• Lampe D.J., Willis J.H. (1994) Characterization of a cDNA and gene encoding a cuticular protein from rigid cuticles of the giant silkmoth, Hyalophora cecropia , Insect Biochem. (apidologie.org)
  • Here we present the first characterization of the genome ofan entomopoxvirus (EPV) which infects the North American migratorygrasshopper Melanoplus sanguinipes and other important orthopteran pests.The 236-kbp M. sanguinipes EPV (MsEPV) genome consists of a central codingregion bounded by 7-kbp inverted terminal repeats and contains 267 openreading frames (ORFs), of which 107 exhibit similarity to previouslydescribed genes. (embl-heidelberg.de)
  • Characterization of cytochrome P450 genes involving in ecdysteroidogenesis in silkworm (Bombyx mori). (chinaagrisci.com)
  • Identification and Characterization of Genes Involved in Ecdysteroid Esterification Pathway Contributing to the High 20-Hydroxyecdysone Resistance ofHelicoverpa armigera. (cqu.edu.cn)
  • Functional characterization of the horizontally transferred 4,5-DOPA extradiol dioxygenase gene in the domestic silkworm, Bombyx mori. (cqu.edu.cn)
  • Identification of two isoforms of Pop in the domestic silkworm, Bombyx mori: Cloning, characterization and expression analysis. (cqu.edu.cn)
  • development and characterization of a new bombyx mori cell line for protein expression, journal of asia-pacific entomology. (ijust18.com)
  • Asano T., Ashida M. (2001) Cuticular pro-phenoloxidase of the silkworm, Bombyx mori . (apidologie.org)
  • Nakato H., Toriyama M., Izumi S., Tomino S. (1990) Structure and expression of mRNA for a pupal cuticle protein of the silkworm, Bombyx mori , Insect Biochem. (apidologie.org)
  • Izumi S., Tomino S. (1994) Structure and developmental expression of a larval cuticle protein gene of the silkworm, Bombyx mori , Biochim. (apidologie.org)
  • We found the activity of arylsulfatase in the midgut contents of the silkworm, Bombyx mori . (go.jp)
  • GATA-type zinc finger motif-containing sequences and chorion gene transcription factors of the silkworm Bombyx mori. (expasy.org)
  • 2019.2.23, Our paper " High-quality genome assembly of the silkworm, Bombyx mori " was published. (u-tokyo.ac.jp)
  • Comparative analysis of iTRAQ-based proteomes for cocoons between the domestic silkworm (Bombyx mori) and wild silkworm (Bombyx mandarina). (cqu.edu.cn)
  • Transcription factor E74A affects the ecdysone titer by regulating the expression of the EO gene in the silkworm, Bombyx mori. (cqu.edu.cn)
  • A. M. Khurad, A. Mahulikar, M. K. Rathod, M. M. Rai, S. Kanginakudru, Nagaraju J. (2004) Vertical transmission of nu-cleopolyhedrovirus in the silkworm, Bombyx mori L. Journal of Invertebrate Pathology 87, 8-15. (scirp.org)
  • Additional studies have also shown that hemolymph from the silkworm ( Bombyx mori ) can inhibit insect and mammalian cell apoptosis that is induced by viruses and several chemical inducers, such as staurosporine, camptothecin, and actinomycin D [ 6 - 8 ]. (hindawi.com)
  • Screening and Identification of Candidate Proteins Interacting with BmHSP60 in the Silkworm ( Bombyx mori )[J]. Scientia Agricultura Sinica, 2019, 52(2): 376-384. (chinaagrisci.com)
  • Prophenoloxidase-activating enzyme of the silkworm, Bombyx mori. (lookformedical.com)
  • Prophenoloxidase-activating enzyme (PPAE) was purified to homogeneity as judged by SDS-polyacrylamide gel electrophoresis from larval cuticles of the silkworm, Bombyx mori. (lookformedical.com)
  • Several genes were present as members of gene families, as has also been observed in other insect species. (biomedcentral.com)
  • With reference to insect immunity, the ESTs have been obtained from baculovirus-infected B. mori cultured cells and pupae, but no large scale information on bacteria-induced immune genes is as yet available. (biomedcentral.com)
  • Binger L.C., Willis J.H. (1994) Identification of the cDNA, gene and promoter for a major protein from flexible cuticles of the giant silkmoth Hyalophora cecropia , Insect Biochem. (apidologie.org)
  • Charles J.-P., Chihara C., Nejad S., Riddiford L.M. (1998) Identification of proteins and developmental expression of RNAs encoded by the 65A cuticle protein gene cluster in Drosophila melanogaster , Insect Biochem. (apidologie.org)
  • Cox D.C., Willis J.H. (1985) The cuticular proteins of Hyalophora cecropia from different anatomical regions and metamorphic stages, Insect Biochem. (apidologie.org)
