Proteins are highly conserved rna rna small interfering. Medical search. Frequent questions

SncRNAs are less than 200 nt in length, consisting of microRNAs (miRNAs), Piwi-interacting RNAs (piRNAs), endogenous small interfering RNAs (endo-siRNAs or esiRNAs) and transcription initiation RNAs (tiRNAs) [ 6 ]. (ijbs.com)
The goal of this research was to provide a highly robust and concise methodology for in-vitro screening of efficient siRNAs from a bulk to be used as a tool to protect potato plants against PVY invasion. (virosin.org)
siRNAs are produced from double-stranded RNAs of viruses and transposable elements, which are processed by the dicer nuclease, one of the essential components of the RNA-Induced Silencing Complexes (RISCs) [ 7 - 9 ]. (biomedcentral.com)
Several classes of small RNAs, including short interfering RNAs (siRNAs), microRNAs (miRNAs), and Piwi-interacting RNAs (piRNAs) have been identified. (ecmbio.com)
Measles computer virus which like NDV belongs to the family small interfering RNAs (siRNAs) consisting of three target-specific 21-nucleotide siRNAs designed to specifically knock down chicken gene expression along with control scrambled siRNA were designed and synthesized by GenePharma. (mdm-inhibitors.com)
We developed cell lines in which RanBP2 levels can be controlled through the induction of short interfering RNAs (siRNAs). (nih.gov)

These small RNAs, which include microRNA (miRNA) and several classes of small interfering RNAs (siRNA), play important roles in regulating gene expression, heterochromatin formation, and defense against invading viruses. (ttu.edu)
Qi X, Bao FS, and Xie Z. (2009) Small RNA Deep Sequencing Reveals Role for Arabidopsis thaliana RNA-Dependent RNA Polymerases in Viral siRNA Biogenesis. (ttu.edu)
Results and Discussion To study the impact and extent to which miRNA contributes to cell cycle progression in transformed phenotypes we here employed the p53-mutated human keratinocyte cell line (HACAT) and RNA interference (siRNA) techniques. (careersfromscience.org)
Small interfering RNA (siRNA)-mediated knockdown of p32 in term placental explants significantly reduced the number of Ki67-positive CTB, but did not alter CTB apoptosis or necrosis. (manchester.ac.uk)
Employing various pharmacological S1PR (S1P receptor) agonists and antagonists, along with siRNA (small interfering RNA) techniques and genetic knockout approaches, we identify the S1PR2 as the specific and necessary receptor to induce phosphorylation of ERM proteins and subsequent filopodia formation. (omicsdi.org)
and interaction of RMRP and hTERT leads to an RNA-dependent RNA polymerase activity leading to siRNA altering gene expression. (medscape.com)

The present study intended to establish MM cell lines with stably silenced SPRY2 using RNA interference technology. (spandidos-publications.com)
We have combined the proteomic analysis of Xenopus laevis in vitro-assembled chromosomes with RNA interference and live cell imaging in HeLa cells to identify novel factors required for proper chromosome segregation. (rupress.org)
For 20 years we have known about RNA interference limiting expression of genes. (phoenixrising.me)
RNA interference (RNAi) is a powerful tool for functional gene analysis which has been successfully used to downregulate the expression levels of target genes. (virosin.org)
2001. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. (virosin.org)
RNA interference by short hairpin RNAs expressed in vertebrate cells. (virosin.org)
Comparative-genomic analysis of CRISPR and cas genes leads to the hypothesis that the CRISPR-Cas system (CASS) is a mechanism of defense against invading phages and plasmids that functions analogously to the eukaryotic RNA interference (RNAi) systems. (biomedcentral.com)
More recently, small interfering RNAs are effective in inducing endogenous RNase of the RNA-induced silencing complex in the RNA interference pathway to inhibit gene expression and growth of several human viruses (5,10,11). (azd1152.com)

