SynthaseEnzymeDehydrogenaseFatty acidPeptidyl-carrierFAALLipoateEscherichiaGeneSerine residueAminoLipoic acidMoleculePathwaySubstrateEnzymesBiologicalKinaseExperimentalMolecularEnsemblInterproscanTransporterReductaseLong-chain-TRNAHypothetical proteinSubunitUbiquitinBiotinRibosomal proteinSynthesisDomain-containMoleculeGenesMembraneAmino acidsBacteriaCovalent attachmentBiosyntheticCarboxylaseCytochromeCharacterizationSequenceMitochondriaTransmembraneCytoplasmAcyltransferaseResidues
Synthase3
- Therefore, molecular docking was also used in the present study to simulate the binding of cordycepin with different antibacterial targets, such as β-ketoacyl-(acyl-carrier-protein) synthase III (FabH), DNA gyrase B and thymidylate kinase, in order to identify the most likely antimicrobial mechanism of cordycepin. (spandidos-publications.com)
- Risperidone upregulates fatty acid synthase (FASN) and sterol regulatory element-binding protein 1 (SREBP1) expression in hepatocyte cultures and mouse liver by targeting the hepatic SREBP-1c/FASN couple, which is also one of the mechanisms by which risperidone induces weight gain ( 24 ). (frontiersin.org)
- The enzyme is capable of transferring the 4'-phosphopantetheine moiety of coenzyme A to a conserved serine residue in both the acyl carrier protein domain of the human cytosolic multifunctional fatty acid synthase and the acyl carrier protein associated independently with human mitochondria. (rhea-db.org)
Enzyme7
- Butirosin acyl-carrier protein)-L-glutamate ligase (EC 6.2.1.39, [BtrI acyl-carrier protein]-L-glutamate ligase, BtrJ) is an enzyme with systematic name (BtrI acyl-carrier protein):L-glutamate ligase (ADP-forming). (wikipedia.org)
- GapMind searches the predicted proteins for candidates by using ublast (a fast alternative to protein BLAST) to find similarities to characterized proteins or by using HMMer to find similarities to enzyme models (usually from TIGRFams ). (lbl.gov)
- Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam ). (lbl.gov)
- The enzyme attaches lipoic acid to the lipoyl domains of certain key enzymes involved in oxidative metabolism, including pyruvate dehydrogenase (E 2 domain), 2-oxoglutarate dehydrogenase (E 2 domain), the branched-chain 2-oxoacid dehydrogenases and the glycine cleavage system (H protein) [6]. (enzyme-database.org)
- An enzyme is a biological macromolecule made up of proteins. (pediaa.com)
- An enzyme refers to a substance produced by living organisms to act as a biological catalyst to bring about a specific biochemical reaction while a coenzyme refers to a non-protein compound necessary for the functioning of an enzyme. (pediaa.com)
- The human enzyme, which is expressed mainly in the cytosolic compartment in a wide range of tissues, is a 329-residue, monomeric protein. (rhea-db.org)
Dehydrogenase1
- The acyl carrier protein of lipid synthesis donates lipoic acid to the pyruvate dehydrogenase complex in Escherichia coli and mitochondria. (enzyme-database.org)
Fatty acid6
- These enzymes are found in a variety of biosynthetic contexts, which include fatty-acid activating enzymes, type I or type III polyketide synthases, dialkylresorcinol-generating enzymes, monooxygenases or Rieske proteins. (biomedcentral.com)
- Through a series of minimally complex analogs, we determined non-proteinogenic amino acids and the N-acyl fatty acid moiety are essential for bioactivity. (duke.edu)
- Medium-chain-fatty-acid--CoA ligase (EC 6.2.1. (lbl.gov)
- Acyl carrier protein (ACP) is an essential cofactor in the synthesis of fatty acids by the fatty acid synthetases systems in bacteria and plants. (embl.de)
- Fatty acid transporter proteins (FATPs) are a family (SLC27) of six transporters (FATP1-6). (guidetopharmacology.org)
