Protein kinase and protein phosphorylation. Medical search. Frequent questions

In addition, markers of mitogen-activated protein kinase (MAPK), phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) and mammalian target of rapamycin pathway activation (phosphorylation of extracellular signal-regulated kinase [ERK], AKT, and p70 ribosomal protein S6 kinase β-1 [p70S6K]) were studied using standardized immunohistochemistry. (medscape.com)
Tau tubulin kinase 2 (TTBK2) is a key player in the cilium assembly pathway, controlling the final step of cilia initiation. (muni.cz)
The phosphorylation of Akt is mediated by the PI3 kinase pathway while that of CREB is dependent on the activation of both MAPK kinase and p38 MAPK. (biomedcentral.com)
Osmotic stress induces the phosphorylation of WNK4 Ser575 via the p38MAPK-MK pathway. (nih.gov)
CD40 signaling pathway: anti-CD40 monoclonal antibody induces rapid dephosphorylation and phosphorylation of tyrosine-phosphorylated proteins including protein tyrosine kinase Lyn, Fyn, and Syk and the appearance of a 28-kD tyrosine phosphorylated protein. (silverchair.com)
Time course studies suggest that phosphorylation and association of SHC with v-Abl is insufficient to lead to significant activation of MAP kinase and that activation of the MAP kinase kinase/MAP kinase pathway is not required for apoptotic suppression. (hud.ac.uk)
On the basis of immunoprecipitation experiments, in vitro kinase assay and RNA interference, we demonstrate that VEGF-A induces the c-ABL kinase activity through the VEGF Receptor-2 /Phosphatidylinositol-3-Kinase pathway. (unisi.it)
A vast number of studies have focused on understanding the nitric oxide (NO) signaling pathway, which culminates with the phosphorylation of the transcription factor cAMP-responsive element-binding protein (CREB) through the increase of the second messenger cyclic guanosine monophosphate (cGMP) and activation of cGMP-dependent protein kinase. (intechopen.com)
This book chapter provides an overview of the progress being made in modulating the hippocampal synaptic transmissions, which are critical for learning and memory, by targeting the different components of the NO/cGMP/CREB phosphorylation signaling pathway. (intechopen.com)
The CHEK2 kinase (Chk2 in mouse) is a member of a DNA damage response pathway that regulates cell cycle arrest at cell cycle checkpoints and facilitates the repair of dsDNA breaks by a recombination-mediated mechanism. (nih.gov)
The Legionella Effector Kinase LegK7 Hijacks the Host Hippo Pathway to Promote Infection. (nih.gov)
Activated RTKs initiate phosphorylation cascades that are critical for the MAPK pathway. (nih.gov)

Survival assays with various kinase inhibitors suggested that the activation/phosphorylation of both Akt and CREB contributes to IGF-1 mediated cell survival in PC12 cells. (biomedcentral.com)
They were also blocked by protein tyrosine kinase (PTK) inhibitors, herbimycin A and genistein, and PKC and protein serine/threonine kinase inhibitors, H7 and HA1004. (silverchair.com)
In addition, we have an interest in re-purposing PDE1 inhibitors for pulmonary arterial hypertension.Current research focuses on the role of G protein-coupled receptors, in particular GPR75, which we believe could be a novel target and possible circulating biomarker for pulmonary arterial hypertension. (abdn.ac.uk)
SWCNT induced a significant activation of activator protein-1 (AP-1) and nuclear factor kappa B (NF-kB), and the effect was inhibited by mitogen-activated protein kinase (MAPK) inhibitors. (cdc.gov)
He is now developing novel protein kinases inhibitors that selectively target essential parasite kinases with the aim of generating next generation anti-malarial's. (gla.ac.uk)

Several extracellular proteins have been reported to be phosphorylated. (molcells.org)
Moreover, it has been reported that protein kinase C (PKC), although known to be intracellular, can phosphorylate extracellular proteins in the case of cellular damage and/or platelet activation. (molcells.org)

The protein encoded by this gene is a member of the mitogen-activated protein kinase (MAPK) family. (nih.gov)
These family members function in a protein kinase signal transduction cascade, where an activated MAPK kinase kinase (MAP3K) phosphorylates and activates a specific MAPK kinase (MAP2K), which then activates a specific MAPK. (nih.gov)
3. PKD, PKD2, and p38 MAPK mediate Hsp27 serine-82 phosphorylation induced by neurotensin in pancreatic cancer PANC-1 cells. (nih.gov)
Additionally, IQGAP1 scaffolds downstream signaling components, such as the mitogen-activated protein kinase (MAPK) and Akt pathways. (nih.gov)

