• We have observed that DAC reduced the in vitro clonogenic potential in a dose-dependent manner of both human and murine leukemia and tumor cell lines. (biomedcentral.com)
  • Click on genes, proteins and metabolites below to link to respective articles. (wikipedia.org)
  • Eukaryotic expression vectors containing genes encoding plant proteins for killing of cancer cells. (weeksmd.com)
  • consequently, genes encoding some of these proteins are being used to design constructs for the inhibition of multiplying cancer cells. (weeksmd.com)
  • Peripheral blood mononuclear cells of healthy donors and post-transplant lymphoma patients were stimulated with or without lunasin in the presence of IL-12 or IL-2. (weeksmd.com)
  • The combination of lunasin and cytokines (IL-12 plus IL-2) was capable of restoring IFNγ production by NK cells from post-transplant lymphoma patients. (weeksmd.com)
  • We measured its inhibitory activity on 17ß-HSD3 using microsomal fraction of rat testes as well as transfected LNCaP[17ß-HSD3] cells. (bvsalud.org)
  • The addition of a picolyl moiety at C-16 of RM-532-105 steroid core improves the 17ß-HSD3 inhibitory activity in the microsomal fraction of rat testes, but not in whole LNCaP[17ß-HSD3] cells. (bvsalud.org)
  • Because the inhibitory activity data against 17ß-HSD3 suggested that both steroid derivatives are non-competitive inhibitors, we performed docking and molecular dynamics simulations using a homology model of this membrane-associated enzyme. (bvsalud.org)
  • Numerous vectors have been engineered for the sole purpose of killing cancer cells, and some have successfully suppressed malignant tumours. (weeksmd.com)
  • Consistent with this hypothesis, NF-jB is constitutively acti-vated in a wide variety of tumours permitting cells to evade apoptosis [16-19,21,22]. (lsd1receptor.com)
  • This enzyme has a central role in establishing and maintaining both cytosolic and mitochondrial folylpolyglutamate concentrations and, therefore, is essential for folate homeostasis and the survival of proliferating cells. (wikipedia.org)
  • Most cancer cells undergo metabolic reprogramming that support their survival and proliferation. (degruyter.com)
  • This indicates the intricate control of metabolism and redox balance for survival of cancer cells that can be targeted by therapeutic agents. (degruyter.com)
  • The in vivo antineoplastic activity of DAC administered as an i.v. infusion was evaluated in mice with murine L1210 leukemia by measurement of survival time, and in mice bearing murine EMT6 mammary tumor by excision of tumor after chemotherapy for an in vitro clonogenic assay. (biomedcentral.com)
  • NF-jB knockdown did not induce death under normal growth conditions, but did reduce TNFa-dependent cell survival. (lsd1receptor.com)
  • Around the same time, research began to show that the regulation of iron is a central function of the immune system, and that this seems to have evolved because iron is a basic requirement for the survival and growth of cells of all types, including bacteria, parasites, and cancer. (raypeatforum.com)
  • Consequently, ROS-modulation has emerged as an anticancer strategy with synthesis of various ROS-inducing or responsive agents that target cancer cells. (degruyter.com)
  • NK activation was evaluated, and its tumoricidal activity was assessed using in vitro and in vivo tumor models. (weeksmd.com)
  • In addition, NK cells stimulated with lunasin plus cytokines displayed higher tumoricidal activity than those stimulated with cytokines alone using in vitro and in vivo tumor models. (weeksmd.com)
  • Clonogenic assays were performed on leukemic and tumor cell lines to evaluate the in vitro antineoplastic activity of DAC. (biomedcentral.com)
  • Increasing the DAC concentration and duration of exposure produced a greater loss of clonogenicity for both human leukemic and tumor cell lines. (biomedcentral.com)
  • We also observed a correlation between in vitro and in vivo concentrations needed to produce optimal responses in mice with L1210 leukemia or with EMT6 tumor. (biomedcentral.com)
  • M.S. Webb, N.L. Boman, D. Masin, D. Yapp, E. Ramsay, P.R. Cullis and M.B. Bally, "A Cationic Liposomal Vincristine Formulation with Improved Vincristine Retention, Extended Circulation Lifetime and Increased Anti-Tumor Activity", Biochim. (ubc.ca)
  • 2007). Encapsulation in liposomal nanoparticles enhances the immunostimulatory, adjuvant and anti-tumor activity of subcutaneously administered CpG ODN. (ubc.ca)
  • The Gap1 catalytic domain alone is insufficient for in vivo activity of Gap1, indicating a requirement for the additional domains. (sdbonline.org)
  • An inositol-1,3,4,5-tetrakisphosphate (IP4)-sensitive extended PH domain is essential for Gap1 activity, while Ca2+-sensitive C2 domains and a glutamine-rich region contribute equally to full activity in vivo. (sdbonline.org)
  • Resolution of these problems has been approached in Drosophila by carrying out an in vivo functional analysis of Drosophila Gap1 in the context of cone cell determination in the developing Drosophila eye. (sdbonline.org)
