• miRNAs are a family of small non-coding RNAs that emerged as significant and versatile regulators involved in regulation of immune responses. (frontiersin.org)
  • MicroRNAs (miRNAs) are highly conserved, single-stranded, small non-coding RNAs that have been shown to act as essential regulators of gene expression at the posttranscriptional level. (frontiersin.org)
  • MicroRNAs (miRNAs) are small, endogenous, non-coding RNAs, which act as key post-transcriptional regulators of target-gene expression. (spandidos-publications.com)
  • 1,000 human miRNAs have been identified that regulate ~1/3 of the coding genes in the human genome ( 9 ). (spandidos-publications.com)
  • Individual miRNAs are capable of regulating gene networks by interacting with multiple targets or by suppressing transcriptional controllers [ 6 , 8 ]. (researchsquare.com)
  • miRNAs are essential for brain development and function, with multiple miRNAs enriched in specific cell types where they regulate differentiation, structure and neurophysiological properties [ 9 , 10 ]. (researchsquare.com)
  • Expression of various miRNAs is dysregulated in experimental models of status epilepticus and in resected brain tissue from patients with drug-resistant temporal lobe epilepsy [ 11-13 ]. (researchsquare.com)
  • MicroRNAs (miRNAs) are short (21-24 nucleotides) sequences of RNA that reduce gene expression by promoting the breakdown of messenger RNAs (mRNAs) and by repressing translation of mRNAs into proteins. (freethoughtblogs.com)
  • We show that, similar to metazoan steady-state systems, endogenous miRNAs in Chlamydomonas can regulate gene expression both by destabilization of the mRNA and by translational repression. (freethoughtblogs.com)
  • Objective: To identify miRNAs that regulate angiogenesis. (ca.gov)
  • The test uses nanoparticles to capture tumor-specific microRNAs (miRNAs) in human serum. (mordorintelligence.com)
  • that asthma is intensively regulated by a variety of microRNAs (miRNAs). (cdc.gov)
  • MiRNAs are approximately 19- to 25-nucleotide estimated about 250,000 and mortality does not appear single-stranded, noncoding RNAs that exist in both to correlate well with prevalence. (cdc.gov)
  • As binding of miRNAs to a target mRNA may result in an airway inflammatory disease closely correlates with either mRNA degradation or inhibition of protein immune regulation, more and more evidences suggest translation.1 More than 2000 miRNAs have been discovered in humans. (cdc.gov)
  • Th)1 cells.5 Meanwhile, the function and immunity of with genetic predisposition, involving multiple cells, DCs are closely regulated by miRNAs. (cdc.gov)
  • Small noncoding RNAs called microRNAs (miRNA) negatively regulate gene expression by sequence-specific targeting of protein-coding transcripts [ 6 ]. (researchsquare.com)
  • Binding of a miRNA to a complementary sequence in a target mRNA is mediated by Argonaute proteins leading to degradation of the mRNA or translational inhibition, thereby lowering protein levels in cells [ 7 ]. (researchsquare.com)
  • Here, we provide a comprehensive global analysis of the evolutionarily distant unicellular green alga Chlamydomonas reinhardtii to quantify the effects of miRNA on protein synthesis and RNA abundance. (freethoughtblogs.com)
  • The nature of imperfect binding specificity means that a single miRNA can regulate a large number of mRNA targets involved in complex cellular processes, thereby tightly controlling genetic networks during development and in response to stress ( Pocock, 2011 ). (elifesciences.org)
  • Autophagy is a degradation system that involves sequestration of cytoplasmic proteins and organelles by double-layered membranes that form vesicles called autophagosomes. (elifesciences.org)
  • Fusion of autophagosomes with lysosomes results in degradation of their contents and thereby removes toxic proteins and damaged organelles from cells to maintain homeostasis. (elifesciences.org)
  • Degradation of cellular proteins in the ubiquitin-proteasome system regulates various biological processes. (helsinki.fi)
  • MicroRNA‑130a (miR‑130a) has previously been reported to promote cervical cancer growth. (spandidos-publications.com)
  • High expression of miR‑130a was significantly associated with lymph node metastasis and an advanced clinical stage of cervical cancer. (spandidos-publications.com)
  • Furthermore, the expression of miR‑130a was also higher in HPV(+) cervical cancer cell lines compared with that in HPV(‑) cells. (spandidos-publications.com)
  • Knockdown of HPV18 E6 significantly inhibited the expression of miR‑130a in HeLa cervical cancer cells. (spandidos-publications.com)
