Protein genes enhances protein expression. Medical search. Frequent questions

Specifically, we aim to 1) engineer organisms that are uniquely tolerant to reaction conditions, 2) use protein engineering and synthetic biology tools to enhance enzyme production and regulation in these hosts, and 3) discover novel pathways and organisms through bioprospecting to expand the range of chemicals to be synthesized and traits to be selected from. (aiche.org)
A complex interaction between the cleavage and polyadenylation molecular complex and cis -elements determine the polyadenylation site, which may result in the choice of non-canonical sites, resulting in alternative polyadenylation events, involved in the regulation of more than 80% of the genes expressed in plants. (frontiersin.org)
The present study explored whether SGK1 is transcriptionally regulated by angiotensin II and participates in the angiotensin II-dependent regulation of CTGF expression. (amrita.edu)
The synonymous codons were assumed to be neutral and to have no effect on the transgenic proteins, and presumed to be safe [2] ( Bt Toxins in Genetically Modified Crops: Regulation by Deceit , SiS 22). (i-sis.org.uk)
Macrophage inflammatory protein-2: chromosomal regulation in rat intestinal epithelial cells. (qmul.ac.uk)
J Virol, 79 2005), a DNA sequence that is responsible for the regulation of estrogen responsive genes in animals. (usda.gov)
Cotransfection assays, using expression vectors for thyroid receptor (TR) or chicken ovalbumin upstream promoter transcription factor 1 (COUP-TF1) and the CAV promoter driving expression of EGFP, were used to test for transcription regulation by these members of the nuclear receptor family that also recognize a consensus ERE. (usda.gov)
These strategies involve rearrangements at the molecular level starting from transcription, regulation of mRNA processing, translation, and protein modification or its turnover. (intechopen.com)
We have investigated potential mechanisms of regulation of rotavirus gene expression by functional evaluation of two differentially expressed viral mRNAs. (microbiologyresearch.org)
The study indicates that inducing the expression of LAV-BPIFB4 in aged mice improved cardiac function and regulation of blood flow to the heart. (medicalnewstoday.com)

The aim of this study was to investigate the expression and silencing of the S100A8 gene, which encodes the S100 calcium-binding protein A8 (S100A8), and apoptosis and phosphorylation of protein kinase B (Akt) in tissue samples of endometrial carcinoma and HEC-1A endometrial adenocarcinoma cells in vitro . (medscimonit.com)
This gene encodes a ribosomal protein that is a component of the 60S ribosome subunit. (nih.gov)
It was found that the C . crescentus genome encodes for more two-component signal transduction proteins that assist in cell cycle progression than any other bacterial genome sequenced so far. (kenyon.edu)
This was studied by using the CtrA gene, which encodes for an important cell cycle regulatory protein. (kenyon.edu)
In the process, they uncovered a relationship between body mass index and higher-than-usual expression of the gene ARVCF, which encodes a protein from the catenin family that is known for its role in adherent junction protein-protein interactions. (genomeweb.com)

The signaling pathway enhances access to the promoters of genes encoding inflammatory mediators, thus increasing their expression. (medindia.net)
In a Novartis-sponsored study in the New England Journal of Medicine, researchers found that a CRISPR-Cas9-based treatment targeting promoters of genes encoding fetal hemoglobin could reduce disease symptoms. (genomeweb.com)

In this study, we performed a new bioinformatics analysis on microarray-based gene expression data obtained from the public domain to explore genes that were differentially induced by drugs between the group of capreomycin and PA-824 and the group of mainly the first-line TB drugs. (hindawi.com)
Thus, stress response studies during these growth stages reveal novel differentially regulated genes or proteins with important functions in plant stress adaptation. (intechopen.com)

