A "subunit" vaccine doesn't contain the whole pathogen, unlike live attenuated or inactivated vaccine, but contains only the antigenic parts such as proteins, polysaccharides or peptides. (wikipedia.org)
Antigen5
Nanoparticles (NPs) for allergen immunotherapy have garnered attention for their high efficiency and safety compared with naked antigen proteins. (bvsalud.org)
In this work, we present mannan-coated protein NPs, incorporating antigen proteins for antigen-specific tolerance induction. (bvsalud.org)
Here, the NPs were formed spontaneously via heat denaturation of three component proteins: an antigen protein, human serum albumin (HSA) as a matrix protein, and mannoprotein (MAN) as a targeting ligand for dendritic cells (DCs). (bvsalud.org)
We applied this method to various antigen proteins and found that the self-disperse after heat denaturation was a requirement for incorporation into the NPs. (bvsalud.org)
The Avidin-Biotin system has been utilized extensively for enzyme-linked immuno solid-phase assay (ELISA), in which an enzyme-Avidin conjugate (useful for detection by reaction with the enzyme's substrate to afford a colored or chemiluminescent product) is employed to detect the presence of a biotinylated antibody, after first binding the antibody to an immobilized antigen or hapten. (justia.com)
Antibody4
Preparation of Hapten-carrier Immunogen conjugates - antibody modification and conjugation immunotoxin conjugation techniques - liposome conjugated and derivatives- Colloidal - gold- labeled proteins - modification with synthetic polymers. (notesforgeeks.in)
There are many varieties of antibody conjugation technologies available and each has its benefits, though no single one is optimal for every payload and antibody. (stratech.co.uk)
This antibody and its recombinant constructs were able to recognize proteins of Loxosceles spider venoms with specificity. (bvsalud.org)
Biotin2
Properties of common enzymes - Activated enzymes for conjugation - biotinylated enzymes - chemical modification of nucleic acids - biotin labeling of DNA- enzyme conjugation to DNA - Fluorescent of DNA. (notesforgeeks.in)
Although numerous methods of indirect bioconjugate preparation have been described, a significant number of those reported in the literature have been prepared by exploiting the Avidin-Biotin system, in which, the binding specificity of the protein Avidin (purified from egg white), or Streptavidin (purified from the bacterium Streptomyces avidinii), toward the cofactor Biotin (vitamin H) is utilized to bridge an Avidin conjugated macromolecule with a biotinylated macromolecule. (justia.com)
Molecules2
A protein subunit is a polypeptide chain or protein molecule that assembles (or "coassembles") with other protein molecules to form a protein complex. (wikipedia.org)
We specialize in highly modified siRNA, including conjugation to molecules such as cholesterol for cellular delivery. (bocsci.com)
Probes2
Disclosed are materials and methods for performing multiplex assays for nucleic acids, in which a transponder is associated with the bead(s) forming the solid phase used in the assay, nucleic acid probes are bound to the surface of the particles, and data concerning the assay is encoded on the transponder. (justia.com)
This invention relates to materials and methods for detecting nucleic acids in samples and, more particularly, to solid phase assays wherein transponders are associated with the beads constituting the solid phase, nucleic acid probes are bound to the surface of the particles and data concerning the assay is encoded on the transponders. (justia.com)
Fluorescent1
iFluor™ superior fluorescent labelling dyes, optimised for labelling proteins and nucleic acids. (stratech.co.uk)
Assay3
Screen Quest™ assay kits, a set of HTS-ready tools for high throughput screening of biochemical and cellular targets such as protein kinases, proteases, HDAC, cell apoptosis and cytoxicity, GPCR, ion channels, ADME/metabolism and transporters. (stratech.co.uk)
In an electronically-indexed multiplex assay of this invention, two or more classes of transponders, each encoded with a different index number and constructed to bind a different nucleic acid sequence, are incubated with the sample in a single vessel. (justia.com)
Protein production was monitored by fluorescence assay. (glenresearch.com)
Fluorescence1
After necessary washes, incubations and additions are performed, the solid phase is analyzed to detect a label indicative of binding of nucleic acid in the sample to the oligonucleotide on the transponder, such as fluorescence, color, radioactivity or the like. (justia.com)
Synthesis1
We will support your cholesterol-siRNA conjugation research with our expertise in high quality modified nucleic acid synthesis and excellent technical support. (bocsci.com)
Recombinant3
Basic components of recombinant subunit vaccines include recombinant subunits, adjuvants and carriers. (wikipedia.org)
A key step in creating a recombinant protein vaccine is the identification and isolation of a protein subunit from the pathogen which is likely to trigger a strong and effective immune response, without including the parts of the virus or bacterium that enable the pathogen to reproduce. (wikipedia.org)
Protein subunit vaccines are generally made through protein production, manipulating the gene expression of an organism so that it expresses large amounts of a recombinant gene. (wikipedia.org)
