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Repressor ProteinsProtein BindingMolecular Sequence DataProtein Interaction MappingOperator Regions, GeneticTranscription FactorsTwo-Hybrid System TechniquesBinding SitesLac RepressorsAmino Acid SequenceBase SequenceProtein Structure, TertiaryModels, MolecularProtein ConformationDNA-Binding ProteinsBacterial ProteinsOperonIsopropyl ThiogalactosideTranscription, GeneticEscherichia coliPromoter Regions, GeneticCell LineViral Regulatory and Accessory ProteinsGene Expression Regulation, BacterialGenes, RegulatorPlasmidsMutationEscherichia coli ProteinsDNA, BacterialDNALysogenyLac OperonZinc FingersGenes, BacterialBacteriophage lambdaCloning, MolecularGene Expression RegulationRecombinant Fusion ProteinsNuclear ProteinsLactoseDNA FootprintingHistone DeacetylasesEnzyme RepressionViral ProteinsTrans-ActivatorsRegulatory Sequences, Nucleic AcidTetracyclineRNA, MessengerElectrophoretic Mobility Shift AssayRestriction MappingColiphagesConsensus SequenceDeoxyribonuclease IGenes, ReporterDimerizationProtein BiosynthesisCarrier ProteinsDrosophila ProteinsSequence Homology, Amino AcidMutagenesis, Site-Directedbeta-GalactosidaseGene Expression Regulation, FungalTransfectionBasic Helix-Loop-Helix Transcription FactorsSequence AlignmentHomeodomain ProteinsGene Expression Regulation, DevelopmentalNucleic Acid ConformationCell NucleusRecombinant ProteinsHeLa CellsModels, GeneticSaccharomyces cerevisiaeKineticsTranscriptional ActivationSaccharomyces cerevisiae ProteinsRNA-Binding ProteinsGene SilencingSequence Homology, Nucleic AcidAlcohol OxidoreductasesDrosophilaTryptophanBacillus subtilisGenetic Complementation TestArabidopsis ProteinsDNA Transposable ElementsChloramphenicol O-AcetyltransferaseInsect ProteinsMutagenesisSignal TransductionDNA, ViralGenesPhenotypeCyclic AMP Response Element ModulatorModels, BiologicalStructure-Activity RelationshipAllosteric RegulationSequence DeletionDNA PrimersGene Expression
Transcriptional repressorGenesStructural ClassifiMetabolismMoleculeBindingInhibitBacterialHistoneDimerizationBindsDrosophilaSpeciesHomologRecombinantInteractsHomeoticFunctionalSequenceNeuronsColiSynthesisComplexFunctionExpressionStructureSerineRegulatorSimilaritiesPhysicalSubunitRecognitionPrimarilyResistanceRepeatsRegulationMRNASpecific
Transcriptional repressor5
  • The Drosophila extra sex combs (esc) protein, a member of the Polycomb group (PcG), is a transcriptional repressor of homeotic genes. (umn.edu)
  • In this respect, the transcriptional repressor PARIS (ZNF746) was identified by our group in 2011 6 among several other co-substrates of PINK1 and parkin as a promising candidate to shed light on possible contributions of a defective mitochondrial biogenesis to PD pathogenesis. (nature.com)
  • Transcriptional repressor. (antikoerper-online.de)
  • Transcriptional repressor during pronephros development. (xenbase.org)
  • can function as a transcriptional repressor. (atlasgeneticsoncology.org)
Genes8
  • In Rosetta(DE3)pLacI, the rare tRNA genes are present on the same plasmid that carries the lac repressor gene. (sigmaaldrich.com)
  • Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. (sigmaaldrich.com)
  • A transcriptional co-repressor is a protein that interacts with other DNA-binding proteins to suppress the activity of certain genes, although it cannot attach (bind) to DNA by itself. (medlineplus.gov)
  • Each repressor targets a specific co-regulated group of genes by recognizing a specific sequence of DNA, called the operator in bacteria . (proteopedia.org)
  • Repressor proteins are coded for by regulatory genes. (proteopedia.org)
  • The lac repressor, and the group of genes it controls, which is called an operon , were the first such gene regulatory system to be discovered. (proteopedia.org)
  • AcrR is a one-component allosteric repressor of the genes associated with lipid transport and antibiotic resistance. (rcsb.org)
  • Transcription factor Nrf2 is a key regulator of genes encoding phase II detoxification enzymes and antioxidant proteins in response to environmental chemical insults. (cdc.gov)
Structural Classifi2
Metabolism2
  • Originally discovered as a chief regulatory protein of glucose and lipid metabolism and cell differentiation, the peroxisome proliferator-activated receptor gamma (PPARγ) is a ligand-activated transcription factor of the nuclear hormone receptor superfamily 8 . (nature.com)