  • Dotson E.M., Cornel A.J., Willis J.H., Collins F.H. (1998) A family of pupal-specific cuticular protein genes in the mosquito Anopheles gambiae , Insect Biochem. (apidologie.org)
  • Palli S.R., Locke M. (1987) The synthesis of hemolymph-proteins by the larval epidermis of an insect Calpodes ethlius (Lepidoptera, Hesperiidae), Insect Biochem. (apidologie.org)
  • Rebers J.E., Niu J., Riddiford L.M. (1997) Structure and spatial expression of the Manduca sexta MSCP14.6 cuticle gene, Insect Biochem. (apidologie.org)
  • Santos A.E., Bitondi M.M.G., Simões Z.L.P. (2001) Hormone-dependent protein patterns in integument and cuticular pigmentation in Apis mellifera during pharate adult development, J. Insect Physiol. (apidologie.org)
  • As part of our efforts to develop a strategy that results in engineered male-sterile strains with minimum effects on viability and mating competition, we used CRISPR/Cas9 technology to disrupt Ser2, which encodes a seminal fluid protein, in the model lepidopteran insect, Bombyx mori, and an important agricultural pest, Plutella xylostella. (ox.ac.uk)
  • Objective】 HSP60 is an important member of heat shock proteins, which plays an important role in the innate immunity of insects, and is also an essential factor for insect growth and development. (chinaagrisci.com)
  • Here, we report that the recently sequenced genome from the red flour beetle Tribolium castaneum contains a gene coding for an oxytocin/vasopressin-like peptide, identical to the locust peptide, which we named inotocin (for insect oxytocin/vasopressin-like peptide) and a gene coding for an inotocin G protein-coupled. (lu.se)
  • The inotocin/receptor couple can also be found in the recently sequenced genome from the parasitic wasp Nasonia vitripennis but not in any other holometabolous insect with a completely sequenced genome (12 Drosophila species, the malaria mosquito Anopheles gambiae, the yellow fever mosquito Aedes aegypti, the silk worm Bombyx mori, and the honey bee Apis mellifera), suggesting that this neuropeptide system is confined to basal holometabolous insects. (lu.se)
  • To characterize the proteins involved in cell clump/cell adhesion of insect cellular defense reactions, we induced the cell clump/cell adhesion reaction in vitro with the hemolymph of larvae of the coleopteran insect, Tenebrio molitor. (lookformedical.com)
  • In this study, we characterized the small subunit of ubiquinol-cytochrome C reductase (Bmuccr) of the silkwormBombyx mori, a model insect of Lepidopteron species. (researchgate.net)
  • Expression of partial sequences of BRO-A proved that theN-terminal 80 amino acid residues were required for DNA binding activity.We also demonstrated that BmNPV BRO proteins underwent phosphorylation andubiquitination followed by proteasome degradation, which may explain theirdistribution in the cytoplasm as well as the nucleus. (embl-heidelberg.de)
  • For expressing foreign genes, a new host- vector system, with Bombyx mori silkworm larvae as the host and B. mori nuclear polyhedrosis virus (BmNPV) as the vector, has been developed. (microbiologyresearch.org)
  • BmNPV genomic DNA codes polyhedrin which is a major protein of inclusion bodies and is mass- produced in infected silkworm larvae. (microbiologyresearch.org)
  • S. Gomi, C. E. Zhou, W. Y. Yih, K. Majima, Maeda S. (1997) Deletion analysis of four of eighteen late gene expression factor gene homologues of the baculovirus, BmNPV. (scirp.org)
  • The silkworm larvae infected with the recombinant virus produced 3·6 mg of the fusion protein per larva and the infected BM-N cells produced 0·3 mg per ml of culture. (microbiologyresearch.org)
  • 107 and 110 varieties showed the highest expression of HSP70 gene (552 and 53) among female and male silkworm larvae groups, respectively. (ac.ir)
  • And the expression of BmSPI39 in response to B. bassiana invasion in the integument of B. mori larvae and pupae at different time after B. bassiana infection was assayed using the prepared BmSPI39 antibodies by Western blot. (insect.org.cn)
  • The results of Western blot showed that the expression of BmSPI39 in the integument of B. mori larvae was up-regulated at 84 h and down-regulated at 108 and 120 h after B. bassiana infection. (insect.org.cn)
  • The typical Bro proteins that have been experimentally investigated are BroA, BroC and BroD from Bombyx mori uclear polyhedrosis virus (BmNV). (embl-heidelberg.de)