IGF-1, in a concentration- and time-dependent manner, induces the activation/phosphorylation of Akt and CREB in PC12 cells by activating different signaling pathways. (biomedcentral.com)
1. Neurotensin induces protein kinase C-dependent protein kinase D activation and DNA synthesis in human pancreatic carcinoma cell line PANC-1. (nih.gov)

Activation of CB1 receptors, either by an agonist or by inhibition of reuptake of endogenous cannabinoids, stimulates phosphorylation at Thr34, thereby converting DARPP-32 into an inhibitor of protein phosphatase-1. (jneurosci.org)
p21CIP1 is a potent, tight-binding inhibitor of Cdks and can inhibit the phosphorylation of Rb by cyclin A-Cdk2, cyclin E-Cdk2, cyclin D1-Cdk4, and cyclin D2-Cdk4 complexes. (nih.gov)
The changes in the state of tyrosine phosphorylation induced by the anti-CD40 mAb were obviated by mAb to CD45, a protein tyrosine phosphatase (PTP) or by the addition of sodium orthovanadate, a broad PTP inhibitor. (silverchair.com)
This effect was fully blocked by either the N-methyl-D-aspartate receptor antagonist D(-)-2-amino-5-phosphonopentanoic acid (APV) or the CaM kinase II inhibitor KN-62. (ox.ac.uk)
By treating HUVEC with the specific tyrosine kinase inhibitor STI571 and over-expressing a dominant negative c-ABL mutant, we show that the VEGF-A-activated c-ABL reduces the amplitude of Mitogen-Activated Protein Kinases (ERK1/2, JNKs and p38) activation in a dose-dependent manner by a negative feedback mechanism. (unisi.it)
The next step is the initiation of a chemical design and synthesis project to modify a non-specific inhibitor of the wild-type enzyme with substituents which specifically complement the mutation introduced into the active site of the protein of interest. (berkeley.edu)
A pyrazolopyrimidine based kinase inhibitor we have identified satisfies the criteria for an inhibitor which only inhibits mutated kinases and does not inhibit any wild-type kinases we have assayed. (berkeley.edu)

In the biopsies, insulin receptor kinase (IRK) activity, insulin receptor substrate (IRS)-l-associated phosphatidylinositol 3-kinase (PI3K) activity, Ser 473 and Thr 308 phosphorylation of protein kinase B (PKB), and protein expression of IRS-1, IRS-2, phosphoinositol-dependent kinase-1 (PDK-1), PKB, and GLUT-4 were determined. (uni-luebeck.de)
In the biopsies, insulin receptor kinase (IRK) activity, insulin receptor substrate (IRS)-l-associated phosphatidylinositol 3-kinase (PI3K) activity, Ser473 and Thr308 phosphorylation of protein kinase B (PKB), and protein expression of IRS-1, IRS-2, phosphoinositol-dependent kinase-1 (PDK-1), PKB, and GLUT-4 were determined. (uni-luebeck.de)

Because both E-cadherin and PKD1 are known to be dysregulated in prostate cancer, our study identified an important protein-protein interaction influencing the signal transduction system associated with cell adhesion in prostate cancer. (ox.ac.uk)

The early events triggered by CD40 were examined by monitoring the changes in tyrosine phosphorylation of cellular proteins with anti-phosphotyrosine mAb. (silverchair.com)
The tyrosine phosphorylation of this protein persisted. (silverchair.com)
The patterns of protein tyrosine phosphorylation differed from those induced by an anti-immunoglobulin M mAb. (silverchair.com)
In addition, the alteration in the tyrosine phosphorylation of PTKs Lyn, Fyn, and Syk was directly demonstrated. (silverchair.com)
Engagement of CD40 for 30 s induced a transient decrease in tyrosine phosphorylation of these PTKs. (silverchair.com)
Both IL-3 and v-Abl stimulated the tyrosine phosphorylation of SHC and GTPase-activating protein. (hud.ac.uk)
However, while IL-3 stimulated similar levels of tyrosine phosphorylation in p46(shc) and p52(shc), v-Abl preferentially phosphorylated p52(shc), an event that occurred within 1 h of temperature switch. (hud.ac.uk)

