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  • Vivo
  • The noninvasive 9L glioma cell was transfected with either full-length BEHAB/brevican or the HABD and tested for invasion in in vitro and in vivo invasion assays. (jneurosci.org)
  • Several lines of evidence suggest that the in vivo transforming activity of oncogenic K-ras is mediated in part through epithelial cell nonautonomous mechanisms. (aacrjournals.org)
  • XIAP overexpression promotes bladder cancer invasion in vitro and lung metastasis in vivo via enhancing nucleolin-mediated Rho-GDIβ mRNA stability. (semanticscholar.org)
  • During influenza viral infection, pDCs are able to prime virus-specific primary and secondary CD4 and CD8 T cell immune responses in vitro and in vivo ( 12 ), but only when the pDCs are exposed to the replicative virus and not with nonreplicating, boiled, or ultraviolet-irradiated virus ( 9 ). (rupress.org)
  • vitro
  • Further analysis of the in vitro kinetics demonstrated a sustained, cell-mediated release of coprecipitated [3H]BSA from BCP ceramics induced by resorbing osteoclasts. (uva.nl)
  • Here, we postulated that stromal cells in the tumor microenvironment alter the tumor cell secretome, including those proteins required for tumor growth and dissemination, and we developed an in vitro model to test this hypothesis. (aacrjournals.org)
  • IL-12(p40)-IgG2b was tested in a murine 2,4,6,-trinitrobenzene sulphonic acid (TNBS) colitis model and in lamina propria mononuclear cells (LPMNC) from patients with CD in vitro. (ebscohost.com)
  • knockdown
  • Similar to PKC-θ-deficient primary CD4 + T cells, small interfering RNA-mediated knockdown of PKC-θ in Jurkat cells also resulted in apoptosis upon TCR stimulation. (jimmunol.org)
  • Forced expression of Bcl-x L was sufficient to inhibit apoptosis observed in PKC-θ knockdown cells. (jimmunol.org)
  • In the present study, we examined the CamKII profile of mouse eggs with a view to using a targeted antisense knockdown approach to determine which CamKII isoform is responsible for cell cycle resumption, and also to determine whether any CamKII-independent pathway could be switched on by a Ca 2+ signal in eggs to similarly induce cell cycle resumption. (biologists.org)
  • migration
  • Overall, these studies establish a trifunctional role for MG53 as a facilitator of rapid injury repair, a mediator of cell migration, and a modulator of myofibroblast differentiation during wound healing. (sigmaaldrich.com)
  • A Travelling Fellowship from Journal of Cell Science allowed her to spend time in Prof Maddy Parson's lab at King's College London, learning new cell migration assays and analysing fibroblasts cultured from individuals with Parkinson's. (biologists.org)
  • Integrins are αβ heterodimeric proteins found on the surface of most mammalian cells and are involved in a wide variety of adhesive functions, such as cell-cell interaction, cell migration, differentiation and adhesion. (embopress.org)
  • mechanisms
  • Non-small cell lung cancer (NSCLC) cells with somatic mutations in K-ras recruit to the tumor a variety of cell types (hereafter collectively termed "stromal cells") that can promote or inhibit tumorigenesis by mechanisms that have not been fully elucidated. (aacrjournals.org)
  • The exquisite sensitivity of the plant root to exogenous Nod factors suggests that these molecules are perceived by receptor-mediated mechanisms. (plantcell.org)
  • Since interleukin-12 is pathogenetically involved in Crohn's disease (CD) but not in ulcerative colitis (UC), expression and mechanisms of induction of interleukin-12 receptor (IL-12R) subunits β1 and β2 were analyzed in lamina propria mononuclear cells (LPMNC) of. (ebscohost.com)
  • Using human umbilical vein endothelial cells (HUVECs), we explored mechanisms underlying the requirement of WNK1-OSR1 signaling for vascular development. (pnas.org)
  • This represents a major step forward for potential stem cell therapies as well as in the basic understanding of the mechanisms that regulate stem cell growth and differentiation. (nextbigfuture.com)
  • T cells therefore have developed complicated mechanisms to balance the survival and apoptotic signals. (jimmunol.org)
  • uptake
  • Chemical cross‐linking studies of purified and surface‐exposed invasin proteins, and the dominant‐interfering effect of a non‐functional invasin derivative are consistent with the presence of a self‐association domain that is located within the region of invasin that enhances bacterial uptake. (embopress.org)
  • endothelial
