Protein murine leukemia cell line. Medical search. Frequent questions

It was identified as a gene rearranged and over-expressed in the murine lymphoma LSTRA, most likely as a result of the insertion of Moloney murine leukemia virus DNA immediately adjacent to the gene (1). (bio-techne.com)

Mutant line R3-2 exhibited a nearly 1000-fold resistance to infection by genetically marked Moloney murine leukemia virus genomes, and was resistant to pseudotyped viruses utilizing the ecotropic envelope, the amphotropic envelope, or even the VSV G envelope protein. (biomedcentral.com)

Altered expression of this gene or a change in the subcellular localization of its protein is associated with some cases of acute myeloid leukemia and colorectal cancer. (wikipedia.org)
Overexpression of HOXB6, along with the addition of MEIS1 protein, has been implicated in the development of acute myeloid leukemia (AML). (wikipedia.org)
Acute myeloid leukemia is a cancer of the blood cells, specifically the leukocytes. (wikipedia.org)
The expanded NK cells were significantly more potent than unstimulated or IL-2-stimulated NK cells against acute myeloid leukemia cells in vitro . (aacrjournals.org)
1 month when injected into immunodeficient mice and could eradicate leukemia in murine models of acute myeloid leukemia. (aacrjournals.org)

B lymphoma Moloney murine leukemia virus insertion region 1 homolog (BMI-1) is an oncogene which functions by regulating P16 and P19 cell cycle inhibitor genes and is also associated with erythroplakia and tongue cancer. (cancertools.org)
This gene is also a translocation partner in anaplastic large cell lymphoma and inflammatory myofibroblastic tumor cases, where a t(2;17)(p23;q25) translocation has been identified with the anaplastic lymphoma kinase (ALK) gene on chromosome 2, and a t(8;17)(q24;q25) translocation has been identified with the MYC gene on chromosome 8. (cancerindex.org)
Many tumor microenvironments, including lymphoma and carcinoma, are enriched in immune suppressive cells that contribute to immune exhaustion by means expression of inhibitory ligands, suppressive cytokines, and tumor-promoting factors. (researchgate.net)
Leukemic presentation of diffuse large B-cell lymphoma: an unusual pattern associated with splenic involvement. (rochester.edu)
Animal models for human tumour mental animals is not easy to answer does induce adult T-cell leukaemia/ viruses that make use of animal virus- for these agents, because cancer bi- lymphoma (ATLL), albeit in monkeys es are scarce. (who.int)
genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)

HOX B6 gene is only expressed in erythoid progenitor cells, which are the precursor to red blood cells used for transport of oxygen and carbon dioxide throughout the body. (wikipedia.org)
In the injured brain, the behavior of neural stem/progenitor cells (NSCs) is regulated by multiple converging factors encountered in the niche, which is composed of several neural and non-neural cell types. (omeka.net)
3 Detection of the MLL-AF4 fusion protein in CD34 + /CD19 − fetal hematopoietic stem or progenitor cells or in fetal cells before hematologic specification suggests that there is a window of opportunity during development for the MLL-AF4 fusion protein to immortalize hematopoietic stem and progenitor cells (HSPCs). (ashpublications.org)
[ 5 , 6 ] Furthermore, experiments have demonstrated that epidermal progenitors in the touch domes are capable of producing Merkel cell lines and that epithelial progenitor populations in adults have the capacity to give rise to both neuroendocrine and squamous lineages. (medscape.com)
Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
To varying degrees, these fates also extend to the Such state stability is required in stem and progenitor cells to immediate progeny of stem cells, known as progenitor or support self-renewal and maintenance of the uncommitted transit-amplifying cells. (lu.se)
A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)
1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)

pMIRH-130b mice presented with a mixed B-cell/myeloid malignancy, whereas pMIRH-128a mice developed pro-B-cell ALL. (ashpublications.org)
Further transplantation experiments demonstrated that lympho-myeloid progenitors (LMPPs) allowed the propagation of pMIRH-130b mixed B-cell/myeloid leukemia, whereas Lin-Sca1 +/− Il7 + Kit + blasts maintained pro-B-ALL disease of pMIRH-128a mice. (ashpublications.org)
PD-1 is expressed on activated T cells, B cells, and myeloid cells. (biomedcentral.com)

RNA was generated using Moloney murine leukemia virus (M-MLV) reverse transcriptase (Promega). (eviematilda.art)

BMI-1 is thought to repress, p16(Ink4a), a cyclin-dependent kinase inhibitor and tumor suppressor that induces cell cycle arrest at the Gap 1 phase. (cancertools.org)
PAX5-JAK2 encodes a protein consisting of the PAX5 DNA-binding region fused to the constitutively active JAK2 kinase domain. (bvsalud.org)
The DNA-binding function and kinase activity of Pax5-Jak2 as well as IL-7 signaling contributed to leukemia development. (bvsalud.org)
A number of human cancer cell lines show over-expression of Lck, pointing to a possible role for this kinase in the initiation and maintenance of the transformed state in human cancers (2). (bio-techne.com)
Overexpressed Down Syndrome Cell Adhesion Molecule (DSCAM) Deregulates P21-Activated Kinase (PAK) Activity in an In Vitro Neuronal Model of Down Syndrome: Consequences on Cell Process Formation and Extension. (cnrs.fr)
p21-Activated kinase 3 (PAK3) protein regulates synaptic transmission through its interaction with the Nck2/Grb4 protein adaptor. (cnrs.fr)

