelial microparticle (EMP)-associated protein disulfide isomerase (PDI) after endothelial cell injury induced in diabetes and the changes in platelet activation. (aging-us.com)
  • Vertebrate endothelial lipase: comparative studies of an ancient gene and protein in vertebrate evolution. (wakehealth.edu)
  • Disrupted protein interactions identify gene sets involved in risk for ASD. (springer.com)
  • Specifically, the PDCD4 protein combines directly with the mRNA coding region of the target gene ( MYB/c-MYB ) to block translation [2] . (plos.org)
  • High-mobility group box 1 protein (HMGB1) has been reported to be a potent proangiogenic factor induced by inflammatory stress. (molvis.org)
  • The expression of bone morphogenic protein 4 (BMP4), a new pro-inflammatory marker, is increased by disturbed flow in endothelial cells (ECs). (houstonmethodist.org)
  • Oxidized phospholipids are thought to market atherogenesis by rousing endothelial cells (ECs) to create inflammatory cytokines such as for example IL-8. (careersfromscience.org)
  • Periodontitis is a chronic infection capable of inducing an inflammatory response that increases the level of proteins during the acute phase, such as C-reactive protein, and considering that these proteins are independent risk factors for cardiovascular diseases, this could explain the connection between periodontal disease and heart disease that is, periodontal diseases increase the risk of cardiovascular diseases. (bvsalud.org)
  • However, the action of PDCD4 in endothelial cells is unclear. (plos.org)
  • We will investigate the novel hypothesis that PKD1 and PKD2 proteins regulate arterial contractility, investigate signaling mechanisms involved and test the concept that vasodilation mediated through these pathways is dysfunctional during hypertension. (grantome.com)
  • Using a wide variety of approaches and inducible, endothelial cell-specific knockout mice, we provide evidence that physical coupling of PKD1 to PKD2 in endothelial cells stimulates vasodilation. (grantome.com)
  • Aim 2 will investigate the mechanisms by which endothelial-dependent stimuli activate PKD1/PKD2 channels in endothelial cells to produce vasodilation. (grantome.com)
  • These results are clinically relevant because reduced endothelial-dependent vasodilation is a known risk factor for cardiovascular diseases. (nutraingredients-usa.com)
  • Macrophage-endothelial cell cross talk in ozone-oxidized carbon black nanoparticle exposure. (cdc.gov)
  • We display that several recognized modules are significantly enriched in genes for known pathways and confirm a module enriched for unfolded protein response (UPR) genes using siRNA and the UPR inducer tunicamycin. (careersfromscience.org)
  • Purpose: The unfolded protein response (UPR) is believed to play a role in the pathogenesis of Fuchs' endothelial corneal dystrophy (FECD). (cardiff.ac.uk)
  • Fuchs endothelial dystrophy (FED) is characterized by an asymmetrical, bilateral, slowly progressive edema of the cornea in elderly patients. (medscape.com)
  • the rate is accelerated in Fuchs endothelial dystrophy. (medscape.com)
  • Familial Fuchs endothelial dystrophy in a 65-year-old female. (medscape.com)
  • The left eye of a 75-year-old man showing fully developed Fuchs endothelial dystrophy. (medscape.com)
  • Fuchs endothelial dystrophy passes through 4 clinical stages. (medscape.com)
  • Dysfunctional endothelial cells are a hallmark of several cardiovascular diseases, but pathological mechanisms involved are poorly understood. (grantome.com)
  • The uptake of labeled MPs in the presence of protein S and Gas6 in human aortic endothelial cells (HAEC) and human umbilical vein endothelial cells (HUVEC) was monitored by flow cytometry, western blotting and confocal/electron microscopy. (lu.se)
  • Endothelial cell adaptation via heme-induced heme oxygenase and ferritin production might limit ultimate progression to pulmonary and other vascular leak syndromes. (umn.edu)
  • Antinuclear antithrombin, protein C, protein S or pres- antibodies were investigated with standard- ence of antiphospholipid antibodies, are ized enzyme-linked immunosorbent assay common in patients with retinal vein occlu- sions and may contribute to the etiology of (ELISA). (who.int)
  • We conclude that UCN-01, at clinically relevant concentrations, exerts an anti-neovascularization effect by blocking two important steps in vessel formation: (1) the response of cancer cells to hypoxia, and (2) endothelial cell proliferation. (karger.com)
  • Using the newly released and larger ASC dataset, we confirm these observations and take them in several new directions: (1) By defining a set of genes encoding these disrupted protein interactors in ASD subjects and another for their siblings, we evaluate their expression patterns in developing brain from fetal to early postnatal development and within general cell types of brain tissue. (springer.com)