• Intracellular proteoglycan core protein precursor was purified from cell lysates by immunoprecipitation with polyclonal antibodies against the hyaluronic acid-binding region, followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. (lu.se)
  • These results indicate, in agreement with our previous kinetic data, that the major part of the xylosyl transfer to the chondrosarcoma proteoglycan core protein precursor must occur late in the processing sequence, i.e. after about 85% of its intracellular lifetime and no more than 7 min before the addition of the rest of the chondroitin sulfate chain. (lu.se)
  • Rat chondrosarcoma chondrocytes were labeled with [ 3 H]serine or [ 3 H]mannose as a precursor. (lu.se)
  • The core precursor was eluted from the gels and treated with alkaline borohydride in order to convert serine residues substituted with xylose or N-acetylgalactosamine to alanine (or with alkaline sulfite to convert them to cysteic acid). (lu.se)
  • These data provide further support that the core precursor resides mainly in the pre-Golgi compartment and that xylosylation occurs mainly in a Golgi compartment. (lu.se)
  • This indicates the presence of high mannose, N-linked oligosaccharides on the core protein precursor which are converted to the complex forms on the completed proteoglycan. (lu.se)
  • Levels of glial fibrillary acidic protein were also increased by PGE 2 treatment, suggesting that these cultured astrocytes resemble reactive astrocytes found in vivo . (jneurosci.org)
  • Reactive astrocytes with increased glial fibrillary acidic protein (GFAP) levels are associated with aging. (jneurosci.org)
  • This inhibition of ER-to-Golgi traffic was not observed upon expression of the processed proteins NS4A and NS4B, singly or in combination. (asm.org)
  • Intracellular proteoglycan core protein precursor was purified from cell lysates by immunoprecipitation with polyclonal antibodies against the hyaluronic acid-binding region, followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. (lu.se)
  • These data provide further support that the core precursor resides mainly in the pre-Golgi compartment and that xylosylation occurs mainly in a Golgi compartment. (lu.se)
  • To determine whether the expression of HCV nonstructural proteins alters ER function, we tested the effect of expression of NS2/3/4A, NS4A, NS4B, NS4A/B, NS4B/5A, NS5A, and NS5B from genotype 1b HCV on anterograde traffic from the ER to the Golgi apparatus. (asm.org)
  • Only the nominal precursor protein NS4A/B affected the rate of ER-to-Golgi traffic, slowing the rate of Golgi-specific modification of the vesicular stomatitis virus G protein expressed by transfection by approximately threefold. (asm.org)
  • Gamma-carboxylated proteins are moved by anterograde transport from the endoplasmic reticulum to the Golgi apparatus (Kirchhausen 2000). (reactome.org)
  • Professor Kenneth Kosik describes senile plaques, an extracellular collection of a-beta protein. (dnalc.org)
  • In particular, similarities in post-translational processing have invited comparisons to the signaling role of the surface receptor protein Notch. (wikipedia.org)
  • In this import process, many cellular proteins such as a cytosolic factor and receptor for the mitochondrial protein precursors are required as import machinery. (nii.ac.jp)
  • Publications] Hideyu Ono: 'Purification of the putative import-receptor for the precursor of the mitochondrial protein' Journal of Biochemistry. (nii.ac.jp)
  • Publications] Hideyu Ono and Syozo Tuboi: 'Purification of the putative import-receptor for the precursor of the mitochondrial protein' J. Biochem.107. (nii.ac.jp)
  • The function of APP may be: (1) G protein-coupled receptor-like role to participate in transmembrane signal transduction process. (biology-online.org)
  • The stimulation by PGE 2 of APP synthesis was mimicked or blocked by activators or inhibitors, respectively, of protein kinase A. Actinomycin D or cycloheximide also inhibited the increase in APP holoprotein stimulated by PGE 2 . (jneurosci.org)
  • However, no age-dependent increase in brain ferritin iron saturation was observed in APP-KO mice despite similar protein expression levels potentially explaining the vulnerability of APP-KO mice to parkinsonism and traumatic brain sequelae. (springer.com)
  • The purified fraction contained two major proteins with molecular masses of 29 and 52 kDa. (nii.ac.jp)
  • Our data support a growing consensus that FMRP binds to guanine-rich domains of some dendritic mRNAs, suppressing their translation and suggest that AD (neurodegenerative disorder) and FXS (neurodevelopmental disorder) may share a common molecular pathway leading to the overproduction of APP and its protein-cleaving derivatives. (pubmedcentralcanada.ca)
  • Pro-sequences are areas in the protein that are essential for its correct folding, usually in the transition of a protein from an inactive to an active state. (wikipedia.org)
  • We may be able to kick-start healing after a traumatic brain injury by boosting levels of a protein made naturally within the brain, according to new research conducted at the University of Adelaide. (medicalxpress.com)
  • Alternatively, presenilin/nicastrin-mediated γ-secretase processing of C99 releases the amyloid beta proteins, amyloid-beta 40 (Aβ40) and amyloid-beta 42 (Aβ42), major components of amyloid plaques, and the cytotoxic C-terminal fragments, gamma-CTF(50), gamma-CTF(57) and gamma-CTF(59). (biolegend.com)
  • Some protein precursors are secreted from the cell. (wikipedia.org)
  • It is involved in cell mobility and transcription regulation through protein-protein interactions. (biolegend.com)
  • Professor Kenneth Kosik discusses neurofibrillary tangles, which form inside a cell and are made up of a protein called tau. (dnalc.org)
  • For example, patients with hereditary cerebral hemorrhage with amyloidosis-Dutch type, an autosomal-dominant severe form of CAA caused by a point mutation in amyloid precursor protein (APP), do not seem to develop significantly more amyloid plaques or neurofibrillary tangles than the normal elderly ( 2 ). (pnas.org)
  • p>This subsection of the 'Function' section indicates at which position the protein binds a given metal ion. (uniprot.org)
  • And it was comfirmed by further analysis that these three proteins function as translocation machinery for the mitochondrial protein precursors. (nii.ac.jp)