Protein interactions rna small nuclear. Medical search. Frequent questions

Cumulative evidence shows that only ∼ 2 percent of protein-coding genes are in the human genome and the remaining ∼ 98 percent of the human genome are classified as non-coding RNAs (ncRNAs) [ 1 ]. (biomedcentral.com)
Many of these signatures are found in NP, PA, and PB2 genes (viral ribonucleoproteins [RNPs]) and are mostly located in the functional domains related to RNP-RNP interactions that are important for viral replication. (cdc.gov)
Although many research efforts have focused on the molecular evolution of specific genes of influenza viruses, comprehensive comparisons among the nucleotide sequences of all 8 genomic segments and among the 11 encoded protein sequences have not been extensively reported. (cdc.gov)
The majority of human genes that encode proteins undergo alternative pre-mRNA splicing and mutations that affect splicing are more prevalent than previously thought. (ox.ac.uk)
Use of the microarray analysis is often restricted by the known gene sequences arrayed on the chip, whereas RNA sequencing is not dependent on pre-existing databases of expressed genes and, therefore, provides an unbiased and more complete view of gene expression profiles [ 17 ], including lncRNA. (biomedcentral.com)
In humans, the CRYAA gene encodes a 173 amino acid residue protein by single copy genes located on chromosome 21. (molvis.org)
Whithout mutations we would know very little about inheritance and the existence of genes. (intechopen.com)
To identify genes, signaling pathways and networks that are controlled by SRC-3 and potentially important for hormone-dependent decidualization, we performed RNA-sequencing on HESCs in which SRC-3 levels were significantly reduced at the time of administering the deciduogenic stimulus. (frontiersin.org)
The three genes encoding these proteins were inserted individually into vaccinia virus GLV-1h68 genome under the control of an identical promoter. (uni-wuerzburg.de)
These products are often proteins, but in non-protein coding genes such as ribosomal RNA (rRNA), transfer RNA (tRNA), or small nuclear RNA (snRNA) genes, the product is a functional RNA. (nutritionaloutlook.com)
While the selective inactivation of Ophiostoma genes by insertional mutagenesis remains a challenge, an alternative approach based on RNA interference is now available for down-regulating the expression of targeted genes. (sisef.it)
Numerous genes transcribed in oocytes are involved in multiple aspects of cell maintenance and protection, including metabolism, signal perception and transduction, RNA processing, cell cycle, defense against pathogens and DNA damage. (biomedcentral.com)
Below is a non-exhaustive list of in-house infrastructures that are categorized into three overarching themes: bio-imaging, proteins, genes & cells and other resources. (lu.se)
In addition to infrastructures for bioimaging, protein and genes & cells, we also provide other resources e.g., databases, networks and specialized labs. (lu.se)
In some cases, mutations in nuclear DNA are responsible for the condition, including mutations in the POLG , TWNK , RRM2B , and SLC25A4 genes, among others. (medlineplus.gov)
These mutations occur in genes that provide instructions for making molecules called transfer RNAs. (medlineplus.gov)
We use these engineered Cas9 activation complexes to investigate sgRNA targeting rules for effective transcriptional activation, demonstrate multiplexed activation of 10 genes simultaneously, and upregulate long intergenic non-coding RNA (lincRNA) transcripts. (cdc.gov)

RNP-based processive anti-termination underlies the switching from immediate-early to delayed-early gene expression in other lambdoid phages as well as ribosomal RNA synthesis in Escherichia coli . (fu-berlin.de)
Huang YH, Said N, Loll B, Wahl MC (2019) Structural basis for the function of SuhB as a transcription factor in ribosomal RNA synthesis. (fu-berlin.de)
English Ribosomal proteins are synthesized in the cytoplasm, before nuclear import and assembly with ribosomal RNA (rRNA). (unifr.ch)
Specific tertiary structure types are observed for transfer RNA (tRNA), ribosomal RNA (rRNA), small nuclear RNA (snRNA), certain introns, and ribozymes. (biosyn.com)

