Protein microtubule nucleation. Medical search. Frequent questions

The γ-TuRC is typically found as the core functional unit in a microtubule organizing center (MTOC), such as the centrosome in some animal cells or the spindle pole bodies in fungi and algae. (wikipedia.org)
The γ-TuRCs in the centrosome nucleate an array of microtubules in interphase, which extend their (+)-ends radially outwards into the cytoplasm towards the periphery of the cell. (wikipedia.org)
Other cells, such as neurons, skeletal muscle cells, and epithelial cells, which do have MTOCs, possess arrays of microtubules not associated with a centrosome. (wikipedia.org)
Five proteins in Drosophila (DGT2 through DGT6) have been identified that are necessary and responsible for facilitating the localization of γ-tubulin to existing MTs and are not associated with its localization at the centrosome. (wikipedia.org)
Conversely, we identify a novel role for two microtubule-growth and nucleation agents, Ran and CLASP, in the establishment of the centrosome-independent force during anaphase.Their involvement raises the interesting possibility that microtubule polymerization of midzone microtubules is continuously required to sustain chromosome segregation during mitosis. (ens-lyon.fr)
The generation of cellular microtubules is initiated at specific sites such as the centrosome and the Golgi apparatus that contain nucleation complexes rich in γ-tubulin. (crcm-marseille.fr)
Myomegalin was identified as a centrosome/Golgi protein associated with cyclic nucleotide phosphodiesterase. (crcm-marseille.fr)
Depletion of Myomegalin by small interfering RNA delays microtubule growth from the centrosome and Golgi apparatus, and decreases directional migration of RPE1 cells. (crcm-marseille.fr)
In contrast to well-studied fungal and animal cells, plant cells assemble bipolar spindles that exhibit a great deal of plasticity in the absence of structurally defined microtubule-organizing centers like the centrosome. (nsf.gov)
Although many key factors contributing to MT nucleation and organization are well conserved in different kingdoms, the centrosome, representing the most prominent microtubule organizing centers ( MTOC s), disappeared during plant evolution as angiosperms lack the structure. (nsf.gov)
Acts as a centrosome maturation factor, probably by maintaining the integrity of the pericentriolar material and proper microtubule nucleation at mitotic spindle poles. (univ-amu.fr)
Importantly, while previous investigations have focused attention on AKAP350 at the centrosome and the Golgi apparatus, our recent studies have led to the recognition that in most cells a large cytosolic pool of AKAP350 associates with CCAR1 and caprin and regulates mRNA trafficking and participates in microtubule-dependent stress granule formation. (grantome.com)
They develop in a juxtanuclear position, adjacent to the centrosome, the microtubule organizing centre of the cell, and share some protein components. (biorxiv.org)
Despite the long-standing observation that aggresomes/Lewy bodies and the centrosome sit side-by-side in the cell, no studies have been done to see whether these protein accumulations impede the organelle function. (biorxiv.org)
We investigated whether the formation of aggresomes affected key centrosome functions: its ability to organize the microtubule network and to promote cilia formation. (biorxiv.org)
The juxtanuclear location of the aggresome is shared by the centrosome, the microtubule organising centre of the cell. (biorxiv.org)
Within the cell, the arrangement of microtubules is controlled by a variety of discreet microtubule-organizing centers (MTOCs), but in most cells, the dominant MTOC is the centrosome, a bipartite structure consisting of a central pair of barrel-shaped centrioles and a surrounding matrix of pericentriolar material (PCM). (nih.gov)
Second, we seek to understand how centrosome size and microtubule-organizing activity are controlled. (nih.gov)
It specifically recognizes and cuts polyglutamylated microtubules with short polyglutamate tails to promote reorganization of cellular microtubule arrays and the release of microtubules from the CENTROSOME following nucleation. (bvsalud.org)