Preparation2
The heat-induced formation of protein NPs is a one-pot preparation method and can be applied to various proteins. (bvsalud.org)
The present invention relates to the field of bioconjugate preparation, and more particularly, to a class of bifunctional boronic compound complexing reagents useful for the conjugation of biological macromolecules, and the method of making and using such reagents. (justia.com)
Cholesterol1
Improved transport through the cellular membrane can be attained by use of a carrier molecule (e.g., cholesterol, poly-lysine) or by backbone modification to more lipophilic linkages. (glenresearch.com)
Suitable2
Parts of the protein shell or capsid of a virus are often suitable. (wikipedia.org)
HSA is non-immunogenic, therefore suitable as a matrix protein, while MAN coats the surface of the NP. (bvsalud.org)
Cell1
We decellularized the metastatic lung sections, measured the micromechanics of the tumor ECM, and stained the sections for ECM proteins, proliferation, and cell death markers. (ibecbarcelona.eu)
Solid2
a sensitive and robust tool for detection of proteins in solutions and solid surfaces (such as gels and membranes). (stratech.co.uk)
Solid phase assays have been used to determine the presence of nucleic acids, including deoxyribonucleic acids (DNA), ribonucleic acids (RNA) and their modified forms. (justia.com)
Small1
Large assemblies of proteins such as viruses often use a small number of types of protein subunits as building blocks. (wikipedia.org)
Production1
Antisense inhibition of the RNA target sequence would arrest the production of one protein without affecting the expression of the second control protein. (glenresearch.com)
Peptides3
It contains numerous sites per molecule for effective conjugation of peptides and other antigens using amine-reactive or carboxyl-reactive crosslinkers. (aatbio.com)
The carboxyl-reactive carbodiimide reacts with exposed carboxyl and amino groups on peptides and proteins to form stable bonds. (aatbio.com)
A "subunit" vaccine doesn't contain the whole pathogen, unlike live attenuated or inactivated vaccine, but contains only the antigenic parts such as proteins, polysaccharides or peptides. (wikipedia.org)
Antibody4
Consequently, BSA is a popular carrier protein for conjugation to haptens and other weak antigens to make them more immunogenic for antibody production. (aatbio.com)
This ReadiLink™ BSA Conjugation kit is primarily optimized for the simple preparation of hapten-carrier conjugates for immunization and antibody production. (aatbio.com)
The resulting conjugate is used for eliciting an immune response and antibody production against the hapten. (aatbio.com)
They named these proteins "fodrin" as antibody studies showed that they were localized in the sheath under the axonal membrane, but not in the core of the axon. (biocheminfo.org)
Adjuvants1
For example, ficoll has been intensively studied as a carrier for CpG adjuvants, which has been shown in adjuvants to include size (a key determinant of delivery to the phagocytic system of macrophages), high water solubility, capacity for a variety of surface chemistries, and the expected advantages such as immunogenicity and toxicity and ease of lyophilization. (cd-bioparticles.net)
Immune response2
Furthermore, a dendritic cell targeting head mannose monomer and a mevalonate pathway inhibitor zoledronic acid (Zol) were integrated or absorbed onto the nanoparticles (denoted as MEAO-Z) to intensify the immune response. (bvsalud.org)
Subunit vaccines contain fragments of the pathogen, such as protein or polysaccharide, whose combinations are carefully selected to induce a strong and effective immune response. (wikipedia.org)
Molecular biology1
He is trained in the fields of protein engineering, molecular biology and structural biology at University of Toronto and Stanford University and Queen's University. (chi-peptalk.com)
Complex1
In addition, the 67 kDa protein is sufficiently large and complex to be fully immunogenic. (aatbio.com)
Components1
The spectrin family of proteins were originally discovered as major components of the submembraneous cytoskeleton of osmotically lyzed red blood cells. (biocheminfo.org)
His research focuses on the applications of NMR in the higher-order structural characterization of antibodies and fusion proteins. (chi-peptalk.com)
Drug2
However, most nanovaccines fabricated with nanomaterials suffer from carrier-related concerns, including low drug loading capacity, unpredictable metabolism, and potential systemic toxicity, which bring obstacles for their clinical translation. (bvsalud.org)
He obtained his PhD in Biochemistry from Northwestern University in 2008 where he studied protein structure-based drug design of novel chemotherapeutic agents. (chi-peptalk.com)
Include1
Subsequently, he worked as a postdoctoral researcher in the group of Prof. T.P.J. Knowles at the Department of Chemistry at the University of Cambridge, U.K. His research interests include the development, characterization and formulation of therapeutic proteins. (chi-peptalk.com)
Step2
The ReadiLink™ BSA Conjugation kit is one-step conjugation of a hapten to a carrier protein using the carboxyl-reactive carbodiimide as the crosslinker. (aatbio.com)
These kits contain BSA formulated in buffers compatible with the carboxyl-reactive carbodiimide reactions and desalt spin columns, which offer exceptional protein recovery by simple centrifugation step. (aatbio.com)