Protein labeling protein detection. Medical search. Frequent questions

There is a large number of biotinylation reagents available that exploit the wide range of possible labelling methods. (genomicglossaries.com)
Fluorescein isothiocyanate (FITC) remains one of the most popular fluorescent labeling reagents, probably due to the low cost of the material. (mobitec.com)

Fluorescent characterization and X-ray photoelectron spectroscopy analysis were applied to assess the successful immobilization of the C-reactive protein (CRP) antibody. (ntu.ac.uk)
Based on Bio-Layer Interferometry (BLI), Octet ® BLI systems enable real-time, label-free analysis for the determination of kinetics, affinity and antibody/protein quantitation. (sartorius.com)
An antibody is used to precipitate a protein along with bound proteins. (genomicglossaries.com)
We took advantage of the antibody resources publicly available from the Human Protein Atlas project covering more than 80% of all human protein-coding genes. (nih.gov)
The protein of interest is then detected by a simple antigen-antibody reaction using appropriate antibodies. (bdbiosciences.com)
A primary antibody is used to bind to the specific protein of interest followed by a secondary antibody to detect the antigen-antibody complex. (bdbiosciences.com)
The primary antibody usually recognizes a specific protein or epitope and is conjugated or tagged to a fluorescent dye or an enzyme to enable subsequent detection. (bdbiosciences.com)
Fluorescence- or chemiluminescence-based detection methods are normally used for the detection of antigen-antibody complex based on the primary antibody employed in the experiment. (bdbiosciences.com)
In fluorescence-based secondary detection, fluorophore-conjugated secondary antibodies are used to bind the primary antibody and the signal is detected using a digital imager. (bdbiosciences.com)
In chemiluminescent detection, a secondary antibody conjugated to HRP is used to bind to the primary antibody. (bdbiosciences.com)
Like in western blotting, protein detection using an ELISA also uses antibody binding to antigens and could be accomplished with one antibody (direct ELISA) or using a primary and a secondary antibody (indirect ELISA). (bdbiosciences.com)
Zirconia nanoparticles (ZrO2 NPs) were used as selective sorbents to capture the phosphorylated AChE adduct, and quantum dots (ZnS@CdS, QDs) were used as tags to label monoclonal anti-AChE antibody to track the immunorecognition events. (cdc.gov)

Absolute quantification with mass spectrometry and isotope labelled internal standards has found broad applications in biomedical research. (nist.gov)
Here, we describe a new method (immuno-SILAC) for the absolute quantification of proteins in complex samples based on polyclonal antibodies and stable isotope-labeled recombinant protein fragments to allow affinity enrichment prior to mass spectrometry analysis and accurate quantification. (nih.gov)
This methodology enables detection of putative metabolites from biological samples and simultaneous quantification of the pattern and extent of isotope labeling. (acs.org)
The methodology involves combining Molecular Biology (DNA Nanoball generation) and electronics (electric impedance-based quantification) to yield a pioneering detection tool , says Vicent Pelechano. (nanotech-now.com)
By combining the results from two-stage quantification, 4 or 5 orders of magnitude in dynamic range were achieved for the detection of the serotypes of BoNT/A, /B, /E, or /F. The effect of multiplexing the assay by mixing substrates for different BoNT serotypes into a single reaction was also investigated in order to reduce the numbers of the cleavage reactions and to save valuable clinical samples. (cdc.gov)
In addition, the effectiveness of antitoxin treatment may vary depending on circulating toxin levels, so that rapid identification and quantification of BoNT proteins in clinical specimens and food is desirable for optimization of treatment. (cdc.gov)

Protein labeling kits from AnaSpec include Biotin, fluorescent dye, fluorescent protein, TR-FRET: all together twenty one AnaTag™labeling kits. (anaspec.com)
Protein found in raw egg white which binds to biotin (etymology is from its avidity to biotin). (genomicglossaries.com)
The interaction of biotin and Avidin or Streptavidin has been exploited for use in many protein and nucleic acid detection and purification methods. (genomicglossaries.com)
Because the biotin label is stable and small, it rarely interferes with the function of labeled molecules enabling the Avidin-biotin interaction to be used for the development of robust and highly sensitive assays. (genomicglossaries.com)
Stable, high-affinity streptavidin monomer for protein labeling and monovalent biotin detection. (addgene.org)

