AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation Science
LeechesHirudinsHirudo medicinalisThrombinLeechingAntithrombinsPartial Thromboplastin TimeFibrinopeptide AGangliaGanglia, InvertebrateHeparinFibrinopeptide BRecombinant ProteinsReceptors, ThrombinInvertebrate HormonesAntithrombin IIIAmino Acid SequenceFibrinolytic AgentsBlood Coagulation
SalivaMedicinal leechesPeptide hirudinThrombinGenetically engineeredSuppressesAnticoagulantPeptidesPreventsProteaseBloodResearchersSolubleTissueEnzymesRecombinantPurificationDisordersHealInsectCenturiesAbsorptionReactionLiveNotableWorkFindingsFoundMaskMedicalProduceMolecularMedicinePotentialGroupsCaseSuggestHumanInterest
Saliva8
  • Most patients with rickettsial diseases, such as Japanese spotted fever and scrub typhus, do not notice a tick or mite bite ( 4 ), but a leech bite is easy to detect because the site bleeds for an extended time due to hirudin in leech saliva. (cdc.gov)
  • The compound in the saliva that is the most specific for anticoagulation is the peptide hirudin. (healthwisdom.shop)
  • Leeches only have a small amount of this compound in their saliva, and there are several different forms of hirudin mixed together. (healthwisdom.shop)
  • Cancer treatments using leech therapy are being explored because of the platelet inhibitors and special enzymes contained in leech saliva. (healthline.com)
  • Animal testing also shows that directly injecting leech saliva into mice helps prevent the colonization of cancer cells. (healthline.com)
  • The Hirudin substance in leech saliva thins the blood and keeps it from clotting. (healthline.com)
  • Synthetic forms of leech saliva now exist, but researchers have discovered that using as few as four leeches in one session can help reduce the risk of amputation. (healthline.com)
  • Researchers are now studying a genetically engineered version of hirudin, a powerful blood thinner found in the saliva of the European leech Hirudo medicinalis. (theherbsplace.com)
Medicinal leeches6
  • Buy Shui Zhi 水蛭胶囊, Hirudin Capsule, Medicinal Leeches, Hirudo Medicinalis Online Direct From China.Hirudin is a compound made by medicinal leeches that interferes with the body's ability to form blood clots. (healthwisdom.shop)
  • Hirudin is a compound made by medicinal leeches that interferes with the body's ability to form blood clots. (healthwisdom.shop)
  • Medicinal leeches, Hirudo medicinalis, produce a number of compounds in their salivary glands that help with the process of bloodletting from their hosts, known as hirudotherapy. (healthwisdom.shop)
  • Our results suggest that WP-77 from W. pigra plays a distinct role in treating thrombotic diseases, and it is an essential substance of anticoagulant activity of non-suck-blood medicinal leeches. (bioinfor.com)
  • Medicinal leeches have three jaws with tiny rows of teeth. (healthline.com)
  • Medicinal leeches most often come from Hungary or Sweden. (healthline.com)
Peptide hirudin1
  • The best-known species, such as the medicinal leech, Hirudo medicinalis, are hematophagous, attaching themselves to a host with a sucker and feeding on blood, having first secreted the peptide hirudin to prevent the blood from clotting. (wikipedia.org)
Thrombin5
  • Such anticoagulation involves the inhibition of the protein thrombin, which catalyzes blood clot formation. (healthwisdom.shop)
  • This reaction is catalyzed by an enzyme called thrombin, which is a protease and can cleave other proteins. (healthwisdom.shop)
  • Hirudin inactivates only the type of thrombin that is the most specific for fibrinogen, and therefore it is the most potent compound that inhibits thrombin. (healthwisdom.shop)
  • Hirudin can act on thrombin that has formed a mesh and become a clot. (healthwisdom.shop)
  • Direct thrombin inhibitors (hirudin, lepirudin, bialirudin, argatroban, dabigatran) are expected to replace heparin but are not in widespread use. (osoms.ca)
Genetically engineered1
  • The preparation of valuable recombinant (genetically engineered) polypeptides, for example pharmaceutical proteins, relies frequently on techniques which involve the production of these polypeptides as fusion or hybrid proteins. (justia.com)
Suppresses2
  • Leech oil contains hirudin, an element that suppresses clotting in the blood and improves blood circulation. (naturessure.com)
  • Leech oil contains hirudin that suppresses blood clotting and improves blood circulation, leading to healthier scalp & hair growth. (naturessure.com)
Anticoagulant6
  • In modern times, leeches find medical use in treatment of joint diseases such as epicondylitis and osteoarthritis, extremity vein diseases, and in microsurgery, while hirudin is used as an anticoagulant drug to treat blood-clotting disorders. (wikipedia.org)
  • Several native proteins explored from suck-blood leeches, such as non-thermostable hirudin and its variants, revealed potent anticoagulant activity. (bioinfor.com)
