• Rhamnaceae) on cisplatin-induced damage to kidney epithelial LLC-PK1 cells via mitogen-activated protein kinase (MAPK) and apoptosis pathways. (mdpi.com)
  • Caspase-mediated cleavage of focal adhesion kinase disassembly and pp125FAK of focal adhesions in individual endothelial cell apoptosis. (opioid-receptors.com)
  • PubMed]Murata-Hori M, Fukuta Y, Ueda K, Iwasaki T, Hosoya H. HeLa Squat kinase induces diphosphorylation of myosin II regulatory light chain and reorganization of actin filaments in nonmuscle cells. (opioid-receptors.com)
  • PubMed]Murata-Hori M, Suizu F, Iwasaki T, Kikuchi A, Hosoya H. Squat kinase identified as a novel myosin regulatory light chain kinase in HeLa cells. (opioid-receptors.com)
  • Phosphorylation by protein kinase C stabilizes ABCG1 and increases cholesterol efflux. (kyoto-u.ac.jp)
  • CD95 signaling by protein kinase R-like endoplasmic reticulum kinase causes activation was responsible for the F Promotion of both caspase-8 association erismodegib LDE225 with CD95 and increased Ht eIF2 phosphorylation. (syksignaling.com)
  • Activator of protein kinase A (cyclic AMP agonist). (biolog.de)
  • Representative compounds from each class of drugs were further tested as Pgp substrates, defined by the ability of human Pgp or mouse mdr1a/Pgp to transport them across a polarized kidney epithelial cell in vitro. (nih.gov)
  • Although previously believed to be constitutively expressed exclusively in liver, the present study demonstrates the induction of this gene by oxalate in porcine proximal tubular LLC-PK1 cells and rat kidney. (betalifesci.com)
  • The alpha(1)-microglobulin and bikunin proteins are accordingly present in developing hepatocytes, pancreas, kidney, and gut. (betalifesci.com)
  • Impact of high glucose and AGEs on cultured kidney-derived cells. (unifesp.br)
  • The effects of high glucose (HG) and advanced glycation end products (AGEs) on cell viability, lysosomal enzymes and other effectors of cell signaling of cultured kidney cells were studied. (unifesp.br)
  • We are taking comprehensive approaches and applying multiple model systems, including in vitro 3D culture, in vivo live image technology, in vitro organ culture, in vivo live image technology, in vitro organ culture/tissue slice culture, isolated kidney perfusion, mouse models and zebrafish models to study protein trafficking/epithelial polarity/remodeling in various kidney disease contexts. (massgeneral.org)
  • The effects of 3, 3', 4, 4', 5-pentachlorobiphenyl (PCB-126), which is the most toxic congener of coplanar polychlorinated biphenyls (Co-PCBs), on intracellular accumulation and transepithelial transport of vinblastine were examined in porcine kidney cells, LLC-PK1, and its transformant cells expressing human P-glycoprotein (LLC-MDR1). (go.jp)
  • We found that "knockout" mice lacking one of these transport proteins have a high incidence of calcium oxalate urinary stones, the same type that is most common in human patients with kidney stones. (yale.edu)
  • We found that this kidney transport protein also plays a very crucial role in the intestine, where it secretes oxalate and thereby limits how much of ingested oxalate is absorbed and then excreted in the urine. (yale.edu)
  • cell damage in the kidney cell range LLC-PK1, porcine aortic endothelial rat or cells hepatocytes. (techblessing.com)
  • The goal of this study was to investigate constitutive differences in the susceptibility of renal cortex cells originating from human, rat and porcine origin in vitro. (uni-konstanz.de)
  • AA cytotoxicity was observed in all cell types in a time- and concentration dependent manner with species-specific differences, with porcine cells being the most sensitive. (uni-konstanz.de)
  • AA had a comparable effect on the cell cycle in primary human and porcine cells and the rat NRK-52E cell line following 48 h exposure, also corroborated by the reduced 3H-thymidine incorporation in NRK-52E cells. (uni-konstanz.de)
  • In addition, DNA unwinding, suggestive of enhanced DNA damage, was observed in primary porcine cells. (uni-konstanz.de)
  • Pgp antagonism was defined by interactions of the drugs with four cell lines (LLC-PK1, L-MDR1, L-mdr1a, and L-mdr1b) using a microfluorometric calcein-AM assay and characterized for their inhibitor constant (K(i)) toward calcein-AM. The compounds were further defined for their ability to inhibit MDR1 by their effect on vinblastine accumulation into L-MDR1 cells. (nih.gov)
