Enzyme StabilityRNA, CatalyticCatalysisPhosphoenolpyruvate Sugar Phosphotransferase SystemKineticsNucleic Acid ConformationBase SequenceHot TemperatureProtein DenaturationMutagenesis, Site-DirectedTemperatureDrug StabilityModels, MolecularMutationProtein ConformationMolecular Sequence DataAmino Acid SequenceProtein StabilityHydrogen-Ion ConcentrationEscherichia coliRecombinant ProteinsRNA StabilityThermodynamics