• Structure-based design, guided by PTP mutants and x-ray protein crystallography, was used to optimize a relatively weak, nonphosphorus, nonpeptide general PTP inhibitor (2-(oxalyl-amino)-benzoic acid) into a highly selective PTP1B inhibitor. (rcsb.org)
  • Protein tyrosine phosphatase 1B (PTP1B) impairs nitric oxide (NO) production and induces endothelial dysfunction in various diseases, including diabetes, septic shock and heart failure. (crcm-marseille.fr)
  • Tunicamycin strongly increased the interactions of PTP1B with GRP78 and the activated forms of protein kinase RNA-like endoplasmic reticulum kinase (PERK) and IRE1α (proximity Ligation Assay). (crcm-marseille.fr)
  • Overexpression of Protein tyrosine phosphatase 1B (PTP1B) enzyme is responsible for type 2 diabetes and obesity. (icbb.com.np)
  • Proteins tyrosine phosphatase 1B (PTP1B) can be an established therapeutic focus on for type 2 diabetes mellitus (T2DM) and weight problems. (gasyblog.com)
  • Recombinant proteins including the catalytic domains of proteins tyrosine phosphatase 1B (PTP1B), T cell proteins tyrosine phosphatase (TCPTP), Src homology 848942-61-0 manufacture 2 (SH2) domain-containing tyrosine phosphatase 1 (SHP1), Src homology 2 (SH2) domain-containing tyrosine phosphatase 2 (SHP2), and hematopoietic proteins tyrosine phosphatase (HePTP) had been purified as previously referred to [15C18]. (gasyblog.com)
  • PTP1B is known to be regulated by oxidation, glutathionylation, phosphorylation, and SUMOlyation, and our study for the first time demonstrates the inhibition of PTP1B activity in vivo by protein molecule Grb14 in a tissue-specific manner. (edu.au)
  • Natural products possessing protein tyrosine phosphatase 1B (PTP1B) inhibitory activity found in the last decades. (stmjournals.in)
  • Together with tyrosine kinases, PTPs regulate the phosphorylation state of many important signalling molecules, such as the MAP kinase family. (wikipedia.org)
  • PTPs are increasingly viewed as integral components of signal transduction cascades, despite less study and understanding compared to tyrosine kinases. (wikipedia.org)
  • The insulin receptor (IR) and JAK1 tyrosine kinases and STAT3 can serve as direct substrates for the T-cell protein tyrosine phosphatase (TCPTP). (diabetesjournals.org)
  • Protein tyrosine phosphatases remove phosphates from the phosphotyrosine residues of target proteins and reverse the action of various protein tyrosine kinases. (mipt.ru)
  • This essential interplay between the opposing actions of protein tyrosine phosphatases and protein tyrosine kinases forms the basis of signaling networks that underlie the cellular workings of human physiology. (mipt.ru)
  • These protein kinases correspond to alternatively spliced isoforms derived from the JNK1, JNK2 and JNK3 genes. (umassmed.edu)
  • OBJECTIVE: The receptor-like protein-tyrosine phosphatase (PTP) CD148 and the nontransmembrane PTP1-B have been shown to be net positive regulators of Src family kinases in platelets. (ox.ac.uk)
  • Protein phosphatases reverse the covalent modifications of numerous cellular proteins imposed by the activation of protein kinases and, therefore, play key role in cell signaling metabolism, growth and differentiation. (bvsalud.org)
  • Protein tyrosine phosphatases (EC 3.1.3.48, systematic name protein-tyrosine-phosphate phosphohydrolase) are a group of enzymes that remove phosphate groups from phosphorylated tyrosine residues on proteins: [a protein]-tyrosine phosphate + H2O = [a protein]-tyrosine + phosphate Protein tyrosine (pTyr) phosphorylation is a common post-translational modification that can create novel recognition motifs for protein interactions and cellular localization, affect protein stability, and regulate enzyme activity. (wikipedia.org)
  • As a consequence, maintaining an appropriate level of protein tyrosine phosphorylation is essential for many cellular functions. (wikipedia.org)
  • Protein tyrosine phosphatase receptor delta (PTPRD) is a member of a large family of protein tyrosine phosphatases which negatively regulate tyrosine phosphorylation. (biomedcentral.com)
  • The JNK protein kinase is a member of the MAP kinase group that is activated in response to dual phosphorylation on threonine and tyrosine. (umassmed.edu)