This process is carried out by the human spliceosome machinery, in which over 300 proteins sequentially assemble with uridine-rich small nuclear RNA molecules (U snRNAs) to form distinct small nuclear ribonucleoprotein complexes (snRNPs). (biomedcentral.com)
MBL Grenoble's researchers focus mainly on RNA biology and infection biology, in particular on the structural molecular biology of protein-RNA complexes involved in cellular gene expression and host-pathogen interactions. (embl.org)
Moreover, RNA-protein complexes (RNPs) provide glimpses at the molecular ancestry of modern cells, which most likely evolved from an RNA-dominated world. (fu-berlin.de)
then, a specific antibody immunoprecipitates protein-associated RNA-DNA complexes. (epigenie.com)
Indeed, the identification of Kcnq1ot1, which targets Polycomb complexes to repressed genomic domains, and the significant overlap of ncRNAs isolated via a conventional technique suggest that RedChIP represents the way forward for studies of RNA-protein-DNA interactions in live cells. (epigenie.com)
Yeast two-hybrid screenings using SMYB1 as bait identified a partner in S. mansoni similar to the SmD3 protein of Drosophila melanogaster (SmRNP), which is important in the assembly of small nuclear ribonucleoprotein complexes. (cambridge.org)
Over the last decades, a variety of RNA methods have been developed for the study of RNA-DNA, RNA-RNA, and RNA-protein interactions including RNA complexes with ligand molecules. (biosyn.com)

Similar to other homologues, SMYB1 is able to bind double- and single-stranded DNA, as well as RNA molecules. (cambridge.org)
RNA molecules are functionally diverse and involved in many cellular processes such as catalysis, ligand binding, and protein recognition. (biosyn.com)
RNA molecules are structural flexible and can adopt different structures. (biosyn.com)
RNAs are single-stranded molecules and the unlinked monomer building blocks are known as nucleotides. (biosyn.com)
RNA molecules are generally folded into compact and defined tertiary structures. (biosyn.com)
RNA molecules in solution may adopt secondary structures that are only partially determined by thermodynamics since RNA molecules can undergo conformational changes during interaction with other RNAs, RNA binding proteins or RNA binding peptides. (biosyn.com)

U7 snRNA-associated Sm-like protein LSm10 is a protein that in humans is encoded by the LSM10 gene. (wikipedia.org)
We propose a model for U6 snRNP assembly that explains how evolutionarily divergent and seemingly antagonistic proteins cooperate to protect and chaperone the nascent snRNA during its journey to the spliceosome.The mechanism of U6 small nuclear ribonucleoprotein (snRNP) biogenesis is not well understood. (cornell.edu)

Sequential assembly of the human spliceosome on RNA transcripts regulates splicing across the human transcriptome. (biomedcentral.com)
Recent studies have suggested that eukaryotic genomes encode a large number of functional transcripts of non-coding RNAs (ncRNAs), including housekeeping and regulatory RNAs [ 1 - 3 ]. (biomedcentral.com)
PSI directly regulates the AS of at least one-third of these transcripts, suggesting that PSI-U1 snRNP interactions coordinate the behavioral network underlying courtship behavior. (princeton.edu)
Quantitative estimation of RNA transcripts suggests persistence of Pax-6 expression in the postembryonic chick retina. (uclahealth.org)
Transcripts found in unfertilized oocytes also encoded a large number of proteins implicated in cell adherence, tight junction and focal adhesion, suggesting high complexity in terms of structure and cellular interactions in embryos prior to midblastula transition (MBT). (biomedcentral.com)
A hallmark of events observed during segmentation was the induction of multiple transcription factors, including a large group of homeobox proteins in pace with decay of a large fraction of maternal transcripts. (biomedcentral.com)

