Proteins by circular dichroism. Medical search. Frequent questions

This document specifies measurement protocols and test conditions to determine alterations to protein secondary structure induced by their interaction with nanomaterials using ultraviolet circular dichroism (UV-CD) spectroscopy. (iso.org)
Interaction of Blenoxane and Congeners Bleomycins A2 and B2 with Human Plasma Proteins Using Circular Dichroism Spectroscopy. (bvsalud.org)
His calculations on protein circular dichroism spectroscopy, a key technique in structural biology, are the most accurate to be published. (nottingham.ac.uk)
Reason, A. "Transformation of aqueous protein attenuated total reflectance infra-red absorbance spectroscopy to transmission" Quarterly Reviews of Biophysics Discovery, 2020 , 1, E8. (warwick.ac.uk)
Thomas, O. "Automated High-Throughput Capillary Circular Dichroism and Fluorescence Spectroscopy for Rapid Determination of Protein Structure" Analytical Chemistry, 2019 , 91, 13794-13802. (warwick.ac.uk)
The peptide-surfactant interactions were studied by means of circular dichroism spectroscopy and binding isotherms, as well as by 1D and 2D NMR. (lu.se)
To probe the two-stage model, the structures of peptides representing individual transmembrane helices of the disulfide bond forming protein B have been studied in trifluoroethanol solution as well as in detergent micelles using nuclear magnetic resonance (NMR) and circular dichroism spectroscopy. (rcsb.org)
Circular Dichroism (CD) is an absorption spectroscopy technique that uses the differential absorption of left- and right-handed circularly polarised light to investigate the structure of chiral molecules. (diamond.ac.uk)
In particular, ultrafast XFEL spectroscopy is used to evaluate the methionine-S-Fe bond in cytochrome c, and its entatic control by the protein in determining function (electron transfer vs. apoptosis). (stanford.edu)
Using a combination of techniques, among which atomic force microscopy, UV-circular dichroism, X-ray diffraction and two-dimensional infrared spectroscopy, the researchers performed a structural analysis to explain the differences in the aggregation of wild-type alpha-synuclein (WT-αS) and the so-called C-terminal truncated form of the protein lacking 32 amino acids at the C-terminal end (1-108-αS). (uva.nl)
The student will learn how spectroscopy and models for intermolecular interactions can be used to understand basic properties of biomolecules such as proteins, DNA and membranes. (lu.se)

To screen more than 80 compounds for their ability to block the formation of tangles, the researchers used a powerful technique - synchrotron radiation circular dichroism - for imaging structural changes in proteins. (medicalnewstoday.com)
Moreover, variant-specific interactions with extracellular matrix (ECM) components were measured by synchrotron radiation circular dichroism and enzyme-linked immunosorbent assay. (lu.se)

