• Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Neural Cell Adhesion Molecule (NCAM) in serum, plasma, tissue homogenates and other biological fluids. (srbiosystem.com)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Neural Cell Adhesion Molecule (NCAM) in samples from Serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species. (srbiosystem.com)
  • Edelman's Nobel Prize-winning research concerned discovery of the structure of antibody molecules. (academicinfluence.com)
  • BioVision's ToxOutTM Protein G (Sepharose) Spin Antibody Purification Kit is a simple, ready to use kit for endotoxin-free IgG purification and immunoprecipitation. (transcriptionfactor.org)
  • This antibody recognizes two proteins (185kDa & 145kDa), identified as two isoforms of neural cell adhesion molecule (NCAM/CD56). (teomics.com)
  • 1 2 Cell surface antigens CD38 and CD138 can be used to distinguish normal cells from clonal plasma cells, but more extensive use of immunophenotyping has been limited by a lack of universally accepted markers of MM. 3-5 Once diagnosed, the current standard-of-care for MM includes immunomodulatory drugs, proteasome inhibitors, steroids, and antibody therapies. (bmj.com)
  • Osteonectin expression was evaluated using immunohistochemistry with anti-secreted protein acidic and rich in cystein (anti-SPARC) antibody. (bvsalud.org)
  • Clinical significance of L1CAM expression and its biological role in the progression of oral squamous cell carcinoma. (nih.gov)
  • The L1CAM gene provides instructions for producing the L1 cell adhesion molecule protein (shortened to L1 protein), which is found on the surface of nerve cells (neurons) throughout the nervous system. (medlineplus.gov)
  • The L1CAM gene mutations that cause this condition lead to an L1 protein that cannot facilitate cell-cell adhesion or participate in various neuronal functions. (medlineplus.gov)
  • Some L1CAM gene mutations result in the production of a protein that is abnormally short and nonfunctional or result in a complete absence of protein. (medlineplus.gov)
  • It is likely that the L1CAM gene mutations that cause these features contribute to disruption of the normal migration and function of nerve cells in the brain and intestine, leading to hydrocephalus and Hirschsprung disease. (medlineplus.gov)
  • The initial event in the replicative cycle of a virus is its interaction with receptors present on the surface of a cell. (medscape.com)
  • Cell surface receptors containing an immunoglobin domain. (embl.de)
  • Killer cell inhibitory receptors. (embl.de)
  • Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. (arigobio.com)
  • Glycosylation of classical death receptors fine-tunes cell death programs. (nature.com)
  • T-cell development ensures the formation of diverse repertoires of T-cell receptors (TCRs) that recognize a variety of antigens. (for2953-sia.de)
  • Moreover, platelets are widely considered "circulating neurons" because of the similarities existing between the two cells in terms of enzymes, receptors, and metabolic products [ 10, 11 ]. (pharmaceuticalintelligence.com)
  • Using immunohistochemical techniques, we have examined the effects of cortical lesions induced either by aspiration of the frontoparietal cortex or by thermocoagulation of pial blood vessels on axonal and glial molecules associated with neuronal plasticity in the striatum. (jneurosci.org)
  • Immunostaining for basic fibroblast growth factor (bFGF) markedly decreased in striatal astrocytes after both lesions, despite an increased staining for glial fibrillary acidic protein (GFAP). (jneurosci.org)
  • These effects differed from those observed after partial denervation of the hippocampus and spinal cord, revealing marked regional differences in the response of axonal and glial proteins to afferent lesions. (jneurosci.org)
  • Anti-NCAM1 autoantibody disrupts both NCAM1-NCAM1 and NCAM1-glial cell line-derived neurotrophic factor (GDNF) interactions. (neurosciencenews.com)
  • Each of the cell lines also reacted with antibodies against neural cell adhesion molecules, but none of them were positive for antibodies against glial fibrillary acidic protein, keratin, microtubule-associated protein tau and microtubule-associated protein 2, human lymphocyte antigen-DR, epidermal growth factor receptor, and T-cell antigen. (duke.edu)