With a wide range of ligand capture surfaces that allow for immobilization of antibodies, recombinant proteins, DNA, RNA and virus-like particles, the Octet ® BLI platform has become a must-have laboratory tool for biomolecular interactions studies. (sartorius.com)
Epitope mapping revealed that a majority of the polyclonal antibodies recognized multiple linear epitopes, and based on these results, a semi-automated method was developed for peptide enrichment using polyclonal antibodies immobilized on protein A-coated magnetic beads. (nih.gov)
A protocol based on the simultaneous multiplex capture of more than 40 protein targets showed that approximately half of the antibodies enriched at least one functional peptide detected in the subsequent mass spectrometry analysis. (nih.gov)
Here, we show that we were able to use small amounts of antibodies (50 ng per target) in this manner for efficient multiplex analysis of quantitative levels of proteins in a human HeLa cell lysate. (nih.gov)
The results suggest that polyclonal antibodies generated via immunization of recombinant protein fragments could be used for the enrichment of target peptides to allow for rapid mass spectrometry analysis taking advantage of a substantial reduction in sample complexity. (nih.gov)
The antibodies are specific against mouse CD45 and MHC class I (of a, b, d, j, k, s, and u haplotypes) and can be used to label hematopoietic and non-hematopoietic cells in most commonly used mouse strains for multiplex single cell sequencing analysis. (biolegend.com)
Polyclonal antibodies are comparatively less specific but offer broader recognition of the antigen and provide higher chances of obtaining qualitative information about proteins. (bdbiosciences.com)
Depending on the method, secondary detection is carried out using antibodies conjugated to enzymes or fluorophores. (bdbiosciences.com)
Since fluorophores with absorption and emission maxima spanning the entire light spectrum are available, combinations of antibodies conjugated to fluorophores of different wavelengths can be used to detect multiple proteins. (bdbiosciences.com)
Labeled antibodies against several important biological target proteins are commercially available. (bdbiosciences.com)
Secondary antibodies facilitate signal detection and amplification. (bdbiosciences.com)
Sera from residual specimens from NHANES 2001-2002 participants were tested for levels of IgG antibodies against cancer antigen (CA) 125, CA15.3 and human epididymal secretory protein 4 (HE4). (cdc.gov)
Module B contained human serum samples for the detection of anti-DENV antibodies. (who.int)

We developed genomic locus proteomics (GLoPro), in which we combine CRISPR-based genome targeting, proximity labeling, and quantitative proteomics to discover proteins associated with a specific genomic locus in native cellular contexts. (nature.com)
The combination of immuno-based methods and mass spectrometry detection has great potential in the field of quantitative proteomics. (nih.gov)

Today, several proteomics approaches (such as chemical, quantitative and functional proteomics, including the analyses of protein expression profiles, protein interaction networks and post-translational modifications) are widely carried out by clinicians and pharmaceutical companies. (mdpi.com)

We describe the design and synthesis of a novel set of iso-baric tags for quantitative glycan profiling, which will have broad applications in carbohydrate based biomarker dis-covery, therapeutic protein characterization, and vaccine development. (nist.gov)
Labeling of Trypanosoma brucei cell cultures with 50% uniformly 13C-labeled glucose demonstrated incorporation of glucose-derived carbon into 187 of 588 putatively identified metabolites in diverse pathways including carbohydrate, nucleotide, lipid, and amino acid metabolism. (acs.org)

This work addresses contaminant detection both in clinical situations and during protein purification of pharmaceutical products. (lu.se)

