• SeeBlue Plus2 Pre-Stained Standard contains 10 proteins (4-250 kDa): 8 blue-dyed and 2 with contrasting colors, for easy and quick evaluation of electrophoresis and western transfer efficiency. (thermofisher.com)
  • Utilizing the same chemistry as black and white photography, silver staining is another highly sensitive method for the visualization of protein bands on electrophoresis gels. (nationaldiagnostics.com)
  • Control of the Staining Procedure after Paper Electrophoresis. (karger.com)
  • Use SYPRO® Ruby Protein Gel Stain for the visualization of proteins in both non-denaturing and denaturing gels, including 2-D electrophoresis. (bio-rad.com)
  • Staining is a critical step in the electrophoresis process. (amerigoscientific.com)
  • Whether it is for protein electrophoresis or western blot, our pre-stained protein markers help you quickly determine the molecular weight of the target protein or evaluate the transfer efficiency. (acrobiosystems.com)
  • Fluorescent Stains: We offer fluorescent SYPRO and Lucy stains for protein electrophoresis. (sigmaaldrich.com)
  • Common artifacts in silver stained gels are bands of keratin in the ranges of 54-57 kDa and 65-68 kDa as a contamination of the sample prior to the electrophoresis. (wikipedia.org)
  • Coomassie Blue staining: Staining of protein gels with Coomassie Brilliant Blue R-250 is a common procedure to visualize proteins resolved by SDS-PAGE. (sigmaaldrich.com)
  • We offer a highly sensitive protein detection silver stain kit suitable for SDS-PAGE gels. (sigmaaldrich.com)
  • Gels to be stained are fixed with ethanol/acetic acid solution prior to staining with 100X Speedy Protein Gel Stain Kit. (fairbiotech.com)
  • Gels stained with 100× Speedy Protein Gel Stain Dye may be directly visualized with a variety of different UV-based fluorescence imaging systems. (fairbiotech.com)
  • Silver staining was introduced by Kerenyi and Gallyas as a sensitive procedure to detect trace amounts of proteins in gels. (wikipedia.org)
  • citation needed] Silver staining is used to stain gels. (wikipedia.org)
  • The silver stain of proteins in Agarose gels was developed in 1973 by Kerenyi and Gallyas. (wikipedia.org)
  • Later it was adapted to polyacrylamide gels used in SDS-PAGE, and also for staining DNA or RNA. (wikipedia.org)
  • Gels can remain in the stain for weeks without concern. (selectscience.net)
  • Thus, users are advised to fully validate their application if they desire to obtain high quality data that includes surface proteins, intracellular proteins, and RNA expression data. (biolegend.com)
  • This includes TotalSeq™ antibodies targeting intracellular proteins of interest. (biolegend.com)
  • ELISA-ONE are next generation ready to use ELISA kits for the fast detection of key intracellular proteins that undergo phosphorylation in major signaling pathways. (selectscience.net)
  • Here we used an affinity-purification mass spectrometry-based (AP-MS) approach to identify novel and particularly intracellular sGAG-interacting proteins in human bone marrow stromal cells (hBMSC). (degruyter.com)
  • SYPRO® Ruby Protein Gel Stain is highly sensitive, simple to use fluorescent stain that can accurately quantitate protein expression levels, and is compatible with standard fluorescent visualization systems and downstream identification techniques such as mass spectrometry. (lonza.com)
  • The bound 100× Speedy Protein Gel Stain Dye is easily removed from the protein by immersing the gel in sufficient water, thus it is well compatible with subsequent enzymatic digestion and mass spectrometry for proteomics applications. (fairbiotech.com)
  • Protein identity confirmed by mass spectrometry. (rndsystems.com)
  • Proteins stained using the Coomassie ProStain ™ are compatible with mass spectrometry (MS) analysis. (amidbiosciences.com)
  • The following protocol describes combined surface and intracellular protein staining with TotalSeq™-B antibodies, with or without TotalSeq™-B hashtag antibodies using the Single Cell 3' v3.1 Feature Barcoding technology from 10x Genomics. (biolegend.com)
  • This protocol has been validated solely for the detection of surface or intracellular protein expression with the corresponding TotalSeq™-B antibodies. (biolegend.com)
  • Our liquid, single-antibody TotalSeq™ conjugates require optimization of staining concentration (titration) to obtain best results, as performed by the end user of the antibodies. (biolegend.com)
  • Optimization of antibody staining concentration is essential to obtain good quality data in antibody-based applications such as surface and intracellular staining using TotalSeq™ antibodies. (biolegend.com)
