Protein overexpression. Medical search. Frequent questions

In this study, we showed that the human RFC (hRFC) gene can serve as a novel selectable marker for the overproduction of recombinant proteins. (aspetjournals.org)
These results establish a novel RFC-based metabolic selection system for the efficient overexpression of recombinant proteins. (aspetjournals.org)
Most Escherichia coli overexpression vectors used for recombinant protein production (RPP) depend on organic inducers, for example, sugars or simple conjugates. (aston.ac.uk)
This includes the transcription of the recombinant DNA to messenger RNA (mRNA), the translation of mRNA into polypeptide chains, which are ultimately folded into functional proteins and may be targeted to specific subcellular or extracellular locations. (wikipedia.org)
The effects of the overexpression of recombinant uncoupling protein 2 on proliferation, migration and plasminogen activator inhibitor 1 expression in human vascular smooth muscle cells. (cims-ops.cz)

FKRP Protein LS-G110010 is an Over-Expression Lysate FKRP with Myc-DDK (Flag) tag(s). (lsbio.com)
Quality control test: Transient overexpression cell lysate was tested with Anti-STX2 antibody by Western Blots. (novusbio.com)
Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). (novusbio.com)
Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. (novusbio.com)

Here, we identify the molecular chaperones heat shock proteins (Hsp) 90α and β as target genes of both pathways. (ashpublications.org)
His work has a strong focus on breast cancer, including risk factors, mutation patterns, overexpression of heat shock proteins, and triple-negative breast cancer. (who.int)

Artemin, a neuronal survival factor in the glial cell line-derived neurotrophic factor family, binds the glycosylphosphatidylinositol-anchored protein GFRα3 and the receptor tyrosine kinase Ret. (jneurosci.org)
Topology prediction shows that there are two N-glycosylation sites, three protein kinase C phosphorylation sites, two casein kinase II phosphorylation sites, four N-myristoylation sites, two amidation sites, and one ribosomal protein S11 signature in the RPS14 protein of the giant panda. (geneticsmr.com)
Overexpression of mitogen-activated protein kinase kinase (MAPKK) and its mutants in NIH 3T3 cells. (tau.ac.il)
Mitogen-activated protein kinase kinase (MAPKK) is a dual specificity protein kinase that exhibits a high degree of specificity toward its downstream target, mitogen-activated protein kinase (MAPK). (tau.ac.il)
Overexpression of tau kinase cdk5 activator p25 resulted in gliosis. (europa.eu)

To simplify this and to cut the cost of RPP, we have developed vectors controlled by the Escherichia coli nitrate-responsive NarL transcription activator protein, which use nitrate, a cheap, stable, and abundant inorganic ion, to induce high-level controlled RPP. (aston.ac.uk)
We recommend aliquoting your vectors into low protein binding tubes upon receipt. (vectorbiolabs.com)

Over-expression is typically done with overexpression of protein A from a plasmid containing the coding sequence, driven by a strong promoter that causes high levels of protein synthesis. (stackexchange.com)

In a newly published paper in the journal Breast Cancer Research, scientists at The Graduate Center of The City University of New York and Hunter College are the first to report that the MDMX protein promotes metastasis of triple-negative breast cancer. (cuny.edu)
Caption: The MDMX and MDM2 proteins promote metastasis in triple-negative breast cancer, while the MDM2 protein promotes tumor growth in estrogen receptor a-positive breast cancer. (cuny.edu)
Our results indicate that mdm2/p53 co-overexpression, nuclear grade, and preoperative presence of distant metastasis are independent predictors for poor survival. (aacrjournals.org)
Interestingly, while KLF6-SV1 overexpression increased metastasis, it did not affect localized tumor growth. (jci.org)