  • The uptake of [ 3 H]vinblastine, a substrate of P-gp, by Caco-2 cells was measured. (aspetjournals.org)
  • The ethyl acetate extract of orange juice and these methoxyflavones also increased steady-state [ 3 H]vinblastine uptake by LLC-GA5-COL300 cells (a cell line transfected with human MDR1 cDNA). (aspetjournals.org)
  • In kidneys suffering from ischemia-reperfusion and in renal tubular epithelial cells subjected to hypoxia/reoxygenation, NCX extrudes Na + in exchange for Ca 2+ influx, resulting in intracellular Ca 2+ overload and tubular epithelial cell injury (Yamashita J, S Kita, T Iwamoto et al. (cellphysiolbiochem.com)
  • We are also interested in studying the mechanism of renal epithelial cell regeneration and differentiation during tubular repair/remodeling after tubular injury. (massgeneral.org)
  • The most widely used renal epithelial cell lines of animal origin are the LLC-PK1 (Hampshire pig) and OK (American opossum) cell lines, exhibiting characteristics suggestive of proxi-mal tubular origin, and the MDCK (Cocker Spaniel) cell line, exhibiting characteristics suggestive of distal origin. (pharmacy180.com)
  • The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. (betalifesci.com)
  • Global sequencing of proteolytic cleavage sites in apoptosis by specific labeling of protein N termini. (opioid-receptors.com)
  • EP reduced PDCoV-induced apoptosis and enhanced tight junction protein expressions in the small intestine, maintaining the integrity of the intestinal barrier. (biomedcentral.com)
  • It can reduce LLC-PK1 cells apoptosis caused by PDCoV infection. (biomedcentral.com)
  • Thus, the effects of EP on modulating PDCoV-induced apoptosis and the impact of PDCoV on TJ proteins need to be elucidated in vivo. (biomedcentral.com)
  • The aim of this study was to test for the molecular and functional expression of the non-gastric H + /K + ATPase ATP12A/ATP1AL1 in unstimulated and butyrate-stimulated (1 and 10 mM) human myelomonocytic HL-60 cells, to unravel its potential role as putative apoptosis-counteracting ion transporter as well as to test for the effect of the H + /K + ATPase inhibitor SCH28080 in apoptosis. (karger.com)
  • No significant effect on the uptake of 3- O -[ 3 H]methylglucose or [ 14 C]phenylalanine by Caco-2 cells was found, compared with the control. (aspetjournals.org)
  • Even when released, they remain largely membrane associated due to their hydrophobic nature and can be taken up by cells via a high-affinity uptake mechanism [ 23 ], which is followed by their enzymatic degradation. (degruyter.com)
  • Recently, for example, in vitro studies involving renal cells in culture suggested that the underlying mechanism of the proteinuria associated with the use of rosuvastatin was inhibition by the statin of the endocytotic uptake of proteins by the proximal tubular cell (Verhulst, D'Haese and De Broe, 2004). (pharmacy180.com)
  • High-affinity zinc uptake system protein mutant ( znuA mutant) showed reduced growth in zinc chelated medium, and failed to replicate in HeLa cells and mouse bone marrow-derived macrophages. (go.jp)
  • Energetic separation of chromatin and endoplasmic reticulum into blebs in past due apoptotic cells. (opioid-receptors.com)
  • ADPLD results from mutations in PRKCSH or SEC63, genes that code the glucosidase II² (GII²) and SEC63p, proteins related to the endoplasmic reticulum. (fapesp.br)
  • Both CB 1 and CB 2 receptors, which share a low level (44%) of sequence homology [6], are G protein-coupled receptors that mainly signal via G i /G o proteins, even though they may also activate G s , G q/11 , and G protein-independent signaling pathways [ 7 ]. (degruyter.com)
  • Using the endothelin-1 (ET-1)-stimulated elevation in cGMP in LLC-PK1 cells as a biological detector system for the conversion of big ET-1 (bET-1) to ET-1, we detected bET-1-converting activities in subcellular fractions from bovine aortic cultured endothelial cells (BAE) and rat brain. (aspetjournals.org)
  • Quantitative targeted absolute proteomic analysis of 31 membrane proteins showed that P-glycoprotein (P-gp/mdr1a) protein expression levels were significantly increased in brain capillaries of PTZ (129%), EL (143%), and PHT mice (192%) compared with controls. (aspetjournals.org)