  • In summary, the present study suggests that HPV18 E6 promotes the expression of miR‑130a, which further inhibits the expression of TIMP2 and promotes cervical cancer cell invasion. (spandidos-publications.com)
  • Zhang JJ, Wang DD, Du CX and Wang Y: Long noncoding RNA ANRIL promotes cervical cancer development by acting as a sponge of miR-186. (spandidos-publications.com)
  • Yang M, Zhai X, Ge T, Yang C and Lou G: MiR-181a-5p promotes proliferation and invasion, and inhibits apoptosis of cervical cancer cells via regulating inositol polyphosphate-5-phosphatase A (INPP5A). (spandidos-publications.com)
  • Gao YL, Zhao ZS, Zhang MY, Han LJ, Dong YJ and Xu B: Long noncoding RNA PVT1 facilitates cervical cancer progression via negative regulating of miR-424. (spandidos-publications.com)
  • Circular RNA circSDHC (hsa_circ_0015004) regulates tumor growth and angiogenesis via regulating centrosomal protein 55 expression in renal cell carcinoma. (bvsalud.org)
  • Rationale: Formation and remodeling of the vasculature during development and disease involves a highly conserved and precisely regulated network of attractants and repellants. (ca.gov)
  • Methods and Results: We show that the highly conserved microRNA family encoding miR-10 regulates the behavior of endothelial cells during angiogenesis by positively titrating pro-angiogenic signaling. (ca.gov)
  • Finally, IGF‑1R protein, phosphoinositide 3‑kinase/Akt signaling pathway‑associated proteins and cyclin pathway‑associated proteins were differentially expressed between miR‑497‑overexpressing cells and miR-497-silenced cells. (spandidos-publications.com)
  • However, whether miR-497 regulates other target genes in HCC is unknown. (spandidos-publications.com)
  • Using RNA-Seq, we identified a total of 163 and 568 differentially expressed genes in primary normal human bronchial epithelial (NHBE) cells that were exposed to diacetyl and 2,3-pentanedione, respectively. (nature.com)
  • Consistent with this, qRT-PCR confirmed that the expression of multiple genes involved in cilia biogenesis was significantly downregulated by diacetyl and 2,3-pentanedione in NHBE cells. (nature.com)
  • The immune-related DEOSGs and hub genes were identified by weighted gene co-expression network analysis (WGCNA) and protein-protein interaction (PPI) analysis, respectively. (nature.com)
  • The mRNA expression of diagnostic genes was determined by qRT-PCR analysis. (nature.com)
  • Finally, we constructed the drug, transcription factors (TFs), and microRNA network of the diagnostic genes. (nature.com)
  • Thus RUNX1/ETO contributes to increased expression of miR451 target genes and interferes with normal gene expression during differentiation. (uni-frankfurt.de)
  • The role of miR‑497 in regulating the proliferation of HCC‑derived cells was also investigated in vitro and in vivo. (spandidos-publications.com)
  • Zhu K, He Y, Xia C, Yan J, Hou J, Kong D, Yang Y and Zheng G: MicroRNA-15a inhibits proliferation and induces apoptosis in CNE1 nasopharyngeal carcinoma cells. (spandidos-publications.com)
  • Zhou Y, Yang C, Wang K, Liu X and Liu Q: MicroRNA-33b inhibits the proliferation and migration of osteosarcoma cells via targeting hypoxia-inducible factor-1α. (spandidos-publications.com)
  • p class=\'abstract\'>Lin28/LIN-28 is a conserved RNA-binding protein that promotes proliferation and pluripotency and can be oncogenic in mammals. (stanford.edu)
  • The expression of TIMP2 was negatively mediated by miR‑130a, and HPV18 E6 inhibited the expression of TIMP2 in HeLa cells. (spandidos-publications.com)
  • We also identified a novel mRNA processing mechanism that expands human proteome at the posttranscriptional level and regulates gene expression (Yao, P, et al. (rochester.edu)
  • Following recognition of TLR ligands, TLRs elicit innate immunity by activating multiple intracellular signaling cascades including variable adaptor proteins and transcriptional factors. (frontiersin.org)
  • MicroRNAs perform important roles in the post-transcriptional regulation of gene expression. (researchsquare.com)
  • A number of post-transcriptional mechanisms fine-tune the gene expression landscape. (researchsquare.com)
  • HCK expression is up-regulated at the transcriptional level during myeloid differentiation of hematopoietic cells. (uni-frankfurt.de)
  • Transcription factors (TFs) are critical for B-cell differentiation, affecting gene expression both by repres- sion and transcriptional activation. (lu.se)
  • C. Elegans LIN-28 Controls Temporal Cell Fate Progression by Regulating LIN-46 Expression via the 5' UTR of Lin-46 MRNA. (stanford.edu)
  • pubMedId':'34496246','pubMedUrl':'https://www.ncbi.nlm.nih.gov/pubmed/34496246','publicationId':'826388','title':'C. elegans LIN-28 controls temporal cell fate progression by regulating LIN-46 expression via the 5' UTR of lin-46 mRNA. (stanford.edu)
