• Scientists have breathed new life into the study of a protein with an outsized link to human cancers because of its dangerous mutations, using advanced research techniques to detect its hidden regions. (scienceblog.com)
  • We know that structural biology can provide unique insights into the mechanisms of those mutations and can stimulate the search for potential cures. (scienceblog.com)
  • The study revealed two "switch" regions - tellingly, both located near a protein loop where the most dangerous mutations occur - in the K-Ras structure that had not been visible before. (scienceblog.com)
  • Truncating RAD21 variants are shown above, and missense mutations and in-frame deletions are shown below the protein representation. (springer.com)
  • We closely collaborate with researchers within BIOCEV as well as with external groups on analysis of structural data, rational design of protein mutations, and design of ligands and inhibitors of studied proteins. (biocev.eu)
  • To investigate the emergence of compensatory mutations in the viral proteins during cell passaging, the consensus sequences of the entire polyprotein coding regions of the wild type JFH1 and three chimeric viruses were determined. (monash.edu)
  • Several mutations in the N terminus of the G-protein-coupled receptor rhodopsin disrupt NXS/T consensus sequences for N-linked glycosylation (located at N2 and N15) and cause sector retinitis pigmentosa in which the inferior retina preferentially degenerates. (jneurosci.org)
  • Mutations affecting glycosylation of the heptahelical G-protein-coupled receptor rhodopsin are associated with retinitis pigmentosa (RP), a disease characterized by progressive degeneration of photoreceptors. (jneurosci.org)
  • The unique synonymous mutations detected in the E and Non-structural 2a genes of Usutu-BONN strains may suggest an adaptive evolution. (cdc.gov)
  • Insights into Disease-Associated Mutations in the Human Proteome through Protein Structural Analysis Structure, 23(7):1362-9. (lu.se)
  • Pires D, Rodrigues C, Ascher D, mCSM-membrane: predicting the effects of mutations on transmembrane proteins, Nucleic Acids Res;48(W1):W147-W153. (lu.se)
  • Garcia-Recio A, Gómez-Tamayo J, Reina I, Campillo M, Cordomí A, Olivella M, TMSNP: a web server to predict pathogenesis of missense mutations in the transmembrane region of membrane proteins, NAR Genom Bioinform. (lu.se)
  • Ge F, Zhu Y, Xu J, Muhammad A, Song J, Yu D, MutTMPredictor: Robust and accurate cascade XGBoost classifier for prediction of mutations in transmembrane proteins, Comput Struct Biotechnol J. 2021 Nov 19;19:6400-6416. (lu.se)
  • SCOP was created in 1994 in the Centre for Protein Engineering and the Laboratory of Molecular Biology. (wikipedia.org)
  • It was maintained by Alexey G. Murzin and his colleagues in the Centre for Protein Engineering until its closure in 2010 and subsequently at the Laboratory of Molecular Biology in Cambridge, England. (wikipedia.org)
  • We would like to announce the following symposium taking place in Zürich, Switzerland this September 5th International NCCR Symposium on New Trends in Structural Biology September 15 + 16, 2006 Auditorium Maximum, ETH Zürich, Zürich, Switzerland Confirmed speakers to date: Nenad Ban ¦ Patrick Cramer ¦ Yoshinori Fujiyoshi ¦ Helmut Grubmüller ¦ Markus Grütter ¦ Masatsune Kainosho ¦ Karolin Luger ¦ Ray Stevens The online application slot will open on the symposium web site end of April. (bio.net)
  • NEW YORK (GenomeWeb) - Researchers from the European Molecular Biology Laboratory-European Bioinformatics Institute and the University of Oxford have devised a periodic table of protein complexes. (genomeweb.com)
  • He noted that while, in the past, much structural biology work has focused on homomeric complexes involving just one kind of protein, technological advances, particularly in the field of cryo-electron microscropy are enabling better characterization of large multiprotein complexes. (genomeweb.com)
  • The Cusack group uses X-ray crystallography and cryo electron-microscopy (cryoEM) to study the structural biology of protein-RNA complexes involved in RNA virus replication, innate immunity and cellular RNA metabolism. (embl.org)
  • Structural biology investigations of BCL-2 proteins have provided tremendous insights into our understanding of their structure-function relationships and models have been proposed to explain how the BCL-2 family members form a network of interactions to control apoptosis signaling. (elsevierpure.com)
  • The textbook Molecular Biology of the Cell (4th edition, 2002), from the NCBI Bookshelf, offers a detailed introduction to protein function . (medlineplus.gov)