  • HG increased viable mesangial cells (ihMC) in 48 h, while epithelial tubular cells were not affected (LLC-PK1 and MDCK). (unifesp.br)
  • We used cell lines expressing human, mouse, and rat mdr1 genes. (nih.gov)
  • The purpose of this study was to demonstrate experimentally that alterations of in vivo transporter function at the blood-brain barrier (BBB) in disease and during pharmacotherapy can be reconstructed from in vitro data based on our established pharmacoproteomic concept of reconstructing in vivo function by integrating intrinsic transport activity per transporter molecule and absolute protein expression level at the BBB. (aspetjournals.org)
  • However, advances in cell and tissue culture will permit the development of in vitro toxicity assays. (pharmacy180.com)
  • The aim of the development of in vitro tests is not only to replace in vivo animal testing but also to study the mechanisms of cell modulation by toxic compounds. (pharmacy180.com)
  • To investigate whether PC1 resulting from missense mutations are amenable to rescue by chaperone therapy, two patient-based mutations were chosen: p.R2220W and p.E2771K.2) To perform in vitro studies evaluating expression and trafficking of mutant Polycystin-1: The inactivation of endogenous Pkd1 and Pkd2 in LLC-PK1 cells was developed as a functional bioassay to address expression and trafficking of mutant PC1 by using fluorescent staining methods. (fapesp.br)
  • In addition, we studied the stability of both isomers in aqueous solution and compared their antiproliferative activity in vitro in three human cancer cell lines CH1 (ovarian carcinoma), SW480 (colon carcinoma) and A549 (non-small Dimethyl sulfoxide cell lung cancer) and in vivo in a Hep3B SCID mouse xeno-transplantation model in order to establish whether tautomer identity in 1 and 2 has any effect on biological properties. (erksignal.com)
  • In the renal tubular cells, A1M and bikunin co-precipitate, indicating lack of cleavage of the precursor protein. (betalifesci.com)
  • Under normal conditions, a minute amount of low molecular weight proteins are filtered, which then undergo endocytic reabsorption by prox-imal tubular cells. (pharmacy180.com)
  • For exam-ple, alanine aminopeptidase, alkaline phosphatase and glutamyltransferase are enzymes bound to the brush border of proximal tubular cells. (pharmacy180.com)
  • These results show the presence of at least two different endothelin-converting enzyme activities in endothelial cells and a third within the rat brain. (aspetjournals.org)
  • Exposure to HG had no effect on the cathepsin B activity, but AGE-BSA caused a marked decrease in LLC-PK1, and increased the enzyme activities in the other cell lines. (unifesp.br)
  • In vivo P-gp/mdr1a function at the BBB was reconstructed from the measured P-gp/mdr1a protein expression levels and intrinsic transport activity for verapamil per P-gp/mdr1a previously reported by our group. (aspetjournals.org)
  • A complementary approach uses mice with targeted gene disruption to elucidate the physiological roles of ion exchangers and associated proteins under in vivo conditions. (yale.edu)
  • The antiproliferative activity of (H2ind)[OsIVCl5(2H-ind)] (1) was found to be superior to that of (H2ind)[OsIVCl5(1H-ind)] (2) in one of three human cancer cell lines applied but inferior in the in vivo xeno-transplantation model. (erksignal.com)
  • INTRODUCTION Apoptotic cell death is usually an indispensable process for embryonic development and maintenance of tissue homeostasis in multicellular organisms (Jacobson and for 10 min at 4?C. The cell lysate was incubated with anti-V5 antibody bound to Dynabeads Protein G (Invitrogen) under constant rotation for 1 h. (opioid-receptors.com)
  • Type I angiotensin II receptors (AT1R) are somewhat unique in that they are expressed at apical (AP) and basolateral (BL) membranes in proximal tubule cells and both receptor sites undergo endocytosis. (duke.edu)
  • In liver, the precursor protein is cleaved in the Golgi network by a furin-like enzyme to release constituent proteins, which undergo glycosylation before their export from the cell. (betalifesci.com)