  • TAMs, vascular endothelial cells, and fibroblasts within the tumor stroma secrete a number of growth factors and chemokines that promote tumorigenesis ( 1 ). (aacrjournals.org)
  • In Kras LA1 mice, lung adenocarcinomas that develop due to somatic activation of a latent oncogenic K-ras allele recruit macrophages, endothelial cells, neutrophils, and fibroblasts, a process driven in part by high expression of CXCR2 ligands ( 7 , 8 ). (aacrjournals.org)
  • We also identified the zinc-finger transcription factor Slug in WNK1-mediated control of endothelial functions. (pnas.org)
  • membrane
  • Modulation of wound healing and scar formation by MG53 protein-mediated cell membrane repair. (sigmaaldrich.com)
  • Cell membrane repair is an important aspect of physiology, and disruption of this process can result in pathophysiology in a number of different tissues, including wound healing, chronic ulcer and scarring. (sigmaaldrich.com)
  • We have previously identified a novel tripartite motif family protein, MG53, as an essential component of the cell membrane repair machinery. (sigmaaldrich.com)
  • The fact that early addition of mAb L8 during cell contact establishment was necessary to be effective may indicate that LRP is a novel plasma membrane protein that plays an early pivotal role in the coordinated metabolic changes which lead to the differentiated phenotype of mature hepatocytes. (rupress.org)
  • Mouse monoclonal to CD38.TB2 reacts with CD38 antigen, a 45 kDa integral membrane glycoprotein expressed on all pre-B cells, plasma cells, thymocytes, activated T cells, NK cells, monocyte/macrophages and dentritic cells. (healthweblognews.info)
  • kinases
  • The association of allergen with IgE bound to FcϵRI trigger signaling cascades leading to activation of kinases, phosphatases, and GTPases, which subsequently induce a variety of events, such as degranulation, cytoskeleton rearrangement, increased gene transcription, and cytokine/chemokine production, in the activated mast cells ( 1 , 2 ). (pubmedcentralcanada.ca)
  • interactions
  • Strategies to inhibit tumor/stromal cell interactions may be useful as therapeutic approaches in NSCLC patients. (aacrjournals.org)
  • However, the roles of these and other cytokines in the complex network of cell-cell interactions in the tumor microenvironment have not been fully elucidated. (aacrjournals.org)
  • A recently published method uses in situ Hi-C followed by chromatin immunoprecipitation (HiChIP) to enrich the overall yield of significant genome-wide interactions mediated by a specific protein. (springer.com)
  • Ando-Kuri M., Rivera I.S.M., Rowley M.J., Corces V.G. (2018) Analysis of Chromatin Interactions Mediated by Specific Architectural Proteins in Drosophila Cells. (springer.com)
  • Small molecules either synthesized within the cell or obtained from the external environment mediate many of these protein-protein interactions. (aacrjournals.org)
  • Complementation strategies for studying protein-protein interactions generally involve the fusion of split reporter protein fragments to the protein of interest in such a way that neither of the fragments retains significant activity by themselves. (aacrjournals.org)
  • This protein fragment-assisted complementation strategy has been used with a variety of reporter proteins, including dihydrofolate reductase, β-Lactamase, green fluorescent protein (GFP), firefly luciferase and Renilla luciferase, and has been found to be a useful technique for studying protein-protein interactions in both bacteria and mammalian cells ( 8 - 12 ). (aacrjournals.org)
  • Recent studies from our lab on protein fragment complementation using split firefly luciferase or split- Renilla luciferase (split-RLuc) have shown their use for studying both protein-protein interactions and small molecule-mediated protein-protein interaction noninvasively in small animals ( 13 , 14 ). (aacrjournals.org)
  • receptor
  • Results: Dimeric IL-12(p40)-IgG2b fusion protein bound specifically to the IL-12 receptor. (ebscohost.com)
  • Increased expression of interleukin-12 receptor β2 on lamina propria mononuclear cells of patients with active Crohn's disease. (ebscohost.com)
  • T cell receptor (TCR)-dependent regulatory T cell (T reg ) activity controls effector T cell (T eff ) function and is inhibited by the inflammatory cytokine tumor necrosis factor-α (TNF-α). (sciencemag.org)
  • Rather, they express a low molecular weight cleavage product, MUC1*, which we previously demonstrated has growth factor receptor-like activity on tumor cells. (nextbigfuture.com)
