Protein substrate protein serine threonine kinases. Medical search. Frequent questions

Finally, by combining the sequence recognition model with the quantitative changes in phosphorylation measured in the two drug perturbation experiments, we identified 29 reliable candidates of PKA targeting residues in living cells including 8 previously known substrates. (nih.gov)
STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. (nih.gov)
The method, named Predikin, identifies key conserved substrate-determining residues in the kinase catalytic domain that contact the substrate in the region of the phosphorylation site and so determine the sequence surrounding the phosphorylation site. (biomedcentral.com)
Predikin now consists of two components: (i) PredikinDB, a database of phosphorylation sites that links substrates to kinase sequences and (ii) a Perl module, which provides methods to classify protein kinases, reliably identify substrate-determining residues, generate scoring matrices and score putative phosphorylation sites in query sequences. (biomedcentral.com)
New features in Predikin include the use of SQL queries to PredikinDB to generate predictions, scoring of predictions, more reliable identification of substrate-determining residues and putative phosphorylation sites, extended options to handle protein kinase and substrate data and an improved web interface. (biomedcentral.com)
The post-translational modification of proteins by phosphorylation of serine, threonine or tyrosine residues is a ubiquitous process in cellular regulation. (biomedcentral.com)
The second factor, termed peptide specificity, describes the interaction between amino acid residues in the catalytic domain of the protein kinase and the substrate residues that surround the phosphorylated residue. (biomedcentral.com)
Crystal structures of protein kinases with bound substrate peptides show that substrate residues at positions -3 to +3 relative to the phosphorylated serine, threonine or tyrosine residue adopt an extended conformation and bind to a pocket in the catalytic domain of the protein kinase [ 8 ]. (biomedcentral.com)
The heptapeptide sequence from -3 to +3 that best binds to the pocket is determined by the physicochemical nature of the residues in the catalytic domain that line the pocket and contact the substrate. (biomedcentral.com)
However, it is recognised that for many protein kinase families, particularly those that phosphorylate Ser/Thr residues, peptide specificity is the major factor that determines substrate specificity. (biomedcentral.com)
Protein kinases catalyse the transfer of the gamma phosphate from nucleotide triphosphates (often ATP) to one or more amino acid residues in a protein substrate side chain, resulting in a conformational change affecting protein function. (embl.de)
The reversible phosphorylation of proteins on serine, threonine, and tyrosine residues represents a fundamental strategy used by eukaryotic organisms to regulate a host of biological functions, including DNA replication, cell cycle progression, energy metabolism, and cell growth and differentiation. (rupress.org)
Experiments showed that cKMT1 could add methyl groups to lysine residues in proteins, both in controlled laboratory settings and within cells. (asbmb.org)
can phosphorylate substrate proteins on serine, threonine, tyrosines or histidine residues. (horizondiscovery.com)
Hence, substitution of serines 744 and 748 with glutamic residues as phospho-mimics bypasses the PKC requirement for PKD1 activation but does not bypass the need for antigen receptors, PLC, or DAG. (figshare.com)
DYRKs are named after their characteristic dual-specificity, as they auto-phosphorylate a conserved tyrosine in their activation loop, but phosphorylate substrates on serine and threonine residues 2 . (nature.com)
Finally, the C-terminus of HIPK1-3 comprises a region rich in serine, glutamine, and alanine (SQA) residues, which is involved in the interaction with different co-factors 14 . (nature.com)
Among these, the p21-activated kinase (PAK) group strongly favors phosphorylation of Ser over Thr residues. (cornell.edu)
PAK4, a group II PAK, almost exclusively phosphorylates its substrates on serine residues. (cornell.edu)
Eukaryotic protein kinases phosphorylate substrates at serine, threonine and tyrosine residues that fall within the context of short sequence motifs. (networkin.info)
In both human and mouse, this gene has substitutions at several residues within the ATP binding motifs that in other kinases have been shown to be required for catalysis. (utsouthwestern.edu)
Following binding of the antigen-HLA complex to the TCR, receptors aggregate, associated protein tyrosine kinases (PTK) are activated, and TCR subunits and a number of linker or adapter molecules are phosphorylated on tyrosine residues. (nih.gov)
LAT, initially characterized by this laboratory, is an integral membrane protein with multiple tyrosine residues. (nih.gov)
The specificity of serine/threonine kinases is partly determined by interactions with a few residues near the phospho-acceptor residue, forming the so-called kinase-substrate motif. (inamericawithgrace.com)
This binding interaction is mediated through the N-terminal 172 residues of Munc18c and the kinase domain residues of WNK1 (residues 159-491). (elsevierpure.com)
PTKs catalyze the transfer of the gamma-phosphoryl group from ATP to tyrosine (tyr) residues in protein substrates. (unl.edu)

