• Y861 and Y925 (19, 26), also to phosphorylation of FAK binding proteins after that, such as for example paxillin and Cas (27). (cahrr.org)
  • In this study we used phosphorylation of the transcription factor cAMP response element-binding protein as a functional readout to identify cells responding to EGF and FGF-2. (beauty104.com.tw)
  • We previously established SH2 profiling, a phosphoproteomic approach based on membrane binding assays that utilizes purified Src Homology 2 (SH2) domains as a molecular tool to profile the global tyrosine phosphorylation state of cells. (biomedcentral.com)
  • An important consequence of reversible phosphorylation of tyrosine residues on proteins is the creation of binding sites for phosphotyrosine recognizing domains such as Src homology 2 (SH2) and phosphotyrosine binding (PTB) domains [ 1 , 2 ]. (biomedcentral.com)
  • SH2 profiling is a unique proteomic method in which interactions between an array of SH2 domains and protein samples are quantitatively analyzed, thereby defining the functional output of tyrosine phosphorylation. (biomedcentral.com)
  • Kinetics of Src homology 3 domain association with the proline-rich domain of dynamins: specificity, occlusion, and the effects of phosphorylation. (uchicago.edu)
  • Hyaluronan-mediated CD44 interaction with RhoGEF and Rho kinase promotes Grb2-associated binder-1 phosphorylation and phosphatidylinositol 3-kinase signaling leading to cytokine (macrophage-colony stimulating factor) production and breast tumor progression. (uchicago.edu)
  • CD28 enhances TCR-driven tyrosine phosphorylation and interacts with phosphatidylinositol 3-kinase (PI3K) and Grb2, initiating the Akt-mTOR and Ras-MAPK pathways. (medicaltrend.org)
  • This Tks5-reliant increase in intrusive behavior additional depended on Src tyrosine kinase activity as well as the phosphorylation of Tks5 at tyrosine residues 557 and 619. (healthanddietblog.info)
  • In Personal computer-3 cells we proven that Tks5 phosphorylation at these websites was required and adequate for invadopodia-associated matrix degradation and invasion. (healthanddietblog.info)
  • Tks5 has Src phosphorylation sites located between your fourth and third SH3 domains [19]. (healthanddietblog.info)
  • We while others possess proven that Tks5 phosphorylation can be important for podosome development and associated matrix degradation in macrophages and osteoclasts [24 25 In melanoma cells Src-dependent phosphorylation of Tks5 at tyrosine 557 is important for binding to Nck for Nck recruitment to invadopodia and for invadopodia-associated matrix degradation activity [16]. (healthanddietblog.info)
  • These two levels serve degraded by a phosphorylation including at least 6 membranes in a such tail that both leaves the 2' disorder ubiquitination and adducts the 3' heart to the 5' cell. (evakoch.com)
  • Here, we used time-resolved high-throughput proteomic analyses to identify and quantify the phosphorylation events and protein-protein interactions encoding T cell ligand discrimination in antigen-experienced T cells. (nature.com)
  • Although low-affinity ligands induced phosphorylation of the Cd3 chains of the T cell receptor and the interaction of Cd3 with the Zap70 kinase as strongly as high-affinity ligands, they failed to activate Zap70 to the same extent. (nature.com)
  • We defined quantitative signatures of ligand affinity based on protein phosphorylation and protein-protein interaction (PPI) stoichiometry for critical molecular events associated with TCR signaling. (nature.com)
  • While the phosphorylation of tyrosine residues in the immunoreceptor tyrosine-based activation motifs (ITAMs) of Cd3 and the inducible interaction between Cd3 and Zap70 remained largely unaffected, the abundance of activated Zap70 dropped with decreasing peptide affinity. (nature.com)
  • BTK, a TEC-family tyrosine kinase activated by the B-cell antigen receptor, contains a variety of regulatory domains and it is subject to complex regulation by membrane phospholipids, protein ligands, phosphorylation, and dimerization. (elifesciences.org)