  • In summary, the detection of neurofilament proteins and synaptophysin and the absence of glial fibrillary acidic protein provide strong evidence for a neuronal phenotype of D384 Med, D425 Med, and D458 Med. (duke.edu)
  • Janeczko K. The proliferative response of S-100 protein-positive glial cells to injury in the neonatal rat brain. (amarpharma.com)
  • Schmidt-Kastner R, Szymas J. Immunohistochemistry of glial fibrillary acidic protein, vimentin and S-100 protein for study of astrocytes in hippocampus of rat. (amarpharma.com)
  • Li Q, Cao Z, Zhao S. The Emerging Portrait of Glial Cell Line-derived Neurotrophic Factor Family Receptor Alpha (GFRα) in Cancers. (medsci.org)
  • Glial cell line-derived neurotrophic factor family receptor alpha (GFRα) members have been widely connected to the mechanisms contributing to cell growth, differentiation, cell migration and tissue maturation. (medsci.org)
  • The glial cell line-derived neurotrophic factors (GDNFs), a family of neurotrophic factors, were initially thought to be able to regulate the growth, survival, and differentiation of neural-derived cell types. (medsci.org)
  • Le Gal La Salle G, Rougon G, Valin A. The embryonic form of neural cell surface molecule (E-NCAM) within the rat hippocampus and its reexpression on glial cells following kainic acid-induced status epilepticus. (glob3.com)
  • Our objective is to unravel fundamental dynamic neural and glial mechanisms underlying learning-related altered neural network structures and information processing in conscious individuals using implantable and biocompatible nanostructured neural interfaces. (lu.se)
  • The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. (arigobio.com)
  • Biomolecule/Target: N/ASynonyms: S1 Protein, Spike glycoprotein subunit1, S glycoprotein subunit1, SARS-CoV-2 S1 ProteinAlternates names: S1 Protein, Spike glycoprotein subunit1, S glycoprotein. (transcriptionfactor.org)
  • 10 Galectins can function inside the cells by modulating signaling pathways, 11 although they also act extracellularly by establishing multivalent interactions with cell surface glycans and delivering signals that lead to disruption of cellular homeostasis. (nature.com)
  • Cell adhesion molecules are known to play a pivotal role in assembling neural circuits. (nih.gov)
  • A nanoscale modular design strategy was employed to synthesize six engineered, recombinant proteins intended to mimic aspects of the extracellular matrix proteins fibronectin, laminin, and elastin as well as the cell-cell adhesive protein neural cell adhesion molecule. (frontiersin.org)
  • The proteins are synthesized using recombinant, genetic engineering techniques, allowing for the creation of biocompatible polymers with nanoscale precision that impart highly specific protein functionalities. (frontiersin.org)
  • We make use of a highly flexible multi-step cloning strategy in order to allow for the rapid synthesis of new recombinant proteins that can introduce many different biofunctionalities. (frontiersin.org)
  • No. 6510) is a genetically engineered protein containing three Ig-binding regions of native Protein G. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein G to ensure the maximum specific IgG binding. (transcriptionfactor.org)
  • Protein G-Sepharose beads (Cat# M1301) for IgG purification contain covalently coupled recombinant Protein G to 6% cross-linked Sepharose beads, the most popular resin for protein purification. (transcriptionfactor.org)
  • Objective: This study evaluated the osteogenic induction of human bone marrow cells by human recombinant bone morphogenetic protein-4 (rhBMP-4) and proteins released by Saos-2 (human osteosarcoma cell line). (bvsalud.org)
  • Previous research has suggested that neural cell adhesion molecule (NCAM1), which helps cells in the brain talk to one another via specialized connections known as synapses, may have a role in the development of schizophrenia. (neurosciencenews.com)
  • NCAM1 is induced only in green cells (HeLa cells). (neurosciencenews.com)
  • Serum from patients with anti-NCAM1 autoantibody react only to green cells (framed in red). (neurosciencenews.com)
  • Many of these PSA-NCAM immunoreactive cells also express doublecortin, a molecule involved in neuronal migration. (nih.gov)