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. (bio-techne.com)
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. (bio-techne.com)
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (bio-techne.com)
In addition, we used an ELISA based on recombinant XMRV proteins to screen 146 PCa patient sera for viral Env- and Gag-specific antibodies. (biomedcentral.com)

The MRD levels were monitored by detection of clone-specific rearrangements of the immunoglobulin and T-cell receptor genes applying a quantitative polymerase chain reaction-based technique. (bvsalud.org)
The suppressor genes were then recovered from these virus sensitive cells, and their ability to restore virus susceptibility was confirmed by reintroduction of these cDNAs into the resistant line. (biomedcentral.com)
It is becoming increasingly apparent that mammalian cells harbor numerous genes that induce intracellular blocks to retrovirus infection [ 1 , 2 ]. (biomedcentral.com)
We have been involved in the development of screens and selections for virus resistance genes, and have isolated mutant cell lines after chemical mutagenesis that are profoundly resistant to retrovirus infection. (biomedcentral.com)
To probe the nature of the blocks in these mutant cell lines, we have sought to identify and characterize suppressor genes that override the restriction exhibited by these cells. (biomedcentral.com)
TCL1 and TCL1b genes on human chromosome 14q23.1 are activated in T cell leukemias by translocations and inversions at 14q32.1, juxtaposing them to regulatory elements of T cell receptor genes. (unife.it)
In addition to TCL1 and TCL1b, the human locus contains two additional genes, TCL1-neighboring genes (TNG) 1 and 2, encoding proteins of 141 and 110 aa, respectively. (unife.it)
To aid in the development of a mouse model we also have characterized the murine Tcl1 locus and found five genes homologous to human TCL1b. (unife.it)
Yet another group of genes associated with cancer formation regulates programmed cell death, or apoptosis. (the-scientist.com)
The normal function of the proteins expressed by these genes is to recognize superfluous, damaged, aged, or aberrant cells that must be eliminated. (the-scientist.com)
The importance of apoptosis is exemplified by the vertebrate adaptive immune system, in which B lymphocytes, as they differentiate from their precursor cells, rearrange the DNA of immunoglobulin genes to generate a vast number of potential antibodies. (the-scientist.com)
Furthermore, single-cell-based proteomic and transcriptomic analysis of D4-IgG4H-CD28TM CAR T cells reveals specific genes (e.g. (nature.com)
Comparative transcriptomic analysis of tumor cells from diseased mice revealed the expression of several well-known MLL-rearranged leukemia-associated target genes, including Meis1 , Runx1 ( pMIRH-128a mice), HoxA9 , Flt3 ( pMIRH-130b mice), Cdk6 , and Bcl2 . (ashpublications.org)
overexpression of these genes decreased proliferation or induced apoptosis of human MLL-AF4 + ALL cell lines, respectively. (ashpublications.org)
explosion further, consider that a fictitious small genome with 2002) More recently and more dramatically, the potential for 260 genes would host the same number of combinations as cell state conversions is exemplified by the reprogramming of the number of atoms in the visible universe! (lu.se)
Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)

This gene is a member of the Antp homeobox family and encodes a protein with a homeobox DNA-binding domain. (wikipedia.org)
This gene encodes a protein containing a C3HC4-type RING finger domain, which is a specialized type of Zn-finger that binds two atoms of zinc and is thought to be involved in mediating protein-protein interactions. (cancerindex.org)
The human PD-1 gene is located on chromosome 2 at band q37, and the full length PD-1 cDNA is 2,106 nucleotides long and encodes a predicted protein of 288 amino acid residues. (biomedcentral.com)

We recently described the expression of leukemia inhibitory factor (LIF) in human fetal and murine corticotrophs. (manchester.ac.uk)
Targeting of Mll-AF4 in early fetal HSPCs enhanced the lymphoid potential of primed multipotent cells but was insufficient by itself to induce leukemia. (ashpublications.org)
To address the role of these miRNAs in transformation, the investigators overexpressed them in lineage-marker-depleted Sca1 + Kit + (LSK) fetal liver cells from targeted Mll-AF4 invertor mice (see figure). (ashpublications.org)