The researchers combined gel-permeation high-performance liquid chromatography with fluorescence detection to develop a sensitive technique that can detect both gliadins and glutenins in purified wheat starch. (healthnewsground.com)
Article: Improved Quantitation of Gluten in Wheat Starch for Celiac Disease Patients by Gel-Permeation High-Performance Liquid Chromatography with Fluorescence Detection (GP-HPLC-FLD), Katharina Anne Scherf, Herbert Wieser, and Peter Koehler, Journal of Agricultural and Food Chemistry , doi: 10.1021/acs.jafc.6b02512, published online 16 September 2016. (healthnewsground.com)
Fluorescence-tags, commonly used to visualize the spatial distribution of proteins within cells, can influence the localization of the tagged proteins by affecting their stability, interaction with other proteins or the induction of oligomerization artifacts. (nature.com)
Using yellow fluorescence protein (YFP) tagged MreB, it was initially suggested that MreB forms a continuous, cell-spanning helical filament running along the inner membrane of the cell 4 . (nature.com)
Enzymatic cleavage assays represent an attractive approach where the functional active toxins are detected by monitoring the cleavage of their target substrates, or peptide mimics in most cases, by means of different techniques, such as immunological detection 18 , 19 , capillary electrophoresis 20 , fluorescence analysis 21 , Forster resonance energy transfer (ALISSA) 22 , and high performance liquid chromatography 23 . (cdc.gov)

In addition to these qualitative aspects, western blotting can also be used for relative quantitation of proteins based on the band intensity between experimental and control samples. (bdbiosciences.com)

One way to achieve this goal is the detection of proteins by immunofluorescence. (nature.com)
To circumvent this problem, we have developed a non-invasive protocol enabling the three-dimensional (3D-) visualization of proteins in serial sections of bacterial cells by immunofluorescence microscopy or immunogold labeling envisioned by electron microscopy. (nature.com)

This 3D capacitive biosensor with integrated microfluidics is mass-producible and has achieved the on-site continuous detection of cardiac- and periodontitis-related biomarkers with high performance. (ntu.ac.uk)
Combining these new datasets with existing published gene expression and protein expression data have identified selected panels of novel biomarkers which have been evaluated on over 100 cervical cancer and pre-cancer cases using standard immunohistochemistry approaches. (europa.eu)
Specifically the development of an electrochemical immunosensor for determination of phosphorylated cholinesterase (OP-ChE) adduct biomarkers using quantum dot (QD) labels. (cdc.gov)

The approach was further developed to also generate quantitative data via the addition of heavy isotope-labeled recombinant protein fragment standards prior to trypsin digestion. (nih.gov)
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. (rndsystems.com)
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (rndsystems.com)

Intriguingly, helical localization patterns were reported for many other fluorescently tagged bacterial proteins (e.g. chemoreceptors, secretion proteins, RNase E, and the chromosome partitioning protein SetB) but often the questions remains how well these observations reflect the true situation 6 . (nature.com)
However, at present no ssEM protocol was available to investigate protein localization in entire bacterial cells. (nature.com)
indicating their potential role as an anchor for these proteins to regulate their localization and dynamics during synaptic transmission. (jneurosci.org)
Using western blotting, proteins can be characterized for several aspects, including their presence or absence, abundance, phosphorylation status or localization. (bdbiosciences.com)

Low limit of detection and minimal non-specific binding show potential for in vitro and in vivo assays. (rsc.org)

A synthetic, specially- designed oligonucleotide with the ability to recognize and bind a protein ligand molecule or molecules with high affinity and specificity. (genomicglossaries.com)

With unparalleled ease-of-use, the instruments transform workflows beyond the limitations of label-free methods, providing comprehensive characterization capabilities across a broad range of applications critical for efficient biotherapeutics development and production. (sartorius.com)
Therefore, label-free methods are critically required in order to validate findings obtained by this commonly used method. (nature.com)
Here, we propose to tackle this challenge using anomaly detection methods that automatically identify unexpected properties. (biorxiv.org)
ELISAs also use colorimetric or enzyme-based methods for detection. (bdbiosciences.com)
Detection of acute/recent DENV infection by both molecular (RT-PCR) and serological methods (IgM) was available in 19/24 (79.2%) participating laboratories. (who.int)