  • Measuring protein phosphorylation and expression in glioblastoma cells across 40 signaling pathway nodes in response to different drugs and for different oxygen tensions revealed that SHP2 antagonism has network-level, context-dependent signaling consequences that affect cell phenotypes (e.g., cell death) in unanticipated ways. (aacrjournals.org)
  • Grb2 is best known for its ability to link the epidermal growth factor receptor tyrosine kinase to the activation of Ras and its downstream kinases, ERK1,2. (wikipedia.org)
  • A fly-human cross-species comparison of the phosphoinositide-3-kinase (PI3K) interactome was conducted in a Drosophila S2R+ cell line and several NSCLC and human multiple myeloma cell lines to identify conserved interacting proteins to PI3K, a critical signaling regulator of the AKT pathway. (sdbonline.org)
  • The Src Homology 2 (SH2) domain is a major protein interaction module that is central to tyrosine kinase signaling. (eu.org)
  • The human being FAK (also called PTK2, protein tyrosine kinase 2) gene continues to be mapped to chromosome 8 (15, 16). (cahrr.org)
  • FAK gets the N-terminal, Kinase site as well as the C-terminal domains. (cahrr.org)
  • Kinase site is involved with binding with FIP200 protein. (cahrr.org)
  • This cascade causes following cytoskeletal adjustments and activation of RAS-MAPK (mitogen-activated protein kinase) signaling pathways (26, 28). (cahrr.org)
  • Phosphorylated Y397 FAK can recruit another essential signaling protein, p85 PI3-kinase (phosphoinositide 3-kinase), development factor receptor destined protein Grb 7, phospholipase Cgamma(PLCgamma) and. (cahrr.org)
  • There is a great interest in studying phosphotyrosine dependent protein-protein interactions in tyrosine kinase pathways that play a critical role in many aspects of cellular function. (biomedcentral.com)
  • A signal transducing adaptor protein that links extracellular signals to the MAP KINASE SIGNALING SYSTEM. (uchicago.edu)
  • Mutations in Brutons tyrosine kinase (Btk) within its PH domain cause X-linked agammaglobulinaemia (XLA) in patients. (embl.de)
  • Pleckstrin, the protein where this domain was first detected, is the major substrate of protein kinase C in platelets. (embl.de)
  • BACKGROUND The Src tyrosine kinase substrate and adaptor proteins Tks5 had previously been implicated in the invasive phenotype of normal and transformed cell types via rules of cytoskeletal constructions called podosomes/invadopodia. (healthanddietblog.info)
  • Src is generally upregulated in advanced stage malignancies and activation of Src tyrosine kinase activity transforms cells to a neoplastic phenotype with improved survival development and migration [8-10]. (healthanddietblog.info)
  • Physiologically, the N-terminal domain of p126 binds the Src-homology 3 (SH3) domain of c-Abl, leading to activation of the MAPK/ERK2 kinase. (molcells.org)
  • We overexpress and purify adaptor proteins, Growth factor receptor bound protein 2 (Grb2), Grb2-related adaptor downstream of Shc (Gads), phosphatidylinositol-3 kinase (PI3K), and their SH2 domains, and analyze their molecular interactions with the intracellular regions of CD28, ICOS, and CTLA-4. (kpu-biophyschem.com)
  • This gene is a member of the protein-tyrosine kinase oncogene family. (cancerindex.org)
  • The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. (cancerindex.org)
  • Previous studies have demonstrated that DNA damage-inducible transcript 4 (DDIT4), Trib3 (tribbles pseudo kinase 3), alpha-synuclein (α-syn) are involved in METH-induced dopaminergic neurotoxicity. (cancerindex.org)
  • CryoEM reconstructions support conformational heterogeneity in the PHTH-PRR region wherein the globular PHTH domain adopts a range of states arrayed around the autoinhibited SH3-SH2-kinase core. (elifesciences.org)
  • On the way to activation, disassembly of the SH3-SH2- kinase core opens a new autoinhibitory site on the kinase domain for PHTH domain binding that is ultimately released upon interaction of PHTH with PIP3. (elifesciences.org)
  • Membrane-induced dimerization activates BTK and we present here a crystal structure of an activation loop swapped BTK kinase domain dimer that likely represents the conformational state leading to trans-autophosphorylation. (elifesciences.org)
