Protein that binds actin. Medical search. Frequent questions

The actin cytoskeleton is a complex structure that performs a wide range of cellular functions. (edu.au)
In particular, 1) the structure of actin was resolved from crystals in the absence of cocrystallized actin binding proteins (ABPs), 2) the prokaryotic ancestral gene of actin was crystallized and its function as a bacterial cytoskeleton was revealed, and 3) the structure of the Arp2/3 complex was described for the first time. (edu.au)
In this study, PKC betaII, but not PKC betaI, translocated to the actin cytoskeleton upon stimulation of cells with phorbol esters. (duke.edu)
The insulin receptor substrate p53 (IRSp53), an Src homology 3 (SH3) adapter protein and regulator of the actin cytoskeleton, was identified as an espin-binding protein in yeast two-hybrid screens. (northwestern.edu)
Thus, the PC espins exhibit the properties of modular actin-bundling proteins with the potential to influence the organization and dynamics of the actin cytoskeleton in PC dendritic spines and to participate in multiprotein complexes involving SH3 domain-containing proteins, such as IRSp53. (northwestern.edu)
The FLNB gene provides instructions for making a protein called filamin B. This protein helps build the network of protein filaments (cytoskeleton) that gives structure to cells and allows them to change shape and move. (medlineplus.gov)
Filamin B attaches (binds) to another protein called actin and helps the actin to form the branching network of filaments that makes up the cytoskeleton. (medlineplus.gov)
It also links actin to many other proteins to perform various functions within the cell, including the cell signaling that helps determine how the cytoskeleton will change as tissues grow and take shape during development. (medlineplus.gov)
Through this interaction, mRNAs and their associative proteins form messenger ribonucleoprotein particles (mRNPs) that are actively transported along the cytoskeleton to intracellular destinations. (frontiersin.org)
These compound mutant mice were compared to FXS mice to determine if genetically impairing pathways to the actin cytoskeleton can rescue deficits in the FXS mice. (fraxa.org)
PAK, a kinase that regulates the actin cytoskeleton downstream of Rac, also binds FMRP. (fraxa.org)
In a proof of principle, the researchers created an opto-nanobody that binds to actin, a major component of the cytoskeleton that allows cells to move, divide and respond to their environment. (scienceblog.com)
2013. The plant actin cytoskeleton responds to signals from microbe-associated molecular patterns. (purdue.edu)
involved into cell trafficking and actin cytoskeleton organization (Kim et al. (atlasgeneticsoncology.org)
A member of GTP-binding protein of the large ras superfamily involved in regulation of membrane trafficking by modulating ENDOCYTOSIS and ACTIN CYTOSKELETON remodeling. (bvsalud.org)
This thesis is focused primarily on the effect that LTD4 has on reorganisation of the actin cytoskeleton and on cell-cell and cell-matrix adhesion properties. (lu.se)
We found that LTD4 caused dramatic changes in the actin cytoskeleton in intestinal epithelial cells, and an important factor in this context was the impact of this leukotriene on the actin-binding protein vinculin, which included inducing translocation of vinculin from a cell-cell to a cell-matrix complex. (lu.se)

A central feature of actin dynamics is the capacity of filaments to polymerize and depolymerize at their ends in response to cellular conditions. (cipsm.de)
The unusual stability and direct nature of this physical interaction indicate that actin filaments represent a new class of PKC-binding protein. (rupress.org)
The PC espins efficiently bound and bundled actin filaments in vitro, and these activities were not inhibited by Ca 2+ . (northwestern.edu)
When expressed in transfected neuronal cell lines, the PC espins colocalized with actin filaments and elicited the formation of coarse cytoplasmic actin bundles. (northwestern.edu)
Cofilin can be an actin-binding protein that depolymerizes and/or TRV130 HCl (Oliceridine) severs actin filaments. (ap26113.com)
Growth-cone collapse induced by ephrin-As, a family of repulsive axon guidance molecules, is impaired upon R62D expression, resulting in perseverance of ring-shaped F-actin filaments. (jneurosci.org)
2016. Profilin-dependent nucleation and assembly of actin filaments controls cell elongation in Arabidopsis. (purdue.edu)
It comprises three major filament systems-actin, microtubules, and intermediate filaments-along with a host of adaptors, regulators, molecular motors, and additional structural proteins. (cshlpress.com)
Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). (hmdb.ca)
The extent of MDI intracellular protein binding was not affected by cytochalasin D, a chemical which binds actin filaments and inhibits active uptake into cells. (cdc.gov)
Cell-cell and cell-matrix complexes of epithelial cells are interconnected through cytoskeletal filaments and proteins, and they influence the activities and outcome of various cellular processes. (lu.se)