Scholars@Duke publication: Phosphorylation of FEZ1 by Microtubule Affinity Regulating Kinases regulates its function in presynaptic protein trafficking. (duke.edu)
Phosphorylation of FEZ1 by Microtubule Affinity Regulating Kinases regulates its function in presynaptic protein trafficking. (duke.edu)
Taken together, these results suggest that oxidative stress regulates the phosphorylation status of nonribosomal rpS3 by both activating PKCδ and blocking the PP2A interaction with rpS3. (korea.ac.kr)
MIR-1265 regulates cellular proliferation and apoptosis by targeting calcium binding protein 39 in gastric cancer and, thereby, impairing oncogenic autophagy. (nih.gov)

In summary, our work provides important insight into the substrates-TTBK2 kinase relationship and suggests that phosphorylation of substrates on multiple sites by TTBK2 is probably involved in the control of ciliogenesis in human cells. (muni.cz)
The discovery that centromeric Rec8 is protected from separase during meiosis I by shugoshin/MEI-S332 proteins that bind PP2A phosphatase suggests that phosphorylation either of separase or cohesin may be necessary for Rec8 cleavage. (nih.gov)

We show here that multiple phosphorylation sites within Rec8 as well as two different kinases, casein kinase 1delta/epsilon (CK1delta/epsilon) and Dbf4-dependent Cdc7 kinase (DDK), are required for Rec8 cleavage and meiosis I nuclear division. (nih.gov)

001). Patients with right-sided primary tumor and low p70S6K phosphorylation had responses to anti-epidermal growth factor receptor antibody-based therapies and overall survival similar to patients with left-sided primary tumors. (medscape.com)
The nuclear transcription factor cAMP response element-binding protein (CREB) may also be involved although relationships between these two proteins in IGF-1 receptor signaling and protection is not clear, especially in neuronal cells. (biomedcentral.com)
Binding of IGF-1 to its receptor causes receptor autophosphorylation and the activation of intrinsic tyrosine kinase. (biomedcentral.com)
Background Deleted in Malignant Brain Tumors 1 (DMBT1) is usually a secreted scavenger receptor cysteine-rich protein that binds various bacteria and is thought to participate in innate pulmonary host defense. (casein-kinase.info)
This Non-receptor tyrosine-protein kinase plays an important role in the regulation of cell growth, differentiation, migration and immune response. (wikipedia.org)
To inhibit SFKs, CSK is then recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane and ultimately suppresses signaling through various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several effector molecules. (wikipedia.org)
SWOPE, SHERIDANL, QU, ZHICAN & HUGANIR, RICHARDL 1995, ' Phosphorylation of the Nicotinic Acetylcholine Receptor by Protein Tyrosine Kinases ', Annals of the New York Academy of Sciences , vol. 757, no. 1, pp. 197-214. (johnshopkins.edu)
Troglitazone did not alter insulin receptor number or IRS-1, IRS-2, PKB, PDK-1, or GLUT-4 protein expression. (uni-luebeck.de)
10. Stimulation of the neurokinin 3 receptor activates protein kinase C epsilon and protein kinase D in enteric neurons. (nih.gov)
15. G protein-coupled receptor signaling in human ductal pancreatic cancer cells: neurotensin responsiveness and mitogenic stimulation. (nih.gov)
The first is our long-standing interest in G protein coupled receptor (GPCR) research which has spanned more than two decades. (gla.ac.uk)
These proteins are called receptors and although many drugs currently on the market, such as anti-histamines for allergies and beta-blockers for heart disease, target these receptor proteins, largely drug discovery efforts in this area fail. (gla.ac.uk)
In part, this is accomplished via interactions between IQGAP1 and a variety of receptors, including receptor tyrosine kinases (RTKs), G-protein coupled receptors (GPCRs) and chemokine receptors. (nih.gov)