  • Now a large and ever increasing number of genetic subtypes has been described, and major advances in molecular and cellular biology have clarified the understanding of the role of different proteins in the physiology of peripheral nerve conduction in health and in disease. (medscape.com)
  • The Ras family of proteins are enzymes that set in motion the growth, division and differentiation of many types of cells, and their genes have been identified as the most frequently mutated cancer-related genes in humans. (scienceblog.com)
  • Four of structural genes, p11.7, p15.1, p28.4, and p31.7 were identified by matrix assisted laser desorption/ionization time-of-flight mass spectroscopy. (umass.edu)
  • Cloning structural genes for Treponema pallidum immunogens and characterisation of recombinant treponemal surface protein, P2 (P2 star). (bmj.com)
  • Streamlining Regulon Identification in Bacteria Regulons are a group of genes that can be turned on or off by the same regulatory protein. (doe.gov)
  • The Structural Classification of Proteins (SCOP) database is a largely manual classification of protein structural domains based on similarities of their structures and amino acid sequences. (wikipedia.org)
  • A motivation for this classification is to determine the evolutionary relationship between proteins. (wikipedia.org)
  • Similar to CATH and Pfam databases, SCOP provides a classification of individual structural domains of proteins, rather than a classification of the entire proteins which may include a significant number of different domains. (wikipedia.org)
  • The new Structural Classification of Proteins version 2 (SCOP2) database was released at the beginning of 2020. (wikipedia.org)
  • The unit of classification of structure in SCOP is the protein domain. (wikipedia.org)
  • The table, which the researchers presented in a paper published this month in Science , provides a classification system that covers roughly 92 percent of known protein complex structures while also enabling predictions of new structures likely to be observed in the future. (genomeweb.com)
  • Standard NMR can follow a fast-acting protein but has trouble with a longer time scale of movement and interactions, and X-ray crystallography used to define protein structures does better with less movement and more time. (scienceblog.com)
  • It's more trying to get at the structural basis of the interactions," he added. (genomeweb.com)
  • As could be demonstrated, the oligomerization is based on two independent interactions mediated by different domains of the protein: a known interaction of the N-terminal discoidin domain with the C-terminal portion, termed head-to-tail interaction, and a dimerization of the LisH motif in muskelin that was so far neglected in the literature. (uni-wuerzburg.de)
  • when both interactions were disturbed, the protein was present as monomer. (uni-wuerzburg.de)
  • Cdk enzymatic activity is tightly controlled through cyclin interactions, posttranslational modifications, and binding of inhibitors such as the p27 tumor suppressor protein. (escholarship.org)
  • The research concentrates on understanding the structural and functional features of biologically relevant molecules and their interactions employing the tools of Structural Bioinformatics and Molecular Modeling. (biocev.eu)
  • Using this approach, the functional interactions of p7 and E2 with other viral proteins during HCV replication was investigated. (monash.edu)
  • In summary, this thesis demonstrates the importance of functional interactions between HCV proteins during virus morphogenesis and infectivity. (monash.edu)
  • Furthermore, we will discuss the structural details of the interactions between BCL-2 family members and the various structural paradigms that ultimately regulate the activation of apoptosis. (elsevierpure.com)
  • p7 is a highly hydrophobic, integral membrane protein and is classified in the viroporin family. (monash.edu)
  • BCL-2 proteins are a family of pro- and anti-apoptotic proteins that regulate a critical step in the mitochondrial apoptotic pathway, the permeabilization of the mitochondrial outer membrane. (elsevierpure.com)
  • We will discuss the structural insights for each structural domain of BCL-2 proteins that determine their function in the cytosol and the outer mitochondrial membrane. (elsevierpure.com)
  • N-linked glycosylation is the most prevalent posttranslational modification of plasma membrane and secretory proteins and participates in many important biological roles such as protein folding, intracellular targeting, immune response, cell adhesion, and protease resistance. (jneurosci.org)
  • Membrane protein datasets with a total of 2058 variants in F1. (lu.se)
  • Orioli T, Vihinen M, Benchmarking subcellular localization and variant tolerance predictors on membrane proteins, BMC Genomics;20(Suppl 8):547. (lu.se)