During mitosis, chromosomes are connected to a microtubule-based spindle. (ens-lyon.fr)
Using a specialized microscope equipped with a time-lapse camera to image fluorescence-tagged proteins in real-time, an NIH-funded team has captured a critical step in the process of cell division, or mitosis: how filaments called microtubules (red) form new branches (green) and fan out to form mitotic spindles. (nih.gov)
Franck Perez interconnects Secretory protein, Mitosis, Microcephaly and Streptavidin in the investigation of issues within Golgi apparatus. (research.com)
Moreover, the implication that the active form of Ran is concentrated in the vicinity of chromosomes suggests that these findings may resolve the long-standing question of how chromosomes can influence spindle assembly in the absence of microtubule-nucleating organelles (i.e., as in plant mitosis or mammalian meiosis). (silverchair.com)
Acentrosomal asters are small populations of microtubules that are nucleated on or around the condensed chromosomes at the beginning of mitosis. (radikes.com)
These experiments revealed a possible novel role for motor proteins during mitosis. (radikes.com)
An interesting characteristic of the cmm phenotype is the presence of regions of cells that are unable to complete mitosis because no microtubules exist to connect chromatin and centrosomes. (nordicmubio.com)
Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, proliferation, apoptosis, mitosis, and in vesicle-mediated transport processes (PubMed:11896197, PubMed:30290153). (icr.ac.uk)

Through use of TIRF microscopy, researchers have visually observed the nucleation of branching microtubules in Drosophila cells during the formation of the mitotic spindle. (wikipedia.org)
Besides sliding apart antiparallel microtubules during spindle elongation, the mitotic kinesin-5, Eg5, promotes microtubule polymerization, emphasizing its importance in mitotic spindle length control. (elsevierpure.com)
It is predicted that MCPH gene mutations may lead to the disease phenotype due to a disturbed mitotic spindle orientation, premature chromosomal condensation, signalling response as a result of damaged DNA, microtubule dynamics, transcriptional control or a few other hidden centrosomal mechanisms that can regulate the number of neurons produced by neuronal precursor cells. (biomedcentral.com)

Regulates centrosomal microtubule nucleation. (helixlabs.ai)
Along with GIT1, positively regulates microtubule nucleation during interphase (PubMed:27012601). (icr.ac.uk)
Finally, CITK regulates both astral-MT nucleation and stability. (ox.ac.uk)
This protein also promotes the nucleation and elongation of new actin filaments and regulates cell growth through the stabilization of microtubules. (utsouthwestern.edu)

Franck Perez works mostly in the field of Microtubule nucleation, limiting it down to concerns involving Nocodazole and, occasionally, Golgi organization, Organelle and Subcellular localization. (research.com)
Recent findings by several groups have shown that the GTP-bound form of Ran is necessary for the nucleation and organization of microtubule structures during M phase. (silverchair.com)
I was interested in determining if the mitotic kinesins and dynein played a role in nucleation, stabilization and/or organization of the acentrosomal asters. (radikes.com)
I found motors that seemed to affect microtubule nucleation or stabilization, microtubule polymerization, and microtubule organization. (radikes.com)
Phosphorylates the tubulin chaperone TBCB and thereby plays a role in the regulation of microtubule biogenesis and organization of the tubulin cytoskeleton. (icr.ac.uk)
ASPM and CITK affect the organization of astral microtubules (MT), and low doses of MT-stabilizing drug revert the spindle orientation phenotype produced by their knockdown. (ox.ac.uk)
Effects of nickel on microtubule assembly and the possible mechanisms of nickel -induced perturbation in microtubule organization. (cdc.gov)