In drug discovery, efficient screening of protein-drug interactions (PDIs) is hampered by the limitations of current biophysical approaches. (nature.com)
Using this YaxAB nanopore, we demonstrate label-free, single-molecule detection of interactions between the anticancer Bcl-xL protein and small-molecule drugs as well as the Bak-BH3 peptide. (nature.com)
Although protein-protein interactions (PPIs) are promising targets, drugging them is one of the key challenges in drug discovery 19 . (nature.com)
Further, biophysical experiments suggested that HomA and HomB are dimeric and most likely the cysteine residues present on surface-exposed loops participate in protein-protein interactions. (nature.com)
To date, a limited number of protein interactions with bleomycin have been studied, but the plasma binding has not yet been determined. (bvsalud.org)
Another aspect of Hirst's research focuses on the study of protein-ligand interactions, using techniques including QSAR, machine learning, neural networks, docking, molecular dynamics (MD) simulations and quantum chemistry. (nottingham.ac.uk)
Foldamer-protein interactions were screened by circular dichroism (CD). (proteopedia.org)
The crystal structure of the complex between this foldamer and HCA could be resolved at 2.1 A resolution and revealed a number of unanticipated protein-foldamer, foldamer-foldamer, and protein-protein interactions. (proteopedia.org)
Previous theories proposed that B-casein had a random or 'spaghetti'-like shape and as a result, interactions with other milk proteins were also nonspecific. (usda.gov)
In 'DNA-Protein Interactions: Principles and Protocols, Third Edition', this vital subject is brought up to date with protocols exploring the most cutting-edge developments in the field, including in vivo and genome-wide interaction techniques. (nhbs.com)
Comprehensive and authoritative, 'DNA-Protein Interactions: Principles and Protocols, Third Edition' serves as an ideal guide for all those exploring this dynamic, essential, and increasingly affordable area of research. (nhbs.com)
Electrophoretic Mobility Shift Assays for the Analysis of DNA-Protein Interactions Manon Gaudreault, Marie-Eve Gingras, Maryse Lessard, Steeve Leclerc, and Sylvain L. Guerin 3. (nhbs.com)
Footprinting DNA-Protein Interactions in Native Polyacrylamide Gels by Chemical Nucleolytic Activity of 1,10-Phenanthroline-Copper Athanasios G. Papavassiliou 14. (nhbs.com)
Current work is directed towards the study of protein-membrane interactions over all timescales and the preparation of asymmetric lipid membranes for biophysical studies of membrane activity (funded by an EPSRC programme grant). (dur.ac.uk)
The key factors in clot formation are the chemical and physical nature of the surfaces and their interactions with the blood components, such as platelets and plasma proteins. (springer.com)
These observations may indicate that in the native protein, tertiary interactions additionally stabilize the secondary structure of the individual transmembrane helices. (rcsb.org)
Beamline B23 for Circular Dichroism at Diamond is a life sciences, chemistry and material science beamline for investigating and observing structural, functional and dynamic interactions in elements such as proteins, nucleic acids, nanoparticles and chiral molecules. (diamond.ac.uk)
MRD is among the few methods that can provide molecular-level informa- tion about protein solvation in native as well as denatured states, and it is used here to simultaneously monitor the interactions of urea and water with the unfolding protein. (lu.se)
1 nsec) interactions of urea and water with the native and denatured protein. (lu.se)
Solvent denaturation is a result of altered protein-solvent vorably with water-peptide interactions. (lu.se)
If this is the case, interactions, but it is not clear whether denaturants like urea then solvent denaturation can be driven simply by the ex- act directly by binding to the protein surface or indirectly by posure of more binding sites in the denatured protein perturbing solvent-mediated hydrophobic interactions or by (Schellman 1987). (lu.se)
We mostly used molecular mechanics (MM) in order to model the protein-ligand interactions, which is more approximate than quantum-mechanical (QM) methods, but necessary to reduce the computational cost when doing calculations on protein-ligand systems, which often contain tens of thousand of atoms. (lu.se)
Future work in this study might also include investigation of the surface and membrane interactions, as well as analysis of the protein dynamics. (lu.se)

Here, we explore this aspect of the protein binding capacity of bleomycin to the two most abundant plasma proteins , human serum albumin (HSA) and α1- acid glycoprotein (AGP), which are known to bind and to be carriers of many drug molecules using spectroscopic techniques , such as circular dichroism , UV-vis absorbance, and fluorescence . (bvsalud.org)
Particular expertise in circular and linear dichroism, fluorescence. (warwick.ac.uk)
Three fluorinated metformin derivatives (MTF1, MTF2 and MTF3) were synthesized and investigated for their human bloodstream - human serum albumin (HSA) interaction studies by multi-spectroscopic techniques (circular dichroism, steady state, time-resolved and synchronous fluorescence), combined with molecular docking and quantum chemical calculation. (amrita.edu)

Circular dichroism (CD) is a powerful spectroscopic technique used to study the changes in the structure and conformation of a protein. (utmb.edu)
His research spans a wide range, from the quantum chemistry of small molecules and the spectroscopic properties of proteins, to the application of state-of-the-art statistical and computer science methodology to problems in bioinformatics, drug design and sustainable chemistry. (nottingham.ac.uk)
Circular dichroism spectroscopic studies show changes in tertiary and secondary structure on deletion of the sequence. (nih.gov)
Metal sites in biology often exhibit unique spectroscopic features that reflect novel geometric and electronic structures imposed by the protein that are key to reactivity. (stanford.edu)

