Protein lamin. Medical search. Frequent questions

Expressed in differentiated cells, A-type lamins originate from the LMNA gene. (wikipedia.org)
Thus far among the lamins, these have been described only for LMNA , which maps to chromosome 1q21.2 and encodes lamin A and lamin C through alternative splicing. (bmj.com)
Recently, MAD has been shown to be caused by a specific mutation (R527H) in LMNA , which encodes lamins A and C. (bmj.com)
Lamins A and C, in contrast, arise through alternative splicing of the same primary transcript encoded by the LMNA gene, expression of which is developmentally regulated. (rupress.org)
Progerin is a mutated version of a normal cellular protein called lamin A, which is encoded by the normal LMNA gene. (sciencedaily.com)
The research suggests that the shortening of telomeres during normal cell division in individuals with normal LMNA genes somehow alters the way a normal cell processes genetic information when turning it into a protein, a process called RNA splicing. (sciencedaily.com)
Most importantly, this alteration in RNA splicing affects the processing of the LMNA messenger RNA, leading to an accumulation of the toxic progerin protein. (sciencedaily.com)
Mammalian genomes contain 3 lamin genes: LMNA, which encodes lamin A and lamin C (A-type lamins), as well as LMNB1 and LMNB2 that encode lamin B1 and B2 (B-type lamins), respectively. (aad.org)
EDMD2/EDMD3 is due to mutations (autosomal dominant and autosomal recessive, respectively) in the LMNA gene that codes for lamins A and C. Mutations in LMNA occur throughout the gene and can cause several different phenotypes (see Causes ). (medscape.com)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Lamin A/C (LMNA) in Tissue homogenates and other biological fluids. (orbitalbiosciences.com)
A CMD Subtype that results from gene defects in the lamin A/C (LMNA) gene affectings production of lamin A and lamin C prteins within the nucleus of cells. (cmdir.org)
Lamin A and lamin C are structural filament proteins in the cell nucleus, encoded by the gene LMNA . (msdmanuals.com)
and mutations in the LMNA gene encoding LAMIN TYPE A, a NUCLEAR LAMINA protein. (bvsalud.org)

