Protein rna polymerase iii. Medical search. Frequent questions

This gene provides instructions for making the largest piece (subunit) of an enzyme called RNA polymerase III. (medlineplus.gov)
The POLR3A gene mutations that cause Wiedemann-Rautenstrauch syndrome lead to production of abnormal subunit proteins. (medlineplus.gov)
Co-transcriptional pre-mRNA processing relies on reversible phosphorylation of the carboxyl-terminal domain (CTD) of Rpb1, the largest subunit of RNA polymerase II (RNAP II). (cipsm.de)
The protein encoded by this gene is a subunit of both RNA polymerase I and RNA polymerase III complexes. (nih.gov)
We report that HTT forms a transcription-coupled DNA repair (TCR) complex with RNA polymerase II subunit A (POLR2A), ataxin-3, the DNA repair enzyme polynucleotide-kinase-3'-phosphatase (PNKP), and cyclic AMP-response element-binding (CREB) protein (CBP). (elifesciences.org)
Delta subunit is a two domain protein. (xray.cz)
The hRPC62 subunit of human RNA polymerase III displays helicase activity. (mpg.de)

TFIIIB consists of three subunits: TATA binding protein (TBP), a TFIIB-related factor (BRF1, or BRF2 for transcription of a subset of Pol III-transcribed genes in vertebrates), and a B-double-prime (BDP1) unit. (wikipedia.org)
subunits: 1, 2, 3, 4, 5) (also termed PBP and PTF) binds to the PSE (Proximal Sequence Element) centered approximately 55 base pairs upstream of the start site of transcription. (wikipedia.org)
The abnormal subunits may prevent assembly of the RNA polymerase III enzyme or result in an RNA polymerase III with impaired ability to synthesize RNA. (medlineplus.gov)
Pol II and Pol III are protein complexes consisting of 12 and 17 subunits. (cipsm.de)
HA is a trimer (which is comprised of three subunits), while NA is a tetramer (which is comprised of four subunits and its head region resembles a 4-leaf clover). (cdc.gov)
Each of the 8 RNA molecules is associated with many copies of a nucleoprotein, several molecules of the three subunits of its RNA polymerase some "non-structural" protein molecules of uncertain function. (prospecbio.com)
The RNA polymerase of Gram positive bacteria consists of seven subunits. (xray.cz)
Human Pol III is composed of 17 subunits. (mpg.de)
Three specific Pol III subunits form a stable ternary subcomplex (RPC62-RPC39-RPC32α/β) being involved in pre-initiation complex formation. (mpg.de)

The process of transcription (by any polymerase) involves three main stages: Initiation, requiring construction of the RNA polymerase complex on the gene's promoter Elongation, the synthesis of the RNA transcript Termination, the finishing of RNA transcription and disassembly of the RNA polymerase complex Initiation: the construction of the polymerase complex on the promoter. (wikipedia.org)
This enzyme is involved in the production (synthesis) of ribonucleic acid (RNA), a chemical cousin of DNA. (medlineplus.gov)
Initiation of BAG-1 protein synthesis can occur by both cap-dependent and cap-independent mechanisms and it has been shown that synthesis of BAG-1S is dependent upon the presence of an internal ribosome entry segment (IRES) in the 5′-UTR of BAG-1 mRNA. (nature.com)
Here, we show that the BAG-1 IRES maintains synthesis of BAG-1 protein following exposure of cells to the chemotoxic drug vincristine but not to cisplatin and that this is brought about, in part, by the relocalization of PTB and PCBP1 from the nucleus to the cytoplasm. (nature.com)
The initial objective of this study was to determine whether a co-regulation of the three Pols for synthesis of ribosomal components could be demonstrated in human cells. (europa.eu)
In vitro synthesis of RNA requires a purified DNA plasmid, purified RNA polymerase, metal-ion enzyme cofactors, and raw-material nucleotides. (bioprocessintl.com)
The plus strand genomic RNA is first used to synthesize the minus strand genomic RNA complement, which is subsequently used as a template for synthesis of plus strand viral RNA genomes that are packaged into mature virions. (reactome.org)
Then mRNA synthesis occurs (in the 5' to 3' direction). (kenyon.edu)
RNA polymerase plays a fundamental role in the process of protein synthesis because it is responsible for the DNA transcription. (xray.cz)
Reverse transcription is the synthesis of a complementary DNA sequence from an RNA template using reverse transcriptase, which is an RNA-dependent DNA polymerase. (medscape.com)

