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  • highly conserved
  • It is a highly conserved protein in eukaryotes, with a fundamental role in selective protein degradation by the ubiquitin-proteasome system (UPS). (biologists.org)
  • Hereditary inclusion body myopathy associated with early-onset Paget disease of bone and frontotemporal dementia (hIBMPFTD) is a degenerative disorder caused by single substitutions in highly conserved residues of p97/VCP. (asm.org)
  • The ubiquitous v alosin- c ontaining p rotein (p97/VCP) is a prominent member of the highly conserved AAA + ( A TPases a ssociated with diverse cellular a ctivities) proteins, which are known for their oligomeric structure and chaperone-like activities. (asm.org)
  • The pathogenesis of hIBMPFTD is attributed to autosomal-dominant single-amino-acid substitutions in highly conserved residues within the p97 NH 2 domain and D1 ring ( 28 ). (asm.org)
  • specificity
  • The interaction of residues Leu292 of TAXI-IA and Pro294 of TAXI-IIA with the −2 glycon subsite of the xylanase is shown to be critical for both inhibition strength and specificity. (wiley.com)
  • New designed repeat proteins with binding specificity for serum albumin are described, as well as nucleic acids encoding such serum albumin binding proteins, pharmaceutical compositions comprising such proteins, the use of such proteins to modify the pharmacokinetics of therapeutic relevant polypeptides and the use of such proteins in the treatment of diseases. (patents.com)
  • Examples of such recombinant binding proteins are proteins which comprise an ankyrin repeat domain with the desired binding specificity, and a polyethylene glycol moiety. (patentgenius.com)
  • The approach has enabled modulation of enzyme specificity and identification of the functional roles of distinct residues and regions. (nii.ac.jp)
  • By applying the statistical coupling analysis to the study of the three sub-families, it was possible to obtain a detailed depiction of their conserved and correlated characteristics, relating them to the accumulated knowledge on protein tyrosine phosphatases and open questions such as protein regulation by dimerization, specificity and disease-related mutations. (usp.br)
  • We identified two DSP domain residues that are responsible for SEX4 site-specific activity and, using these insights, we engineered a SEX4 double mutant that completely reversed specificity from the C6 to the C3 position. (pnas.org)
  • compounds
  • Tissue and blood proteins are common compounds on contaminated surgical instruments. (sychem.co.uk)
  • defined bacteriocins as proteinaceous compounds that kill closely related bacteria but it is now recognized that bacteriocins can also have activity across genera (broad spectrum) [ 2 - 4 ]. (hindawi.com)
  • Provided are pH- and/or light-responsive compounds capable of controlling the channel activity of a mechanosensitive channel, such as the MscL channel protein of E. coli , or a functional equivalent thereof, and use of these compounds to convert a mechanosensitive channel protein into a pH- and/or light-responsive channel. (google.com)
  • antibody
  • If you cannot find the target and/or product is not available in our catalog, please click here to contact us and request the product or submit your request for custom elisa kit production , custom recombinant protein production or custom antibody production . (mybiosource.com)
  • distinct
  • Phylogenetic analyses show that the novel Aplysia G-protein coupled receptor-like proteins represent three distinct monophyletic subfamilies. (ufl.edu)
  • biochemical
  • p97/VCP is an essential biochemical component of a wide range of ubiquitin-linked cell biological reactions, including ubiquitin-proteasome system-mediated protein degradation ( 8 ), Golgi and endoplasmic reticulum (ER) membrane fusion ( 1 , 18 ), transcription factor activation ( 19 ), and DNA repair ( 10 , 16 ). (asm.org)
  • Many proteins are enzymes that catalyse biochemical reactions and are vital to metabolism . (wikipedia.org)
  • mammalian
  • Using the CRISPR/Cas9 gene editing tool, the objective of this study was to determine if UBA52 protein is required for mammalian embryogenesis. (biologists.org)
  • interaction
  • This blot-based method depends on specific addition of biotin-conjugated cysteamine to the DHA residue followed by detection of biotinylated protein based on its interaction with streptavidin. (ewha.ac.kr)
  • modification