In this particular experimental system, the team led by Sabine Petry at Princeton University, Princeton, NJ, studies the dynamics of microtubules in a cell-free extract of cytoplasm taken from the egg of an African clawed frog ( Xenopus laevis ). (nih.gov)
Instead, Ran and its associated proteins shift their focus from nuclear transport to the regulation of microtubule dynamics. (silverchair.com)
An Excel sheet containing numerical data for the quantification of microtubule nucleation and microtubule dynamics presented in Figure 1 and Figure 1-figure supplement 2 . (elifesciences.org)
SSNA1 modulates all parameters of microtubule dynamics. (elifesciences.org)
One implication is that regulation of Eg5 motile properties by regulatory proteins or small molecule inhibitors could also have effects on intracellular microtubule dynamics. (elsevierpure.com)
In the lab of Dr. Isabelle Vernos at the Center for Genomic Regulation in Barcelona, Spain I investigated microtubule dynamics during cell division. (radikes.com)
In addition to this cell work I learned how to harvest Xenopus laevis extracts, how to deplete key mitotic regulators from the extract and how to induce the formation of spindles in these extracts, in order to probe how key mitotic factors regulate microtubules dynamics and spindle formation. (radikes.com)
Here, we report that AQP5 promotes microtubule assembly and helps maintain the assembled microtubule steady state levels with slower turnover dynamics in cells . (plos.org)
These findings indicate that AQP5-mediated regulation of microtubule dynamics modulates airway epithelial barrier properties and epithelial function. (plos.org)
ASPM and CITK regulate spindle orientation by affecting the dynamics of astral microtubules. (ox.ac.uk)

We demonstrate that a Drosophila Golgi protein, Gorab, is present not only in the trans-Golgi but also in the centriole cartwheel where, complexed to Sas6, it is required for centriole duplication. (uni-koeln.de)
One isoform, CM-MMG, harbors a conserved domain (CM1), recently described as a nucleation activator, and is related to a family of γ-tubulin binding proteins, which includes Drosophila centrosomin. (crcm-marseille.fr)
The expression of PP4C was reduced to 25% of the normal protein level in a mutant strain of Drosophila termed centrosomes minus microtubules (cmm). (nordicmubio.com)

This phenotype implicates PP4C in the regulation of the nucleation and/or stabilization of microtubules. (nordicmubio.com)

In the meiosis of many species, the process of chromosome segregation requires that bipolar spindles be formed without the aid of dedicated microtubule organizing centers, such as centrosomes. (nsf.gov)
A decrease in the critical concentration of MT protein necessary for initiation of MT assembly and an increase in the number of effective initiation centers in the presence of nickel as examined by electron microscopy confirmed the finding that nickel exerts its effect at the initial nucleation phase. (cdc.gov)

The early cells of the pre-implantation mouse embryo utilise a unique non-centrosomal MTOC, in the form of an interphase microtubule bridge joining sister cells. (wikipedia.org)

It recruits γ-tubulin nucleating complexes and promotes microtubule nucleation. (crcm-marseille.fr)
Human SSNA1 promotes microtubule nucleation and suppresses microtubule dynamicity. (elifesciences.org)
Thus, we propose a microtubule polymerase mechanism in which Eg5 at the plus-end promotes a curved-to-straight transition in tubulin that enhances lateral bond formation and thereby promotes microtubule growth and stability. (elsevierpure.com)
propose a model in which Eg5 binding to tubulin promotes a curved-to-straight transition that drives microtubule assembly. (elsevierpure.com)

Here we establish optical control of intracellular transport by using light-sensitive heterodimerization to recruit specific cytoskeletal motor proteins (kinesin, dynein or myosin) to selected cargoes. (nature.com)
Cytoskeletal microtubules ( MT s) have a multitude of functions including intracellular distribution of molecules and organelles, cell morphogenesis, as well as segregation of the genetic material and separation of the cytoplasm during cell division among eukaryotic organisms. (nsf.gov)

These complexes appear to coordinate the movement intracellular proteins and messenger RNAs responsible for far ranging processes within cells. (grantome.com)

In the cortical array of plants, as well as in the axons of neurons, it is theorised that microtubules nucleate from existing microtubules via the action of severing enzymes such as katanin. (wikipedia.org)
Ori-McKenney, K. M., Jan, L. Y. & Jan, Y. N. Golgi outposts shape dendrite morphology by functioning as sites of acentrosomal microtubule nucleation in neurons. (nature.com)