This gene encodes a B type nuclear lamin. (nih.gov)
Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. (nih.gov)
Vertebrate lamins consist of two types, A and B. Mutations in this gene are associated with acquired partial lipodystrophy. (nih.gov)
Lamins have elastic and mechanosensitive properties, and can alter gene regulation in a feedback response to mechanical cues. (wikipedia.org)
Two isoforms, lamins A and C, can be created from this gene via alternative splicing. (wikipedia.org)
This nucleoplasmic lamin A environment tends to be euchromatic, suggesting distinct roles of lamin A in the regulation of gene expression in peripheral and more central regions of the nucleus. (frontiersin.org)
We discuss associated epigenetic and genome conformation changes elicited by the lamin A R482W mutation at the gene level. (frontiersin.org)
The resulting duplication of the lnp1(+) gene, which encodes an endoplasmic reticulum membrane protein, suppressed lem2∆ phenotypes, whereas the lem2∆ lnp1∆ double mutant showed a severe growth defect. (nih.gov)
This gene provides instructions for making the lamin B1 protein. (medlineplus.gov)
Through these associations, the lamins may have more expanded roles at the cellular level and control diverse functions such as DNA synthesis, gene expression, and apoptosis. (bmj.com)
Mutational analysis of the lamin A/C gene revealed homozygosity for the identical R527H mutation as reported previously, but with a distinct haplotype. (bmj.com)
Hutchinson-Gilford progeria syndrome is a rare condition caused when a mutation, which probably took place in the testes or ovaries of a child's parents, results in a single DNA letter change in one of the two copies of the gene for the lamin A protein. (newscientist.com)
People with progeria still have one healthy copy of the lamin A gene - the problem is the mutant progerin protein. (newscientist.com)
The standard form of CRISPR gene editing, which involves cutting DNA with the Cas9 protein, can be used to disable the mutant gene. (newscientist.com)
He and his colleagues have now used a CRISPR base editor to correct the single-letter change that causes almost all cases of progeria, first in skin cells taken from a person with progeria and then in mice with a human version of the lamin A gene. (newscientist.com)
In progeria, for example, the decisive damage is to a gene that makes lamin A, a protein located just under the nuclear membrane of a cell. (newscientist.com)
People with progeria have a mutated version of the gene which produces progerin, an abnormal and malfunctioning version of lamin A. This results in the symptoms of premature ageing. (newscientist.com)
Maria Eriksson of the Karolinska Institute in Sweden, who discovered the progeria mutation in 2003, also thinks it unlikely that Brooke's condition relates to progeria, or to damage to the lamin A gene. (newscientist.com)
In 5 of 6 gene mutations that have been shown to cause EDMD, the affected protein is present in the LINC (linker of nucleoskeleton and cytoskeleton) complex. (medscape.com)
EDMD1 is caused by mutations in the EMD gene on the X chromosome that codes for the nuclear envelope protein emerin. (medscape.com)
Emerin is a ubiquitous inner nuclear membraneprotein, presentin nearly all cell types, although its highest expression is in skeletal and cardiacmuscle.Emerin binds to many nuclear proteins, including several gene-regulatory proteins (eg, barrier-to-autointegration factor, germ cell-less, Btf), nesprins (proteins that act as molecular scaffolds), F-actin, and lamins. (medscape.com)
They bind to structural components (emerin, nesprin), chromatin components (histone), signal transduction molecules (protein kinase C), and several gene regulatory molecules. (medscape.com)
New mutations have been found in the synaptic nuclear envelope protein 1 ( SYNE1 ) gene and in the synaptic nuclear envelope protein 2 ( SYNE2 ) gene in a few families, also termed Nesprin-1 and Nesprin-2, respectively. (medscape.com)
methylation at certain residues of histones can regulate gene expression [ 4 ], and glycosylation is responsible for targeting substrates and changing protein half-life [ 2 ]. (hindawi.com)
Either at genomic or at proteomic level, mutations have significant impact on normal gene or protein function, and human diseases could be associated with mutations like nonsynonymous single-nucleotide variations (nsSNVs) on amino acids. (hindawi.com)
Yet how gene mutations affect protein activities through posttranslational modification sites have not been widely studied. (hindawi.com)
A CMD Subtype that results from gene defects in the laminin-alpha2 gene affecting the production of merosin protein in the muscle cell. (cmdir.org)
A wide range of nuclear proteins are involved in the spatio-temporal organization of the genome through diverse biological processes such as gene transcription and DNA replication. (bvsalud.org)
Four and a half LIM protein 1 gene mutations cause four distinct human myopathies: a comprehensive review of the clinical, histological and pathological features. (medscape.com)
Mutations in the gene encoding lamin A/C cause autosomal dominant Emery-Dreifuss muscular dystrophy. (medscape.com)

Mutations in lamin genes can result in several genetic laminopathies, which may be life-threatening. (wikipedia.org)
Studies of lamins became more popular in the 1990s when it was discovered that mutations in the genes that code for lamins can be related to muscular dystrophies, cardiomyopathies, and neuropathies. (wikipedia.org)
Mutations of LEM-domain proteins are associated with laminopathy, but their cellular functions remain unclear. (nih.gov)
A combination of mutations in Lem2 and Bqt4, which encodes a nuclear membrane protein that anchors telomeres to the nuclear membrane, caused synthetic lethality. (nih.gov)
A disparate group of seemingly unrelated diseases with different affected organ systems has been attributed to lamin A/C mutations. (bmj.com)
Novelli et al analysed lamin A/C for mutations in five consanguineous Italian families with MAD. (bmj.com)
In the last decade, a number of diseases associated with tissue degeneration and premature aging have been linked with mutations in lamin A or emerin. (portlandpress.com)
Despite highly aberrant nuclear distributions of A-type lamins and emerin in lymphoblastoid cell lines derived from patients with emerin or lamin A mutations, little or no change in chromosome location was detected. (portlandpress.com)
Lastly, mutations in the transmembrane protein 43 (TMEM43), also termed LUMA, which binds to emerin and SUN2, has also been reported to cause an EDMD phenotype in a few families. (medscape.com)
Whatever the true mechanism, the discovery of mutations in several different nuclear membrane proteins that cause similar diseases will likely eventually lead to a better understanding of nuclear membrane physiology and the pathophysiology of diseases caused by mutations in these proteins. (medscape.com)

Lamins, also known as nuclear lamins are fibrous proteins in type V intermediate filaments, providing structural function and transcriptional regulation in the cell nucleus. (wikipedia.org)
The nuclear lamina (NL) is present in all animals and is composed of type V intermediate filaments called lamins plus lamin associated proteins. (aad.org)

