Loading...
  • eukaryotic
  • Type III secreted Sop protein effectors are delivered into target eukaryotic cells and elicit cellular responses underlying Salmonella pathogenicity. (asm.org)
  • A variety of gram-negative pathogenic bacteria possess a dedicated protein secretion system, denoted type III, which is of primary importance for the successful engagement of eukaryotic host cells (for a recent review, see reference 8 ). (asm.org)
  • Recent work by our group and others has identified a number of protein effectors ( 3 , 4 , 5 , 9 , 12 , 17 ) secreted and translocated into eukaryotic cells by the Salmonella Inv/Spa type III secretion system, which is one of two type III systems present in salmonellae (for a review, see reference 2 ). (asm.org)
  • At least some of the Sip proteins appear to have multiple activities and perform several functions, but it is believed that the major function of Sip B, C, and D is to execute the translocation of a set of specific effector proteins into eukaryotic cells. (asm.org)
  • Eukaryotic ribosomal proteins, unlike their bacterial homologues, possess nuclear localization signals (NLSs) to enter the cell nucleus during ribosome assembly. (nih.gov)
  • Here we provide a comprehensive comparison of bacterial and eukaryotic ribosomes to show that NLSs appear in conserved ribosomal proteins via remodelling of their RNA-binding domains. (nih.gov)
  • Protein uS12 is one of the 15 ribosomal proteins in which extensions have different folds in bacteria and eukaryotes, despite being bound to nearly identical rRNA cavities of bacterial and eukaryotic ribosomes. (nih.gov)
  • For this purpose, we examined how many homologous proteins have different folds of their rRNA-binding domains within similar rRNA cavities of bacterial and eukaryotic ribosomes. (nih.gov)
  • We preformed structural comparison of all homologous proteins from eukaryotic (S. cerevisiae) and bacterial (E. coli) ribosomes and, where necessary, analysed surrounding of ribosomal proteins in the ribosome structure (Supplementary Online Methods). (nih.gov)
  • Specificities
  • In order to assess the Sop protein profiles from Salmonella strains with different host specificities, we first transduced the polar sipB mutation from serovar Dublin B1 into a variety of other Salmonella strains (virulent field isolates from the IAH strain collection) by using P22 transduction. (asm.org)
  • strains
  • The deletion strains could be complemented in trans by their respective Mrp protein, but expression of MrpA in the B. subtilis Delta mrpD strain and vice versa did not improve growth at pH 7.4. (lu.se)
  • This suggested that sipB mutants of different Salmonella strains may have different profiles of Sop proteins deposited into filamentous aggregates. (asm.org)
  • The twodimensional gel images of all strains are similar and dominated by housekeeping proteins such as chaperones and metabolic enzymes. (sustech.edu)
  • cytoplasm
  • At the level of individual cell, several different WASP/WAVE family proteins are expressed together in the cytoplasm and they are thought to cooperate in cell migration by forming protrusions ( 1 , 15 - 18 ). (aacrjournals.org)
  • Type III secretion systems play a central role in virulence, directing secretion and translocation of several bacterial effector proteins into the cytoplasm of host cells. (asm.org)
  • isoforms
  • These novel findings suggest RAD51D alternative isoforms potentially regulate HR. Finally, ongoing proteomics research has identified 72 RAD51D interacting candidates that include: MCM5, Histone H2A.1, Numatrin, Nucleotide binding protein 2, and PML. (openthesis.org)
  • bacterial
  • Upon translocation, the bacterial protein effectors affect different host cell functions and elicit a variety of responses. (asm.org)
  • cDNA
  • A complementary DNA (cDNA) clone expressing RNA helicase A was isolated by screening a human cDNA library with polyclonal antibodies produced against the purified protein. (semanticscholar.org)
  • signals
  • Insights into the origin of the nuclear localization signals in conserved ribosomal proteins. (nih.gov)
  • mutant
  • SopE was initially identified as a component of protein aggregates accumulated in the culture media of Salmonella enterica serovar Dublin B1 ( sipB mutant) ( 17 ). (asm.org)
  • repeats
  • Gain-and loss-of-function mutants provided evidence for a role of SWIB5 in influencing mtDNA architecture and homologous recombination at specific intermediate-sized repeats both under normal and genotoxic conditions. (ugent.be)
  • suggests
  • The identity of SAD-6 as a Rad54 paralog suggests that this process may be similar to the searching mechanism used during homologous recombination. (genetics.org)
  • This finding enabled us to identify previously unknown NLSs in ribosomal proteins from humans, and suggests that, apart from promoting protein transport, NLSs may facilitate folding of ribosomal RNA. (nih.gov)
  • specific
  • This corroborates that the two proteins have unique specific functions. (lu.se)
  • It is becoming apparent that the mechanism of type III secretion and translocation is conserved in a variety of bacteria, although each pathogen appears to have a specific set of secreted protein effectors. (asm.org)
  • Previous studies have shown that chronic i.v. treatment with morphine or heroin decreased mu opioid receptor activation of G-proteins in specific brain regions. (semanticscholar.org)
  • processes
  • Four of the key proteins in these processes are Sip A, B, C, and D. All these proteins are encoded by a single polycistronic operon located adjacent to the inv/spa loci ( 7 , 10 , 11 , 16 ). (asm.org)
  • Indeed, these properties likely favor the formation of heteromeric associations that may be essential for the intricate regulation of the processes in which these proteins are involved. (hindawi.com)
  • distinct
  • This function appears to be distinct from the classical one performed by these proteins in HR dependent DNA Double Strand Break repair. (ox.ac.uk)
  • similar
  • The protein common interface database (ProtCID) is a database that contains clusters of similar homodimeric and heterodimeric interfaces observed in multiple crystal forms (CFs). (semanticscholar.org)
  • function
  • Can you find any clues to the function of these homologous proteins? (expertsmind.com)
  • The use of electron microscopy mediated analysis of replication intermediates is allowing the dissection of several mechanistic aspects of HR proteins function in replication fork protection. (ox.ac.uk)