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  • inhibition
  • To understand the molecular events involved in inhibition of FPPS by N-BPs, we used protein crystallography, enzyme kinetics, and isothermal titration calorimetry. (ox.ac.uk)
  • mTOR
  • All these changes are promoted by the metabolic checkpoint kinase mechanistic target of rapamycin (mTOR) that controls protein translation, cell growth, and metabolism ( 3 , 4 ). (frontiersin.org)
  • transcription
  • Glycolysis-driven lipogenesis is induced by Akt signaling and depends on sterol regulatory element-binding protein (SREBP) transcription factors. (frontiersin.org)
  • inhibits
  • Ajoene, a garlic compound, inhibits protein prenylation and arterial smooth muscle cell proliferation. (semanticscholar.org)
  • Recent studies have demonstrated that TSC2 displays GAP (GTPase-activating protein) activity specifically towards the small G protein Rheb and inhibits its ability to stimulate the mTOR signaling pathway. (asm.org)
  • mTOR, a protein kinase, is a central controller of cell growth and stimulates phosphorylation of two key translation regulators, ribosomal S6 kinase (S6K) and eukaryote initiation factor 4E binding protein (4EBP1) ( 5 , 6 , 14 , 24 , 44 ), whereas TSC1/2 inhibits the phosphorylation of both S6K and 4EBP1 ( 38 ). (asm.org)
  • target proteins
  • We describe a new technique for detecting prenyl anchors in N-terminally glutathione S-transferase (GST)-labeled constructs of target proteins expressed in vitro in rabbit reticulocyte lysate and incubated with 3 H-labeled anchor precursors. (biology-online.org)
  • Savings in time for experimentation and the higher sensitivity for detecting 3 H-labeled lipid anchors recommend the TLC-scanning method with purified GST- (or HA-) tagged target proteins as the method of choice for analyzing their prenylation capabilities in vitro and in vivo and, possibly, also for studying the myristoyl and palmitoyl posttranslational modifications. (biology-online.org)
  • pathway
  • Prenylation is thought to be the mechanism by which statin treatment makes fruit flies live significantly longer - interestingly, it appears that the effect works through improvements in their small fruit fly hearts, which might well be a mechanism by which statins improve cardiovascular mortality in humans completely outside the LDL pathway. (sciencemag.org)
  • motifs
  • We showed that CRACR2A (Ca 2+ release-activated Ca 2+ channel regulator 2A) encodes a lymphocyte-specific large Rab GTPase that contains multiple functional domains, including EF-hand motifs, a proline-rich domain (PRD), and a Rab GTPase domain with an unconventional prenylation site. (sciencemag.org)
  • lamin
  • He teamed up with Mike Gelb in a very fruitful collaboration, and together they identified a protein (lamin B) that was covalently modified by a farnesyl moiety. (asbmb.org)
  • kinase
  • The present data show that the splicing factor SF3b1 is a substrate of the protein kinase DYRK1A and suggest that DYRK1A may be involved in the regulation of pre mRNA-splicing. (biology-online.org)
  • CaaX
  • Wild-type and CAAX-containing variants of GFP- YPT1 ,- SEC4 ,- YPT6 ,- YPT7 , and - VPS21 were cloned in expression plasmids at wild-type protein levels. (nih.gov)
  • The genes encoding CaaX protein prenyltransferases are considerably longer than those encoding non-CaaX subunits, as a result of longer introns. (nih.gov)
  • b) genes encoding non-CaaX protein prenyltransferases are much shorter. (nih.gov)
  • evolution
  • A specific feature of the angiosperm Rab escort protein (REP) and evolution of the REP/GDI superfamily. (semanticscholar.org)
  • Multiple domain insertions and losses in the evolution of the Rab prenylation complex. (nih.gov)
  • We profiled the domain architecture of the components of the Rab prenylation complex in evolution. (nih.gov)
  • We found different domain insertions in different taxa, in alpha-subunits of RGGTase and REP. Our results suggest that there were multiple insertions, expansions and contractions in the evolution of this prenylation complex. (nih.gov)
  • cascades
  • However, in most cases, it remains unknown at which point in the various signaling cascades farnesylated signaling proteins have their effects in plants. (plantcell.org)
  • tumor
  • Messing around with prenylation can have a number of effects, including tumor progression and modulation of the immune system . (sciencemag.org)
  • The researcher Zafira Castaño has discovered that the loss of a protein in the early phases of lung cancer favors tumor growth. (biology-online.org)
  • A decade of research has led to improved understanding of the tumor suppressor functions of hamartin and tuberin, the protein products of TSC1 and TSC2 , respectively. (aacrjournals.org)
  • ciliary
  • RPGR (retinitis pigmentosa GTPase regulator), a major cause of retinitis pigmentosa (RP) worldwide, is a ciliary protein involved in regulating protein trafficking and composition of cilia. (arvojournals.org)
  • Our results suggest that prenylation modulates the trafficking of a regulator of ciliary composition and indicate the involvement of mistrafficking of RPGR as a potential mechanism of associated retinal ciliopathy. (arvojournals.org)
  • Rpgr ex1-19 and Rpgr ORF15 expression during retinal development were compared using immunoblot analysis and immunohistochemistry, and ciliary affinity in adult photoreceptors was assessed by protein fractionation. (arvojournals.org)
  • TSC2
  • TSC1 and TSC2 form a complex (TSC1/2) in intact cells, and the interaction between TSC1 and TSC2 appears to be important for the stability of both proteins and for their physiological functions ( 27 ). (asm.org)
  • TSC2 contains a region of limited homology to the catalytic domain of the Rap1-GTPase-activating protein (GAP) ( 16 ). (asm.org)
  • biology
  • COMMENT This work, from the laboratories of Ken Kosik and Songi Han, provides important new insight into the biology of tau protein. (alzforum.org)
  • interact
  • We have identified a factor, Yip1p that specifically binds the di-geranylgeranylated Rab and does not interact with mono-prenylated Rab proteins. (nih.gov)
  • cells
  • Moreover, the suppression of ABA-induced calcium increases in guard cells by the dominant protein phosphatase 2C mutant abi2-1 was rescued partially in era1-2 abi2-1 double mutant guard cells, further reinforcing the notion that ERA1 functions upstream of cytosolic calcium and indicating the genetic interaction of these two mutations upstream of ABA-induced calcium increases. (plantcell.org)
  • Known
  • The structural motif discovery method presented herein is general and can be applied to any set of proteins with known 3D structures. (biology-online.org)