  • Together, these data provide the first structural elucidation of full-length BTK and allow a deeper understanding of allosteric control over the BTK kinase domain during distinct stages of activation. (elifesciences.org)
  • The TEC and SRC kinases share the SH3-SH2-kinase domain arrangement (the 'Src module') ( Fig. 1a ). (elifesciences.org)
  • Structures of the autoinhibited SRC family kinases were solved in 1997 revealing, for the first time, the compact arrangement of the SH3 and SH2 domains assembled onto the distal side of the catalytic kinase domain [ 4 ]. (elifesciences.org)
  • The 'Src module' is the SH3-SH2-kinase region shred by both families. (elifesciences.org)
  • The compact structure of the SH3-SH2-kinase region of BTK is exacted from the domain swapped dimer structure (PDB: 4XI2) solved by Wang et al. (elifesciences.org)
  • 5 ]. The three domains (SH3, SH2, kinase), the SH2-kinase linker, the activation loop and the active site are labeled. (elifesciences.org)
  • To understand the signal transduction required, we focused primarily on IL-6 induction by measuring mitogen-activated protein kinase activity and analyzing the effects of mutant or dominant negative forms of Vav, Rac1, and c-Jun N-terminal kinase-1 (JNK1). (aai.org)
  • Vav overexpression resulted in the constitutive activation of JNK1 with little or no effect on p38 mitogen-activated protein kinase and ERK2. (aai.org)
  • Growth factor receptor-bound protein 2 also known as Grb2 is an adaptor protein involved in signal transduction/cell communication. (wikipedia.org)
  • The protein encoded by this gene binds receptors such as the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. (wikipedia.org)
  • The mechanism of host cell invasion by SARS-CoV-2 involves the interaction of angiotensin-converting enzyme (ACE2) with receptor-binding domain (RBD) of spike protein (S). The main protease (Mpro/3CLpro) is an attractive drug target due to its vital function in regulation of polyprotein translated from viral RNA. (bvsalud.org)
  • Here, we show that insulin-induced activation of ERKs was enhanced by stable overexpression of growth factor receptor-bound protein 2 (GRB2) but not by overexpression of GRB2 proteins with point mutations in the Src homology 2 and 3 domains. (nih.gov)
  • In contrast to the activated epidermal growth factor receptor that binds the GRB2-Sos complex directly, activation of the insulin receptor results in the interaction of GRB2-Sos with IRS-1 and Shc, thus linking the insulin receptor to Ras signaling pathways. (nih.gov)
  • These data revealed an unexpected direct binding of Corkscrew, the Drosophila ortholog of the non-receptor protein tyrosine phosphatase type II (SHP2) to the Pi3k21B (p60) regulatory subunit of PI3K (p50/p85 human ortholog) but no association with Pi3k92e , the human ortholog of the p110 catalytic subunit. (sdbonline.org)
  • NCK is a major adaptor protein involved in membrane receptor signalling and modulation of actin cytoskeleton dynamics. (eu.org)
  • Receptor dimerization and autophosphorylation attracts proteins containing Src homology 2 (SH2) or phosphotyrosine binding (PTB) domains including adaptor proteins like FRS2 and GRB2. (springer.com)
  • This can either occur by cell surface receptor interactions, as in the case with peptide growth factors, or by interactions with intracellular molecules, as in the case with steroid hormones. (beauty104.com.tw)
  • protein tyrosine phosphatase, non-receptor. (wikigenes.org)
  • Receptor-facilitated antigen presentation requires the recruitment of B cell linker protein to Igalpha. (uchicago.edu)
  • Ser/Thr protein kinases such as the Akt/Rac family, the beta-adrenergic receptor kinases, the mu isoform of PKC and the trypanosomal NrkA family. (embl.de)
  • These motifs are phosphorylated by Src family kinases (SFKs) upon receptor activation and play crucial roles in regulating the immune system. (medicaltrend.org)
  • Programmed cell death protein 1 (PD-1, CD279) is a transmembrane glycoprotein that belongs to the CD28 receptor superfamily. (medicaltrend.org)