  • These results expand the current knowledge of the neuronal populations expressing PSA-NCAM in the piriform cortex, suggesting that some of these cells could be involved in structural plastic events such as axonal outgrowth, synaptogenesis or even neuronal migration. (nih.gov)
  • This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. (arigobio.com)
  • Regarding animal models in ASD, many studies focus on gene expression, cortical neuronal migration and cell maturation, and neural network deficits. (bvsalud.org)
  • Kinetics of homophilic binding by embryonic and adult forms of the neural cell adhesion molecule. (academicinfluence.com)
  • Chemical characterization of a neural cell adhesion molecule purified from embryonic brain membranes. (academicinfluence.com)
  • The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)
  • 2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)
  • Herein we show the expression of polySia on hematopoietic progenitor cells, and demonstrate a role for this glycan in immune response using both acute inflammatory and tumor models. (ca.gov)
  • 1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)
  • In a study published last month in Cell Reports Medicine , researchers from Tokyo Medical and Dental University (TMDU) have identified an autoantibody-a protein that is produced by the immune system to attach to a specific substance from the individual's own body, rather than to a foreign substance like a virus or bacteria-in some patients with schizophrenia. (neurosciencenews.com)
  • Association of NrCAM Protein in an Iranian Sample of Patients with Schizophrenia', Biomacromolecular Journal , 6(2), pp. 155-162. (bmmj.org)
  • Ethanol interacts with an alcohol binding pocket in the L1 extracellular domain (ECD), and dephosphorylation of S1248 in the L1 cytoplasmic domain (CD) renders L1 adhesion insensitive to inhibition by ethanol (L1 insensitive). (ox.ac.uk)
  • A breakthrough for the identification of the proteome of the presynaptic active zone was the successful employment of antibodies directed against a cytosolic epitope of membrane integral synaptic vesicle proteins for the immunopurification of synaptic vesicles docked to the presynaptic plasma membrane. (mdpi.com)
  • Combining immunopurification and subsequent analytical mass spectrometry, hundreds of proteins, including synaptic vesicle proteins, components of the presynaptic fusion and retrieval machinery, proteins involved in intracellular and extracellular signaling and a large variety of adhesion molecules, were identified. (mdpi.com)
  • Therefore, this engineered protein adsorption approach allows for the facile preparation of tunable, quantifiable, and reproducible surfaces for in vitro studies of cell-ligand interactions and for potential application as coatings on neural implants. (frontiersin.org)
  • Of specific interest is the role that nanoscale cell-surface interactions play in the biocompatibility of neural implants. (frontiersin.org)
  • Two decades later, pioneering studies suggested that lectin-like molecules constitutively expressed on the surface of macrophages can selectively recognize changes on glycans decorating the surface of apoptotic thymocytes, 4 , 5 although these studies likewise did not provide substantial insight into the mechanisms by which lectin-glycan interactions regulate cell death. (nature.com)
  • We discuss here the contribution of glycan-lectin interactions to the initiation, execution and resolution of apoptosis and their emerging roles in other cell death programs including autophagy. (nature.com)
  • CD117 by immunohistochemical methods sion molecules, involved in cell-cell and in order to clarify the role of the infiltrating cell matrix interactions and thought to take inflammatory cells in the pathomechanisms part in cell motility [ 2,3 ]. (who.int)
  • Cell adhesion molecule involved in a diverse range of contact-mediated interactions among neurons, astrocytes, oligodendrocytes, and myotubes. (bvsalud.org)
  • We strive for a detailed understanding of the interactions between cells and nanostructures with respect to cell behavbehaviourl physiology and cell mechanics. (lu.se)
  • Description: A sandwich ELISA kit for detection of Neural Cell Adhesion Molecule from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (srbiosystem.com)