  • Enzymatic digestion may result in alterations of surface protein epitopes and impact staining with TotalSeq™ antibodies. (biolegend.com)
  • If a knowledge-based annotated protein expression is provided, this is reported for each cell type at the top of the page and the staining level of the individual antibodies is given underneath each antibody ID. (proteinatlas.org)
  • From the enlarged view, all stained images for all antibodies can be browsed (represented by miniature images). (proteinatlas.org)
  • Abcam: antibodies, proteins, kits. (abcam.com)
  • Monoclonal antibodies (mAbs) to methylene diphenyl diisocyanate (MDI) and toluene diisocyanate (TDI) conjugated protein: research and potential biomonitoring applications. (cdc.gov)
  • The 100X Speedy Protein Gel Stain is a fast and sensitive fluorescent dye for visualization and quantitation of proteins separated by 1-D or 2-D SDS-PAGE. (fairbiotech.com)
  • Silver staining aids the visualization of targets of interest, namely intracellular and extracellular cellular components such as DNA and proteins, such as type III collagen and reticulin fibres by the deposition of metallic silver particles on the targets of interest. (wikipedia.org)
  • This stain is recommended for rapid and reversible protein staining on nitrocellulose or PVDF membranes. (alfa-chemistry.com)
  • To a volume of protein sample (cell or tissue lysate), add equal volume of loading buffer. (sigmaaldrich.com)
  • The detailed normal tissue page shows images of the stained tissue, together with antibody staining/expression level of the cell types. (proteinatlas.org)
  • In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (rndsystems.com)
  • immunochemical staining of dermal tissue and enzyme-linked immunoassays (ELISA). (cdc.gov)
  • If using cells isolated with a different procedure, users may need to verify the antibody staining pattern using alternative methods. (biolegend.com)
  • 544-MBS212521 Product Name DAXX, Monoclonal Antibody Host Mouse Specificity Mouse anti Human DAXX antibody, clone DAXX-01 recognizes human Death domain-associated protein 6, also known as DAXX, ETS1-associated. (bioscience-explained.org)
  • 639-abx010472 Product Name Brain Natriuretic Peptide (BNP), Monoclonal Antibody Clonality Monoclonal Isotype IgG2b Kappa Host Mouse Species Reactivity Mus musculus (Mouse) Purity/Purification Protein A + Protein G. (bioscience-explained.org)
  • 513-CABT-B1265 Species reactivity Human Host/Isotype Mouse / IgG1 To classify Monoclonal Type Antibody Clone OTI7B10 Immunogen Full length human PDCD1LG2 recombinant protein produced in HEK293T cell. (bioscience-explained.org)
  • Samples from up to three different individuals have been stained for each antibody. (proteinatlas.org)
  • The protein expression bar, with the units none (n), low (l), medium (m) and high (h), is based on a best estimate of the "true" protein expression for proteins where the antibody staining pattern has been analyzed by knowledge-based annotation. (proteinatlas.org)
  • Highly sensitive and specific recombinant antibody microarrays allowing screening of crude protein extracts are also being developed [ 14 ]. (hindawi.com)
  • Considering the level of desired protein sensitivity and the intended downstream analysis, Amerigo Scientific offers a variety of ready-to-use protein staining reagents for your need. (amerigoscientific.com)
  • clean glassware, pure reagents, and water of highest purity are the key points to successful staining. (wikipedia.org)
  • Furthermore, the cleanliness of the vessels used and the purity of the reagents influence the silver stain. (wikipedia.org)
  • Commonly used staining methods include Coomassie staining, silver staining, and fluorescent staining. (amerigoscientific.com)
  • The 100X Speedy Protein Gel Stain is normally low fluorescent but emits strong fluorescence (bright golden color) as bound to proteins. (fairbiotech.com)
  • Isami, K , Amagai, M & Takahashi, H 2021, ' Simultaneous detection of mouse Foxp3 and cytosolic fluorescent protein by a modified intracellular staining protocol ', Journal of Dermatological Science , vol. 101, no. 2, pp. 147-149. (elsevierpure.com)
  • This stain has a higher sensitivity than many other protein stains, ideal for detection of difficult-to-stain proteins such as glycoproteins and lipoproteins. (bio-rad.com)
  • Here, we develop a biological nanopore sensor for single-molecule detection of proteins and PDIs using the pore-forming toxin YaxAB. (nature.com)
