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  • elution
  • Using an automatic purifier, like an AKTA, a gradient elution can be done to help resolve the protein of interest from contaminating host cell proteins that bind with slightly different affinities so the smaller bead size does hold some value if you've got the tools to make use of it and need the better resolution. (protocol-online.org)
  • Because of the abundant separating columns, elution systems, and detectors available, chromatography has developed into the main method for ion analysis. (wikipedia.org)
  • 1975
  • Not until 1975 was "ion chromatography" established as a name in reference to the techniques, and was thereafter used as a name for marketing purposes. (wikipedia.org)
  • Methods
  • This methods also utilizes a separation gel made of an acrylamide-pendent Phos-Tag monomer that is copolymerized. (wikipedia.org)
  • In contrast to chemical biotinylation methods, enzymatic biotinylation allows biotin to be linked at exactly one residue present in the protein. (wikipedia.org)
  • analyze
  • The eastern blot is a biochemical technique used to analyze protein post translational modifications (PTM) such as lipids, phosphomoieties and glycoconjugates. (wikipedia.org)
  • genetic
  • The Plasma Proteins: Structure, Function and Genetic Control. (wikipedia.org)
  • A 285 amino acid segment of the Acetobacter xylinum cellulose synthase containing all the conserved residues in the globular region was subjected to protein modeling using the genetic algorithm. (embl-heidelberg.de)
  • The genetic code of protein C inhibitor is similar to alpha 1-antitrypsin and alpha 1-antichymotrypsin. (wikipedia.org)
  • probes
  • Transferred proteins are analyzed for post-translational modifications using probes that may detect lipids, carbohydrate, phosphorylation or any other protein modification. (wikipedia.org)
  • bacterial
  • Protein C inhibitor a potent antimicrobial agent that have ability to destroy the bacterial cell wall, causing death of the bacteria. (wikipedia.org)
  • Enzymatic biotinylation can be carried out in vitro but BirA also reacts specifically with its target peptide inside mammalian and bacterial cells and at the cell surface, while other cellular proteins are not modified. (wikipedia.org)
  • Expression
  • Enzymatic biotinylation can also take place in vivo typically through the co-expression of an Avitag tagged protein and BirA. (wikipedia.org)
  • matrix
  • therefore, ion chromatography may have higher matrix tolerance. (wikipedia.org)
  • Cells embedded in agarose on a microscope slide are lysed with detergent and high salt to form nucleoids containing supercoiled loops of DNA linked to the nuclear matrix. (wikipedia.org)
  • All proteins, RNA, membranes and cytoplasmic and nucleoplasmic constituents are disrupted and diffuse into the agarose matrix. (wikipedia.org)
  • The concept underlying the SCGE assay is that undamaged DNA retains a highly organized association with matrix proteins in the nucleus. (wikipedia.org)