The chosen application is finding fractions of secondary structures of proteins from circular dichroism (CD) spectra employing singular value decomposition, SVD, to obtain the matrix B and its pseudo-reciprocal. (scirp.org)
Deriving protein secondary structure content from CD spectra has changed from postulating component spectra of the characteristic structures to the use of the secondary structure fractions from X-ray data as a basis for the mathematical prediction of the component spectra representation of the CD database. (scirp.org)
Far- and near-UV circular dichroism (CD) spectra were used to study the unique unfolding behavior of B-casein. (usda.gov)
SOMCD: method for evaluating protein secondary structure from UV circular dichroism spectra. (google.be)
Circular dichroism spectra were computed using the SESCA program. (nsf.gov)

111 Pages Report] The market for protein stability analysis is expected to grow from USD 1.2 billion in 2018 to USD 2.1 billion by 2024, at a CAGR of 10.0 % during the forecast period. (marketsandmarkets.com)
The growth of the protein stability analysis market is primarily driven by the growing adoption of open innovation models in pharmaceutical & biotechnology companies and increasing pharmaceutical outsourcing. (marketsandmarkets.com)
However, the high cost of protein stability analysis systems is expected to restrain the growth of this market to a certain extent. (marketsandmarkets.com)
On the basis of products, the protein stability analysis market is divided into reagents & assay kits, instruments, consumables & accessories, and software. (marketsandmarkets.com)
The large share of this market segment can be attributed to the high consumption of reagents and assay kits in protein stability analysis techniques, increasing protein pharmaceutical R&D, and increased government funding for life science research. (marketsandmarkets.com)
The extensive use of protein stability analysis in pharmaceutical & biotechnology companies for drug discovery applications will drive the market during the forecast period. (marketsandmarkets.com)
By end user, the protein stability analysis market is segmented into pharmaceutical and biotechnology companies, contract research organization, and academic & research institutes. (marketsandmarkets.com)
The large share of this segment is primarily due to the extensive use of protein stability analysis in pharmaceutical & biotechnology companies for drug discovery applications. (marketsandmarkets.com)
Stringent regulations for pharmaceutical drug development and manufacturing are also supporting the adoption of protein stability analysis instruments in this end-user segment. (marketsandmarkets.com)
The protein stability analysis market is segmented into four major regions, namely, North America, Europe, the Asia Pacific, and the rest of the World. (marketsandmarkets.com)
The availability of funds for research from various public and private sector organizations and increasing research activities for drug development are the major factors driving the North American market for protein stability analysis. (marketsandmarkets.com)
This research report categorizes the protein stability analysis market based on Product, Technique, End User, and Region. (marketsandmarkets.com)
Does the protein require co-factors for stability or functionality? (embl.org)
Does the protein require interaction partners for stability (→ co-expression)? (embl.org)
1980. The conformational stability of ribosomal protein L7/Ll2: The effects of pH, temperature and guani¬dinium chloride. (mote.org)
A fundamental understanding of protein stability and the mechanism of denaturant action must ultimately rest on detailed knowledge about the structure, solvation, and energetics of the denatured state. (lu.se)

These include electron paramagnetic resonance (EPR), resonance Raman, FTIR, and UV/visible/near-IR absorption and natural and magnetically induced circular dichroism (CD and MCD). (uga.edu)
The authors report on very large Faraday rotation of light polarization being due to the magnetically induced circular birefringence. (oldcitypublishing.com)

Such studies can yield a relevant information on the excitonic coupling, octant rule or the phenomenological relationship between the far-UV circular dichroism and the secondary structures of chiral molecules like proteins he has studied. (oldcitypublishing.com)

This document does not apply to the characterization of conformational changes of disordered proteins. (iso.org)
PEPCF expresses proteins in bacteria, insect and mammalian cells and uses a variety of chromatographic and biophysical techniques for protein purification and characterization. (embl.org)
His scientific work involved the characterization of biopolymers such as DNA, proteins and collagen by optical methods such as circular dichroism and light scattering. (wikipedia.org)
1991. Isolation and characterization of fatty acid binding protein in the liver of the nurse shark, Ginglymostoma cirratum. (mote.org)