  • in vitro and in vivo antiviral activity of 2'-fluorinated arabinosides of 5-(2-haloalkyl)uracil. (liverpool.ac.uk)
  • 5-(2-fluoroethyl)-2'-deoxyuridine (fedu), its 2'-fluoroarabinofuranosyl analog (fefau) and the 2'-fluoroarabinofuranosyl analog (cefau) of the potent anti-herpesvirus compound 5-(2-chloroethyl)-2'-deoxyuridine (cedu) were evaluated for activity against herpes simplex virus type 1 (hsv-1) and hsv-2 in vitro and in vivo. (liverpool.ac.uk)
  • The reactivation of the TSGs ( p57KIP2 in HL-60 leukemic cells and p16CDKN2A in Calu-6 lung carcinoma cells) and the inhibition of global DNA methylation in HL-60 leukemic cells increased with DAC concentration. (biomedcentral.com)
  • an enzyme linked immunosorbent assay (elisa) system to study specific anti-herpes simplex virus (hsv) antibody production in vitro by human peripheral blood mononuclear cells (pbmc) has been developed. (liverpool.ac.uk)
  • Ras signaling can be thought of in simple terms as occuring downstream of receptor tyrosine kinases, triggered by association of adaptor proteins with the phosphorylated cytoplasmic tails of these receptors. (sdbonline.org)
  • 2005). Investivgation of factors responsible for cell line cytoplasmic expression differences. (ubc.ca)
  • Of note, an increased carbohydrate uptake and/or induction of death receptors of cancer cells was exploited to develop glycoconjugates that potentially induce cellular stress, ROS and apoptosis. (degruyter.com)
  • The exact mechanism of fen-retinide-induced cell death is not fully understood, although it is reported to induce apoptosis indepen-dently of retinoic acid receptor expression [6-9], lacking the carboxyl group required for binding to these receptors [10]. (lsd1receptor.com)
  • The in vitro antineoplastic action of DAC correlated well with its inhibition of DNA methylation and reactivation of TSG in human neoplastic cells. (biomedcentral.com)
  • Furthermore, in tumour necrosis factor alpha (TNFa)-stimulated Ewing's sarcoma (ES) cells, NF-jB is reported to prevent activation of an apoptotic cascade through inhibition of c-Jun N-terminal kinase (JNK) [23] and upregulation of anti-oxidant enzymes [24]. (lsd1receptor.com)
  • trifluorothymidine (tft) is known to be concentrated in herpes simplex virus (hsv) infected cells in vitro in the form of phosphorylated derivatives. (liverpool.ac.uk)
  • an ATP-binding ), coumarins () and terpenoids cassette (ABC) transporter (150-170 kDa), which pumps out lipo- have been reported as philic agents from cells that have entered them by free diffusion agents for overcoming multidrug resistance, and could be used aloneat very low concentrations or in combination to reverse multidrugresistance in vitro. (gotomydoctor.com)
  • In order to clarify the dose-schedule of DAC as a guide to clinical trials, we have evaluated the antineoplastic activity of DAC both in vitro and in animal models. (biomedcentral.com)
  • The underlying mechanism responsible for the effects of lunasin on NK cells is likely due to epigenetic modulation on target gene loci. (weeksmd.com)
  • Gene therapy has attracted attention for its potential to specifically and efficiently target cancer cells with minimal toxicity to normal cells. (weeksmd.com)
  • In mice with L1210 leukemia and in mice bearing EMT6 tumors, the antineoplastic action of DAC also increased with the dose. (biomedcentral.com)
  • We have shown that intensification of the DAC dose markedly increased its antineoplastic activity in mouse models of cancer. (biomedcentral.com)
  • To introduce altered Drosophila Gap1 transgenes into flies, the sevenless ( sev ) enhancer/promoter cassette, an eye-specific promoter, was used to drive expression in cone cell precursors at appropriate levels. (sdbonline.org)
  • A neoplasm characterized by abnormalities of the lymphoid cell precursors leading to excessive lymphoblasts in the marrow and other organs. (lookformedical.com)
  • One such peptide, Lunasin , has been shown to selectively act on newly transformed cells while having no cytotoxic effect on non-tumorigenic or established cancer cell lines. (weeksmd.com)
  • We then assessed its metabolic stability as well as its cytotoxic effect against a panel of cancer cell lines. (bvsalud.org)
  • Interestingly, this structural modification enhances 3-fold the metabolic stability in conjunction with a significant cytotoxic effect against pancreatic, ovarian, breast, lung, and prostate cancer cells. (bvsalud.org)
  • Cancer cells also enhance glucose-uptake to fuel these enhanced metabolic activities [ 2 ]. (degruyter.com)
  • This was independent of the increase in reactive oxygen species, p38MAPK phos-phorylation and expression of NF-jB target proteins. (lsd1receptor.com)
  • Over-expression of multidrug resistance-proteins has been shown to cause cross-resistance to doxorubicin, Cancer is one of the most important diseases in the world, killing topotecan, mitoxantrone, methotrexate and many other chemother- about every fifth or sixth person in western countries. (gotomydoctor.com)
