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  • phosphorylation
  • Attwood PV, Ludwig K, Bergander K, Besant PG, Adina-Zada A, Krieglstein J, Klumpp S (2010) Chemical phosphorylation of histidine-containing peptides based on the sequence of histone H4 and their dephosphorylation by protein histidine phosphatase. (springer.com)
  • signal
  • Abraham SJ, Kobayashi T, Solaro RJ, Gaponenko V (2009) Differences in lysine pKa values may be used to improve NMR signal dispersion in reductively methylated proteins. (springer.com)
  • specific
  • In turn, these modifications induce highly specific changes in the chemical environments of individual protein residues, which are readily detected by high-resolution NMR spectroscopy. (springer.com)
  • acetylation
  • Our data confirms substantial changes in the antioxidant network, glycolysis and electron transport, and indicates links between protein acetylation and Mtz resistance, including cross-resistance to deacetylase inhibitor Trichostatin A (TSA) in Mtz resistant lines. (gigadb.org)
  • Our data demonstrate understanding of Mtz resistance must be broadened to post-transcriptional and post-translational responses, and that Mtz resistance is polygenic, driven by isolate-dependent variation, and is correlated with changes in protein acetylation networks. (gigadb.org)
  • kinases
  • Here we focus on three important signaling and regulatory IDR-containing protein families in humans, namely G-protein coupled receptors (GPCRs), which are transmembrane signaling proteins, the nuclear factors of activated T-cells (NFATs), which are transcription factors (TFs), and the Src family kinases (SFKs), which are signaling enzymes. (jbsdonline.com)
  • enzymes
  • We provide an overview of studies focusing on the origin and evolution of regulatory enzymes as well as the evolutionary dynamics of modification sites. (embopress.org)
  • We have developed a series of activity-based probes to investigate the functions and substrate-scope of enzymes associated with the prokaryotic ubiquitin-like protein (Pup). (washington.edu)
  • peptide
  • In this approach, protein identification is achieved after protein separation by one- or two-dimensional (1D or 2D) polyacrylamide gel electrophoresis followed by protein digestion usually with trypsin, analyses of the resulting peptide mixture using various MS approaches, and data base search. (mcponline.org)
  • The identification and localization of the modification can then be obtained using MS/MS analyses of the peptide bearing the modification after proteolysis of the protein. (mcponline.org)
  • Proteins square measure synthesized by ribosomes translating ribonucleic acid into peptide chains, which can then endure PTM to make the mature supramolecular product. (insightconferences.com)
  • In this study, with synthetic biology strategy, recombinant mussel plaque protein with a zwitterionic peptide inspired by molecular chaperone was engineered through post-translational modification, in which 3,4-dihydroxyphenylalanine was residue-specifically obtained efficiently from tyrosine with tyrosinase coexpressed in vivo. (usda.gov)
  • Nucleotide
  • The much longer splice variant, caused by a deletion of exons 9 and 10, does not have the proteins coating the nucleotide binding pocket aswell as the next Ca2+ binding site. (biomasswars.com)
  • Differential
  • The overall impact of FAP activity on the protein composition of the MEF secretomes was investigated using differential metabolic labelling coupled with quantitative proteomic analysis. (edu.au)
  • proteome
  • Post-translationally modified proteins make up the majority of the proteome and establish, to a large part, the impressive level of functional diversity in higher, multi-cellular organisms. (springer.com)
  • Protein turnover is essential for the vitality of a functional proteome, as it allows for old and damaged proteins to be destroyed and replaced with newly synthesized copies. (escholarship.org)
  • degradomics
  • Terminal Amine Isotopic Labelling of Substrates (TAILS) based degradomics identified endopeptidase cleavage sites in ECM and associated proteins, including type 1 collagen, as well as dipeptidyl peptidase cleavage of C1qT6, which was confirmed in vitro. (edu.au)
  • biochemical
  • The complex signaling pathways and biochemical processes in our cells arise from a relatively small number (~21,000) of protein-coding genes. (washington.edu)
  • Cell
  • The rational designed chimeric protein coating in this work could successfully anchor to various substrates and exhibit excellent antifouling performance in resisting protein adsorption, cell attachment, and bacterial adhesion with eminent biocompatibility. (usda.gov)
  • function
  • This polyglutamine tract is thought to confer a toxic gain of function to the bearing proteins, which leads to late onset and progressive loss of neurons in specific regions of the central nervous system. (unitn.it)
  • Studies on young animal eye lenses reveal how the degree of these modifications change as a function of protein solubility and shows that specific structural motifs and amino acid repeats serve as isomerization hotspots. (escholarship.org)
  • nuclear
  • HO-1 is reportedly induced by various stresses and by multiple transcription factors, e.g., heat shock factor 1 (HSF1), nuclear factor-κB (NF-κB), activator protein-1 (AP-1), and nuclear factor-erythroid 2-related factor 2 (Nrf2) (Fig. 2) . (nih.gov)