Contrary to globular proteins, B-casein appears to adopt more compact conformation at elevated temperature, which provides further evidence to support our previous hypothesis of cold denaturation. (usda.gov)
However, the conformation of some amino acid side chains differs between the structures of peptide and full-length protein. (rcsb.org)
1979. The conformation of Escherichia coli ribosomal protein L7/Ll2 in solution: hydrodynamic, spectro¬scopic, and conformation prediction studies. (mote.org)
The infectious agent in the prion disease is composed mainly or entirely of an abnormal conformation of a host-encoded glycoprotein called the prion protein. (medscape.com)
The replication of prions involves the recruitment of the normally expressed prion protein, which has mainly an alpha-helical structure, into a disease-specific conformation that is rich in beta-sheet. (medscape.com)

Heat shock protein 47 (HSP47) is an endoplasmic reticulum (ER)-resident collagen-specific chaperone and essential for proper formation of the characteristic collagen triple helix. (uni-koeln.de)
Among chiral materials are such important biopolymers like deoxyribonucleic acid (DNA), collagen as well as proteins. (oldcitypublishing.com)

Analyzed protein concentration in cellular extracts with BSA assay, and protein expression by Western Blotting. (livecareer.com)

Cysteine-Selective Modification of Peptides and Proteins via Desulfurative C-C Bond Formation CHEMISTRY-A EUROPEAN JOURNAL. (nottingham.ac.uk)
The reason why Peter Lansbury and others used the term 'natively unfolded' for a-synuclein and other proteins, especially peptides, is that they do not exist, to a significant degree, in the same kind of stable structure that larger native proteins like myoglobin do. (alzforum.org)

Based on primary sequence comparisons, β subunits are predicted to be modular structures composed of five domains (A-E) that are related to the large family of membrane-associated guanylate kinase proteins. (jneurosci.org)
Maggie has strong expertise in synchrotron-based techniques such as small angle X-ray scattering (SAXS) and circular dichroism (CD) techniques, which she uses to develop new methods to study self-assembly in biologically relevant systems, and the structures of proteins and DNAs at membrane interface. (edu.au)
Peptide structures in TFE were compared to the structures of full-length protein obtained by X-ray crystallography and NMR. (rcsb.org)
Electronic Circular Dichroism Imaging (ECDi) is one of the key techniques available to determine the configuration of small molecules, particularly chiral active pharmaceutical ingredients (APIs), and is now a vital tool in chemistry, physics and materials science providing unique information on chiral structures consisting of many molecules (supramolecular). (diamond.ac.uk)
Overall, this implementation of the US-SOMO-AF database should already aid in rapidly evaluating the consistency in solution of a relevant portion of AlphaFold predicted protein structures. (nsf.gov)

H. pylori attaches and colonizes to the human epithelium using some of their outer membrane proteins (OMPs). (nature.com)
Outer membrane proteins (OMPs) of H. pylori play a crucial role in the host-pathogen interaction, virulence and pathogenesis. (nature.com)
Hom (Helicobacter outer membrane) family of OMPs in H. pylori consists of four members (HomA, B, C and D). In the H. pylori genome jhp 0870 open-reading frame (ORF) that codes for HomB outer membrane protein is associated with many stomach diseases and is a novel co-marker for peptic ulcer disease (PUD) 9 , 10 . (nature.com)
Outer membrane proteins (OMPs) are distinctive features of Gram-negative bacteria. (nature.com)
Biomacromolecule structure and function including DNA, membrane proteins and fibrous proteins including application to bacterial cell division. (warwick.ac.uk)
The behaviour of membrane proteins in membranes is being examined using a range of analytical approaches. (dur.ac.uk)
The membrane-binding properties of proteins from enveloped viruses are being characterised by a combination of microscopy, chemical biology and structral biology approaches (with Dr R. P. Yeo, Biological Sciences, Durham University). (dur.ac.uk)
This has led to the structure determination for RSV M protein (in collaboration with Dr V. Money), 3 and studies of membrane bindign using tensiometry, electron microscopy, circular dichroism and Brewster angle microscopy. (dur.ac.uk)
Brewster angle microscopy has been used to characterise the association of peripheral membrane proteins with lipid monolayers (with Dr A Beeby, Durham University). (dur.ac.uk)
The two-stage model for membrane protein folding postulates that individual helices form first and are subsequently packed against each other. (rcsb.org)
The extent of α-helical secondary structure coincided well, lending support for the two-stage model for membrane protein folding. (rcsb.org)
1978. Altered erythrocyte membrane proteins in sickle cell patients associated with the severity of the disease. (mote.org)