  • The coding sequences of the hepatitis B virus surface antigen were inserted into a baculovirus transfer vector produced from Autographa califomica nuclear polyhedrosis virus (AcNPV) so that the foreign gene was under the control of the AcNPV polyhedrin promoter. (microbiologyresearch.org)
  • E. A. van Strien, D. Zuidema, R.W. Goldbach, Vlak, J. M. (1992) Nucleotide sequence and transcriptional analysis of the polyhedrin gene of Spodoptera exigua nuclear polyhedrosis vi-rus. (scirp.org)
  • B. mori nuclear polyhedrosis virus (bacmid, conserved by our lab) was propagated in BmN cells. (hindawi.com)
  • G4 and i-motif structures were identified in the promoter of the transcription factor gene BmPOUM2 , which regulates the expression of the wing disc cuticle protein gene (BmWCP4) during metamorphosis in Bombyx mori . (isocce.org)
  • The reports on base sequences of spider silk protein genes have gained importance as the mechanical properties of silk fibers have been revealed. (bioone.org)
  • Silks are composed principally of proteins with a predominance of alanine, serine and glycine and silk proteins are able to undergo irreversible transformations from soluble protein to insoluble fibres. (bioone.org)
  • We utilized the YI , Y+ I , + Y I and + Y + I mutants of Bombyx mori to characterize lipid absorption and transport from midgut to silk glands via lipophorin. (confex.com)
  • A carotenoid binding protein (CBP) has been isolated from the silk gland of Bombyx mori . (confex.com)
  • Transcriptional data suggest a specific expression of the gene in middle silk glands. (go.jp)
  • Aqueous silk fibroin derived from Philosamia ricini (PR), Antheraea assamensis (AA), and Bombyx mori (BM) were cast as approximately 15 µm films with and without pores on which human CE cells were cultured. (arvojournals.org)
  • A third of the transcriptome showed similarity with previously characterized immune-related genes that included both the known and putative immune genes. (biomedcentral.com)
  • Of the four putative novel defence proteins (DFPs) annotated by PSI-BLAST three showed similarity to extracellular matrix proteins from vertebrates implicated in innate immunity, while the fourth was similar to, yet distinct from, the anti-microbial protein cecropin. (biomedcentral.com)
  • The present study has identified many potential immune-related genes in A. mylitta some of which are vertebrate homologues and others are hitherto unreported putative defence proteins. (biomedcentral.com)
  • Testing of proteins for auto-ubiquitinylation activity allowing their identification as putative ubiquitin E3 ligases. (enzolifesciences.com)
  • Utilizing the first three steps in the ubiquitin cascade the kit facilitates ubiquitinylation of known or putative E3 ligase enzymes followed by Western blot analysis using the highly sensitive reagents provided or using antibodies to the specific protein of interest (user supplied). (enzolifesciences.com)
  • MsEPV genes with putative functions in preventionand repair of DNA damage include a complete base excision repair pathway(uracil DNA glycosylase, AP endonuclease, DNA polymerase beta, and anNAD+-dependent DNA ligase), a photoreactivation repair pathway(cyclobutane pyrimidine dimer photolyase), a LINE-type reversetranscriptase, and a mutT homologue. (embl-heidelberg.de)
  • 2022.6.7, Our paper " SilkBase: an integrated transcriptomic and genomic database for Bombyx mori and related species " was published. (u-tokyo.ac.jp)
  • This great importance necessitates a better understanding of key biological processes of crustaceans in order to address key bottlenecks in the fishery and aquaculture industries to enable it to meet the ever-growing demand in quality protein [ 6 ], as well as to devise species-specific treatments for invasive species. (biomedcentral.com)
  • Hiruma K., Hardie J., Riddiford L.M. (1991) Hormonal regulation of epidermal metamorphosis in vitro: control of expression of a larval-specific cuticle gene, Dev. (apidologie.org)
  • Kiely M.L., Riddiford L.M. (1985) Temporal programming of epidermal cell protein synthesis during the larval-pupal transformation of Manduca sexta , Wilhelm Roux's, Arch. (apidologie.org)
  • Ochieng V.O., Osir E.O., Ochanda J.O., Olembo N.K. (1993) Temporal synthesis of cuticle proteins during larval development in Glossina morsitans , Comp. (apidologie.org)