Overexpression of Rice Auxilin-Like Protein, XB21, Induces Necrotic Lesions, up-Regulates Endocytosis-Related Genes, and Confers Enhanced Resistance to Xanthomonas oryzae pv. (escholarship.org)
RNA sequencing reveals that XB21 overexpression results in the differential expression of 8735 genes (4939 genes up- and 3846 genes down-regulated) (≥2-folds, FDR ≤0.01). (escholarship.org)
The up-regulation of genes controlling 'vesicle-mediated transport' in XB21 overexpression lines is consistent with a functional role for XB21 as an auxilin. (escholarship.org)
To address these concerns, expressions systems using multiple eukaryotic cells were developed for applications requiring the proteins be conformed as in, or closer to eukaryotic organisms: cells of plants (i.e. tobacco), of insects or mammalians (i.e. bovines) are transfected with genes and cultured in suspension and even as tissues or whole organisms, to produce fully folded proteins. (wikipedia.org)
6q24-related transient neonatal diabetes mellitus is caused by the overactivity (overexpression) of certain genes in a region of the long (q) arm of chromosome 6 called 6q24. (medlineplus.gov)
There are three ways that overexpression of imprinted genes in the 6q24 region can occur. (medlineplus.gov)
The third mechanism by which overexpression of genes in the 6q24 region can occur is by impaired silencing of the maternal copy of the genes (maternal hypomethylation). (medlineplus.gov)
Cellular DNA-binding proteins encoded by the rel gene (GENES, REL). (bvsalud.org)
135 antibodies against 65 mainly immunoregulatory proteins, we of a multitude of different genes (8, 9). (lu.se)

NFAT2, as an important transcriptional promoter, regulates expression of TNF-α, myc proto-oncogene protein (c-myc), cyclooxygenase-2 (COX-2), Fas ligand (FasL) and also generates crosstalks with ERK/MAPK pathway and AKT/GSK3b signaling, which achieves its control of the cell fate [7-12]. (researchsquare.com)
Taken together, these findings demonstrate for the first time that the cell transformation and tumorigenesis induced by CdCl(2) are due, at least in part, to the overexpression of TIF3, a novel cadmium -responsive proto-oncogene. (cdc.gov)

This resulted in a higher Cytochrome b 6 f content in mesophyll and bundle sheath cells without marked changes in the abundances of other photosynthetic proteins. (essex.ac.uk)
The lectures address the three different main types of membrane proteins and associated cellular processes: transport and transporters, signal transduction and receptors, bioenergetics and photosynthetic and respiratory proteins. (lu.se)

HER2 overexpression or HER2 gene amplification as determined by an accurate and validated assay (see sections 4.4 and 5.1). (who.int)
Assessment of HER2 Protein Overexpression and Gene Amplification in Renal Collecting Duct Carcinoma: Therapeutic Implication. (cdc.gov)

For example, a DNA sequence for a protein of interest could be cloned or subcloned into a high copy-number plasmid containing the lac (often LacUV5) promoter, which is then transformed into the bacterium E. coli. (wikipedia.org)

The overexpression of mdm2 and p53 proteins was investigated on paraffin-embedded material by using monoclonal antibodies. (aacrjournals.org)

Syntaxin 2, also known as epimorphin, is a 35 kDa type II integral membrane that belongs to the t SNARE family, a group of proteins involved in protein transport. (novusbio.com)
The ATP-binding cassette (ABC) of active transporters comprises a group of proteins that which facilitate efflux of anticancer drugs from cancer cells. (lu.se)

p53 protein is involved in cell cycle control but can be inactivated by binding to mdm2 or viral proteins. (aacrjournals.org)

Furthermore, the changes in the expression of these three TJ proteins were consistent with the changes in the BBB permeability, indicating that weakening intercellular junctions leads to BBB opening. (spandidos-publications.com)

In this study, we used stable overexpression of MAPKK and its mutants in NIH 3T3 cells to study effects on downstream components of the MAPK signaling cascade and to correlate them to physiological responses. (tau.ac.il)

The present study confirms the upregulation of TERT in primary glioblastomas while all GABP proteins rise with the malignancy of the gliomas. (oncotarget.com)

These same constructs were neutral within the context of Slpr-dependent developmental signaling, reflecting differential subcellular protein localization and by inference, point of activation. (gardos-channel.com)