  • However, they exhibited a marginal reduction in α(IIb)β(3)-mediated Src family kinase activation and tyrosine phosphorylation. (ox.ac.uk)
  • Silencing of receptor protein tyrosine phosphatase (RPTP)α, a VacA receptor, reduced VacA-induced Src phosphorylation. (elsevierpure.com)
  • Src is responsible for tyrosine phosphorylation of CagA at its Glu-Pro-Ile-Tyr-Ala (EPIYA) variant C (EPIYA-C) motif in Helicobacter pylori-infected gastric epithelial cells, resulting in binding of CagA to SHP-2 phosphatase. (elsevierpure.com)
  • Together with PROTEIN-TYROSINE KINASE, it regulates tyrosine phosphorylation and dephosphorylation in cellular signal transduction and may play a role in cell growth control and carcinogenesis. (bvsalud.org)
  • PTPs have been implicated in regulation of many cellular processes, including, but not limited to: Cell growth Cellular differentiation Mitotic cycles Oncogenic transformation Receptor endocytosis PTP activity can be found in four protein families. (wikipedia.org)
  • The class II PTPs contain only one member, low-molecular-weight phosphotyrosine phosphatase (LMPTP). (wikipedia.org)
  • Cdc25 phosphatases (dTyr and/or dThr) The Class III PTPs contains three members, CDC25 A, B, and C These are members of the HAD fold and superfamily, and include phosphatases specific to pTyr and pSer/Thr as well as small molecule phosphatases and other enzymes. (wikipedia.org)
  • Among these negative regulators, protein tyrosine phosphatases (PTPs) are likely to play a pivotal role in IR signaling. (bioone.org)
  • Several protein-tyrosine phosphatases (PTPs) have been proposed to act as negative regulators of insulin signaling. (rcsb.org)
  • Signal transduction of FMS-like tyrosine kinase 3 (FLT3) is regulated by protein-tyrosine phosphatases (PTPs). (nih.gov)
  • Despite low sequence similarity, the catalytic domain of SHP-1 shows high similarity in secondary and tertiary structures with other protein-tyrosine phosphatases (PTPs). (umassmed.edu)
  • Here, we report that high levels of the striatal-enriched protein tyrosine phosphatase (STEP), a key regulator of ERK/MAPK signaling, are found in vulnerable somatostatin-immunoreactive hilar interneurons. (jneurosci.org)
  • Our data reveal that the vulnerable cells express high levels of striatal-enriched protein tyrosine phosphatase (STEP), which is a key regulator of the extracellular signal-regulated kinase (ERK) pathway. (jneurosci.org)
  • La protéine Striatal-Enriched Protein Tyrosine Phosphatase (STEP) joue un rôle important dans la régulation de la force synaptique, notamment par sa capacité à s'opposer au renforcement synaptique et à encourager la dépression à long terme. (umontreal.ca)
  • The Striatal-Enriched Protein Tyrosine Phosphatase (STEP) plays an important role in the regulation of synaptic strength, namely through its ability to oppose synaptic strengthening and encourage long term depression. (umontreal.ca)
  • Ectopic up-regulation of PTPRD in neuroblastoma dephosphorylates tyrosine residues in AURKA resulting in a destabilization of this protein culminating in interfering with one of AURKA's primary functions in neuroblastoma, the stabilization of MYCN protein, the gene of which is amplified in approximately 25 to 30% of high risk neuroblastoma. (biomedcentral.com)
  • Sequence alignment and structural analysis suggest that the residues in the WPD loop, especially the amino acid following Asp421, are critical for the movement of WPD loop on binding substrates and the specific activity of protein-tyrosine phosphatases. (umassmed.edu)
  • An enzyme group that specifically dephosphorylates phosphotyrosyl residues in selected proteins. (bvsalud.org)
  • Protein tyrosine phosphatase nonreceptor type 2 (PTPN2) recently emerged as a promising cancer immunotherapy target. (jci.org)
  • Low tyrosine-protein phosphatase nonreceptor type 12 (PTPN12) expression may be associatedwith breast cancer growth, proliferation, and metastasis. (waocp.org)
  • Protein-tyrosine phosphatase, nonreceptor type 11 mutation analysis and clinical assessment in 45 patients with Noonan syndrome. (cdc.gov)
  • EC 3.1.3.48) catalyse the removal of a phosphate group attached to a tyrosine residue, using a cysteinyl-phosphate enzyme intermediate. (wikipedia.org)