Our data indicate that a considerable proportion of the psychomotor effect of cannabinoids can be accounted for by a signaling cascade in striatal projection neurons involving PKA-dependent phosphorylation of DARPP-32, achieved via modulation of dopamine D 2 and adenosine A 2A transmission. (jneurosci.org)
The neurons displayed a single class of phorbol 12,13-dibutyrate binding sites with a maximum binding capacity, after 10 d in culture, of 18.6 pmol/mg protein and an apparent dissociation constant of 7.1 nM. (uky.edu)
The apparent induction of phorbol ester receptors was specific relative to other cellular proteins and could be inhibited by cycloheximide or Actinomycin D. Phosphorylation of endogenous substrates in intact cultured neurons paralleled the age-dependent increase in protein kinase C. Furthermore, 32 P incorporation into several major phosphoproteins was markedly augmented by treating the neuronal cultures with phorbol esters. (uky.edu)
Such phosphorylation events may provide a clue to the significance of protein kinase C in developing neurons. (uky.edu)

In vitro kinase assays have shown that PKD1 phosphorylates E-cadherin. (ox.ac.uk)
Moreover, PKCα directly phosphorylates cortactin in vitro at S135/T145/S172, mitigating both cortactin's actin-binding and actin-crosslinking activity, whereas cellular expression of a phosphorylation-mimetic cortactin mutant hinders filopodial formation with a significant decrease of actin bundles. (elsevierpure.com)
This enzyme phosphorylates tyrosine residues located in the C-terminal end of Src-family kinases (SFKs) including SRC, HCK, FYN, LCK, LYN and YES1. (wikipedia.org)

Adapters bind motor proteins to cargoes and therefore play essential roles in Kinesin-1 mediated intracellular transport. (duke.edu)

S364A - Strong decrease of phosphorylation by PRKACA (the catalytic subunit of protein kinase A). Csk's interaction with a phosphatase ("Lyp", gene product of PTPN22) is possibly associated with the increased autoimmune diseases associated with PTPN22 mutations. (wikipedia.org)
AKIP1-induced hypertrophy was predominantly determined by an increase in cardiomyocyte length, which was associated with reductions in p90 ribosomal S6 kinase 3 (RSK3), increments of phosphatase 2A catalytic subunit (PP2Ac) and dephosphorylation of serum response factor (SRF). (nature.com)

Genetic inactivation either of dopamine D 2 receptors or of adenosine A 2A receptors reduces the phosphorylation of DARPP-32 at Thr34 and the motor depression produced by CP55,940. (jneurosci.org)
Phorbol ester receptors and protein kinase C levels were critically dependent on the culture age. (uky.edu)
Current research focuses on the role of G protein-coupled receptors, in particular GPR75, which we believe could be a novel target and possible circulating biomarker for pulmonary arterial hypertension. (abdn.ac.uk)
Returning to the UK in 1991 as a post doctoral fellow at the University of Leicester with Stefan Nahorski allowed Andrew to research mechanisms of regulation of G protein coupled receptors (GPCRs). (gla.ac.uk)
what two ways do G-protein coupled receptors (metabotropic receptors) work? (flashcardmachine.com)

Tyrosine-protein kinase CSK also known as C-terminal Src kinase is an enzyme that, in humans, is encoded by the CSK gene. (wikipedia.org)
The members of the DNA Repair Interest Group perform research in areas including DNA repair enzymology and fine structure, mutagenesis, gene and cell cycle regulation, protein structure, and human disease. (nih.gov)
Immediate early gene transcription occurs in the first 4 hours following viral infection, when key regulatory proteins that allow the virus to take control of cellular machinery are made. (medscape.com)
Early gene products include DNA replication proteins and some structural proteins. (medscape.com)
The late gene products are made approximately 24 hours after infection, and these proteins are chiefly structural proteins that are involved in virion assembly and egress. (medscape.com)
The MCOLN1 gene provides instructions for making a protein called mucolipin-1. (medlineplus.gov)
For discoveries elucidating how gene expression is influenced by chemical modification of histones-the proteins that package DNA within chromosomes. (nih.gov)
Evidence That the Transforming Gene of Avian Sarcoma Virus Encodes a Protein Kinase Associated with a Phosphoprotein. (nih.gov)
It is a protein_coding_gene from Drosophila melanogaster. (yeastrc.org)