To exclude potential indirect targets of PKA, we built a computational model to characterize the kinase sequence specificity toward the substrate target site based on known kinase-substrate relationships. (nih.gov)
We have previously described an approach to predicting the substrate specificity of serine-threonine protein kinases. (biomedcentral.com)
The relative contribution of substrate recruitment and peptide specificity to protein kinase substrate specificity varies between protein kinases. (biomedcentral.com)
The prediction of peptide specificity is therefore the basis for most of the available computational methods aimed at predicting substrates of protein kinases. (biomedcentral.com)
In the present review, we will deal with work performed on substrate specificity, reaction mechanism and 3D structure of E. coli MurD. (degruyter.com)
Dual specificity protein kinases (e.g. (embl.de)
Homeodomain-interacting protein kinases (HIPKs) belong to the CMGC kinase family and are closely related to dual-specificity tyrosine phosphorylation-regulated kinases (DYRKs). (nature.com)
HIPKs belong to the CMGC group of serine/threonine kinases and are part of the dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. (nature.com)
Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. (cusabio.com)
The cyclin subunit confers the substrate specificity of these complexes and differentially interacts with and activates CDK1 and CDK2 throughout the cell cycle. (wuxibiortus.com)
The specificity of this class of kinases can not be predicted. (unl.edu)
Possible dual-specificity Ser/Thr/Tyr kinase. (unl.edu)

Extracellular signal-regulated kinase (ERK) is a part of the mitogen-activated protein kinase (MAPK) signaling pathway which allows the transduction of various cellular signals to final effectors and regulation of elementary cellular processes. (mdpi.com)
It plays a role in mitogen-activated protein kinase (MAPK) signaling during cytoskeletal rearrangement, morphogenesis, and apoptosis. (umbc.edu)
Intersecting analysis, molecular docking, and pathway validation analysis showed that risperidone influences the adipocytokine signaling pathway by targeting MAPK14 (mitogen-activated protein kinase 14), MAPK8 (mitogen-activated protein kinase 8), and RXRA (retinoic acid receptor RXR-alpha), thereby inhibiting long-chain fatty acid β-oxidation by decreasing STAT3 (signal transducer and activator of transcription 3) expression and phosphorylation. (frontiersin.org)
The kinases of this family that have been suggested to play a role in apoptosis are the mitogen-activated protein kinase (MAPK) family, specifically p42/44 ERK, p38 MAPK and c-Jun N-terminal kinase (JNK), cyclic AMP-dependent protein kinase (PKA), protein kinase B (PKB), or Akt and protein kinase C (PKC). (inamericawithgrace.com)