  • The cell counters RNA viruses with innate immune pattern-recognition receptors, such as the RNA helicase RIG-I, which recognise particular viral RNA structural motifs (e.g. 5′ triphosphate-dsRNA) as non-self, thus activating a signalling pathway leading to interferon production and establishment of the anti-viral state. (embl.org)
  • As a result, many viral proteins are multifunctional machines that do not directly obey the classic structure-function paradigm. (ox.ac.uk)
  • Often, such multifunctionality is rooted in intrinsic disorder that allows viral proteins to interact with various cellular factors and remain functional in the hostile environment of different cellular compartments. (ox.ac.uk)
  • EXPERT OPINION: Small sizes of viral genomes impose multifunctionality on their proteins, which is dependent on the excessive usage of intrinsic disorder. (ox.ac.uk)
  • The recently developed HCV cell culture system, based on the JFH1 strain of HCV, has provided an opportunity to study the role of the viral proteins in the complete HCV replication cycle in human hepatoma cells. (monash.edu)
  • How the viral proteins functionally interact during replication of HCV in cell culture is not completely understood. (monash.edu)
  • Since the protein plays a critical role in the virus life cycle, chemical compounds that block p7 function are potential candidates for anti-viral therapy. (monash.edu)
  • The viral proteins are then transferred onto nitrocellulose paper and reacted with the patient's serum. (cdc.gov)
  • Positive and negative control serum specimens are run simultaneously to allow identification of viral proteins. (cdc.gov)
  • However, in a recent study, the protein with a mobility of 160 kilodaltons (kd) present in commercially available Western blots and in viral lysate antigen preparations was identified as a multimer of the gp41 protein (10,11). (cdc.gov)
  • Although the overall sensitivity and specificity of the Western blot for detection of antibodies to the various viral proteins are high, there has been substantial debate regarding the interpretive criteria. (cdc.gov)
  • In this strain, 1 putative cleavage site of the viral polyprotein responsible for processing of structural proteins was changed. (cdc.gov)
  • Proteins with the same shapes but having little sequence or functional similarity are placed in different superfamilies, and are assumed to have only a very distant common ancestor. (wikipedia.org)
  • Proteins having the same shape and some similarity of sequence and/or function are placed in "families", and are assumed to have a closer common ancestor. (wikipedia.org)
  • The MICS program is developed based on a systematic study of the NMR chemical shift (and amino acid sequence) patterns observed for each type of structural motif by using a database of proteins of known structure and known NMR chemical shifts. (nih.gov)
  • Two transmembrane domains were predicted in p11.7, and a DNA binding motif was found in p31.7 by amino acid sequence analysis, therefore p11.7 and p31.7 were predicted to be envelope and capsid protein, respectively. (umass.edu)
  • Our work implies that the rich structural information extracted from fragment libraries can complement sequence-derived features to help protein structure prediction. (biomedcentral.com)
  • During the prediction, MICS will generate a single output file ' predMICS.tab ' to store the the normalized probability of finding each motif at any given position in the protein, respectively. (nih.gov)
  • Based on the genome sequencing results, an analysis of structural proteins and prediction of putative microRNAs of Hz-2V was performed. (umass.edu)
  • Fragment libraries play a key role in fragment-assembly based protein structure prediction, where protein fragments are assembled to form a complete three-dimensional structure. (biomedcentral.com)
  • To better leverage the valuable structural information for protein structure prediction, we extracted seven types of structural information from fragment libraries. (biomedcentral.com)
  • We broadened the usage of such structural information by transforming fragment libraries into protein-specific potentials for gradient-descent based protein folding and encoding fragment libraries as structural features for protein property prediction. (biomedcentral.com)
  • Although fragment libraries are extensively utilized in fragment assembly, the rich structural information has not yet been systematically analyzed and leveraged by other protein structure prediction approaches. (biomedcentral.com)
  • Designed proteins [58788] (44): Experimental structures of proteins with essentially non-natural sequences. (wikipedia.org)
  • Solution NMR structures of proteins VPA0419 from Vibrio parahaemolyticus and yiiS from Shigella flexneri provide structural coverage for protein domain family PFAM 04175. (rostlab.org)