There are multiple pseudogenes of this gene dispersed throughout the genome. (nih.gov)
DNA snippets scattered across the genome harbor the code that directs the script of a cell's life, successively switching genes on and off. (mpg.de)
According to the prevailing theory, transcription factor proteins recognize "codewords" in enhancers that are specific for a certain cell type, allowing the genome to tell a cell's story by jumping to the right chapters. (mpg.de)
Some cross-references in the storybook of the genome might still hide in other regulatory sequences, like promoter regions that are in close proximity to the gene itself. (mpg.de)
In terms of the distribution of identified genes in a specific category relative to the whole genome, only the categories of PE/PPE and conserved hypotheticals have statistical significance. (hindawi.com)
At least 50,000 lncRNAs are expressed from intergenic regions of the human genome, more than twice the number of protein-coding genes [ 5 ]. (biomedcentral.com)
The genome of Caulobacter crescentus is structured in a circular chromosome and is comprised of 4,016,942 bp encoding for 3,767 genes. (kenyon.edu)
According to The Cancer Genome Atlas (TCGA), the speckle-type POZ protein (SPOP) mutant form is one of the significant core subtypes of PCa. (bvsalud.org)

AREB/ABFs are positively regulated by SnRK2s, controlling the activity of target proteins via phosphorylation. (wikipedia.org)
ACVR2 transmits the growth effects of activin via phosphorylation of SMAD proteins to affect gene transcription. (nih.gov)

Using the bioinformatics tool MAGICTRICKS and reversible pharmacological inhibition, we found that primed genes required the transcriptional activities of NF-κB. (frontiersin.org)
This control consists mainly of transcriptional factors, such as dehydration-responsive element-binding protein (DREB), abscisic acid (ABA)-responsive element-binding factor (AREB), and NAM (no apical meristem). (wikipedia.org)
The native form of AREB1 cannot target drought stress genes like RD29B in Arabidopsis, so modification is necessary for transcriptional activation. (wikipedia.org)
The availability of multiple PAS in the same 3' regulatory region enables the inclusion or exclusion of 3' untranslated region (3'UTR) sequences, resulting in transcripts that may differ in particularities involving post-transcriptional processes such as stability, transport and translation ( Mayr, 2016 ), and even protein localization ( Berkovits and Mayr, 2015 ). (frontiersin.org)
The related protein in chicken can bind to c-Jun and can repress c-Jun-mediated transcriptional activation. (nih.gov)
A ubiquitously expressed sequence-specific transcriptional repressor that is normally the target of signaling by NOTCH PROTEINS. (harvard.edu)
These two classes of 5′ capped and polyadenylated RNA transcripts are indistinguishable with regard to their length, number of exons or transcriptional orientation relative to their closest neighboring gene. (biomedcentral.com)
These data define a regulatory mechanism for the difference in amounts of VP6 and NSP1 and provide evidence for post-transcriptional control of rotavirus gene expression mediated by the translational efficiency of individual viral mRNAs. (microbiologyresearch.org)
Alternatively nuclear receptor coactivators can function as adaptor proteins that bring nuclear receptors into close proximity with transcriptional complexes. (bvsalud.org)
Transcriptional gene silencing of this regulatory element repressed TERT expression in an allele-specific manner. (lu.se)
Thus, enhanced extracellular matrix or beta-glucan synthesis during biofilm growth might prevent antifungals, such as azoles and polyenes, from reaching biofilm cells, thus limiting their toxicity to these cells and the associated transcriptional responses. (who.int)

All recognized mutations for AD are associated with increased deposition of amyloid-beta (Abeta), a peptide fragment comprising 39-43 amino acids that derive from the catabolism of the amyloid precursor protein (APP) molecule. (medscape.com)

Several two-component signal transduction proteins are involved in the cell cycle progression by accumulating at one or both poles "in a spatial and temporal pattern that is reproduced during each cycle" (Jacobs-Wagner 2003). (kenyon.edu)

Although a small number of 3' regulatory regions have been identified and validated so far, many studies have shown that plant 3' regulatory regions have a higher potential to regulate gene expression in plants compared to widely used 3' regulatory regions, such as NOS and OCS from Agrobacterium tumefaciens and 35S from cauliflower mosaic virus. (frontiersin.org)
He developed the concept that luminal factors regulate genes in the epithelium that signal to the mucosal immune system. (qmul.ac.uk)
The molecular steps in the replication pathway that regulate the levels of viral gene expression are not well defined. (microbiologyresearch.org)
The γ -secretase then liberates A β , as well as an APP cytoplasmic fragment, which may move to the nucleus and regulate gene expression. (csbsju.edu)

This system regulates the expression of the genes involved in the degradation of toluene, benzene, and ethylbenzene through the toluene dioxygenase pathway. (nih.gov)
The complex cleaves Notch (left) to generate a fragment (NICD) that moves to the nucleus and regulates the expression of genes involved in brain development and adult neuronal plasticity. (csbsju.edu)