  • We aimed to purify and characterize thermostable anticoagulant proteins from W. pigra for further development of a therapeutic agent for thrombosis. (bioinfor.com)
  • Anticoagulant proteins were purified by DEAE cellulose DE-52, Sephadex G-75, and reversed-phase liquid chromatography sequentially and analyzed by SDS-PAGE and LC-MS/MS for structural information. (bioinfor.com)
  • The anticoagulant protein WP- 77 has a relative molecular weight of ca. 20.8 k Da. (bioinfor.com)
  • This thermostable anticoagulant protein could be a promising candidate for the development of clinical antithrombosis medicines. (bioinfor.com)
Peptides3
  • However, only a few natural proteins and peptides have been identified from the fresh material of this medicinal species. (bioinfor.com)
  • This is because leeches secrete peptides and proteins that work to prevent blood clots. (healthline.com)
  • They release the proteins and peptides that thin blood and prevent clotting. (healthline.com)
Prevents1
Protease2
  • No. 4,743,679 which discloses a process for the production of human epidermal growth factor comprising cleavage of a fusion protein by Staphylococcus aureus V8 protease. (justia.com)
  • And I would like to show that with a work that I was involved in with Michel Ehrmann and Tim Clausen, where we had determined the structure of a intracellular protease and chaperone called DegQ, a ubiquitous protein with some variations. (lindau-nobel.org)
Blood18
  • Leeches have been used in medicine from ancient times until the 19th century to draw blood from patients. (wikipedia.org)
  • Urinalysis was positive for protein and occult blood. (cdc.gov)
  • The use of live leeches risks the introduction of bacteria into the wound, and hirudin is generally used by itself to prevent blood from clotting. (healthwisdom.shop)
  • Also in the mixture are compounds that keep the blood from clotting, so it will flow more freely into the leech. (healthwisdom.shop)
  • The blood continues to remain unclotted, even after the leech has detached. (healthwisdom.shop)
  • Blood clotting involves the conversion of soluble protein called fibrinogen to the insoluble mesh fibrin. (healthwisdom.shop)
  • Traditional Chinese medicine clinics have proved that non-suck-blood leech Whitmania pigra Whitman ( W. pigra ) also played notable roles in CVD treatments even after decoction. (bioinfor.com)
  • A leech can be used to heal 15 million blood. (read.cash)
  • So 'Hirudin', 'Calicrain', there are some sources like 'Calyn' which helps to remove the naughty blood. (read.cash)
  • The leeches are then allowed to extract blood, for 20 to 45 minutes at a time, from the person undergoing treatment. (healthline.com)
  • This equates to a relatively small amount of blood, up to 15 milliliters per leech. (healthline.com)
  • People with anemia, blood clotting conditions, or compromised arteries are not candidates for leech therapy. (healthline.com)
  • During a session, live leeches attach themselves to the target area and draw blood. (healthline.com)
  • Leeches are effective at increasing blood circulation and breaking up blood clots. (healthline.com)
  • People with heart disease use leech therapy because of its potential to improve inflammation and blood flow. (healthline.com)
  • While people with certain blood cancers are not advised to use leech therapy, it has been shown to slow the effects of lung cancer. (healthline.com)
  • Since people with diabetes tend to have thicker blood, Hirudin can help relieve the pressure on the heart and cardiovascular system by thinning the blood. (healthline.com)
  • In nature, anticoagulants are present in haemovores (i.e. an animal that primarily feeds on blood) such as leeches. (osoms.ca)
Researchers1
  • Researchers have observed positive outcomes in cases where Hirudin has been used for treat diabetes. (healthline.com)
Soluble1
  • Secondly, those proteins which are present in soluble form in the cytoplasm often are subject to degradation by host specific enzymes, thus reducing the amounts of active protein that can be recovered. (justia.com)
Tissue2
  • In leech, the ______ tissue lies beneath longitudinal muscles and fills the entire colon around the gut. (samacheerkalvibook.com)
  • Leeches have become popular for preserving soft tissue and promoting healing after facial reconstructive surgery. (healthline.com)
Enzymes1
  • Enzymes known to the prior art as "proteolytic enzymes" have been found to be particularly well suited for the cleavage of fusion proteins. (justia.com)
Recombinant3
  • The method involves expressing the recombinant polypeptide as a fusion protein with a pro-peptide. (justia.com)
  • The pro-peptide-polypeptide fusion protein can be cleaved and the recombinant polypeptide released under the appropriate conditions. (justia.com)
  • Their preliminary findings suggest the clot-blocking prowess of the recombinant version may rival that of natural hirudin. (theherbsplace.com)