The ribosomal protein S6 kinase 1 (S6K1) is one of two mammalian p70-RSK proteins, acting as a downstream mediator of mammalian target of rapamycin (mTOR) in the phosphoinositide 3-kinase (PI3K) pathway and/or the Ras-MAPK pathway . (axonmedchem.com)
Targeted inhibition of individual kinases of the MAPK signaling pathway using synthetic compounds represents a promising way to effective anti-cancer therapy. (mdpi.com)
Cross-talk of the MAPK signaling pathway with other proteins and signaling pathways have a crucial impact on clinical outcomes of targeted therapies and plays important role during development of drug resistance in cancers. (mdpi.com)
Mitogen-activated protein kinases such as RAF kinases involved in the MAPK signaling pathways and cyclin dependent kinases (CDKs) involved in cell cycle regulation are prime examples of pathways implicated in cancers. (horizondiscovery.com)
Orthologous to human MAPKAPK2 (MAPK activated protein kinase 2). (nih.gov)
Proinflammatory Effect of Endothelial Microparticles Is Mitochondria Mediated and Modulated Through MAPKAPK2 (MAPK-Activated Protein Kinase 2) Leading to Attenuation of Cardiac Hypertrophy. (nih.gov)
Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. (inamericawithgrace.com)

PKB contains an N-terminal pleckstrin homology (PH) domain and a C-terminal catalytic domain.The PKB-beta subfamily is part of a larger superfamily that includes the catalytic domains of other STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. (nih.gov)
Pim kinases play a major role in cell cycle regulation , anti-apoptotic activity and the homing and migration of receptor tyrosine kinases mediated via the JAK/STAT pathway . (axonmedchem.com)
Normally, upon the stimulations by growth factors (GFs), the receptor tyrosine kinases (RTKs) are activated, and then recruit PI3K protein complex on the membrane to generate phosphatidylinositol-3,4,5-triphosphate (PIP3) from phosphatidylinositol-4,5-biophosphate (PIP2) [ 2 ]. (biomedcentral.com)
The MST4 subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. (umbc.edu)
Protein tyrosine kinases of the Src family, Fyn and Lck, are associated with the TCR and TCR coreceptors, respectively. (nih.gov)
Catalytic domain of Protein Tyrosine Kinases. (unl.edu)

Serine/threonine kinases (STKs), mammalian Ste20-like protein kinase 4 (MST4) subfamily, catalytic (c) domain. (umbc.edu)
Serine/Threonine Kinases (STKs), Cyclin-Dependent protein Kinase 9 (CDK9) subfamily, catalytic (c) domain. (umbc.edu)

In peptide kinase activity assays, we find that phosphoacceptor identity impacts catalytic efficiency but does not affect the K value for both phosphorylation sites. (cornell.edu)
Knowing the phosphorylation site motif for a protein kinase facilitates designing substrates for kinase assays and mapping phosphorylation sites in protein substrates. (networkin.info)
In vitro assays indicate the protein lacks phosphorylation activity. (utsouthwestern.edu)
Interestingly, Munc18c failed to serve as a WNK1 substrate in kinase activity assays, suggesting that WNK1 functions in SNARE complex assembly outside its role as a kinase. (elsevierpure.com)

Through extensive biochemical and genetic analysis, we now know that pathways are not simply switched on with kinases and off with phosphatases. (rupress.org)
The IRS proteins (IRS1-4) are the family of adaptors regulating metabolic and mitogenic signaling pathways ( Hanke and Mann , 2009 Hanke S and Mann M (2009) The phosphotyrosine interactome of the insulin receptor family and its substrates IRS-1 and IRS-2. (scielo.br)
Activation of protein serine/threonine kinases and pathways coupled to small G proteins, and elevation of intracellular calcium, are examples of the events that follow. (nih.gov)
Akt is a serine/threonine-specific protein kinase that plays a critical role in controlling the balance between survival and death pathways in cells. (inamericawithgrace.com)
Serine/threonine (S/T) protein kinases are crucial components of diverse signaling pathways in eukaryotes, including the model filamentous fungus Neurospora crassa. (inamericawithgrace.com)

Catalytic domain of the Serine/Threonine Kinase, Cyclin-Dependent protein Kinase 9. (umbc.edu)
Functions through the formation of specific serine/threonine protein kinase holoenzyme complexes with the cyclin-dependent protein kinases CDK1 or CDK2. (wuxibiortus.com)