The predicted protein encoded by TIF3 cDNA exhibited 99% similarity to human eukaryotic initiation factor 3 p36 protein. (cdc.gov)
A M(r) 36,000 protein was detected in cells transfected with an expression vector containing TIF3 cDNA. (cdc.gov)
Transfection of NIH3T3 cells with an expression vector containing TIF3 cDNA resulted in overexpression of the encoded protein, and this was associated with cell transformation, as evidenced by the appearance of transformed foci exhibiting anchorage-independent growth on soft agar and tumorigenic potential in nude mice. (cdc.gov)

Toward this end, a hemagglutinin (HA) epitope tagged hRFC (hRFC-HA) was introduced into a bicistronic vector (pIRES2-EGFP), upstream of an enhanced green fluorescent protein (EGFP) reporter gene. (aspetjournals.org)
We show that target proteins, such as green fluorescent protein, human growth hormone, and single-chain variable region antibody fragments can be expressed to high levels using our promoter systems. (aston.ac.uk)
Real-time quantitative PCR (RT-qPCR) analysis and sfGFP (superfolder green fluorescent protein) analysis reveal that AmtR directly and negatively regulates the transcription and expression of crn A, cre T, csh A, and hyu B. A growth test shows that C. glutamicum ATCC 14067 can use creatine or creatinine as a sole nitrogen source. (frontiersin.org)

Using an integrated platform of metabolic labeling, high-resolution mass spectrometry and computational analysis, we report here a comprehensive dataset of the in vivo half-life of 3,228 and the expression of 8,064 cardiac proteins, quantified under healthy and hypertrophic conditions across six mouse genetic strains commonly employed in biomedical research. (nature.com)
The dataset contains over 1.92 million data points in protein isotope labeling kinetics, culminating as the in vivo turnover rates of 3,228 cardiac proteins and the expression levels of 8,064 proteins. (nature.com)
Axonal transport rates in vivo are unaffected by tau deletion or overexpression in mice. (alzforum.org)
Further, we studied the effect of HSP70-2 protein ablation on signaling cascades involved in apoptosis, cell cycle and Epithelial-Mesenchymal-Transition both in culture as well as in-vivo human breast xenograft mouse model. (biomedcentral.com)
PM 2.5 decreased the protein level of SIRT1 but not the mRNA expression in vitro and in vivo experiments. (medsci.org)

Now, new work shows that SIRT6 overexpression leads to a reduction in frailty and lifespan extension in both male and female B6 mice. (genengnews.com)
Ultrastructural analysis revealed that overexpression of CD97 strengthened lateral cell-cell contacts between enterocytes, which, in contrast, were weakened in CD97 knockout (Ko) mice. (nih.gov)
We report here a large dataset of protein turnover dynamics in the heart of six common genetic strains of mice, acquired under both normal and hypertrophic conditions. (nature.com)
Asante EA , Linehan J , Desbruslais M , Joiner S , Gowland I , Wood A , BSE prions propagate as either variant CJD-like or sporadic CJD-like prion strains in transgenic mice expressing human prion protein. (cdc.gov)
Overexpression of the Parkinson-related protein, α-synuclein, leads to blood-brain barrier leakage and pericyte activation in mice. (lu.se)

Protein stability is a major regulatory principle of protein function and cellular homeostasis. (nature.com)
Proteins with quantified dynamics belong to over 10 major cellular compartments and over 200 known pathways. (nature.com)
The p53 interacting partner protein NUMB (homology of numb protein) preserves this intrinsic cellular asymmetry by preventing ubiquitin-mediated proteolysis of p53 catalyzed by the MDM2 E3 ubiquitin ligase 11 . (nature.com)
Dr. Maity obtained his Ph.D. in Cellular and Molecular Biology from the Life Science and Biotechnology department at Jadavpur University, India in 2017, where he studied the DNA damage repair protein WRN and autophagy. (nih.gov)

GA-binding proteins selectively bind at these positions, respectively, causing an activation of the promoter and overexpression of TERT. (oncotarget.com)
The p53 protein regulates the mdm2 gene at the level of transcription by an intronic promoter. (aacrjournals.org)
The techniques for overexpression in E. coli are well developed and work by increasing the number of copies of the gene or increasing the binding strength of the promoter region so assisting transcription. (wikipedia.org)
Addition of IPTG (a lactose analog) activates the lac promoter and causes the bacteria to express the protein of interest. (wikipedia.org)