Increased lamin B1 levels lead to decreased expression of genes that play a variety of roles in the cell, including myelin production. (medlineplus.gov)
In mammalian somatic cells, there are four major lamins, A, B1, B2, and C. The B-type lamins are encoded by separate genes (LMNB1 and LMNB2) and, as a class, are found in the nuclei of all mammalian somatic cells. (rupress.org)
4000). ( G ) Illustration showing the number of genes whose expression changed more than twofold after 28 days of lamin A or progerin expression (I, induced. (elifesciences.org)
No significant changes were observed upon expression of lamin A. In primary and TERT+ cells, 5 and 142 genes were differentially regulated upon progerin expression, respectively. (elifesciences.org)
This positive link echoes the observation that endogenous CAB ( CHLOROPHYLL A/B-BINDING PROTEINS ) genes are repositioned from the interior to the periphery of Arabidopsis cotyledon nuclei when induced by light signaling [ 8 ]. (biomedcentral.com)
Each of our cells houses all its chromosomes inside the nucleus, as well as many specialized proteins that regulate how our genes switch on or off. (alleninstitute.org)

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. (fagusantibodies.com)
Lamins are integral structural components of the nuclear lamina hypothesised to be involved in numerous cellular processes. (bmj.com)
The major components of the nuclear lamina are the A- and B-type lamins. (rupress.org)

Chromosomal aneuploidies induced upon Lamin B2 depletion are mislocalized in the interphase nucleus. (nih.gov)
Lamins were first identified in the cell nucleus, using electron-microscopy. (wikipedia.org)
These complex structures allow nuclear lamins to perform their specialized functions in maintaining the shape of the nucleus as well as roles during mitosis and apoptosis. (wikipedia.org)
Inner nuclear membrane proteins interact with chromosomes in the nucleus and are important for chromosome activity. (nih.gov)
Treatment with S. occidentalis extracts and light induces an intense lamin A/C signal at the junction of a nuclear vacuole and the nucleus. (nih.gov)
Lamin A helps to maintain the normal structure of a cell's nucleus, the cellular repository of genetic information. (sciencedaily.com)
Loading controls can be general whole cell proteins or specific markers for subcellular fractions (e.g. nucleus, mitochondria, membrane). (novusbio.com)

The hot-spot lamin A R482W mutation causing familial partial lipodystrophy of Dunnigan-type (FPLD2), affects lamin A association with chromatin at the nuclear periphery and in the nuclear interior, and is associated with 3-dimensional (3D) rearrangements of chromatin. (frontiersin.org)
15, 16 Recently, Novelli et al categorised mandibuloacral dysplasia as a laminopathy resulting from lamin A/C mutation. (bmj.com)
In a 2007 study, NIH researchers showed that normal cells of healthy people can produce a small amount of progerin, the toxic protein, even when they do not carry the mutation. (sciencedaily.com)
The current study shows that the mutation that causes progeria strongly activates the splicing of lamin A to produce the toxic progerin protein, leading to all of the features of premature aging suffered by children with this disease. (sciencedaily.com)
The mutation activates an alternative splice site, resulting in accumulation of an abnormal lamin A-derived protein named progerin. (aad.org)
In mammals, mutation of proteins involved in the nuclear architecture (lamins and lamin associated proteins) results in diseases associated with genomic instability. (cea.fr)
Nuclear lamin A/C R482Q mutation in canadian kindreds with Dunnigan-type familial partial lipodystrophy. (medscape.com)

It wasn't until 1986 that an analysis of lamin cDNA clones across a variety of species supported that lamins belong to the intermediate filament (IF) protein family. (wikipedia.org)
This sequence is lost in later forms of IF proteins, suggesting that the structure of later intermediate filaments diverged. (wikipedia.org)
The structure of lamins is composed of three units that are common among intermediate filaments: a central α-helical rod domain containing heptad repeats surrounded by globular N and C-terminal domains. (wikipedia.org)
105:105-115) in which type III intermediate size filaments are vectorially inserted to plasma and nuclear membranes by ankyrin and lamin B, respectively. (silverchair.com)
Lamins are intermediate filament proteins comprising a major structural component of the nuclear lamina, which underlies the inner membrane of the nuclear envelope in most somatic cells. (bmj.com)
In metazoans, a complex meshwork of intermediate filament proteins constitutes the so-called lamina at the inner nuclear membrane to which chromatin regions and associated factors are anchored [ 2 ]. (biomedcentral.com)
Using Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS), we identified 42 proteins in complex with APC, including β-catenin and intermediate filament (IF) proteins lamin B1 and keratin 81. (ku.edu)