  • The lactose ("lac") repressor controls the expression of bacterial enzymes involved in the metabolism of of the sugar lactose. (proteopedia.org)
Molecule3
  • For a general introduction to the lac repressor, please see David Goodsell's Introduction to the lac repressor in his series Molecule of the Month , and the article in Wikipedia on the lac repressor . (proteopedia.org)
  • An amino acid is the fundamental molecule that serves as the building block for proteins. (genome.gov)
  • Each time an amino acid is added to a growing polypeptide during protein synthesis, a tRNA anticodon pairs with its complementary codon on the mRNA molecule, ensuring that the appropriate amino acid is inserted into the polypeptide. (genome.gov)
Binding4
  • L75F-TrpR is a temperature-sensitive mutant of the tryptophan repressor protein of Escherichia coli in which surface-exposed residue leucine 75 in the DNA binding domain is replaced with phenylalanine. (princeton.edu)
  • retinoblastoma binding protein 2. (atlasgeneticsoncology.org)
  • RS is related to various mutations on the MECP2 gene, which codes for methyl-CpG binding protein-2 (MECP2). (medscape.com)
  • The tox gene is regulated by a corynebacterial iron-binding repressor (DtxR). (medscape.com)
Inhibit1
  • The pLacI plasmid confers chloramphenicol resistance and provides enough lac repressor to inhibit transcription from the T7 lac promoter in the absence of inducer. (sigmaaldrich.com)
Bacterial1
  • The remaining two proteins obtained after HPLC separation were identified as homologues of bacterial repressor-like proteins. (ox.ac.uk)
Histone2
  • The thermostable histone-like protein Sso7c (Sso for Sulfolobus solfataricus) from the archaeon Sulfolobus solfataricus was purified from the supernatant of acid-soluble cell lysates. (ox.ac.uk)
  • A nuclear protein, HDAC-9 belongs to the histone deacetylase family with several isoforms and is inhibited by Trichostatin A (TSA) and suberoylanilide hydroxamic acid. (novusbio.com)
Dimerization1
Binds1
  • When the lac repressor binds lactose, it changes to an inactive conformation that cannot repress the production of these enzymes. (proteopedia.org)
Drosophila3
  • We report that esc protein is highly conserved in housefly (72% identical to Drosophila esc), butterfly (55% identical), and grasshopper (56% identical). (umn.edu)
  • This study used Drosophila melanogaster wing vein and scutellar bristle development to screen Rab proteins predicted to be substrates for ICMT ( Ste14 in flies). (sdbonline.org)
  • Screening more than 200,000 fragments covering the coding sequences of all transcription-related proteins in Drosophila melanogaster, this study identified 195 RDs in known repressors and in proteins not previously associated with repression. (sdbonline.org)
Species1
  • To further investigate the domain organization of esc protein, we have isolated and characterized esc homologs from divergent insect species. (umn.edu)
Homolog1
Recombinant1
  • Engineering lactic acid bacteria (LAB) is of growing importance for food and feed industry as well as for in vivo vaccination or the production of recombinant proteins in food grade organisms. (biomedcentral.com)
Interacts1
  • The extended CAG region changes the structure of atrophin 1 and how the protein interacts with other proteins to control gene function. (medlineplus.gov)
Homeotic1
  • Genetic studies have shown that esc protein is required in early embryos at about the time that other PcG proteins become engaged in homeotic gene repression. (umn.edu)
Functional2
  • Thus, the existence of repressor-like proteins was demonstrated at the protein level in archaea, raising the question of structural and functional consequences of these proteins on the otherwise eukaryotic-like basal transcriptional machinery in archaea. (ox.ac.uk)
  • These findings advance understanding of repressors, their sequences, and the functional impact of sequence-altering mutations and should provide a valuable resource for further studies. (sdbonline.org)
Sequence3
  • The sequence comparisons and modeling suggest that there are seven WD repeats in esc protein which together form a seven-bladed β-propeller. (umn.edu)
  • What percentage of time this DNA sequence spends in a kinked state, in the absence of bound lac repressor protein, is not known, but it may be a significant percentage (see next section below). (proteopedia.org)