Completing the Immunological Fingerprint by Refractory Proteins: Autoantibody Screening via an Improved Immunoblotting Technique. (molvisindex.org)

1978. The effects of pH and temperature on the circular dichroism of human erythrocyte membranes. (mote.org)

We also collaborate with other groups for Mossbauer, electron-nuclear double resonance (ENDOR), X-ray absorption and X-ray crystallography studies of proteins prepared in our laboratory. (uga.edu)

The CusB protein is part of the CusCBA periplasmic Cu(I) efflux system in Gram-negative bacteria, and it was recently reported to play a key role in the functioning of the whole CusCBA system, in which conformational changes as well as the assembly/disassembly process control the opening of the transporter. (biorxiv.org)
Finally F. Hache presents a paper on using time-resolved circular dichroism method to study the dynamics of conformational changes in molecules. (oldcitypublishing.com)

The near-UV CD (250-320 nm) monitors changes in the vicinity of aromatic amino acids in a protein, investigating the tertiary structure of proteins. (utmb.edu)
Characteristic bands of aromatic residues (Trp, Tyr, and Phe) in the near-UV region can be used to examine the effect of mutations on the tertiary structure of proteins. (utmb.edu)
Monitored secondary and tertiary structural changes of the protein as a function of denaturant using circular dichroism (CD) and Tryptophan. (livecareer.com)

Insights from the work on formamide and other small amides have been applied to studies of proteins and have led to significant improvements in the calculation of the circular dichroism of proteins from first principles. (nottingham.ac.uk)

Low-temperature magnetic circular dichroism studies of iron-sulfur proteins. (uga.edu)
17O and 2H magnetic relaxation dispersion (MRD) to study urea-induced denaturation of intestinal fatty acid-binding protein (I-FABP). (lu.se)

In 2020, Maggie was awarded the Victoria Fellowship by the Victorian State Government, Australia, to undertake a study mission of designing super-swollen lipid nanoparticles for protein and gene delivery in four European countries and two European synchrotron facilities. (edu.au)

eLife , 2016) implied that the N-terminus of the magnesium transporter A (MgtA) protein (from hereon called Keif) is intrinsically disordered, but the advantage of this disordered feature to the function of the protein is still unknown. (lu.se)

This may be the water molecule buried near the small hydrophobic folding core at the D-E turn in the native protein. (lu.se)
hydrophobic stabilization of the folded protein. (lu.se)

The results showed that bleomycin binds to plasma proteins with an order-of- magnitude higher affinity for AGP than HSA. (bvsalud.org)
The initial biological response of blood to a foreign surface is the rapid adsorption of plasma proteins, which is followed by platelet adhesion and activation, and ultimately thrombus formation. (springer.com)

Identification of Nucleic Acid High Affinity Binding Sequences of Proteins by SELEX Philippe Bouvet 12. (nhbs.com)
Highly divergent hypotheses have been put forward regarding the makeup of the prions, including that they consist of nucleic acid only or protein only, are lacking both protein and nucleic acid, or are a polysaccharide. (medscape.com)

Visible CD (350-700 nm) is used for monitoring the interaction of prosthetic groups (various metal ions) with proteins. (utmb.edu)

The N-terminal domain of BteA is multifunctional, acting as a docking platform for its cognate chaperone (BtcA) in the bacterium, and targeting the protein to lipid raft microdomains within the eukaryotic host cell. (nih.gov)

The long funnel-shaped structure and nanofluidic characteristics of the YaxAB nanopore enable the electro-osmotic trapping of diverse folded proteins and high-resolution monitoring of PDIs. (nature.com)
Far-UV CD (190-250 nm) is used to measure the secondary structure of proteins, which monitors any change in the peptide backbone. (utmb.edu)
In order to investigate the role of this motif in the structure and function of sHsps, we have made constructs deleting this sequence from alpha A- and alpha B-crystallin, overexpressed, purified, and studied these engineered proteins. (nih.gov)
Structure of a Complex Formed by a Protein and a Helical Aromatic Oligoamide Foldamer at 2.1 A Resolution. (proteopedia.org)
This would fundamentally change our conventional thinking about protein structure-function relationship, no? (alzforum.org)
I do not think there is a contradiction to the conventional thinking of protein structure-function relation. (alzforum.org)
Recent spectacular advances by AI programs in 3D structure predictions from protein sequences have revolutionized the field in terms of accuracy and speed. (nsf.gov)
The resulting "folding frenzy" has already produced predicted protein structure databases for the entire human and other organisms' proteomes. (nsf.gov)
Thus, in this study, the structure of the Keif peptide part have been investigated by using both atomisic molecular dynamics (MD) and coarse-grained Monte Carlo (MC) simulations, as well as different experimental techniques such as circular dichroism (CD, ongoing) and small-angle X-ray scattering (SAXS). (lu.se)

