• This complex consists of three membrane proteins- alpha, beta, and gamma. (wikipedia.org)
  • The Sec61 heterotrimer forms an evolutionarily conserved channel for translocation of secreted proteins and integration of membrane proteins. (umassmed.edu)
  • Understanding integration of a-helical membrane proteins: the next steps. (umassmed.edu)
  • The trimeric Sec61/SecY complex is a protein-conducting channel (PCC) for secretory and membrane proteins. (cipsm.de)
  • The Sec61 complex forms a protein-conducting channel in the endoplasmic reticulum (ER) membrane that is required for secretion of soluble proteins and production of many membrane proteins. (biorxiv.org)
  • Membrane proteins must be threaded co-translocationally into the lipid bilayer to become membrane-integrated, often with complex topologies and typically form hetero- or homo- oligomers. (stanford.edu)
  • Growth and division model proposes that peroxisomes form by fission from pre-existing ones and peroxisomal membrane proteins (PMPs) containing vesicles from the ER fuse with them to form functional peroxisomes which can grow further and divide [12]. (5dok.net)
  • 7524) oxaloacetate decarboxylase alpha subunit CP001857 CDS Arcpr_0006 complement(7556. (go.jp)
  • 39504) translation initiation factor 2%2C alpha subunit CP001857 CDS Arcpr_0045 complement(39546. (go.jp)
  • ATP synthase F1 subunit beta [Source:H. (gsea-msigdb.org)
  • 18874) imidazoleglycerol phosphate synthase%2C cyclase subunit CP001857 CDS Arcpr_0021 19046. (go.jp)
  • 38678) Cobalamin-independent synthase MetE domain protein CP001857 CDS Arcpr_0044 complement(38683. (go.jp)
  • The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. (wikipedia.org)
  • Specifically, we are investigating the biosynthesis, translocation, processing and folding of proteins in the rough endoplasmic reticulum. (umassmed.edu)
  • A major goal of our lab is to elucidate the mechanism of protein translocation across the rough endoplasmic reticulum (RER) membrane. (umassmed.edu)
  • The in vivo kinetics of protein translocation channel gating are being analyzed using ubiquitin translocation assay (UTA) reporters. (umassmed.edu)
  • In yeast cells, protein translocation across the endoplasmic reticulum can occur by cotranslational or posttranslational pathways. (umassmed.edu)
  • Translocation of proteins through the Sec61 and SecYEG channels. (umassmed.edu)
  • Protein translocation across the rough endoplasmic reticulum. (umassmed.edu)
  • Preexisting antivector immunity can severely compromise the ability of Salmonella enterica serovar Typhimurium live vaccines to induce protective CD8 T-cell frequencies after type III secretion system-mediated heterologous protein translocation in orally immunized mice. (cipsm.de)
  • The structures indicate that Sec61 inhibitors stabilize the plug domain of Sec61 in a closed state, thereby preventing the protein-translocation pore from opening. (biorxiv.org)
  • In prokaryotes, SecYEG associates with the motor ATPase SecA to carry out translocation for pre-protein secretion. (elifesciences.org)
  • This paper explores how the protein folding process per se may be exploited to drive protein translocation. (elifesciences.org)
  • The protein must then fold during or after the translocation process. (elifesciences.org)
  • The human Sec61 complex is a widely distributed and abundant molecular machine. (amrita.edu)
  • Cryo-EM structures of the human Sec61 complex inhibited by various small-molecule inhibitors. (biorxiv.org)
  • The universally conserved heterotrimeric Sec61 complex (SecY in prokaryotes) plays essential roles in biosynthesis of more than one third of proteins in all species (for review, see ref. 1 - 4 ). (biorxiv.org)
  • The Sec61/SecY channel has an hourglass-like structure with a pore constriction (termed the pore ring) halfway across the membrane, which is gated by a movement of a plug-like ER-lumenal (or extracellular in SecY) domain of the channel 5 . (biorxiv.org)
  • In bacteria, this comprises SecY, SecE and usually SecG, with the protein-conducting pore running through the centre of SecY. (elifesciences.org)
  • showing the cavities through the channel, with the protein in grey surface, the pre-protein pore constrictions in red (SecY) or purple (SecA) mesh, and the SecY plug in red helix. (elifesciences.org)