IL-17 induced NOTCH1 activation in oligodendrocyte progenitor cells enhances proliferation and inflammatory gene expression. (harvard.edu)
Across cell types, these genes affect neuronal morphogenesis and neuronal communication, while neural progenitor cells show strong enrichment for development of the limbic system. (springer.com)

Overexpression of these genes enhance the tolerance of drought, high salinity, and low temperature in transgenic lines from Arabidopsis, rice, and tobacco. (wikipedia.org)
However, the toxicity of the transgenic proteins in animals and humans was not studied. (i-sis.org.uk)
Future research programs can be focused on the development of transgenic plants with enhanced stress tolerance in field conditions based upon the outcome of genomic approaches and knowing the mystery of nucleotides sequences hidden in cells. (intechopen.com)
To facilitate the study of these neurons, transgenic mice were generated that express enhanced green fluorescent protein (EGFP) in subpopulations of GABAergic neurons. (jneurosci.org)
Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)

Expression and estrogen sensitivity of target genes and nuclear receptor coregulators were altered at mRNA and protein levels in adult uterus, prostate and brain. (researchgate.net)

We, therefore, tested the hypothesis that hypoxia would enhance or "prime" microglial pro-inflammatory gene expression in response to a later inflammatory challenge without programmatically increasing basal levels of pro-inflammatory cytokine expression. (frontiersin.org)
We used RNA sequencing coupled with chromatin immunoprecipitation sequencing to analyze primed microglial inflammatory gene expression and modifications to histone H3 lysine 4 trimethylation (H3K4me3) at the promoters of primed genes. (frontiersin.org)

We compared the survivability of E. coli XL1-Blue cells transformed with a recombinant plasmid containing a glutathione S-transferase (GST)-Oshsp16.9 fusion protein (pGST-FL cells) with the control E. coli cells transformed with the pGEX-2T vector (pGST cells) under heat-shock (HS) after isopropyl β-D-thiogalactopyranoside induction. (ncu.edu.tw)
Due to the complex cellular composition of specific brain areas, neuronal type-specific recombinant gene expression is required for many potential applications of neuronal gene transfer. (harvard.edu)
Immunofluorescent costaining was performed to detect both recombinant gene products and nigrostriatal neurons. (harvard.edu)
Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris. (lu.se)
Proteomic analysis identifies allele-preferred binding of Zinc finger protein 148 (ZNF148) to rs36115365- C, further supported by binding of purified recombinant ZNF148. (lu.se)

Until the current decade, synonymous mutations were assumed to be neutral, with no effect on the protein or any other functions of the organism. (i-sis.org.uk)
Synonymous mutations may lead to changes in protein folding related to translation pausing, RNA splicing, and alterations in enzyme specificity [1]. (i-sis.org.uk)
Synonymous mutations may lead to ribosome stalling, thereby changing protein folding pathways affecting enzyme activity or antigenicity [5]. (i-sis.org.uk)
Synonymous mutations in the HIV gene Rev enhance HIV-1 replication, providing resistance to the drug enfuvirtide [6]. (i-sis.org.uk)
It is now clear that synonymous mutations are not neutral but instead frequently adversely affect the proteins encoded. (i-sis.org.uk)
It is reasonable to assume that the synonymous mutations may have created proteins with adverse phenotypes and contributed to the deterioration in public health, and this must now be thoroughly investigated together with mandatory labelling of GM products. (i-sis.org.uk)
Spastic paraplegia type 31 is caused by mutations in the REEP1 gene. (medlineplus.gov)
REEP1 gene mutations that cause spastic paraplegia type 31 result in a short, nonfunctional protein that is usually broken down quickly. (medlineplus.gov)
It is unclear how REEP1 gene mutations lead to the signs and symptoms of spastic paraplegia type 31. (medlineplus.gov)
Mutations in presenilin-1 that cause early-onset Alzheimer's disease enhance γ -secretase activity and A β production, and also perturb the ER calcium balance. (csbsju.edu)
FMF mutations are gain-of-function, that is, they confer new or enhanced activity on a protein, with a gene dosage effect (ie, more copies of the abnormal gene convey a greater effect). (msdmanuals.com)
Gene mutations result in altered pyrin molecules that do not inhibit inflammasome activation and thus cannot suppress minor, unknown triggers to inflammation that are normally checked by intact pyrin. (msdmanuals.com)