The crystallization conditions included approximately 25% dioxane, and subsequent experiments by circular dichroism and sedimentation velocity analytical ultracentrifugation indicated that dioxane increases the helicity and oligomerization state of the designed protein. (rcsb.org)

Although ApoA-I amyloidosis is systemic, the different amyloidogenic variants show a preferential tissue accumulation that appears to correlate with the location of the mutation in the protein sequence and with the local extracellular microenvironment. (lu.se)

A laboratory study has found that the asthma drug salbutamol prevents the formation of tangles of fibrous protein that are a hallmark of Alzheimer's disease. (medicalnewstoday.com)

Moreover, a molecular-level understanding of iron-sulfur cluster biogenesis is crucial for understanding a variety of human diseases involving anemias, myopathies and ataxias that arise from defects in Fe-S cluster biogenesis proteins. (uga.edu)
Research on Fe-S cluster biosynthesis in the Johnson group currently involves investigating the molecular mechanisms of Fe-S cluster assembly on scaffold proteins and the subsequent intact cluster transfer to acceptor or carrier proteins. (uga.edu)
See also David Thirumalai's Emerging Ideas on the Molecular Basis of Protein and Peptide Aggregation ' (.pdf). (alzforum.org)
In the former projects atomistic and coarse-grained molecular dynamics and Monte Carlo simulations will be used together with experimental techniques such as scattering and circular dichroism, whereas the third project is focused on atomistic simulations solely. (lu.se)
1995), the molecular mechanism and the peptide group, suggesting that urea-peptide inter- whereby urea unfolds proteins has not been established. (lu.se)

Currently we do not offer protein expression in other host organisms, but we can purify proteins expressed in other host organisms (e.g. yeast, hybridoma cell lines, …) if you are able to provide us with the expression cell pellet (intracellular proteins) or with the cell culture supernatant (secreted proteins). (embl.org)
Rapid detection of intracellular SH2D1A protein in cytotoxic lymphocytes from patients with X-linked lymphoproliferative disease and their family members. (lu.se)

The therapeutic efficacy of drugs is mediated by physical interaction with their cognate targets (mainly proteins). (nature.com)
Strong extrinsic CD bands of the extrinsic chromophore and intrinsic CD signals of protein can also be used to monitor protein-ligand interaction. (utmb.edu)

A protein called calmodulin, that binds calcium ions, has an important role in the first of these pathways. (elifesciences.org)
Further tests on salbutamol suggested that it binds to individual tau molecules, preventing them from forming "nuclei" around which other protein molecules can aggregate. (medicalnewstoday.com)

While the binding did not to appear to affect protein conformations in solution, extended incubation of the amyloidogenic variants in the presence of different ECM components resulted in different aggregation propensity and aggregation patterns. (lu.se)
These amyloid plaques are immunoreactive with antibodies to the prion protein and do not immunoreact with antibodies to other amyloidogenic proteins, such as the amyloid-beta (which is deposited in Alzheimer disease). (medscape.com)

When we're taught biochemistry, we see proteins in their physiologically active forms. (alzforum.org)

In the search of molecules that could recognize sizeable areas of protein surfaces, a series of ten helical aromatic oligoamide foldamers was synthesized on solid phase. (proteopedia.org)
In healthy brain cells, tau proteins help stabilize the internal network of microscopic tubes, or "microtubules," that transports nutrients and other molecules around nerve cells. (medicalnewstoday.com)

After we receive the sequence-verified expression constructs, we will first perform small scale expression and purification tests in which we assess the expression level, the solubility and the protein enrichment after a small scale affinity purification. (embl.org)
After harvesting the V1 virus, we perform small scale expression and purification tests, in which we assess the expression level, the solubility and the protein enrichment after a small scale affinity purification. (embl.org)