Identification and localization of an actin-binding motif that is unique to the epsilon isoform of protein kinase C and participates in the regulation of synaptic function. (rupress.org)
Individual isoforms of the protein kinase C (PKC) family of kinases may have assumed distinct responsibilities for the control of complex and diverse cellular functions. (rupress.org)
The binding of PKC epsilon to actin required that the kinase be activated, presumably to expose a cryptic binding site that we have identified and shown to be located between the first and second cysteine-rich regions within the regulatory domain of only this individual isoform of PKC. (rupress.org)
Once established, this protein-protein interaction securely anchored PKC epsilon to the cytoskeletal matrix while also serving as a chaperone that maintained the kinase in a catalytically active conformation. (rupress.org)
Scholars@Duke publication: Protein kinase C beta II specifically binds to and is activated by F-actin. (duke.edu)
The two most closely related isoenzymes of protein kinase C (PKC), PKC betaI and betaII, are distinct but highly homologous isoenzymes derived via alternative splicing of the same gene product. (duke.edu)
The binding of PKC betaII to actin was not inhibited by kinase inhibitors of PKC (sphingosine and staurosporine), suggesting that prior activation and/or substrate phosphorylation are not required for the interaction of PKC betaII with actin. (duke.edu)
Further, there are signaling pathways that participate in regulation, such as Wnt channel, mitogen-activated protein kinase (MAPK) channel, bone morphogenetic protein (BMPs) channel, peroxisome proliferator activated-receptors (PPARs) channel, and Notch channel. (frontiersin.org)
Image analysis of the matched maps identified 7 proteins that were either over- or down-expressed: activated protein kinase c receptor (LACK), alpha tubulin (×2), prostaglandin f2-alpha synthase, protein disulfide isomerase, vesicular transport protein and a hypothetical protein. (who.int)
Increased adhesion of the cancer cells depended on activation of cyclooxygenase-2, an enzyme that is involved in progression of colon cancers, whereas adhesion of the intestinal epithelial cells was augmented by LTD4-induced translocation of protein kinase C to areas where integrins bind to matrix proteins (focal adhesions). (lu.se)
Perilipin 1 binds to aquaporin 7 in human adipocytes and controls its mobility via protein kinase A mediated phosphorylation. (lu.se)

Moreover, R62D NLS decreases neuronal motility similar to the cytoplasmic R62D actin mutant although R62D NLS has no access to cytoplasmic actin dynamics. (jneurosci.org)
Thus, herein we provide first evidence that neuronal motility not only depends on cytoplasmic actin dynamics but also on the availability of actin to modulate nuclear functions such as gene transcription. (jneurosci.org)

Loss-of-secondary-contact mutations destabilize the Phactr1 G-actin⋅RPEL assembly. (rcsb.org)
The mutations change single protein building blocks (amino acids) in the filamin B protein or delete a small section of the protein sequence, resulting in an abnormal protein. (medlineplus.gov)
A number of single amino acid mutations linked to clinical diseases occur in the N-terminal region of cMyBP-C, including domains C0 and C1, which previously have been shown to bind to F-actin. (egelmanlab.org)
Mutations in Plasmodium falciparum actin-binding protein coronin confer reduced artemisinin susceptibility. (cdc.gov)

Expression of the actin-binding protein profilin was disrupted in the ciliate Tetrahymena thermophila by an antisense ribosome method. (bioone.org)
In cells with the antisense disruption no profilin protein was detected. (bioone.org)
Several of the mRNAs regulated by FMRP directly impact spine actin dynamics, including both the mRNA encoding the Rac GTPase, a key regulator of actin dynamics, and the mRNA encoding profilin, an actin binding protein. (fraxa.org)
2016. A processive Arabidopsis formin modulates actin-filament dynamics in association with profilin. (purdue.edu)