The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. (wikipedia.org)
Most eukaryotic organisms possess highly conserved RNA silencing machinery that generates 21- ~ 24-nucleotide small RNAs from double stranded RNA precursors. (ttu.edu)
Small RNAs have proven to be essential regulatory molecules encoded within eukaryotic genomes. (biomedcentral.com)
Circular RNAs (circRNAs), a novel type of non-coding RNAs (ncRNAs), are ubiquitously expressed in eukaryotic cells during post-transcriptional processes. (ijbs.com)
p32 is a conserved eukaryotic protein which is primarily expressed in the mitochondria and regulates cell proliferation, migration and metabolism in various tissues. (manchester.ac.uk)
Specific functional analogies are drawn between several components of CASS and proteins involved in eukaryotic RNAi, including the double-stranded RNA-specific helicase-nuclease (dicer), the endonuclease cleaving target mRNAs (slicer), and the RNA-dependent RNA polymerase. (biomedcentral.com)
There are two major, distinct forms of regulatory small RNAs involved in eukaryotic gene silencing: small interfering (si) RNAs and micro (mi) RNAs. (biomedcentral.com)
The mammalian target of rapamycin (mTOR) and phosphatidylinositol 3-kinase (PI3K) signaling pathways have been shown to control autophagy in mammalian cells (13 14 The autophagy-related proteins such as Beclin 1 are critical for the signaling pathways involved in autophagosome formation (15 16 Autophagy from monocellular eukaryotic organisms to primates is usually a housekeeping mechanism. (mdm-inhibitors.com)

The core components of RNA silencing machinery involve several evolutionarily conserved protein families, including DICER (DCR) or DICER-LIKE (DCL), ARGONAUTE (AGO), and, in some cases, RNA-DEPENDENT RNA POLYMERASE (RDR). (ttu.edu)
Real‑time quantitative polymerase chain reaction (PCR) and western blot analyses were used to detect SPRY2 expression and miR‑21 protein expression levels. (spandidos-publications.com)
The nomenclature of different promoter-associated RNA polymerase II (Pol II) species is explicitly defined in an effort to provide consistency in future literature. (nature.com)
Recent years have witnessed a sea change in our understanding of transcription regulation: whereas traditional models focused solely on the events that brought RNA polymerase II (Pol II) to a gene promoter to initiate RNA synthesis, emerging evidence points to the pausing of Pol II during early elongation as a widespread regulatory mechanism in higher eukaryotes. (nature.com)
[ 16 ] The 3' end of RMRP is essential for RNA dependent RNA polymerase acitivity of the RMRP-hTERT complex. (medscape.com)
Human adenovirus was detected by real-time and conventional polymerase chain reaction and molecular typed by partial hexon capsid protein gene sequencing. (cdc.gov)

The mammalian target of rapamycin (mTOR) is an evolutionarily highly conserved intracellular serine/threonine kinase family member. (hindawi.com)
MSY3 (MSY4, YB-2, CSDA, dbpA, ZONAB) is a member of the cold shock domain (CSD) family of proteins also known as Y-box proteins, which are evolutionarily conserved proteins that function as transcription factors and regulators of RNA metabolism and protein synthesis. (biomedcentral.com)

miRNAs are a recently discovered class of small evolutionary highly conserved non-coding RNAs of 18-25 nucleotides length which are generated by sequential processing of primary PD173955 and Mouse monoclonal to alpha Actin precursor miRNA by the ribonucleases Drosha and Dicer1. (careersfromscience.org)
The PD173955 mechanisms however by which miRNAs interfere with cell survival and programmed cell death in transformed cells are still obscure. (careersfromscience.org)
Mature microRNAs (miRNAs) are a class of small, well-conserved, non-coding RNA molecules that silence gene expression usually by interfering with mRNA stability or protein translation ( 6 , 7 ). (spandidos-publications.com)
Up to 30% of human protein-coding genes may be regulated by miRNAs ( 8 , 9 ). (spandidos-publications.com)
miRNAs are involved in biological and pathological processes including cell differentiation, proliferation, apoptosis and metabolism, and they are emerging as highly tissue-specific biomarkers with potential clinical application for defining cancer types and origins. (spandidos-publications.com)
Mapping all miRNAs to the tammar genome and comparing target genes among tammar, mouse and human, we identified 163 conserved target genes. (biomedcentral.com)
The first class of small RNAs identified were the micro RNAs (miRNAs), which are small (~22 nt) non-coding RNAs that regulate gene expression by base pairing to mRNAs where they direct either mRNA cleavage or repress translation [ 8 ]. (biomedcentral.com)