  • A single transcript chosen for a gene which is the most conserved, most highly expressed, has the longest coding sequence and is represented in other key resources, such as NCBI and UniProt. (ensembl.org)
  • Furthermore, by fitting the deduced amino acid sequence of the protein VP5 to 120 large protrusion proteins on the CPV capsid shell, we confirmed that this protrusion protein is encoded by CPV RNA segment 7. (rcsb.org)
  • Based on its partial amino acid sequence, we searched for its coding gene using Basic Local Alignment Search Tool (BLAST) and identified KWMTBOMO05106 . (go.jp)
  • Morphological transformation studies have failed to demonstrate a viral oncogene, a virus-coded transforming protein or any sequence of DNA that uniquely transforms cells according to one-hit kinetics. (microbiologyresearch.org)
  • IGF II was released from the fusion protein produced by BM-N cells infected with the recombinant virus by CNBr treatment, purified by extraction with guanidine-HCl, column chromatography and HPLC and the correct amino-terminal amino acid sequence confirmed. (microbiologyresearch.org)
  • The GATA family of transcription factors are proteins that bind to DNA sites with the consensus sequence (A/T)GATA(A/G), found within the regulatory region of a number of genes. (expasy.org)
  • W. B. Wang, S. Y. Zhu, L. Q. Wang, F. Yu, Shen W. D. (2005) Cloning and sequence analysis of the Antheraea pernyi nucleo-polyhedrovirus gp64 gene. (scirp.org)
  • The smaller replication proteins, Rep40 and Rep52, require ATP to bind DNA, show no sequence specificity in DNA binding and lack the N-terminal nuclease domains. (utoledo.edu)
  • according to morphological, physiological and its 16S rRNA gene sequence. (researchgate.net)
  • 2017.4.21, The data of the new high-quality genome assembly of Bombyx mori is now downloadable from here . (u-tokyo.ac.jp)
  • 2017.1.13, New chromosome-level genome assembly of Bombyx mori (sequencing was performed on the PacBio RS II and the Illumina HiSeq 2500) was available in SilkBase. (u-tokyo.ac.jp)
  • Chitin, the second most abundant carbohydrate in nature following cellulose, is the major component of the exoskeleton, providing a scaffold for cuticular proteins that form links between the chitin fibers, as well as stabilize deposition of minerals that harden the cuticle. (biomedcentral.com)
  • Apple R.T., Fristrom J.W. (1991) 20-hydroxyecdys-one is required for, and negatively regulates, transcription of Drosophila pupal cuticle protein genes, Dev. (apidologie.org)
  • Drosophila protein pannier (or DGATAa) (gene pnr) which acts as a repressor of the achaete-scute complex (as-c). (expasy.org)
  • Drosophila box A-binding factor (ABF) (also known as protein serpent (gene srp)) which may function as a transcriptional activator protein and may play a key role in the organogenesis of the fat body. (expasy.org)
  • The Drosophila disembodied gene controls late embryonic morphogenesis and codes for a cytochrome P450 enzyme that regulates embryonic ecdysone levels. (chinaagrisci.com)
  • Mutation of the seminal protease gene, serine protease 2, results in male sterility in diverse lepidopterans. (ox.ac.uk)
  • In the present study we constructed and analysed the immune transcriptome of the lepidopteran Antheraea mylitta , an economically important Indian tasar silkmoth with a view to unravel the potential immune-related genes and pathways. (biomedcentral.com)
  • Based on the open database SilkDB 3.0 of B. mori , the spatiotemporal expression characteristics of BmSPI39 in the immune-related tissues, integument, midgut and fat body, of B. mori from the day-3 4th instar larva to 1 day-old pupa were analyzed. (insect.org.cn)
  • The heat map analysis of the expression data of BmSPI39 showed that BmSPI39 was expressed in the integument of B. mori at the wandering stage and the pre-pupal stage, with a higher expression level in the fat body at the pre-pupal stage, but not in the midgut at all the tested developmental stages. (insect.org.cn)
  • Identification of genes involved in sex pheromone biosynthesis and metabolic pathway in the Chinese oak silkworm, Antheraea pernyi. (cqu.edu.cn)
  • They contain distinct amino- and carboxy-terminal domains (Bro-N and Bro-C, respectively) that are present independently of each other and in distinct contexts in a variety of other viral proteins. (embl-heidelberg.de)