  • Using this YaxAB nanopore, we demonstrate label-free, single-molecule detection of interactions between the anticancer Bcl-xL protein and small-molecule drugs as well as the Bak-BH3 peptide. (nature.com)
  • It gives exceptional sensitivity and wide dynamic range for protein detection. (fairbiotech.com)
  • To make the Coomassie Blue G-250 staining reagent, dissolve 0.2g dye in 100 ml H 2 O (this will require warming to approximately 50°C). Cool and add 100 ml 2N H 2 S0 4 . (nationaldiagnostics.com)
  • Stain gel in 10% acetic acid in water, containing 60 mg/L of Coomassie Blue R-250 . (nationaldiagnostics.com)
  • First, the proteins are denatured in the gel by a fixative solution of 10% acetic acid and 30% ethanol and precipitated, at the same time the detergent (mostly SDS) is extracted. (wikipedia.org)
  • Unlike traditional Coomassie ® stains, Coomassie ProStain ™ does not require fixatives and destains containing organic solvents (methanol or ethanol) or acetic acid. (amidbiosciences.com)
  • Ponceaus S Protein Staining Solution is a ready-to-use membrane stain for evaluating the transfer efficiency of a western blot. (alfa-chemistry.com)
  • 1. After the SDS-PAGE Gel has been transferred to a nitrocellulose or PVDF membrane, place the transferred membrane in a staining container. (alfa-chemistry.com)
  • 2. Submerge the membrane in a proper amount of Ponceau S Protein Staining Solution that is enough to cover the whole membrane. (alfa-chemistry.com)
  • 3. Wash away the excessive stain on the membrane with DI water until the protein bands are clear. (alfa-chemistry.com)
  • 4. For the further membrane blocking step, remove the Ponceau S Staining Solution from the membrane by placing the membrane in 20 ml 0.1% NaOH for 1 to 2 minutes then rinse in DI water for 1 to 2 minutes. (alfa-chemistry.com)
  • Ponceau S solution can be used to evaluate for total protein amount or transfer efficiency on nitrocellulose and PVDF membrane. (alfa-chemistry.com)
  • The PVDF membrane stained Ponceau S for 5 mins and washed with ddH2O for 3 mins. (alfa-chemistry.com)
  • Jones' stain, a methenamine silver-Periodic acid-Schiff that stains for basement membrane, availing to view the "spiked" GBM associated with membranous glomerulonephritis. (wikipedia.org)
  • RBC membrane proteins separated by SDS-PAGE and silver-stained. (wikipedia.org)
  • Ribosomal proteins (RPs) play important roles in modulating the MDM2-p53 pathway. (elifesciences.org)
  • Stain gel in the above solution, with 0.25% Coomassie Blue R-250 , for 2 - 4 hours, until the gel is a uniform blue color. (nationaldiagnostics.com)
  • 95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. (rndsystems.com)
  • 2 μg/lane of Biotinylated Recombinant Human ACE-2 Fc Chimera Avi-tag Protein (Catalog # AVI10544) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at ~124 kDa under reducing conditions. (rndsystems.com)
  • Though less sensitive, Coomassie G-250 can be used in place of the R-250 form to create a rapid and convenient staining procedure. (nationaldiagnostics.com)
  • The staining procedure is a simple two-step protocol (fix and stain) that can be completed in as little as 30 minutes. (fairbiotech.com)
  • imaging sensitivity might be moderately enhanced after 4°C storage of the stained gel. (fairbiotech.com)
  • silver staining increases the sensitivity typically 50 times. (wikipedia.org)
  • Silver ions are reduced to insoluble silver metal granules in the vicinity of the protein molecules. (nationaldiagnostics.com)
  • Staining appears to be dependent upon the initial reduction and resulting immobilization of a small number of silver ions by the proteins in the gel. (nationaldiagnostics.com)
  • The glycosylations of glycoproteins and polysaccharides can be oxidised by a 1-hour pre-treatment with 0.1% periodic acid at 4 °C, which improves the binding of silver ions and the staining result. (wikipedia.org)
  • Silver ions bind to negatively charged side chains of the proteins. (wikipedia.org)
  • The Coomassie dyes ( R-250 and G-250 ) bind to proteins through ionic interactions between dye sulfonic acid groups and positive protein amine groups as well as through Van der Waals attractions. (nationaldiagnostics.com)
  • Silver nitrate stains the nucleolar organization region (NOR)-associated protein, producing a dark region wherein the silver is deposited and denoting the activity of rRNA genes within the NOR. (wikipedia.org)
  • it still remains to be determined if there are more proteins that can regulate the RPs-MDM2-p53 pathway. (elifesciences.org)