Whithout mutations we would know very little about inheritance and the existence of genes. (intechopen.com)
The involvement of viral DNA-binding proteins in the regulation of virulence genes, transcription, DNA replication, and repair make them significant targets. (mdpi.com)
Using a combination of miRNA hairpin predictions and co-mapping with miRBase entries, we identified a highly conserved cluster of miRNA genes on the X chromosome in the tammar and a total of 94 other predicted miRNA producing genes. (biomedcentral.com)
The characterization of new miRNA target genes and a unique profile for crasiRNAs has allowed for insight into multiple RNA mediated processes in the tammar, including gene regulation, species incompatibilities, centromere and chromosome function. (biomedcentral.com)
Studies of human genome demonstrate that protein-coding genes only occupy less than 2% of the entire genome [ 1 ]. (ijbs.com)
One surprise at the time was that the protein coding genes identified were far less numerous than expected. (phoenixrising.me)
One reason you may see different figures for this is that even coding regions containing genes are more likely than not to contain 'introns' within genes which are removed before the RNA transcript is spliced together and passed to protein synthesis. (phoenixrising.me)
The three genes encoding these proteins were inserted individually into vaccinia virus GLV-1h68 genome under the control of an identical promoter. (uni-wuerzburg.de)
All archaeal and many bacterial genomes contain Clustered Regularly Interspaced Short Palindrome Repeats (CRISPR) and variable arrays of the CRISPR-associated ( cas ) genes that have been previously implicated in a novel form of DNA repair on the basis of comparative analysis of their protein product sequences. (biomedcentral.com)
80% test accuracy) while using two novel predicted protein coding genes (Gm5067, Gm6089) as features. (fortuneonline.org)
Genetic studies have clarified that most microcephaly genes encode ubiquitous proteins involved in mitosis and in maintenance of genomic stability, but the effects of their inactivation are particularly strong in neural progenitors. (cancerindex.org)
Although pausing has been connected to extremely rapid and synchronous activation of genes, pausing is also highly associated with constitutively expressed genes that encode signalling and transcription factors. (nature.com)
Distinct signals that act through diverse targeted transcription factors can regulate different steps in the transcription pathway and provide a highly modulated transcriptional response at individual genes. (nature.com)
Jumonji (JmjC) domain proteins (see Jarid2 ) influence gene expression and chromatin organization by way of histone demethylation, which provides a means to regulate the activity of genes across the genome. (sdbonline.org)

PUCH produces small molecules called piRNAs, which act as sentinels to detect and halt TEs before they can insert themselves into new locations in our DNA. (thaimedicalnews.com)
A survey of the tammar gonadal piRNAs shows that these small RNAs are enriched in retroelements and carry members from both marsupial and tammar-specific repeat classes. (biomedcentral.com)
Another class of important small RNAs is the piRNAs. (biomedcentral.com)

These short RNAs participate in a diverse array of cellular processes including gene regulation, chromatin dynamics and genome defense. (biomedcentral.com)
As part of the genome sequencing initiative for the tammar, we have explored the evolution of each of the major classes of mammalian small RNAs in an Australian marsupial for the first time, including the first genome-scale analysis of the newest class of small RNAs, centromere repeat associated short interacting RNAs (crasiRNAs). (biomedcentral.com)
We examined each of these small RNA classes with respect to the newly assembled tammar wallaby genome for gene and repeat features, salient features that define their canonical sequences, and the constitution of both highly conserved and species-specific members. (biomedcentral.com)
This study encompasses the first analyses of the major classes of small RNAs for the newly completed tammar genome, validates preliminary annotations using deep sequencing and computational approaches, and provides a foundation for future work on tammar-specific as well as conserved, but previously unknown small RNA progenitors and targets identified herein. (biomedcentral.com)
Small RNAs play important roles in many aspects of pre- and post-transcriptional gene regulation, epigenetic modifications, chromosome segregation and genome structure. (biomedcentral.com)
The Human Genome Project found essentially all of the 'coding DNA' being transcribed into proteins back in 2003. (phoenixrising.me)
What about all that non-coding RNA (ncRNA) stuff being transcribed from DNA in the genome which does not go on to produce proteins, is it meaningless and nonfunctional? (phoenixrising.me)

Several human and animal virus diseases, generally caused by RNA viruses, have emerged in the last 40 years ( 1 , 2 ). (cdc.gov)
Dengue computer virus Dengue computer virus (DENV) is usually a single-stranded ribonucleic acid (RNA) virus belonging to the family Flaviviridae, which includes the Yellow Fever virus, West Nile computer virus, and around 70 other viruses [1,4]. (enmd-2076.com)
Schlafen proteins appear to play a role in innate immunity, the body's first line of defense against viruses and bacteria. (thaimedicalnews.com)
Some Schlafen proteins interfere with viral replication in humans, while certain viruses may use Schlafen proteins to subvert the cell's defense system. (thaimedicalnews.com)
CircRNAs were first identified in RNA viruses in the 1970s [ 12 - 14 ]. (ijbs.com)
We have determined the ability of 83 proteins of several families of RNA viruses (Paramyxoviridae, Flaviviridae, Orthomyxoviridae, Retroviridae and Togaviridae), to interact with 44 human autophagy-associated proteins using yeast two-hybrid and bioinformatic analysis. (sfr-biosciences.fr)
We found that the autophagy network is highly targeted by RNA viruses. (sfr-biosciences.fr)
Our work reveals an unexpected role of IRGM in virus-induced autophagy and suggests that several different families of RNA viruses may use common strategies to manipulate autophagy to improve viral infectivity. (sfr-biosciences.fr)