  • As on cancer cells, MUC1* functions as a growth factor receptor on pluripotent embryonic stem cells. (nextbigfuture.com)
  • FcγRIIa has been described as a potent immune-activating receptor and it contains an ITAM motif, capable of mediating phagocytosis, ADCC, and initiation of inflammatory cytokine release ( 17 ). (rupress.org)
  • differentiation
  • Transition zones between undifferentiated and differentiating cultured stem cells can be found that continue to express OCT4, while also expressing uncleaved, full-length MUC1, which appears to be a marker for the onset of differentiation. (nextbigfuture.com)
  • Thus, the switch from cleaved MUC1* to the full-length protein may be one of the first detectable signals of the onset of differentiation. (nextbigfuture.com)
  • At present this is an imprecise and labor-intensive process that depends on the technician's ability to visually discriminate between cell types then manually dissect pluripotent cells without contaminating the pool with cells that have already entered the differentiation process. (nextbigfuture.com)
  • In fact, stem cell growth supported by the addition of MUC1* ligands to minimal media resisted spontaneous differentiation and produced more pluripotent cells than any other growth condition that we tested. (nextbigfuture.com)
  • Thus, in addition to mediating the growth of embryonic stem cells, MUC1* may be a modulator of differentiation. (nextbigfuture.com)
  • The data presented strongly suggest that MUC1* is a critical marker for the identification and isolation of pluripotent embryonic stem cells as well as a key mediator of the growth and differentiation of pluripotent stem cells. (nextbigfuture.com)
  • Type I interferons activate NK cell cytolytic activity, but protect uninfected cells from NK cell-mediated lysis and affect T cell function by inducing Th1 differentiation ( 5 ). (rupress.org)
  • Thus
  • These results imply that MUC1* may be a more accurate marker of pluripotency than OCT4 and thus antibodies that recognize MUC1* could be used to search for, identify and isolate pure populations of pluripotent stem cells. (nextbigfuture.com)
  • mice
  • A 9 ± 2-fold increase in signal intensity both in cell culture and in living mice was seen compared with a system that expresses both reporter fragments and the interacting proteins separately. (aacrjournals.org)
  • METHODS: FKBP12-deficient and FKBP12.6-deficient T cells were isolated from genetically engineered FKBP12-deficient and FKBP12.6-deficient mice, respectively. (duke.edu)
  • stimulation
  • Stem cells that were cultured in conditioned media from fibroblasts plus bFGF generated a mixture of undifferentiated and differentiated colonies and the colonies were smaller than those produced by MUC1* stimulation. (nextbigfuture.com)
  • Co-stimulation via FcϵRI, using IgE/antigen, and CCR1, using recombinant CCL3/macrophage inflammatory protein-1α (MIP-1α), increased expression of phosphorylated, disassembled, and soluble vimentin in rat basophilic leukemia (RBL)-2H3 cells expressing human CCR1 (RBL-CCR1 cells) and bone marrow-derived murine mast cells, both models of mucosal type mast cells. (pubmedcentralcanada.ca)
  • fusion protein
  • Methods: We generated a fusion protein consisting of the IL-12(p40) subunit fused to the constant region of IgG2b. (ebscohost.com)
  • Conclusions: The IL-12(p40)-IgG2b fusion protein has dichotomic properties as a specific IL-12 antagonist and selective repressor of mucosal inflammation at low concentration and as an IL-12 agonist at high concentration. (ebscohost.com)
  • Different linker peptides in the fusion protein were evaluated for the efficient maintenance of complemented reporter activity. (aacrjournals.org)
  • assay
  • We used a combination of virus overlay protein binding assay and LC-MS/MS, and identified annexin II as a dengue virus serotype 2 (DENV2) interacting protein on Vero cells, upon filopodia induction. (mdpi.com)
  • T-cell growth inhibitory assay was used to assess their responses to immunosuppressant FK506 treatments. (duke.edu)
  • The anticancer effects of 17-DMAG were assessed by an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, a flow cytometric cell-cycle analysis, and an Annexin V-propidium iodide (PI) apoptotic assay. (springer.com)
  • The MTT assay revealed that HT-29 cells were more sensitive to 17-DMAG treatment than DLD-1 cells. (springer.com)
  • Furthermore
  • Furthermore, PKC-θ blockade enhanced T reg function, demonstrating PKC-θ inhibits T reg -mediated suppression. (sciencemag.org)