Our study provides high-resolution structures of medically relevant FluPol A , as well as insights into the replication mechanisms of the viral RNA genome. (nature.com)
Findings from the new study-published recently in Nature through an article titled, " A high-resolution protein architecture of the budding yeast genome "-mapped precise binding sites of more than 400 different chromosomal proteins in the yeast genome, most of which regulate the expression of genes. (genengnews.com)
In the current study, the team used a technique called ChIP-exo to map the binding locations of about 400 different proteins that interact with the yeast genome, some at a few locations and others at thousands of locations. (genengnews.com)
Analysis of so much data required the use of Penn State's supercomputing clusters and the development of several novel bioinformatic tools to identify patterns and reveal the organization of regulatory proteins in the yeast genome. (genengnews.com)
The analysis revealed a surprisingly small number of unique protein assemblages that are used repeatedly across the yeast genome. (genengnews.com)
The plus strand RNA genome of the human SARS coronavirus 1 (SARS-CoV-1) is replicated by the viral replication-transcription complex (RTC) composed of nonstructural proteins nsp3-nsp16, encoded by open reading frames ORF1a and ORF1b. (reactome.org)
It should also be noted that the core genome is arranged in a collinear manner and is the largest part of the genome, even though it does not confer as many different proteins and enzymes. (kenyon.edu)
These individual regions, scattered throughout the genome, differ in metabolism and confer many different proteins and enzymes that contribute to the diversity in the bacteria. (kenyon.edu)
In participating UK research institutions, investigators can publish open access in Genome Research, Genes & Development, RNA, and Learning & Memory without article publication charges and all staff can read the entire renowned Cold Spring Harbor journal collection. (cshlpress.com)

These factors and promoter elements are shared between Pol II and Pol III transcription of snRNA genes. (wikipedia.org)
Predicted to be involved in 5S class rRNA transcription by RNA polymerase III and transcription initiation from RNA polymerase III promoter. (nih.gov)
Fig. 3: Structures of H3N2 FluPol A bound to cRNA promoter. (nature.com)
Transcription is initiated by the binding of RNAP (RNA polymerase) to the promoter. (kenyon.edu)
The bacterial activator protein NorR binds to enhancer-like elements, upstream of the promoter site, and activates 54-dependent transcription of genes that encode nitric oxide detoxifying enzymes (NorVW), in response to NO stress. (strath.ac.uk)
Unique to the norVW promoter in Escherichia coli is the presence of three enhancer sites associated with a binding site for 54-RNA polymerase. (strath.ac.uk)
We demonstrate that this is essentially due to the need for all three enhancers for maximal ATPase activity of NorR, energy from which is used to remodel the closed E54 complex and allow melting of the promoter DNA. (strath.ac.uk)

Zinc inhibits SARS-CoV RNA polymerase, and thus its replication capacity. (frontiersin.org)
serial twofold dilutions of each primer against a constant amount of purified SARS-CoV RNA. (cdc.gov)

Diagnosis was based on reverse transcription polymerase chain reaction, and patients were classified as mild, moderate or critical. (who.int)
Among 20 laboratories testing Module A, 17 (85%) correctly detected DENV RNA by reverse transcription polymerase chain reaction (RT-PCR), 18 (90%) correctly determined serotype and 19 (95%) correctly identified CHIKV by RT-PCR. (who.int)