Launch Newcastle disease trojan (NDV) is normally a single-stranded nonsegmented negative-sense RNA trojan that is one of the family members (1). (mdm-inhibitors.com)

In-depth study of small non-coding RNA molecules confirmed that microRNA (miR) has an important regulatory role in cell proliferation, differentiation, metabolism, apoptosis and development processes ( 3 ). (spandidos-publications.com)
p53 arrests cells during G1 progression and G1/S transit by inducing the CDK-inhibitory protein p21CIP1 and microRNA cluster miR-34a. (careersfromscience.org)
Argonaute proteins participate in various steps of microRNA-mediated gene silencing, such as repression of translation and mRNA turnover. (ecmbio.com)

Xie Z ., Cheng H. (2017) Interplay and transition between small RNA-directed posttranscriptional and transcriptional gene silencing in plants. (ttu.edu)
A species of small antisense RNA in post transcriptional gene silencing in plants. (virosin.org)
In this study, one of these EGS variants was used to target the overlapping region of the mRNAs encoding HCMV essential immediately-early (IE) proteins IE1 and IE2, which are the viral major transcriptional activators responsible for activation of viral gene expression (1). (azd1152.com)
MSY3 is a DNA binding protein, which acts as a transcriptional repressor. (biomedcentral.com)
Polycomb Group (PcG) proteins maintain transcriptional repression throughout development, mostly by regulating chromatin structure. (sdbonline.org)

The function is performed by identifying 3′-UTRs (untranslated regions) of target mRNAs with conserved complementarities to the seed (nucleotides 2-7) of the miRNA. (spandidos-publications.com)
[ 15 ] The second consists of single nucleotide substitutions and other changes that involve at most 2 nucleotides in highly conserved regions of the gene.These are considered leaky mutations and result in variable expression of the gene, which may explain the variable phenotype seen in cartilage-hair hypoplasia. (medscape.com)

When very similar molecules are produced by DNA sequences found in both humans and in bacteria that live in hot springs you should guess that those sequences are highly conserved, and for some good reason. (phoenixrising.me)

According to their differential sequences, SPRY proteins were divided into four subtypes (SPRY1, -2, -3 and -4). (spandidos-publications.com)
Part of this can be described as simple interactions between RNA sequences which pair up with single strands of RNA loose in cells, essentially jamming the machinery. (phoenixrising.me)
There is a family of proteins called argonaute ( for reasons that would take us way off-topic ) which can use short RNA sequences to identify the location on which to operate on longer strands. (phoenixrising.me)
However, these signature sequences have not so far been extensively investigated amongst orthologous Bcl-2 family proteins. (sfr-biosciences.fr)
Fig. 1: Photochemistry of light-oxygen-voltage receptors and sequences of proteins under study. (nature.com)

Bcl2l10 is an antiapoptotic member of the Bcl-2 family that has evolved rapidly throughout the vertebrate lineage and which shows conserved abundant expression in eggs and oocytes. (sfr-biosciences.fr)

Plants, therefore, provide a unique system to study the genetic diversification and functional adaptation of small RNA pathways. (ttu.edu)
2004) Genetic and functional diversification of small RNA pathways in plants. (ttu.edu)
In this study, we are interested in investigating the expression of a variety of functional classes of proteins in ESCC and its precursor lesions and characterizing the correlation of these proteins with ESCC malignant progression. (biomedcentral.com)
Another 30,000 pieces of functional information (over and above proteins) are part of cell architecture. (uncommondescent.com)
While deletion of the N-terminal extension had no significant functional impact in HeLa cells, our results suggest that the human Bcl2l10 protein evolved a calcium-binding motif in its alpha 5-alpha 6 interhelical region by acquiring critical negatively charged residues. (sfr-biosciences.fr)
A reduction of 75% in the expression of TK mRNA and protein was observed in HSV-1-infected cells that expressed these functional EGS RNAs. (azd1152.com)
We speculate that by coupling RNA processing to the status and activity of Pol II itself, the cell ensures that nascent RNA is properly protected from degradation and efficiently matures into a functional mRNA. (nature.com)
Our current studies on this complex focus on functional dissection of the multiple domains within this large protein and on interacting proteins that may be essential for the function of the RanBP2 complex. (nih.gov)