- Intersecting analysis, molecular docking, and pathway validation analysis showed that risperidone influences the adipocytokine signaling pathway by targeting MAPK14 (mitogen-activated protein kinase 14), MAPK8 (mitogen-activated protein kinase 8), and RXRA (retinoic acid receptor RXR-alpha), thereby inhibiting long-chain fatty acid β-oxidation by decreasing STAT3 (signal transducer and activator of transcription 3) expression and phosphorylation. (frontiersin.org)
Peptidyl-carrier1
- The human 4'-phosphopantetheine transferase is also capable of phosphopantetheinylation of peptidyl carrier and acyl carrier proteins from prokaryotes. (rhea-db.org)
FAAL1
- Through a series of bioactivity assays, we found the transfer to the acyl carrier protein (ACP) in a fatty acyl-AMP ligase (FAAL)-dependent manner determined the specificity of lipids selected in the biosynthetic process. (duke.edu)
Lipoate2
- Lipoate protein ligase B primarily recognizes the C8-phosphopantetheine arm of its donor substrate and weakly binds the acyl carrier protein. (du.ac.in)
- Morris, T.W., Reed, K.E. and Cronan, J.E., Jr. Identification of the gene encoding lipoate-protein ligase A of Escherichia coli . (enzyme-database.org)
Escherichia3
- protein_coding" "AAC73960","hcp","Escherichia coli","hybrid-cluster [4Fe-2S-2O] subunit of anaerobic terminal reductases [Ensembl]. (ntu.edu.sg)
- protein_coding" "AAC74319","hns","Escherichia coli","global DNA-binding transcriptional dual regulator H-NS [Ensembl]. (ntu.edu.sg)
- The codon table used was the FreqB table from " Design Parameters to Control Synthetic Gene Expression in Escherichia coli ", which was used because it was shown to express proteins well and was recommended to us by JCVI. (freegenes.org)
Gene3
- There are many possible applications for these genes: you could try to recreate essential gene pathways in-vitro, or purify the proteins for functional studies, or create a synthetic MoClo-compatible Bacillus subtilis genome using these genes and the sgRNAs available from the Bacillus Genetic Stock Center. (freegenes.org)
- select ( V1 , V2 , V11 , Protein.names , Gene.Ontology. (github.io)
- V1" "V2" "V11" "Protein.names" "Gene.Ontology. (github.io)
Serine residue1
- Phosphopantetheine is attached to a serine residue in these proteins. (embl.de)
Amino1
- Twenty alpha-amino acids are the subunits which are polymerized to form proteins. (lookformedical.com)
Lipoic acid4
- The lipoic acid salvage pathway is composed of two lipoic acid ligases, LplA1 and LplA2, that sequester environmental lipoic acid. (luc.edu)
- The octanoic acid is converted to lipoic acid, and LipL facilitates the transfer to the bacterial metabolic protein. (luc.edu)
- While this is valuable in understanding ligase activity, it does not necessarily model the host environment in which there is limited free lipoic acid. (luc.edu)
- This led me to ask if LplA1 and LplA2 ligases can use lipoamide as a source of lipoic acid for protein lipoylation. (luc.edu)
Molecule1
- Search proteins in UniProtKB for this molecule. (rhea-db.org)
Pathway1
- The de novo biosynthesis pathway allows for the transfer of octanoic acid from an acyl carrier protein to GcvH. (luc.edu)
Substrate3
- LysR substrate binding domain, Bacterial regulatory helix-turn-helix protein [Interproscan]. (ntu.edu.sg)
- ABC transporter ATP-binding protein-unknown substrate. (ntu.edu.sg)
- However, it is also used in other cellular processes, notably as a substrate of enzymes that add or remove chemical groups from proteins, in posttranslational modifications . (wikidoc.org)
Enzymes2
- Most enzymes are globular proteins with high molecular weights. (pediaa.com)