Typical stages in a tRNA (also termed class II) gene initiation: TFIIIC (Transcription Factor for polymerase III C) binds to two intragenic (lying within the transcribed DNA sequence) control sequences, the A and B Blocks (also termed box A and box B). TFIIIC acts as an assembly factor that positions TFIIIB to bind to DNA at a site centered approximately 26 base pairs upstream of the start site of transcription. (wikipedia.org)
The first protein that binds to all newly synthesized RNA polymerase III transcripts is a highly conserved phosphoprotein known as the La autoantigen. (yeastgenome.org)
In cytoplasm, zinc mostly binds zinc-chelating proteins called metallothioneins (MTs). (frontiersin.org)
In the interaction with proteins, lead binds with virtually every available functional group, including sulfhydryl, amine, phosphate, and carboxyl groups, with sulfhydryl having the highest affinity. (cdc.gov)
Lead also binds to metallothionein, a sulfhydryl-rich protein, but does not appear to displace cadmium or zinc. (cdc.gov)

Pol III is unusual (compared to Pol II) by requiring no control sequences upstream of the gene, instead normally relying on internal control sequences - sequences within the transcribed section of the gene (although upstream sequences are occasionally seen, e.g. (wikipedia.org)
In all cases, the process starts with transcription factors binding to control sequences, and ends with TFIIIB (Transcription Factor for polymerase III B) being recruited to the complex and assembling Pol III. (wikipedia.org)
The cumulative data obtained from using the various proteases (trypsin, chymotrypsin, Glu-C and Lys-C) and the two peptide fragmentation approaches allowed us to map nearly the complete sequences of all constituents of both Pol II and III. (cipsm.de)
This refers to species in which part of the sequence doubles back on itself and forms hydrogen-bonded bridges to complementary sequences on the same molecule and/or with other RNA molecules. (bioprocessintl.com)
The prediction models were trained on influenza protein sequences isolated from both avian and human samples, which were transformed into amino acid physicochemical properties feature vectors. (springer.com)
This strand has a circular chromosome (6.264 million base pairs long), that encodes 5,570 protein coding sequences. (kenyon.edu)

These rates were comparable to elongation rates of RNA polymerase II found by an in vivo study conducted on Drosophila. (wikipedia.org)
The analysis of the individual steps of RNA chain elongation depicted that adding U and A to U-terminated RNA chains was slow. (wikipedia.org)
Synthetic lethal interactions suggest a role for the Saccharomyces cerevisiae Rtf1 protein in transcription elongation. (embl-heidelberg.de)
Strong evidence indicates that transcription elongation by RNA polymerase II (pol II) is a highly regulated process. (embl-heidelberg.de)
Here we present genetic results that indicate a role for the Saccharomyces cerevisiae Rtf1 protein in transcription elongation. (embl-heidelberg.de)

nsp13 is an RNA helicase which is thought to melt secondary structures in the genomic RNA during replication and transcription. (reactome.org)

Studded in the lipid bilayer are two integral membrane proteins some 500 molecules of hemagglutinin ("H") and some 100 molecules of neuraminidase ("N"). Within the lipid bilayer are 3000 molecules of matrix protein and 8 pieces of RNA. (prospecbio.com)

1999). Cellular stress in Xenopus kidney cells enhances the phoshorylation of eukaryotic initiation factor eIF(4E) and the association of eIF4F with poly(A)-binding protein. (nature.com)
The multisubunit RNA polymerases (Pols) II and III synthesize mainly eukaryotic mRNAs and tRNAs, respectively. (cipsm.de)
Members of the conserved family of eukaryotic RNA-dependent RNA polymerases (Rdrs) synthesize double-stranded RNA (dsRNA) intermediates in diverse pathways of small RNA (sRNA) biogenesis and RNA-mediated silencing. (nih.gov)

We identified 21 meta-assemblages consisting of roughly 400 different proteins that are related to DNA replication, centromeres, subtelomeres, transposons, and transcription by RNA polymerase (Pol) I, II, and III. (genengnews.com)
Replication proteins engulf a nucleosome, centromeres lack a nucleosome, and repressive proteins encompass three nucleosomes at subtelomeric X-elements. (genengnews.com)
These proteins have been shown to play important roles in cell differentiation and organ development, and in regulating viral replication and transcription. (embl.de)