  • The co-chaperone Hep1 is required to prevent the aggregation of mitochondrial Hsp70 proteins. (cipsm.de)
  • subunit alpha of MPP mitochondrial signal peptidase heterodimer","protein_coding" "Zm00001e017599_P001","No alias","Zea mays","Protein disulfide isomerase-like 2-2 OS=Oryza sativa subsp. (ntu.edu.sg)
  • the 18S and 28S ribosomal RNA genes and the cytochrome c oxidase subunit I (COI) gene. (bvsalud.org)
  • Biochemical experiments have revealed that the two SST3 proteins (STT3A and STT3B) assemble with a shared set of non-catalytic subunits into two separate OST complexes with different kinetic properties. (umassmed.edu)
  • The compound targets the catalytic subunit Spt14 (also referred to as Gpi3) of the fungal UDP-glycosyltransferase, the first step in GPI biosynthesis, with good selectivity over the human functional homolog PIG-A. Jawsamycin displays antifungal activity in vitro against several pathogenic fungi including Mucorales, and in vivo in a mouse model of invasive pulmonary mucormycosis due to Rhyzopus delemar infection. (nature.com)
  • They are remarkably simple with only a single catalytic subunit and carry out large domain motions during transport. (pdgf-receptor.com)
  • Transport of proteins across membranes is a fundamental process, achieved in every cell by the 'Sec' translocon. (elifesciences.org)
  • The bulk of protein secretion and membrane protein insertion is conducted by the ubiquitous Sec translocon. (elifesciences.org)
  • The SEC61A1 gene encodes for the pore-forming Sec61α subunit of the Sec61 complex. (amrita.edu)
  • This gene encodes the beta-subunit protein. (wikipedia.org)
  • Nitrate reductase delta subunit [Interproscan]. (ntu.edu.sg)
  • We determined subnanometer-resolution cryo-electron microscopy structures of eukaryotic ribosome-Sec61 complexes. (cipsm.de)
  • Protein secretion is one of the most important processes in eukaryotes, allowing diverse events from enzyme secretion in saprobes to hormonal signalling in multicellular organisms, and facilitates production of recombinant proteins in most eukaryotic production hosts. (biomedcentral.com)
  • Sigma-54 interaction domain, Bacterial regulatory protein, Propionate catabolism activator [Interproscan]. (ntu.edu.sg)
  • Protein of unknown function (DUF454) [Interproscan]. (ntu.edu.sg)
  • Binding-protein-dependent transport system inner membrane component [Interproscan]. (ntu.edu.sg)
  • FlgN protein [Interproscan]. (ntu.edu.sg)
  • Flagella basal body rod protein [Interproscan]. (ntu.edu.sg)
  • Flagella basal body rod protein, Flagellar basal body rod FlgEFG protein C-terminal [Interproscan]. (ntu.edu.sg)
  • FlgD Ig-like domain, FlgD Tudor-like domain, Flagellar hook capping protein - N-terminal region [Interproscan]. (ntu.edu.sg)
  • Flagellar L-ring protein [Interproscan]. (ntu.edu.sg)
  • Rod binding protein, Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase [Interproscan]. (ntu.edu.sg)
  • HAMP domain, Tar ligand binding domain homologue, Methyl-accepting chemotaxis protein (MCP) signalling domain [Interproscan]. (ntu.edu.sg)
  • LysR substrate binding domain, Bacterial regulatory helix-turn-helix protein [Interproscan]. (ntu.edu.sg)
  • Branched-chain amino acid transport system / permease component [Interproscan]. (ntu.edu.sg)
  • protein_coding" "EOD08104","No alias","Emiliania huxleyi","L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase [Interproscan]. (ntu.edu.sg)
  • protein_coding" "EOD09582","No alias","Emiliania huxleyi","SAM-dependent methyltransferase RsmB/NOP2-type [Interproscan]. (ntu.edu.sg)
  • protein_coding" "EOD15362","No alias","Emiliania huxleyi","ER membrane protein complex subunit 8/9 [Interproscan]. (ntu.edu.sg)
  • Protein of unknown function (DUF520) [Interproscan]. (ntu.edu.sg)
  • DMSO reductase anchor subunit (DmsC) [Interproscan]. (ntu.edu.sg)
  • Enoyl-(Acyl carrier protein) reductase [Interproscan]. (ntu.edu.sg)
  • Protein secretion is one of the most important processes in eukaryotes. (biomedcentral.com)
  • This RECON furthermore facilitated the development of three alternative methods to study protein secretion through graphical visualizations of omics data. (biomedcentral.com)