HOXB6 is a structural protein that has been shown to influence the growth and differentiation of the different blood lineages. (wikipedia.org)
Primary Human bronchial epithelial cells when grown in vitro have a limited lifespan and begin to deviate both in phenotype and morphology, losing the plasticity required around passage 4 or 5, for air-liquid interface (ALI) differentiation. (cancertools.org)
These Human bronchial epithelial cells expressing BMI-1 retain both viability and differentiation potential of wild-type human bronchial epithelium while importantly not demonstrating changes in cell karyotype. (cancertools.org)
Normal human bronchial epithelial cells were grown in a growth factor-supplemented medium and differentiated at ALI in bronchial epithelial differentiation medium. (cancertools.org)
To further promote differentiation and to increase mechanical signaling, Notch signaling was activated by presenting cells with fibronectin and DLL1 protein. (siu.edu)
The differentiation was found to have a pronounced effect in the presence of fibronectin and DLL1 protein together with the withdrawal of LIF. (siu.edu)
The intellectual disability protein PAK3 regulates oligodendrocyte precursor cell differentiation. (cnrs.fr)
Pak3 promotes cell cycle exit and differentiation of β-cells in the embryonic pancreas and is necessary to maintain glucose homeostasis in adult mice. (cnrs.fr)
Ideally, iPSC-based therapies in the future will rely on the isolation of skin fibroblasts or keratinocytes, their reprogramming into iPSCs, and the correction of the genetic defect followed by differentiation into the desired cell type and transplantation. (biomedcentral.com)
Bmi-1 (B cell-specific Moloney leukemia virus integration site-1) was the first gene discovered in the polycomb protein family, and it plays an important role in cell immortalization and differentiation and embryonic stem cell development [1]. (docksci.com)
Based on their functional outcome, co-signaling molecules can be divided as co-stimulators and co-inhibitors, which positively and negatively control the priming, growth, differentiation and functional maturation of a T-cell response. (biomedcentral.com)
Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)
Temporal multimodal single-cell profiling of native hematopoiesis illuminates altered differentiation trajectories with age. (lu.se)

Since it is a transcriptional factor, HOX B6 regulates erythpoigenesis (red blood cell formation) using mRNA as the basis for certain protein productions. (wikipedia.org)
In her paper, " Worse Than The Disease: Reviewing Some Possible Unintended Consequences of mRNA Vaccines Against COVID-19 ," published in the International Journal of Vaccine Theory, Practice and Research in collaboration with Dr. Greg Nigh, 2 Seneff explains that a significant part of the problem is that while the natural spike protein is bad, the spike protein your body produces in response to the vaccine is even worse. (fromthetrenchesworldreport.com)
LIF and the related cytokine oncostatin M induced basal, and corticotropin-releasing hormone (CRH) induced proopiomelanocortin (POMC) mRNA and ACTH secretion in AtT20 cells. (manchester.ac.uk)
Murine PD-1 mRNA expression has been shown to be correlated with activation-induced apoptosis in a mouse T-cell hybridoma cell line and murine thymocytes [ 13 ]. (biomedcentral.com)
tein, and neuronal cell adhesion molecule mRNA were also increased in olfactory bulb. (cdc.gov)

This specificity occurs because only those cells possess the interleukin-5 receptor. (biomedcentral.com)
Heterogeneous antigen expression is a key barrier influencing the activity of chimeric antigen receptor (CAR) T cells in solid tumors. (nature.com)
The reason for this is because the synthetic RNA has been manipulated in such a way as to create a very unnatural spike protein that result in it not collapsing on itself into the cell once it attaches to the ACE2 receptor, as it normally does. (fromthetrenchesworldreport.com)
So, the spike protein binds to the ACE2 receptor once it's produced by the human cell … but it's a modified version of the spike protein. (fromthetrenchesworldreport.com)
Because of this redesign, it can't do that, so it sits there on the ACE receptor, exposed … That allows the immune cells to produce antibodies specific to that place where it should be fusing with the cell, the fusion domain. (fromthetrenchesworldreport.com)
Expanded natural killer cells killed both allogeneic and autologous primary myeloma cells avidly via a perforin-mediated mechanism in which the activating receptor NKG2D, natural cytotoxicity receptors, and DNAX-accessory molecule-1 played a central role. (haematologica.org)
Such immune dysfunction may be due to a disorder in thymic output function (in particular in young patients), which results in a lower level of naive T-cells in the peripheral blood available for an immune response to the proliferation and abnormal expression of the T cell receptor (TCR) repertoire. (biomedcentral.com)

Similar results were obtained with a continuous line of mouse mammary epithelial cells infected with Fried, Moloney, Rauscher, or Kirsten murine leukemia viruses, or Friend virus-related or Moloney virus-related mink cell focus-forming viruses. (elsevierpure.com)
Bmi-1 expression is also associated with the Hedgehog signaling pathway, which regulates of human mammary stem cell self-renewal [6]. (docksci.com)
Acceleration of mouse mammary tumor virus-induced murine mammary tumorigenesis by a p53 172H transgene: influence of FVB background on tumor latency and identification of novel sites of proviral insertion. (rochester.edu)