  • Here, we report that the microRNA miR-1 regulates the autophagy pathway through conserved targeting of the orthologous T re-2/ B ub2/ C DC16 (TBC) Rab GTPase-activating proteins TBC-7 and TBC1D15 in Caenorhabditis elegans and mammalian cells, respectively. (elifesciences.org)
  • Pre-formed fibrils are an invaluable preclinical model for exploring pathogenesis of neurological diseases through aggregation of misfolded proteins. (acrobiosystems.com)
  • Therefore, this work provides a potential therapeutic strategy for protein aggregation disorders. (elifesciences.org)
  • Due to the central role of autophagy in the removal of aggregation-prone proteins, a better understanding of mechanisms controlling autophagy is essential for the identification of novel therapeutic opportunities for multiple disease states. (elifesciences.org)
  • This prompted us to investigate if mir-1 is required for preventing the accumulation of aggregation-prone proteins. (elifesciences.org)
  • Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. (rndsystems.com)
  • Lipids, polysaccharides and nucleic acids bind to proteins which increases the viscosity of the samples and often results in horizontal streaking on the 2D-PAGE. (hindawi.com)
  • ACE-2 is the SARS-CoV and SARS-CoV2 Spike protein receptor in vivo (4-6), functions catalytically as a carboxypeptidase to cleave several substrates including angiotensins I and II, and acts as a partner for B0AT1-family amino acid transporters (1, 2). (rndsystems.com)
  • The interaction of sGAG with α2-macroglobulin receptor-associated protein (LRPAP1), exportin-1 (XPO1), and serine protease HTRA1 (HTRA1) was confirmed in reverse assays. (degruyter.com)
  • Reactive oxygen species (ROS) levels were determined using dihydrodichlorofluorescein staining, and the chymotrypsin-like protease activities were measured using the Suc-LLVY-aminoluciferin substrate. (molvis.org)
  • by Abnova - Automated protein synthesizer is an expert system in transcription, translation, and purification of recombinant protein. (dnatube.com)
  • Watch amylase enzymes break down stains from ready-made, processed, and convenience foods like sauces, dressings, and desserts. (novozymes.com)
  • Watch lipase enzymes break down stains from fast foods and other greasy dishes. (novozymes.com)
  • Watch pectinase enzymes break down stains from jams, yogurt, and smoothies. (novozymes.com)
  • Watch mannanase enzymes break down stains from processed and convenience foods and personal care products. (novozymes.com)
  • Proteolytic enzymes can break down proteins, including those in your pellicle layer. (healthline.com)
  • The silver granules which make up the latent image act to catalyze this process, resulting in more rapid deposition of silver at the sites of the protein bands. (nationaldiagnostics.com)
  • Under proper conditions, a gel placed in an acidified solution of Coomassie G-250 will manifest blue protein bands on a light amber background. (nationaldiagnostics.com)
  • Protein bands will be stained and start becoming visible in 5 to 15 minutes. (alfa-chemistry.com)
  • Only the proteins are stained, resulting in extremely well defined blue bands on a highly tr. (selectscience.net)
  • In this protocol, background staining is low due to the very low dye concentration used. (nationaldiagnostics.com)
  • Clones not validated using this protocol should first be tested using this protocol to ensure staining is valid. (biolegend.com)
  • In order to gain the best possible protein separation on 2D-PAGE, an optimized sample preparation protocol for placental proteins was developed. (hindawi.com)
  • Intracellular Staining Permeabilization Wash Buffer (10x) (BioLegend, Cat. (biolegend.com)
  • Cell Staining Buffer (BioLegend, Cat. (biolegend.com)
  • After the staining step, wash the gel several times with distilled water to remove excess stain. (sigmaaldrich.com)
  • For stains on carpeting, use a spoon to pick up the excess, working from the edge of the stain to the center. (doityourself.com)
  • Staining of intracellular molecules can sometimes require specific fixation and permeabilization conditions that are not conducive between all clones. (biolegend.com)
  • Neural factors are a class of protein molecules with neurotrophic activity that can promote the survival and regeneration of nerve cells. (acrobiosystems.com)
  • Explore our comprehensive collection of recombinant Fc receptor proteins! (acrobiosystems.com)
  • 19 normal healthy in- receptor protein (CD117) that is structurally dividuals were recruited as a control group. (who.int)