  • However, viruses, being small in size, can only harbor a genome large enough to code for the minimal number of proteins required for the replication and maturation of the virions. (ox.ac.uk)
  • In this thesis, a chimeric JFH1 genome that encodes the p7 protein of genotype (GT) 1b strain J4 was generated. (monash.edu)
  • Table 1 lists the major structural proteins coded for by the HIV genome. (cdc.gov)
  • ABSTRACT This study evaluated peripheral eosinophil and serum eosinophilic cationic protein (s-ECP) levels as markers of asthma control. (who.int)
  • Neither did antibody to the recombinant 37,000 dalton protein react with any treponemal proteins purified by fibronectin affinity chromatography. (bmj.com)
  • The recombinant protein was not recognised by antiserum directed against a fibronectin binding treponemal adhesion that contained the same electrophoretic mobility. (bmj.com)
  • Structural changes were detected by transmission electron microscopy (TEM), and levels of the hepatic fibrosis-related proteins collagen I (Col-I), alpha-smooth muscle actin (α-SMA) and osteopontin (OPN) were measured by western blotting and enzyme-linked immunosorbent assay (ELISA). (biomedcentral.com)
  • Despite existing knowledge about K-Ras and its key functional relationships with molecules related to cell health, the protein has been deemed "undruggable" because its configuration - both in normal and mutated forms - hides sites in its structure that would be most promising as therapeutic targets. (scienceblog.com)
  • This study was undertaken to isolate the protein from jackfruit seeds and characterizing the physicochemical and functional properties of the isolated protein. (banglajol.info)
  • The functional properties such as solubility and gelling capacity and secondary structural elements of jackfruit seed protein isolate (JSPI) were studied. (banglajol.info)
  • Based on the observed structural and functional features, JSPI has prospects to be used as a supplementary ingredient in future food formulations. (banglajol.info)
  • Our structural work on influenza polymerase has opened up the area of structure-based drug design of novel anti-virals targeting multiple functional sites on the polymerase. (embl.org)
  • Structural and Functional Analysis of "non-smelly" Proteins" by Jing Yan, Jianlin Cheng et al. (usf.edu)
  • We assessed the abundance, structural coverage, and functional characteristics of the "non-smelly" proteins, i.e., proteins that do not contain cysteine residues (C-depleted) or cysteine and aromatic residues (CFYWH-depleted), across 817 proteomes from all domains of life. (usf.edu)
  • In addition, some recent works such as [ 19 , 20 ] adopted structural information in other bioinformatics fields and the considerable performance gains indicate the huge potential of protein structural information. (biomedcentral.com)
  • TY - JOUR T1 - Structural characterization of Escherichia coli ribosomal protein S8 and its binding site in 16S ribosomal RNA. (unboundmedicine.com)
  • To establish a basis for understanding the mode of operation of muskelin, the aim of this thesis was an in-depth biochemical and structural characterization of muskelin and its interaction with the GABA(A) receptor. (uni-wuerzburg.de)
  • The backbone is essential to understanding a protein's structural properties - from there, characterizing amino acid side chains "is relatively straightforward," Brüschweiler said. (scienceblog.com)
  • The chemical shifts, together with the PDB-extracted amino acid preference of the helix capping and beta-turn motifs, are then used as input data for training an artificial neural network algorithm, which outputs the statistical probability of finding each motif at any given position in the protein. (nih.gov)
  • Previously, we worked on aminoacyl-tRNA synthetases, which play an essential role in protein synthesis by charging specifically their cognate tRNA(s) with the correct amino acid and editing mischarged amino acids if necessary (Palencia et al . (embl.org)
  • Semi-automatically derived and hand-curated collection of proteins, which possess an amino acid that has been changed by a SNV and 3D atomic coordinates are available in the PDB. (lu.se)
  • The photoisomerization reaction of a fluorescent protein chromophore occurs on the ultrafast timescale. (nature.com)
  • Now, high-resolution pump-probe X-ray crystallography reveals complex sub-ångström, ultrafast motions and hydrogen-bonding rearrangements in the active site of a fluorescent protein. (nature.com)
  • As shown on the figure (so called Jablonski diagram), when a molecule (e.g. a fluorescent protein) in the electronic ground state absorbs a photon of suitable wavelength, it accesses the first electronically excited state. (ibs.fr)