Competition for PRPF8 availability alters the transcription-coupled splicing of RNAs in which weak 5′ splice sites predominate, enabling diversification of human gene expression during biological processes like mitosis. (biomedcentral.com)
Numerous fundamental cellular processes, including virtually every aspect of gene expression and regulation, depend on the cooperation of RNAs and proteins. (fu-berlin.de)
Specific RNAs and proteins associate to build up some of the most complex macromolecular machineries of living cells, such as ribosomes and spliceosomes, which mediate particular steps of gene expression. (fu-berlin.de)
Sequencing of tags created by restriction digestion of cDNA generates gene expression profiles with an absolute count (from one to a few million) of the RNA in the sample. (elimbio.com)
Also, viral infection has proved to have a great impact on cellular small RNA expression and gene expression [ 9 - 11 ]. (hindawi.com)
Small RNAs include microRNA (miRNA), siRNA, tRFs, piRNA, and rasiRNAs, which regulate gene expression in a wide range of processes such as viral replication and host immune response. (hindawi.com)
The characterization of proteins involved in the regulation of gene expression in S. mansoni is of great importance for the understanding of molecular events that control morphological and physiological changes in this parasite. (cambridge.org)
Therapeutic tiny or short modified antisense oligonucleotides (Tiny ASOs) can block gene expression or modulate splice-switching! (biosyn.com)
The characterization of RNA and RNA interactions is closely related to transcription, for example, gene expression levels are investigated within a biological context. (biosyn.com)
Since RNA-binding proteins (RBPs) are key players in the post-transcriptional regulation of gene expression precise knowledge of their binding sites is critical for determining their molecular function and for understanding their roles in cell development and disease. (biosyn.com)
Programmable DNA binding proteins have emerged as an exciting platform for engineering synthetic transcription factors for modulating endogenous gene expression 5 - 11 . (cdc.gov)

RNAs and proteins can also engage in more transient interactions, for example during co-transcriptional regulation of RNA polymerases or during post-transcriptional regulation of (pre-)mRNA life cycles. (fu-berlin.de)
Bacteria transcribe their genomes with the help of multi-subunit RNA polymerases (RNAPs), which comprise two large β and β' subunits that form the active site, two regulatory α subunits and an ω subunit that supports RNAP assembly. (fu-berlin.de)
Full phase separation is prevented because RNA remains tethered to transcribed euchromatin through RNA polymerases. (nature.com)
RNA polymerases read the codes from specific areas of the DNA and transcribe the information into a mRNA copy of the DNA. (cdc.gov)

The approach was tested on polypyrimidine tract binding protein 1 and U1 small nuclear RNP. (nih.gov)

Recent highlights include: the structure and function of long non-coding RNAs , the structure of the integrator complex , the determination of the mechanism of action of secreted Legionella effector proteins , and the structural and mechanistic analysis of the transcription/replication machines of influenza virus and lassa virus . (embl.org)
During Adv DNA replication, host cellular proteins such as nuclear factor I and POU2F1 are used by Adv [ 13 , 14 ]. (hindawi.com)
Cryo-electron tomography reveals novel features of a viral RNA replication compartment. (janelia.org)
We used cryo-electron tomography to reveal many previously unrecognized features of Flock house nodavirus (FHV) RNA replication compartments. (janelia.org)
These spherular invaginations of outer mitochondrial membranes are packed with electron-dense RNA fibrils and their volumes are closely correlated with RNA replication template length. (janelia.org)
Each spherule's necked aperture is crowned by a striking cupped ring structure containing multifunctional FHV RNA replication protein A. Subtomogram averaging of these crowns revealed twelve-fold symmetry, concentric flanking protrusions, and a central electron density. (janelia.org)
These results provide new mechanistic insights into positive-strand RNA virus replication compartment structure, assembly, function and control. (janelia.org)
Replication of hepatitis C virus RNA on autophagosomal membranes J Biol Chem. (usc.edu)
Hepatitis C virus translation preferentially depends on active RNA replication PLoS One. (usc.edu)
KIN17, which is known as a DNA and RNA binding protein, is highly expressed in numerous types of human cancers and was discovered to participate in several vital cell behaviors, including DNA replication, damage repair, regulation of cell cycle and RNA processing. (spandidos-publications.com)
Previous studies have indicated that KIN17 is involved in global genome repair, DNA replication, transcription and regulation of the cell cycle as part of a multi-protein complex. (spandidos-publications.com)
Alternatively, following viral DNA release into a cell, cell-virus interaction leads to a dormant (so-called latent) state for a virus where its genome remains in the cell without replication which can later reactive and result in cell lysis. (lu.se)

