Loading...
  • Biol
  • I have started measuring in-situ Protein Kinase C activity in permeabilized cells grown in 96-well plates, based on a few references from the literature such as: Heasley L.E., J.Biol.Chem. (bio.net)
  • residues
  • In the case of classical MAP kinases, the activation loop contains a characteristic TxY (threonine-x-tyrosine) motif (TEY in mammalian ERK1 and ERK2 , TDY in ERK5 , TPY in JNKs , TGY in p38 kinases ) that needs to be phosphorylated on both the threonine and the tyrosine residues in order to lock the kinase domain in a catalytically competent conformation. (wikipedia.org)
  • The catalytic subunit contains the active site, a series of canonical residues found in protein kinases that bind and hydrolyse ATP and a domain to bind the regulatory subunit. (wikipedia.org)
  • mammalian
  • Thus the role of mammalian ERK1/2 kinases as regulators of cell proliferation is not a generic, but a highly specialized function. (wikipedia.org)
  • The identification of protein kinase C iota as a regulator of the Mammalian heat shock response using functional genomic screens. (ebi.ac.uk)
  • Studies on a cyclic nucleotide-independent protein kinase and its proenzyme in mammalian tissues. (ebi.ac.uk)
  • Literature
  • 1 demonstrated that the potent tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) could stimulate protein kinase C activity, protein kinase C caught the attention of many scientists and has resulted in a vast and ever-growing literature on the role of protein kinase C in numerous biologic processes induced by TPA. (springer.com)
  • p>An evidence describes the source of an annotation, e.g. an experiment that has been published in the scientific literature, an orthologous protein, a record from another database, etc. (uniprot.org)
  • gene
  • Norway rat protein-coding gene Mapk1. (nih.gov)
  • par-1, a gene required for establishing polarity in C. elegans embryos, encodes a putative Ser/Thr kinase that is asymmetrically distributed. (ebi.ac.uk)
  • amino
  • The Structural Classification of Proteins (SCOP) database is a largely manual classification of protein structural domains based on similarities of their amino acid sequences and three-dimensional structures. (ebi.ac.uk)
  • novel
  • Broad in its range of techniques and thoroughly detailed to help ensure experimental success, Protein Kinase Protocols offers both novice and experienced investigators powerful tools for understanding the functional roles of specific protein kinases within signaling cascades and for identification and evaluation of novel therapeutic targets. (springer.com)
  • Cloning of a novel phosphatidylinositol kinase-related kinase: characterization of the human SMG-1 RNA surveillance protein. (springer.com)
  • Protein kinase C, epsilon is a novel type of protein kinase C (nPKC). (ebi.ac.uk)
  • nonsense-media
  • The nonsense-mediated mRNA decay SMG-1 kinase is regulated by large-scale conformational changes controlled by SMG-8. (springer.com)
  • Grimson A, O'Connor S, Newman CL, Anderson P. SMG-1 is a phosphatidylinositol kinase-related protein kinase required for nonsense-mediated mRNA Decay in Caenorhabditis elegans . (springer.com)
  • SMG-1 is a phosphatidylinositol kinase-related protein kinase required for nonsense-mediated mRNA Decay in Caenorhabditis elegans. (ebi.ac.uk)
  • Inhibitor
  • Ponatinib is indicated for the treatment of adult patients with chronic phase, accelerated phase, or blast phase chronic myeloid leukemia (CML) that is resistant or intolerant to prior tyrosine kinase inhibitor therapy or Philadelphia chromosome positive acute lymphoblastic leukemia (Ph+ALL) that is resistant or intolerant to prior tyrosine kinase inhibitor therapy. (drugbank.ca)
  • Tafinlar is a kinase inhibitor that was initially indicated as a single agent for the treatment of patients with unresectable or metastatic melanoma with BRAF V600E mutation as detected by an FDA-approved test [FDA label]. (drugbank.ca)
  • Ceritinib is a kinase inhibitor indicated for the treatment of patients with anaplastic lymphoma kinase (ALK)-positive metastatic non-small cell lung cancer (NSCLC) who have progressed on or are intolerant to crizotinib. (drugbank.ca)
  • subunits
  • PKA is also commonly known as cAMP-dependent protein kinase, because it has traditionally been thought to be activated through release of the catalytic subunits when levels of the second messenger cAMP rise in response to a variety of signals. (wikipedia.org)
  • phosphorylates
  • These were formerly included in EC number "2.7.1.37", which was a general EC number for any enzyme that phosphorylates proteins while converting ATP to ADP (i.e. (wikipedia.org)
  • genes
  • HuSH-29 Kinase Collection contains 512 genes cloned in pRFP-C-RS vector allowing researchers to monitor transfection. (origene.com)
  • activity
  • Phosphorylation usually results in a functional change of the target protein by changing enzyme activity, cellular location, or association with other proteins. (ebi.ac.uk)
  • Shani G, Henis-Korenblit S, Jona G, Gileadi O, Eisenstein M, Ziv T, Admon A, Kimchi A. Autophosphorylation restrains the apoptotic activity of DRP-1 kinase by controlling dimerization and calmodulin binding. (ebi.ac.uk)
  • Doublecortin microtubule affinity is regulated by a balance of kinase and phosphatase activity at the leading edge of migrating neurons. (ebi.ac.uk)
  • polarity
  • The polarity protein partitioning-defective 1 (PAR-1) regulates dendritic spine morphogenesis through phosphorylating postsynaptic density protein 95 (PSD-95). (ebi.ac.uk)
  • Loss of the Par-1b/MARK2 polarity kinase leads to increased metabolic rate, decreased adiposity, and insulin hypersensitivity in vivo. (ebi.ac.uk)