  • For FPs, one often compares the MB to that of enhanced Green Fluorescent Protein (EGFP), which is about 30000 M -1 .cm -1 . (ibs.fr)
  • The structural basis for the photochromism in the fluorescent protein Dronpa is poorly understood, because the crystal structures of the bright state of the protein did not provide an answer to the mechanism of the photochromism, and structural determination of the dark state has been elusive. (psu.edu)
  • Creative Biolabs offers a variety of mRNA products capable of encoding many types of structural proteins, which can be applied to the study of biological mechanisms of structural proteins, the study of wound repair functions, the investigation of tumour-related regulatory mechanisms and the development of gene therapy drugs. (creative-biolabs.com)
  • With IVTScrip™ synthesis platform and professional mRNA design team, our structural protein mRNA products are all of high quality, high translation rate and low delivery cycle. (creative-biolabs.com)
  • Furthermore, we offer purification processes and mass spectrometry characterisation at all stages to ensure the purity and authenticity of our structural protein mRNA products. (creative-biolabs.com)
  • Myelinating Schwann cells form a myelin sheath around a single axon and express high levels of myelin-related proteins and messenger RNA (mRNA). (medscape.com)
  • Our next experiments indicated differences in FLG protein and in mRNA expression using AD-associated cytokines. (cdc.gov)
  • Treponemal proteins ranging in molecular weight from 37,000 daltons to 120,000 daltons were identified by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and immunoblotting of phage lysate proteins. (bmj.com)
  • The Western blot assay is a method in which individual proteins of an HIV-1 lysate are separated according to size by polyacrylamide gel electrophoresis. (cdc.gov)
  • Insulin-like growth factor-binding proteins (IGFBPs) control bioavailability, activity, and distribution of insulin-like growth factor (IGF)1 and -2 through high-affinity IGFBP/IGF complexes. (rcsb.org)
  • Marsh and his colleagues developed the table by analyzing the assembly and disassembly pathways for a number of protein complexes. (genomeweb.com)
  • Also key, he noted, was mass spec work by the lab of Oxford researcher and paper co-author Carol Robinson and her colleagues, who use mass spec to experimentally characterize the assembly and disassembly pathways of protein complexes. (genomeweb.com)
  • An important part of [the table] is based on the sort of limited types of closed symmetry groups that proteins can adopt when they form complexes," he said. (genomeweb.com)
  • The table covers 92.5 percent of observed homomer complexes and 91.7 percent of heteromers, the authors noted, and, of the remaining 8 percent, roughly half of these exceptions are due to "basically either crystallographic artifacts or mistakes people have made when assigning the biological unit in the Protein Data Bank," Marsh said. (genomeweb.com)
  • We also showed that the "non-smelly" proteins typically did not independently fold into monomeric structures, and instead, they fold by interacting with nucleic acids as constituents of the ribosome and nucleosome complexes. (usf.edu)
  • Finally, only sera of patients with primary syphilis possessed appreciable concentrations of antibody to recombinant P2 protein. (bmj.com)
  • Thus, the structure of thin and wavy junctions differed only in the extent of crystallization of MIP, a property that can explain why this protein can produce two different antibody-labeling patterns. (rupress.org)
  • A monoclonal antibody raised against this protein labeled these thicker junctions on the cytoplasmic surfaces of both apposing membranes. (rupress.org)
  • A primary objective in malaria vaccine design is the generation of high-quality antibody responses against the circumsporozoite protein of the malaria parasite, Plasmodium falciparum (PfCSP). (nature.com)
  • Solvent Accessibility of Residues Undergoing Pathogenic Variations in Humans: From Protein Structures to Protein Sequences, Front Mol Biosci. (lu.se)
  • Using these collections of structural information, Marsh and his colleagues used principles of protein complex symmetry to determine the range of possible protein complex structures. (genomeweb.com)
  • So it kind of recapitulates what is known about [protein] symmetry, but puts it in a new assembly-centric framework. (genomeweb.com)
  • The lipocalins are also part of a larger protein superfamily: the calycins, which includes the fatty acid binding proteins, avidins, a group of metalloproteinase inhibitors, and triabin. (aston.ac.uk)
  • They were shown to be involved in translation, transcription, nucleosome assembly, transmembrane transport, and protein folding functions, all of which are known to be associated with the intrinsic disorder. (usf.edu)