A gene encoding the rice 16.9-kDa class I low-molecular-mass (LMM) heat- shock protein (HSP), Oshsp16.9, was introduced into Escherichia coli using the pGEX-2T expression vector to analyze the possible function of this LMM HSP under heat stress. (ncu.edu.tw)
These fascinating mutants are a hands-on way to analyze genes. (kenyon.edu)
Autosomal Recessive Genetic disorders determined by a single gene (Mendelian disorders) are easiest to analyze and the most well understood. (msdmanuals.com)

Novagen Rosetta strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. (sigmaaldrich.com)
In addition, after transcription, a wide array of RNA-binding proteins interacts with cis -acting elements located mainly in the 3' untranslated region, determining the fate of mRNAs in eukaryotic cells. (frontiersin.org)

Overexpression of DREB2Aca enhances drought and heat stress tolerance levels in Arabidopsis. (wikipedia.org)
In contrast, overexpression of zili promoted expression of dorsal marker genes, while it inhibited ventral marker genes. (bioone.org)

This approach alters the grafts' response in the injury environment and confers enhanced neuronal differentiation capacity, survival, and integration, as well as reduced glial scar formation to provide a more effective stem cell therapy for severe traumatic SCI. (genengnews.com)
Consistent with other studies, genes identified by disrupted protein interactions are expressed early in development and in excitatory and inhibitory neuronal lineages. (springer.com)
Using inferred gene co-expression for three neuronal cell types-excitatory, inhibitory, and neural progenitor-we implicate several hundred genes in risk (FDR \(\le \hspace{0.17em}\) 0.05), ~ 60% novel, with characteristics of genuine ASD genes. (springer.com)
Background: Direct gene transfer into neurons has potential for developing gene therapy treatments for specific neurological conditions, and for elucidating neuronal physiology. (harvard.edu)
A second approach to achieve neuronal type-specific expression is to use a cell type-specific promoter, and we have used the tyrosine hydroxylase (TH) promoter to restrict expression to catecholaminergic neurons or a modified neurofilament heavy gene promoter to restrict expression to neurons, and both of these promoters support long-term expression from HSV-1 vectors. (harvard.edu)
To both improve nigrostriatal-neuron specific expression, and to establish that targeted gene transfer can be followed by long-term expression, we performed targeted gene transfer with vectors that support long-term, neuronal-specific expression. (harvard.edu)
The combination of targeted gene transfer with neuronal-specific promoters improved nigrostriatal neuron-specific expression (83 to 93%) compared to either approach alone, and supported long-term (1 month) expression at levels similar to those observed using untargeted gene transfer. (harvard.edu)
Conclusion: Targeted gene transfer can be used in combination with neuronal-specific promoters to achieve a high level of nigrostriatal neuron-specific expression. (harvard.edu)
GABAergic neurons, the chief inhibitory neurons of the mammalian brain, are comprised of diverse neuronal subtypes distinguishable on the bases of location in given brain regions, microanatomical features, including dendritic arborizations and axonal projections, expression of specific macromolecules, and neurophysiological properties. (jneurosci.org)

DREB2 also induces the expression of heat-related genes, such as heat shock protein. (wikipedia.org)
heat shock protein 90 (HSP90) is one of the most plenty proteins in breast cells. (bvsalud.org)

Ribosomal protein L10 in mitochondria serves as a regulator for ROS level in pancreatic cancer cells. (nih.gov)
The function of the REEP1 protein in the mitochondria is unknown. (medlineplus.gov)
Given the central role that mitochondria play during hypoxia, we hypothesized that Txnip deletion would enhance ischemia-reperfusion damage. (jci.org)
Thus, although Txnip deletion suppresses mitochondrial function, protection from myocardial ischemia is enhanced as a result of a coordinated shift to enhanced anaerobic metabolism, which provides an energy source outside of mitochondria. (jci.org)