We show that the MT binding protein EB1 (end-binding protein 1), a key regulator of MT dynamics, can bind directly to filamentous actin (F-actin) F-actin. (umn.edu)

Many actin-binding proteins, including α-actinin, β-spectrin, dystrophin, utrophin and fimbrin, do this through the actin-binding calponin homology domain. (wikipedia.org)
The immunogen used for this product shares 77% homology with Gamma actin/actin cytoplasmic 2. (abcam.com)
Here, we designed a set of homology-directed repair donor templates that enable efficient tagging of endogenous proteins with affinity tags by transient transfection and selection of genome-edited cells in various human cell lines. (bvsalud.org)

Here we report a class of mRNA granules in human neuronal processes that are enriched in the nuclear cap-binding protein complex (CBC) and exon junction complex (EJC) core components, Y14 and eIF4AIII. (frontiersin.org)
The existence of mRNAs retaining both nuclear cap binding protein and EJC in the distal sites of neuronal processes suggests that some localized mRNAs have not yet undergone the "very first translation," which contribute to the spatio-temporal regulation of gene expression. (frontiersin.org)
Neuronal motility relies on actin treadmilling. (jneurosci.org)
We present a hitherto unappreciated cross talk of actin signaling with gene expression governing neuronal motility. (jneurosci.org)
We show that neuronal motility evoked by these actin mutants requires SRF activity. (jneurosci.org)
Conversely, actin signaling regulates neuronal SRF-mediated gene expression. (jneurosci.org)
In addition to regulation of cytoplasmic cytoskeletal dynamics, a little appreciated property of actin signaling is modulation of gene expression so far only reported for non-neuronal cells. (jneurosci.org)
Here we analyzed neuronal actin signaling by a novel approach using actin mutant isoforms known to favor or preclude actin polymerization. (jneurosci.org)
The reduced enzyme activity results in increased enzyme substrate and abnormal actin-binding proteins important in neuronal morphogenesis. (medscape.com)

The N-terminal domains of myosin binding protein C can bind polymorphically to F-actin. (egelmanlab.org)
The regulation of vertebrate striated muscle contraction involves a number of different molecules, including the thin-filament accessory proteins tropomyosin and troponin that provide Ca(2+)-dependent regulation by controlling access to myosin binding sites on actin. (egelmanlab.org)
Cardiac myosin binding protein C (cMyBP-C) appears to modulate this Ca(2+)-dependent regulation and has attracted increasing interest due to links with inherited cardiac diseases. (egelmanlab.org)
One position aligns well with the previously reported binding site that clashes with the binding of myosin to actin, but would force tropomyosin into an "on" position that exposes myosin binding sites along the filament. (egelmanlab.org)
The second position identified here would not interfere with either myosin binding or tropomyosin positioning. (egelmanlab.org)
These observations help to explain many of the seemingly contradictory results obtained with cMyBP-C and show how cMyBP-C can provide an additional layer of regulation to actin-myosin interactions. (egelmanlab.org)
Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. (hmdb.ca)

These studies serve to define a novel functional domain in the carboxyl-terminal region of PKC beta, which is involved in directing isoenzyme-specific protein-protein interactions, and consequently, isoenzyme-specific functions in vivo. (duke.edu)
Their spatial relationship is identical to the RPEL-actins within the pentavalent MRTF G-actin⋅RPEL domain complex, suggesting that conserved cooperative interactions between actin⋅RPEL units organize the assembly. (rcsb.org)
These observations suggest that EB1:F-actin interactions may negatively regulate EB1:MT interactions, and we speculate that this interaction may assist cells in differentially regulating MT stability in the actin-rich cortex as opposed to the cell interior. (umn.edu)
NMR Study of the Molecular Basis for Phosphoinositide Regulation of the Cofilin-Actin Interactions. (cathdb.info)