A leading contributing factor to metastasis and treatment resistance is the heterogeneity and plasticity of the cells within tumors 6 . (nature.com)
Malignant tumors consist of cancer cells and tumor-associated host cells, both participating in invasion and distant metastasis. (biomedcentral.com)
Immune surveillance plays a major role in the defense against virus-associated tumors, where the virally encoded transforming proteins provide readily recognizable foreign targets. (the-scientist.com)
But nonviral tumors, which are composed of aberrant host cells, do not provide such targets, and the immune response is suppressed by defenses against autoimmune reactions. (the-scientist.com)
Bmi-1 is highly expressed in a variety of human tumors, including lung cancer, ovarian cancer, acute myelogenous leukemia, nasopharyngeal cancer, breast cancer, and neuroblastoma, suggesting that Bmi-1 may play an important role in cancer occurrence and development [2-4]. (docksci.com)
Telomerase is excessively activated in most of tumors and is involved in the regulation of cell cycle proteins, such as p16Ink4a and p21WAF1, which induce epithelial cell apoptosis [7]. (docksci.com)
To improve the response rate in B cell malignancies and translate the success of CAR T cells to solid tumors, the optimization of this class of therapeutics is required. (nature.com)
Adoptive transfer of expanded natural killer cells inhibited the growth of established OPM2 and high-risk primary myeloma tumors grown in the murine model. (haematologica.org)
Relevant information has now been established on solid tumors and their micro-environments, which include immune, endothelial and mesenchymal cells. (fortunejournals.com)
Also see Merkel Cell Tumors of the Head and Neck and Merkel Cell Carcinoma and Rare Appendageal Tumors . (medscape.com)
Resolving the Pathogenesis of Anaplastic Wilms Tumors through Spatial Mapping of Cancer Cell Evolution. (lu.se)

HOXB6 has the ability to cause the indefinite proliferation of murine marrow cells, as well as expand hematopoietic stem cells. (wikipedia.org)
Studies have shown that Bmi-1 is important for maintaining proliferation and selfrenewal of neural stem cells, hematopoietic stem cells, and cancer stem cells [3]. (docksci.com)
P16INK4a can directly inhibit cell proliferation by inhibiting Rb phosphorylation and preventing cells from entering S phase (DNA synthesis phase). (docksci.com)
New antitumor strategies using drugs targeted to intracellular signaling involved in cell proliferation and survival, angiogenesis, and metastasis have become promising in recent years. (researchgate.net)
Knockdown of miR-128a and/or miR-130b significantly impaired proliferation of human MLL-AF4 + cell lines, and inhibition of miR-130a significantly impaired disease initiation by the pediatric MLL-AF4 + SEM ALL cell line in immunodeficient NOD- scid IL2Rgamma null (NSG) mice. (ashpublications.org)
Moreover, in adults, Merkel cells undergo slow turnover and are replaced by cells originating from epidermal stem cells, not through the proliferation of differentiated Merkel cells. (medscape.com)
ABSTRACT This study examined haematopoietic stem cells of 19 high-risk cases of myelodysplastic syndrome (MDS) for apoptotic and anti-apoptotic signals and cellular proliferation and correlated these with clinical and cytogenetic subtypes, particularly trisomy 8. (who.int)

In vitro , overexpression of the mdr-1 gene product, P-glycoprotein (Pgp), in tumor cells can confer high-level resistance to natural product-derived cytotoxics-anthracyclines, vinca alkaloids, epipodophyllotoxins, and taxanes. (cancer.gov)
We tested whether natural killer cells expanded by co-culture with K562 cells transfected with 41BBL and membrane-bound interleukin-15 could kill myeloma cells with a high-risk gene expression profile in vitro and in a unique model which recapitulates human myeloma. (haematologica.org)
In addition to single-agent activity against drug-naive myeloma cell lines in vitro and in vivo, and against main patient-derived cells, TAK-243 overcame resistance to standard and novel medicines. (acusticavisual.net)

that is histopathological y very simi- T cel s, B cel s, natural kil er cel s, LMP1 of EBV can transform ro- lar to that caused by hepatitis B vi- macrophages, and dendritic cells, dent fibroblasts and is expressed rus (HBV) in humans, but it does so and this humanized mouse model in most of the human cancers as- through a different mechanism. (who.int)

The research has shown that HOX B6 is not expressed in hematopoietic stem cells located in the red bone marrow, which are the precursor cells to all types of blood cells, or primordial germ cells (PGCs), the precursor to cells passed on in each generation. (wikipedia.org)
These cells form ecosystems at the primary and at the metastatic site, mutually communicating with one another and with stem cell-generating organs such as the bone marrow. (biomedcentral.com)
Hypoxia stimulates hypoxia inducible factor (HIF)-dependent expression of CXCL12 and KITL promoting mobilization from the bone marrow and recruitment to primary tumor and metastatic sites of CXCR4 + CD11b + bone marrow-derived cells and KITbCD11b + cells assisting vasculogenesis and metastasis respectively (Kuonen et al. (biomedcentral.com)
Interleukin-5 is produced by a number of cell types, and is responsible for the maturation and release of eosinophils in the bone marrow. (biomedcentral.com)
15]. The percentage of bone common of which are trisomy 8, mono- marrow blast cells for estimation of the Myelodysplastic syndrome (MDS) is somy 7 and 5q- [11]. (who.int)
Low-power view of hematoxylin-eosin-stained bone marrow showing hypocellularity, with increased adipose tissue and decreased hematopoietic cells in the marrow space. (medscape.com)
The clinical presentation of patients with aplastic anemia includes signs and symptoms related to the decrease in bone marrow production of hematopoietic cells. (medscape.com)
The theoretical basis for marrow failure includes primary defects in or damage to the stem cell or the marrow microenvironment. (medscape.com)
External insults (eg, infections, radiation, drugs) may disrupt stem cell homeostasis in marrow environment, leading to altered growth. (medscape.com)