  • Src may be the namesake person in a grouped category of non-receptor tyrosine kinases as well as the initial described protooncogene [7]. (healthanddietblog.info)
  • The Grb2-like adaptor protein Mona/Gads associates stably with the T-cell receptor signal transducer SLP-76. (ox.ac.uk)
  • ELAC1 has been in the use and may as define as an RNase Z. In pyrophosphates subfamilies are transcribed from coupling tubules in the function by a two receptor chromatin that appears ultraviolet from protein threatening( reviewed in Popow et al. (evakoch.com)
  • The TSEN placement Typically triggers 5' and 3' to the home', proliferating a downstream' vascular maturation on the 5' T and a 5' receptor nucleus on the 3' mitosis. (evakoch.com)
  • The TEC kinases are the second largest sub-family of non-receptor tyrosine kinases in the human genome after the SRC family [ 1 - 3 ]. (elifesciences.org)
  • In addition to plexins, various other proteins have been implicated either as receptors for semaphorins or as components of the semaphorin receptor complex ( Pasterkamp and Kolodkin, 2003 ). (rupress.org)
  • Insulin-induced activation of extracellular signal-regulated kinases [ERKs, also known as mitogen-activated protein (MAP) kinases] is mediated by Ras. (nih.gov)
  • Tyrosine protein kinases belonging to the Btk/Itk/Tec subfamily. (embl.de)
  • Activated TCR triggers the assembly of a signalosome, with key components including tyrosine kinases like Lck and Zap70, scaffold proteins such as Lat, SLP76, and Themis, and phospholipase Cγ1 (PLC γ1), as well as tyrosine phosphatases and E3 ubiquitin ligases like SHP1 and Cbl, among others. (medicaltrend.org)
  • Though they talk about a number of the same cytoskeletal regulatory equipment (integrins tyrosine kinases Arp2/3 WASp) as additional adhesion constructions they are recognized by marker protein CK-1827452 (Omecamtiv mecarbil) (cortactin dynamin2 Tks5) and metalloproteinases (MT1-MMP) that distinctively support focalized matrix redesigning activity [2-5]. (healthanddietblog.info)
  • Despite the shared 'Src module' of the SRC and TEC families, eighteen years passed before an autoinhibited structure of one of the TEC kinases was resolved by x-ray crystallography. (elifesciences.org)
  • One-half of the domain swapped BTK structure represents the current model for autoinhibited BTK and closely resembles the compact structure of autoinhibited SRC kinases ( Fig. 1b ). (elifesciences.org)
  • Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. (wikipedia.org)
  • The N-terminal SH3 domain binds to proline-rich peptides and can bind to the Ras-guanine exchange factor SOS. (wikipedia.org)
  • The C-terminal SH3 domain binds to peptides conforming to a P-X-I/L/V/-D/N-R-X-X-K-P motif that allows it to specifically bind to proteins such as Gab-1. (wikipedia.org)
  • the preferred GFRα coreceptor for GDNF is GFRα1, although GDNF also weakly binds to GFRα2 and GFRα3 [ 3 ]. (medsci.org)
  • The Tir protein of EPEC binds NCK1/NCK2 SH2 domains through a high affinity pYDEV motif ( Frese,2006 ). (eu.org)
  • Mouse protein citron, a putative rho/rac effector that binds to the GTP-bound forms of rho and rac. (embl.de)
  • Src Homology 2 (SH2) domains are small modular domains found within a great number of proteins involved in different signalling pathways. (eu.org)
  • Among them are the Sprouty proteins which mainly act as inhibitors of growth factor-dependent neuronal and glial signaling pathways. (springer.com)
  • After that we can pay focus on novel therapeutics methods to focus on these pathways and interaction in tumor. (cahrr.org)
  • Through these interactions, PH domains play a role in recruiting proteins to different membranes, thus targeting them to appropriate cellular compartments or enabling them to interact with other components of the signal transduction pathways. (embl.de)
  • What pathways are this gene/protein implicaed in? (cancerindex.org)
  • The FGFs are heparin -binding proteins and interactions with cell-surface associated heparan sulfate proteoglycans has been shown to be essential for FGF signal transduction. (beauty104.com.tw)
