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Protein KinasesTyrosinePhosphorylationProtein Kinase CProtein-Tyrosine KinasesProtein Tyrosine PhosphatasesSignal TransductionCyclic AMP-Dependent Protein KinasesCalcium-Calmodulin-Dependent Protein KinasesPhosphatidylinositol 3-KinasesProtein Kinase InhibitorsEnzyme ActivationMolecular Sequence Annotationsrc-Family KinasesProtein-Serine-Threonine KinasesMolecular Sequence DataAmino Acid SequenceLymphocyte Specific Protein Tyrosine Kinase p56(lck)Enzyme InhibitorsMAP Kinase Signaling SystemCell LineCalciumCalcium-Calmodulin-Dependent Protein Kinase Type 2Mitogen-Activated Protein KinasesPhosphotyrosineCells, CulturedReceptor Protein-Tyrosine KinasesKineticsIsoenzymesProto-Oncogene ProteinsCyclic GMP-Dependent Protein KinasesCyclic AMPGenisteinCalcium-Calmodulin-Dependent Protein Kinase Type 1Protein BindingPhosphoproteinsp38 Mitogen-Activated Protein KinasesMitogen-Activated Protein Kinase 1Protein Tyrosine Phosphatase, Non-Receptor Type 1TransfectionMutationBase SequenceSubstrate SpecificityIntracellular Signaling Peptides and ProteinsPhosphatidylinositolsMitogen-Activated Protein Kinase 3ProteinsMitogen-Activated Protein Kinase KinasesBlotting, WesternProto-Oncogene Proteins c-fynProto-Oncogene Proteins pp60(c-src)CalmodulinTetradecanoylphorbol AcetateBinding SitesRecombinant Fusion ProteinsJNK Mitogen-Activated Protein KinaseseIF-2 KinaseProtein Kinase C-alphaRecombinant ProteinsProtein Structure, TertiaryProto-Oncogene Proteins c-aktVanadatesRNA, MessengerProtein Tyrosine Phosphatase, Non-Receptor Type 11src Homology DomainsSequence Homology, Amino AcidParamecium tetraureliaTumor Cells, CulturedProtein Kinase C-deltaFocal Adhesion Kinase 2Databases, GeneticTyrphostinsPhosphoprotein PhosphatasesReceptor, Epidermal Growth FactorApoptosisCell DivisionSerineMembrane ProteinsZAP-70 Protein-Tyrosine KinaseProtein Tyrosine Phosphatase, Non-Receptor Type 6Focal Adhesion Protein-Tyrosine KinasesModels, BiologicalReceptor-Like Protein Tyrosine Phosphatases, Class 2Cell MembraneType C PhospholipasesAndrostadienesPhosphotransferases (Alcohol Group Acceptor)3T3 CellsDose-Response Relationship, DrugFocal Adhesion Kinase 1Protein Tyrosine Phosphatase, Non-Receptor Type 2Cell Line, TumorCloning, MolecularGene Expression RegulationSoftwareRats, Sprague-DawleyCattlePyrimidinesIsoquinolinesImmunoblotting
SerineReceptor tyrosiInhibitorsPathwaysPhosphatasesPathwayResiduesActivate the kinaseGenesExtracellularPhosphataseERK1ReceptorsPhosphorylation sitesRegulatesEukaryoticClusterGenomeCatalyticIntracellularRTKsMutationsPI3KInteractionsSubunitRegulationMalignantPhosphorylatesRegulateSubstrateCellular proteinsAmino acidsPutativeEnsemblPleckstrinInhibitionRegulatoryDifferentiationInteractionNuclearTargetsMembranes2023BindIdentificationMotifBioinformaticsBeta
Serine7
  • Therefore, there are four main transmembrane receptor types: G protein coupled receptors (GPCRs), tyrosine kinase receptors (RTKs), serine/threonine kinase receptors (RSTKs), and ligand-gated ion channels (LGICs). (wikipedia.org)
  • This gene encodes one of the three members of the human AKT serine-threonine protein kinase family which are often referred to as protein kinase B alpha, beta, and gamma. (senescence.info)
  • These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. (senescence.info)
  • Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene. (senescence.info)
  • STP, serine/threonine protein phosphatase. (rupress.org)
  • The reversible phosphorylation of proteins on serine, threonine, and tyrosine residues represents a fundamental strategy used by eukaryotic organisms to regulate a host of biological functions, including DNA replication, cell cycle progression, energy metabolism, and cell growth and differentiation. (rupress.org)
  • Activation of MAPK is initiated by one of the four ErbB receptors (ErbB1/epidermal growth factor receptor (EgfR), ErbB2-4), which leads to signaling through Raf (RAF proto-oncogene serine/threonine-protein kinase), Mek (mitogen-activated protein kinase kinase 1/2) and Erk. (biomedcentral.com)
Receptor tyrosi2
  • Ephs belong to the largest family of receptor tyrosine kinase and are highly conserved both sequentially and structurally. (genominfo.org)
  • Receptor tyrosine kinase (RTKs), a sub-class of tyrosine kinase, regulates numerous physiological events such as cell growth, division, metabolism, and motility. (genominfo.org)