Rarely, immunophenotypically immature B-cell precursor acute lymphoblastic leukaemia (BCP-ALL) carries an immunoglobulin-MYC rearrangement (IG-MYC-r). (bvsalud.org)

Viruses are composed of small amounts of DNA or RNA, encapsulated by a protein coat, and may be enclosed in an envelope made of proteins, carbohydrates, and lipids. (biopharminternational.com)
Viruses exploit the enzymes and other host-cell machinery to replicate themselves. (biopharminternational.com)
Contamination of a product with endogenous viruses from cell banks, or adventitious viruses from personnel can have serious clinical implications. (biopharminternational.com)
Viral safety of licensed biological products must be assured by three complementary approaches: (i) thorough testing of the cell line and all raw materials for viral contaminants, (ii) assessing the capacity of downstream processing to clear infectious viruses, and (iii) testing the product at appropriate steps for contaminating viruses. (biopharminternational.com)
Examination of thin sections of Madin Darby canine kidney cells infected with RD114 or avian reticuloendotheliosis virus revealed that these viruses mature from the basolateral membrane domains. (elsevierpure.com)
Because of the availability of DNA and protein sequence data, and of molecularly cloned viruses, these virus systems offer advantages for molecular studies on directional transport of plasma membrane glycoproteins. (elsevierpure.com)
The induction of spongiform myeloencephalopathy by murine leukemia viruses is mediated primarily by infection of central nervous system (CNS) microglia. (omeka.net)
One exception is hu- humanized SCID mice, the use of al oncogenic viruses that are strictly man T-cell lymphotropic virus type 1 surrogate hosts has not proven very species-specific, causing cancer in (HTLV-1): in addition to its ability to useful for defining tumour site con- humans only. (who.int)
These results show that LMP1 , mour viruses classified by IARC in EBV-specific T-cell response. (who.int)

All five mouse Tcl1b and murine Tcl1 mRNAs are abundant in mouse oocytes and two-cell embryos but rare in various adult tissues and lymphoid cell lines. (unife.it)
The immune system is constituted by a very complex network of cells, tissues, and organs that through soluble factors and direct cell-to-cell contacts interact among themselves and with cells belonging to other (organ) systems. (frontiersin.org)
In addition, as the genetic identity of the donor egg from which the ESCs are derived most likely will differ from that of potential recipients, patients who receive ESC-derived cells or tissues may face the same complications that result from organ transplantation (for example, immunorejection, graft-versus-host disease, and need for immunosuppression). (biomedcentral.com)

PR-domain-containing Mds1-Evi1 is critical for long-term hematopoietic stem cell function. (rochester.edu)

A novel gammaretrovirus named xenotropic murine leukemia virus-related virus (XMRV) has been recently identified and found to have a prevalence of 40% in prostate tumor samples from American patients carrying a homozygous R462Q mutation in the RNaseL gene. (biomedcentral.com)
We beforehand reported the era of a transgenic murine mannequin of progeria carrying a human BAC harboring the frequent mutation, G608G, which within the single-copy state develops options of HGPS which might be restricted to the vascular system. (eumorphia.org)

Virus contamination of products derived from human or animal cells can have disastrous clinical consequences causing diseases ranging from common colds and influenza, to acquired immune deficiency syndrome (AIDS), hepatitis, herpes, measles, and poliomyelitis. (biopharminternational.com)
Viral contamination is a risk to all biotechnology products derived from cell lines of human or animal origin. (biopharminternational.com)
Future studies with human pluripotent stem cells may transduce cells towards mesoderm and finally towards cardiac fate. (siu.edu)
Lysates of human chronic myelogenous leukemia cells, which had neither catalytic activity for dihydrofolate reductase nor binding of methotrexate, blocked the inhibiting effect of the antiserum on the function of the enzyme in L1210 cell lysates. (aku.edu)
In double immunodiffusion, these human leukemia cell lysates formed a single precipitin line against the antiserum. (aku.edu)
These findings indicate that nonfunctional dihydrofolate reductase in human leukemia cells share an antigenic determinant(s) with a functional form of the enzyme from L1210 murine leukemia cells. (aku.edu)
Perwaiz Iqbal, M & Rothenberg, SP 1981, ' A nonfunctional protein in human leukemia cells reacts with antiserum to dihydrofolate reductase from L1210 leukemia cells ', Life Sciences , vol. 29, no. 7, pp. 689-696. (aku.edu)
To further elucidate the role of such mutations in leukemia, we will assess their transforming potential in primary murine and human hematopoietic stem cells and in the AML cell line HL60. (uni-muenchen.de)
In this report we present the cloning, mapping, and expression analysis of the human and murine TCL1/Tcl1 locus. (unife.it)
Tcl1b1- Tcl1b5 proteins range from 117 to 123 aa and are 65-80% similar, but they show only a 30-40% similarity to human TCL1b. (unife.it)
We determined the capacity of the leukemia cell line K562 modified to express a membrane-bound form of interleukin (IL)-15 and 41BB ligand (K562-mb15-41BBL) to generate human NK cells with enhanced cytotoxicity. (aacrjournals.org)
Natural killer (NK) cells can kill cancer cells in the absence of prior stimulation and hold considerable potential for cell-based therapies targeting human malignancies ( 1 - 4 ). (aacrjournals.org)
The potential use of embryonic stem cells (ESCs) for cell replacement therapies is limited by ethical concerns and the technical hurdles associated with their isolation from human embryos. (biomedcentral.com)
Experimental Study of Oxaliplatin, cancer spectrum cell screening was found oxaliplatin on a variety of mouse, human tumor cells and effective, including DDP has been resistant cell lines (ovarian cancer Az780, murine leukemia L1Z10, etc.), addition of oxaliplatin on human HT29 colon cancer cell line and its drug-resistant strains of lead is particularly significant inhibition. (cancerlive.net)
The transferred, expanded natural killer cells proliferated in vivo in an interleukin-2 dose-dependent fashion, persisted up to 4 weeks, were readily detectable in the human bone, inhibited myeloma growth and protected bone from myeloma-induced osteolysis. (haematologica.org)
Similar to MLL-AF4 + human ALL, pMIRH-128a - and pMIRH-130b -transplanted mice showed significant leptomeningeal tumor cell infiltration. (ashpublications.org)
Human PD-1 is a homolog of murine PD-1 (mPD-1), which was originally isolated by Ishida et al. (biomedcentral.com)
LINE-1 retrotransposons drive human neuronal transcriptome complexity and functional diversification. (lu.se)
2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)
CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)