  • A protein consists of one or more chains of amino acids (called polypeptides) whose sequence is encoded in a gene. (genome.gov)
Neurons2
  • Although the exact function of this protein is unknown, it appears to play an important role in nerve cells (neurons) in many areas of the brain. (medlineplus.gov)
  • This altered protein accumulates in neurons and interferes with normal cell functions. (medlineplus.gov)
Coli1
  • Novagen Rosetta strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. (sigmaaldrich.com)
Synthesis2
  • In a cell, antisense DNA serves as the template for producing messenger RNA (mRNA), which directs the synthesis of a protein. (genome.gov)
  • The phosphorylated enzyme catalyzes the phosphorylation of the alpha subunit of EUKARYOTIC INITIATION FACTOR-2, leading to the inhibition of protein synthesis. (bvsalud.org)
Complex3
  • NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. (abcam.com)
  • 17-mer used in this work and for the crystal structure of the 2:1 trp repressor/operator complex by Lawson & Carey (1993). (lu.se)
  • 1988) for the d-(ACT)Ád-(AGT) halfsites contacting the repressor in the 1:1 complex. (lu.se)
Function3
  • Site-directed mutagenesis of specific loops, predicted to extend from the propeller surface, identifies conserved parts of esc protein required for function in vivo. (umn.edu)
  • Most of the important insights regarding the specific function of AP-1 proteins have been established through the use of mice with either loss- or gain-of-function manipulations of various members of the bZIP protein family [ 13 ]. (biomedcentral.com)
  • As a result, the protein is altered and cannot function normally, though it is unclear how these changes lead to the specific features of CHEDDA syndrome. (medlineplus.gov)
Expression1
  • Expression host strains for pETBlue and pTriEx vectors are lDE3 lysogens that also carry the compatible pLacI plasmid, encoding the lac repressor. (sigmaaldrich.com)
Structure3
  • Homology modeling using the crystal structure of another WD repeat protein, the G-protein β- subunit, predicts that esc protein adopts a β-propeller structure. (umn.edu)
  • We locate the conserved regions in esc protein with respect to this predicted structure. (umn.edu)
  • Isolation and structure of repressor-like proteins from the archaeon Sulfolobus solfataricus. (ox.ac.uk)
Serine1
  • A dsRNA-activated cAMP-independent protein serine/threonine kinase that is induced by interferon. (bvsalud.org)
Regulator1
  • Transcriptional regulator proteins are closely involved in essential survival strategies in bacteria. (rcsb.org)
Similarities2
  • Despite their many similarities, wild-type and mutant proteins display significant chemical shift differences, one cluster of which is in the B-C turn, too distant to be ascribed solely to ring current effects from Phe75. (princeton.edu)
  • BLAST databases using TBLASTX ( 13 ), to find similarities at protein level. (cdc.gov)
Physical1
  • We suggest that these regions might mediate physical interaction with esc partner proteins. (umn.edu)
Subunit1
  • This protein is the full-length p52 subunit and partial p100 subunit. (abcam.com)
Recognition1
  • Chemistry Department be a major determinant of trp repressor/operator recognition. (lu.se)
Primarily2
  • The esc protein consists primarily of multiple copies of the WD repeat, a motif that has been implicated in protein-protein interaction. (umn.edu)
  • AP-1 proteins are primarily considered to be oncogenic. (biomedcentral.com)
Resistance1
  • Vgsc-interacting proteins are genetically associated with pyrethroid resistance in Aedes aegypti. (cdc.gov)
Repeats1
  • The NUP98/KDM5A fusion protein contains the Phe-Gly (FG) repeats of the N-terminal part of NUP98. (atlasgeneticsoncology.org)
Regulation1
  • who also correctly proposed the general mechanism of regulation by the lac repressor. (proteopedia.org)
MRNA1
  • In MEF cells with wild type Nrf2 and mouse hepa 1c1c7 cells, Cd increased the Nrf2 protein level but not the Nrf2 mRNA level dose-dependently. (cdc.gov)
Specific1
  • While activators have been extensively characterized, repressors are less well studied: the identities and properties of their repressive domains (RDs) are typically unknown and the specific co-repressors (CoRs) they recruit have not been determined. (sdbonline.org)