  • Ser/Thr and Tyr dual-specificity phosphatases are a group of enzymes with both Ser/Thr (EC 3.1.3.16) and tyrosine-specific protein phosphatase (EC 3.1.3.48) activity able to remove the serine/threonine or the tyrosine-bound phosphate group from a wide range of phosphoproteins, including a number of enzymes that have been phosphorylated under the action of a kinase. (wikipedia.org)
  • Description: A sandwich ELISA kit for detection of Protein Tyrosine Phosphatase Like Protein A from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (ibiomagazine.org)
  • Description: Quantitativesandwich ELISA kit for measuring Human Receptor-type tyrosine-protein phosphatase F (PTPRF) in samples from serum, plasma, urine, tissue homogenates, cell lysates. (scalegen.com)
  • Description: A sandwich ELISA kit for detection of Protein Tyrosine Phosphatase, Non Receptor Type 11 from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (worldcarecouncil.org)
  • Endocytic trafficking dynamically regulates neuronal plasma membrane protein presentation and activity, and plays a central role in excitability and plasticity. (umassmed.edu)
  • Platelet endothelial cell adhesion molecule-1 regulates collagen-stimulated platelet function by modulating the association of phosphatidylinositol 3-kinase with Grb-2-associated binding protein-1 and linker for activation of T cells. (btvb.org)
  • CD22 is an inhibitory B cell coreceptor that regulates B cell development and activation by downregulating BCR signaling through activation of SH2-containing protein tyrosine phosphatase -1 (SHP-1). (bvsalud.org)
  • Growth factor receptor-bound protein 14 (Grb14) is an adapter protein implicated in receptor tyrosine kinase signaling. (edu.au)
  • Grb14−/− studies highlight both the positive and negative roles of Grb14 in receptor tyrosine kinase signaling in a tissue-specific manner. (edu.au)
  • The superfamily of proteins is also described vis-a-vis its complimentary protein phosphoserine/phosphoserine phosphatases. (mipt.ru)
  • PTPmu, an Ig superfamily receptor protein-tyrosine phosphatase, promotes cell-cell adhesion and interacts with the cadherin-catenin complex. (birmingham.ac.uk)
  • Description: A sandwich quantitative ELISA assay kit for detection of Human Protein Tyrosine Phosphatase, Non Receptor Type 11 (PTPN11) in samples from tissue homogenates or other biological fluids. (worldcarecouncil.org)
  • LEOPARD syndrome, also known as Noonan syndrome with multiple lentigines, is a rare autosomal dominant disorder most often caused by missense mutations in the PTPN11 gene, which encodes the protein tyrosine phosphatase SHP2. (medscape.com)
  • [ 4 ] Molecular studies have proven that LEOPARD syndrome and Noonan syndrome are allelic disorders caused by different missense mutations in PTPN11, a gene encoding the protein tyrosine phosphatase SHP-2 located at band 12q24.1. (medscape.com)
  • [ 9 ] Because the vast majority of mutations reside in and around the broad intramolecular interaction surface between the N-SH2 and PTP domains of the PTPN11 protein, they have been suggested to affect the intramolecular N-SH2/PTP binding in the absence of a phosphopeptide, leading to excessive phosphatase activities. (medscape.com)
  • [ 11 ] They revealed that whereas Noonan syndrome is caused by gain-of-function PTPN11 mutations, LEOPARD syndrome mutants are catalytically defective and act as dominant negative mutations that interfere with growth factor/Erk-mitogen-activated protein kinase-mediated signaling. (medscape.com)
  • Suppressor of cytokine signaling (SOCS)-3 and protein-tyrosine phosphatase 1B (PTP-1B) are two endogenous inhibitors of tyrosine kinase signaling pathways and suppress both insulin and leptin signaling via different molecular mechanisms. (diabetesjournals.org)
  • PRPRF is a transmembrane protein involved in regulation of insulin signaling, cell proliferation and cell migration. (antibodiesinc.com)
  • Reverse-phase protein array (RPPA) and gene set enrichment analysis (GSEA) revealed that the SRC signaling pathway is affected by PRL-3. (uky.edu)
  • The non-receptor Src homology tyrosine phosphatases Shp1/2 modulate GPVI signaling in partly opposite ways, both of which are targeted by the potential drug NSC87877. (btvb.org)
  • Therefore, PTPmu may be one of the proteins that recruits RACK1 to points of cell-cell contact, which may be important for PTPmu-dependent signaling in response to cell-cell adhesion. (birmingham.ac.uk)