STP, serine/threonine protein phosphatase. (rupress.org)
The gene Protein phosphatase 1 at 87B is referred to in FlyBase by the symbol Dmel\Pp1-87B (CG5650, FBgn0004103). (yeastrc.org)
PIP3 phosphatase PTEN is inactivated upon phosphorylation in its C-terminal region by several serine/threonine kinases [ 12 ]. (biomedcentral.com)
A phosphatase is an enzyme that removes a phosphate group from a protein. (marktechpro.com)

The second messenger PIP3 recruits and activates 3-phosphoinositide dependent protein kinase-1 (PDK1) and AKT serine/threonine kinase on the membrane [ 2 ]. (biomedcentral.com)
A well-known example of a lipid kinase is phosphoinositide 3-kinase which is implicated in a number of cancers, mainly through alterations to its catalytic subunit - PIK3CA. (horizondiscovery.com)
Metz HE and Houghton AM (2011) Insulin receptor substrate regulation of phosphoinositide 3-kinase. (scielo.br)
This PTKc family is part of a larger superfamily that includes the catalytic domains of protein serine/threonine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase (PI3K). (unl.edu)

The WW domain is known to mediate regulatory protein complexes in various signaling networks, including the Hippo signaling pathway. (wikipedia.org)
Adding further complexity to this picture is the fact that both kinases and phosphatases can function in signaling networks where multiple kinases and phosphatases contribute to the outcome of a pathway. (rupress.org)
Protein phosphorylation is crucial for signaling transduction of this pathway. (biomedcentral.com)
It influences cell growth and transformation by modulating the extracellular signal-regulated kinase (ERK) pathway. (umbc.edu)

114180)-dependent protein kinases (CAMKs) are a subfamily of the serine/threonine protein kinase family. (marktechpro.com)
Tyrosine-specific kinase subfamily. (unl.edu)

Although specific for RIBOSOMAL PROTEIN S6 members of this class of kinases can act on a number of substrates within the cell. (bvsalud.org)

The p70 ribosomal S6 kinases (S6K) and p90 ribosomal S6 kinases (RSK) are distinct families of Ser/Thr kinases (EC 2.7.11.1) that regulate diverse cellular processes by phosphorylation of ribosomal protein S6 (Rps6). (axonmedchem.com)
Protein kinases and their substrates regulate essentially all cellular processes through complex regulatory networks, in which phosphorylated proteins act as switches that tune the response of the cell to environmental stimuli. (biomedcentral.com)
AGC kinase proteins are characterised by three conserved phosphorylation sites that critically regulate their function. (embl.de)
Protein phosphorylation can regulate enzyme function, mediate protein-protein interactions, alter subcellular localization, and control protein stability. (rupress.org)
p110s perform lipid kinase activity, and p85s stabilize and regulate p110s. (biomedcentral.com)
AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. (cusabio.com)

This kinase is acknowledged as a central regulator of pneumococcal cell division and cell wall synthesis, but it is still not understood when and in which order StkP phosphorylates its substrates in the course of the cell cycle. (fems-microbiology.org)

The Akt kinase family comprises three highly homologous isoforms: Akt1 (PKBα), Akt2 (PKBβ), and Akt3 (PKBγ) [4,14]. (inamericawithgrace.com)

Homeodomain-interacting protein kinases (HIPKs) are an evolutionarily conserved kinase family comprising the members HIPK1, HIPK2, and HIPK3 in vertebrates, and, additionally, HIPK4 in mammals. (nature.com)