  • Structurally, PD-1 consists of extracellular immunoglobulin (Ig)-like variable domains, a transmembrane domain, and a cytoplasmic tail responsible for signal transduction and binding to scaffold molecules. (medicaltrend.org)
  • Tks5 is a substrate of Src and an adaptor protein for proteins and lipids [19]. (healthanddietblog.info)
  • In addition, ST5 enhanced activation of Src and Syk, a Src substrate, in response to RANKL. (molcells.org)
  • The 3' replication has freely activated by RNase Z surface, a mature binding in receptors( reviewed in Maraia and Lamichhane 2011). (evakoch.com)
  • The N-terminal site (1C415 a.a) of FAK protein provides the main autophosphorylation site Con397-tyrosine, that in phosphorylated form becomes a binding site of SH-2 site of Src, resulting in its conformational adjustments and activation (19). (cahrr.org)
  • 2016) . A cross-species study of PI3K protein-protein interactions reveals the direct interaction of P85 and SHP2 . (sdbonline.org)
  • The p85-SHP2 association was validated in human cell lines, and formed a ternary regulatory complex with GRB2-associated-binding protein 2 (human GAB2 but not Drosophila Dos ). (sdbonline.org)
  • Validation experiments with knockdown of GAB2 and Far-Western blots proved the direct interaction of SHP2 with p85, independent of adaptor proteins and transfected FLAG-p85 provided evidence that SHP2 binding on p85 occurred on the SH2 domains. (sdbonline.org)
  • A disruption of the SHP2-p85 complex took place after insulin/IGF1 stimulation or imatinib treatment, suggesting that the direct SHP2-p85 interaction was both independent of AKT activation and positively regulates the ERK signaling pathway. (sdbonline.org)
  • This study identified that the protein most commonly affected in Noonan syndrome, the phosphatase SHP2, known in Drosophila as corkscrew (CSW) , controls life span, triglyceride levels, and metabolism without affecting ERK signaling pathway. (sdbonline.org)
  • Noonan syndrome (NS) and NS with multiple lentigines (NSML) cognitive dysfunction are linked to SH2 domain-containing protein tyrosine phosphatase-2 (SHP2) gain-of-function (GoF) and loss-of-function (LoF), respectively. (sdbonline.org)
  • Oncogenic protein tyrosine phosphatases have long been viewed as drug targets of interest, and recently developed allosteric inhibitors of SH2 domain-containing phosphatase-2 (SHP2) have entered clinical trials. (aacrjournals.org)
  • SH2 domains are phosphotyrosine recognition domains, often mediating transient interactions with target proteins. (eu.org)
  • If the GST-SH2 and EGFR are in close proximity as a result of SH2-phosphotyrosine interactions, the two oligonucleotides are brought within a suitable distance for ligation to occur, allowing for efficient complex amplification via real-time PCR. (biomedcentral.com)
  • The assay detected signal across at least 3 orders of magnitude of lysate input with a linear range spanning 1-2 orders and a low femtomole limit of detection for EGFR phosphotyrosine. (biomedcentral.com)
  • Different families of SH2 domains may have different binding specificity, which is usually determined by a few residues C-terminal with respect to the pY (positions +1 to +4). (eu.org)
  • Inspection of known binders reveals that weak residues are tolerated at pY+3 when pY+1 is strong and vice-versa, but are not tolerated at both positions. (eu.org)
  • The residue at pY+2 does not make direct side chain interactions with the SH2 domain, but aromatic residues are not allowed. (eu.org)
  • Positively charged residues are disfavoured at pY-1 and pY-2 due to the positively charged SH2 domain surface, but are tolerated when pY+1 and pY+3 are strong residues. (eu.org)
  • There are no totally invariant residues within the PH domain. (embl.de)
  • Isoform gamma contains two PH domains, the second one is split into two parts separated by about 400 residues. (embl.de)
  • The central R-x-x-K motif of the peptide forms a 3(10) helix and inserts into a negatively charged double pocket on the SH3C while several other residues complement binding through hydrophobic interactions, creating a short linear SH3C binding epitope of uniquely high affinity. (ox.ac.uk)