A system for stable expression of short interfering RNAs in mammalian cells. (virosin.org)

The SPRY2 protein contains 315 human amino acid residues (35 kDa), with the C-terminal residues 178-282 being rich in cysteine. (spandidos-publications.com)
Both DNA variants affect highly conserved residues of S1PR2 and are predicted to be damaging by multiple bioinformatics tools. (omicsdi.org)
As recently proposed 25 , glutamine reorientation, and signal propagation may be aided by transient rearrangements of two conserved asparagine residues that coordinate the pteridin portion of the flavin. (nature.com)

Bod1 depletion does not affect the activity or localization of Aurora B but does cause mislocalization of the microtubule depolymerase mitotic centromere- associated kinesin and prevents its efficient phosphorylation by Aurora B. Therefore, Bod1 is a novel kinetochore protein that is required for the detection or resolution of syntelic attachments in mitotic spindles. (rupress.org)
Previously we demonstrated that the sphingolipids ceramide and S1P (sphingosine 1-phosphate) regulate phosphorylation of the ERM (ezrin/radixin/moesin) family of cytoskeletal proteins [Canals, Jenkins, Roddy, Hernande-Corbacho, Obeid and Hannun (2010) J. Biol. (omicsdi.org)
In the present article, we show that exogenously applied or endogenously generated S1P (in a sphingosine kinase-dependent manner) results in significant increases in phosphorylation of ERM proteins as well as filopodia formation. (omicsdi.org)
Taken together, the results demonstrate a novel mechanism by which S1P regulates cellular architecture that requires S1PR2 and subsequent phosphorylation of ERM proteins. (omicsdi.org)
These results support the hypothesis that MSY3 phosphorylation by Akt interferes with MSY3 repression of myogenin circuit activity during muscle development. (biomedcentral.com)
Pol II pausing and release occur at a point when 5′ end RNA processing and phosphorylation of the Pol II carboxy-terminal domain occurs. (nature.com)

This finding has implications for our understanding of innate immunity and RNA-based processes that control transposable elements. (thaimedicalnews.com)
Considering the reliance of female eggs on calcium-dependent proteins and calcium-regulated processes and the exceptional longevity of oocytes in the primate lineage, we propose that this microstructural variation may be an adaptive feature associated with high maternal expression of this Bcl-2 family member. (sfr-biosciences.fr)
The sphingosine-1-phosphate receptors (S1PRs) are a well-studied class of transmembrane G protein-coupled sphingolipid receptors that mediate multiple cellular processes. (omicsdi.org)
An equally conserved, proximal glutamine processes the resultant flavin protonation into downstream hydrogen-bond rearrangements. (nature.com)

The mature miRNA is then loaded onto an Argonaute protein (Ago2 in humans) where it then interacts with and regulates the mRNA target. (biomedcentral.com)
Another argonaute protein which has been studied extensively is found in thermus thermophilus , a species of bacteria which made an important contribution to the development of PCR. (phoenixrising.me)
Among the most important components in these complexes are argonaute proteins. (ecmbio.com)

Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. (wikipedia.org)
Core, L. J., Waterfall, J. J. & Lis, J. T. Nascent RNA sequencing reveals widespread pausing and divergent initiation at human promoters. (nature.com)

Microtubule-associated protein 1 light chain 3 (LC3) the mammalian homologue of yeast Atg8 (8) is the most widely monitored autophagy-related protein (9 SL 0101-1 10 Accumulation of autophagosomes may be the outcome of either enhanced autophagosome biogenesis or disrupted trafficking to lysosomes. (mdm-inhibitors.com)

The method of tissue microarray (TMA), which could confirm the results of cDNA microarray on protein level, has been popularly used for gene expression profiling in many types of cancer including ESCC, but rarely in the consecutive stages of carcinogenesis, especially in the precursor lesions. (biomedcentral.com)