We investigate the molecular mechanisms, by which RNAs and proteins cooperate to bring about the biological functions of selected RNPs involved in transcription and pre-mRNA splicing. (fu-berlin.de)
By identifying many sGAG-specific interacting proteins, our data provide a resource for upcoming studies aimed at molecular mechanisms and understanding of sGAG cellular effects. (degruyter.com)
The mass sequence data generated in this study provide a cue for future functional and molecular research on wheat-fungus interactions. (biomedcentral.com)
The combination of different biochemical methods with computational modelling allows scientists to gain insight into molecular processes in which RNA is involved. (biosyn.com)
RNA is a long, polymer of ribonucleoside monophosphate molecular units or building blocks joined together by phosphodiester linkages. (biosyn.com)
During the last decades, the development of ever more powerful genetic, molecular and omic approaches has provided plant pathologists with a wide array of experimental tools for elucidating the intricacies of plant-pathogen interactions and proposing new control strategies. (sisef.it)
Two interrelated paradigms currently under investigation are: 1) Molecular basis of the differential expression of the gene for the small heat shock protein Alpha B-crystallin, a protein associated with with vision (e.g. in the ocular lens), oncogenesis (e.g. in breast cancer) and neural degeneration (e.g. in Alzheimer's disease). (uclahealth.org)
Our facilities provide the opportunity to study protein structure, molecular probes and drug design, system biology and molecular interactions in cells and tissues. (lu.se)
With the help of various forms of mass spectrometry, synchrotron radiation, protein production & labelling, and bioinformatics, our facilities provide the opportunity to study protein structure and dynamics, molecular probes and drug design. (lu.se)

USP15 Deubiquitinates TUT1 Associated with RNA Metabolism and Maintains Cerebellar Homeostasis. (nih.gov)
TDP-43 protein plays an important role in regulating transcriptional repression, RNA metabolism and splicing. (nature.com)
Here we demonstrate that SMYB1 is located in the cytoplasm of cells from different life-cycle stages of S. mansoni , suggesting that this protein is probably acting in mRNA metabolism in the cytoplasm and corroborating previous findings from our group that showed its ability to bind RNA. (cambridge.org)

The heterogeneous nuclear ribonucleoprotein (hnRNP) C proteins, among the most abundant pre-mRNA-binding proteins in the eukaryotic nucleus, have a single RNP motif RNA-binding domain. (korea.ac.kr)
Many eukaryotic precursor messenger RNAs (pre-mRNAs) bear coding regions (exons) interspersed with non-coding intervening sequences (introns). (fu-berlin.de)

instead, they combined RNA-DNA proximity ligation ( Red-C ) with chromatin immunoprecipitation (ChIP) to create RedChIP, which identifies RNAs associated with a DNA-bound protein of interest. (epigenie.com)
In eukaryotes, DNA is packed inside the cell nucleus in the form of chromatin, which consists of DNA, proteins such as histones, and RNA. (nature.com)
We show that accumulation of RNA induces formation of transcription pockets which displace transcriptionally inactive chromatin. (nature.com)
It has been suggested, for example, that nuclear bodies, including those formed from RNA and RNA-binding proteins (RBPs), can displace transcriptionally inactive chromatin 18 . (nature.com)
As the active nuclear compartment contains high levels of RNA and RNA-binding proteins 1 , 5 , this provides a potential mechanism for the selective exclusion of inactive chromatin from the active compartment. (nature.com)
These characteristics correlate with a distinct nuclear architecture, epigenetic signatures enriched for active chromatin marks and hyperdynamic binding of structural chromatin proteins. (biomedcentral.com)
Recently, several chromatin-related proteins have been shown to regulate ESC pluripotency and/or differentiation, yet the role of the major heterochromatin proteins in pluripotency is unknown. (biomedcentral.com)

We subsequently validated the robustness of those signatures with human and avian protein sequences downloaded from Influenza Virus Resources at the National Center for Biotechnology Information (NCBI) ( http://www.ncbi.nlm.nih.gov/genomes/FLU/FLU.html ). (cdc.gov)
These viruses replicate their RNA genomes in novel, membrane-bounded mini-organelles, but the organization of viral proteins and RNAs in these compartments is largely unknown. (janelia.org)
The small genomes of the DED fungi also facilitate genomic analyses. (sisef.it)