  • Silencing of circSDHC restrained cell malignant phenotypes and angiogenesis via reducing CEP55 expression by releasing miR-130a-3p in RCC, providing a new mechanism for understanding the progression of RCC. (bvsalud.org)
  • Lin28/LIN-28 also bind and regulate many mRNAs in diverse cell types. (stanford.edu)
  • Although more and animals and plants and regulate gene/protein expression more progressions have been made about the through direct complementarity between their 5' region pathogenesis of asthma in recent years, the increasing and the 3' untranslated region of target mRNAs. (cdc.gov)
  • During his post-doctoral training under Dr. Paul Fox at Lerner Research Institute of Cleveland Clinic, he made contributions in understanding the translational control mechanisms regulating VEGFA (vascular endothelial growth factor-A) expression in human monocytes. (rochester.edu)
  • Thus, to maintain a balance between host preservation and inflammatory pathology, the immune response and inflammation need to be tightly regulated. (frontiersin.org)
  • Abstract: A network of lineage-specific transcription factors and microRNAs tightly regulates differentiation of hematopoietic stem cells along the distinct lineages. (uni-frankfurt.de)
  • Regulatory non-coding RNAs and RNA-binding proteins (RBPs) are important research areas in gene regulation and RNA biology. (rochester.edu)
  • Our laboratory is interested in the understanding of pathophysiological function and molecular mechanism of new non-coding RNAs (and RBPs) and new modes of gene regulation in c. (rochester.edu)
  • Although glucocorticoid receptors (GRs) in the hippocampus play a vital role in the regulation of physiological and behavioural responses to stress, the regulation of receptor expression remains unclear. (karger.com)
  • We have only known that microRNAs even existed since the early 1990's, and their importance in gene regulation and development wasn't appreciated until the 2000's. (freethoughtblogs.com)
  • The objective of this study is to identify microRNAs-small, non-coding RNAs that help control gene expression-that contribute to the regulation of new blood vessel formation. (ca.gov)
  • Appropriate regulation of autophagy is crucial for clearing toxic proteins from cells. (elifesciences.org)
  • Our results show that Sp1 plays an essential role in the regulation of the differential gene expression of the HCKgene. (uni-frankfurt.de)
  • MicroRNAs participate in the regulation of asthma, the goal of this study is to summarize recent researches on the roles of microRNAs in the pathogenesis of asthma. (cdc.gov)
  • MicroRNAs not only participate in determining DCs phenotype and then naive T lymphocyte differentiation, but also participate in the regulation of airway inflammation and airway remodeling in asthma. (cdc.gov)
  • Zhang et al cytokines, mediators and signals and closely related to demonstrated that microRNA let-7i was upregulated immune regulation. (cdc.gov)
  • however, the expression, function and underlying mechanisms of miR‑497 in HCC remain unclear. (spandidos-publications.com)
  • The long-term objective of our laboratory is to identify novel RNA-based molecular mechanisms that control gene expression and conduct pathophysiological function in cardiac system, as well as to develop novel therapeutic approaches for the prevention or treatment of human cardiovascular diseases. (rochester.edu)
  • Due to the central role of autophagy in the removal of aggregation-prone proteins, a better understanding of mechanisms controlling autophagy is essential for the identification of novel therapeutic opportunities for multiple disease states. (elifesciences.org)
  • Rat hippocampi were micro-dissected (for mRNA, microRNA, and DNA methylation analysis) or frozen whole (for chromatin immunoprecipitation). (karger.com)
  • This was related to an increased DNMT3a protein association with a discreet region of the Nr3c1 (GR gene) promoter, shown here to undergo increased DNA methylation after FSS. (karger.com)
  • Sequencing as well as functional studies using antisense oligonucleotides, indicate important roles for microRNAs during the development of epilepsy through targeting transcripts involved in neuronal structure, gliosis and inflammation. (researchsquare.com)
  • We found that the hematopoietic master regulator RUNX1 controls the expression of certain microRNAs, of importance during erythroid/megakaryocytic differentiation. (uni-frankfurt.de)
  • RUNX1 thus emerges as a key regulator of a microRNA network, driving differentiation at the megakaryocytic/erythroid branching point. (uni-frankfurt.de)