All current GM crops use protein coding genes from bacteria that have been altered by introducing synonymous codons, replacing plant-preferred codons for the bacterial codons in order to enhance the production of protein from the transgenes. (i-sis.org.uk)
In synthesizing transgenes for GM crops, say, a Bacillus thuringiensis (Bt) cry toxin gene, a table of plant preferred codons is used to substitute for the bacterial codons [3]. (i-sis.org.uk)
Essentially all commercial GM crops contain bacterial genes with codons altered to enhance the expression of transgenes in plants. (i-sis.org.uk)

Different correlations between the levels of elncRNAs, plncRNAs and expression of neighboring genes suggest that functional lncRNAs from the two classes may play contrasting roles in regulating the transcript abundance of local or distal loci. (biomedcentral.com)
The researchers reported on their developments in Advanced Science , in a paper titled, " Transplanting Human Neural Stem Cells with ≈50% Reduction of SOX9 Gene Dosage Promotes Tissue Repair and Functional Recovery from Severe Spinal Cord Injury ," in which they concluded, "Our findings represent a new paradigm in generating genetically modified hNSCs for the treatment of SCI. (genengnews.com)
We investigate the functional relevance of de novo missense variants, specifically whether they are likely to disrupt protein interactions, and nominate novel genes in risk for ASD through integrated genomic, transcriptomic, and proteomic analyses. (springer.com)
As a result, there is a reduction in functional REEP1 protein. (medlineplus.gov)
We found that functional inhibition, genetic ablation, and inactivation by post-translational modification of OGG1 significantly augment IFN-λ expression in epithelial cells infected by human respiratory syncytial virus (RSV). (lu.se)
In a mouse model of bronchiolitis induced by RSV infection, functional ablation of OGG1 by a small molecule inhibitor (TH5487) enhances IFN-λ production, decreases immunopathology, neutrophilia, and confers antiviral protection. (lu.se)

Exocytosis of secretory or synaptic vesicles is executed by a mechanism including the SNARE (soluble N -ethylmaleimide-sensitive factor attachment protein receptor) proteins. (jneurosci.org)
This construct had increased expression in an estrogen receptor-enhanced cell line when treated with estrogen. (usda.gov)
Cotransfection assays were done, using expression vectors for thyroid receptor (TR) or chicken ovalbumin upstream promoter transcription factor 1 (COUP-TF1) to increase the levels of these hormone receptors in the cells containing the CAV promoter driving expression of EGFP. (usda.gov)
Expression of enhanced green fluorescent protein (EGFP) under control of the promoter-enhancer of chicken infectious anemia virus (CAV) is increased in an estrogen receptor-enhanced cell line when treated with estrogen. (usda.gov)
Abstract for "Fatty Acid-binding Protein 5 (FABP5) Regulates Cognitive Function Both by Decreasing Anandamide Levels and by Activating the Nuclear Receptor Peroxisome Proliferator-activated Receptor β/δ (PPARβ/δ) in the Brain" by Shuiliang Yu, Liraz Levi, Gemma Casadesus, George Kunos, and Noa Noy in Journal of Biological Chemistry . (neurosciencenews.com)
This gene provides instructions for making a protein called receptor expression-enhancing protein 1 (REEP1), which is found in neurons in the brain and spinal cord. (medlineplus.gov)
As a critical PCa driver and an SPOP-targeted protein, androgen receptor (AR) also plays a role in PCa initiation and progression. (bvsalud.org)

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. (sigmaaldrich.com)
His current laboratory in London examines how dietary factors act on genes in the epithelium both through promoter-based mechanisms and through changes in chromosomal structure. (qmul.ac.uk)
The chicken infectious anemia virus (CAV) gene promoter fused to the gene for enhanced green fluorescent protein (EGFP) was used to measure virus activity in response to estrogen. (usda.gov)
The virus promoter-enhancer DNA sequence was also found to bind some of the proteins that recongnize a consensus estrogen response element (ERE) (Miller et al. (usda.gov)
Coexpression with COUP-TF1 but not TR or a control RSV promoter vector was found to decrease expression of EGFP by 50-60% in fibrocytic and liver cell lines. (usda.gov)
Mutation of an E box-like sequence at the TSP restored expression of this longer promoter to the same level as the short promoter. (usda.gov)
This promoter-enhancer also binds unidentified proteins that recognize a consensus estrogen response element (ERE) (Miller et al. (usda.gov)
In an IHF-deficient background, expression from the tod promoter drops 8-fold. (nih.gov)
Results: Helper virus-free HSV-1 vector packaging was performed using either gC - GDNF or gC - BDNF and vectors that contain either the TH promoter or the modified neurofilament heavy gene promoter. (harvard.edu)