  • A subclass of receptor-like protein tryosine phosphatases that contain an extracellular RDGS-adhesion recognition motif and a single cytosolic protein tyrosine phosphate domain. (online-medical-dictionary.org)
  • The term RASopathies includes disorders with mutations in the genes that code for the proteins of the RAS/MAPK pathway, such as neurofibromatosis type 1, Noonan syndrome, Legius syndrome, LEOPARD syndrome, Costello syndrome, and cardiofaciocutaneous syndrome. (medscape.com)
  • The current research emphasized on exploring the Protein-Tyrosine Phosphatase-1 B (PTP-1B) inhibitory potentials of hydroxylated chalcone derivatives against PDB ID: 4I8N (PTP-1B in complex with an inhibitor [(4-{(2s)-2-(1,3-benzoxazol-2-yl)-2-[(4-fluorophenyl)sulfamoyl]ethyl}phenyl)amino](oxo)acetic acid) by molecular docking approach using the iGEMDOCK (Genetic Evolutionary Method for Molecular Docking) software. (stmjournals.in)
  • Uncovering the Molecular Interactions in the Catalytic Loop That Modulate the Conformational Dynamics in Protein Tyrosine Phosphatase 1B. (atomistry.com)
  • A clearer understanding of this intrinsic circuitry would afford new opportunities to modulate protein function. (rcsb.org)
  • Receptor-type tyrosine-protein phosphatase F or Leukocyte Common Antigen Related protein is encoded by the gene PTPRF and is a member of the protein tyrosine phosphatase (PTP) family. (antibodiesinc.com)
  • The crystal structures of the protein-tyrosine phosphatase SHP-1 catalytic domain and the complex it forms with the substrate analogue tungstate have been determined and refined to crystallographic R values of 0.209 at 2.5 A resolution and 0.207 at 2.8 A resolution, respectively. (umassmed.edu)
  • These findings indicate that drug interference of the two phosphatases Shp1/2 subtly enhances GPVI-induced platelet responses via a mechanism not involving PLCγ2 activation, even upon PI3K inhibition. (btvb.org)
  • LMW (low-molecular-weight) phosphatases, or acid phosphatases, act on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. (wikipedia.org)
  • Although the molecular mechanisms responsible for the selective vulnerability of these cells are not well understood, activation of the extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) pathway has been implicated in neuroprotective responses to excitotoxicity in other neuronal populations. (jneurosci.org)
  • As a molecular mechanism, we demonstrate that PTPRD interacts with aurora kinase A (AURKA), an oncogenic protein that is over-expressed in multiple forms of cancer, including neuroblastoma. (biomedcentral.com)
  • Trodusquemine also prevented the Tunicamycin -induced increased arterial levels of the molecular ERS actors 78 kDa glucose-regulated protein (GRP78) and Activating Transcription Factor 6 (ATF6α). (crcm-marseille.fr)
  • The purpose of this study was to investigate the activities of the total acid phosphatase (TAP), tartrate-resistant acid phosphatase (TRAP), low molecular weight protein tyrosine phosphatase (LMW-PTP) and alkaline phosphatase (ALP) enzymes, as well as the possible correlation in the serum and in unstimulated whole saliva of children. (bvsalud.org)
  • Several inhibitors of the IR tyrosine kinase activity have recently been described and associated with human insulin resistance. (bioone.org)
  • The protein kinase activity of these JNK isoforms was measured using the transcription factors ATF2, Elk-1 and members of the Jun family as substrates. (umassmed.edu)
  • In particular, stage II and III tumors displayed enhanced PTPN2 protein expression in tumor-infiltrating T cells, and increased PTPN2 levels negatively correlated with expression of PD-1, CTLA4, STAT1, and granzyme A. In vivo, T cell- and DC-specific PTPN2 deletion reduced tumor burden in several CRC models by promoting CD44+ effector/memory T cells, as well as CD8+ T cell infiltration and cytotoxicity in the tumor. (jci.org)
  • PTPRD has a tumor suppressor function in neuroblastoma through AURKA dephosphorylation and destabilization and a downstream destabilization of MYCN protein, representing a novel mechanism for the function of PTPRD in neuroblastoma. (biomedcentral.com)
  • We further demonstrate that PTPRD has a tumor suppressor function in neuroblastoma through dephosphorylating and destabilizing AURKA, leading to a downstream decrease of MYCN protein. (biomedcentral.com)