The genes transcribed by RNA Pol III fall in the category of "housekeeping" genes whose expression is required in all cell types and most environmental conditions. (wikipedia.org)
This leads to a high rate of transcriptional reinitiation of Pol III-transcribed genes. (wikipedia.org)
To identify YpdB-regulated genes, we compared the transcriptomes of E. coli cells overproducing either YpdB or a control protein. (cipsm.de)
However, the researchers found that "housekeeping" genes-which comprise the majority of genes in yeast-lacked a protein-DNA architecture that would allow specific transcription factors to bind, a hallmark of inducible genes. (genengnews.com)
The resolution and completeness of the data allowed us to identify 21 protein assemblages and also to identify the absence of specific regulatory control signals at housekeeping genes," concluded study co-author Shaun Mahony, PhD, an assistant professor of biochemistry and molecular biology at Penn State. (genengnews.com)
RNA polymerase, a protein complex responsible for transcribing genetic information, would normally stop reading this information at the end of the bacterial gene and would ignore any viral genes inserted behind it. (phys.org)
The virus uses a trick that prevents the RNA polymerase from terminating the transcription process: It introduces 'lambda-N' (λN), a tiny protein that attaches itself to the host 's RNA polymerase and forces it to continue transcription of the viral genes . (phys.org)
Because of this, the RNA polymerase continues transcribing even once it reaches the viral genes. (phys.org)
To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)

Although binding by the yeast La protein Lhp1p to pre-tRNAs is required for the normal pathway of tRNA maturation, the role of the La protein in the biogenesis of other polymerase III transcripts has been unclear. (yeastgenome.org)
Recessive mutations in POLR1C cause a leukodystrophy by impairing biogenesis of RNA polymerase III. (nih.gov)

TFIIIB remains bound to DNA following initiation of transcription by Pol III (unlike bacterial σ factors and most of the basal transcription factors for Pol II transcription). (wikipedia.org)
3D structure of the complex consisting of the bacterial RNA polymerase (light and dark gray) and the viral λN protein (red). (phys.org)
The nature of this structure told us that the small viral λN protein seals together the two halves of the RNA polymerase, thus preventing it from falling apart once it reaches the stop signal at the end of the bacterial gene," explains one of the study's first authors, Ferdinand Krupp, who is a doctoral student at Charité's Institute of Medical Physics and Biophysics. (phys.org)
The diagnosis of many infectious diseases, both viral and bacterial, may include the use of reverse transcriptase-polymerase chain reaction (RT-PCR). (medscape.com)

Iwr1 is important for Pol III transcription, because an iwr1 mutant strain shows reduced association of TBP and Pol III at Pol III promoters, a decreased rate of Pol III transcription, and lower steady-state levels of Pol III transcripts. (johnshopkins.edu)
1998) A role for the yeast La protein in U6 snRNP assembly: evidence that the La protein is a molecular chaperone for RNA polymerase III transcripts. (yeastgenome.org)
A perinucleolar compartment contains several RNA polymerase III transcripts as well as the polypyrimidine tract-binding protein, hnRNP I. (rupress.org)

Sequence and phylogenetic analyses scription-polymerase chain reaction (RT-PCR). (cdc.gov)
Initially, DNA is taken from the clinical specimen, as well as certain sequence-specific oligonucleotide primers, thermostable DNA polymerase, nucleotides, and buffer. (medscape.com)

Unprotected DNA is in lane 1, DNA protected by repressor protein is in lane 2, and RNA polymerase-protected DNA is in lane 3. (pearson.com)
A key mechanism appears to be the following: KRAB-AFPs tethered to DNA recruit, via their KRAB domain, the repressor KAP1 (KRAB-associated protein-1, also known as transcription intermediary factor 1 beta, KRAB-A interacting protein and tripartite motif protein 28). (embl.de)

Isolation and characterization of four novel parsley proteins that interact with the transcriptional regulators CPRF1 and CPRF2. (embl-heidelberg.de)
In the last decades, HOX proteins have been extensively studied due to their pivotal role in transcriptional events. (mdpi.com)
The functions currently known for members of the KRAB-containing protein family include transcriptional repression of RNA polymerase I, II and III promoters, binding and splicing of RNA, and control of nucleolus function. (embl.de)
The KAP1/ KRAB-AFP complex in turn recruits the heterochromatin protein 1 (HP1) family, and other chromatin modulating proteins, leading to transcriptional repression through heterochromatin formation. (embl.de)
Repression of transcriptional activity by heterologous KRAB domains present in zinc finger proteins. (embl.de)