  • it was reported that glycoprotein c of hsv-1 (gc-1) mediates the binding of c3b-coated erythrocytes to infected cells and has regulatory (decay-accelerating) activity for the alternative pathway c3 convertase of human complement. (liverpool.ac.uk)
  • This mini review highlights the development of compounds that target cancer cells by taking advantage of redox or metabolic alteration in cancer cells. (degruyter.com)
  • High abundance of glucose in the cytoplasm of a cancer cell also increases flux into other metabolic pathways such as hexosamine biosynthetic pathway (HBP) [ 7 ]. (degruyter.com)
  • In this study, we investigated the effects of Accepted 14 September 2009 nine naturally occurring compounds isolated from Citrus jambhiri Lush and Citrus pyriformis Hassk Available online 24 September 2009 (Rutaceae) for their potential to modulate the activity of P-gp in the multidrug-resistant human leukaemiacell line CEM/ADR5000. (gotomydoctor.com)
  • Treatment of drug-resistant Caco-2 cells with the most active C. jambhiri and C. pyriformis compounds increased their sensitivity to doxorubicin and completely reversed doxorubicin resistance, which agrees with a decreased P- gp activity. (gotomydoctor.com)
  • Fenretinide (N-(4-hydroxyphenyl)retinamide) is a synthetic vitamin A analogue with chemopreven-tive and therapeutic activity against a wide range of cancers [1]. (lsd1receptor.com)
  • The pioneer researcher in the role of iron in immunity believed that an excess of dietary iron contributed to the development of leukemia and lymphatic cancers. (raypeatforum.com)
  • Cancer cells often maintain high basal level of ROS and are vulnerable to any further increase in ROS levels beyond a certain protective threshold. (degruyter.com)
  • This is because cancer cells maintain high basal level of ROS, thus they are vulnerable to any further increase in ROS (Fig. 1 ). (degruyter.com)
  • Basal levels of phosphorylated NF-jB were low in all ESFT cells. (lsd1receptor.com)
  • Redirection of glutamine to cellular energetics destabilizes glutathione homeostasis in glioblastoma cells and other cancer cells [ 9 ]. (degruyter.com)
  • Bilirubin is a chemical that is produced when old red blood cells break down. (webmd.com)
  • Others may act through activation of T-CELLS or by inhibiting the activation of HELPER CELLS. (lookformedical.com)
  • this protein has previously been shown to be a delayed early herpes simplex virus type-2 specific protein. (liverpool.ac.uk)
  • infection of human epidermoid carcinoma no. 2 cells with herpes simplex virus type 1 (hsv-1) leads to a reorganization of antigens associated with both the small and heterogeneous nuclear ribonucleoprotein complexes (snrnp and hnrnp). (liverpool.ac.uk)
  • BAY 11-7082-induced cell death through an NF-jB-independent mechanism and enhanced cell death when combined with fenretinide. (lsd1receptor.com)
  • using [4-14c]edu as the radiolabeled analogue of edu, it was ascertained that, at antivirally active doses, edu is phosphorylated to a much greater extent by hsv-infected vero cells than by mock-infected cells. (liverpool.ac.uk)
  • In most cell types, NF-jB is sequestered in the cytoplasm by association with IjBa [20]. (lsd1receptor.com)
  • Adding lunasin to IL-12- or IL-2-stimulated NK cells demonstrated synergistic effects in the induction of IFNG and GZMB involved in cytotoxicity. (weeksmd.com)
  • 2008) The effect of circulation lifetime and drug-to-lipid ratio of intravenously administered lipid nanoparticles on the biodistribution and immunostimulatory activity of encapsulated CpG-ODN. (ubc.ca)
  • The drug is used in the therapy of acute leukemia. (lookformedical.com)
  • of cell death [7]. (lsd1receptor.com)
  • In this study we have investigated the role of NF-jB in fenretinide-induced cell death and the importance of NF-jB in ESFT. (lsd1receptor.com)
  • upon elevation of Ca2+ and IP4 concentrations, the PH domain binds to IP4 thereby stimulating catalytic activity, while at the same time the C2a domain takes over the role of membrane tether. (sdbonline.org)
  • the hnrnp core protein antigens remain associated with the host chromatin, which appears to collapse into internal aggregates and along the nuclear envelope. (liverpool.ac.uk)
  • They frequently play an anti-apopto-tic role [14-16], but may also be pro-apoptotic in some cell types and conditions [16-19]. (lsd1receptor.com)
  • It interferes with nucleic acid synthesis by inhibiting purine metabolism and is used, usually in combination with other drugs, in the treatment of or in remission maintenance programs for leukemia. (lookformedical.com)
  • It has been used similarly to MERCAPTOPURINE in the treatment of leukemia. (lookformedical.com)
  • the system is designed to identify and type hsv from direct lesion smears (ds) and from cell culture smears (cs) infected with a swab from the patient's lesion by means of an indirect fluorescence assay (ifa) using hsv type-common and hsv-2 type-specific monoclonal antibodies. (liverpool.ac.uk)