Actin filament dynamics govern many key physiological processes from cell motility to tissue morphogenesis. (cipsm.de)
Tuning elongation kinetics by exploiting the malleability of the filament structure may be a ubiquitous mechanism to generate a rich variety of cellular actin dynamics. (cipsm.de)
In contrast in a reducing environment even phosphatidylinositol 4 5 -bound cofilin becomes active leading to actin dynamics in the vicinity of the plasma membrane. (ap26113.com)
The dual function of cofilin namely depolymerization and severing makes it a key molecule controlling actin dynamics. (ap26113.com)
Membrane-bound dephosphorylated cofilin could be triggered by PLC-dependent PI(4 5 cleavage releasing dephosphorylated cofilin into the cytoplasm 38 40 Thereby the cytoplasmic pool of activated cofilin is increased and actin dynamics are reinforced. (ap26113.com)
In addition to regulating cytoskeletal dynamics in the cytoplasm, actin modulates nuclear gene expression. (jneurosci.org)
2015. Capping protein integrates multiple MAMP signalling pathways to modulate actin dynamics during plant innate immunity. (purdue.edu)
2014. The availability of filament ends modulates actin stochastic dynamics in live plant cells. (purdue.edu)
2014. Actin Depolymerizing Factor4 regulates actin dynamics during innate immune signaling in Arabidopsis. (purdue.edu)
We have investigated the effect of spine movement on spine calcium dynamics with two-photon photobleaching of enhanced green fluorescent protein and calcium imaging of action potential-elicited transients in spines from layer 2/3 pyramidal neurons in mouse visual cortex slices. (jneurosci.org)
We have explored the effect of spine motility on calcium dynamics by the use of two-photon excitation to photobleach enhanced green fluorescent protein (EGFP) and image calcium indicators in motile spines from layer 2/3 pyramidal neurons in slices of mouse visual cortex. (jneurosci.org)

It is demonstrated that only the natural hisactophilin DIC-HIS is capable of mediating the strong binding of monomeric actin to the monolayer, where it forms a layer of about 40 Å thickness corresponding to the average diameter of actin monomers. (hw.ac.uk)

In MCF10A/HER2 cells, TGF-beta enhanced cell motility, FAK phosphorylation, F-actin assembly, and focal adhesion formation and inhibited RhoA activity. (nih.gov)
The actin-binding protein Girdin is a hub protein that interacts with multiple proteins to regulate motility and Akt and trimeric G protein signaling in cancer cells. (fujita-hu.ac.jp)
Employing these actin mutants, we uncovered a function for actin signaling in growth-cone motility modulated by ephrin-A guidance cues. (jneurosci.org)
In this study, we demonstrate that this subset has a B. mallei -like sequence variation of the actin-based motility gene, bimA . (cdc.gov)

This may mean ability to bind actin monomers, or polymers, or both. (wikipedia.org)
In 2001, significant advances were made to our understanding of the structure and function of actin monomers. (edu.au)

In this study, using direct visualization of single actin filament elongation, we show that actin polymerization kinetics at both filament ends are strongly influenced by the binding of proteins to the lateral filament surface. (cipsm.de)
Nexilin/NEXN controls actin polymerization in smooth muscle and is regulated by myocardin family coactivators and YAP. (nih.gov)
Whether cofilin activity results in F-actin shrinking or enhanced polymerization depends on the conditions and availability of G-actin in the specific area within the cell 39 40 and is likely influenced by different signaling cascades. (ap26113.com)
CYFIP also couples Rac to WAVE-1, an activator of spine actin polymerization. (fraxa.org)
[ 2 ] The gene encodes a phosphatidylinositol (4,5) bisphosphate 5 phosphatase, localized to the trans-Golgi complex involved in actin polymerization. (medscape.com)

Many of these are likely to help us understand and distinguish between the structural models of actin microfilaments. (edu.au)

Actin-binding proteins (also known as ABPs) are proteins that bind to actin. (wikipedia.org)