Inhibitors6
  • us pharmacological inhibitors such as Picroto in GABAA receptor antagonist, Pertussis to in Gi protein coupled receptor pathway inhibitor, Herbimycin A tyrosine kinase inhibitor, Chelerythrine chloride protein kinase C inhibitor, not Wortmannin A phosphoinositide 3 kinase inhibitor, H 89 cAMP dependent protein kinase A inhibitor for 1 hr at 37 C with 5% CO2 in humidified air prior to the addition of human SIZP. (vegfr-3inhibitor.com)
  • Results SIZP induces acrosomal e ocytosis in capacitated human sperm in a dose dependent manner A significant increase in the induction of acrosomal e o cytosis of capacitated human sperm was observed with a concomitant increase in number of zonae equivalent used per reaction, Inhibitors,Modulators,Libraries as compared to PBS. (vegfr-3inhibitor.com)
  • Trastuzumab and HER tyrosine kinase inhibitors displayed molecular synergis tic interaction with G28UCM. (cdantigens.com)
  • In contrast, the 539 hypocotyl PEGs were enriched for an auxin mediated signaling pathway, and or Inhibitors,Modulators,Libraries photo morphogenesis, including homologs of the Arabidopsis NON PHOTOTROPIC HYPOCOTYL 3 gene. (plcsignaling.com)
  • GO annotation indicated that the most enriched Inhibitors,Modulators,Libraries categories were associated with flower development and regulation, floral transition from vegetative to reproductive phase, or meristematic phase transition and transcription Inhibitors,Modulators,Libraries regulation, which is in good agreement with previous reports in soybean. (plcsignaling.com)
  • In the context of non-small cell lung cancers (NSCLC) harboring EGFR-oncogenic mutations, augmented levels of AXL and GAS6 have been found to drive resistance to EGFR tyrosine kinase inhibitors such as Erlotinib and Osimertinib in certain tumors with mesenchymal-like features. (regenerativemedicine.net)
Pathways5
  • The atypical protein kinase C (PKC) member PKC-zeta has been implicated in several signal transduction pathways regulating differentiation, proliferation or apoptosis of mammalian cells. (embl.de)
  • AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. (senescence.info)
  • Through extensive biochemical and genetic analysis, we now know that pathways are not simply switched on with kinases and off with phosphatases. (rupress.org)
  • Through these interactions, PH domains play a role in recruiting proteins to different membranes, thus targeting them to appropriate cellular compartments or enabling them to interact with other components of the signal transduction pathways. (embl.de)
  • In addition, the ErbB receptors integrate a diverse array of signals, both at the cell surface level and through cross-talk with other pathways, such as the phosphoinositide 3-kinase (Pi3k) pathway [ 11 ]. (biomedcentral.com)
Phosphatases8
  • They are involved in regulation of kinases and phosphatases, G protein associated factors and transcriptional factors. (wikipedia.org)
  • Protein phosphatases act as negative regulators of AKT proteins by dephosphorylating AKT or PIP3. (senescence.info)
  • Levels of cellular protein phosphorylation are modulated both by protein kinases and phosphatases. (rupress.org)
  • Although the importance of kinases in this process has long been recognized, an appreciation for the complex and fundamental role of phosphatases is more recent. (rupress.org)
  • Furthermore, kinases and phosphatases may work together to modulate the strength of a signal. (rupress.org)
  • Adding further complexity to this picture is the fact that both kinases and phosphatases can function in signaling networks where multiple kinases and phosphatases contribute to the outcome of a pathway. (rupress.org)
  • To fully understand this complex and essential regulatory process, the kinases and phosphatases mediating the changes in cellular phosphorylation must be identified and characterized. (rupress.org)
  • A variety of approaches, including biochemical purification, gene isolation by homology, and genetic screens, have been successfully used for the identification of putative protein kinases and phosphatases. (rupress.org)
Pathway4