When it was fused with the N-terminally His-tagged protein, it gave rise to accumulation of an expected 22-kDa polypeptide, in good agreement with the predicted molecular weight. (geneticsmr.com)
Hilton DA , Ghani AC , Conyers L , Edwards P , McCardle L , Ritchie D , Prevalence of lymphoreticular prion protein accumulation in UK tissue samples. (cdc.gov)

CD97 overexpression upregulated membrane-bound but not cytoplasmic or nuclear beta-catenin and reduced phospho-beta-catenin, labeled for degradation. (nih.gov)
The TP53 gene is located on the short arm of chromosome 17 (17p13.1) that encodes the nuclear protein p53, whereas the mdm 2 gene maps to the long arm of chromosome 12(12q13-14) and encodes mdm2 protein (1) . (aacrjournals.org)
Nonetheless, bacterial expression has the advantage of easily producing large amounts of protein, which is required for X-ray crystallography or nuclear magnetic resonance experiments for structure determination. (wikipedia.org)
His graduate thesis focused on the nuclear protein Werner (WRN) and autophagy. (nih.gov)

The NFAT2 overexpression suppressed the cell viability, invasion and migration capabilities, and promoted apoptosis of HepG2 cells. (researchsquare.com)
In this study, we constructed NFAT2 overexpressed cells (HepG2/NFAT2) based on HepG2 and investigated the effect of NFAT2 overexpression on HepG2 cells' viability, apoptosis, proliferation, invasion and migration. (researchsquare.com)

The adhesion G-protein-coupled receptor CD97 is present in normal colonic enterocytes but overexpressed in colorectal carcinoma. (nih.gov)
Protein arginine methyltransferase 1 may be involved in pregnane x receptor-activated overexpression of multidrug resistance 1 gene during acquired multidrug. (oncotarget.com)
Pregnane x receptor (PXR) - activated overexpression of the multidrug resistance 1 (MDR1) gene is an important way for tumor cells to acquire drug resistance. (oncotarget.com)
Receptor for ATP and UTP coupled to G-proteins that activate a phosphatidylinositol-calcium second messenger system. (vectorbiolabs.com)
We located that overexpression in the wild-type TSH Receptor Synonyms kinases stimulated JNK signaling in alternate contexts, so cells had been capable of responding to each MAP3Ks, but with distinct outcomes. (gardos-channel.com)

The experimental details depend a lot on what cell type and protein you are studying, of course. (stackexchange.com)
How would one go about identifying unknown cell cycle checkpoint proteins (i.e., cyclins)? (stackexchange.com)
When biopharmaceuticals are produced with one of these systems, process-related impurities termed host cell proteins also arrive in the final product in trace amounts. (wikipedia.org)
This point is driven home by the data reported here because the results on tau overexpression do not support the cell culture reports by Stamer et al. (alzforum.org)
The purpose of this study was to investigate the effect of P53 protein over-expression and some clinicopathological factors on the esophageal squamous cell carcinoma (SCC) response to neoadjuvant chemoradiotherapy. (iranpath.org)
Cell-fate-determinant molecule NUMB-interacting protein (TBC1D15) is overexpressed and contributes to p53 degradation in TICs. (nature.com)
Targeted overexpression or knockdown of PIP5K1α were introduced into BC cell lines. (lu.se)
We focused on the gene amplification and protein expression of ABCC3 (also known as MRP3) in breast cancer cell lines and clinical tumor samples. (lu.se)

Lectures dealing with methods for theoretical modelling of membrane protein structure, fusion protein techniques, X-ray crystallography, heterologous expression, solubilisation and purification of membrane proteins are also included in the course. (lu.se)
In silico exercise addressing potential problems concerning the detection of heterologously expressed membrane proteins, solubilisation and evaluation of detergent properties, ion exchange chromatography and gel filtering in the presence of a detergent, and control of the protein's stability and integrity after purification. (lu.se)