RSK phosphorylates a variety of proteins, including transcription factors, immediate-early gene products, translational regulators, enzymes, and structural proteins, that potentially link it to many biological processes such as cell proliferation, cell differentiation, and survival. (axonmedchem.com)
Protein kinases, the enzymes responsible for protein phosphorylation, make up almost 2% of protein-encoding genes in the human genome [ 1 ] and an estimated 30-50% of human proteins are phosphorylated [ 2 ]. (biomedcentral.com)
Lysine methyltransferase, or KMT, enzymes help with the transfer of methyl groups from S-adenosyl methionine molecules to proteins. (asbmb.org)
Kinases are a large family of enzymes with over 500 encoded in the human genome and all share a highly conserved kinase domain. (horizondiscovery.com)
Together, these two families of enzymes act to modulate the activities of the proteins in a cell, typically in response to external stimuli. (marktechpro.com)
These complexes contain enzymes that are regulated by protein phosphorylation and whose substrates are located at the membrane. (nih.gov)
Regarded as constitutively active enzymes, known to participate in many, diverse biological processes, the intracellular regulation bestowed on the CK1 family of serine/threonine protein kinases is critically important, yet poorly understood. (inamericawithgrace.com)
Phosphorylation of RIBOSOMAL PROTEIN S6 by enzymes in this class results in increased expression of 5' top MRNAs. (bvsalud.org)

Pim-1, 2 and 3 (EC 2.7.11.1) make up a distinct and highly homologous family of serine/threonine kinases belonging to the Ca2+/ calmodulin -dependent protein kinase-related (CAMK) family. (axonmedchem.com)

In 2004, the first comprehensive protein-peptide interaction map for a human modular domain was reported using individually expressed WW domains and genome predicted, PPxY-containing synthetic peptides. (wikipedia.org)
Here, we describe an arrayed peptide library protocol for rapidly determining kinase phosphorylation consensus sequences. (networkin.info)
Peptide mixtures are arrayed in multiwell plates and analyzed by radiolabel assay with the kinase of interest. (networkin.info)
Engagement of the TCR by antigenic peptide bound to a histocompatibility molecule (in humans, an HLA molecule) leads to a complex set of biochemical events in which the initial activation of protein phosphorylation is critical. (nih.gov)

A family of protein serine/threonine kinases which act as intracellular signalling intermediates. (bvsalud.org)
Receptor tyr kinases (RTKs) are integral membrane proteins which contain an extracellular ligand-binding region, a transmembrane segment, and an intracellular tyr kinase domain. (unl.edu)
RTKs are usually activated through ligand binding, which causes dimerization and autophosphorylation of the intracellular tyr kinase catalytic domain, leading to intracellular signaling. (unl.edu)
Non-receptor (or cytoplasmic) tyr kinases are distributed in different intracellular compartments and are usually multi-domain proteins containing a catalytic tyr kinase domain as well as various regulatory domains such as SH3 and SH2. (unl.edu)

The AGC (cAMP-dependent, cGMP-dependent and protein kinase C) protein kinase family embraces a collection of protein kinases that display a high degree of sequence similarity within their respective kinase domains. (embl.de)
Human Similarity Proteins of target is determined by comparing the sequence similarity of all human proteins with the target based on BLAST. (idrblab.net)

My thesis work was on the understanding of the regulation of the morphogenesis and cell division by serine/threonine phosphorylation in the human pathogen Streptococcus pneumoniae . (fems-microbiology.org)