  • AKT proteins also participate in the mammalian target of rapamycin (mTOR) signalling pathway which controls the assembly of the eukaryotic translation initiation factor 4F (eIF4E) complex and this pathway, in addition to responding to extracellular signals from growth factors and cytokines, is disregulated in many cancers. (senescence.info)
  • There was also an upregulation in expression of positive regulators and key components of the AMPK pathway, autophagy, proteasome function, and the unfolded protein response. (nature.com)
  • The mitogen-activated protein kinase (MAPK)/Erk pathway plays a central role in cell communication: it orchestrates signaling from external receptors to internal transcriptional machinery, which leads to changes in phenotype [ 6 , 7 ]. (biomedcentral.com)
  • Its molecular interactions were determined through KEGG analysis, and real-time PCR-based expression analysis was performed to assess its co-expression with another oncogenic cellular pathway (miR-223, KLF9, and PKCε) proteins in ovarian cancer. (biomedcentral.com)
Residues1
  • An enzyme group that specifically dephosphorylates phosphotyrosyl residues in selected proteins. (bvsalud.org)
Activate the kinase2
Genes8
  • The cellular response in signal transduction cascades involves alteration of the expression of effector genes or activation/inhibition of targeted proteins. (wikipedia.org)
  • Search results: The pahtway p3258 has 419 genes in the original annotation. (uth.edu)
  • Age-related gene expression profiles of the short-lived model animals Caenorhabditis elegans and Drosophila melanogaster share a common adult-onset expression program of genes involved in mitochondrial metabolism, DNA repair, catabolism, peptidolysis and cellular transport 3 . (nature.com)
  • The reduced expression of genes involved in mitochondrial oxidative respiration occurs in early adulthood, before the onset of functional decline, and more abruptly than a damage-response model would predict, which supports the possibility of programmed age-related transcriptional regulation 3 . (nature.com)
  • In humans, the CRYAA gene encodes a 173 amino acid residue protein by single copy genes located on chromosome 21. (molvis.org)
  • They are important post-transcriptional regulators of gene expression in plants, metazoans, and mammals, and are predicted to control the activity of 30% of all protein-coding genes [ 2 ]. (biomedcentral.com)
  • Proliferation cluster comprised 1028 genes up-regulated from days 3 to 8 of culture meanwhile the differentiation cluster was characterized by 2140 induced genes from days 15 to 21. (biomedcentral.com)
  • Proliferation was characterized by enrichment in genes involved in basic cellular and metabolic processes (transcription, ribosome biogenesis, translation and protein folding), cellular remodelling and autophagy. (biomedcentral.com)
Extracellular4
  • Poly-L-lysine (PLL) of extracellular matrix proteins is a small natural homopolymer of the essential amino acid L-lysine that is used to coat culture substrates. (hindawi.com)
  • constituting the extracellular ligand binding domain, a fibronectin domain followed by intracellular juxtamembrane kinase, and SAM domain. (genominfo.org)
  • Structurally Eph receptors are similar to RTKs, comprises extracellular region, a ligand binding and fibronectin repeat domain, whereas intracellular has a juxtamembrane, kinase, and SAM domain. (genominfo.org)
  • They can be found in telomeric proteins (6), in proteins of the extracellular matrix (7)and seemingly every macromolecular complex in between. (insulin-receptor.info)
Phosphatase1
  • 3.0: hematopoietic cell-specific Lyn substrate 1 (HCLS1), Kelch domain-containing 6 (KLHDC6), sarcoglycan delta (SGCD), KIAA1706 protein (KIAA1706), RNA guanylyltransferase and 5′-phosphatase (RNGTT), chromosome 10 open reading frame 57 (C10orf57), chromosome 9 open reading frame 52 (C9orf52), and plasminogen activator, urokinase receptor (PLAUR). (molvis.org)
ERK11
  • We also addressed the molecular inter actions of G28UCM, analysing FASN protein levels, apoptosis, and the phosphorylated forms of HER2, AKT and ERK1/2 proteins just after selleck chemical G28UCM combined with trastuzumab, erlotinib, gefitinib or lapatinib treatment method. (cdantigens.com)
Receptors1
  • All six receptors belong to the G protein-coupled receptor (GPCR) superfamily which initiates intracellular signalling cascades via four G protein classes differentiated by their α subunit type: Gαs, Gαi/o, Gαq/11, Gα12/13. (smpdb.ca)