  • To further define which neurons express Eph, antibodies were generated to the cytoplasmic portion of the Eph protein. (sdbonline.org)
  • Regions outside of conserved PxxPxR motifs drive the high affinity interaction of GRB2 with SH3 domain ligands. (uchicago.edu)
  • It is thus not surprising that targeted gene disruption of Grb2 in mice is lethal at an early embryonic stage. (wikipedia.org)
  • Directed network wiring identifies a key protein interaction in embryonic stem cell differentiation. (uchicago.edu)
  • c-Met and RON have essential functional roles in embryonic development and organogenesis [ 3 , 4 ] and are over expressed and/or aberrantly activated in various cancer types suggesting their potential importance as therapeutic targets [ 5 - 10 ]. (oncotarget.com)
  • The communication in subunit: enabling lymphoid GT-domains preventing membrane as a subunits addition: A ATM identified to the Faculty of Graduate Studies and Research in isolated pore of the heterotrimers for the use of Master of Nursing. (evakoch.com)
  • They are able to bind specific motifs containing a phosphorylated tyrosine residue, propagating the signal downstream by promoting protein-protein interactions and/or modifying enzymatic activities. (eu.org)
  • GRB2 overexpression led to increased formation of a complex between the guanine nucleotide-releasing factor Sos (the product of the mammalian homolog of son of sevenless gene) and GRB2. (nih.gov)
  • As the main component, extracellular structure contains some cysteine-rich repeats domains marked as D1-D2-D3 in GFRα1-3, and D2-D3 in GFRα4 ( Figure 1 a ). (medsci.org)
  • Tumor cell motility and extracellular matrix SAT1 degradation are backed by cytoskeletal constructions known as invadopodia [2 3 Invadopodia as well as the related podosomes of regular cell types are actin-based cell surface area protrusions that enable adherence to and degradation of extracellular matrix proteins. (healthanddietblog.info)
  • Non-phosphorylated peptides do not bind to the SH2 domains. (eu.org)
  • CagA can specifically bind to the SH2 domain of Src homology 2 (SH2)-containing protein tyrosine phosphatase (SHP-2), which induces spatial configuration change of SHP-2 and activates it [ 40 ]. (biomedcentral.com)
  • The domain family possesses multiple functions including the abilities to bind inositol phosphates, and various proteins. (embl.de)
  • Several different binding motifs are known, for example: pYEEI (Src-family SH2 domains), pY [IV]. (eu.org)
  • The C-terminal half of the SH2 domain exhibits greater structural variability and provides a platform for accommodating different kinds of SH2-binding motifs. (eu.org)
  • Noonan syndrome and related disorders are caused by mutations in genes encoding for proteins of the RAS-ERK1/2 signaling pathway, which affect development by enhanced ERK1/2 activity. (sdbonline.org)
  • Regulators of small G-proteins like guanine nucleotide releasing factor GNRP (Ras-GRF) (which contains 2 PH domains), guanine nucleotide exchange proteins like vav, dbl, SoS and Saccharomyces cerevisiae CDC24, GTPase activating proteins like rasGAP and BEM2/IPL2, and the human break point cluster protein bcr. (embl.de)
  • Rho GTPase activating protein 24 [Source. (gsea-msigdb.org)
  • Val and Pro confer high-affinity binding at pY+3 - Ala and Ile are tolerated but confer weaker binding. (eu.org)
  • The binding affinity of an SH2 domain to a pTyr containing ligand is moderate, with the typical affinity range between 0.1 µМ to 10 µМ for equilibrium dissociation constant values (Kd) ( Kaneko,2012 ). (eu.org)
  • PD-1 is activated through its interaction with PD-L1 (B7-H1, CD274) and exhibits higher affinity for PD-L2 (B7-DC, CD273). (medicaltrend.org)
  • Structural basis for SH3 domain-mediated high-affinity binding between Mona/Gads and SLP-76. (ox.ac.uk)
  • SH3 domains are protein recognition modules within many adaptors and enzymes. (ox.ac.uk)
  • The semaphorin superfamily are composed of three protein families, the semaphorins, plexins and the c-Met family [ 2 ]. (oncotarget.com)
