• Here, folding was studied for a β-barrel membrane protein, outer membrane protein A (OmpA) from Escherichia coli, in the absence and presence of two other preinserted integral proteins, BamA of the β-barrel assembly machinery complex (BAM) from E. coli and FomA from Fusobacterium nucleatum. (uni-konstanz.de)
  • protein_coding" "AAC73139","fkpB","Escherichia coli","FKBP-type peptidyl-prolyl cis-trans isomerase (rotamase) [Ensembl]. (ntu.edu.sg)
  • protein_coding" "AAC73161","apaG","Escherichia coli","protein associated with Co2+ and Mg2+ efflux [Ensembl]. (ntu.edu.sg)
  • protein_coding" "AAC73194","ftsL","Escherichia coli","membrane bound cell division leucine zipper septum protein [Ensembl]. (ntu.edu.sg)
  • protein_coding" "AAC73196","murE","Escherichia coli","UDP-N-acetylmuramoyl-L-alanyl-D-glutamate:meso-diaminopimelate ligase [Ensembl]. (ntu.edu.sg)
  • protein_coding" "AAC73197","murF","Escherichia coli","UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine ligase [Ensembl]. (ntu.edu.sg)
  • protein_coding" "AAC73288","bamA","Escherichia coli","BamABCDE complex OM biogenesis outer membrane pore-forming assembly factor [Ensembl]. (ntu.edu.sg)
  • protein_coding" "AAC73290","lpxD","Escherichia coli","UDP-3-O-(3-hydroxymyristoyl)-glucosamine N-acyltransferase [Ensembl]. (ntu.edu.sg)
  • protein_coding" "AAC73627","ppiB","Escherichia coli","peptidyl-prolyl cis-trans isomerase B (rotamase B) [Ensembl]. (ntu.edu.sg)
  • The current study elucidates and compares the changes in the membrane proteome of Escherichia coli in response to a range of alcohols. (biomedcentral.com)
  • In this regard, Escherichia coli is of special interest, since it is genetically tractable and the most well-understood bacterial species that is widely considered for commercial biofuel production [ 6 , 7 ]. (biomedcentral.com)
  • Antibacterial and detoxifying activity of NZ17074 analogues with multi-layers of selective antimicrobial actions against Escherichia coli and Salmonella enteritidis. (peertechzpublications.org)
  • Crystal structure and mechanism of the Escherichia coli ArnA (PmrI) transformylase domain. (colorado.edu)
  • Crystal structure of Escherichia coli ArnA (PmrI) decarboxylase domain. (colorado.edu)
  • Folding of OmpA from its complex with the periplasmic chaperone Skp into bilayers composed of phosphatidylethanolamine and phosphatidylcholine was inhibited in the absence of BamA but facilitated when BamA was present, indicating an interaction of Skp-OmpA complexes with BamA. (uni-konstanz.de)
  • 2015 . The crystal structure of full-length E. coli YidC revealed that a hydrophilic groove, formed by five transmembrane helices, is a conserved structural feature of YidC, as compared to the previous YidC structure from Bacillus halodurans , which lacks a periplasmic domain. (tcdb.org)
  • 2022 ). The YidC transmembrane (TM) groove is essential for a high- affinity interaction, and the hydrophilic nature of the YidC groove plays an important role in protein transport across the cytoplasmic membrane bilayer to the periplasmic side. (tcdb.org)
  • Gopinath A, Joseph B. Conformational flexibility of the protein insertase BamA in the native asymmetric bilayer elucidated with ESR spectroscopy. (fu-berlin.de)
  • The subunit BamA is located in the outer membrane and exposed to the outer side of the bacteria, which provides a great target for new drugs. (medicinetechnews.com)
  • Folding of β-barrel membrane proteins, either from a urea-unfolded form or from chaperone-bound aqueous forms, has been characterized for pure lipid bilayers. (uni-konstanz.de)
  • Three different preformed lipid membranes of phosphatidylcholine were prepared to compare the folding kinetics of OmpA, namely, proteoliposomes containing either BamA or FomA and pure liposomes. (uni-konstanz.de)
  • Urea-unfolded OmpA folded faster into phosphatidylcholine bilayers containing FomA than into pure lipid bilayers, but the kinetics of OmpA folding and insertion were fastest for bilayers containing BamA. (uni-konstanz.de)
  • Incorporation of BamA into lipid bilayers composed of phosphatidylcholine and phosphatidylethanolamine greatly weakened the inhibiting effect of phosphatidylethanolamine on the folding of OmpA. (uni-konstanz.de)
  • Gram-negative bacteria is particularly resistant to antibiotics because of its cells' impermeable lipid-based outer membrane. (medicinetechnews.com)
  • 2018 ). Through YidC, proteins are inserted into the lipid bilayer via the SecYEG-dependent complex, but YidC functions as a chaperone in protein folding processes. (tcdb.org)
  • Mitochondria import nuclearly-encoded proteins, made in the cell cytoplasm, into the mitochondrial matrix where their mitochondrial targeting sequences are removed by proteolysis. (tcdb.org)
  • 2001 ). Mitochondria have two, one for co-translational, and one for post-translational insertion of membrane proteins. (tcdb.org)
  • Mitochondria inherited three inner membrane translocases Sec, TAT and Oxa1 (YidC) from its bacterial ancestor. (tcdb.org)
  • Beta-barrel proteins form the gates of the cell wall for importing nutrition and secreting important biological molecules. (medicinetechnews.com)
  • The beta-barrel assembly machinery (BAM) is responsible for building the gates (beta-barrel proteins) in the cell wall. (medicinetechnews.com)
  • Scientists studied the gram-negative bacteria E.coli, in which the beta-barrel assembly machinery contains five subunits - known as BamA, BamB, BamC, BamD and BamE. (medicinetechnews.com)
  • They then export some of these proteins as well some mitochondrially-encoded proteins to the inter membrane space, or they insert them into the inner membrane. (tcdb.org)