There are three classes of Pol III initiation, corresponding to 5S rRNA, tRNA, and U6 snRNA initiation. (wikipedia.org)
We have shown previously that BAG-1 IRES-meditated initiation of translation requires two trans -acting factors poly (rC) binding protein 1 (PCBP1) and polypyrimidine tract binding protein (PTB) for function. (nature.com)

nsp14 also has the RNA capping activity and, in concert with nsp16, it caps viral plus strand and minus strand genomic and subgenomic RNAs, which confers stability to viral RNAs by enabling them to escape interferon-mediated innate immune responses of the host. (reactome.org)

Under stress conditions however, the protein Maf1 represses Pol III activity. (wikipedia.org)
This second project involved the protein Maf1, which inhibited Pol III activity in yeast and mammalian cells. (europa.eu)
The study of Maf1 could, therefore, reveal mechanisms by which the activities of the three Pols were regulated. (europa.eu)
Indeed, despite the importance of Maf1, the study of this protein in mammalian cells was just beginning by the time of the project elaboration. (europa.eu)
Secondly, we identified important aspects of the regulation of the Pol III inhibitor Maf1. (europa.eu)

Reduced function of the RNA polymerase III molecule likely affects development and function of many parts of the body, but how the POLR3A gene mutations result in the specific signs and symptoms of Wiedemann-Rautenstrauch syndrome is unknown. (medlineplus.gov)

In eukaryote cells, RNA polymerase III (also called Pol III) is a protein that transcribes DNA to synthesize 5S ribosomal RNA, tRNA and other small RNAs. (wikipedia.org)

2006). Polypyrimidine tract binding protein regulates IRES-mediated gene expression during apoptosis. (nature.com)

In our analysis of Pol II and III we detected 19 phosphorylation sites, of which 12 have not been previously reported. (cipsm.de)
The complex regulates histone H2B ubiquitination, histone H3 methylation, RNA polymerase II carboxy-terminal domain (CTD) Ser2 phosphorylation, and RNA 3' end processing. (embl-heidelberg.de)

It contains only a limited number of highly conserved amino acids, among which are three positively charged residues that gave the Plus3 domain its name. (embl-heidelberg.de)

Two-hybrid screens using CPRF1 and CPRF2 as "baits" resulted in the isolation of four novel parsley proteins which interact with either CPRF1 or CPRF2 in vivo. (embl-heidelberg.de)

Jordanian H1N1 viruses had mutations that are characteristic of antigenic group 6 while H3N2 virus mutations belonged to group 3. (who.int)
Peu de renseignements sont disponibles sur les mutations des virus saisonniers de la grippe A(H1N1)pdm09 et H3N2 en Jordanie. (who.int)
Les virus H1N1 jordaniens présentaient des mutations caractéristiques du groupe antigénique 6, tandis que les virus H3N2 appartenaient au groupe 3. (who.int)
Les mutations individuelles sont décrites en détail. (who.int)

Information about cross-species functional complementation between yeast and other species, curated by SGD and the Princeton Protein Orthology Database (P-POD). (yeastgenome.org)
However, in collaboration with Drs Ingrid Grummt and Holger Bierhoff from the German Cancer Research Center , Heidelberg, Germany, I found that the mechanism by which Pol I synthesised ribosomal RNA in human cells was different enough from the mechanism used in yeast cells, therefore I could not use the previously exploited approach to deregulate Pol I activity in yeast. (europa.eu)
Here we analyzed both yeast Pol II and Pol III by multiplexed mass spectrometric analysis using various proteases and both collision induced and electron transfer dissociation. (cipsm.de)
No yeast two-hybrid interactions found for this protein. (yeastrc.org)