The limit of detection of the 3D capacitive biosensor for CRP label-free detection was about 1 pg mL−1. (ntu.ac.uk)
development and testing of an integrated DNA protein copying technology with label free biosensor detection technology. (europa.eu)
Labib, M., Hedström, M. , Amin, M., Mattiasson, B. A novel competitive capacitive glucose biosensor based on concanavalin A-labeled nano-gold colloids assembled on a polytyramine-modified gold electrode. (lu.se)

Labeling patterns confirmed the metabolic pathways responsible for the biosynthesis of many detected metabolites, and labeling was detected in unexpected metabolites, including two higher sugar phosphates annotated as octulose phosphate and nonulose phosphate. (acs.org)

To detect sub-clinical exposures, sensor development is focused on the detection of adducts on target proteins. (cdc.gov)
Among aquatic organisms, fish share many target proteins for human medicines. (janusinfo.se)

Botulinum neurotoxins (BoNTs) are bacterial proteins that cause the life-threatening disease botulism 1 . (cdc.gov)

Now scientists, reporting in ACS' Journal of Agricultural and Food Chemistry , have developed a more reliable way for manufacturers to detect gluten in purified wheat starch, a common ingredient in foods labeled gluten-free. (healthnewsground.com)
Calleja said that Mecwins' system is capable of detecting the bending of hundreds of microcantilevers in a "one-shot measurement," and is sensitive enough to detect DNA hybridization without the need for labels or prior PCR amplification. (genomeweb.com)
In addition to the TB assay development project, Calleja said that the researchers are also interested in applying the technology to detect gene mutations related to cancer, "as the technique has a high sensitivity, and not relying on a prior PCR or a labeling step would speed up the detection and lower the cost. (genomeweb.com)
We apply these protein language models (pLM) to detect anomalies in function, phylogenetic families, and segmentation tasks. (biorxiv.org)
Researchers at Karolinska Institute have developed a novel method using DNA Nanoballs to detect pathogens, aiming to simplify nucleic acid testing and revolutionize pathogen detection. (nanotech-now.com)
Western blotting-or immunoblotting-is a technique used to detect, characterize and quantitate proteins. (bdbiosciences.com)
The techniques they developed during the analysis included the discovery of sensitive "marker proteins" that can be used to detect trace amounts of nuts in processed foods. (news-medical.net)

co-immunoprecipitation Used to determine protein- protein interactions . (genomicglossaries.com)

However, the isomers may differ in their binding and reactivity to proteins, and the conjugates may elute under different chromatographic conditions or migrate differently in electrophoretic gels. (mobitec.com)

The enzyme-linked immunosorbent assay (ELISA) is another type of immunoassay widely used for protein analysis. (bdbiosciences.com)
The standard experiment is to separate the proteins, e.g. by 2D gel electrophoresis, digest the proteins with a highly specific enzyme, measure the masses of the peptide fragments with a mass spectrometer (typically a MALDI-TOF mass spectrometer) and then compare the peptide monoisotopic masses with expected monoisotopic masses from a database (protein or DNA database). (lu.se)

The success of a PMF experiment depends on several factors: The noise level in the spectrum, the mass accuracy, the amount and purity of the sample, the number of proteins in the sample, possible post-translational modifications, algorithm accuracy, and (to a considerable extent) operator skill and experience. (lu.se)

the creation of a large number of compounds (usually proteins or peptides) through technologies such as phage display . (genomicglossaries.com)
Here we discuss two approaches currently used to investigate the global dynamics of phosphorylation based on the isolation of phosphorylated proteins or peptides. (acs.org)

To circumvent these obstacles, a protocol was developed to generate 50 nm thick serial sections suitable for immunogold labeling and subsequent reconstruction of the spatial distribution of immuno-labeled native proteins within individual bacterial cells. (nature.com)
It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. (biolegend.com)
MHC class I is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. (biolegend.com)
S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. (cancerindex.org)
These toxins inhibit the release of neurotransmitters at the neuromuscular junction by cleaving soluble NSF attachment protein receptor (SNARE) complex proteins in nerve cells, which prevents the fusion of neurotransmitter containing vesicles to cell membranes 2 . (cdc.gov)
A further 20 blocks from The ISH results were given percentage vanced stages with a likely prevalence samples from normal breast tissues scores based on positive signals and of more aggressive tumour forms were labelled as a control group (i.e. number of cells that gave these signals. (who.int)