  • Also described are a method by which these reagents can be prepared as well as a method for using them in hydrated media for the modification of proteins. (google.com)
  • degradation
  • In addition to its role in protein degradation, the non-proteolytic consequences of protein ubiquitination also play an important role in cellular functions such as signaling, cell cycle control, transcriptional regulation and apoptosis ( Komander and Rape, 2012 ). (biologists.org)
  • fluorescence
  • It is common research practice for biologists to introduce a gene (or a gene chimera) encoding an engineered fluorescent protein into living cells and subsequently visualize the location and dynamics of the gene product using fluorescence microscopy. (scholarpedia.org)
  • In a footnote to his paper describing the isolation of the bioluminescent protein aequorin from Aequorea , Shimomura wrote, "A protein giving solutions that look slightly greenish in sunlight though only yellowish under tungsten lights, and exhibiting a very bright, greenish fluorescence in the ultraviolet of a Mineralite [a handheld ultraviolet lamp], has also been isolated from squeezates" (Shimomura et al. (scholarpedia.org)
  • Soon after, Shimomura reported the fluorescence emission spectrum of this protein and suggested that energy transfer from aequorin to this green fluorescent protein could explain why the in vivo luminescence of Aequorea is greenish and not blue like the luminescence of purified aequorin (Johnson et al. (scholarpedia.org)
  • ankyrin
  • Limits of Cooperativity in a Structurally Modular Protein: Response of the Notch Ankyrin Domain to Analogous Alanine Substitutions in Each Repeat, J. Mol. (patentgenius.com)
  • Zahnd et al, A Designed Ankyrin Repeat Protein Evolved to Picomolar Affinity to Her2, J. Mol. (patentgenius.com)
  • novel
  • We then demonstrate that the sensitization is facilitated through a novel mechanism, whereby reducing agents chelate Zn 2+ ions off specific extracellular histidine residues in domain I of Ca v 3.2, thereby relieving tonic channel inhibition. (jneurosci.org)
  • The novel polymer-to-protein conjugates made by reacting these reagents with. (google.com)
  • Secondary signaling component proteins Gaq, Gai, and Gao are also expressed in the rhinophore sensory epithelium.CONCLUSION:The novel rhodopsin G-protein coupled receptor-like gene subfamilies identified here do not have closely related identifiable orthologs in other metazoans, suggesting that they arose by a lineage-specific expansion as has been observed in chemosensory receptor families in other bilaterians. (ufl.edu)
  • Here we show that novel multi-transmembrane proteins with similarity to rhodopsin G-protein coupled receptors are expressed in sensory epithelia microdissected from the Aplysia rhinophore. (biomedcentral.com)
  • detection
  • The residual protein is important because of the continuing risks of transmission of prions therefore, it is necessary to use protein detection methods to check for the efficient removal of protein from surgical instruments after processing. (sychem.co.uk)
  • active-site
  • With the use of this method, we found that conversion of the Sec residue at the active site of GPx1 to DHA occurred during aging of red blood cells (RBCs) in vivo as well as in RBCs exposed to H(2)O(2) generated either externally by glucose oxidase or internally as a result of aniline-induced Hb autoxidation. (ewha.ac.kr)
  • sensory
  • In the mollusc Aplysia californica, mate-attraction is mediated by a blend of water-borne protein pheromones that are detected by sensory structures called rhinophores. (ufl.edu)
  • Secondary signaling component proteins Gα q , Gα i , and Gα o are also expressed in the rhinophore sensory epithelium. (biomedcentral.com)
  • Specifically, Chalfie reported the gene encoding Aequorea green fluorescent protein could be functionally expressed in the sensory neurons of the worm Caenorhabditis elegans (Chalfie et al. (scholarpedia.org)
  • gene
  • The biological research community, armed with the arsenal of powerful molecular biology techniques developed during the preceding two decades, was quick to recognize the unique utility of a genetically-encoded fluorophore as a marker of gene expression and protein localization. (scholarpedia.org)
  • In hindsight, the cloning of the gene and the first demonstrations of recombinant expression in non-jellyfish organisms marks a clearly discernible turning point in the history of fluorescent protein research. (scholarpedia.org)