  • Mammalian Lin28 and C. elegans LIN-28 have been shown to inhibit biogenesis of the conserved cellular differentiation-promoting microRNA let-7 by directly binding to unprocessed let-7 transcripts. (stanford.edu)
  • Protein synthesis, folding, and tertiary and quaternary structure ultimately determine much of the body's structure and function. (msdmanuals.com)
  • This gene is a member of the protein-tyrosine kinase oncogene family. (cancerindex.org)
  • We found that miR-10 functions, in part, by directly regulating the level of fms-related tyrosine kinase 1 (FLT1), a cell-surface protein that sequesters VEGF, and its soluble splice variant sFLT1. (ca.gov)
  • Expression of a reporter gene linked to the −101HCK promoter region was up-regulated by Sp1, but not by other members of the Sp1 family of transcription factors, in Drosophila Schneider cells. (uni-frankfurt.de)
  • The identified transcription factors influence both the global and specific gene expression of the BCLs and have possible implications for diagnosis and treat- ment. (lu.se)
  • Circular RNAs have been shown to exert critical roles in RCC. (bvsalud.org)
  • The results of the present study indicate that microRNAs play important roles in regulating asthma immune responses. (cdc.gov)
  • He received his Ph.D. degree in the laboratory of Dr. En-Duo Wang in Biochemistry and Molecular Biology from Shanghai Institute of Biochemistry and Cell Biology of Chinese Academy of Science in 2008, where he studied the structural and functional interplay of transfer RNA (tRNA) and tRNA synthetase in bacteria, yeast and human. (rochester.edu)
  • A gene, the basic unit of heredity, is a segment of DNA containing all the information necessary to synthesize a polypeptide (protein) or a functional RNA molecule. (msdmanuals.com)
  • Therefore, the present study investigated miR‑497 expression in HCC samples and HCC‑derived cell lines using reverse transcription‑quantitative polymerase chain reaction. (spandidos-publications.com)
  • Expression changes of circSDHC were determined by real- time quantitative polymerase chain reaction (RT-qPCR). (bvsalud.org)
  • Western blotting was applied to investigate the protein phosphorylation level (i.e. activation) of AMPK. (helsinki.fi)
  • Genetic experiments in zebrafish revealed that miR-10 functions, in part, by directly regulating the level of a protein called FLT1, which inhibits the behavior of endothelial cells that promotes new blood vessel growth. (ca.gov)
  • Intriguingly, some of these RNA switches involve the interplay between microRNA and RNA-binding protein (Jafarifar, F, et al. (rochester.edu)
  • FSS also caused a time-dependent increase in the expression of miR-124a, a microRNA known to reduce GR mRNA expression, which was inversely correlated with a reduction in GR mRNA levels 30 min after FSS. (karger.com)
  • Acute stress results in decreased GR mRNA expression specifically in the dentate gyrus. (karger.com)
  • This is predicted to exert an important influence on the gene expression landscape. (researchsquare.com)
  • The protein expression of one of the predicted common targets of miR‑497, insulin‑like growth factor‑1 receptor (IGF‑1R), was assessed using western blot analyses and immunohistochemistry. (spandidos-publications.com)
  • Over-expression of miR-22 has been reported to protect in models of brain injury and additional targets of miR-22 have been identified [ 17 , 18 ]. (researchsquare.com)
  • Our previous study has shown that CCAAT-enhancer binding protein β (C/EBPβ) is an important regulator in METH-induced neuronal autophagy and apoptosis. (cancerindex.org)
  • The ability to regulate blood vessel growth is important for treating many diseases, including cancer. (ca.gov)
  • Pathologic features of AD are characterized by persistent deposition of the intercellular amyloid β-peptide (Aβ) plaques and intracellular tau protein and impairment of the neuron-to-neuron synaptic communication and nutrient transportation inside neuron 3 . (nature.com)
  • To elucidate the molecular basis of the differential HCK gene expression, the genomic region containing the HCK promoter was isolated and functionally characterized. (uni-frankfurt.de)
  • Using RNA-Seq, we performed global transcriptomic profiling in primary NHBE cells exposed to diacetyl or 2,3-pentanedione. (nature.com)
  • We recently discovered a new type of stress-responsive, protein-directed human RNA switch that regulates expression of vascular endothelial growth factor-A in human monocytic cells (Ray, PS, et al. (rochester.edu)
  • We also measured the levels of phosphorylated tuberous sclerosis complex 2 (TSC2) protein and Parkin protein level in SH-SY5Y cells. (cancerindex.org)