- Enzymes are mainly globular proteins while coenzymes are organic molecules. (pediaa.com)
Biological1
- It causes the production of abnormal proteins with impaired biological activity. (lookformedical.com)
Kinase1
- Receptor-like protein kinase. (or.jp)
Experimental1
- Hits to curated proteins without experimental data as to their function are never considered high confidence. (lbl.gov)
Molecular1
- The results of computational molecular docking analysis indicated that the interaction energy between cordycepin and NAD+‑dependent DNA ligase (LigA) was lower than that between cordycepin and other common antibacterial targets. (spandidos-publications.com)
Ensembl3
- ABC-type transport system, ATP-binding protein [Ensembl]. (ntu.edu.sg)
- Cell division ATP-binding protein FtsE [Ensembl]. (ntu.edu.sg)
- Taurine import ATP-binding protein TauB [Ensembl]. (ntu.edu.sg)
Interproscan11
- Ribosomal protein S20 [Interproscan]. (ntu.edu.sg)
- D-ala D-ala ligase C-terminus, D-ala D-ala ligase N-terminus [Interproscan]. (ntu.edu.sg)
- Bor protein [Interproscan]. (ntu.edu.sg)
- Protein of unknown function (DUF1158) [Interproscan]. (ntu.edu.sg)
- Glutamate-cysteine ligase family 2(GCS2) [Interproscan]. (ntu.edu.sg)
- Protein of unknown function (DUF2526) [Interproscan]. (ntu.edu.sg)
- Ribosomal protein L35 [Interproscan]. (ntu.edu.sg)
- Protein of unknown function (DUF441) [Interproscan]. (ntu.edu.sg)
- YebO-like protein [Interproscan]. (ntu.edu.sg)
- Protein of unknown function (DUF2511) [Interproscan]. (ntu.edu.sg)
- Copper resistance protein D [Interproscan]. (ntu.edu.sg)
Transporter1
- ABC transporter ATP-binding protein & cell division. (ntu.edu.sg)
Reductase1
- 14478 3-oxoacyl-[acyl-carrier protein] reductase fabG BBZA01000001 CDS ARMA_0014 complement(14430. (go.jp)
Long-chain-1
- This reaction is facilitated by the long-chain fatty-acid CoA ligase 1 protein, which adds a CoA moiety to appropriate acyl groups. (smpdb.ca)
TRNA3
- 16723) hypothetical protein BBZA01000003 tRNA ARMA_tRNA01 complement(16745. (go.jp)
- protein_coding" "Cz03g05060.t1","No alias","Chromochloris zofingiensis","tRNA methyltransferase TRMD/TRM10-type domain [Interproscan]. (ntu.edu.sg)
- protein_coding" "Cz08g16110.t1","No alias","Chromochloris zofingiensis","tRNA (1-methyladenosine) methyltransferase catalytic subunit Gcd14 [Interproscan]. (ntu.edu.sg)
Hypothetical protein44
- 4628 hypothetical protein BBZA01000001 CDS ARMA_0005 5077. (go.jp)
- 5685 hypothetical protein BBZA01000001 CDS ARMA_0006 5873. (go.jp)
- 7021 hypothetical protein BBZA01000001 CDS ARMA_0007 complement(7097. (go.jp)
- 7504) hypothetical protein BBZA01000001 CDS ARMA_0008 complement(7590. (go.jp)
- 11958) hypothetical protein BBZA01000001 CDS ARMA_0012 12170. (go.jp)
- 578 hypothetical protein BBZA01000002 CDS ARMA_0016 684. (go.jp)
- 2342 hypothetical protein BBZA01000002 CDS ARMA_0017 2339. (go.jp)
- 3094 hypothetical protein BBZA01000002 CDS ARMA_0018 3478. (go.jp)
- 4371 hypothetical protein BBZA01000002 CDS ARMA_0019 complement(4468. (go.jp)
- 5751) hypothetical protein BBZA01000002 CDS ARMA_0021 complement(5880. (go.jp)
- 6020) hypothetical protein BBZA01000002 CDS ARMA_0020 6069. (go.jp)
- 7490 hypothetical protein BBZA01000002 CDS ARMA_0022 complement(7569. (go.jp)
- 10130) hypothetical protein BBZA01000002 CDS ARMA_0023 10363. (go.jp)
- 12985 hypothetical protein BBZA01000002 CDS ARMA_0025 13067. (go.jp)
- 13828 hypothetical protein BBZA01000002 CDS ARMA_0026 13840. (go.jp)
- 15584 hypothetical protein BBZA01000003 CDS ARMA_0028 complement(2147. (go.jp)
- 2662) hypothetical protein BBZA01000003 CDS ARMA_0030 complement(2909. (go.jp)
- 3070) hypothetical protein BBZA01000003 CDS ARMA_0029 3089. (go.jp)