Our findings suggest that during animal evolution, a Golgi protein has arisen with a second, apparently independent, role in centriole duplication. (uni-koeln.de)
Myomegalin is necessary for the formation of centrosomal and Golgi-derived microtubules. (crcm-marseille.fr)
It localizes at the cis-Golgi and can localize to microtubule plus-ends. (crcm-marseille.fr)
In the field of Cell biology, his study on Golgi apparatus and Transport protein overlaps with subjects such as Palmitoylation. (research.com)
Franck Perez focuses on Cell biology, Golgi apparatus, Endoplasmic reticulum, Secretory pathway and Microtubule. (research.com)
His primary areas of investigation include Cell biology, Golgi apparatus, Endoplasmic reticulum, Secretory pathway and Microtubule. (research.com)
golgi reassembly stacking protein 1 [Sourc. (gsea-msigdb.org)
AKAP350 is localized to both centrosomes and the Golgi apparatus, and we have demonstrated that depletion of AKAP350A with siRNA leads to disruption of the Golgi structure as well as alteration of polymerizing microtubules. (grantome.com)
First, we will determine the role of AKAP350A and its associated proteins in regulating the structure and function of the Golgi apparatus. (grantome.com)

These filaments of the cytoskeleton typically form through polymerization of α- and β-tubulin dimers, the basic building blocks of the microtubule, which initially interact to nucleate a seed from which the filament elongates. (wikipedia.org)
Theskeleton that supports the structure of our cells, termed cytoskeleton is formed of several kinds of polymers including actin and microtubules. (dresdencondensates.org)
The authors identified a protein associated to the cytoskeleton, named Tau, that is able to phase-separate from the cytosol. (dresdencondensates.org)
His study focuses on the intersection of Microtubule and fields such as Microtubule organizing center with connections in the field of Detyrosination and Cytoskeleton. (research.com)
Cell growth, division, and motility are highly dependent upon an organized and dynamic microtubule cytoskeleton. (nih.gov)
Cell motility, adhesion, cytokinesis, and other functions of the cell cortex are mediated by reorganization of the actin cytoskeleton and several formin homology (FH) proteins have been associated with these processes. (utsouthwestern.edu)

In conclusion, the Myomegalin gene encodes different isoforms that regulate microtubules. (crcm-marseille.fr)

Among its other functions, this radial array is used by microtubule-based motor proteins to transport various cargoes, such as vesicles, to the plasma membrane. (wikipedia.org)

E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. (helixlabs.ai)
Here, we identified a novel PP4 inhibitor, protein phosphatase 4 inhibitory protein (PP4IP) and elucidated its cellular functions. (molcells.org)
Aggresomes are closely-related cellular accumulations of misfolded proteins. (biorxiv.org)

EB-MMG binds EB1 and affects its loading on microtubules and microtubule growth. (crcm-marseille.fr)
Together, these proteins comprise an enzymatic cycle by which Ran binds GTP, hydrolyzes it to GDP (due to the activity of Ran-GAP1), releases the GDP (due to RCC1 activity), and rebinds GTP (due to the presence of a relatively high GTP concentration in the cell). (silverchair.com)
Eg5 binds preferentially to microtubules over free tubulin, which contrasts with microtubule-depolymerizing kinesins that preferentially bind free tubulin over microtubules. (elsevierpure.com)
Binds to the caspase-cleaved p110 isoform of CDC2L1 and CDC2L2, p110C, but not the full-length proteins (PubMed:12624090). (icr.ac.uk)
An AAA ATPase that binds and severs MICROTUBULES. (bvsalud.org)

These branching microtubules maintain the same polarity as their mother microtubules, and their assembly involves the binding of non-centrosomal γ-TuRCs to the sides of existing microtubules through the augmin complex. (wikipedia.org)
MT ‐dependent MT nucleation is particularly noticeable in plant cells because it accounts for the primary source of MT generation for assembling spindle, phragmoplast, and cortical arrays when the γ‐tubulin ring complex is anchored and activated by the augmin complex. (nsf.gov)

protein regulator of cytokinesis 1 [Source. (gsea-msigdb.org)

While we and others have demonstrated that AKAP350 can potentially scaffold a wide range of proteins, the actual composition of scaffolded complexes is likely both cell specific as well as subcellular organelle specific. (grantome.com)
Small angle neutron scattering (SANS) provides unique insight into biomacromolecular complexes by combining solvent contrast variation (H2O:D2O exchange) with either natural contrast between different classes of biomolecules (proteins, RNA/DNA, lipids/detergents) and/or by applying artificial contrast, i.e. deuteration of specific biomolecules. (lu.se)
In a first couple of examples, I will show how distance and shape restraints from SANS have helped to improve the uniqueness of structural models for two multi-protein-RNA complexes, in combination with NMR restraints and building blocks from crystallography [1, 2]. (lu.se)