RNA synthesis is terminated intrinsically, when the elongation complex transcribes a stable RNA hairpin followed by a uridine-rich stretch, or with the aid of transcription termination factor ρ. (fu-berlin.de)

According to its size, regulatory ncRNAs can be further classified as small ncRNA ( ∼ 18-31nt, such as miRNA, siRNA, and piRNA), medium ncRNA ( ∼ 31-200nt) and long ncRNA (from 200nt up to several hundred kb, such as lincRNA and microRNA) [ 4 ]. (biomedcentral.com)
While RNA-DNA proximity ligation techniques map genome-wide ncRNA interactions , they don´t describe the proteins involved. (epigenie.com)
Non-coding RNA (ncRNA) such as microRNA (miRNA) and long non-coding RNA (lncRNA) regulates the translation and/or stability of multiple mRNA targets either directly or by modulation of signal transduction pathways. (frontiersin.org)
miR-301a is initially identified as a novel ncRNA to be upregulated in T-helper cells in response to myelin oligodendrocyte protein antigen in the multiple sclerosis ( 8 ). (frontiersin.org)

Description: RNA profiling data sets generated by the Mouse ENCODE project. (nih.gov)

Now, "RedChIP" has us stampeding in anticipation towards the exploration of the complex interactions taking place between RNA, DNA, and proteins in the nucleus. (epigenie.com)
Transgenic retina-specific expression of AD5 reveals that stable AD5 protein is initially localized in the nucleus but later found in the cytoplasm concurrent with progressing rod outer segment degeneration and apoptosis. (uni-wuerzburg.de)
Although most DNA is packaged in chromosomes within the nucleus (nuclear DNA), mitochondria also have a small amount of their own DNA, called mitochondrial DNA or mtDNA. (medlineplus.gov)
Artificially colored electron micrographs of HSV-1 at the cell membrane (a), in transport to the nucleus (b), and bound at a nuclear pore complex (NPC) embedded within the nuclear envelope (c). (lu.se)

Here, we identify a transport adaptor, symportin 1 (Syo1), that facilitates synchronized coimport of the two 5S-rRNA binding proteins Rpl5 and Rpl11. (unifr.ch)

The mechanism of pre-mRNA splicing is highly complex, requiring multiple interactions between pre-mRNA, small nuclear ribonucleoproteins and splicing factor proteins. (ox.ac.uk)

A large number of experimental studies show that the mutation and regulation of long non-coding RNAs (lncRNAs) are associated with various human diseases. (biomedcentral.com)
Long non-coding RNAs (lncRNAs) play an increasingly important role in some fundamental biological processes such as translational regulation, cell cycle regulation, epigenetic regulation, splicing, differentiation, and immune response [ 5 ]. (biomedcentral.com)
Regulation of this process is even more complicated, relying on loosely defined cis-acting regulatory sequence elements, trans-acting protein factors and cellular responses to varying environmental conditions. (ox.ac.uk)
Many different human diseases can be caused by errors in RNA splicing or its regulation. (ox.ac.uk)
Although the current generation of dCas9-based transcription activators is able to achieve up-regulation of some endogenous loci, the magnitude of transcriptional up-regulation achieved by individual single-guide RNAs (sgRNAs) 12 typically ranges from low to ineffective 8 - 11 . (cdc.gov)

The α 2 ββ'ω core enzyme cooperates with transcription factors and responds to signals on DNA templates and nascent RNAs to achieve full functionality in vivo . (fu-berlin.de)

Orthologous to human LSM7 (LSM7 homolog, U6 small nuclear RNA and mRNA degradation associated). (nih.gov)
Furthermore, pumilio homolog 2 (PUM2), a RNA-binding protein, mediated the packaging of miRNA-130a into exosomes. (dovepress.com)

Bioinformatic algorithms allowing predicting of biomolecular folding for proteins, peptides, and RNAs, even though sometimes successful, have all their limitations. (biosyn.com)
Since there are way too many nutrient/gene interactions to discuss, I'll limit my examples to collagen peptides and resveratrol. (nutritionaloutlook.com)
Collagen peptides refer to a small collagen protein chain. (nutritionaloutlook.com)
The significance of this is that smaller protein chains-or peptides-tend to be easily absorbed and distributed in the body. (nutritionaloutlook.com)
Laboratory research also shows that collagen peptides can stimulate the production of collagen and can be metabolized into even smaller peptides known as dipeptides. (nutritionaloutlook.com)