  • Although their functions are unknown, their amino acidsequences contain a motif found in all known viral and prokaryoticsingle-stranded DNA binding proteins. (embl-heidelberg.de)
  • The coding region of the carboxy- terminal half of polyhedrin was removed and the remainder was ligated with the IGF II gene in phase to create a fusion protein gene consisting of the coding region of the amino-terminal half of polyhedrin and the IGF II gene. (microbiologyresearch.org)
  • Prediction of protein antigenic determinants from amino acid sequences. (scirp.org)
  • Aminoacyl tRNA synthetases are a class of enzymes that ensure the fidelity of protein synthesis by attaching amino acids to their cognate tRNAs. (utoledo.edu)
  • Corresponding expression changes of these proteins at different developmental stages of female ticks are very important to ensure the orderly development of the organ. (biomedcentral.com)
  • In insects, ecdysteroids are synthesized by genes of the Halloween family and play important roles in several key developmental events, including molting and metamorphosis. (chinaagrisci.com)
  • In this study, the expression patterns of the two Halloween genes AhCYP307A2 and AhCYP314A1 were determined by quantitative PCR (qPCR) at different developmental stages. (chinaagrisci.com)
  • The objective of this study is to identify the interaction proteins of BmHSP60, and to provide a basis for further exploring the mechanism in the innate immunity of B. mori . (chinaagrisci.com)
  • Expression of the fungal-resistance factor BmSPI39 in Bombyx mori in response to Beauveria bassiana invasion [J].Acta Entomologica Sinica, 2021, 64(1): 61-69. (insect.org.cn)
  • gene cloning and expression analysis of the magpx family in morus alba l .acta botanica boreali-occidentalia sinica, 2019,3(6): 0991-1000. (ijust18.com)
  • Finally, we analysed the expression profiles of 15 potential immune-related genes, and the majority of them were induced more prominently with E. coli compared to Micrococcus luteus . (biomedcentral.com)
  • Nakato H., Izumi S., Tomino S. (1992) Structure and expression of gene coding for a pupal cuticle protein of Bombyx mori , Biochim. (apidologie.org)
  • The expression of the genes encoding olfactory receptors and so on was over- or mis-expressed in sensory organs of the mutants. (nii.ac.jp)
  • Notably, 3D20E transfer also induces expression of a reproductive gene that preserves egg development during Plasmodium infection, ensuring fitness of infected females. (nature.com)
  • The expression levels of a large number of proteins during the development and degeneration of salivary glands change, which regulate the biological functions of the salivary glands. (biomedcentral.com)
  • The results showed that the expression of different types of proteins change in different characteristics in salivary glands during the unfed to engorged process of female ticks. (biomedcentral.com)
  • GATA-2 [ 2 ], a transcriptional activator which regulates endothelin-1 gene expression in endothelial cells. (expasy.org)
  • Bombyx mori BCFI [ 5 ], which regulates the expression of chorion genes. (expasy.org)
  • Neurospora crassa nit-2 [ 9 ], a transcriptional activator which turns on the expression of genes coding for enzymes required for the use of a variety of secondary nitrogen sources, during conditions of nitrogen limitation. (expasy.org)
  • Neurospora crassa white collar proteins 1 and 2 (WC-1 and WC-2), which control expression of light-regulated genes. (expasy.org)
  • Cloning of the GATA-binding protein that regulates endothelin-1 gene expression in endothelial cells. (expasy.org)
  • Human GATA-3: a lineage-restricted transcription factor that regulates the expression of the T cell receptor alpha gene. (expasy.org)
  • Animal steroid hormones initiate signaling by passive diffusion into cells and binding to their nuclear receptors to regulate gene expression. (biologists.com)
  • Aim】 To prepare polyclonal antibody of the fungal-resistance factor BmSPI39 in Bombyx mori and to analyze its expression in response to Beauveria bassiana invasion. (insect.org.cn)
  • Methods】 Prokaryotic expression and immobilized nickel ion affinity chromatography were used to obtain the highly-purified recombinant BmSPI39 protein. (insect.org.cn)
  • expression and function analysis of mulberry osmotin gene. (ijust18.com)
  • Expression of the polh gene of AnpeNPV in E. coli was examined by SDS-PAGE, Western blot and Mass-spectrum analysis. (scirp.org)