New microtubules are not nucleated and extended, and the cells fail to respond to polarity cues. (biorxiv.org)
The protein encoded by this gene contains two FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. (utsouthwestern.edu)

This gene encodes a protein that has been shown to function as a guanine nucleotide release factor in mouse and to regulate the expression and function of the Nav1.5 cardiac sodium channel in human. (nih.gov)
In humans, the CRYAA gene encodes a 173 amino acid residue protein by single copy genes located on chromosome 21. (molvis.org)

These non-centrosomal microtubule arrays can take on various geometries-such as those leading to the long, slender shape of myotubes, the fine protrusions of an axon, or the strongly polarized domains of an epithelial cell. (wikipedia.org)
Chloroquine inhibits human retina pigmented epithelial cell growth and microtubule nucleation by downregulating p150 glued . (bvsalud.org)

Some cells however, such as those of higher plants and oocytes, lack distinct MTOCs and microtubules are nucleated via a non-centrosomal pathway. (wikipedia.org)

The microtubules and actin filaments within the phragmoplast serve to guide vesicles with cell wall material to the growing cell plate. (sciforums.com)

In vivo, cells get around this kinetic barrier by using various proteins to aid microtubule nucleation. (wikipedia.org)
CLIP-170 highlights growing microtubule ends in vivo. (research.com)
[ 6 ] In humans, about 23 different unrelated proteins are known to form amyloid fibrils in vivo. (medscape.com)

TPX2 microtubule nucleation factor [Source. (gsea-msigdb.org)

This complex, with its 13-fold symmetry, acts as a scaffold or template for α/β tubulin dimers during the nucleation process-speeding up the assembly of the ring of 13 protofilaments that make up the growing microtubule. (wikipedia.org)
Thesis Title : Study of Microtubule Assembly by some Microtubule Nucleation Promoters. (rgcb.res.in)
A beta-hairpin comprising the nuclear localization sequence sustains the self-associated states of nucleosome assembly protein 1. (colorado.edu)
While plants employ some evolutionarily conserved proteins to regulate spindle morphogenesis and remodeling, many essential spindle assembly factors found in vertebrates are either missing or not required for producing the plant bipolar microtubule array. (nsf.gov)
Plants also produce proteins distantly related to their fungal and animal counterparts to regulate critical events such as the spindle assembly checkpoint. (nsf.gov)
Plant spindle assembly initiates with microtubule nucleation on the nuclear envelope followed by bipolarization into the prophase spindle. (nsf.gov)
Uncovering mitotic functions of novel proteins for spindle assembly in plants will illuminate both common and divergent mechanisms employed by different eukaryotic organisms to segregate genetic materials. (nsf.gov)
In contrast, overexpression of AQP5 increased assembly of microtubules, with evidence of increased MT stability, and promoted the formation of long straight microtubules in the apical domain of the epithelial cells. (plos.org)
The protein encoded by this gene is thought to function as a scaffolding protein for the Wnt-induced assembly of a disheveled (Dvl)-Rho complex. (utsouthwestern.edu)
The combination of a shortened lag time, an increase in the initial rate and the final extent of MT assembly indicates that nickel may exert its effect at the initial nucleation phase. (cdc.gov)

Mitotic kinesin-5 (Eg5) motors promote microtubule polymerization through an unknown mechanism. (elsevierpure.com)
To understand the mechanism of the nickel (7440020) induced change in microtubules (MTs), the effect of nickel at 0.01 to 3.0 millimolar (mM) concentrations was investigated on the kinetic parameters of in-vitro MT polymerization. (cdc.gov)

This cap provides both stability and protection to the microtubule (-) end from enzymes that could lead to its depolymerization, while also inhibiting (-) end growth. (wikipedia.org)