In this study, we show that an isoform specific interaction between PKC epsilon and filamentous actin may serve as a necessary prelude to the enhancement of glutamate exocytosis from nerve terminals. (rupress.org)
On the other hand, the interaction of PKC betaII with actin resulted in marked enhancement of autophosphorylation of PKC betaII and in an alteration in substrate specificity. (duke.edu)
Furthermore, actin-mediated inhibition of Phactr1 nuclear import requires secondary contact residues in the Phactr1 N-terminal RPEL-N motif, suggesting that it involves interaction of RPEL-N with the C-terminal assembly. (rcsb.org)
We determined that the EB1:F-actin interaction is salt sensitive and weak under physiological salt concentrations but might be relevant in contexts where the local concentration of actin is high. (umn.edu)
The neutron reflectivity technique is applied to determine the adsorptive interaction of the 13.5-kDa actin-binding protein hisactophilin from Dictyostelium discoideum with lipid monolayers at a lateral pressure of 21 mN/m ≤ π ≤ 25 mN/m at the air-water interface. (hw.ac.uk)
The actin mutants G15S and S14C favor F-actin assembly, yet differ with regard to their ABP interaction. (jneurosci.org)
Transient Protein States in Designing Inhibitors of the MDM2-p53 Interaction. (mpg.de)
Three types of troponins exist-troponin I, troponin T, and troponin C. Each subunit has a unique function: Troponin T binds the troponin components to tropomyosin, troponin I inhibits the interaction of myosin with actin, and troponin C contains the binding sites for Ca2+ that helps initiate contraction. (medscape.com)

Both joined the Princeton faculty in 2015, and soon began working together on new ways to apply optogenetics - a set of techniques that introduce genes encoding light-responsive proteins to control cells' behaviors. (scienceblog.com)
Cancer type-specific alterations in actin genes : worth a closer look? (ugent.be)

Primarily cofilin was recognized in intranuclear actin TRV130 HCl (Oliceridine) rods pursuing treatment of the mouse fibroblast cell range C3H-ZK with dimethylsulfoxide or pursuing exposure of the cells to temperature shock 67. (ap26113.com)
Remember that 'actin/cofilin rods' usually do not bind phalloidin. (ap26113.com)
The membrane-bound small fraction of cofilin can be dephosphorylated but held inactive by binding to PI(4 5 Both cytoplasmic fraction with least a percentage from the membrane-bound cofilin are turned on by T-cell costimulation. (ap26113.com)
Moreover dephosphorylated cofilin can translocate into the nucleus 37 where it may act as actin shuttle and as chaperone for RNA polymerase II-dependent gene transcription 70-141. (ap26113.com)

Overexpressing these mutant actins in mouse hippocampal neurons not only modulated growth-cone function but also neurite elongation, which was ambiguous by traditional pharmacological interference. (jneurosci.org)
Moreover these actin mutants affect neurite elongation, an actin function which by pharmacological actin interference was ambiguous. (jneurosci.org)

2011). Filamin A is an actin crosslinking phosphoprotein of the peripheral cytoplasm and interacts with Pho proteins. (atlasgeneticsoncology.org)

In TGF-beta-treated MCF10A mammary epithelial cells overexpressing HER2 and by chromatin immunoprecipitation, we identified novel Smad targets including protein tyrosine phosphatase receptor type kappa (PTPRK). (nih.gov)
M-CSF binds to the M-CSF receptor, which is promote the proliferation and differentiation of OC and the continuous expression of RANK. (frontiersin.org)
RANK binds to transmembrane protein nuclear factor-κB receptor activating factor ligand (RANKL) to activate RANK/RANKL, TRAF6/RANKL/MAPKs, TRAF6/ RANKL/NF-κB, Wnt/RANKL/RANKL, and JAK2/STAT3/RANKL. (frontiersin.org)
The CysLT1 receptor that specifically serves as receptor for leukotriene D4 (LTD4) has been identified as a G-protein coupled receptor. (lu.se)

This gene encodes a filamentous actin-binding protein that may function in cell adhesion and migration. (nih.gov)
This gene encodes one of the connexin proteins. (nih.gov)

In the trivalent Phactr1 complex, each G-actin⋅RPEL unit makes secondary contacts with its downstream actin involving distinct RPEL residues. (rcsb.org)

In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. (hmdb.ca)