In contrast, point mutation of Thr75, a site on DARPP-32 specifically phosphorylated by cyclin-dependent kinase 5, does not affect the behavioral response to CP55,940. (jneurosci.org)
The cyclin-dependent kinase Cdk2 associates with cyclins A, D, and E and has been implicated in the control of the G1 to S phase transition in mammals. (nih.gov)
Red No. 3, beta-estradiol, and DDT increase ER site-specific DNA binding to the estrogen response element in HTB 133 cells and increase cyclin-dependent kinase 2 activity in MCF-7 breast cancer cells. (researchgate.net)

Moreover, the stimulation of PKC attenuated the phosphorylation of Akt induced by IGF-1 while enhancing that of CREB. (biomedcentral.com)
We utilized a novel multiple-electrode stimulator to produce LTP in a substantial portion of the synapses in a hippocampal CA1 minislice and tested the effects of such stimulation on the presynaptic protein synapsin I. LTP-inducing stimulation produced a long-lasting 6-fold increase in the phosphorylation of synapsin I at its Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) sites without affecting synapsin I levels. (ox.ac.uk)

In contrast, only IL-3 stimulated detectable increases in both myelin basic protein kinase and mitogen-activated protein (MAP) kinase kinase in in vitro assays, although in more specific MAP kinase activity assays a very slight increase in the activity of this enzyme was observed after 6 h at the permissive temperature. (hud.ac.uk)

Characterization of protein kinase C beta isoform's action on retinoblastoma protein phosphorylation, vascular endothelial growth factor-induced endothelial cell proliferation, and retinal neovascularization. (bvsalud.org)
Association of PKC beta 2 isoform with retinoblastoma protein was discovered in retinal endothelial cells , and PKC beta 2 isoform increased retinoblastoma phosphorylation under basal and VEGF -stimulated conditions. (bvsalud.org)

Highly purified Sendai virus contained a protein kinase activity which catalysed the phosphorylation of endogenous polypeptides or exogenous protamine sulphate. (northwestern.edu)
Ca 2+ -phosphatidylserine-sensitive phosphorylation of endogenous substrates was more marked than that observed in the presence of cyclic AMP or Ca 2+ and calmodulin. (uky.edu)

Using a detergent and high salt solubilization procedure, the protein kinase activity was found associated within glycoprotein free virus particles but not with the nucleocapsid associated polypeptides. (northwestern.edu)
Essentially all cellular processes are controlled by reversible protein phosphorylation, and deregulated kinase activity is a hallmark of diseases such as cancer and diabetes. (biotech-calendar.com)
CSK acts by suppressing the activity of the Src family of protein kinases by phosphorylation of Src family members at a conserved C-terminal tail site in Src. (wikipedia.org)
The potential functional consequences of PKC beta-induced retinoblastoma phosphorylation could include enhanced E2 promoter binding factor transcriptional activity and increased VEGF -induced endothelial cell proliferation. (bvsalud.org)
Here, we have shown that the enzymatic activity of topoisomerase II alpha protein purified from HeLa cell nuclei was strongly enhanced following phosphorylation by protein kinase C. We have investigated the possibility that this kinase is involved in cell cycle phase-specific phosphorylation of topoisomerase II alpha in HeLa cells. (ox.ac.uk)
Our results implicate protein kinase C in the cell cycle phase-dependent modulation of topoisomerase II alpha enzymatic activity in human cells. (ox.ac.uk)
Phorbol ester binding sites were associated with protein kinase C, which represented a major protein kinase activity in primary neuronal cultures. (uky.edu)
Our results indicate that LTP expression is accompanied by persistent changes in presynaptic phosphorylation, and specifically that presynaptic CaM kinase II activity and synapsin I phosphorylation may be involved in LTP expression. (ox.ac.uk)
It has been shown previously that ribosomal protein S3 (rpS3) has an endonuclease activity, which is increased by protein kinase Cδ (PKCδ)-dependent phosphorylation. (korea.ac.kr)
A temperature-sensitive mutant of the v-Abl protein has previously been shown to exhibit tyrosine protein kinase activity in Interleukin 3 (IL-3)- dependent IC.DP cells grown at the permissive temperature (32 °C) but not at the restrictive temperature (39 °C). These IC.DP cells are dependent on IL- 3 for suppression of apoptosis at 39 °C, but at 32 °C cells will survive without added growth factor. (hud.ac.uk)
Enables kinase binding activity and protein serine/threonine kinase activator activity. (nih.gov)
High salt increases phosphorylation of NFAT5, which in turn stimulates its localization to the nucleus, its binding to OREs, and its transactivating activity. (nih.gov)
Interestingly, elevated ROS and activity of the DNA damage response protein, ATM, contribute to NaCl-induced activation of NFAT5. (nih.gov)