To identify kinase target substrates in a high-throughput manner, we first quantified the change of phosphoproteome in the cells of which PKA activity was perturbed by drug stimulations. (nih.gov)
Phosphorylation usually results in a functional change of the target protein by changing enzyme activity, cellular location, or association with other proteins. (embl.de)
Additionally, the researchers observed that cKMT1 modifies a crucial protein called ferredoxin-NADP(+) oxidoreductase and influences its activity, indicating its role in regulating energy transfer in Synechocystis. (asbmb.org)
Additionally, numerous novel PKCε substrates (such as BR serine/threonine kinase 1 and Abelson interactor 1) emerged that were unrelated to alcohol and anxiety but involved in critical cellular functions, such as cytoskeleton maintenance and synaptic activity. (asbmb.org)
Furthermore, these proteins may serve as biomarkers to detect abnormal PKCε activity in diseases like cancer and Alzheimer's, paving the way for new therapeutic avenues. (asbmb.org)
To tackle this, I constructed different fluorescent hybrid proteins able to report StkP activity in single pneumococcal cells, or interacting specifically with its phosphorylated substrates. (fems-microbiology.org)
Substrate-based kinase activity inference identifies MK2 as driver of colitis. (nih.gov)
Casein Kinase 2 Alpha 1 (CKII) is a serine/threonine protein kinase with structural activity. (biomedcentral.com)
The only well documented exception is LIM domain kinase 1 (LIMK1), which is phosphorylated on an activation loop threonine (Thr508) to promote its catalytic activity. (cornell.edu)
Phosphorylates FOXP3 and negatively regulates its transcriptional activity and protein stability (By similarity). (wuxibiortus.com)
Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis (PubMed:15528381, PubMed:1825810, PubMed:31548394). (inamericawithgrace.com)

Consensus peptides based on these sequences typically serve as efficient and specific kinase substrates for high throughput screening or incorporation into biosensors. (networkin.info)

1] Ribosomal Protein S6 Kinase 1 Signaling Regulates Mammalian Life Span. (axonmedchem.com)
Catalytic domain of the Protein Serine/Threonine Kinase, Mammalian Ste20-like protein kinase 4. (umbc.edu)

The serine/threonine kinase protein kinase D1 (PKD1) is a protein kinase C (PKC) substrate that mediates antigen receptor signal transduction in lymphocytes. (figshare.com)

PKC phosphorylates serines 744/748 within the PKD1 catalytic domain, and this is proposed to be necessary and sufficient for enzyme activation. (figshare.com)
Tyrosine kinase, catalytic domain. (unl.edu)
Protein Tyrosine Kinase (PTK) family, catalytic domain. (unl.edu)

Protein kinase A (PKA or cAMP-dependent protein kinase) is a serine/threonine kinase that plays essential roles in the regulation of proliferation, differentiation, and apoptosis. (nih.gov)
Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. (idrblab.net)

The WW domain (also known as the rsp5-domain or WWP repeating motif) is a modular protein domain that mediates specific interactions with protein ligands. (wikipedia.org)
Apart from the PPxY motif, certain WW domains recognize LPxY motif (where L is Leucine), and several WW domains bind to phospho-Serine-Proline (p-SP) or phospho-Threonine-Proline (p-TP) motifs in a phospho-dependent manner. (wikipedia.org)

No mass spectrometry results found for this protein. (yeastrc.org)
They used chemical-genetic methods and mass spectrometry on mouse brain lysates, identifying 39 protein substrates directly interacting with PKCε, including known and novel interactors. (asbmb.org)
Immunocoprecipitation-mass spectrometry (IP-MS), proteomic sequencing and Co-immunoprecipitation (CO-IP) were used to identify and examine the interaction between the NCAPG and CKII proteins. (biomedcentral.com)
We performed a mass spectrometry based proteomic profiling upon SOCS2 depletion and yield quantitative data for ∼4200 proteins. (elsevierpure.com)

The immunosuppressant SIROLIMUS inhibits the activation of ribosomal protein S6 kinases. (bvsalud.org)

The WW domain is one of the smallest protein modules, composed of only 40 amino acids, which mediates specific protein-protein interactions with short proline-rich or proline-containing motifs. (wikipedia.org)
No yeast two-hybrid interactions found for this protein. (yeastrc.org)
A target that has fewer human similarity proteins outside its family is commonly regarded to possess a greater capacity to avoid undesired interactions and thus increase the possibility of finding successful drugs ( Brief Bioinform, 21: 649-662, 2020 ). (idrblab.net)
Biological Network Descriptors of target is determined based on a human protein-protein interactions (PPI) network consisting of 9,309 proteins and 52,713 PPIs, which were with a high confidence score of ≥ 0.95 collected from STRING database. (idrblab.net)
The network properties of targets based on protein-protein interactions (PPIs) have been widely adopted for the assessment of target's druggability. (idrblab.net)