From IgG titer to epitope binning, the Octet ® RH16 system offers label-free biomolecular interaction analysis with state-of-the-art high throughput platforms that facilitate the rapid characterization and development of biologics drug molecules. (sartorius.com)
Phosphatidylinositol-4,5-bisphosphate (PI(4,5)P 2 ) plays an essential role in neuronal activities through interaction with various proteins involved in signaling at membranes. (jneurosci.org)

Regulation of gene expression is primarily controlled by changes in the proteins that occupy genes' regulatory elements. (nature.com)
Many advances in biomedicine can be attributed to identifying unusual proteins and genes. (biorxiv.org)

The bilayers are labelled with a rhodamine phospholipid (red). (lu.se)

Module A contained serum samples spiked with cultured dengue virus (DENV) or chikungunya virus (CHIKV) for the detection of nucleic acid and DENV non-structural protein 1 (NS1) antigen. (who.int)

This is key to early detection of targets. (genomeweb.com)

This is unsatisfying since strong spectra are often produced by high abundant proteins, which typically represent the bulk of the cell activity. (lu.se)

Factors that might affect the linearity and dynamic range for detection of BoNT cleavage products were initially examined, including the amount of peptide substrate and internal standard, the timing of cleavage reaction, and the components in the reaction solution. (cdc.gov)
The light chain is a zinc-metalloprotease which is responsible for the cleavage of three SNARE complex proteins including SNAP-25 (Synaptosome-associated protein of 25 kDa), synaptobrevin-2 (also termed VAMP-2) and syntaxin. (cdc.gov)

Applying this method, we show a cellular distribution of the staphylococcal alkaline shock protein 23 (Asp23), which is compatible with filament formation, a property of Asp23 that we also demonstrate in vitro . (nature.com)
In this study, we established a method for visualizing PI(4,5)P 2 by SDS-digested freeze-fracture replica labeling (SDS-FRL) to investigate the quantitative nanoscale distribution of PI(4,5)P 2 in cryo-fixed brain. (jneurosci.org)
SIGNIFICANCE STATEMENT In this study, we established an electron microscopic method to visualize and analyze the quantitative distribution pattern of phosphatidylinositol-4,5-bisphosphate (PI(4,5)P 2 ) on cell membranes using cryo-fixed brain tissues and SDS-digested freeze-fracture replica labeling. (jneurosci.org)
We conclude that the combination of pLM and anomaly detection techniques is a valid method for discovering a range of global and local protein characteristics. (biorxiv.org)
This new method, offering label-free detection could hasten the rollout of new diagnostic kits. (nanotech-now.com)
This method either uses autoradiography or a digital imager for signal detection. (bdbiosciences.com)
Mass spectrometry combined with database searching is today the preferred method for protein identification. (lu.se)
The detection limit (DL, LOD, LLD) for a chemical analysis method is the lowest concentration at which a substance can be detected. (janusinfo.se)
In this publication we report on the development of a quantitative enzymatic method for the detection of four botulinum neurotoxin (BoNT) serotypes responsible for human botulism by MALDI-TOF mass spectrometry. (cdc.gov)
2.17 ISO 6579, microbiology of food and animal feeding stuffs - horizontal method for the detection of salmonella spp. (who.int)
2.18 ISO 7251, Microbiology of food and animal feeding stuffs - horizontal method for the detection and enumeration of presumptive Escherichia coli - most probable number technique. (who.int)

The strategy therefore promises reliable automated user- independent protein identification using peptide mass fingerprints. (lu.se)
The focal point is further set at detection principles utilizing biosensors and mass spectrometry (MS). During the recent years, my research has been directed to develop process monitoring tools to be utilized in clinical situations and pharmaceutical manufacturing. (lu.se)