- 3247 hypothetical protein BBZA01000003 CDS ARMA_0031 3817. (go.jp)
- 11054 hypothetical protein BBZA01000003 CDS ARMA_0036 11082. (go.jp)
- 11983 hypothetical protein BBZA01000003 CDS ARMA_0038 complement(11960. (go.jp)
- 12616) hypothetical protein BBZA01000003 CDS ARMA_0039 12726. (go.jp)
- 13859 hypothetical protein BBZA01000003 CDS ARMA_0040 13859. (go.jp)
- 14554 hypothetical protein BBZA01000003 CDS ARMA_0041 14582. (go.jp)
- 15289 hypothetical protein BBZA01000003 CDS ARMA_0042 15306. (go.jp)
- 15746 hypothetical protein BBZA01000003 CDS ARMA_0043 complement(15750. (go.jp)
- 16253) hypothetical protein BBZA01000003 CDS ARMA_0044 complement(16376. (go.jp)
- 17762 hypothetical protein BBZA01000003 CDS ARMA_0046 17875. (go.jp)
- 19373 hypothetical protein BBZA01000003 CDS ARMA_0048 complement(19431. (go.jp)
- 20651) hypothetical protein BBZA01000003 CDS ARMA_0049 20873. (go.jp)
- 21793 hypothetical protein BBZA01000003 CDS ARMA_0050 21812. (go.jp)
- 24549 hypothetical protein BBZA01000003 CDS ARMA_0052 24709. (go.jp)
- 25812 hypothetical protein BBZA01000003 CDS ARMA_0053 25854. (go.jp)
- 27638 hypothetical protein BBZA01000003 CDS ARMA_0054 27690. (go.jp)
- 29561 hypothetical protein BBZA01000003 CDS ARMA_0055 29551. (go.jp)
- 30963 hypothetical protein BBZA01000003 CDS ARMA_0056 30956. (go.jp)
- 32158 hypothetical protein BBZA01000003 CDS ARMA_0057 32158. (go.jp)
- 35130 hypothetical protein BBZA01000003 CDS ARMA_0058 35127. (go.jp)
- 35501 hypothetical protein BBZA01000003 CDS ARMA_0059 35523. (go.jp)
- 38051 hypothetical protein BBZA01000003 CDS ARMA_0060 38048. (go.jp)
- 38443 hypothetical protein BBZA01000003 CDS ARMA_0061 38445. (go.jp)
- 40466 hypothetical protein BBZA01000003 CDS ARMA_0063 complement(40556. (go.jp)
- 42784) hypothetical protein BBZA01000004 CDS ARMA_0066 23. (go.jp)
- 493 hypothetical protein BBZA01000004 CDS ARMA_0067 664. (go.jp)
Subunit2
- protein_coding" "Cz01g39150.t1","No alias","Chromochloris zofingiensis","RNA polymerase subunit RPB4/RPC9 [Interproscan]. (ntu.edu.sg)
- The protein is an alpha 2 dimer with subunit molecular mass of 59 kDa. (rhea-db.org)
Ubiquitin2
- Probe Set ID Ref Seq Protein ID Signal Strength Name Gene Symbol Species Function Swiss-Prot ID Amino Acid Sequence 1367452_at NP_598278 16.52 small ubiquitin-related modifier 2 precursor Sumo2 Rattus norvegicus " Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. (nih.gov)
- Regulates E3 ubiquitin-protein ligase activity of RNF19A (By similarity). (nih.gov)
Biotin4
- Targeted and proximity-dependent promiscuous protein biotinylation by a mutant Escherichia coli biotin protein ligase. (wikigenes.org)
- Song, X. Henke, S. K. and Cronan, J. E. (2021) A division of labor between two biotin protein ligase homologs. (illinois.edu)
- Following the analysis of transposon Tn5432-induced mutants of Corynebacterium glutamicum ATCC 13032, a gene encoding a protein with a biotin-binding motif was cloned. (uni-bielefeld.de)
- The protein is composed of two domains, an N-terminal biotin carboxylase and a C-terminal biotin-carboxyl-carrier protein, that are highly similar to corresponding subunits from prokaryotic and eukaryotic biotin enzymes. (uni-bielefeld.de)
Ribosomal protein3
- 23209 ribosomal protein S12 methylthiotransferase rimO BBZA01000003 CDS ARMA_0051 23344. (go.jp)
- protein_coding" "Cz03g11040.t1","No alias","Chromochloris zofingiensis","Ribosomal protein/NADH dehydrogenase domain [Interproscan]. (ntu.edu.sg)
- Ribosomal protein L11, N-terminal [Interproscan]. (ntu.edu.sg)
Synthesis1
- Respiratory electron transport, ATP synthesis by chemiosmotic coupling, and heat production by uncoupling proteins. (nih.gov)