  • The structural dynamics that result from femtosecond optical excitation have contributions from vibrational and electronic processes and from reaction dynamics that involve the crossing through a conical intersection. (nature.com)
  • The creation and progression of the ultrafast structural dynamics strongly depends on optical and molecular parameters. (nature.com)
  • The team also established the complex structural dynamics behavior of the protein "backbone" that amplified additional features close to the switches. (scienceblog.com)
  • Careful phenotyping is essential in interpreting consequences of RAD21 variants, and protein modeling and dynamics can be helpful in determining pathogenicity. (springer.com)
  • The diversity of the photosynthesis proteins due to evolution upon adaptation and exploitability is intriguing for researchers from all fields of science to understand aspects of structural diversity, function and dynamics. (rug.nl)
  • In addition, Creative Biolabs also offers customized services for mRNAs that can encode specific structural proteins. (creative-biolabs.com)
  • For example, there are 284 folds in the "All alpha proteins" class. (wikipedia.org)
  • Design and identification of stable protein folds suitable for mutagenesis. (biocev.eu)
  • While not aimed primarily at proteomics research, the table could prove a useful resource for the field in that it provides structural information that can be added to protein-protein interaction experiments, noted Joseph Marsh, an EMBL researcher and author on the paper. (genomeweb.com)
  • What the SCOP authors mean by "domain" is suggested by their statement that small proteins and most medium-sized ones have just one domain, and by the observation that human hemoglobin, which has an α2β2 structure, is assigned two SCOP domains, one for the α and one for the β subunit. (wikipedia.org)
  • An important problem in protein folding is to understand the relationship between the structure of a denatured ensemble and its thermodynamics. (nih.gov)
  • After maturation, these three amino-acids form a para-HydroxyBenzylideneDimethylImidazolinone (p-HBDI) structure, responsible for the light absorption and emission properties of the protein. (ibs.fr)
  • The barrel structure produces a very tight packing of the interior of the protein, so that the chromophore and water molecules are largely fixed into place by hydrogen bonds, with limited (but not impossible) diffusion of ions or small molecules. (ibs.fr)
  • Those structural motifs are well known to play a key role in stabilizing protein structure and likely to be important in the protein folding process. (nih.gov)
  • Performs the secondary structure and structural motif classifications, then summarizes the predictions. (nih.gov)
  • Fragment libraries contain rich structural information, including 1D structural properties such as secondary structure and torsion angles, and 2D structural properties such as distances and orientations between pairs of heavy atoms. (biomedcentral.com)
  • The superfamily is characterised by a similar structure (a repeated +1 topology β-barrel) and by the conservation of a remarkable structural signature. (aston.ac.uk)
  • BBA - Protein Structure and Molecular Enzymology , 1482 (1-2), 9-24. (aston.ac.uk)
  • Because apoptosis and mitochondrial function play an important role in physiology and a number of diseases, intensive investigation over the past two decades has been invested to understand in detail the structure and function of the BCL-2 proteins. (elsevierpure.com)
  • Our data suggested that, in general, structure of monomeric proteins is crucially dependent on the presence of cysteine and aromatic residues. (usf.edu)
  • These proteins provide structure and support for cells. (medlineplus.gov)
  • Impact of genetic variation on three dimensional structure and function of proteins. (lu.se)
  • The result was a very plastic structure, which can shift from trigonal to distorted tetragonal depending on the details of the surrounding protein. (lu.se)
  • The results give no support to earlier suggestions that the protein strains the Cu(II) coordination sphere into a Cu(I) like structure. (lu.se)
  • The recombinant protein in Escherichia coli lysates was labelled P2 (P2 star) to differentiate it from the comigrating adhesin protein called P2. (bmj.com)
  • In response, viruses deploy proteins as counter-counter-measures to dampen the immune response, for instance, by supressing the RIG-I signalling pathway. (embl.org)
  • We complement structural studies with in vitro polymerase enzymology and in-cell studies using mini-replicon systems, and, in collaborations, recombinant viruses and live-cell imaging. (embl.org)