  • The result showed that the bacterium expression system was suitable for the virus gene expression. (scirp.org)
  • In the current study, we cloned and expressed the gene encoding silkworm SP2 ( sp2 ) both in a prokaryotic expression system and a silkworm baculovirus system. (hindawi.com)
  • The BmHSP60 candidate interaction protein clone expression vector was constructed and fused with HA tag, and co-transfected with BmHSP60 into B. mori cells. (chinaagrisci.com)
  • They found that PGN from Escherichia coli stimulated the expression of several antibacterial peptide genes and other genes, whereas PGN from Micrococcus luteus activated a few genes. (encyclopedia.pub)
  • They showed that E. coli PGN or cells elicited a higher expression of the peptidoglycan recognition protein gene involved in the prophenoloxidase activation pathway compared to M. luteus PGN or cells. (encyclopedia.pub)
  • Expression of CE markers was determined at the gene and protein levels using PCR and immunostaining, respectively. (arvojournals.org)
  • For example, we found that expression of TK1 , a potential target gene of a MYBL2-linked enhancer, is associated with poor patient survival. (prolekarniky.cz)
  • Finally, the cloning vector of candidate interaction proteins was constructed and fused with HA tag, and transferred into B. mori cells with BmHSP60, respectively. (chinaagrisci.com)
  • Laemmli U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227, 680-685. (apidologie.org)
  • Case studies are presented to validate the method, yielding protein designs specifically focused on structural materials, but also exploring the applicability in the design of soluble, antimicrobial biomaterials. (aip.org)
  • The nucleotide composition was obviously AT-biased, and all protein-coding genes, except for the cox1 gene (CGA), used ATN as the start codon. (authorea.com)
  • The constituent proteins are normally synthesised in specialised glands where the epithelial cells are responsible for the biosynthesis. (bioone.org)
  • Ustilago maydis urbs1 [ 7 ], a protein involved in the repression of the biosynthesis of siderophores. (expasy.org)
  • The dynamic landscape of gene regulation during Bombyx mori oogenesis. (cqu.edu.cn)
  • The results of this study provide new insights into the molecular mechanism of the secondary structures in epigenetic regulation of gene transcription. (isocce.org)
  • GATA-3 [ 3 ], a transcriptional activator which binds to the enhancer of the T-cell receptor α and delta genes. (expasy.org)
  • Caenorhabditis elegans elt-1 and elt-2, transcriptional activators of genes containing the GATA region, including vitellogenin genes [ 6 ]. (expasy.org)
  • Except for trnS1, which lacked the DHU arm, all tRNA genes could fold into the clover-leaf structure. (authorea.com)
  • Studies of the thermodynamics of binding of ligands to glycyl tRNAs suggest that the formation of glycyl adenylate, an obligate intermediate on the reaction pathway, is accompanied by a significant conformation change in the protein that alters the affinity of the enzyme for tRNA. (utoledo.edu)
  • This family includes the N-terminus of baculovirus BRO and ALI motif proteins. (embl-heidelberg.de)
  • The baculovirus Bro proteins are encoded by a multigene family. (embl-heidelberg.de)
  • The polyhedrin (polh) gene is often used to analyse evolution of baculovirus. (scirp.org)
  • In addition, the expressed protein in the silkworm baculovirus system was used to study the anti-apoptotic effects of SP2 on human umbilical vein ECs (HUVECs) induced by peroxidation. (hindawi.com)
  • Recently, the entire genome of this ectoparasitoid wasp was sequenced, enabling the identification of 79 venom proteins. (mdpi.com)
  • 2014). Where annotations were not present or were different, the online Softberry server (http://linux1.softberry.com/berry.phtml) was used to predict mRNA and protein sequences using the FGNESH+ prediction algorithm (Solovyev et al. (molquest.com)
  • The presence of genes not previously described inpoxviruses, and in some cases in any other known virus, suggestssignificant viral adaptation to the arthropod host and the externalenvironment. (embl-heidelberg.de)
  • The larger Rep proteins, Rep78 and Rep68, assemble into stable, hexameric oligomers on specific secondary structures on the 3' and 5' termini of the single stranded viral DNA. (utoledo.edu)