The results demonstrated that the levels of mRNA transcription and protein expression of the TJ‑associated proteins, claudin‑5, occludin and ZO‑1, were significantly reduced following TBI. (spandidos-publications.com)
Rel frequently combines with other related proteins (NF-KAPPA B, I-kappa B, relA) to form heterodimers that regulate transcription. (bvsalud.org)

Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. (novusbio.com)
Heat shock family of proteins (HSPs) is proposed to play pivotal role in breast tumor development owing to their intrinsic molecular chaperone properties [ 5 ]. (biomedcentral.com)
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. (yeastgenome.org)
120 credits) in Chemistry and Molecular Biology and compulsory for a degree of Master of Science (120 credits) in Protein Science. (lu.se)
The main aim of the course is to enable students to acquire specialised knowledge and understanding of membrane biochemistry and the molecular structure, topology and functional mechanisms of membrane proteins. (lu.se)
A number of proteins from each process, for which the structure is known, are explored in greater detail in order to highlight the functional molecular mechanisms. (lu.se)

All recognized mutations for AD are associated with increased deposition of amyloid-beta (Abeta), a peptide fragment comprising 39-43 amino acids that derive from the catabolism of the amyloid precursor protein (APP) molecule. (medscape.com)

But researchers have also found a causal connection between overexpression of MDMX and MDM2 and breast cancer. (cuny.edu)
To demonstrate a causal relationship where protein A leads to increase in protein B, you need to experimentally increase the level of A and measure if B increases. (stackexchange.com)

Commonly used protein production systems include those derived from bacteria, yeast, baculovirus/insect, mammalian cells, and more recently filamentous fungi such as Myceliophthora thermophila. (wikipedia.org)

Fanning AS, Mitic LL and Anderson JM: Transmembrane proteins in the tight junction barrier. (spandidos-publications.com)
Determination of the transmembrane topology of a protein starts with a model of the protein based on sequence information and theoretical methods. (lu.se)

Download DNA or protein sequence, view genomic context and coordinates. (yeastgenome.org)

This creates a feedback loop that regulates both the activity of p53 protein and the expression of the mdm2 gene (2, 3) . (aacrjournals.org)

In particular, the value of individual protein overexpression as well as combined p53/mdm2 positivity was evaluated because both proteins are functionally connected, and their expression is controlled by an autoregulatory feedback loop. (aacrjournals.org)
The present dataset was collected using a technology platform we recently developed, which overcame several technical challenges in quantifying individual protein turnover rates on a proteome scale. (nature.com)

In the present study, we aimed to investigate whether protein arginine methyl transferase 1(PRMT1) is involved in PXR - activated overexpression of MDR1 during acquired multidrug resistant. (oncotarget.com)
The mRNA and protein levels of MDR1 were detected by real-time PCR and western blotting analysis. (oncotarget.com)
Those results suggest that PRMT1 may be involved in PXR-activated overexpression of MDR1 in resistant breast cancer cells, and AMI-1 may suppress MDR1 by disrupting the interaction between PRMT1 and PXR. (oncotarget.com)

Overexpression is an abnormally and excessively high level of gene expression which produces a pronounced gene-related phenotype. (wikipedia.org)

Whereas p53 and mdm2 positivity correlated significantly ( P = 0.00004), no correlation could be found between mdm2 protein overexpression and tumor stage, lymph node involvement, and presence of distant metastases. (aacrjournals.org)

NEW YORK, January 30, 2019 - The MDMX and MDM2 proteins play critical roles in keeping the tumor-suppressing protein p53 from damaging the production of healthy cells. (cuny.edu)
With this research, we now know that the MDMX protein may be one of the drivers that makes this form of cancer so aggressive, and we can potentially look at targeting this and the MDM2 protein to prevent these cancers from spreading. (cuny.edu)
In univariate analysis, there was a statistically significant correlation between p53 and mdm2 overexpression in the same tumor and poor survival( P = 0.00179). (aacrjournals.org)
Multivariate analysis revealed that coincident mdm2/p53 overexpression, the presence of distant metastases,and tumor grade were independent predictors for tumor progression. (aacrjournals.org)
The present study was undertaken to find out whether there is a correlation between mdm2 and p53 overexpression and the prognostic value of mdm2 positivity with or without p53 protein overexpression. (aacrjournals.org)