Notably, a nucleus-resident actin (R62D NLS ) also regulates SRF's transcriptional activity. (jneurosci.org)
Our previous work has shown that WAVE-1 is an important actin regulatory protein that regulates dendritic spines (a component of the synapse) and synaptic plasticity. (fraxa.org)

Many cellular processes including cell division and cell migration require coordination between the actin and microtubule (MT) cytoskeletons. (umn.edu)
Combined with technological advancements in single-particle cryogenic electron microscopy, this strategy allows efficient structural studies of endogenous proteins captured in their native cellular environment and during different cellular processes. (bvsalud.org)

Using a combination of cosedimentation, overlay, and direct binding assays, we demonstrate that filamentous actin is a principal anchoring protein for PKC epsilon within intact nerve endings. (rupress.org)

ALMS1 encodes a ~ 0.5 megadalton protein that localises to the base of centrioles. (springer.com)

Does not cross-react with adult cardiac, smooth, or skeletal muscle actin. (abcam.com)
Troponins are protein molecules that are part of cardiac and skeletal muscle. (medscape.com)

It thus appears that the ability to bind to at least two distinctly different positions on F-actin, as observed for tropomyosin, may be more common than previously considered for other actin binding proteins. (egelmanlab.org)
Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. (hmdb.ca)

Our results indicate that the active factor is either a high molecular weight protein or protein complex and is not the respiratory protein hemocyanin. (nature.com)
In line with a more complex picture, multiple isoforms of the protein likely exist and non-centrosomal sites of localisation have been reported. (springer.com)

With a $163,000 grant from the FRAXA Research Foundation, Dr. Scott Soderling and Dr. Hwan Kim bred the standard mouse model of Fragile X syndrome to their lines of mice that express reduced levels of several key proteins that modulate synaptic actin. (fraxa.org)
Our central hypothesis is that loss of FMRP leads to the deregulation of synaptic actin and that this is a significant molecular endpoint that is causal to the behavioral deficits in Fragile X Syndrome. (fraxa.org)

KMT2A gene consists of 37 exons encoding a 3969 amino-acid nuclear protein with a molecular weight of nearly 431 kDa. (atlasgeneticsoncology.org)

Intercellular adhesion molecule 5, which binds to and activates integrins, may be a direct target of perisynaptic MMP proteolysis during LTP. (nature.com)

ABSTRACT In order to define the protein expressional changes related to the process of meglumine antimoniate resistance in anthroponotic cutaneous leishmaniasis (CL), we performed a comparative proteomics analysis on sensitive and resistant strains of Leishmania tropica isolated from Iranian CL patients. (who.int)

actin nucleation 30. (ap26113.com)

SCOPe: Structural Classification of Proteins - extended. (berkeley.edu)
Differential global structural changes in the core particle of yeast and mouse proteasome induced by ligand binding. (mpg.de)

2013. Bem3, a Cdc42 GTPase-Activating Protein, traffics to an intracellular compartment and recruits the secretory Rab GTPase Sec4 to endomembranes. (purdue.edu)
Methylenediphenyl diisocyanate binds membrane and intracellular proteins of intact THP-1 cells. (cdc.gov)
Because of this low prevalence, we propose that methylenediphenyl diisocyanate (MDI) may adduct to cell membrane and intracellular proteins, contributing to the development of non-IgE asthma. (cdc.gov)
Proteins identified in the soluble fraction were all of intracellular origin suggesting that reactive MDI can cross the cell membrane and subsequently haptenate intracellular proteins. (cdc.gov)
The results of the present study support the potential involvement of dNCO haptenated membrane and intracellular proteins in development of asthma. (cdc.gov)

This abnormal protein appears to have a new, atypical function that interferes with normal proliferation or differentiation of chondrocytes, impairing ossification and leading to the signs and symptoms of atelosteogenesis type 1. (medlineplus.gov)
The second approach is to isolate actin-associated proteins using molecular and biochemical techniques, to characterize their function in vitro, and to use these as probes to dissect cytoskeletal function in living plant cells. (purdue.edu)