While lamin B1 is found in cells throughout the body, it appears that cells in the brain are especially sensitive to changes in lamin B1. (medlineplus.gov)

During this time period, investigations of rat liver nuclei revealed that lamins have an architectural relationship with chromatin and nuclear pores. (wikipedia.org)
Although both proteins interact with chromatin and nuclear lamins, they have distinctive biochemical and functional properties. (biorxiv.org)

Current research is being performed to develop treatment methods for the aforementioned laminopathies and to investigate the role lamins play in the aging process. (wikipedia.org)
Disorders caused by defects in the nuclear lamina associated proteins are referred to as the laminopathies. (bmj.com)

A CaaX motif is found within the unique residues in prelamin A. Due to the presence of the CaaX motif, prelamin A undergoes a series of posttranslational modifications to become mature lamin A. These steps include farnesylation of the carboxyl-terminal cysteine, endoproteolytic release of the terminal amino acids, carboxymethalation of the accessible farnesylcysteine, and removal of the final fifteen residues by a zinc metalloprotease. (wikipedia.org)
Proteolytic cleavage by the ZMPSTE24 protease removes the last 15 amino acid residues and the hydrophobic farnesyl group to produce mature lamin A, which is found both at the NL and in the nuclear interior. (aad.org)

Nuclear lamins interact with inner nuclear membrane proteins to form the nuclear lamina on the interior of the nuclear envelope. (wikipedia.org)
Mid-SUN proteins are a neglected family of conserved type III membrane proteins of ancient origin with representatives in plants, animals and fungi. (brookes.ac.uk)

Lamin B2 contributes to the proliferation of bladder cancer cells via activating the expression of cell division cycle‑associated protein 3. (nih.gov)
Lamin B2 promotes the malignant phenotype of non-small cell lung cancer cells by upregulating dimethylation of histone 3 lysine 9. (nih.gov)
Western-blot analysis showed an activation of proapoptotic factors including Fas (CD95), Fas-associated protein with death domain (FADD), caspase-8, death receptor 3 (DR3) and BID in apoptotic cells induced by metallic nickel particles. (cdc.gov)
The nuclear lamina is a thin (20 nm) yet insoluble protein meshwork that, in higher cells, lines the nucleoplasmic face of the nuclear envelope (NE). (rupress.org)
2 Protein interactions are critical to the stability, localization and activity of b-catenin, and we recently performed the first proteomic analyses of the b-catenin interactome in myeloid cells. (haematologica.org)
3 This study identified Wilms tumor protein (WT1) as a putative novel interaction partner in myeloid cells. (haematologica.org)
Campisi is exploring MHC class I chain-related protein A and B (MICA/B) ligands on senescent cells, although this is not yet a sufficiently specific test. (fightaging.org)
D ) Quantification of lamin B1 levels upon progerin or lamin A expression in primary and TERT+ cells. (elifesciences.org)
Biologists at the Allen Institute have further engineered these stem cells to tag certain proteins or cellular structures with glowing fluorescent labels. (alleninstitute.org)
The protein labeled in these cells, known as lamin B1, is a key part of the nuclear envelope. (alleninstitute.org)
Utilizing this APC-M2 pAb, we immunoprecipitated endogenous APC and its binding proteins from colon epithelial cells expressing wild-type APC. (ku.edu)
Association of lamin B1 with APC in cultured cells and human colonic tissue was verified by co-immunoprecipitation and colocalization. (ku.edu)
Myelinating Schwann cells form a myelin sheath around a single axon and express high levels of myelin-related proteins and messenger RNA (mRNA). (medscape.com)

Lamin A/C proteins have key roles in nuclear structural integrity and chromosomal stability. (mdpi.com)
These genetic interactions imply that Lem2 cooperates with the nuclear membrane protein network to regulate genome stability. (nih.gov)
Protein stability is a major regulatory principle of protein function and cellular homeostasis. (nature.com)
PTMs are involved in many protein activities and cellular processes, such as protein folding, stability, conformation, and some significant regulatory mechanisms [ 1 ]. (hindawi.com)