Because proteostatic events often trigger zero net change in protein abundance but instead alter protein temporal dynamics 11 - 13 , they typically elude conventional experiments that measure only the steady-state abundance of proteins. (nature.com)

E. coli strain BL21 and BL21(DE3) are two strains commonly used for protein production. (wikipedia.org)
Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein. (yeastgenome.org)

This risk assessment was not dependent on the type of adju- decoding patterns of immunoregulatory serum proteins could re- vant therapy received by the patients. (lu.se)

In contrast to steady-state protein abundance, which may be quantified directly in mass spectrometry (MS) by spectral intensity 16 , 17 or sampling frequency 18 , 19 , protein turnover rates cannot be predicted from steady-state data 20 , requiring instead methods that can distinguish old and new protein molecules in mass spectra 11 , 21 . (nature.com)
METHODS: Overexpression of human UCP-2 was performed in primary cultured human VSMCs (HVSMCs) via adenovirus-mediated gene transfer. (cims-ops.cz)

Hence, multi-domain eukaryotic proteins expressed in bacteria often are non-functional. (wikipedia.org)

Overexpression of the Rieske FeS protein of the Cytochrome b 6 f complex increases C4 photosynthesis in Setaria viridis. (essex.ac.uk)

This is followed by experimental determination using genetic construction and expression of a fusion protein of the membrane protein and a marker protein in a bacterial system which is subsequently analysed. (lu.se)

Key proteins in mitochondria and metabolic pathways are encompassed, in addition to contractile machineries and sarcolemmal signaling proteins. (nature.com)

PIP5K1α was associated with poor patient outcome in triple-negative BC (for PIP5K1α protein, p = 0.011 and for mRNA expression, p = 0.028, log-rank test). (lu.se)

Immunohistochemistry was used to assess ABCC3 protein overexpression. (lu.se)

The history of protein dynamics traces back to 1935, when Schoenheimer and Rittenberg synthesized the first isotopologs of biological molecules to demonstrate the continuous renewal of proteins throughout life 14 , 15 . (nature.com)
An individually planned and executed minor project during two weeks, in which the students express a membrane protein of their choice and demonstrate in some way that the expression was successful. (lu.se)

Thus, human tau overexpression that results in the development of neurofibrillary tau pathology can model authentic human neurodegenerative tauopathies, whereas there is no clear human counterpart, disease or otherwise, of tau overexpression alone. (alzforum.org)

The protein level of TLR4 was significantly increased and the ratio of phosphorylated NF-κB p65 versus p65 subunit was also elevated in PM 2.5 group. (medsci.org)

Or protein localization, complementation of mutants, and activation of signaling. (gardos-channel.com)

Anatomy23

Organisms14

Diseases20

Chemicals and Drugs97

Analytical, Diagnostic and Therapeutic Techniques and Equipment38

Phenomena and Processes63

Disciplines and Occupations1

Information Science3

Health Care3

Recombinant5

Lysate4

Heat-shock Pr2

Kinase5

Vectors2

Coding sequence1

Metastasis4

Genes9

Proto-oncogene2

Photosynthetic2

HER22

Plasmid1

Antibodies1

Group of proteins2

Viral proteins1

Consistent1

MAPK1

Upregulation1

Subcellular1

CDNA3

Green fluores3

Vivo5

Mice5

Cellular4

Promoter4

Accumulation2

Nuclear4

Apoptosis2

Receptor5

Cell8

Purification2

Transcription2

Molecular6

Amyloid1

Causal2

Mammalian1

Transmembrane2

Genomic1

Regulates1

Individual protein2

MDR13

Gene expression1

Lymph1

MDM25

Typically1

Strains2

Patterns1

Methods2

Functional1

Increases1

Genetic1

Metabolic1

MRNA expression1

Immunohistochemistry1

Demonstrate2

Results1

Subunit1

Mutants1