  • We have demonstrated the use of these methods to identify potential new and known targets for engineering improved growth and IgG production, as well as the general observation that CHO cells seem to have less strict transcriptional regulation of protein secretion than healthy mouse cells. (biomedcentral.com)
  • The RECON of the secretory pathway represents a strong tool for interpretation of data related to protein secretion as illustrated with transcriptomic data of Chinese Hamster Ovary (CHO) cells, the main platform for mammalian protein production. (biomedcentral.com)
  • Protein secretion is a complex process, which involves a large number of proteins and a series of steps spanning several cellular compartments. (biomedcentral.com)
  • Additionally, a number of regulatory proteins are dedicated to secure the proper response of the protein secretion pathway to environmental changes, nutrient availability, stress conditions, as well as differentiation signals [ 24 ]. (biomedcentral.com)
  • To our knowledge, so far none has built dedicated RECONs of the protein secretion pathway with a focus on the secretory components and regulators. (biomedcentral.com)
  • However, there are few well-defined biochemical reactions in protein secretion, and metabolic models fail to capture all of the regulatory processes and protein interactions. (biomedcentral.com)
  • The encapsulation and compartmentalisation of cells has necessitated the evolution of machineries that conduct proteins across membranes, including for protein secretion and organellar import. (elifesciences.org)
  • Protein transport protein Sec61 subunit beta is a protein that in humans is encoded by the SEC61B gene. (wikipedia.org)
  • On the basis of manual curation of the literature on the yeast, human, and mouse secretory pathway, we have compiled a comprehensive catalogue of characterized proteins with functional annotation and their interconnectivity. (biomedcentral.com)
  • Plastid-specific ribosomal proteins (PSRPs) have been proposed to play roles in the light-dependent regulation of chloroplast translation. (cipsm.de)
  • The advent of genome sequencing revealed in humans and other species thousands of genes encoding proteins that had not been identified by previous biochemical or genetic studies. (biorxiv.org)
  • This Review summarizes the more recent biochemical, cellular and mouse model studies that underscore the importance of the ER acetylation process in maintaining protein homeostasis and autophagy within the secretory pathway, and its impact on developmental and age-associated diseases. (biologists.com)
  • The Sec61 complex forms a transmembrane channel where proteins are translocated across and integrated into the ER membrane. (wikipedia.org)
  • The signal recognition particle (SRP), a ribonucleoprotein that binds to the polypeptide exit site on the ribosome, and the membrane bound SRP receptor (SR) function together to selectively attach a ribosome synthesizing a protein with an RER signal sequences to the Sec61 complex. (umassmed.edu)
  • Novel Sec61 mutants are designed based upon the structure of the Methanococcus janaschii SecYE b complex. (umassmed.edu)
  • Oligosaccharyltransferase (OST) catalyzes the transfer of a preassembled high-mannose oligosaccharide onto N-X-T or N-X-S acceptor sites (seqons) as the nascent polypeptide is transported into the RER lumen through the Sec61 complex. (umassmed.edu)
  • Photocross-linking of nascent chains to the STT3 subunit of the oligosaccharyltransferase complex. (umassmed.edu)
  • CCR4-NOT transcription complex subunit. (gsea-msigdb.org)
  • GINS complex subunit 2 [Source:HGNC Sy. (gsea-msigdb.org)
  • In eukaryotes, secretory proteins are first translocated into the ER by the Sec61 complex before reaching the cell surface by vesicular trafficking. (biorxiv.org)
  • The Sec61 complex also mediates membrane integration of many proteins, including most cell surface receptors and cell adhesion molecules. (biorxiv.org)
  • It is based on a highly complex machinery involving numerous proteins in several cellular compartments. (biomedcentral.com)
  • The exocyst complex, a set of eight proteins first identified from secretory mutants in yeast, is an attractive candidate for mediating directed traffic. (sdbonline.org)