Proteins with quantified dynamics belong to over 10 major cellular compartments and over 200 known pathways. (nature.com)
Key proteins in mitochondria and metabolic pathways are encompassed, in addition to contractile machineries and sarcolemmal signaling proteins. (nature.com)
Whereas the different DNA repair pathways and the proteins they involve are know pretty well described, how they are regulated relative to each other in time and space remains to be deciphered. (cea.fr)

But a mystery remained: What was triggering the production of the toxic progerin protein? (sciencedaily.com)
E ) Quantification of progerin protein levels upon induction with doxycycline (±DOX) in primary and TERT+ human fibroblasts. (elifesciences.org)

There are a number of inner nuclear envelope proteins that may have roles in chromosome location and anchorage, e.g. emerin and A-type lamins. (portlandpress.com)

Emerin and LBR are abundant transmembrane proteins of the nuclear envelope (NE) that are concentrated at the inner nuclear membrane (INM). (biorxiv.org)
These included previously characterized NE-concentrated proteins, as well as a host of additional proteins not previously linked to emerin or LBR functions. (biorxiv.org)
Overall, this work presents methodology that may be used for large-scale mapping of the landscape of the INM and reveals a group of new proteins with potential functional connections to emerin and LBR. (biorxiv.org)
This complex includes nuclear membrane integral and associated proteins including emerin, lamin A/C, SUN1, SUN2, nesprin-1, and nesprin-2 that are proposed to form a mechanical link between the nucleoskeleton and cytoskeleton. (medscape.com)
Multiple and surprising new functions for emerin, a nuclear membrane protein. (medscape.com)
Ellis JA, Yates JR, Kendrick-Jones J, Brown CA. Changes at P183 of emerin weaken its protein-protein interactions resulting in X-linked Emery-Dreifuss muscular dystrophy. (medscape.com)

We further wanted to ascertain whether this protein interaction was direct using recombinant versions of purified b-catenin and WT1 protein but failed to detect any association ( Online Supplementary Figure S1B, C ). This suggested that perhaps the interaction is mediated through a post-translational modification, common partners such as WTX, 8 or cellular structures like DNA, given their well-documented roles in transcription. (haematologica.org)
Protein posttranslational modifications (PTMs) play key roles in a variety of protein activities and cellular processes. (hindawi.com)

As an aside, you might recall that mutant lamin A is the culprit in progeria , and has been postulated to be a low-level contribution to normal aging as well. (fightaging.org)

This mechanism appears to be altered by telomere shortening, and affects protein production for multiple proteins that are important for cytoskeleton integrity. (sciencedaily.com)
Progerin induced senescence, lamin B1 loss, DNA damage, and telomere shortening are prevented by TERT in primary and HGPS fibroblasts, control experiments. (elifesciences.org)

The telomeric protein AKTIP interacts with A- and B-type lamins and is involved in regulation of cellular senescence. (sens.org)
Now a large and ever increasing number of genetic subtypes has been described, and major advances in molecular and cellular biology have clarified the understanding of the role of different proteins in the physiology of peripheral nerve conduction in health and in disease. (medscape.com)

A DNA sequence encoding theHuman Lamin B1 protein (P20700) (Ser428-Ile550) was expressed with a N-His. (fagusantibodies.com)

Lamin A and beta-actin are involved in the process of apoptosis. (cdc.gov)

We show that ionizing radiation specifically enhances the motility and invasiveness of human GSCs through the stabilization and nuclear accumulation of the hypoxia-inducible factor 1α (HIF1α), which in turn transcriptionally activates the Junction-mediating and regulatory protein (JMY). (nature.com)
We show that radiation-induced migration/invasion occurs through the stabilization and nuclear accumulation of the transcription factor hypoxia-inducible factor 1 alpha (HIF1α), which drives the transcription of Junction-mediating and regulatory protein (JMY) 27 that stimulates GSC migration through its actin nucleation-promoting activity. (nature.com)
We suggest that local accumulation of lamin A in the nuclear evelope leads to bending of the structure. (fightaging.org)

Lamin A/C cardiomyopathies are genetic disorders causing dilated cardiomyopathy, a variety of arrhythmias, conduction disorders, and increased risk of sudden death. (msdmanuals.com)