  • [ 1 ] reported that bacterial peptidoglycans (PGN) were more potent activators of the antimicrobial protein (AMP) gene cecropin B (CecB) than lipopolysaccharides (LPS) in the Bombyx mori cell line NISES-BoMo-Cam1. (encyclopedia.pub)
  • As one such antiapoptotic component in silkworm hemolymph, the 30 K protein has been studied widely [ 6 , 7 ]. (hindawi.com)
  • A recent study showed that another protein in silkworm hemolymph, storage protein 2 (SP2), can also inhibit staurosporine-induced HeLa cell apoptosis and ROS generation [ 8 ]. (hindawi.com)
  • Genes predicting interactions with host cellular mechanismsinclude homologues of the inhibitor of apoptosis protein, stress responseprotein phosphatase 2C, extracellular matrixin metalloproteases,ubiquitin, calcium binding EF-hand protein, glycosyltransferase, and atriacylglyceride lipase. (embl-heidelberg.de)
  • In this study, the sp2 gene was cloned and expressed in Escherichia coli to produce a 6xHis-tagged fusion protein, which was then used to generate a polyclonal antibody. (hindawi.com)
  • Results】 The results of in-gel activity staining showed that the recombinant BmSPI39 protein could strongly inhibit the activity of subtilisin A. Enzyme-linked immunosorbent assay (ELISA) showed that the antibody titer of BmSPI39 antiserum reached 1∶128 000. (insect.org.cn)
  • We used iTRAQ quantitative proteomics to study the dynamic changes in salivary gland proteins in female Haemaphysalis longicornis at four feeding stages: unfed, partially fed, semi-engorged and engorged. (biomedcentral.com)
  • In molecular biology, the protein domain WHEP-TRS refers to helix-turn-helix domains. (wikipedia.org)
  • Furthermore, to the best of our knowledge, there are only a few reports on the role of molecular motor and TCA cycle-related proteins in the salivary glands of ticks. (biomedcentral.com)
  • Using bioinformatics methods to analyze the dynamic changes of a large number of proteins, we found that molecular motor and TCA cycle-related proteins play an important role in the physiological changes of the salivary glands. (biomedcentral.com)
  • By analyzing these changes, some proteins, such as molecular motor and TCA cycle-related proteins, were screened and RNAi carried out. (biomedcentral.com)
  • The 5 bands were mixed into a protein pool for mass spectrometry, and 5 interaction proteins were screened consistent with the differential bands in silver staining according to the number of peptides and molecular weight of proteins. (chinaagrisci.com)
  • The research of my laboratory is focused on nucleic acid enzymology, nucleic acid protein interaction and more recently on the development of modified human albumin for the treatment of shock. (utoledo.edu)
  • This fusion protein gene was combined in a plasmid with the promoter and 5′ and 3′ flanking regions of the polyhedrin gene. (microbiologyresearch.org)
  • R. D. Possee, S. C. Howard, (1987) Analysis of the polyhedrin gene promoter of the Autographa californica nuclear polyhedro-sis virus. (scirp.org)
  • The latex of mulberry contains sugar-mimic alkaloidal toxins, and the mulberry feeders (Bombyx etc.) must overcome the toxicity. (nii.ac.jp)
  • The polyphagous mutants, Bt and spli were analyzed to understand the genetic basis of mulberry feeding in Bombyx. (nii.ac.jp)
  • On the other hand, we found that the major sucrases were enzymatically more tolerant to the mulberry alkaloids in Bombyx than in non-mulberry bombycoids. (nii.ac.jp)
  • technology for mulberry leaf protein extraction and protein powder preparation. (ijust18.com)
  • The results of Western blot and in-gel activity staining showed that the purified BmSPI39 antibody could effectively bind to the BmSPI39 antigen protein in different multimerization states. (insect.org.cn)
  • The most persuasive roles for HSV and HCMV in human tumourigenesis are as mutagens, as activators of cellular transcription or in switching on the synthesis of host cell proteins not normally expressed in untransformed cells. (microbiologyresearch.org)
  • We found that a transcription factor gene (Bmacj6) was defective in the mutants. (nii.ac.jp)
  • Knockdown experiments of FOXM1 and MYBL2 suggest that these factors regulate genes involved in controlling cell cycle progression and cell division. (prolekarniky.cz)
  • This work will lay a foundation for the development and utilization of protein drugs from economically important insects for treatment of vascular diseases. (hindawi.com)