  • This highly complex 'protein biogenesis' process is assisted by a diverse network of folding catalysts and protein-modifying enzymes and is scrutinized by molecular chaperones and other 'quality control' factors which ensure that only correctly folded and assembled proteins exit the ER and proceed to distal compartments of the secretory pathway. (stanford.edu)
  • According to the vesicle fusion model there are two distinct ER-derived vesicles in S. cerevisiae, one type of vesicle has the docking complex proteins (Pex13, Pex14, Pex17) and other vesicle contains the RING-finger complex proteins (Pex2, Pex10 and Pex12). (5dok.net)
  • Parts derived from human and yeast proteins are outlined with solid and dashed lines, respectively. (biorxiv.org)
  • The oligosaccharyltransferase is a hetero-octameric integral membrane protein in higher eukaryotes. (umassmed.edu)
  • 25637 PKD domain containing protein CP001857 CDS Arcpr_0030 complement(25624. (go.jp)
  • SYP7 group Qc-type SNARE protein","protein_coding" "MA_10436714g0010","No alias","Picea abies","acyl-CoA thioesterase","protein_coding" "MA_135972g0010","No alias","Picea abies","Acyl-CoA-binding domain-containing protein 3 OS=Oryza sativa subsp. (ntu.edu.sg)
  • Asparagine-linked glycosylation of proteins occurs within the lumen of the RER. (umassmed.edu)
  • The secretory pathway has two main functions: 1) performing proper folding and post-translational modifications (PTMs) of proteins e.g. glycosylation and sulfation, and 2) sorting proteins to their final cellular or extracellular destination. (biomedcentral.com)
  • Proteins synthesized at the ER are subject to covalent modifications that include N- and O-glycosylation, disulfide bond formation, and in some cases, proline and lysine hydroxylation. (stanford.edu)
  • Biosynthesis of glycosylphosphatidylinositol (GPI) is required for anchoring proteins to the plasma membrane, and is essential for the integrity of the fungal cell wall. (nature.com)
  • GPI biosynthesis is a conserved process, required for anchoring proteins to the plasma membrane of fungi and thus essential for the integrity of the fungal cell wall in yeasts and molds 4 . (nature.com)
  • Treatment with the molecular chaperone phenylbutyrate reversed the defective protein transport of renin and the imbalanced calcium homeostasis. (amrita.edu)
  • Our study reveals molecular interactions between Sec61 and its inhibitors in atomic detail and offers the structural framework for further pharmacological studies and drug design. (biorxiv.org)
  • A combination of molecular simulation with hydrogendeuterium-exchange mass spectrometry and electron paramagnetic resonance spectroscopy reveal an asymmetry across the membrane: ATP-induced conformational changes in the cytosolic cavity promote unfolded pre-protein structure, while the exterior cavity favours its formation. (elifesciences.org)
  • 9195) PhoH family protein CP001857 CDS Arcpr_0008 9278. (go.jp)
  • 41666) ExsB family protein CP001857 CDS Arcpr_0048 41748. (go.jp)
  • 45756) PEBP family protein CP001857 CDS Arcpr_0056 45795. (go.jp)
  • 47904) TATA-box binding family protein CP001857 CDS Arcpr_0059 47991. (go.jp)
  • protein_coding" "AAC74323","adhE","Escherichia coli","fused acetaldehyde-CoA dehydrogenase/iron-dependent alcohol dehydrogenase/pyruvate-formate lyase deactivase [Ensembl]. (ntu.edu.sg)
  • Here, we use a reporter gene-based screen in Saccharomyces cerevisiae for the discovery of antifungal inhibitors of GPI-anchoring of proteins, and identify the oligocyclopropyl-containing natural product jawsamycin (FR-900848) as a potent hit. (nature.com)
  • Inhibitors of PG activity, the polygalacturonase-inhibiting proteins selleck chemicals (PGIPs), have the capability to slow the hydrolytic activity of PGs and favour the accumulation of active OGs. (pdgf-receptor.com)
  • protein_coding" "AAC74156","flgA","Escherichia coli","assembly protein for flagellar basal-body periplasmic P ring [Ensembl]. (ntu.edu.sg)
  • In addition, the channel has a seam (lateral gate) in the wall that can open laterally in the plane of the membrane to release transmembrane segments (TMs) of membrane protein clients into the lipid phase. (biorxiv.org)
  • 3. Chimerical peptides according to the claim 1, characterized by containing a segment inhibitor of the activation of the NS3pro protease, this segment is a peptide able to bind to NS3pro and contact at least one residue comprised in the region Gly21-Lys28 of the N-terminal domain of the NS3pro protein from DV2 or structurally equivalent residues of the NS3pro protein from other Flavivirus. (patentsencyclopedia.com)