We demonstrate a strategy for label-free protein detection through monitoring the Surface Enhanced Raman Spectrum of an aptamer probe attached to a gold nanoshell . (rsc.org)

When people with celiac disease, wheat allergies or gluten sensitivity consume these proteins, they can experience a range of symptoms from diarrhea and vomiting to fatigue and migraines. (healthnewsground.com)
High detection sensitivity, throughput, and 384-microwell plate compatibility of the Octet ® RH16 system have also made it a favorite for small molecule library screening and analysis of large cross-competition binding campaigns. (sartorius.com)
Electrochemical immunoassay platform for high sensitivity protein detection based on redox-modified carbon nanotube labels. (bvsalud.org)

However, the distribution pattern of PI(4,5)P 2 and the association with these proteins on the neuronal cell membranes remain elusive. (jneurosci.org)

The team commenced this work as an extension of their previous endeavors in LAMP (Loop-Mediated Isothermal Amplification) based detection of SARS-CoV-2 during the pandemic. (nanotech-now.com)
2019) Ultrasensitive amplification-free detection of protein kinase based on catalyzed assembly and enumeration of gold nanoparticles. (jenabioscience.com)

The Octet ® platform's rapid high throughput protein analysis combined with the anti-CHO HCP and Residual Protein A kits provide biologics developers with a fast and easy-to-use assay for contaminant testing. (sartorius.com)

The CLP (classification, labelling and packaging) Regulation contains rules for the classification, labeling and packaging of chemical products. (janusinfo.se)

The voltammetric response of the immunosensor is highly linear over the range of 10 pM to 10 nM paraoxon-AChE, and the limit of detection is estimated to be 8 pM. (cdc.gov)

FITC-protein conjugates are suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications. (mobitec.com)

But knowing for sure whether products marketed as such are truly safe to eat can be more complicated than just reading a label. (healthnewsground.com)
The U.S. Food and Drug Administration and the United Nations' Food and Agriculture Organization have set the maximum limit for gluten in products labeled gluten-free at 20 mg/kg. (healthnewsground.com)

We generate meaningful representations without labeled inputs, using pretrained deep neural network models. (biorxiv.org)

Moreover, PRIN2 and CSP41b form a distinct protein complex in vitro that binds DNA. (frontiersin.org)

We compute protein anomaly scores to highlight human prion-like proteins, distinguish viral proteins from their host proteome, and mark non-classical ion/metal binding proteins and enzymes. (biorxiv.org)
In previous studies, the team identified specific proteins relevant to human allergies: almond major protein (AMP), cashew major protein (CMP) and walnut glutelin (WG). (news-medical.net)
The described multiple- scale strategy both avoids time-consuming parameter tuning and exceeds the human operator in protein identification efficiency. (lu.se)
The typical situation in a laboratory today is to have human operators double-check the software results and the protein identities [15]. (lu.se)

5-FITC is more widely used than 6-FITC isomer for protein labeling. (mobitec.com)

Anatomy4

Organisms3

Diseases2

Chemicals and Drugs29

Analytical, Diagnostic and Therapeutic Techniques and Equipment64

Psychiatry and Psychology2

Phenomena and Processes9

Disciplines and Occupations9

Technology, Industry, Agriculture3

Information Science7

Health Care11

Reagents2

Antibody12

Quantification6

Biotin5

Antibodies13

Quantitative proteomics2

Proteomics1

Carbohydrate2

Purification1

Fluorescence5

Quantitation1

Immunofluorescence2

Biomarkers3

Recombinant protein3

Localization4

Assays1

Affinity1

Methods5

Biosensor3

Biosynthesis1

Target proteins2

Bacterial proteins1

Detect7

Interactions1

REACTIVITY1

ENZYME2

Post-translational1

Peptides2

Cells6

Interaction2

Genes2

Rhodamine1

Antigen1

TARGETS1

Abundant1

Cleavage2

Method11

Mass2

Aptamer1

Sensitivity3

Neuronal1

Amplification2

Analysis1

Regulation1

Limit of detect1

Suitable1

Products2

Generate1

Vitro1

Human4

Widely1