  • Soil Virus Offers Insight into Maintaining Microorganisms Through a collaborative effort, researchers have identified a protein in soil viruses that may promote soil health. (doe.gov)
  • The structural organization and protein composition of lens fiber junctions isolated from adult bovine and calf lenses were studied using combined electron microscopy, immunolocalization with monoclonal and polyclonal anti-MIP and anti-MP70 (two putative gap junction-forming proteins), and freeze-fracture and label-fracture methods. (rupress.org)
  • Biochemical analysis corroborated that the LisH motif in muskelin serves as a dimerization element, and, moreover, showed that the C-terminal domain of the protein substantially stabilizes this dimerization. (uni-wuerzburg.de)
  • The class of reversibly photoswitchable FPs (rs-FPs) is of particular interest for understanding the origin of structural motions that are associated with the cis-trans and trans-cis photoisomerization reactions of these biological chromophores. (nature.com)
  • Structural function is one of biological functions of proteins. (creative-biolabs.com)
  • Messenger proteins, such as some types of hormones, transmit signals to coordinate biological processes between different cells, tissues, and organs. (medlineplus.gov)
  • The experiments also added clarity to how the normal protein and its mutated forms differ: Under normal circumstances, K-Ras is more active when it is bound to the first of two partner molecules and maintains proper control of multiple cellular functions, including the return to an inactive state. (scienceblog.com)
  • Proteins are large, complex molecules that play many critical roles in the body. (medlineplus.gov)
  • These proteins bind and carry atoms and small molecules within cells and throughout the body. (medlineplus.gov)
  • Brüschweiler has his own thoughts on what might come next, such as describing how existing drugs interact with the protein. (scienceblog.com)
  • The continuity of the SC is dependent upon an integrated matrix of lipids and structural proteins, including filaggrin (FLG). (cdc.gov)
  • Further, we have summarized the speculations made about the apparent roles of intrinsic disorder in the observed multifunctionality of flaviviral proteins. (ox.ac.uk)
  • Archaeal and eukaryotic organisms with higher levels of the C-depleted proteins were shown to have higher levels of the intrinsic disorder and lower levels of structural coverage. (usf.edu)
  • The major intrinsic protein of lens plasma membranes (MIP) was localized in single membranes and in an extensive network of junctions having flat and undulating surface topologies. (rupress.org)
  • One of the most interesting features of the GFP chromophore is that it does not require a catalytic system to be formed, but is autocatalytically assembled after protein production, requiring only molecular oxygen as a cofactor for its full maturation. (ibs.fr)
  • We evaluated consequences of 12 intragenic variants by protein modelling and molecular dynamic studies. (springer.com)
  • Precision is required when designing such drugs - interfering with a protein in the wrong way could do more harm than the disease caused by a mutation. (scienceblog.com)
  • From the perspective of protein-protein interaction work, "the most practical benefit is that with those sort of nonstructural techniques you can find out what [proteins] are interacting with each other, and, now, methods are becoming more prominent where you can start to figure out the stoichiometry of the complex - how many copies of each protein exist within the complex," he said. (genomeweb.com)
  • Identification of key protein residues involved in recognition and strength of interaction. (biocev.eu)
  • Here, we will review the available structural information of pro- and anti-apoptotic members and the structures of their interaction in homodimerization and heterodimerization. (elsevierpure.com)
  • The crude JSPI contained 76.89% protein with 58.44% solubility in aquatic solvent. (banglajol.info)
  • This cylinder-shaped protein is composed of 11 (mostly) antiparallel β strands (1 and 6 are parallel) and an alpha helix through its center containing the chromophore that is formed autocatalytically from residues Ser65-Tyr66-Gly67. (ibs.fr)
  • Our structural description of Spy1 provides important mechanistic insights that may be utilized for targeting upregulated Spy1 in cancer. (escholarship.org)
  • Can Predicted Protein 3D Structures Provide Reliable Insights into whether Missense Variants Are Disease Associated? (lu.se)
  • Spy1/RINGO (Spy1) proteins bind and activate Cdk but are resistant to canonical regulatory mechanisms that establish cell-cycle checkpoints. (escholarship.org)
  • They also analyzed large collections of protein complex structural data taken from resources including the Protein Data Bank. (genomeweb.com)