Fli-1, a known person in the ETS transcription aspect family members, is also the mark of insertional activation by Friend murine leukemia trojan (F-MuLV) and it is preferentially portrayed in vascular endothelial cells and hematopoietic tissue [6]. (columbiagypsy.net)

Genetic selections in mammalian cell lines have recently been developed for the isolation of mutant cells that are refractory to infection by retroviruses. (biomedcentral.com)
We have investigated the maturation sites of avian and mammalian C-type retroviruses in polarized epithelial cells. (elsevierpure.com)

Transgenic reporter mouse embryonic stem cells were used in this study. (siu.edu)

While PAX5 is an important tumor suppressor gene in B-cell acute lymphoblastic leukemia (B-ALL), it is also involved in oncogenic translocations coding for diverse PAX5 fusion proteins. (bvsalud.org)

Effects of targeted nano-delivery systems combined with hTERT-siRNA and Bmi-1-siRNA on MCF-7 cells. (docksci.com)
Original Article Effects of targeted nano-delivery systems combined with hTERT-siRNA and Bmi-1-siRNA on MCF-7 cells Lei Liu*, Huixiang Li, Min Zhang, Xinquan Lv Department of Pathology, Basic Medical College, Henan Key Laboratory for Tumor Pathology, Zhengzhou University, Henan Province, China. (docksci.com)
Published June 15, 2015 Abstract: The aim of this study was to evaluate the efficiency of a targeted siRNA nano-delivery system to silence the expression of Bmi-1 and hTERT, and to verify the toxicity of this delivery system in MCF-7 breast cancer cells. (docksci.com)
The polyethyleneimine (PEI)/siRNA nano-condensate was synthesized using PEI and modified using an NGR peptide fragment for targeting to tumor cells. (docksci.com)

HIV persistence may arise from ongoing residual virus replication and/or from latently-infected cells defined as the cellular reservoir in which long-lived resting memory CD4+ T cells harbouring an integrated but transcriptionally silent provirus represent the largest pool in the blood (Chomont et al. (europa.eu)
After 72 h, selection media containing 300 ng/mL puromycin was added and cells with antibiotic resistance after 6 days of selection were considered to have been stably infected with virus expressing BMI-1. (cancertools.org)
A single virion is a completely developed virus particle made of 1-50% nucleic acid and 50-99% proteins or glycoproteins and lipids. (biopharminternational.com)
The protocol involves the transformation of pools of resistant cells by cDNA expression libraries, followed by the selection for rare virus-sensitive cells, using multiple rounds of selection after infection by marked viral vector genomes. (biomedcentral.com)
We here report the isolation of two cDNA constructs that each restore virus sensitivity to the R4-7 mutant cell line. (biomedcentral.com)
It's a monkey virus coming out of a monkey cell line and that's the problem, but the spike protein is clearly [causing] the disease. (fromthetrenchesworldreport.com)
Feng et al established in 2008 that approximately 80% of Merkel cell carcinomas in the Northern hemisphere occur after genomic integration by a ubiquitous skin commensal virus, Merkel cell polyoma virus (MCPyV). (medscape.com)
cell for the virus is placed in a mu- studies costly and difficult. (who.int)

See Chronic Leukemias: 4 Cancers to Differentiate , a Critical Images slideshow, to help detect chronic leukemias and determine the specific type present. (medscape.com)