  • As mTOR induces anabolism and inhibits autophagy, and TFEB is a master transcription factor for lysosomal enzymes, it is possible that this pathway plays a role in the inhibition of lysosomal enzymes in proximal tubule cells. (unifesp.br)
  • They also demonstrate the use of LLC-PK1 cells as a rapid assay that permits the sensitive detection and measurement of the formation of biologically active ET-1 from its precursor bET-1. (aspetjournals.org)
  • The protein concentration was determined utilizing a Bio Rad protein assay kit. (wee1signaling.com)
  • Dedication of IC50 of AKR1B10 inhibitors The em IC /em 50-value of the inhibitors were identified using the assay combination comprising 0.1 M sodium phosphate buffer (pH 7.5), 7.5 mM DL-glyceraldehyde, 0.2 mM NADPH, 0.3 M AKR1B10 wild-type protein and varying concentrations of inhibitors depending on their inhibition potency. (tam-receptor.com)
  • This review focuses on emerging topics encompassing the functional involvement of aquaporin channel proteins (AQPs) and membrane transport systems, also allowing permeation of NO and hydrogen peroxide, a major ROS, in oxidative stress physiology and pathophysiology. (hindawi.com)
  • Phosphorylation events and the modulation of aquaporin 2 cell surface expression. (massgeneral.org)
  • In these cells the myosin-1a protein localizes specifically to the brush border. (wikipedia.org)
  • Experiments indicate that the brush border population of the encoded protein turns over rapidly, while its head and tail domains interact transiently with the core actin and plasma membrane, respectively. (wikipedia.org)
  • The microtubule cytoskeleton is critical for muscle cell differentiation and undergoes reorganisation into an array of paraxial microtubules, which serves as template for contractile sarcomere formation. (elifesciences.org)
  • We show that oMAP4 is expressed upon muscle cell differentiation and is the only MAP4 isoform essential for normal progression of the myogenic differentiation programme. (elifesciences.org)
  • Upon differentiation, mono-nucleated myoblasts withdraw from the cell cycle and fuse to form syncytial myotubes ( Wakelam, 1985 ). (elifesciences.org)
  • Normally, activation of the CB 1 receptor regulates renal vascular hemodynamics and stimulates the transport of ions and proteins in different nephron compartments. (degruyter.com)
  • Inhibition kinetics of daunorubicin reduction by AKR1B10 The inhibition kinetics of daunorubicin reduction by histagged AKR1B10 wild-type protein was monitored spectrophotometrically, by measuring decrease in the absorbance of the cofactor NADPH at 340 nm (Balendiran and Rajkumar, 2005, Martin et al. (tam-receptor.com)
  • Characterization of endothelin-converting enzyme from endothelial cells and rat brain: detection of the formation of biologically active endothelin-1 by rapid bioassay. (aspetjournals.org)
  • In the brain, rMdr1a was found to be expressed on the luminal (apical) membrane of capillary endothelial cells, whereas rMdr1b was detected in the parenchyma [13]. (solvobiotech.com)
  • Similarly, bioactive peptides, which are specific protein fragments that are thought to have physiological health benefits, are good candidates for cosmeceutical components that provide enzyme-modulating activity. (dovepress.com)
  • Determination of the quantity and the quality of urinary proteins allows for the distinction between 'glomerular' and 'tubular' proteinuria. (pharmacy180.com)
  • This review focuses on an emerging topic, the functional involvement of AQPs in ROS membrane transport, with specific regard to the movement of hydrogen peroxide and NO into and out of cells, in both health and oxidative stress-induced diseases. (hindawi.com)
  • The levels of nitric oxide (NO) was increased by AGE-BSA in all cell lines, suggesting oxidative stress, and Western blotting has shown that, among the investigated proteins, cathepsin B, mTOR and transcription factor EB (TFEB) were the most significantly affected by exposure to AGE-BSA. (unifesp.br)
  • In various mouse and rat models of obesity and type 1 and 2 diabetes mellitus, eCBs generated in various renal cells activate CB 1 receptors and contribute to the development of oxidative stress, inflammation, and renal fibrosis. (degruyter.com)