  • miR-10 Regulates the Angiogenic Behavior of Zebrafish and Human Endothelial Cells by Promoting VEGF Signaling. (ca.gov)
  • We show that a microRNA called miR-10 regulates the behavior of endothelial cells during blood vessel formation by encouraging signals that promote blood vessel growth. (ca.gov)
  • The increase in FLT1/sFLT1 protein levels upon miR-10 knockdown in zebrafish and in HUVECs inhibited the angiogenic behavior of endothelial cells largely by antagonizing VEGF receptor-2 signaling. (ca.gov)
  • Here, we report that the microRNA miR-1 regulates the autophagy pathway through conserved targeting of the orthologous T re-2/ B ub2/ C DC16 (TBC) Rab GTPase-activating proteins TBC-7 and TBC1D15 in Caenorhabditis elegans and mammalian cells, respectively. (elifesciences.org)
  • Further, we found that the cytokine interferon-β (IFN-β) can induce miR-1 expression in mammalian cells, reducing TBC1D15 levels, and safeguarding against proteotoxic challenges. (elifesciences.org)
  • The human hemopoietic cell kinase (HCK) is a member of the src family of protein tyrosine kinases specifically expressed in myeloid cells and to a minor extent in B-lymphoid cells. (uni-frankfurt.de)
  • Electrophoretic mobility shift analysis using nuclear extracts obtained from RAW264 cells and from the promonocytic cell line U-937 revealed the formation of at least three distinct protein-DNA complexes at each of these sites, one of which was found to contain the transcription factor Sp1. (uni-frankfurt.de)
  • Upregulated miR-21 suppresses LPS-induced inflammatory cytokine expression by targeting IL-1 receptor-associated kinase 4 (IRAK4), thereby avoiding excessive inflammatory responses. (frontiersin.org)
  • In January 2021, Diagenode launched a new small RNA-sequencing solution, D-Plex Small RNA DNBSEQ Kit, compatible with MGI's DNBSEQ sequencers. (mordorintelligence.com)
  • The kit incorporates unique D-Plex technology for library preparation enabling high performance with ultralow RNA inputs and providing diverse small RNA transcript detection. (mordorintelligence.com)
  • Recently, an increasing number of studies have revealed that microRNA-497 (miR-497) levels are decreased in tumors, and that it functions as a tumor suppressor in a number of types of human cancer, including colorectal, gastric, cervical and breast cancers, adrenocortical carcinoma and melanoma ( 12 - 20 ). (spandidos-publications.com)
  • In the last decade, diagnostic and prognostic evaluation has been facilitated by global gene expression profiling (GEP), provid- ing a new powerful means for the classification, prediction of survival, and response to treatment of lym- phomas. (lu.se)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • Moreover, we find that precocious LIN-46 expression caused by mutations in the lin-46 5' UTR is sufficient to cause precocious heterochronic defects similar to those of lin-28(lf) animals. (stanford.edu)
  • RNA sequencing analysis of the hippocampus during the period of epileptogenesis revealed a specific suppression of inflammatory signalling in the hippocampus of miR-22-deficient mice. (researchsquare.com)
  • Formation and remodeling of blood vessels during development and disease involves a precisely regulated network of attractants and repellants that is similar across species. (ca.gov)
  • Search the gene expression profiles from curated DataSets in the Gene Expression Omnibus (GEO) repository. (cancerindex.org)
  • Western blotting and immunohistochemical analyses revealed that IGF‑1R expression was significantly increased in HCC compared to control tissues. (spandidos-publications.com)
  • We observed an observable elevation in circSDHC expression in RCC tissues and cell lines . (bvsalud.org)
  • We found that lipopolysaccharide and Vibrio anguillarum significantly upregulated the expression of fish miR-21. (frontiersin.org)
  • In the present study, polymerase chain reaction and western blot analyses were performed to examine the expression levels of miR‑130a and associated proteins. (spandidos-publications.com)
  • Remarkably, already undamaged oocytes express high levels of the protein, suggesting that TAp63a's activity is under tight control of an inhibitory mechanism. (uni-frankfurt.de)
  • Our results demonstrate how TAp63a is inhibited by complex domain-domain interactions that provide the basis for regulating quality control in oocytes. (uni-frankfurt.de)
  • Defective autophagy results in accumulation of toxic protein aggregates that detrimentally affect cellular function and organismal survival. (elifesciences.org)
  • MicroRNA-22 (miR-22) has been reported to protect against the development of epileptogenic brain networks through suppression of neuroinflammatory signalling. (researchsquare.com)