His Microtubule study integrates concerns from other disciplines, such as Focal adhesion, Exocytosis, Secretion assay, Secretion and Cell membrane. (research.com)
A ) Representative images of a templated microtubule nucleation assay in which microtubule extensions (gray) were nucleated from GMPCPP-stabilized seeds (red) in the presence and absence of SSNA1. (elifesciences.org)
BK001, the actin binding protein spin-down assay kit provides G- or F-actin (rabbit skeletal muscle origin) plus positive (α-actinin ) and negative (Bovine Serum Albumin, BSA) binding control proteins. (tebubio.com)
Using a microtubule regrowth assay, I observed the nucleation and formation of acentrosomal asters through immunofluorescence and confocal time-lapse imaging. (radikes.com)

In cell biology, microtubule nucleation is the event that initiates de novo formation of microtubules (MTs). (wikipedia.org)

protein phosphatase 1 catalytic subunit be. (gsea-msigdb.org)
protein phosphatase 2 catalytic subunit al. (gsea-msigdb.org)
Protein serine/threonine phosphatase 4R1 is a protein which is associated with the catalytic subunit of protein serine/threonine phosphatase 4 (PP4C) and may possibly regulate the activity of the enzyme. (nordicmubio.com)

Polymorphisms that slightly vary native peptides or inflammatory processes set the stage for abnormal protein folding and amyloid fibril deposition. (medscape.com)

A kinase anchoring proteins (AKAPs) compromise the largest group of multifunctional scaffolding proteins, which anchor not only Type II cAMP- dependent protein kinase (PKA), but also a variety of protein kinases, phosphatases, phosphodiesterases and targets of second messenger regulated signaling. (grantome.com)
AKAP350/450 splice variants potentially scaffold PKA, protein kinase C? (grantome.com)
PKN, casein kinase 1, phosphodiesterase 4D3, protein phosphatases 1 and 2a and calmodulin as well as a number of putative downstream effectors. (grantome.com)
This kinase is recruited to the mother centriole by its receptor, a coiled-coil protein known as SPD-2. (nih.gov)
In this report, we show that ASPM may control spindle positioning by interacting with citron kinase (CITK), a protein whose loss is also responsible for severe microcephaly in mammals. (ox.ac.uk)

Tau concentrates the tubulin dimers, which in turn polymerize to make microtubules inside the tau droplet. (dresdencondensates.org)

Since the nucleation and anchoring of microtubules is executed by the PCM, the microtubule-organizing capacity of centrosomes can be adjusted by controlling PCM levels. (nih.gov)

A HuProt microarray composed of 17,225 human full-length proteins with N-terminal glutathione S-transferase (GST) tags was used to identify protein-protein interactions. (molvis.org)
One-hundred and twenty-seven of 17,225 human full-length proteins were identified that interact with CRYAA. (molvis.org)

Protein aggregates are the pathogenic hallmarks of many different neurodegenerative diseases and include the Lewy bodies found in Parkinson's disease. (biorxiv.org)
Amyloidosis results from the accumulation of pathogenic amyloids-most of which are aggregates of misfolded proteins-in a variety of tissues. (medscape.com)

Further, we review the corresponding genes and the proteins encoded by these genes, their possible role in the developing brain and reported mutations of these genes. (biomedcentral.com)
Many mechanisms of protein function contribute to amyloidogenesis, including "nonphysiologic proteolysis, defective or absent physiologic proteolysis, mutations involving changes in thermodynamic or kinetic properties, and pathways that are yet to be defined. (medscape.com)

Using mutant and RNAi analysis, we show that the microtubule-bundling protein SPD-1/MAP-65 and BMK-1/kinesin-5 act as a brake opposing the force generated by the spindle midzone. (ens-lyon.fr)

Franck Perez mainly focuses on Cell biology, Microtubule, Tubulin, Microtubule nucleation and Astral microtubules. (research.com)
His studies deal with areas such as Astral microtubules and Microtubule nucleation as well as Microtubule. (research.com)

