• Anti-inflammatory treatment with the p38 mitogen-activated protein kinase inhibitor SB239063 is neuroprotective, decreases the number of activated microglia and facilitates neurogenesis in oxygen-glucose-deprived hippocampal slice cultures. (cbbs.eu)
  • Protein kinase C-theta critically regulates the proliferation and survival of pathogen-specific T cells in murine listeriosis. (cbbs.eu)
  • GA binding protein transcription fac. (gsea-msigdb.org)
  • This matches the MIF [GeneSymbol=MIF] transcription factor binding site V$MIF1_01 (v7.4 TRANSFAC). (gsea-msigdb.org)
  • The promoter analysis yields 174 candidate motifs, including most previously known transcription-factor binding sites and 105 new motifs. (gsea-msigdb.org)
  • In most cases, interaction between a PDZ domain and its target is constitutive, with a binding affinity of 1 to 10 microns. (embl.de)
  • However, agonist-dependent activation of cell surface receptors is sometimes required to promote interaction with a PDZ protein. (embl.de)
  • PDZ domains can occur in one or multiple copies and are nearly always found in cytoplasmic proteins. (embl.de)
  • The structure of PDZ domains allows binding to a free carboxylate group at the end of a peptide through a carboxylate-binding loop between the beta-A and beta-B strands. (embl.de)
  • There are 333040 PDZ domains in 224949 proteins in SMART's nrdb database. (embl.de)
  • Its substrate specificity suggests that Tsp may contain a substrate recognition domain, which selectively binds to the nonpolar C-termini of substrate proteins, separate from its catalytic site. (embl.de)
  • Photoaffinity labeling with a fluorescent nonpolar peptide caused the covalent attachment of the peptide to a single site on the Tsp protein. (embl.de)
  • Systematic deletion mutagenesis of Tsp localized the binding site to amino acids 206-307, a region that completely encompasses the putative PDZ domain (217-301). (embl.de)
  • Site-directed mutagenesis of a surface residue at the peptide binding site of the PDZ domain, valine 229, to Glu or Gln resulted in an increase in the K(M) value but had no effect on the k(cat) value. (embl.de)
  • In this work, we show that substrate recognition of Tsp is mediated by a PDZ domain, a small protein module that promotes protein-protein interactions by binding to internal or C-terminal sequences of their partner proteins. (embl.de)
  • In most cases, interaction between a PDZ domain and its target is constitutive, with a binding affinity of 1 to 10 microns. (embl.de)
  • PDZ domain proteins are frequently associated with the plasma membrane, a compartment where high concentrations of phosphatidylinositol 4,5-bisphosphate (PIP2) are found. (embl.de)
  • Taxonomic distribution of proteins containing PDZ domain. (embl.de)
  • The complete taxonomic breakdown of all proteins with PDZ domain is also avaliable . (embl.de)
  • Click on the protein counts, or double click on taxonomic names to display all proteins containing PDZ domain in the selected taxonomic class. (embl.de)
  • Its substrate specificity suggests that Tsp may contain a substrate recognition domain, which selectively binds to the nonpolar C-termini of substrate proteins, separate from its catalytic site. (embl.de)
  • Systematic deletion mutagenesis of Tsp localized the binding site to amino acids 206-307, a region that completely encompasses the putative PDZ domain (217-301). (embl.de)
  • The isolated PDZ domain (amino acids 206-334) is capable of folding into a well-behaved structure and binds to a nonpolar peptide with a dissociation constant (K(D)) of 1.9 microM, similar to that of the intact Tsp protein. (embl.de)
  • Site-directed mutagenesis of a surface residue at the peptide binding site of the PDZ domain, valine 229, to Glu or Gln resulted in an increase in the K(M) value but had no effect on the k(cat) value. (embl.de)
  • The use of a separate substrate recognition domain such as a PDZ domain may be a general mechanism for achieving selective protein degradation. (embl.de)