  • As the expression of the AMBP gene is regulated by A1M-specific cis elements and transcription factors, A1M protein was studied as a representative of AMBP gene expression in renal cells. (betalifesci.com)
  • It was shown that SEC63p deficiency activates the IRE1±-XBP1 branch of UPR, resulting in up-regulated transcription of chaperone proteins, which work to overcome the burden of unfolded proteins. (fapesp.br)
  • The existence of specific receptors for plant-derived molecules in mammalian cells initiated a search for specific endogenous ligands. (degruyter.com)
  • You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. (betalifesci.com)
  • Aims: To address whether reduced functional PC1 resulting from missense mutations are amenable to rescue by chaperone therapy.Specific aims:1) To generate modified cell lines and mouse models using genome-editing technology. (fapesp.br)
  • The myosin-Ia protein is expressed by enterocytes, the epithelial cells that line the luminal surface of the small intestine. (wikipedia.org)
  • Each of the cell lines had been derived from Japanese sufferers. (wee1signaling.com)
  • The initially established six CC cell lines, HuCCT1 and MKN45 were maintained in RPMI with ten bovine serum. (wee1signaling.com)
  • In contrast, the number of viable cells was markedly decreased, for all cell lines, by AGE-BSA. (unifesp.br)
  • Several permanent and immortalised cell lines of human and non-human origin are available, offer-ing several advantages over primary cultures such as an unlimited life span and the lack of time-consuming isolation procedures. (pharmacy180.com)
  • We plan to develop modified cell lines and mouse models using CRISPR-Cas9 technology. (fapesp.br)
  • Differences between human, rodent and other species were also found with regard to the inhibitory effects of quinidine and verapamil on Mdr1-mediated daunorubicin, digoxin and cyclosporin A transport in Mdr1-transfected cell lines [30]. (solvobiotech.com)
  • Background In contemporary biotechnology, there is a want for pausing cell lines by cool storage space to adapt large-scale cell cultures to the adjustable demand for their items. (techblessing.com)
  • Their protein structure is highly similar to human MDR1: mouse Mdr1a and Mdr1b share 87% and 80% amino acid homology, respectively, with human MDR1, while rat Mdr1 transporter amino acid sequences are 80% homologous to human [7], [9]. (solvobiotech.com)
  • Overexpression of human MDR1 versus mouse Mdr1a or Mdr1b in CHO cells conferred different levels of drug resistance due to the distinct capacities of these transporters [27]. (solvobiotech.com)
  • Cleavage of structural protein during the set up of the essential contraindications mind of bacteriophage Testosterone levels4. (opioid-receptors.com)
  • Indirect immunofluorescence Indirect immunofluorescence was carried out as described previously (Murata-Hori cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme. (opioid-receptors.com)
  • The accumulation decreased less than one-tenth in LLC-MDR1 compared to LLC-PK1. (go.jp)
  • In both cells, the accumulation increased with the addition of PCB-126 and cyclosporine A (CYA), which are P-glycoprotein modulators, though the magnitudes were different in these two cell groups as well as for these two chemicals. (go.jp)
  • Inhibition of Ced-3/ICE-related Acacetin IC50 proteases does not prevent cell death induced by oncogenes, DNA damage, or the Bcl-2 homologue Bak. (opioid-receptors.com)
  • Inhibition by berberine of cyclooxygenase-2 transcriptional activity in human colon cancer cells. (ppt-health.com)
  • Transformation of znuA mutant with a shuttle vector pBBR1MCS-4 containing znuA gene restored the growth in zinc chelated medium and intracellular replication in HeLa cells and macrophages to a level comparable to that of wild-type strain. (go.jp)
  • Bacterial internalization into HeLa cells and macrophages and co-localization with either late endosomes or lysosomes of znuA mutant were not different from those of wild-type strain. (go.jp)
  • Unconventional myosin-Ia is a protein that in humans is encoded by the MYO1A gene. (wikipedia.org)
  • The protein encoded by this gene belongs to the myosin superfamily. (wikipedia.org)
  • The alpha(1)-microglobulin/bikunin precursor (AMBP) gene, and its two protein products were studied in mouse embryos of 8.5-15.5 days of embryonic development by in situ hybridization and immunohistochemistry. (betalifesci.com)