This leads to the production of an abnormal protein called progerin that interferes with cell division and causes many symptoms of premature ageing. (newscientist.com)

In this work, we show that polyclonal antibodies specific for lamin B recognizes a component of the plasma membrane of Torpedo electrocyte. (silverchair.com)

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. (nih.gov)
During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. (nih.gov)
At the nuclear periphery, the genome is anchored to A- and B-type nuclear lamins in the form of heterochromatic lamina-associated domains. (frontiersin.org)
A-type lamins also associate with chromatin in the nuclear interior, away from the peripheral nuclear lamina. (frontiersin.org)
The nuclear envelope consists of an outer and inner membrane, nuclear pores and the nuclear lamina, a polymer of A-type lamins (lamin A/C, abbreviated here as lamin A) and B-type lamins (lamins B1 and B2) ( Burke and Stewart, 2013 ). (frontiersin.org)
Clearly then, A-type lamins are not strictly required for the formation of a nuclear lamina and NE. (rupress.org)
During mitosis, PP1 appears largely chromatin bound, but in telophase it is recruited to the nuclear periphery to dephosphorylate B-type lamins and thereby enable polymerization and lamina assembly. (rupress.org)
Spatial changes in nuclear lamina coincide with redistribution of lamin A proteins and local reduction in protein mobility in senescent cell. (fightaging.org)
In a recent study, Bi and colleagues identified genomic regions that are associated with the Arabidopsis NUCLEOPORIN1 (NUP1) protein, an inner subunit of the nuclear pore complex (NPC) [ 5 ] responsible for mRNA export that protrudes out into the lamina-like matrix (Fig. 1 ). (biomedcentral.com)

Here, we highlight features of nuclear lamin association with the genome at the nuclear periphery and in the nuclear interior. (frontiersin.org)
Association of A-type lamins with chromatin at the nuclear periphery and in the nuclear interior. (frontiersin.org)
B ) Immunofluorescence microscopy using V5-tag antibody (top panels) showing doxycyclin-dependent expression of v5-lamin A and v5-progerin in TERT+ human fibroblasts and localization to the nuclear periphery. (elifesciences.org)

Lamin A/C cumulative protein expression of all variants is reported by semi-quantitative Western blotting. (mdpi.com)
Using an integrated platform of metabolic labeling, high-resolution mass spectrometry and computational analysis, we report here a comprehensive dataset of the in vivo half-life of 3,228 and the expression of 8,064 cardiac proteins, quantified under healthy and hypertrophic conditions across six mouse genetic strains commonly employed in biomedical research. (nature.com)
The dataset contains over 1.92 million data points in protein isotope labeling kinetics, culminating as the in vivo turnover rates of 3,228 cardiac proteins and the expression levels of 8,064 proteins. (nature.com)
In order to idenitfy appropriate cell lines in which to study b-catenin:WT1 interplay we first performed a screen of myeloid cell lines to examine b-catenin and WT1 protein expression. (haematologica.org)
Observed changes in target protein expression can only be considered true if expression of the loading control is equal across wells. (novusbio.com)
Loading controls normalize target protein expression across all wells. (novusbio.com)
A ) Western blotting showing inducible expression of progerin or wild type lamin A in primary and TERT+ human fibroblasts ± doxycyclin (DOX) as indicated. (elifesciences.org)
The decreased expression and inhibition of nuclear transfer of GLI1 downregulated macrophage M1 polarization and osteoclast activation, the effect of which was achieved by modulation of DNA methyltransferases (DNMTs) via transcriptional regulation and protein interactions. (elifesciences.org)

A loading control is an antibody specific for a ubiquitously and constitutively expressed protein and can be used to normalize protein levels in Western blot. (novusbio.com)

It seems now clear that posttranslational modification of both DNA repair and checkpoint proteins is of importance for the regulation of their activities but how these modifications are regulated and how they affect the activity of the proteins only begins to be described. (cea.fr)
More than 200 different types of protein posttranslational modifications (PTMs) have been detected. (hindawi.com)

Unlike lamin C, Lamin A is generated in a precursor form called prelamin A. Prelamin A and lamin C differ in structure only at the carboxyl-terminus. (wikipedia.org)
The lamin A precursor also contains a CaaX motif and is initially farnesylated. (aad.org)

Abcam: antibodies, proteins, kits. (abcam.com)

(D) Nucleoplasmic lamin A interacts with chromatin in the nuclear interior. (frontiersin.org)