Here, we show that alterations in RNA splicing patterns across the human transcriptome that occur in conditions of restricted cellular PRPF8 abundance are defined by the altered splicing of introns with weak 5′ splice sites. (biomedcentral.com)
Intrinsic antiviral resistance is a branch of antiviral defence that involves constitutively expressed cellular proteins that act within individual infected cells. (mdpi.com)
In recent years it has been discovered that components of cellular nuclear structures known as ND10 or PML nuclear bodies contribute to intrinsic resistance against a variety of viruses, notably of the herpesvirus family. (mdpi.com)
Specialized cell structures called ribosomes are the cellular organelles that actually synthesize the proteins (RNA transcription). (cdc.gov)

Many biological roles have been assigned to PrP, leading to presume that this protein can act in multicellular process, interacting with many biological macromolecules. (unina.it)
The nucleic acids (NA) correlation with prion protein has ever been an issue of debate since the "protein only" hypothesis brought a new biological paradigm. (unina.it)
Protein-protein interactions are important events in all biological processes, since most proteins execute their functions through large supramolecular structures. (cambridge.org)
Here we assessed three candidate proteins with the potential to be used as biomarkers in biological fluids: two glucuronidases from E. coli (GusA) and Staphylococcus sp. (uni-wuerzburg.de)
The laboratory is interested in (a) elucidating developmental and tissue-specific control of the heat shock promoter of the Alpha B-crystallin gene and (b) understanding the biological function of the Alpha B-crystallin protein. (uclahealth.org)
Furthermore, biological and physical simplicity relative to other biological systems have made viruses an attractive physical model system to study fundamental prosperities of DNA compaction and translocation as well as protein self-assembly using viral capsids. (lu.se)

Small DNA and RNA aptamers have shown to interact with PrP with high affinity. (unina.it)
Here we used an affinity-purification mass spectrometry-based (AP-MS) approach to identify novel and particularly intracellular sGAG-interacting proteins in human bone marrow stromal cells (hBMSC). (degruyter.com)
The mapping of RNA-protein or RNA-RNA interactions by protein pull-down or affinity pull-down methods allow studying RNA structures, as well as RNA-protein, and RNA-RNA interactions. (biosyn.com)
Usb1 processing of U6 RNA dramatically alters its affinity for cognate RNA-binding proteins. (cornell.edu)

Scientists at EMBL Grenoble determine 3D structures of human and viral proteins to understand how they interact with the nucleic acids DNA and RNA. (embl.org)

In other cases, the condition is caused by a single large deletion of mtDNA that is not associated with a mutation in a nuclear DNA gene. (medlineplus.gov)

We reconstitute the post-transcriptional assembly of yeast U6 snRNP in vitro, which occurs through a complex series of handoffs involving 10 proteins (Lhp1, Prp24, Usb1 and Lsm2-8) and anti-cooperative interactions between Prp24 and Lhp1. (cornell.edu)
Here the authors characterize the enzymatic activities and structures of yeast and human U6 RNA processing enzyme Usb1, reconstitute post-transcriptional assembly of yeast U6 snRNP in vitro, and propose a model for U6 snRNP assembly. (cornell.edu)

Sequencing of random-primed cDNA libraries from RNA fractions (nuclear, cytoplasmic, polyA, capped, or small RNA) provides a high-resolution map of all RNA species. (elimbio.com)
Many crowns were associated with long cytoplasmic fibrils, likely to be exported progeny RNA. (janelia.org)
Following TDP-43 sequestration in insoluble aggregates, we observed higher nuclear levels of EIF4A3 and POLDIP3β, whereas nuclear levels of DNMT3A, HNRNPA3, PABPC1 and POLDIP3α dropped and cytoplasmic levels of RANBP1 dropped. (nature.com)
In addition, immunofluorescence signal intensity quantifications showed increased nuclear expression of HNRNPL and YARS and downregulation of cytoplasmic DPCD. (nature.com)
Furthermore, cytoplasmic levels of predominantly nuclear protein ALYREF increased. (nature.com)