  • Protein markers are an essential component in biological research and drug development. (acrobiosystems.com)
  • others are merely artifacts of fixation and staining. (bvsalud.org)
  • Versatile - Actichem Bust removes more than just protein stains. (actichem.com.au)
  • In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere. (rndsystems.com)
  • The Ponceau S Protein Staining Solution is provided as a ready-to-use solution, there is no need to dilute before use. (alfa-chemistry.com)
  • While the gel is washing, prepare a staining solution (see below). (nationaldiagnostics.com)
  • Staining is complete when the gel is no longer visible in the dye solution. (nationaldiagnostics.com)
  • Prior to complete staining, the gel will appear as a lighter area against the dark staining solution. (nationaldiagnostics.com)
  • Staining solution is stable for 2 - 3 weeks @ 25°C. (nationaldiagnostics.com)
  • Filter the stain solution using Whatmann No. 1 filter paper. (sigmaaldrich.com)
  • Radical Chemical Action - Actichem Bust employs powerful protein dissolving chemistry for a reliable solution where other cleaners have failed. (actichem.com.au)
  • These reduce silver solution to metallic silver after being exposed to the stain that contains a reductant, for example hydroquinone or formalin. (wikipedia.org)
  • This stain eliminates extensive solution preparation time and expenditure. (amidbiosciences.com)
  • If the stain remains, mix a solution of one tablespoon grease cutting dish soap, like Dawn, with one quart of water. (doityourself.com)
  • The generated proteins display foldings and biological functions similar to the mammalian counterparts. (dnatube.com)
  • The protein concentration range in CSF as well as glucose track can also change according to age. (bvsalud.org)
  • CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. (rndsystems.com)
  • Typically, stains have multiple components. (novozymes.com)
  • Typically in CSF, levels of protein increase, and because bacteria consume glucose and because less glucose is transported into the CSF, glucose levels decrease. (msdmanuals.com)
  • The "self"-protein albumin in mouse airway fluid was uniquely modified by GSH-MDI at position 414K, a preferred site of MDI reactivity on human albumin. (cdc.gov)
  • 372-CA006-100 Target Protein Cytomegalovirus ICP36 DNA Binding Protein Target Protein Also Known As pUL44, MDBP, pp52, DB52 Clone Designation 10D8 Species/Isotype Mouse IgG1(k) Purity Affinity. (bioscience-explained.org)
  • Streptavidin is a tetrameric protein providing 4 high-affinity biotin binding sites. (acrobiosystems.com)
  • Affinity constants for the TDI mAb are very high (108-1010 M-1) suggesting greater utility for immunoassay and immunohistochemistry, but limited use for immunoprecipitation/protein isolation. (cdc.gov)
  • Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune co-stimulatory receptors. (rndsystems.com)
  • Defective autophagy results in accumulation of toxic protein aggregates that detrimentally affect cellular function and organismal survival. (elifesciences.org)
  • It is inherited as an autosomal dominant syndrome as a result of defective mismatch repair (MMR) proteins. (medscape.com)
  • By denaturing the proteins and giving them a uniform negative charge, it is possible to separate them based on the size as they migrate towards the positive electrode. (sigmaaldrich.com)
  • Explore our series of high-quality proteins covering comprehensive diagnostic indicators in order to facilitate the in vitro diagnostic research of neurological diseases. (acrobiosystems.com)
  • This high throughput system produces recombinant proteins in vitro. (dnatube.com)
  • Although protein-protein interactions (PPIs) are promising targets, drugging them is one of the key challenges in drug discovery 19 . (nature.com)
  • Glycosaminoglycans (GAGs) are multifunctional polysaccharides of the extracellular matrix (ECM) responsible for ECM hydration and binding of cations and proteins due to their negative charge. (degruyter.com)
  • Actichem Bust is a radical strength protein and blood removal formulation which also produces outstanding results on cleaning tough soiling from nylon carpeting. (actichem.com.au)
  • A hybridoma that produces MDI-protein specific IgM mAbs, which also has good cross-reactivity to hexamethylene diisocyanate conjugated protein, has recently been isolated and cloned. (cdc.gov)
  • Effort's to create a hybridoma that produces an anti- MDI-protein IgG are ongoing. (cdc.gov)