Our results demonstrate that ARMC5 was a substrate adaptor protein between full-length SREBF and CUL3-based E3 ligase, and they suggest the involvement of the SREBF pathway in the development of BMAH. (bvsalud.org)
SPRY2 is a member of the signaling pathway-specific inhibition protein sprouty (SPRY) family. (spandidos-publications.com)
Autophagy is a conserved degradative pathway used as a host defense mechanism against intracellular pathogens. (sfr-biosciences.fr)
Moreover we found that the expression of IRGM-interacting MeV-C, HCV-NS3 or HIV-NEF proteins per se is sufficient to induce autophagy, through an IRGM dependent pathway. (sfr-biosciences.fr)

Gene silencing studies with small interfering RNA demonstrated that this protein plays an essential role in coordinating chromosome replication and segregation with cytokinesis. (wikipedia.org)
The ultimate goals of our studies are to understand how these proteins enable accurate chromosome segregation and to discover how they are coordinated with each other and with other aspects of cell physiology. (nih.gov)

The first of these is Bod1, a protein conserved throughout metazoans that associates with a large macromolecular complex and localizes with kinetochores and spindle poles during mitosis. (rupress.org)

Little is currently known about how these EGS RNA variants increase their activity in directing RNase P to cleave a target mRNA. (azd1152.com)
gene expression and growth and furthermore, demonstrate the feasibility of developing effective EGS RNA variants for anti-HCMV applications by using selection procedures. (azd1152.com)
Molecular modeling predicts that these variants affect binding of sphingosine-1-phosphate (p.Arg108Pro) and G protein docking (p.Tyr140Cys). (omicsdi.org)

It mainly forms the catalytic subunits of two protein complexes, including mTOR complex 1 (mTOR complex1, mTORC1) and mTOR complex 2 (mTOR complex2, mTORC2). (hindawi.com)
These small RNAs function together with complexes called micro-ribonucleoproteins (miRNPs) to regulate gene expression by modulating mRNA translation or stability. (ecmbio.com)
Trafficking between the nucleus and cytoplasm occurs through nuclear pore complexes (NPCs), which consist of ca. thirty distinct proteins called nucleoporins. (nih.gov)

PUCH-mediated processing requires a specific molecular structure and is highly specific, ensuring that only piRNA precursors are targeted. (thaimedicalnews.com)

MZB has a very specific mechanism of action on the lymphocytes that inhibits their proliferation without interfering with purine synthesis in other cell types. (hindawi.com)

Xie Z . (2011) Biogenesis and function of virus-derived small interfering RNAs in plants. (ttu.edu)
The antiviral dsRNA-binding proteins, NF90/NF110, act as key regulators in circRNA biogenesis. (ijbs.com)
Autophagy can favor viral replication in a number of ways including assisting computer virus biogenesis egress and the translation of incoming viral SL 0101-1 RNA. (mdm-inhibitors.com)

Materials and Methods: PrP c expression was suppressed in HT29 human colon cancer cells by utilizing small-interfering RNA (si-PRNP), and cells were subsequently used to study the antiproliferative and anticancer effects of fucoidan treatment of HT29 human colon cancer cells. (iiarjournals.org)
Employing a cell cycle-wide phospho-proteomic approach we detected neglectable changes in abundance and schedule of overall and cell cycle periodic protein expression despite cell cycle arrest of Dicer1-depleted cells. (careersfromscience.org)
The effect of miR-126 expression on target proteins was assessed using miR-126 mimics or miR-126 inhibitors in ESCC cell lines. (spandidos-publications.com)
miR-126 mimics downregulated the expression of IRS-1 and GOLPH3 protein and suppressed the proliferation, migration and invasion of ESCC cells, whereas miR-126 inhibitors led to the opposite results. (spandidos-publications.com)
Studies have shown that the expression of miR-126 is downregulated in cancers such as non-small cell lung cancer and colon cancer ( 14 , 15 ), suggesting that the downregulation of miR-126 is significantly related to the occurrence and development of cancer. (spandidos-publications.com)
Analyzing the protein expression patterns of Fas, FADD, caspase 8, CDC25B, fascin, CK14, CK4, annexin I, laminin-5γ2 and SPARC would be valuable to develop rational strategies for early detection of lesions at risk in advance as well as for prevention and treatment of ESCC. (biomedcentral.com)
These conditions include but are not limited to artery or vein occlusion in diabetic retinopathy, hypoxic retina in retinopathy of prematurity, aging in age-related macular degeneration, expression of mutant proteins in inherited retinal degeneration, traumatic injury leading to retinal detachment, and light sensitivity in the case of light-induced retinal degeneration. (molvis.org)
Experimental research found a markedly decreased expression of autophagy-related proteins such as Beclin-1, Atg12-Atg5, and LC3-II in a diabetic mouse model and in a high glucose-induced podocyte injury model [ 4 ]. (hindawi.com)
Strikingly, reduction of IRGM expression using small interfering RNA impairs both Measles virus (MeV), Hepatitis C virus (HCV) and human immunodeficiency virus-1 (HIV-1)-induced autophagy and viral particle production. (sfr-biosciences.fr)
p32 mRNA and protein expression was significantly higher in the first trimester of pregnancy than at term, and expression in the trophoblast was significantly reduced in placentas from women with fetal growth restriction (FGR). (manchester.ac.uk)
In our study, a 480bp fragment of the capsid protein gene of potato virus Y (CP-PVY) was used as a target to downregulate PVY mRNA expression in-vitro, as the CP gene interferes with viral uncoating, translation and replication. (virosin.org)
Transgenic retina-specific expression of AD5 reveals that stable AD5 protein is initially localized in the nucleus but later found in the cytoplasm concurrent with progressing rod outer segment degeneration and apoptosis. (uni-wuerzburg.de)
We show that small interfering RNA inhibition of SET8 expression leads to decreased cell proliferation and accumulation of cells in S phase. (rupress.org)
Equally unclear is usually whether the EGS RNAs are effective in blocking HCMV gene expression and replication. (azd1152.com)
Scope includes mutations and abnormal protein expression. (cancerindex.org)