Domain-contain1
- sigma-54 interaction domain-containing protein. (ntu.edu.sg)
Molecule2
- Search proteins in UniProtKB for this molecule. (rhea-db.org)
- SNAP- and CLIP-tag protein labeling systems enable the specific, covalent attachment of virtually any molecule to a protein of interest. (neb.com)
Genes3
- Transcription by RNA POL II is bona fide assumed for protein coding genes but must be supported by alpha-amanitin data if the end product is an RNA. (expasy.org)
- While other PD-associate genes, such as LRRK2 , SNCA , and GBA1 , regulate lysosomal functions, lipid metabolism, or protein aggregation, some have been shown to indirectly affect the electron transport chain. (frontiersin.org)
- We will then summarize some of the well-known and newly identified PD-associated genes and their direct or indirect influences on ETC. Finally, we discuss the OXPHOS-independent functions of ETC proteins and their possible implications in PD pathogenesis. (frontiersin.org)
Membrane4
- HlyD membrane-fusion protein of T1SS, Barrel-sandwich domain of CusB or HlyD membrane-fusion [Interproscan]. (ntu.edu.sg)
- The Transporter Classification Database (or TCDB ) is an International Union of Biochemistry and Molecular Biology (IUBMB)-approved classification system for membrane transport proteins , including ion channels . (wikipedia.org)
- FACL4 antibody [HL229] detects FACL4 protein at cell membrane and cytoplasm by immunohistochemical analysis. (genetex.com)
- Following the reaction, the newly synthesized acylcarnitine is transported into the mitochondrial matrix by a mitochondrial carnitine/acylcarnitine carrier protein found in the mitochondrial inner membrane. (smpdb.ca)
Amino acids1
- Many acyl-CoA groups will then further react with other zwitterionic compounds such as carnitine (to form acylcarnitines) and amino acids (to form acyl amides). (smpdb.ca)
Bacteria1
- We are in process of figuring out which proteins these bacteria use in place of those used by E. coli . (illinois.edu)
Covalent attachment1
- Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. (nih.gov)
Biosynthetic1
- Subcloning, expression, and purification of the enterobactin biosynthetic enzyme 2,3-dihydroxybenzoate-AMP ligase: demonstration of enzyme-bound (2,3-dihydroxybenzoyl)adenylate product. (rhea-db.org)
Carboxylase1
- Over 70% identity was found to a protein from Mycobacterium leprae proposed to be part of an acyl-CoA carboxylase. (uni-bielefeld.de)
Cytochrome1
- Like UQCRC1 in preventing cytochrome c from release, functions of ETC proteins beyond oxidative phosphorylation might also contribute to the pathogenesis of PD. (frontiersin.org)
Characterization1
- Structural and functional characterization of recombinant human haemoglobin as an artificial oxygen carrier. (du.ac.in)
Sequence1
- Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FACL4. (genetex.com)
Mitochondria2
- Mammalian mitochondria contain a soluble acyl carrier protein. (wikigenes.org)
- The general role of acylcarnitines is to transport acyl-groups, organic acids and fatty acids, from the cytoplasm into the mitochondria so that they can be broken down to produce energy. (smpdb.ca)
Transmembrane1
- ETC is composed of transmembrane complexes I (cI) to V (cV) and two electron carriers, the ubiquinone (i.e. (frontiersin.org)
Cytoplasm2
- The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. (nih.gov)
- FACL4 antibody [HL229] detects FACL4 protein at cytoplasm by immunofluorescent analysis. (genetex.com)
Acyltransferase1
- 601 1-acyl-sn-glycerol-3-phosphate acyltransferase plsC BBZA01000001 CDS ARMA_0002 604. (go.jp)
Residues1
- This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. (nih.gov)