3.0: hematopoietic cell-specific Lyn substrate 1 (HCLS1), Kelch domain-containing 6 (KLHDC6), sarcoglycan delta (SGCD), KIAA1706 protein (KIAA1706), RNA guanylyltransferase and 5′-phosphatase (RNGTT), chromosome 10 open reading frame 57 (C10orf57), chromosome 9 open reading frame 52 (C9orf52), and plasminogen activator, urokinase receptor (PLAUR). (molvis.org)
protein phosphatase 1 regulatory subunit 1. (gsea-msigdb.org)
protein phosphatase 2 scaffold subunit Aal. (gsea-msigdb.org)
Protein phosphatase 4 (PP4) is a crucial protein complex that plays an important role in DNA damage response (DDR), including DNA repair, cell cycle arrest and apoptosis. (molcells.org)
Antibody raised against synthetic peptide corresponding to amino acids 38 to 52 of the human protein phosphatase 4R1. (nordicmubio.com)
Detects protein phosphatase X/A (PP4 R1) protein by Western blot (5-10 µg/ml). (nordicmubio.com)
Purification and identification of a novel subunit of protein serine/threonine phosphatase 4. (nordicmubio.com)
In particular, one factor we have recently discovered is protein phosphatase 1 (PP1). (nih.gov)

At least two of these have different roles, demonstrating a previously unknown mechanism to control microtubules in vertebrate cells. (crcm-marseille.fr)

Septin 7 is a centrosomal protein that ensures S phase entry and microtubule nucleation by maintaining the abundance of p150 glued . (bvsalud.org)
kizuna centrosomal protein [Source:HGNC Sy. (gsea-msigdb.org)

CRYAA interacted with these proteins to maintain their solubility and decrease the accumulation of denatured target proteins. (molvis.org)
Thus, the dephosphorylation of target proteins by PP4 is equally important in DDR as much as phosphorylation by kinases. (molcells.org)

Of note, compared to fungal and animal systems, relatively little is known about how plant cells remodel the spindle microtubule array during anaphase. (nsf.gov)

Functional annotation clustering showed that they belong to cell cycle, organelle or nuclear lumen, protein transport, and DNA binding and repair clusters. (molvis.org)
During the G1, S, and G2 phases of the cell cycle, the nucleocytoplasmic transport machinery must tirelessly zigzag between the nucleus and cytoplasm while directing other proteins to the correct subcellular compartment. (silverchair.com)

We find that when aggresomes are present, neuronal cells are unable to organise their microtubule network. (biorxiv.org)

The γ-tubulin combines with several other associated proteins to form a conical structure known as the γ-tubulin ring complex (γ-TuRC). (wikipedia.org)
adaptor related protein complex 2 su. (gsea-msigdb.org)

Studies using Xenopus egg extracts have identified a novel form of microtubule nucleation that generates fan-like branching arrays, in which new microtubules grow at an angle off of older microtubules. (wikipedia.org)

Our results provide a functional link between two established microcephaly proteins. (ox.ac.uk)

Anatomy42

Organisms20

Diseases2

Chemicals and Drugs100

Analytical, Diagnostic and Therapeutic Techniques and Equipment30

Phenomena and Processes61

Disciplines and Occupations1

Technology, Industry, Agriculture3

Information Science6

Health Care3

Centrosome19

Mitosis8

Mitotic spindle3

Regulates4

Organization7

Dynamics10

Drosophila3

Stabilization1

Centers2

Interphase microtubule1

Promotes4

Cytoskeletal2

Intracellular1

Neurons2

Golgi9

Cytoskeleton6

Regulate microtubules1

Motor proteins1

Cellular3

Binds5

Augmin complex2

Cytokinesis1

COMPLEXES3

Polarity2

Encodes2

Epithelial cell2

MTOCs1

Actin filaments1

Vivo3

TPX21

Assembly10

Polymerization2

Stability1

Assay4

Initiates1

Catalytic subunit3

Peptides1

Kinase5

Tubulin dimers1

Centrosomes1

Full-length proteins2

Aggregates2

MUTATIONS2

Kinesin1

Astral2

Phosphatase8

Vertebrate1

Centrosomal protein2

Target proteins2

Anaphase1

Cell cycle2

Neuronal1

Complex2

Identified a novel1

Microcephaly1