Phosphorylation sites1
Regulates1
Eukaryotic3
  • Domain commonly found in eukaryotic signalling proteins. (embl.de)
  • Over the right time that we have worked to develop the Eukaryotic Linear Motif resource ELM, our conviction is continuing to grow that you will see more than a million LM situations in a ACY-1215 kinase inhibitor higher eukaryotic proteome. (insulin-receptor.info)
  • However, useful advances are now being made in the bioinformatics tools that address the amazing modularity of eukaryotic regulatory proteins. (insulin-receptor.info)
Cluster1
  • Regulators of small G-proteins like guanine nucleotide releasing factor GNRP (Ras-GRF) (which contains 2 PH domains), guanine nucleotide exchange proteins like vav, dbl, SoS and Saccharomyces cerevisiae CDC24, GTPase activating proteins like rasGAP and BEM2/IPL2, and the human break point cluster protein bcr. (embl.de)
Genome2
  • The benchmark genome assembly and annotation of the long-lived, cancer-resistant naked mole-rat ( Heterocephalus glaber ). (senescence.info)
  • CSL is an download Your Brain on Latino Comics: From Gus Arriola to Los Bros Hernandez of a possible DNA-binding histone cause that releases kinase of invalid genome interests in one decision, but hormone of the Canadian developments in another endocytosis. (evakoch.com)
Catalytic1
  • The juxtamembrane domain has two highly conserved tyrosine that regulate activation of catalytic core of the receptor. (genominfo.org)
Intracellular1
  • Particular C2s appear to bind phospholipids, inositol polyphosphates, and intracellular proteins. (embl.de)
RTKs1
  • Mutations reported in different functional domains of RTKs are linked to the kinase constitutive expression, ligand-independent signaling, and drug sensitivity [ 1 ]. (genominfo.org)
Mutations3
  • Mutations in Brutons tyrosine kinase (Btk) within its PH domain cause X-linked agammaglobulinaemia (XLA) in patients. (embl.de)
  • Therefore, considering the importance of Eph-ephrin class of protein in tumorigenesis, 7,620 clinically reported missense mutations belonging to the class of variables of unknown significance were retrieved from cBioPortal and evaluated for pathogenicity. (genominfo.org)
  • Considering the relevance of receptor tyrosine kinases and Eph in NSCLC, these seven mutations were assessed for change in the folding pattern using molecular dynamic simulation. (genominfo.org)
PI3K2
Interactions6
  • Protein phosphorylation can regulate enzyme function, mediate protein-protein interactions, alter subcellular localization, and control protein stability. (rupress.org)
  • Taken with each other, these benefits support the interactions between FASN and HER proteins are restricted to HER2 and do not involve the HER1 receptor. (cdantigens.com)
  • To identify proteins interacting with alpha A-crystallin (CRYAA) and to investigate the potential role that these protein interactions play in the function of CRYAA using a human proteome (HuProt) microarray. (molvis.org)
  • A HuProt microarray composed of 17,225 human full-length proteins with N-terminal glutathione S-transferase (GST) tags was used to identify protein-protein interactions. (molvis.org)
  • The protein-protein interactions may help CRYAA carry out multifaceted functions. (molvis.org)
  • To understand the functions of CRYAA and how these are regulated, protein-protein interactions need to be investigated. (molvis.org)
Subunit2
  • The binding of LPA to an LPAR allosterically activates the heterotrimeric G protein by exchanging GDP for GTP at the G protein's alpha subunit. (smpdb.ca)
  • The Gαi/o subunit inhibits the enzyme adenylyl cyclase (AC) which catalyzes the production of the important secondary messenger 3',5'-cyclic AMP (cAMP) from adenosine triphosphate (ATP). (smpdb.ca)
Regulation4
  • Regulation of protein activity mainly involves phosphorylation/dephosphorylation events, leading to its activation or inhibition. (wikipedia.org)
  • Several S. cerevisiae proteins involved in cell cycle regulation and bud formation like BEM2, BEM3, BUD4 and the BEM1-binding proteins BOI2 (BEB1) and BOI1 (BOB1). (embl.de)
  • PKCε is usually referred to as an oncogenic kinase as it is involved in the regulation of mitogenesis, cellular invasion, and survival. (biomedcentral.com)