Many HEAT binding motifs are found on HTT implying it has roles as a scaffolding protein and is involved in many protein interactions [8]. (fortuneonline.org)

Autophagy is a highly conserved and lysosome-dependent bulk degradation process. (hindawi.com)
Autophagy is the "self-eating" process of degrading damaged proteins and organelles, as well as recycling intracellular energy to maintain cellular homeostasis under stress conditions. (hindawi.com)
Although central to autophagy, targeted proteins have also a high number of connections with proteins of other cellular functions. (sfr-biosciences.fr)
Interestingly, immunity-associated GTPase family M (IRGM), the most targeted protein, was found to interact with the autophagy-associated proteins ATG5, ATG10, MAP1CL3C and SH3GLB1. (sfr-biosciences.fr)
Inhibition of autophagy by pharmacological inhibitors and RNA disturbance reduced trojan replication indicating a significant function for autophagy in NDV an infection. (mdm-inhibitors.com)
Autophagy is usually a highly conserved process that generates double-membrane vesicles that engulf and sequester portions of the cytoplasm to be delivered to the lysosome for degradation (6 7 SL 0101-1 Autophagy is usually induced in response to diverse stress stimuli including nutrient starvation endoplasmic reticulum (ER) stress oxidative stress pathogen-associated molecular patterns (PAMPs) and computer virus infection (6). (mdm-inhibitors.com)
Several autophagy-related proteins have been implicated in the formation of autophagosomes. (mdm-inhibitors.com)

RPA, replication protein A. (rupress.org)
This is accompanied by DNA double-strand break (DSB) induction and recruitment of the DNA repair proteins replication protein A, Rad51, and 53BP1 to damaged regions. (rupress.org)
Furthermore, we find that SET8 interacts with proliferating cell nuclear antigen through a conserved motif, and SET8 is required for DNA replication fork progression. (rupress.org)

Background: The putative functions of the cellular prion protein (PrP c ) are believed to be associated with cell signaling, differentiation, survival, and cancer progression. (iiarjournals.org)

2004) Role of Arabidopsis ARGONAUTE4 in RNA-directed DNA methylation triggered by inverted repeats. (ttu.edu)

Small interfering RNA depletion of Bod1 in HeLa cells produces elongated mitotic spindles with severe biorientation defects. (rupress.org)

The conserved p.Arg108 residue in S1PR2 (DFNB68) is fundamental for proper hearing: evidence from a consanguineous Iranian family. (omicsdi.org)
Likely via a radical-pair mechanism 12 , T 1 reacts within microseconds to the signaling state, characterized by a covalent thioadduct between a highly conserved cysteine residue in the LOV photosensor and the C4a atom of the flavin isoalloxazine ring system. (nature.com)
A highly conserved glutamine residue in strand Iβ is situated immediately adjacent to the flavin and has been identified as instrumental in reading out the flavin N5 position and eliciting the downstream transitions. (nature.com)