The RSK family of kinases: emerging roles in cellular signaling. (axonmedchem.com)
Within the HIPK family, HIPK1 and HIPK2 are the most closely related members, sharing about 93% sequence identity in their kinase domains, while HIPK3 is slightly less conserved with 87% identity. (nature.com)
This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. (utsouthwestern.edu)

Ribosomal protein S6 kinases are activated through phosphorylation in response to a variety of HORMONES and INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS. (bvsalud.org)

Furthermore, kinases and phosphatases may work together to modulate the strength of a signal. (rupress.org)
Recent studies have suggested that dopamine D2 receptor antagonists, which are used in the clinical treatment of schizophrenia, modulate protein kinase B (Akt)/glycogen synthase kinase (GSK)-3 signaling. (biomedcentral.com)

To fully understand this complex and essential regulatory process, the kinases and phosphatases mediating the changes in cellular phosphorylation must be identified and characterized. (rupress.org)
Tyrosine phosphorylation of RTKs, or adaptor proteins such as IRS-1 facilitate their binding with p85 regulatory subunits of PI3K, and then bring PI3K complex on membrane [ 2 ]. (biomedcentral.com)
Risperidone upregulates fatty acid synthase (FASN) and sterol regulatory element-binding protein 1 (SREBP1) expression in hepatocyte cultures and mouse liver by targeting the hepatic SREBP-1c/FASN couple, which is also one of the mechanisms by which risperidone induces weight gain ( 24 ). (frontiersin.org)

A variety of approaches, including biochemical purification, gene isolation by homology, and genetic screens, have been successfully used for the identification of putative protein kinases and phosphatases. (rupress.org)
It is a protein_coding_gene from Drosophila melanogaster. (yeastrc.org)
This gene is widely expressed in human tissues and its protein localizes to the nucleus. (utsouthwestern.edu)
V-Raf murine sarcoma viral oncogene homolog B1 ( B-RAF) is a gene that codes a protein B-Raf, which is a serine/ threonine-protein kinase. (inamericawithgrace.com)
STRAP (Serine/Threonine Kinase Receptor Associated Protein) is a Protein Coding gene. (inamericawithgrace.com)
Testis-specific serine kinase 3 (Tssk3) is a conserved gene, but TSSK3 kinase functions and phosphorylation substrates of TSSK3 are not known. (cdc.gov)

The research that I presented with my poster are the preliminary results regarding the development of such sensors in the bacterium S. pneumoniae , which encodes a single serine/threonine kinase called StkP. (fems-microbiology.org)

The IRS proteins function as adaptors and transmit signals from multiple receptors. (scielo.br)
In addition to binding to the insulin receptor, IRS1 also binds to and transmits signals from the receptors of prolactin, growth hormone (GH), leptin, vascular endothelial growth factor (VEGF), tropomyosin receptor kinase B (TrkB), anaplastic lymphoma kinase (ALK), insulin like growth factor (IGF1), and integrins ( Vuori and Ruoslathi , 1994 Vuori K and Ruoslahti E (1994) Association of insulin receptor substrate-1 with integrins. (scielo.br)

This domain occurred 395 times on human genes ( 910 proteins). (umbc.edu)

Gaoxiang Cao and a team at the Chinese Academy of Sciences in Wuhan recently analyzed the proteins in Synechocystis that have lysine methylation and found many proteins involved in photosynthesis that can be modified in this way. (asbmb.org)
This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag. (cusabio.com)
The serine/threonine kinase WNK1 (with no K (lysine)) has recently been implicated in exocytosis and is expressed in all three of these cell types. (elsevierpure.com)

How a particular protein kinase recognises its substrate protein(s) is therefore a key question. (biomedcentral.com)
Malectin is a membrane-anchored protein of the endoplasmic reticulum that recognises and binds Glc2-N-glycan. (unl.edu)