Therefore, the regulation of Pol III transcription is primarily tied to the regulation of cell growth and the cell cycle, and thus requires fewer regulatory proteins than RNA polymerase II. (wikipedia.org)
At its upper end, the λN protein is in contact with RNA (orange) and various regulatory proteins (yellow, blue and green). (phys.org)

We are currently experiencing an unprecedented COVID-19 pandemic caused by a novel RNA coronavirus called SARS-CoV-2, which can produce a severe acute respiratory distress syndrome (ARDS) ( 13 ). (frontiersin.org)
It was named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), causing coronavirus disease 2019 (COVID-19) (2,3). (who.int)

Purity among commercially available polymerase enzymes is variable, and some enzymes are stabilized with bovine serum albumin (BSA). (bioprocessintl.com)

Finally, nucleotides complementary to the target DNA are added extending each primer by the thermostable DNA polymerase. (medscape.com)

Ribosomes consist of ribonucleic acids (RNAs) and proteins. (europa.eu)

Thus, the making of a ribosome requires the action of three different Pols. (europa.eu)
It is believed that, in order to obtain a balanced amount of ribosomal components, the activities of the three Pols are co-regulated. (europa.eu)

Currently, there are three basic types of tests to determine if an individual has been infected with SARS-CoV-2: viral nucleic acid (RNA) detection, viral antigen detection, and detection of antibodies to the virus. (medscape.com)
Module A contained serum samples spiked with cultured dengue virus (DENV) or chikungunya virus (CHIKV) for the detection of nucleic acid and DENV non-structural protein 1 (NS1) antigen. (who.int)

4. Translated into viral proteins that are used to make the virus envelope. (flashcardmachine.com)
While it is routine to detect structural viral proteins in vitro for diagnostic purposes, it previously remained impossible to directly and dynamically visualize HIV in living cells without genetic modification of the virus. (cipsm.de)

A concomitant decrease in Ataxin-3 activity facilitates CBP ubiquitination and degradation, adversely impacting transcription and DNA repair. (elifesciences.org)

TFIIIB is the transcription factor that assembles Pol III at the start site of transcription. (wikipedia.org)

2002). Translation of polioviral mRNA is inhibited by cleavage of polypyrimidine tract-binding proteins executed by polioviral 3C(pro). (nature.com)
Thus, RNA polymerase copies information from template DNA to generate the mRNA template for protein production. (bham.ac.uk)

Posttranscriptional variants include subpopulations of double-stranded RNA (dsRNA). (bioprocessintl.com)

Plus3 domains occur in the Saccharomyces cerevisiae Rtf1p protein, which interacts with Spt6p, and in parsley CIP, which interacts with the bZIP protein CPRF1. (embl-heidelberg.de)

The N-terminal helices alpha1-alpha3 and C-terminal helix alpha6 pack together to form an alpha subdomain, while the beta strands and the small 3(10) helix alpha 4 form a beta subdomain. (embl-heidelberg.de)

The influenza virus's hemagglutinin (HA) surface proteins then bind to the sialic acid receptors on the surface of a human respiratory tract cell. (cdc.gov)
The structure of the influenza virus's HA surface proteins is designed to fit the sialic acid receptors of the human cell, like a key to a lock. (cdc.gov)
The above image shows the different features of an influenza virus, including the surface proteins hemagglutinin (HA) and neuraminidase (NA). (cdc.gov)
Following influenza infection or receipt of the influenza vaccine, the body's immune system develops antibodies that recognize and bind to "antigenic sites," which are regions found on an influenza virus' surface proteins. (cdc.gov)
The virus' hemagglutinin (HA) and neuraminidase (NA) surface proteins are displayed in semi-transparent blue sticking out of the surface of the virus. (cdc.gov)
Three of these RNP segments encode the virus' surface proteins, i.e., the HA, NA and M proteins. (cdc.gov)

Cells need ribosomes in order to create proteins. (europa.eu)

We discuss the possible roles of the newly identified proteins in CPRF1- and CPRF2-dependent target gene expression. (embl-heidelberg.de)