They were chosen because 1) they represent a family of related proteins, 2) they are widely distributed in nature, 3) an atomic structure (or at least a plausible model) is available for each of them, and 4) each is expressed in significant quantities in cells. (edu.au)
In cells, antibodies to PKC betaII, but not to PKC betaI, co-immunoprecipitated actin. (duke.edu)
We identified a group of actin-binding-bundling proteins that are expressed in cerebellar Purkinje cells (PCs) but are not detected in other neurons of the CNS. (northwestern.edu)
The antibody-like proteins, called OptoBinders, allow researchers to rapidly control processes inside and outside of cells by directing their localization, with potential applications including protein purification, the improved production of biofuels, and new types of targeted cancer therapies. (scienceblog.com)
In a pair of papers published Aug. 13 in Nature Communications, the researchers describe the creation of OptoBinders that can specifically latch onto a variety of proteins both inside and outside of cells. (scienceblog.com)
The team reported that one type of OptoBinder changed its affinity for its target molecules up to 330-fold when shifted from dark to blue light conditions, while others showed a five-fold difference in binding affinity - all of which could be useful to researchers seeking to understand and engineer the behaviors of cells. (scienceblog.com)
Crucially, OptoBinders can target proteins that are naturally present in cells, and their binding is easily reversible by changing light conditions - "a new capability that is not available to normal antibodies," said co-author José Avalos , an assistant professor of chemical and biological engineering and the Andlinger Center for Energy and the Environment . (scienceblog.com)
The ability to let go [of a target protein] is actually very valuable for many applications," said Avalos, including engineering cells' metabolisms, purifying proteins or potentially making biotherapeutics. (scienceblog.com)
We hope that this is going to be the beginning of the next era of optogenetics, opening the door to light-sensitive proteins that can interface with virtually any protein in biology, either inside or outside of cells," said Toettcher, the James A. Elkins, Jr. '41 Preceptor in Molecular Biology. (scienceblog.com)
Monobodies, on the other hand, are engineered pieces of human fibronectin, a large protein that forms part of the matrix between cells. (scienceblog.com)
After exposure, cells were processed into soluble and membrane fractions, dialyzed, and analyzed for extent of MDI protein binding, following acid hydrolysis, by quantification of the MDI hydrolysis product, methylenedianiline (MDA). (cdc.gov)
Protein bound MDI was detected in a dose-dependent manner and quantified from the membrane and soluble fractions of MDI exposed THP-1 cells. (cdc.gov)
We demonstrated this strategy by tagging six different human proteins in both HEK293T and Jurkat cells. (bvsalud.org)
In general, cell adhesion favours cell survival signalling, and integrins are the main receptors responsible for mediating the attachment of different types of cells to matrix proteins. (lu.se)

Download DNA or protein sequence, view genomic context and coordinates. (yeastgenome.org)
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. (yeastgenome.org)
Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein. (yeastgenome.org)

Each connexin protein has four transmembrane segments, two extracellular loops, a cytoplasmic loop formed between the two inner transmembrane segments, and the N- and C-terminus both being in the cytoplasm. (nih.gov)

Substrate-bound factor(s) from central nervous system-conditioned media also had a synergistic effect with laminin, suggesting a possible cooperation between humoral proteins and nervous system extracellular matrix. (nature.com)

Anatomy27

Organisms11

Chemicals and Drugs110

Analytical, Diagnostic and Therapeutic Techniques and Equipment22

Phenomena and Processes47

Disciplines and Occupations1

Technology, Industry, Agriculture3

Information Science4

Health Care1

Cytoskeleton17

Filaments11

Kinase11

Cytoplasmic actin2

Mutations4

Profilin4

Regulator1

Homology3

Neuronal9

Myosin7

Interactions4

Dynamics11

Monomeric actin1

Motility4

Monomers2

Polymerization5

Microfilaments1

ABPs1

Interaction8

Genes2

Cofilin4

Elongation2

Interacts1

Receptor4

Gene encodes2

Downstream1

Inhibits1

Regulates2

Cellular2

Filamentous1

Encodes1

Skeletal2

Tropomyosin2

Complex2

Synaptic2

Nuclear1

Molecule1

Abstract1

Nucleation1

Structural2

Intracellular5

Function2

Cells14

Sequence3

Transmembrane1

Extracellular matrix1