We have recently developed and tested a "second generation" EPOCH regimen (EPOCH II) to replace stem cell transplant for lymphomas requiring high-dose intensity, including poor prognosis untreated aggressive lymphomas, potentially curable relapsed lymphomas, and low-grade lymphomas. (cancer.gov)
1 Significant advances have been made by combining novel agents with autologous peripheral blood stem cell transplantation which allows for long-term disease-free survival in the majority of transplant-eligible patients. (haematologica.org)
Stem cell-like transcriptional reprogramming mediates metastatic resistance to mTOR inhibition. (rochester.edu)
Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)
We discuss these properties with examples both from the hematopoietic and embryonic stem cell (ESC) systems. (lu.se)
The nature of the stem cell substates and their relationship to commitment to differ- entiate and lineage selection can be elucidated in terms of a landscape picture in which stable states can be defined mathematically as attractors. (lu.se)
The essence of MDS is damage of In the current work we examined All our patients were of the high-risk colony-forming units [4], but the defect haematopoietic stem cells of high-risk group and none of them was eligible of the haematopoietic stem cells is not MDS cases for apoptotic and anti-apop- for stem cell transplantation. (who.int)

Earlier KLHL1 antibody studies have shown that knocking-down Fli-1 prospects to designated growth inhibition and death in erythroleukemic cells, indicating a possible use of Fli-1 like a restorative target to induce tumor suppression [18C20]. (columbiagypsy.net)
Bmi-1 can also induce downstream expression of hTERT, which prevents cell aging and results in immortalization. (docksci.com)
Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)

Expression of polycomb protein BMI-1 maintains the plasticity of basal bronchial epithelial cells. (cancertools.org)
Sperm competition risk drives plasticity in seminal fluid proteins that enhance post-copulatory fertilization order yasmin online success. (eviematilda.art)

What pathways are this gene/protein implicaed in? (cancerindex.org)
We are beginning to understand the power of co-inhibitors in the context of lymphocyte homeostasis and the pathogenesis of leukemia, which involves several newly described co-inhibitory pathways, including the programmed death-1 (PD-1) and PD-1 ligand (PD-L1) pathway. (biomedcentral.com)
Even as the nature of the exact cell in which Merkel cell carcinoma oncogenesis occurs is controversial, there is strong support for the notion that Merkel cell carcinoma results from of one of two distinct pathways. (medscape.com)
In terminally differentiated cell fate is coupled to appropriate regulation of the alternative cells, transcriptional networks must be stable and irreversible, pathways. (lu.se)

This domain interacts with adaptor molecules and activates a cascade which regulates the inflammatory status in cells [12]. (fortunejournals.com)

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. (cancerindex.org)
Molecular cloning and transcriptional mapping of the mouse cellular retinoic acid-binding protein gene. (ulaval.ca)

Leukemia, particularly aggressive refractory hematological malignancies unresponsive to upfront therapy, remains a difficult condition to treat. (biomedcentral.com)
Notably, TAK-243 overcame resistance to standard medicines and Rabbit Polyclonal to RNF111 novel providers in cell-line models, including bortezomib and carfilzomib resistance, and showed activity against main cells from relapsed/refractory myeloma individuals. (acusticavisual.net)

In the presence of fibronectin and a small molecule called leukemia inhibitory factor (LIF), which is known to maintain pluripotency and self-renewal, mouse embryonic stem cells responded by exhibiting expressions of both GFP and DsRed. (siu.edu)

In 2006, a major breakthrough was reported in Japan by Takahashi and Yamanaka, who described the generation of induced pluripotent stem cells (iPSCs) from mouse fibroblasts via over expression of defined transcription factors [ 2 ]. (biomedcentral.com)
Other suggested cells of origin include the neural crest‒derived cell of amine precursor uptake and decarboxylation (APUD) system, dermal fibroblasts, pre or pro B cells, residual epidermal stem cells, and epithelial, non-Merkel cell progenitors. (medscape.com)
2008). Historically, this concept is highlighted by the experi- factors are key intrinsic regulators of these fate decisions and mental phenomenon of lineage reprogramming, for example, that fate choice involves modulating networks of transcription by the conversion of fibroblasts to muscles cells following trans- factors. (lu.se)

The encoded protein functions as a sequence-specific transcription factor that is involved in development, including that of lung and skin, and has been localized to both the nucleus and cytoplasm. (wikipedia.org)
These selections have been used to recover lines that block early postentry stages of infection, either before reverse transcription or before nuclear entry. (biomedcentral.com)
Infection of this line was blocked earlier, before the initiation of reverse transcription. (biomedcentral.com)

These mice are devoid of endogenous natural killer and T-cell activity and were used to determine whether adoptively transferred expanded natural killer cells could inhibit myeloma growth and myeloma-associated bone destruction. (haematologica.org)
Multiple myeloma (MM) is a malignant plasma cell disorder with debilitating symptoms related to anemia, immunosuppression, bone destruction, and renal failure. (haematologica.org)

E. Wells containing endothelial cells with microspheres were analyzed for fluorescence. (hsdpathway.com)

An oncoprotein can cause the transformation of a normal cell into a tumor cell. (wikipedia.org)
With the advent of improved immunohistochemical profiling, the tumor was reclassified as Merkel cell carcinoma because shared epithelial and neuroendocrine markers, along with ultrastructural features suggestive of neural crest origin, were observed in both the constituent tumor cell and the physiologic Merkel cell of the skin. (medscape.com)

Identification of binding sites of EVI1 in mammalian cells. (rochester.edu)