  • The PTS1 signal peptide is about 12 amino acids long present at the C-terminus of the protein [13]. (5dok.net)
  • chlorate resistance protein [Ensembl]. (ntu.edu.sg)
  • protein_coding" "AAC74155","flgM","Escherichia coli","anti-sigma factor for FliA (sigma 28) [Ensembl]. (ntu.edu.sg)
  • protein_coding" "AAC74157","flgB","Escherichia coli","flagellar component of cell-proximal portion of basal-body rod [Ensembl]. (ntu.edu.sg)
  • protein_coding" "AAC74158","flgC","Escherichia coli","flagellar component of cell-proximal portion of basal-body rod [Ensembl]. (ntu.edu.sg)
  • protein_coding" "AAC74163","flgH","Escherichia coli","flagellar protein of basal-body outer-membrane L ring [Ensembl]. (ntu.edu.sg)
  • inactive c-di-GMP phosphodiesterase-like EAL domain protein [Ensembl]. (ntu.edu.sg)
  • protein_coding" "AAC74185","ptsG","Escherichia coli","fused glucose-specific PTS enzymes: IIB component/IIC component [Ensembl]. (ntu.edu.sg)
  • protein_coding" "AAC74850","yeaD","Escherichia coli","D-hexose-6-phosphate epimerase-like protein [Ensembl]. (ntu.edu.sg)
  • protein_coding" "AAC74888","manY","Escherichia coli","mannose-specific enzyme IIC component of PTS [Ensembl]. (ntu.edu.sg)
  • 6097 beta-lactamase domain protein CP001857 CDS Arcpr_0005 complement(6094. (go.jp)
  • 30270 beta-lactamase domain protein CP001857 CDS Arcpr_0037 complement(30271. (go.jp)
  • The heterochromatin-enriched HP1 proteins play a critical role in regulation of transcription. (cipsm.de)
  • 40535) Transcription factor TFIIB cyclin-related protein CP001857 CDS Arcpr_0047 complement(40887. (go.jp)
  • Since the release of the first draft of the human genome sequence in 2000, the function of many of these new proteins has been identified. (biorxiv.org)
  • Using cellular disease models for ADTKD-SEC61A1, we identified an impaired protein transport of the renal secretory protein renin and a reduced abundance of regulatory calcium transporters, including SERCA2. (amrita.edu)
  • Protein conformation is critically linked to function and often controlled by interactions with regulatory factors. (cipsm.de)
  • The actual protein traffic is regulated by the organised action of numerous structural and regulatory proteins. (biomedcentral.com)
  • Previously, we proposed a Brownian ratchet model for transport, whereby the free energy of ATP-turnover favours the directional diffusion of the polypeptide (Allen et al. (elifesciences.org)
  • By employing our mouse RECON to the CHO-K1 genome in a comparative genomic approach, we could reconstruct the protein secretory pathway of CHO cells counting 764 CHO components. (biomedcentral.com)
  • The vast majority of proteins entering the secretory pathway are synthesized on ribosomes docked at ER translocons and are co-transationally translocated into the ER lumen. (stanford.edu)
  • Our goal is to elucidate the functional networks that coordinate protein synthesis and quality control in the early secretory pathway. (stanford.edu)
  • The STT3B isoform of the OST can modify skipped sequons on unfolded proteins in the lumen of the ER. (umassmed.edu)
  • Protein post-translational modifications (PTMs) take many shapes, have many effects and are necessary for cellular homeostasis. (biologists.com)
  • In order for a cell to respond to changes in internal and external environmental factors, a broad range of protein co- and post-translational modifications have evolved to expand upon the relatively static properties encoded in protein side-chains. (biologists.com)
  • sec5 mutations have been identified and characterized in Drosophila in order to delineate the role of the protein in neurons and particularly at synapses. (sdbonline.org)
  • Another distinction that has been drawn contrasts the constitutive and the regulated pathways to distinguish the ongoing transport of protein and lipid to the cell surface from the ability to secrete hormones and transmitters in response to specific stimuli. (sdbonline.org)
  • 28009) protein-L-isoaspartate O-methyltransferase CP001857 CDS Arcpr_0033 28057. (go.jp)
  • 1251 orc1%2Fcdc6 family replication initiation protein CP001857 CDS Arcpr_0002 1251. (go.jp)