  • INTRODUCTION Linear motifs (LMs) are short elements embedded within larger protein Rabbit polyclonal to ZFP161 sequence segments that operate as sites of regulation (1C5). (insulin-receptor.info)
Malignant1
Phosphorylates1
  • The CD4 and CD8 antigens are coupled to a protein-tyrosine kinase (p56lck) that phosphorylates the CD3 complex. (cdantigens.com)
Regulate1
  • Eph and ephrin are membrane-bound and thus regulate cell-cell interaction, migration, partitioning, and cell adhesion [ 3 ]. (genominfo.org)
Substrate2
  • Pleckstrin, the protein where this domain was first detected, is the major substrate of protein kinase C in platelets. (embl.de)
  • Binding to a protein kinase, any enzyme that catalyzes the transfer of a phosphate group, usually from ATP, to a protein substrate. (planteome.org)
Cellular proteins1
Amino acids1
  • The active full-length CRYAA protein corresponding to amino acids 1-173 of CRYAA was recombined. (molvis.org)
Putative1
  • Mouse protein citron, a putative rho/rac effector that binds to the GTP-bound forms of rho and rac. (embl.de)
Ensembl1
  • Ensembl chr 3:102,742,900. (mcw.edu)
Pleckstrin2
  • Pleckstrin homology (PH) domains are small modular domains that occur in a large variety of proteins. (embl.de)
  • Pleckstrin is one of the rare proteins to contains two PH domains. (embl.de)
Inhibition1
  • ERK inhibition on PIP protein level in MDA MB 453 and HCC 1954 cell lines. (cdantigens.com)
Regulatory1
  • These domains were first discovered as the loci of phorbol ester and diacylglycerol binding to conventional protein kinase C isozymes, which contain 2 C1 domains (C1A and C1B) in their N-terminal regulatory regions. (embl.de)
Differentiation1
  • 3) that were divided into two major clusters corresponding to the main phases observed during the preadipocyte culture: proliferation and differentiation. (biomedcentral.com)
Interaction2
  • Interaction of protein kinase C zeta with ZIP, a novel protein kinase C-binding protein. (embl.de)
  • A trans-interaction of Eph-ephrin followed by heterotetramer formation activates the signaling cascade and transforms the kinase domain from closed to open conformation which in-turn, expose the ATP binding pocket [ 5 ]. (genominfo.org)
Nuclear2
  • Cytoskeletal proteins such as dynamin (see IPR001401 ), Caenorhabditis elegans kinesin-like protein unc-104 (see IPR001752 ), spectrin beta-chain, syntrophin (2 PH domains) and S. cerevisiae nuclear migration protein NUM1. (embl.de)
  • Functional annotation clustering showed that they belong to cell cycle, organelle or nuclear lumen, protein transport, and DNA binding and repair clusters. (molvis.org)
Targets1
Membranes1
  • RNA enzymes may observe from also translocated proteins, they may phosphorylate suggested in proteins with delayed strategies, or they may outline known in membranes of factor factors. (evakoch.com)
20231
Bind3
  • Many C1 domains and C1 domain-containing proteins bind phorbol esters, but many others do not. (embl.de)
  • Hydrophobic/membrane-associated - are insoluble in water and membrane-associated, being localized at intermembrane spaces, where they can bind to membrane-associated effector proteins. (wikipedia.org)
  • The domain family possesses multiple functions including the abilities to bind inositol phosphates, and various proteins. (embl.de)
Identification1
  • This calls for extensive identification and annotation of key AR-dependent proteins and a delineation of the mechanistic action of AR. (biomedcentral.com)
Motif2
  • Ca2+-binding motif present in phospholipases, protein kinases C, and synaptotagmins (among others). (embl.de)
  • Experimentalists are similarly afflicted: while trying to identify LMs, they are likely to spend a lot of resources, time and ACY-1215 kinase inhibitor effort carrying out experiments within the false motif candidates, which usually vastly outnumber the genuine ones in any set of proteins of interest (1). (insulin-receptor.info)
Bioinformatics2
  • The identified proteins were subjected to subsequent bioinformatics analysis using the DAVID database. (molvis.org)
  • The bioinformatics problem is simple to state: LMs are too short (and the information content too poor) to be statistically significant in protein sequence searches. (insulin-receptor.info)
Beta1
  • Ser/Thr protein kinases such as the Akt/Rac family, the beta-adrenergic receptor kinases, the mu isoform of PKC and the trypanosomal NrkA family. (embl.de)