2. Neurotensin stimulates protein kinase C-dependent mitogenic signaling in human pancreatic carcinoma cell line PANC-1. (nih.gov)

PKD1 is a serine/threonine-specific kinase known to play a role in multiple cellular processes including apoptosis, cytoskeleton remodeling, and invasion. (ox.ac.uk)
ASK3, a novel member of the apoptosis signal-regulating kinase family, is essential for stress-induced cell death in HeLa cells. (nih.gov)
Structure-based mechanism of preferential complex formation by apoptosis signal-regulating kinases. (nih.gov)

This, in turn, prevents dephosphorylation of downstream target proteins, thereby amplifying the effects produced by activation of the cAMP/PKA cascade ( Greengard, 2001 ). (jneurosci.org)
Accordingly, the conference will have an interdisciplinary focus, bringing together experts from diverse fields unified by a common interest in protein phosphorylation/dephosphorylation as a regulatory mechanism. (biotech-calendar.com)
Dephosphorylation of specific proteins ranging between 50-110 kD and the appearance of a 28-kD tyrosine phosphorylated protein were seen within 30 s in human B cell lines. (silverchair.com)
The dephosphorylation was reversed and the 28-kD protein was dephosphorylated in cells stimulated for 1 min. (silverchair.com)
In this study, we examined phosphatases involved in rpS3 dephosphorylation, and we determined that rpS3 is specifically dephosphorylated by protein phosphatase 2A (PP2A). (korea.ac.kr)

By immunoprecipitation assay, rpS3 only interacted with PP2Ac but not with protein phosphatase 1. (korea.ac.kr)

non-olfactory GPCRs, ion channels, and protein kinases. (nih.gov)
Now at the University of Glasgow Andrew has established the Centre for Translational Pharmacology aimed at drawing together his interests in GPCRs and protein kinases into a Centre focused on defining the novel paradigms in pharmacology that will allow for the rational design of next generation drugs. (gla.ac.uk)

Phosphorylation of p70 Ribosomal Protein S6 Kinase β-1 is an Independent Prognostic Parameter in Metastatic Colorectal Cancer. (medscape.com)

The cAMP activates protein kinase A (PKA), which initiates signaling that causes an accumulation of AQP2 in the plasma membrane of the cells facilitating water reabsorption from primary urine and fine-tuning of body water homeostasis. (mdpi.com)

Our data suggest that PP2A protects centromeric cohesion by opposing CK1delta/epsilon- and DDK-dependent phosphorylation of Rec8. (nih.gov)

Point mutation of Thr34, the protein kinase A (PKA) phosphorylation site of DARPP-32, produces a similar reduction in the effect of the CB1 agonist. (jneurosci.org)
We use protein design to engineer a functionally silent yet structurally significant mutation into the active site of the protein of interest. (berkeley.edu)

We also suggest the involvement of myristoylated alanine-rich C kinase substrate (MARCKS), which is known as a downstream target of PKC-ε, in ADP of LDCV exocytosis. (jneurosci.org)

To identify potential Cdk2 regulators, we have employed an improved two-hybrid system to isolate human genes encoding Cdk-interacting proteins (Cips). (nih.gov)

CIP1 encodes a novel 21 kd protein that is found in cyclin A, cyclin D1, cyclin E, and Cdk2 immunoprecipitates. (nih.gov)

The main advantage of our chemical approach to these problems is that the small organic molecules we make to inhibit single kinases in whole animals are much better models of how potential drugs which target the same proteins might actually work, hopefully saving time in avoiding failed clinical trials. (berkeley.edu)
The key problem with using small-molecules to control processes inside cells is that most often these reagents inhibit closely related enzymes with equal potency, making dissection of each individual protein impossible. (berkeley.edu)
Protein kinases may activate or inhibit ion channels by phosphorylation. (flashcardmachine.com)

These findings define a mechanism by which survival signals inactivate a proapoptotic Bcl-2 family member, and suggest a role for 14-3-3 proteins as cofactors that regulate sequential protein phosphorylation events. (harvard.edu)