We subsequently used 15,785 protein sequences from the National Center for Biotechnology Information (NCBI) to assess the robustness of these signatures and obtained 52 "species-associated" positions. (cdc.gov)

In LDNFSGB, we first construct a comprehensive feature vector to effectively extract the global and local information of lncRNAs and diseases through considering the disease semantic similarity (DISSS), the lncRNA function similarity (LNCFS), the lncRNA Gaussian interaction profile kernel similarity (LNCGS), the disease Gaussian interaction profile kernel similarity (DISGS), and the lncRNA-disease interaction (LNCDIS). (biomedcentral.com)

One-hundred and twenty-seven of 17,225 human full-length proteins were identified that interact with CRYAA. (molvis.org)

Using both X-ray crystallography and nuclear magnetic resonance spectroscopy, UCLA biochemists have examined the structure of p65 protein and its interaction with telomerase RNA. (scitechdaily.com)
Among those are X-ray crystallography, small-angle X-ray scattering, state-of-the art ESI mass spectrometry, microcalorimetry (ITC, DSC), surface plasmon resonance (Biacore 3000), ellipsometry, chromatography, CD spectroscopy and dynamic light scattering (DLS). (lu.se)

In these processes, transcript-borne regulatory RNAs alone or in conjunction with protein factors assemble on the surface of RNAP and accompany the enzyme during further transcription by an RNA looping mechanism, rendering the elongation complex resistant to pause and/or termination signals downstream of the original modification site. (fu-berlin.de)
Transfer RNAs help assemble protein building blocks (amino acids) into functioning proteins. (medlineplus.gov)
The transfer RNAs associated with progressive external ophthalmoplegia are present in mitochondria and help assemble the proteins that carry out the steps of oxidative phosphorylation. (medlineplus.gov)

Functional annotation clustering showed that they belong to cell cycle, organelle or nuclear lumen, protein transport, and DNA binding and repair clusters. (molvis.org)
In an attempt to facilitate the discovery of antiviral agents that target viral polymerase, isolated functional domains such as the PA endonuclease domain, the PB2 cap-binding domain, and the PA-PB1 interaction domains were expressed as screening targets. (hku.hk)

The experimental approach we described should be generally applicable for the screening and identification of amino acids that play a role in the binding of proteins to nucleic acid substrates. (korea.ac.kr)

Respecting the preferentially intracellular localization of sGAG in vesicle-like structures, also the interaction data indicate sGAG-specific modulation of vesicle-based transport processes. (degruyter.com)
Here, we report structures of the U6 RNA processing enzyme Usb1 from yeast and a substrate analog bound complex from humans. (cornell.edu)

The large ~280-kDa U5 snRNP protein PRPF8 is central to the dynamics of spliceosome assembly [ 11 ]. (biomedcentral.com)
Using genome-wide approaches and quantitative behavioral assays, we show that the P-element somatic inhibitor (PSI) and its interaction with the U1 small nuclear ribonucleoprotein complex (snRNP) control male courtship behavior. (princeton.edu)
U6 small nuclear ribonucleoprotein (snRNP) biogenesis is essential for spliceosome assembly, but not well understood. (cornell.edu)

Overall, the authors see RedChIP as a straightforward and accurate means of identifying RNAs associated with genomic regions occupied by a protein of interest. (epigenie.com)

Its major distinguishing features are the ability to bind RNA in a very specific manner through two RNA recognition motifs (RRM) and the C-terminal portion of the protein, which includes a glycine-rich domain that is involved in most of the protein interactions described 3 . (nature.com)

We found both previously predicted, highly conserved residues as well as additional residues in the RBD to be essential for C1 RNA binding. (korea.ac.kr)
These results demonstrate that although the RBD is the primary determinant of C1 RNA binding, residues in the C1-C1 interaction domain also influence the RNA binding activity of the protein. (korea.ac.kr)

Alternative pre-mRNA splicing (AS) is a critical regulatory mechanism that operates extensively in the nervous system to produce diverse protein isoforms. (princeton.edu)
Studies in the past decade have demonstrated the essential roles of several AJC protein, including underlying actinomyosin components, actin isoforms, and intracellular signal transducer and activator of transcription (STAT) transcription factors, and nuclear factor-κB (NF-κB) in the maintenance and remission of IBD progression ( 5 ). (frontiersin.org)