  • Furthermore, we display that it could function to check the experience of C1 proteins to mediate two embryologic features of Dkk1: induction of chordal and prechordal mesoderm and standards of center cells from non-cardiogenic mesoderm. (healthweblognews.info)
  • antibodies
  • New targets for biologics include cytokines involved in T-cell activation, with antibodies directed against IL-12 and. (ebscohost.com)
  • kinetics
  • Efficient delivery of growth factors from carrier biomaterials depends critically on the release kinetics of the proteins that constitute the carrier. (uva.nl)
  • To this end, tritium-labeled bovine serum albumin ([3H]BSA) was utilized as a model protein to analyze the coprecipitation efficacy and the release kinetics of the protein from the carrier material. (uva.nl)
  • induction
  • Since cleavage of Dkk1 isn't detected, these outcomes define N1 like a book signaling domain name within the undamaged protein that's in charge of the potent ramifications of Dkk1 around the induction and patterning of your body axis and center. (healthweblognews.info)
  • pertussis
  • We recently identified a family of surface-exposed high-molecular-weight proteins of nontypable H. influenzae that are related to filamentous hemagglutinin, a critical adherence factor of Bordetella pertussis. (pnas.org)
  • genes
  • The genes encoding the two such high-molecular-weight proteins (HMW-1 and HMW-2) expressed by a prototypic nontypable H. influenzae strain have now been cloned and sequenced. (pnas.org)
  • antigen
  • Here, we show that human pDCs exploit FcγRII (CD32) to internalize antigen-antibody complexes, resulting in the presentation of exogenous antigen to T cells. (rupress.org)
  • Alternately, activated pDCs induce expansion of antigen-specific memory CD8 + T cells and Th1 CD4 + T cell populations specific for endogenous antigens ( 8 ), influenza virus ( 9 , 10 ), and the MART-1/melan A 26-35 epitope ( 11 ). (rupress.org)
  • antagonists
  • Since IL-12 p40 dimers act as IL-12 antagonists, we assayed p40 dimer proteins to modulate chronic intestinal inflammation. (ebscohost.com)
  • Additional secreted Wnt antagonists, such as for example WIF-1, Crescent, and Frz-B, function by binding and sequestering secreted Wnt protein. (healthweblognews.info)
  • One difference which has surfaced between Dkk1 (and Dkk2 and MK-8776 4) and additional Wnt antagonists is usually that, by binding LRP5/6, Dkk1/2/4 could bias signaling toward the non-canonical Wnt planar cell polarity (PCP) pathway which involves Frizzled however, not LRP receptors. (healthweblognews.info)
  • Deletion from the N1 area impaired advancement of axial mesoderm, however, when portrayed as indie proteins, N1 synergized with C1 and various other Wnt antagonists to market advancement of chordal and prechordal mesoderm. (healthweblognews.info)
  • dendritic cells
  • For example, dendritic cells in the microenvironment present tumor antigens to lymphocytes (CD4 + , CD8 + , and natural killer), promoting an antitumor response. (aacrjournals.org)
  • Expression
  • We found that loss of expression of HMW-1 by the prototypic strain and a HMW-1-like protein in a heterologous nontypable H. influenzae strain markedly decreased the capacity to adhere. (pnas.org)
  • TRAIL decoy receptors were not detected, suggesting that the resistance of 2101 and MCF-7/ADR cells, strongly expressing PrPc, to TRAIL-mediated cell death was independent from the expression of TRAIL receptors and death-inducing signaling complex formation. (aacrjournals.org)
  • Classic multidrug resistance is attributed to the elevated expression of ATP-dependent drug-efflux pumps ABCB1 (also known as P-glycoprotein), ABCC1 (also known as multidrug resistance-associated protein) and ABCG2 (also known as breast cancer-resistance protein and mitoxantrone-resistance protein), all of which belong to the superfamily of ATP-binding cassette transporters (for review, see ref. 3 ). (aacrjournals.org)
  • Therefore, the current model is that PI3K/Akt pathway-mediated activation of NF-κB is responsible for stimulating Bcl-x L expression, resulting in enhanced survival of T cells. (jimmunol.org)
  • The level of GRP78 expression may determine the therapeutic efficacy of 17-DMAG against colon cancer cells. (springer.com)
  • inflammation
  • Connective tissue cells actively control interstitial fluid pressure (IFP), and inflammation-induced lowering of IFP provides a driving force for edema formation. (diva-portal.org)