  • Along these lines, accumulating evidence shows that the p42/44 mitogen-activated protein kinase (MAPK) pathway plays an important role in neuronal cell survival. (jneurosci.org)
  • Tyrosine phosphorylations of IR and ERK had been determined by Traditional western blotting with anti-phospho-IRand anti-phospho-ERK1/2 antibodies and had been normalized with IR and ERK proteins, respectively, that have been then determined as fold adjustments of insulin only. (gasyblog.com)
  • Membrane bound receptor forms and the cytosolic non-receptor protein tyrosine phosphatases have been described for their biological function. (mipt.ru)
  • Convincing evidence shows the role of mutations in these proteins to cause and/or intensify the severity of various diseases including metabolic and neurological disorders and also cancer. (mipt.ru)
  • Dominant role of the protein-tyrosine phosphatase CD148 in regulating platelet activation relative to protein-tyrosine phosphatase-1B. (ox.ac.uk)
  • Bulk Order Inquiry for Anti-LAR/PTPRF (Receptor-Type Tyrosine-Protein Phosphatase) Antibody FL550 Conjugate ------- (please add any order requirements, including desired quantity, timing, etc. (antibodiesinc.com)
  • At that time, glycobiology, which is the study of carbohydrates and their recognition by motif-specific carbohydrate-binding proteins or lectins, lagged far behind the studies that defined the structural and cellular biology of cell death. (nature.com)
  • The protein composition of saliva also reflects cellular signal processing that results from day-to-day environmental influences, as well as from acute or chronic stress 9 . (bvsalud.org)
  • Protein tyrosine phosphatases have slowly, yet convincingly become crucial targets for therapeutic intervention of various human pathophysiologies. (mipt.ru)
  • My results indicate that KCNK3 internalizes in response to Protein Kinase C (PKC) activation, using a novel pathway that requires the phosphoserine binding protein, 14-3-3β, and demonstrates for the first time regulated KCNK3 channel trafficking in neurons. (umassmed.edu)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Protein Tyrosine Phosphatase Like Protein A (PTPLA) in tissue homogenates, cell lysates and other biological fluids. (ibiomagazine.org)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Protein Tyrosine Phosphatase Like Protein A (PTPLA) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (ibiomagazine.org)
  • Glycans, either alone or complexed with glycan-binding proteins, can deliver intracellular signals or control extracellular processes that promote initiation, execution and resolution of cell death programs. (nature.com)
  • PRL-3 is highly expressed in patient T-ALL samples at both the mRNA and protein levels compared to normal lymphocytes. (uky.edu)
  • RESULTS Up-regulation of PTP4A2 in NPC cell lines and cells Western blotting and quantitative real-time PCR was applied to analysis the manifestation levels of PTP4A2 protein and mRNA in five NPC cell lines (CNE1, CNE2, C666, HONE1 and SUNE1) and one immortalized main nasopharyngeal epithelial cell collection (NPEC2 Bmi-1). (abt-888.net)
  • Over the course of my dissertation research I investigated endocytic mechanisms regulating two neuronal membrane proteins: the anesthetic-activated potassium leak channel, KCNK3, as well as the psychostimulant-sensitive dopamine transporter (DAT). (umassmed.edu)
  • This study addressed the role of DEP-1 for regulation of the acute myeloid leukemia (AML)-related mutant FLT3 internal tandem duplication (ITD) protein. (nih.gov)
  • Importantly, PTPN23 mutant protein was shown to have reduced phosphatase activity. (edu.sa)
  • In TGF-beta-treated MCF10A mammary epithelial cells overexpressing HER2 and by chromatin immunoprecipitation, we identified novel Smad targets including protein tyrosine phosphatase receptor type kappa (PTPRK). (nih.gov)
  • New modeling approaches reveal 'hidden' low-occupancy conformational states for protein and ligands. (rcsb.org)
  • Overall, this work demonstrates how the ensemble nature of macromolecular structure, revealed here by multitemperature crystallography, can elucidate allosteric mechanisms and open new doors for long-range control of protein function. (rcsb.org)