Iron-sulfur protein maturation in Helicobacter pylori:Identifying a Nfu-type cluster carrier protein and its iron-sulfur protein targets. (uga.edu)

Here, we develop a biological nanopore sensor for single-molecule detection of proteins and PDIs using the pore-forming toxin YaxAB. (nature.com)
The unique biological functions of milk proteins arise directly from their three-dimensional shape, which in turn determines the properties of products made from them. (usda.gov)
Post-translational modifications to aquaporin-0 are being examined by mass spectrometry and reveal a diverse lipidation profile at each of the two lipidation sites of the protein (with Prof. R. A. Quinlan, Biological Sciences, Durham University). (dur.ac.uk)

Protein Folding and Neurodegeneration: Biophysics to the Rescue? (alzforum.org)
This does not change our view of protein folding in the least. (alzforum.org)

BteA, a 69-kDa cytotoxic protein, is a type III secretion system (T3SS) effector in the classical Bordetella, the etiological agents of pertussis and related mammalian respiratory diseases. (nih.gov)
Data indicated that ApoA-I variants exerted a cytotoxic effect in a time and cell-type-specific manner that seems to be due to protein accumulation in lysosomes. (lu.se)
Using a combination of experimental methods they show that a cytotoxic C-terminal truncated form of the alpha-synuclein protein that is abundant in vivo, aggregates into more strongly twisted fibrils that are more exposed to water. (uva.nl)

The constituent proteins are normally synthesised in specialised glands where the epithelial cells are responsible for the biosynthesis. (bioone.org)

The reports on base sequences of spider silk protein genes have gained importance as the mechanical properties of silk fibers have been revealed. (bioone.org)

Identification of Sequence-Specific DNA-Binding Proteins by Southwestern Blotting Simon Labbe, Jean-Francois Harrisson, and Carl Seguin 13. (nhbs.com)

Role of the conserved SRLFDQFFG region of alpha-crystallin, a small heat shock protein. (nih.gov)

Additionally, we compared how MD and grand-canonical Monte Carlo (GCMC) can be used to assess dynamics and thermodynamics of protein-ligand binding for both buried and solvent-exposed binding sites. (lu.se)

Evaluation of different methods to calculated reduction potentials for heme proteins. (lu.se)

In the present study, we explored the structural aspect of HomA and HomB proteins using various computational, biophysical and small-angle X-ray scattering (SAXS) techniques. (nature.com)

Do you know whether there are disulfide bonds present in the native protein? (embl.org)
Does the native protein have any post-translational modifications? (embl.org)

Her research also takes an applied and multidisciplinary approach to solve outstanding biomedical problems such as nanotechnology-enabled targeted drug delivery, biomedical imaging, protein and gene delivery, and functional food colloids. (edu.au)
Development of this important new tool was only made possible by the combination of Electronic Circular Dichroism (ECD) imaging together with the highly collimated microbeam generated at the Diamond B23 beamline which allowed higher spatial resolutions that were previously unattainable with earlier bench top instruments. (diamond.ac.uk)

Thermal and chemical denaturation data indicated the design was significantly more stable than was the wild-type protein. (rcsb.org)

Organisms4

Chemicals and Drugs81

Analytical, Diagnostic and Therapeutic Techniques and Equipment41

Phenomena and Processes58

Disciplines and Occupations5

Technology, Industry, Agriculture7

Information Science4

Health Care3

Spectroscopy11

Synchrotron radiation circular dichroism2

Interactions23

Fluorescence3

Spectroscopic4

Spectra5

Stability16

Magnetically induced circular2

Chiral molecules1

Characterization4

Conformation5

COLLAGEN2

Assay1

Peptides2

Structures5

Membrane12

Fingerprint1

Membranes1

Absorption1

Conformational2

Tertiary3

Studies of proteins1

Magnetic2

Nanoparticles1

Intrinsically disordered1

Hydrophobic2

Plasma proteins2

Nucleic2

Metal ions1

Lipid1

Structure9

Analytical1

Mutation1

Fibrous1

Molecular5

Intracellular2

Interaction2

Binds2

Amyloidogenic2

Biochemistry1

Molecules2

Solubility2

Helicobacter1

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Sequences1

Sequence1

HEAT-SHOCK-P1

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Native protein2

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Denaturation1