By analysis of the adaptor proteins NCK1 and GRB2 mutants we further show that the negative loop on p38 is mediated by c-ABL phosphorylation at tyrosine 105 of the adaptor protein NCK1, while the phosphorylation at tyrosine 209 of GRB2 down-modulates ERK1/2 and JNKs signaling. (unisi.it)

Dr. Burg's lab has identified the amino acids in NFAT5 that become phosphorylated, as well as protein kinases, phosphatases, and other proteins that are involved. (nih.gov)

Ser-155 phosphorylation disrupts the binding of BAD to prosurvival Bcl-2 proteins and thereby promotes cell survival. (harvard.edu)

IGF-1 plays anti-apoptotic and other functions by activating multiple signaling pathways including Akt kinase, a serine/threonine kinase essential for cell survival. (biomedcentral.com)

Ca2+/calmodulin-dependent protein kinase II phosphorylation of the presynaptic protein synapsin I is persistently increased during long-term potentiation. (ox.ac.uk)

PKC activation by phorbol 12-myristate 13-acetate causes cortactin phosphorylation, filopodial retraction and F-actin-bundle loss. (elsevierpure.com)
Actin filament disassembly induced by MARCKS phosphorylation was also significantly blocked by transfection of DN-PKC-ε. (jneurosci.org)

Activation of this transcription factor requires the phosphorylation of the Ser-133 residue which increases its association with CREB-binding protein [ 32 ]. (biomedcentral.com)

This makes choosing a residue which is conserved in the entire protein family critical for the success of the method. (berkeley.edu)

Here, we show that cargoes transported by the Kinesin-1 adapter FEZ1 are enriched for presynaptic components and identify that specific phosphorylation of FEZ1 at its serine 58 regulatory site is mediated by microtubule affinity-regulating kinases (MARK/PAR-1). (duke.edu)

Activation of protein kinase C (PKC) enhances the angiogenic process and is involved in the signaling of vascular endothelial growth factor ( VEGF ). (bvsalud.org)
Mechanistically, AKIP1 promoted exercise-induced activation of protein kinase B (Akt), downregulation of CCAAT Enhancer Binding Protein Beta (C/EBPβ) and de-repression of Cbp/p300 interacting transactivator with Glu/Asp rich carboxy-terminal domain 4 (CITED4). (nature.com)

Finally, we show that TTBK2 is also required for efficient phosphorylation of many S/T sites in CEP164 and provide evidence that TTBK2-induced phosphorylations of CEP164 modulate its function, which in turn seems relevant for the process of cilia formation. (muni.cz)
To identify sites of phosphorylation, and the kinase(s) responsible for this modification, oligohistidine-tagged recombinant domains of topoisomerase II alpha protein were overexpressed in Escherichia coli and purified by affinity chromatography. (ox.ac.uk)
Thus, about a 20-fold increase in binding sites occurred during the first week in culture and was accompanied by a corresponding increase in Ca 2+ -phosphatidylserine-sensitive protein phosphorylation in solution neuronal extracts. (uky.edu)
Moreover, the PP2Ac association with rpS3 was identified in cells transfected with wild-type rpS3 but not with mutant rpS3 lacking PKCδ phosphorylation sites. (korea.ac.kr)
Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein. (yeastgenome.org)

Anatomy21

Organisms9

Chemicals and Drugs162

Analytical, Diagnostic and Therapeutic Techniques and Equipment16

Phenomena and Processes43

Disciplines and Occupations1

Information Science3

Pathway12

Inhibitors5

Extracellular2

MAPK4

Induces2

Inhibitor7

Phosphatidylinositol 3-kina2

Signal Transduction1

Tyrosine phosphorylation7

Regulates4

Suggests that phosphorylation2

Casein kinase1

Receptor13

Neurons4

Phosphorylates3

Intracellular1

Subunit2

Receptors5

Gene9

Cyclin-depend3

Stimulation2

Assays1

Retinoblastoma protein2

Endogenous2

Activity13

Stimulates1

Apoptosis3

Dephosphorylation5

Immunoprecipitation1

GPCRs2

Ribosomal1

Activates protein1

Epsilon1

Mutation2

Downstream1

Genes1

Encodes1

Inhibit3

Sequential1

JNKs1

Amino1

Disrupts1

Pathways1

Presynaptic protein1

Actin2

Increases1

Residue1

Microtubule1

Activation of protein2

Sites5