Purification1
  • Conversion of this insoluble material involves often slow and complex refolding methods, making protein purification difficult. (justia.com)
Disorders1
  • In the past few years, leech therapy has become an acceptable alternative therapy for people with vascular disease and disorders. (healthline.com)
Heal1
  • The leeches leave behind small, Y-shaped wounds that usually heal without leaving a scar. (healthline.com)
Insect2
  • Bacillus thuringiensis, is a naturally occurring soil bacterium that produces a crystal (Cry) protein that is toxic to insect larvae. (biologydiscussion.com)
  • I started in '68 working on a red insect protein and surprisingly found out that it is related the whale myoglobin - first example of molecular evolution by structure determination. (lindau-nobel.org)
Centuries2
Absorption1
  • Low molecular weight heparin (LMWH) has better absorption after subcutaneous administration, and reduced protein binding results in improved bioavailability. (gponline.com)
Reaction1
  • The cleavage reaction is performed by contacting the fusion protein with a proteolytic enzyme under appropriate conditions. (justia.com)
Live2
  • The majority of leeches live in freshwater habitats, while some species can be found in terrestrial or marine environments. (wikipedia.org)
  • There are some disadvantages to using live leeches for anticoagulation, since they can introduce bacteria into the wound. (healthwisdom.shop)
Notable1
  • Notable laboratory data included low platelet count (102,000/µL), slightly elevated aspartate aminotransferase (52 IU/L), elevated lactate dehydrogenase (468 IU/L), and elevated C-reactive protein (5.37 mg/dL). (cdc.gov)
Work2
Findings1
  • But new scientific findings hint that physicians may once again find value in the lowly leech. (theherbsplace.com)
Found4
  • Except for Antarctica, leeches are found throughout the world but are at their most abundant in temperate lakes and ponds in the northern hemisphere. (wikipedia.org)
  • the element -bdella found in many leech group names is from the Greek βδέλλα bdella, also meaning leech. (wikipedia.org)
  • Despite the additional steps which are required to produce a protein of interest as a fusion protein, rather than directly in its active form, the production of hybrid proteins has been found to overcome a number of problems. (justia.com)
  • founded a school, by the way, in Cambridge, England, where Max Perutz worked, the father of protein crystallography, who found a way to decipher the complex diffraction patterns of proteins. (lindau-nobel.org)
Mask1
  • In addition, eggs give your hair a protein-rich mask, which makes your hair stronger, healthier, and longer. (naturessure.com)
Medical1
  • By the early 20th century, leeches had lost their grip on medical practice as doctors realized that bloodletting usually did more harm than good. (theherbsplace.com)
Produce4
  • They produce anesthetics, so the host will be less likely to notice as the leech attaches to them. (healthwisdom.shop)
  • Crystal protein genes have been transferred into corn, enabling the corn to produce its own pesticides against insects such as the European corn borer. (biologydiscussion.com)
  • Transgenic Rice and transgenic Potato produce respectively higher content of vitamin A and protein. (biologydiscussion.com)
  • One such haircare secret is Hirudin-enriched oil that is known to produce unbelievable results in managing baldness & alopecia. (naturessure.com)
Molecular1
  • The phylogenetic tree of the leeches and their annelid relatives is based on molecular analysis (2019) of DNA sequences. (wikipedia.org)
Medicine2
  • Since the time of ancient Egypt, leeches have been used in medicine to treat nervous system abnormalities, dental problems, skin diseases, and infections. (healthline.com)
  • A recent case study showed how traditional Unani medicine, which includes leech therapy, was able to help save the foot of a 60-year-old woman with diabetes. (healthline.com)
Potential1
  • The land leech is a potential vector of R. japonica . (cdc.gov)
Groups1
  • Both groups are hermaphrodites and have a clitellum, but leeches typically differ from the oligochaetes in having suckers at both ends and in having ring markings that do not correspond with their internal segmentation. (wikipedia.org)
Case1
  • We describe a case of Japanese spotted fever after a land leech ( Haemadipsa zeylanica japonica ) bite. (cdc.gov)
Suggest1
  • Clinical trials suggest that leech therapy is an appropriate treatment for the common joint disease osteoarthritis . (healthline.com)
Human1
  • A protein called 'Destabilize' from the body of a leech and enters into the human body which kill a lot of dogged virus. (read.cash)
Interest1
  • All of these procedures result in the production a hybrid protein in which the protein of interest is linked to an additional polypeptide. (justia.com)