  • Objective: This investigation intended to find significant changes in NrCAM protein expression in blood serum of SZ patients. (bmmj.org)
  • Another purpose of this investigation is to evaluate the association of variant rs10235968 with NrCAM protein expression in patients' serum. (bmmj.org)
  • Results: NrCAM protein expression in blood serum has shown a significant difference between patients and healthy group (P = 0.0509). (bmmj.org)
  • Immunoplates for Exosome capture and quantification from human biofluids (plasma, serum, urine, saliva) and from human or mouse cell media. (transcriptionfactor.org)
  • This kit can rapidly purify IgG from serum, ascites and cell culture media from various species such as human, mouse, rat, goat and rabbit. (transcriptionfactor.org)
  • Initial efforts focused on the optimization and development of assays based on cultured endothelial cells or isolated vascular tissue responses to whole serum, which represents a cumulative balance of anti- and pro-inflammatory factors. (cdc.gov)
  • Primary cerebrovascular endothelial cells treated with serum from MWCNT-7-exposed WT mice exhibited significantly reduced nitric oxide (NO) generation, as measured by electron paramagnetic resonance, an effect that was independent of NO scavenging. (cdc.gov)
  • In vitro, serum from MWCNT exposed mice was able to induce expression of adhesion molecules at both the gene and protein level. (cdc.gov)
  • Treatment of endothelial cells with serum from MWCNT-exposed mice in a wound-healing assay resulted in decreased cell motility and cytokinesis, also consistent with mRNA expression patterns in microarray analysis. (cdc.gov)
  • When considering possible autoantibodies that might cause schizophrenia, the research team had a specific protein in mind. (neurosciencenews.com)
  • They also had fewer synapses and dendritic spines, which are structures that are important for the connections between brain cells, and are also affected in schizophrenia. (neurosciencenews.com)
  • Also, it has been proven that, malfunctions in the structure of neocortical cells associated with schizophrenia [2]. (bmmj.org)
  • This suggests a model in which intracellular motor proteins move the TGN organelles along the intracellular sides of the neurites, pulling NCAM across the extracellular surface of the membrane at the same time. (rupress.org)
  • They play a pivotal role in many physiological processes, including membrane remodelingand cell signaling [ 1, 2 ], and are involved in neurodegenerative disorders [ 3, 4 ]. (pharmaceuticalintelligence.com)
  • The L1 protein spans the cell membrane, with one end of the protein inside the cell and the other end projecting from the outer surface of the cell. (medlineplus.gov)
  • Understanding the function of lectin-glycan recognition systems in cell death will facilitate the implementation of novel therapeutic strategies aimed at controlling unbalanced cell proliferation and survival in several pathologic conditions. (nature.com)
  • Determined by its ability to increase the proliferation effect of IL-2 in murine CTLL-2 cells. (transcriptionfactor.org)
  • Neural cell adhesion molecule (N-CAM) inhibits astrocyte proliferation after harm to totally different regions of the adult rat brain. (glob3.com)
  • Anti-human lymphocyte antigen-A,B and anti-beta 2-microglobulin antibodies reacted with xenografts of D384 Med and D425 Med and were weakly positive for a small population of D384 Med cultured cells. (duke.edu)
  • IL-2R has a high binding affinity to IL-2 and is expressed by antigen-activated T lymphocytes (T cells). (transcriptionfactor.org)
  • IL-2 Rα is also known as CD25, p55, and Tac (activated T cell) antigen. (transcriptionfactor.org)
  • Glycosylation is a major posttranslational modification present in virtually all cells, including T-lymphocytes, that regulates activity/functions. (for2953-sia.de)
  • Interleukin-2 receptor (IL-2R) is a heterotrimeric protein expressed on the surface of certain immune cells, such as lymphocytes, that binds and responds to the cytokine IL-2. (transcriptionfactor.org)
  • Le pourcentage de CD44 dans les lymphocytes T périphériques était significativement plus élevé chez les patients que chez les témoins, comme détecté par la cytométrie en flux. (who.int)