There are three major isoforms of BAG-1 in mammalian cells, termed BAG-1L (p50), BAG-1M (p46) and BAG-1S (p36) that function as pro-survival proteins and are associated with tumorigenesis and chemoresistance. (nature.com)

For example, complex regulation of expression of the nucleoid-associated protein cbpA was found to be dependent not only on sigma factor specificity, but also on binding of the transcription factor Fis upstream of the cbpA start codon. (bham.ac.uk)

However, the use of cell culture to produce recombinant proteins is still susceptible to contamination with viruses. (researchgate.net)
Understanding and predicting host tropism of influenza proteins lay an important foundation for future work in constructing computation models capable of directly predicting interspecies transmission of influenza viruses. (springer.com)

It must be absent from a final product to prevent triggering a toxic cytokine storm and to facilitate proper translation of the single-stranded RNA (ssRNA) ( 1 ). (bioprocessintl.com)
The ATPase activities of both proteins are stimulated by single-stranded DNA. (mpg.de)

[ 1 ] The resultant complementary DNA is amplified by polymerase chain reaction (PCR). (medscape.com)

1996). HGF receptor associates with the anti-apoptotic protein BAG-1 and prevents cell death. (nature.com)

Background: Iwr1, a protein conserved throughout eukaryotes, was originally identified by its physical interaction with RNA polymerase (Pol) II. (johnshopkins.edu)
In Eukaryotes, tRNAs, 5S RNA and U6 RNA are transcribed by RNA polymerase (Pol) III. (mpg.de)

These results provide evidence that Lhp1p is a molecular chaperone for polymerase III-transcribed RNAs and implicate Lsm8p as a key component in the very early steps of U6 snRNP assembly. (yeastgenome.org)
3 Institute of Microbiology and Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, v.v.i. (xray.cz)

It is the presence of a TATA box that specifies that the snRNA gene is transcribed by Pol III rather than Pol II. (wikipedia.org)

The described multiplexed proteomics approach is generic and reveals that it is possible to map a protein complex to near completion while applying less than 5 μg (approximately 10 pmol) of total starting material. (cipsm.de)
Until now, and despite intensive efforts, researchers had failed to identify how this tiny protein can achieve such a feat. A Berlin-based team of researchers has now been able to visualize the 3-D structure of the RNA polymerase-λN-complex using high-resolution imaging, enabling them to provide a detailed explanation of this viral exploitation. (phys.org)
They started by producing the individual components of this large protein complex separately. (phys.org)
Using cryo- electron microscopy , the researchers took a total of 700,000 images of the protein complex from various angles, using these to compute its 3-D structure. (phys.org)
No complex found for this protein. (yeastrc.org)
Here we show that all three sites are required for NorR-dependent catalysis of open complex formation by 54-RNAP holoenzyme (E54). (strath.ac.uk)
The RNPs were shown in white, with their coiled structures, and three-bulbed polymerase complex on the ends. (cdc.gov)

Anatomy8

Organisms16

Diseases2

Chemicals and Drugs120

Analytical, Diagnostic and Therapeutic Techniques and Equipment21

Phenomena and Processes73

Information Science3

Health Care2

Subunit7

Subunits9

Synthesis10

Genome9

Promoter7

SARS-CoV RNA2

Reverse transcri2

Binds5

Sequences6

Elongation5

Helicase1

Molecules1

Eukaryotic3

Replication3

Genes9

Biogenesis2

Bacterial4

Transcripts3

Sequence2

Repressor2

Transcriptional5

Initiation2

Genomic1

Maf15

Molecule1

Synthesize1

Polypyrimidine tract b1

Phosphorylation2

Amino acids1

Vivo1

Mutations4

Yeast4

Regulatory2

Coronavirus2

Commercially available1

Nucleotides1

Ribonucleic1

Pols2

Nucleic acid2

Viral proteins2

Ubiquitination1

Assembles1

MRNA2

DsRNA1

Saccharomyces1

Helices1

Surface Proteins6

Ribosomes1

Gene expression1

Mammalian1

Transcription factor1

Viruses2

Single-stranded2

Chain reaction1

Receptor1

Eukaryotes2

Molecular2

SnRNA1

Complex7