Research in my laboratory is directed to understand how multiple small RNA pathways operate in plants using genetic, genomic, and biochemical approaches. (ttu.edu)
Xie Z , and Qi X. (2008) Diverse small RNA-directed silencing pathways in plants. (ttu.edu)
What pathways are this gene/protein implicaed in? (cancerindex.org)

Each class of small RNAs is synthesized by a distinct mechanism and each has discrete biological functions. (biomedcentral.com)
The long term goal of our research is to elucidate the molecular mechanisms by which proteins are targeted to specific and distinct compartments. (stanford.edu)

Finally, codepletion of Rad51, an important homologous recombination repair protein, abrogates the DNA damage after SET8 depletion. (rupress.org)

The discovery of the elaborate and versatile systems of RNA silencing in eukaryotes is one of the pivotal advances in biology of the last decade [ 1 - 6 ]. (biomedcentral.com)

Interestingly, unlike many animals, plants encode multiple DCL and RDR proteins. (ttu.edu)

This suggests that Schlafen proteins might have a conserved role in immunity across various species. (thaimedicalnews.com)

During interphase, several kinetochore proteins stably bind to NPCs (e.g. (nih.gov)

Targeted cleavage of mRNA by human RNase P provides a unique approach to inactivate any RNA of known sequence expressed efficiency of the EGS-induced RNase P cleavage as well as its efficacy is required in order to develop EGSs for practical use both as a research tool and as a therapeutic agent for gene-targeting applications. (azd1152.com)

Initially, the disease was referred to as "mystery swine disease" until its cause was determined to be a positive-stranded RNA virus, designated porcine reproductive and respiratory syndrome virus (PRRSV), that together with murine lactate dehydrogenase-elevating virus (LDV), equine arteritis virus, and simian hemorrhagic fever virus, belongs to the family Arteriviridae ( 6 ). (cdc.gov)
PUCH belongs to a family of proteins known as Schlafen proteins, which are also found in mice and humans. (thaimedicalnews.com)
The recent incursions of African swine fever (ASF), a severe, highly contagious, transboundary viral disease that affects members of the Suidae family, in Europe and China have had a catastrophic impact on trade and pig production, with serious implications for global food security. (mdpi.com)
The site is also conserved in Ago1 and Ago4, but the homology surrounding the site is low between Ago2 and these two family members. (ecmbio.com)
Much of our current work concerns the RanBP2 complex, which consists of RanBP2 (a large nucleoporin that is also known as Nup358), SUMO-1-conjugated RanGAP1 (the activating protein for the Ran GTPase), and Ubc9 (the conjugating enzyme for the SUMO family of ubiquitin-like modifiers). (nih.gov)
The asymmetrical distribution of Ran-GTP and Ran-GDP drives cargo transport between the nucleus and cytoplasm through karyopherins, a family of nuclear transport carrier proteins that bind to Ran-GTP. (nih.gov)

An anaplastic, highly malignant, and usually bronchogenic carcinoma composed of small ovoid cells with scanty neoplasm. (lookformedical.com)

However, it has been proven that more than 62% of genomic DNA serves as a template for transcription, which indicates that there are abundant non-coding RNAs (ncRNAs) in human transcriptome [ 2 , 3 ]. (ijbs.com)

Here we assessed three candidate proteins with the potential to be used as biomarkers in biological fluids: two glucuronidases from E. coli (GusA) and Staphylococcus sp. (uni-wuerzburg.de)

Anatomy16

Organisms21

Diseases1

Chemicals and Drugs109

Analytical, Diagnostic and Therapeutic Techniques and Equipment30

Phenomena and Processes84

Disciplines and Occupations1

Information Science4

Health Care1

SiRNAs6

SiRNA6

Interference8

Eukaryotic8

POLYMERASE6

Evolutionarily2

MiRNAs7

Genes14

PiRNAs3

Genome7

Viruses8

Single-stranded1

MicroRNA3

Transcriptional5

Nucleotides2

Molecules1

Sequences5

Vertebrate1

Functional8

Mammalian cells1

Residues3

Phosphorylation6

Processes4

ARGONAUTE3

Initiation2

Yeast1

Gene Expression Profiling1

Pathway4

Chromosome2

Mitosis1

Variants3

Complexes3

Precursors1

Synthesis1

Biogenesis3

Expression15

Roles1

Autophagy7

Replication3

Cellular1

Methylation1

HeLa1

Residue3

Pathways3

Distinct2

Depletion1

Eukaryotes1

Encode1

Immunity1

Stably1

Cleavage1

Family6

Malignant1

Abundant1

Biomarkers1