  • Methods In this study, we first used a mass spectrometry (MS)-based discovery-driven cell surface capture (CSC) approach to map the cell surface N -glycoproteome of MM cell lines. (bmj.com)
  • The expression of known [COL22A1 (collagen XXII), NCAM (neural cell adhesion molecule), POSTN (periostin), NES (nestin), OSTN (musclin/osteocrin)] and previously undescribed [MNS1 (meiosis-specific nuclear structural protein 1), and LCT (lactase)] MTJ genes was confirmed at the protein level by immunofluorescence on tissue sections of human MTJ. (uni-koeln.de)
  • Genes down-regulated in comparison of B cells versus NK cells. (gsea-msigdb.org)
  • El objetivo de nuestro estudio tuvo como objetivo revisar y correlacionar genes involucrados en TEA y aquellos relacionados con la comunicación ultrasónica en estudios con modelos animales de comportamiento social basado en el lenguaje en la base de datos PubMed. (bvsalud.org)
  • At a developing contact site, the NCAM molecules of the two cells cluster, trapping the TGN organelles and stabilizing the nascent synapse. (rupress.org)
  • Glycans, either alone or complexed with glycan-binding proteins, can deliver intracellular signals or control extracellular processes that promote initiation, execution and resolution of cell death programs. (nature.com)
  • What is the precise role of intracellular and extracellular galectins in the control of cell death programs? (nature.com)
  • But how do the cells coordinate the extracellular formation of a contact with the intracellular movement of organelles? (rupress.org)
  • describe the interaction of neural cell adhesion molecule (NCAM) clusters with intracellular organelle aggregates derived from the trans-Golgi network (TGN). (rupress.org)
  • The work is the first demonstration that recognition molecules such as NCAM directly link extracellular signals to intracellular organelle movement at developing synapses. (rupress.org)
  • Tudor-related proteins TDRD1/MTR-1, TDRD6 and TDRD7/TRAP: domain composition, intracellular localization, and function in male germ cells in mice. (shengsci.com)
  • Numerous proteins regulating the rearrangement of the cytoskeleton are indicative of the functional and structural dynamics of the presynapse. (mdpi.com)
  • At that time, glycobiology, which is the study of carbohydrates and their recognition by motif-specific carbohydrate-binding proteins or lectins, lagged far behind the studies that defined the structural and cellular biology of cell death. (nature.com)
  • They serve as recognition molecules between corresponding synaptic partners. (nih.gov)
  • Immunoelectron microscopic localization of neural cell adhesion molecules (L1, N-CAM, and MAG) and their shared carbohydrate epitope and myelin basic protein in growing sciatic nerve. (glob3.com)
  • Administration of NMDA receptor antagonists upregulates the expression of the polysialylated form of the neural cell adhesion molecule (PSA-NCAM) in the adult hippocampus. (nih.gov)
  • Polysialic acid (polySia) is a large glycan with restricted expression, typically found attached to the protein scaffold neural cell adhesion molecule (NCAM). (ca.gov)
  • Specifically, we found that human NK cells modulate expression of NCAM and the degree of polymerization of its polySia glycans according to activation state. (ca.gov)
  • This contrasts with the mouse, where polySia and NCAM expression are restricted to multipotent hematopoietic progenitors and cells developing along a myeloid lineage. (ca.gov)
  • This is the first demonstration of polySia expression and regulation on myeloid cells, and the results in animal models suggest a role for polySia in immune regulation. (ca.gov)
  • Protein Expression and Refolding, Protein Engineering. (uc.edu)
  • To evaluate protein expression, ELIZA method was used. (bmmj.org)
  • The association of variant rs10235968 and NrCAM protein expression has not detected (P = 0.9468). (bmmj.org)
  • Altered expression of neuronal cell adhesion molecules induced by nerve harm and restore. (amarpharma.com)
  • However, abnormal expression or aberrant activation of these molecules may convert normal growth signals to undesirable signals inducing overgrowth, becoming an important contributor to a variety of human cancers. (medsci.org)