The history of protein dynamics traces back to 1935, when Schoenheimer and Rittenberg synthesized the first isotopologs of biological molecules to demonstrate the continuous renewal of proteins throughout life 14 , 15 . (nature.com)
In contrast to steady-state protein abundance, which may be quantified directly in mass spectrometry (MS) by spectral intensity 16 , 17 or sampling frequency 18 , 19 , protein turnover rates cannot be predicted from steady-state data 20 , requiring instead methods that can distinguish old and new protein molecules in mass spectra 11 , 21 . (nature.com)
Not ransom-note strange, but there were hand-drawn diagrams with cutouts of little cartoon weightlifters representing protein molecules. (propublica.org)

Lamin proteins are involved in the disassembling and reforming of the nuclear envelope during mitosis, the positioning of nuclear pores, and programmed cell death. (wikipedia.org)

Lamin A/C cardiomyopathies account for about 5 to 10% of idiopathic dilated cardiomyopathies. (msdmanuals.com)

Indeed, some cell types never express A-type lamins. (rupress.org)
This eventually leads to dispersal of A- and B-type lamin homooligomers throughout the mitotic cell ( Gerace and Blobel 1980 ). (rupress.org)
In telophase, the dispersed lamins are recycled to form NEs in each daughter cell. (rupress.org)
rather, the cell splices together segments of genetic information called exons that contain the code for building proteins, and removes the intervening letters of unused genetic information called introns. (sciencedaily.com)
I'm using cell and molecular biology techniques, biochemistry as well as microscopy to characterise the plant SUN proteins. (brookes.ac.uk)
This includes finding out what other proteins the SUNs bind to and what functions they have during cell division. (brookes.ac.uk)
WT1 is an RNA-binding protein, 6 and β-catenin has also been shown to bind RNA, 7 so to confirm this interaction was not indirect via RNA binding we repeated WT1 Co-IP (with or without CHIR99021) after first confirming complete digestion of RNA through RNase A pre-treatment of cell lysates ( Figure 1E ). (haematologica.org)
A) Immunoblot of myeloid leukemia cell lines showing the relative level of b-catenin (~92 kDa) and WT1 (~50 kDa) protein, with b-actin (42 kDa) used to assess protein loading. (haematologica.org)
The discovery of the ' Yamanaka factors '-four transcription factors (Oct3/4, Sox2, c-Myc and Klf4), ), proteins that can reprogram a fully mature cell into an embryonic-like state-earned Kyoto University researcher Shinya Yamanaka a share of the Nobel prize in 2012. (scientificamerican.com)
Description: A sandwich ELISA kit for detection of Lamin A/C from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (orbitalbiosciences.com)
Laminins plays a major role in binding itself and other proteins together not only in the extracellular matrix but also to the muscle cell membrane. (cmdir.org)

Here, prelamin A contains two extra exons that lamin C lacks. (wikipedia.org)

In addition, activation of antiapoptotic factors including phospho-Akt (protein kinase B) and Bcl-2 was detected. (cdc.gov)
have previously shown that reassembly of B-type lamins is under the control of both protein phosphatase 1 (PP1) and an A-kinase anchoring protein, AKAP149. (rupress.org)

The latter is a membrane protein localized to both the ER and nuclear membranes, and contains a specific binding site for PP1. (rupress.org)

While the head domain of lamins is fairly consistent, the composition of the tail domain varies based on the type of lamin. (wikipedia.org)

Anatomy4

Diseases4

Chemicals and Drugs5

Information Science1

LMNA13

Gene30

Mutations10

Type V intermediate filaments2

Genes6

Components of the nuclear lamina3

Nucleus7

Mutation7

Intermediate7

Changes in lamin1

Chromatin and nuclear2

Laminopathies2

Mature lamin2

Membrane proteins2

Cells13

Stability4

Pathways3

Progerin protein2

Chromosome1

Emerin6

Roles2

Progeria1

Telomere2

Cellular2

P207001

Apoptosis1

Accumulation3

Cardiomyopathy1

Abnormal1

Polyclonal1

Lamina9

Periphery3

Expression8

Antibody1

Posttranslational2

Precursor2

Antibodies1

Nucleoplasmic1

Molecules3

Mitosis1

Cardiomyopathies1

Cell11

Exons1

Kinase2

Nuclear membranes1

Tail domain1