  • Roles of the Mesenchymal Stromal/Stem Cell Marker Meflin in Cardiac Tissue Repair and the Development of Diastolic Dysfunction. (kyoto-u.ac.jp)
  • Rat Mdr1 proteins display a slightly different tissue distribution compared to human or mouse. (solvobiotech.com)
  • Cisplatin-induced LLC-PK1 cell death was most significantly reduced following treatment with 3-dehydroxyceanothetric acid 2-methyl ester (3DC2ME). (mdpi.com)
  • This notion has been challenged by the discovery of new membrane transport functions, especially those exerted by aquaporins (AQPs), a family of membrane channel proteins widespread in nature [ 10 , 11 ]. (hindawi.com)
  • Transport of NO and ROS by AQPs would be required for cell homeostasis to play a critical role in maintaining endothelial function. (hindawi.com)
  • Our lab has specifically focused on identifying the proteins involved in mediating the transport of bicarbonate, NaCl and ammonium in the proximal tubule. (yale.edu)
  • Our work is primarily focused on membrane transport proteins mediating ion exchange, namely NHE isoforms mediating Na + -H + exchange, and SLC26 isoforms mediating anion exchange. (yale.edu)
  • Transporters such as organic anion-transporting polypeptide 1A2, P-glycoprotein, human organic cation transporter 1, and multidrug and toxin extrusion protein 1 are involved in the pharmacokinetics of LVX. (dovepress.com)
  • Endocytosis modulates cell responses by removing and recycling receptors from the cell surface. (duke.edu)
  • The dephosphorylation activity of Rabbit Polyclonal to HUNK myosin phosphatase immunoprecipitated from the apoptotic cells was lower than that from the nonapoptotic control cells. (opioid-receptors.com)
  • The latter but not the former activity was inhibited in a concentration-dependent manner by phosphoramidon (approximate IC50, 1 microM) and converted bET-1 to ET-1 at a rate of 0.6 nmol/hr/mg of protein. (aspetjournals.org)
  • Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. (betalifesci.com)
  • The assessment of kinetic guidelines for wild-type and C299S mutant AKR1B10 shows that substitution of serine by cysteine at position 299 reduces the protein affinity for DL-glyceraldehyde and enhances its catalytic activity. (tam-receptor.com)
  • A rapidly exchanging pool of the unconventional myosin-Ia protein binds to the actin core bundle, which turns over on a much slower timescale. (wikipedia.org)
  • Concerning lysosomal enzymes, the main cysteine-protease expressed by these cells was cathepsin B, and its concentration was much higher in epithelial than in mesangial cells. (unifesp.br)
  • Magnesium is involved in protein and nucleic acid synthesis, bone growth, energy metabolism, regulation of blood pressure, and in the activation of hundreds of enzymes [3,4]. (milkgenomics.org)
  • Live Cell FRET Analysis of the Conformational Changes of Human P-glycoprotein. (kyoto-u.ac.jp)
  • Under normal conditions, the glomerular filtration barrier restricts the transfer of high molecular weight proteins from the plasma to the lumen of the tubule. (pharmacy180.com)
  • High molecular weight proteins appearing in the urine points to a pathological condi-tion of the glomerulus, changing the permselectiv-ity of the filter. (pharmacy180.com)
  • We analyzed AT1R cytoplasmic (-COOH) tail deletion mutants to determine whether classic AT1R endocytosis motifs functioned similarly in polarized cells and simultaneously altered receptor properties. (duke.edu)
  • AT(1)R in polarized cells contain dominant endocytosis signals but these motifs do not correlate with AP or BL AT1R expression. (duke.edu)
  • At the C-terminus, each myosin class has a distinct tail domain that serves in dimerization, membrane binding, protein binding, and/or enzymatic activities and targets each myosin to its particular subcellular location. (wikipedia.org)
  • Depending on the cell density, exposed fish showed behavioural changes, including increased ventilation rates possibly representing a physiological stress response. (uni-konstanz.de)
  • Throughout the years, ROS and NO have been widely considered to enter cells by freely diffusing through the cell membrane lipid bilayer and not via specific transporters or channels. (hindawi.com)