  • Nine of these proteins were identified as potential immunotherapeutic targets, including five that were validated by FCM, confirming their expression on the cell surface of primary MM patient cells. (bmj.com)
  • Cell adhesion molecules in the regulation of animal form and tissue pattern. (academicinfluence.com)
  • Based on whether it cooperates with the second receptor RET, GFRα has also been widely linked to the mechanisms that contribute to cell growth, differentiation and migration and tissue maturation. (medsci.org)
  • The surface of the living cells is decorated by a complex layer of glycosylated molecules that store relevant biological information. (nature.com)
  • Kenwrick S, Watkins A, De Angelis E. Neural cell recognition molecule L1: relating biological complexity to human disease mutations. (medlineplus.gov)
  • Phosphorylation of Y1176 and ankyrin-G coupling with L1 are higher in NIH/3T3 clonal cell lines in which ethanol inhibits L1 adhesion than in ethanol-resistant NIH/3T3 clonal cell lines. (ox.ac.uk)
  • As confirmation that ligand density in these engineered systems impacts neuronal cell behavior, we demonstrate that increasing the density of fibronectin-derived RGD ligands on coated surfaces while maintaining uniform protein surface coverage results in enhanced neurite extension of PC-12 cells. (frontiersin.org)
  • Thus, the role of GFRα is more complicated than originally assumed, and it is necessary to revisit and review the role played by this versatile molecule in tumors. (medsci.org)
  • It is also expressed on some mesodermally derived tumors such as rhabdomyosarcoma and also on natural killer cells. (teomics.com)
  • It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH. (teomics.com)
  • ENM inhalation studies have identified several potential systemic outcomes that are relevant to human health, including the development or exacerbation of inflammatory vascular disease / atherosclerosis, vasoconstriction, and neuropathies, possibly related to abnormal signaling in endothelial cells. (cdc.gov)
  • Pemphigus vulgaris (PV) is a life-threate- disorders and to shed a light on the role of ning blistering skin disease in which pa- mast cells in autoimmune diseases [ 7 ]. (who.int)
  • En 1999 me incorporé a la Universidad de Navarra, en la cual he desarrollado mi labor investigadora hasta la actualidad. (unav.edu)
  • Endothelial cells as biosensors for occupational cardiovascular risk. (cdc.gov)
  • The results show that spectrin links the TGN organelles directly to clusters of NCAM, and that NCAM is one of the first proteins to accumulate at sites where neurites contact each other. (rupress.org)
  • fication and isolation via fluorescence-activated cell sort- ing (FACS). (lu.se)
  • These putative migratory cells and some neurogliaform neurons in layer II do not express NeuN, a marker of differentiated neurons. (nih.gov)
  • This positioning allows the L1 protein to attach (bind) to other proteins, including other L1 proteins, on neighboring neurons to help these cells stick to one another (cell-cell adhesion). (medlineplus.gov)
  • The L1 protein plays a role in the movement (migration) and organization of neurons and the outgrowth of axons, which are specialized extensions of neurons that transmit nerve impulses. (medlineplus.gov)
  • The protein also plays a role in the formation of the protective sheath (myelin) that surrounds certain neurons and the formation of junctions between nerve cells (synapses), where cell-to-cell communication occurs. (medlineplus.gov)
  • The GDNF family ligands (GFLs) function through a glycosyl-phosphatidylinositol-(GPI) anchored coreceptor, GDNF family receptor alpha (GFRα), and rearranged during transfection (RET), a well-known receptor tyrosine kinase involved in kidney development, spermatogonial stem cell maintenance, and the development and maintenance of the sympathetic, parasympathetic, and enteric nervous systems [ 1 , 2 ]. (medsci.org)
  • CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
  • Stem Cell Reports. (lu.se)
  • Development: For advances in developmental biology and stem cells. (lu.se)
  • Undifferentiated mesenchymal stem cells are pluripotent cells that can differentiate into various phenotypes and are a source of osteogenic Cells 1,2 . (bvsalud.org)