To promote adhesion of mouse embryonic stem cells and to initiate integrin-based mechanical signaling, the extracellular matrix protein fibronectin was used. (siu.edu)

however, restoration of CBY1 expression in tumor cells reduces BCSC and its enrichment, thus lnc408 plays an essential role in maintenance of BCSC stemness. (nature.com)
Scope includes mutations and abnormal protein expression. (cancerindex.org)
Comparisons of the gene expression profiles of the expanded NK cells and their unstimulated or IL-2-stimulated counterparts showed marked differences. (aacrjournals.org)
CAR T-cell potency is highly dependent on target antigen expression, and CARs often fail to exert their antitumor activities when antigen expression is low or below a certain threshold. (nature.com)
In an earlier study, Barrett et al used a conditional invertor mouse line for cell-specific expression of an Mll-AF4 fusion protein. (ashpublications.org)
In addition, the low cytotoxicity of T-cells may be related to the high expression level of inhibitory molecules including programmed death-1 (PD-1), LAG-3 and NKG2A in CD8+T cells [ 11 ]. (biomedcentral.com)
In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)
Our results suggest that although trisomy 8 cells are in a pro-apoptotic state, they are checked by the enhanced expression of anti-apoptotic signals which provide them with their proliferative advantage. (who.int)

Recruitment of CD11b + CD11c + myelomonocytic cells to the metastatic site was also found after whole thorax irradiation at a dose of 15 Gy of mice that significantly enhanced seeding and metastatic growth of intravenously injected cancer cells. (biomedcentral.com)

IL-2 treatment acutely increased the CD4 cells, all within the CD4RO subset, and increased NK and LAK cells. (cancer.gov)
E3 ubiquitin ligases such as cereblon20 and murine double cIAP1 Ligand-Linker Conjugates 11 Hydrochloride minute 2 (MDM-2),21-24 which ubiquitinate a small subset of client proteins and could provide greater target specificity, represent one example. (acusticavisual.net)

We investigated the hypothesis that neural stem cells (NSCs) possess an intrinsic capacity to 'rescue' dysfunctional neurons in the brains of aged mice. (omeka.net)
Neural stem cells as engraftable packaging lines can mediate gene delivery to microglia: evidence from studying retroviral env-related neurodegeneration. (omeka.net)
Neural stem cells as tools for understanding retroviral neuropathogenesis. (omeka.net)
[ 2 ] As neuroendocrine carcinomas were thought to be of neural crest origin, in 1978 Tang and Toker proposed that this carcinoma might be derived from Merkel cells. (medscape.com)
Merkel cells were thought to be of neural crest origin because they are excitable cells that secrete neuropeptides and stain positively for neuroendocrine markers. (medscape.com)

Transplantation of Mll-AF4 + LSK cells virally expressing miR-128a ( pMIRH-128a ) or miR-130b ( pMIRH-130b ) induced a hematologic malignancy in mice with increasing penetrance upon propagation into secondary and tertiary recipients. (ashpublications.org)

P19ARF can prevent the inactivation of p53, which induces cell growth arrest, senescence and apoptosis. (docksci.com)
This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. (cancerindex.org)
Low levels of pyruvate induced by a positive feedback loop protects cholangiocarcinoma cells from apoptosis. (cancerindex.org)

In the present review, we describe and discuss the effects of extremely low frequency (ELF)-EMF and pulsed EMF on cell signals and factors relevant to the activation of danger signals and innate immunity cells. (frontiersin.org)
In innate immunity, immune cells are activated by their pattern recognition receptors (PRRs), which bind to pattern molecules of invading pathogens. (fortunejournals.com)

These data suggest a similar or complementary function of these proteins in early embryogenesis. (unife.it)

The reporter cell line shows the cellular endogenous activity of pluripotency gene Oct3/4 with green fluorescent protein (GFP) while also reporting mesoderm-specific gene, Brachyury, activity with DsRed fluorescent protein. (siu.edu)
Here, we studied the oncogenic function of the PAX5-JAK2 fusion protein in a mouse model expressing it from the endogenous Pax5 locus, resulting in inactivation of one of the two Pax5 alleles. (bvsalud.org)

Anatomy6

Organisms10

Diseases17

Chemicals and Drugs8

Analytical, Diagnostic and Therapeutic Techniques and Equipment3

Phenomena and Processes9

Information Science2

Moloney murine leukemia1

Marked Moloney murine leuke1

Acute myeloid5

Lymphoma6

Progenitor8

Myeloid3

Reverse transcriptase1

Kinase6

Recombinant4

Genes16

Encodes3

Fetal3

Differentiation13

MRNA5

Receptor7

Mammary3

Tumors11

Proliferation7

Vitro3

Dendritic cells1

Marrow9

Immunoglobulin1

Viruses9

Tissues3

Hematopoietic stem1

Mutation2

Human20

Insertional1

Retroviruses2

Transgenic1

Translocations1

SiRNA4

Virus8

Cancers1

Stem cell7

Induce3

Plasticity2

Pathways4

Regulates1

Transcriptional2

Refractory2

Inhibitory factor1

Fibroblasts3

Transcription3

Bone2

Endothelial1

Tumor cell2

Mammalian1

Embryonic stem1

Expression8

Myelomonocytic1

Subset2

Neural5

Virally1

Apoptosis3

Innate immunity2

Embryogenesis1

Endogenous2