  • Plexins are widely expressed transmembrane proteins that mediate the effects of semaphorins. (rupress.org)
  • Their effects are mediated by plexins, a group of transmembrane proteins. (rupress.org)
  • The interaction between SH2 domains and their substrates is however dependent also on cooperative contacts of other surface regions. (eu.org)
  • Src activation also frequently promotes podosome/invadopod development [4 11 That is backed by the current presence of tyrosine phosphorylated proteins at these constructions many of that are Src substrates [14-18]. (healthanddietblog.info)
  • Over 120 SH2 domains are predicted in the human genome ( Liu,2011 ). (eu.org)
  • With more than 500 SH3 domains in the human genome, binding selectivity is a key issue in understanding the molecular basis of SH3 domain interactions. (ox.ac.uk)
  • Precisely how the BTK N-terminal domains (the Pleckstrin homology/Tec homology (PHTH) domain and proline-rich regions (PRR) contain linker) contribute to BTK regulation remains unclear. (elifesciences.org)
  • Mutations identified in many SH2 domain-containing proteins as well as the SH2 domain itself are associated with human diseases ranging from cancers, diabetes, to immunodeficiencies. (eu.org)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • Interestingly, the SH3C displays ion-dependent dimerization in the crystal and in solution, suggesting a novel mechanism for the regulation of SH3 domain functions. (ox.ac.uk)
  • Pleckstrin homology domain. (embl.de)
  • Pleckstrin homology (PH) domains are small modular domains that occur in a large variety of proteins. (embl.de)
  • Pleckstrin is one of the rare proteins to contains two PH domains. (embl.de)
  • The cytotoxin-associated gene A ( cagA ) is one of the most important virulence genes of H. pylori , which is located at the end of cag pathogenicity island ( cag PAI) and encodes the 120-145 kDa CagA protein [ 7 ]. (biomedcentral.com)
  • This presents a starting point for studies into the underlying mechanisms of AD including interactions with other AD-associated genes, like Rho1, Ankyrin, Tau and APP with the potential to identify new targets for treatment. (sdbonline.org)
  • ITIMs negatively modulate cell activation by recruiting phosphatases such as protein tyrosine phosphatases 1 and 2 (SHP-1 and SHP-2), as well as inositol 5′-phosphatases 1 and 2 (SHIP-1 and SHIP-2). (medicaltrend.org)
  • Emerging evidence provided insight into the role of signal transducer and activator of transcription 3 (STAT3) in energy metabolism. (biomedcentral.com)
  • ALF transcription elongation factor 3 [S. (gsea-msigdb.org)
  • Cytoskeletal proteins such as dynamin (see IPR001401 ), Caenorhabditis elegans kinesin-like protein unc-104 (see IPR001752 ), spectrin beta-chain, syntrophin (2 PH domains) and S. cerevisiae nuclear migration protein NUM1. (embl.de)
  • Grb2 has two closely related proteins with similar domain organizations, Gads and Grap. (wikipedia.org)
  • The crystal structure of a complex between the C-terminal SH3 domain (SH3C) of Mona/Gads and a SLP-76 peptide has now been solved to 1.7 A. The peptide lacks the canonical SH3 domain binding motif P-x-x-P and does not form a frequently observed poly-proline type II helix. (ox.ac.uk)
  • This interaction was modeled on a demonstrated interaction between human Dyn2 and Gads from an unspecified species. (uth.edu)
  • The results can be applied in protein engineering to increase protein stability and function, and further, for rational design of small molecules to inhibit molecular interactions. (kpu-biophyschem.com)
  • Grb2 is widely expressed and is essential for multiple cellular functions. (wikipedia.org)
  • Microtubule dynamics is modulated by many cellular factors including stathmin family proteins. (bvsalud.org)
  • To achieve our projects, we purify proteins, and analyze the physical properties and molecular interactions using biophysical methods such as circular dichroism (CD), isothermal titration calorimetry (ITC), and surface plasmon resonance (SPR) biosensor. (kpu-biophyschem.com)