Many molecules are substrates for the PTKs activated by TCR engagement. (nih.gov)

This domain is found in a number of unrelated signaling and structural proteins and may be repeated up to four times in some proteins. (wikipedia.org)

cSH2 domain S690 phosphorylation decreases p85 binding affinity to tyrosine-phosphorylated proteins resulting in less PI3K membrane localization [ 15 ]. (biomedcentral.com)
These initial events lead to the generation of multiple protein complexes that localize at the TCR and the plasma membrane. (nih.gov)
Munc18c is known to be a key regulator of accessibility of the target membrane (t-SNARE) protein syntaxin 4 to participate in SNARE core complex assembly, although a paucity of Munc18c-binding factors has precluded discovery of its precise functions. (elsevierpure.com)

A large variety of proteins containing the WW domain are known. (wikipedia.org)
YAP is a WW domain-containing protein that functions as a potent oncogene. (wikipedia.org)
Because of its small size and well-defined structure, the WW domain was developed by the Gruebele and Kelly groups into a favorite subject of protein folding studies. (wikipedia.org)
The first one is located in an activation loop in the centre of the kinase domain. (embl.de)
The two other phosphorylation sites are located outside the kinase domain in a conserved region on its C-terminal side, the AGC-kinase C-terminal domain. (embl.de)
Several structures of the AGC-kinase C-terminal domain have been solved. (embl.de)
Taxonomic distribution of proteins containing S_TK_X domain. (embl.de)
The complete taxonomic breakdown of all proteins with S_TK_X domain is also avaliable . (embl.de)
Click on the protein counts, or double click on taxonomic names to display all proteins containing S_TK_X domain in the selected taxonomic class. (embl.de)
CDK9 together with a cyclin partner (cyclin T1, T2a, T2b, or K) is the main component of distinct positive transcription elongation factors (P-TEFb), which function as Ser2 C-terminal domain kinases of RNA polymerase II. (umbc.edu)
2005) Activating mutations in the tyrosine kinase domain of the epidermal growth factor receptor are associated with improved survival in gefitinib-treated chemorefractory lung adenocarcinomas. (scielo.br)
C-terminally adjacent to the HID follows a proline, glutamate, serine, and threonine (PEST)-rich domain, mediating proteasomal degradation of these kinases. (nature.com)

In vitro kinase assay is applied to identify the kinase of PIK3CA/p110α tyrosine phosphorylation. (biomedcentral.com)

Its main functions are to control substrate selectivity and enzyme stability [ 14 , 15 ]. (biomedcentral.com)
To understand the molecular and kinetic basis for PAK4 substrate selectivity we compared its mode of recognition of LIMK1 (Thr508) with that of a known serine substrate, β-catenin (Ser675). (cornell.edu)

This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. (cusabio.com)

Anatomy16

Organisms8

Diseases2

Chemicals and Drugs156

Analytical, Diagnostic and Therapeutic Techniques and Equipment20

Phenomena and Processes51

Information Science3

Health Care1

Residues26

Specificity12

Mitogen-activa4

MAPK7

Tyrosine kinases6

STKs2

Assays4

Pathways5

Cyclin-Dependent2

Phosphatase4

Phosphoinositide4

Pathway4

Subfamily2

Class of kinases1

Regulate6

Phosphorylates its substrates1

Akt21

Evolutionarily conserved1

Enzymes8

11.11

Peptide4

Intracellular4

Sequence similarity2

Morphogenesis1

Activity11

Sequences1

Mammalian2

Signal Transduction1

Catalytic domain3

Regulation2

Motif2

Mass spectrometry4

Inhibits1

Interactions5

Family3

Peptides1

Modulate2

Regulatory3

Gene6

Encodes1

Receptors2

Times on human genes1

Lysine3

Recognises2

PTKs1

Structural1

Membrane3

Domain12

PIK3CA1

Selectivity2

Downstream1