Small heat shock protein αB-crystallin is part of cell cycle dependent Golgi reorganization J Biol Chem 2004;279:43374-7. (uclahealth.org)

To identify proteins interacting with alpha A-crystallin (CRYAA) and to investigate the potential role that these protein interactions play in the function of CRYAA using a human proteome (HuProt) microarray. (molvis.org)

3.0: hematopoietic cell-specific Lyn substrate 1 (HCLS1), Kelch domain-containing 6 (KLHDC6), sarcoglycan delta (SGCD), KIAA1706 protein (KIAA1706), RNA guanylyltransferase and 5′-phosphatase (RNGTT), chromosome 10 open reading frame 57 (C10orf57), chromosome 9 open reading frame 52 (C9orf52), and plasminogen activator, urokinase receptor (PLAUR). (molvis.org)

Cells depend on their DNA for coding information to make various classes of proteins that include enzymes, certain hormones, transport proteins, and structural proteins that support life. (cdc.gov)

miRNAs are the most well-studied small RNAs during the latest decades. (hindawi.com)
Although miRNAs have been examined in Adv type 3-infected human laryngeal epithelial cells and Adv type 2-infected human lung fibroblast cells [ 19 , 20 ], there is no research about small RNA profiling in whole blood of Adv-infected pneumonia children. (hindawi.com)

We established an efficient approach, crosslinking of segmentally isotope-labeled RNA and tandem mass spectrometry (CLIR-MS/MS), to localize protein-RNA interactions simultaneously at amino acid and nucleotide resolution. (nih.gov)

At the ribosome, the processed mRNA is translated to produce proteins from amino acid units. (cdc.gov)

Our laboratory investigates physical mechanisms in virus-host interactions that regulate the decision between latency and reactivation. (lu.se)

The X-linked tumor suppressor TSPX interacts and promotes degradation of the hepatitis B viral protein HBx via the proteasome pathway PLoS One. (usc.edu)

To this aim, several methodologies were used, such as fluorescence, surface plasmon resonance (SPR), nuclear magnetic resonance, differential scanning calorimetry, static light scattering and transmission electron microscopy. (unina.it)

A genome-wide in situ hybridization map of RNA-binding proteins reveals anatomically restricted expression in the developing mouse brain. (nih.gov)
However, few reports on genome-wide lncRNAs are available in bread wheat using high-throughput RNA sequencing. (biomedcentral.com)

Pausing and termination can be further modulated by elongation factors, such as N-utilization substances A and G. Some regulatory factors or RNAs can stably insulate RNAP from the destabilizing effects of terminators over long distances (processive anti-termination). (fu-berlin.de)
These recruitment events are inhibited in a normal wild type HSV-1 infection by the viral regulatory protein ICP0. (mdpi.com)

Anatomy11

Organisms14

Diseases4

Chemicals and Drugs92

Analytical, Diagnostic and Therapeutic Techniques and Equipment38

Phenomena and Processes84

Disciplines and Occupations5

Information Science17

Health Care5

Genes17

Ribosomal4

Complexes7

Molecules6

SnRNA2

Transcripts6

Gene expression11

Polymerases4

Polypyrimidine Tract-B1

Replication12

Molecular9

Metabolism3

Eukaryotic2

Chromatin7

Genomes3

Uridine-rich1

NcRNA4

ENCODE1

Nucleus4

RRNA1

Ribonucleoproteins1

Regulation5

Nascent1

Homolog2

Peptides5

Cellular4

Biological6

Affinity4

Viral proteins1

Mutation1

Transcriptional2

Cytoplasmic5

Sequences1

LncRNA1

Interact1

Crystallography2

Assemble3

Functional2

Nucleic acid1

Structures2

SnRNP3

Genomic1

Ability to bind1

Residues2

Isoforms2

HEAT SHOCK P1

Identify proteins1

Substrate1

Structural proteins1

MiRNAs2

Mass spectrometry1

Amino acid1

Mechanisms1

Degradation1

Magnetic Resonance1

Genome-wide2

Regulatory2