  • Kugele A, Ketter S, Silkenath B, Wittmann V, Joseph B,* Drescher M*. In situ EPR Spectroscopy of a Bacterial Membrane Transporter using an Expanded Genetic Code. (fu-berlin.de)
  • Selective High-Resolution Detection of Membrane Protein-Ligand Interaction in Native Membranes using Trityl - Nitroxide PELDOR. (fu-berlin.de)
  • Ketter S, Dajka M, Rogozhnikova O, Dobrynin S, Tormyshev VM, Bhagryanskaya EG, Joseph B. In situ distance measurements in a membrane transporter using maleimide functionalized orthogonal spin labels and 5-pulse electron-electron double resonance spectroscopy. (fu-berlin.de)
  • Collection of 240 intestinal samples from 15 healthy individuals using the device and subsequent multi-omics analyses identified significant differences between bacteria, phages, host proteins and metabolites in the intestines versus stool. (bvsalud.org)
  • The impact of preinserted integral proteins from biomembranes has not been examined in biophysical comparisons, but this knowledge is important for the characterization of protein assembly machinery in membranes to distinguish specific effects from unspecific effects. (uni-konstanz.de)
  • In the present study, we explored the structural aspect of HomA and HomB proteins using various computational, biophysical and small-angle X-ray scattering (SAXS) techniques. (nature.com)
  • Further, biophysical experiments suggested that HomA and HomB are dimeric and most likely the cysteine residues present on surface-exposed loops participate in protein-protein interactions. (nature.com)
  • At different stages of the insertion process, conformational changes in YidC's TM domain and membrane core have a mechanistic effect on Pf3 coat protein insertion. (tcdb.org)
  • Ketter S, Gopinath A, Joseph B. Conformational heterogeneity of β-barrel membrane proteins observed in situ using orthogonal spin labels and pulsed ESR spectroscopy. (fu-berlin.de)
  • We found that the five subunits form a ring structure and work together to perform outer membrane protein insertion using a novel rotation and insertion mechanism. (medicinetechnews.com)
  • Bacteria also export (to the periplasm) N-tails of membrane proteins synthesized without leader sequences by a Sec (Type IIPS)-independent mechanism. (tcdb.org)
  • At least for those proteins that contain their N-termini in the intermembrane space, export is mediated by the Oxa1p export machinery. (tcdb.org)
  • Insertion of a membrane protein via the Oxa1p export machinery follows the 'positive-inside' rule for membrane protein topology. (tcdb.org)
  • Cryogenic transmission electron microscopy and tomography showed parallel membrane-bound segments that are likely cells, encapsulated by an S-layer-like periodic surface covering. (bvsalud.org)
  • Alanine scanning of MS2 coat protein reveals protein-phosphate contacts involved in thermodynamic hot spots. (colorado.edu)
  • 2007 ). Several Oxa1-type insertases in the ER serve as catalytically active core subunits in the ER membrane protein complex (EMC), the guided entry of tail-anchored (GET) and the GET- and EMC-like (GEL) complex. (tcdb.org)
  • investigated pathogenesis and immunological response caused by HomB protein in clinical patients and 190 H. pylori strain isolated from patients with peptic ulcer disease (PUD) or gastritis were evaluated for the clinical importance of homB . (nature.com)
  • Our study provides essential structural information of unexplored proteins of the Hom family that can help in a better understanding of H. pylori pathogenesis. (nature.com)
  • A beta-hairpin comprising the nuclear localization sequence sustains the self-associated states of nucleosome assembly protein 1. (colorado.edu)
  • The matrix exposed C-terminal α-helical domain of Oxa1 can bind mitochondrial ribosomes to facilitate co-translational insertion of proteins into the mitochondrial membrane ( Jia et al . (tcdb.org)
  • Although alcohol toxicity increased exponentially with alcohol chain length (2-6 carbon), similar stress responses were observed in the inner and outer membrane proteome of E. coli in the presence of 2-, 4- and 6-carbon alcohols at the MIC 50 . (biomedcentral.com)
  • Since alcohol primarily targets the integrity of the membrane, maintenance of outer membrane OmpC levels in the presence of alcohol might help in the survival of E. coli to higher alcohol concentrations. (biomedcentral.com)
  • HomB protein contributes to the colonization and persistence of H. pylori , and the presence of homB genes affects the number of bacteria adhering to the host cells. (nature.com)
  • Major outer membrane β-barrel protein OmpC, which is known to contribute to ethanol tolerance and membrane integrity, was notably reduced by alcohol stress. (biomedcentral.com)
  • The study provides important information about the membrane protein responses of E. coli to a range of alcohols, which can be used to develop targeted strategies for increased microbial alcohol tolerance and hence bioalcohol production. (biomedcentral.com)
  • This outer membrane acts as a defensive barrier against attacks from the human immune system and antibiotic drugs. (medicinetechnews.com)
  • OstA-like protein, LPS transport system D [Interproscan]. (ntu.edu.sg)
  • Bacteria cultured in hyperosmotic conditions and bacteria experiencing nutrient stress exhibit similar physiological changes, including metabolic shutdown, increased protein instability, dehydration, and condensation of chromosomal DNA. (bvsalud.org)
  • Legionella pneumophila macrophage infectivity potentiator protein appendage domains modulate protein dynamics and inhibitor binding. (fu-berlin.de)
  • Homologues of the yeast Oxa1 protein are found in chloroplasts of plants and in a wide variety of bacteria. (tcdb.org)