Thioredoxin-interacting protein (Txnip) is a regulator of metabolism and an inhibitor of the antioxidant thioredoxins, but little is known about its roles in the myocardium. (jci.org)
both methods indicated suppression of genes and proteins participating in mitochondrial metabolism. (jci.org)
Deletion of Txnip in the myocardium results in mitochondrial dysfunction with decreased expression of transcripts encoding mitochondrial metabolism. (jci.org)

Phosphatase and tensin homolog (PTEN) is well recognized as a tumor suppressor gene. (nature.com)

MMA-SS exposure also increased in vitro and in vivo protein expression of Nrf2 and HO-1 and in vitro gene expression of Hmox1. (cdc.gov)
Here, we show that p16 Ink4a deficiency enhances fasting-induced hepatic glucose production in vivo by increasing the expression of key gluconeogenic genes. (diabetesjournals.org)

This involves an initial cleavage of the amyloid precursor protein (APP) by an enzyme called BACE (or β -secretase). (csbsju.edu)

Noy and her Case Western Reserve School of Medicine and National Institute on Alcohol Abuse and Alcoholism colleagues particularly wanted to understand how this protein functioned in neurons. (neurosciencenews.com)
The researchers showed that human pluripotent stem cell (hPSC)-derived neural stem cells engineered with reduced expression of a gene called SOX9 differentiate preferentially into spinal motor neurons. (genengnews.com)
HSV-1 vector particles containing either gC - GDNF or gC - BDNF target gene transfer to nigrostriatal neurons, which contain specific receptors for GDNF or BDNF. (harvard.edu)

It is thought that this gene is upregulated in response to endoplasmic reticulum stress and that the glutathione depletion enhances apoptosis. (nih.gov)
The REEP1 protein plays a role in regulating the size of the endoplasmic reticulum and determining how many proteins it can process. (medlineplus.gov)

The 3' regulatory regions have a great diversity of cis -regulatory elements directly involved in polyadenylation, stability, transport and mRNA translation, essential to achieve the desired levels of gene expression. (frontiersin.org)
In this review, we discuss the role of 3' regulatory regions in gene expression, and the superior potential that plant 3' regulatory regions have compared to NOS, OCS and 35S 3' regulatory regions. (frontiersin.org)
The TodS and TodT proteins form a previously unrecognized and highly specific two-component regulatory system in which the TodS sensor protein contains two input domains, each of which are coupled to a histidine kinase domain. (nih.gov)
Throughout the cell cycle, the chromosome progressively goes from being fully methylated to hemimethylated during DNA replication - this results in differential binding of regulatory proteins to activate or repress transcription. (kenyon.edu)
Using array-based CNV profiles for more than 405,300 extensively phenotyped UK Biobank participants, the researchers studied traits or conditions associated with genes or regulatory sequences in the 22q11.2 region. (genomeweb.com)
Meanwhile, enhanced expression of another gene in the region - the potential regulatory gene DGCR6 - appeared to cause a dip in mean platelet volume. (genomeweb.com)
[ 15 ] The surface of the APC contains 2 peptide-binding proteins (ie, major histocompatability complex [MHC] classes I and II), which can stimulate cytotoxic T (T C ) cells, regulatory T (Treg) cells, and helper T (T H ) cells. (medscape.com)
Of these, rs36115365-C associated with increased pancreatic and testicular but decreased lung cancer and melanoma risk, and exhibited preferred protein-binding and enhanced regulatory activity. (lu.se)

Some of these include dehydration-responsive element-binding protein (DREB), ABA-responsive element-binding factor (AREB), no apical meristem (NAM), Arabidopsis transcription activation factor (ATAF), and cup-shaped cotyledon (CUC). (wikipedia.org)
Ectopic expression of CgNLP1 in Arabidopsis significantly enhanced the resistance to Botrytis cinerea and A. brassicicola . (biorxiv.org)

AREB/ABFs are ABA-responsive bZIP-type TFs which bind to ABA-responsive elements (ABREs) in stress-responsive promoters and activate gene expression. (wikipedia.org)
How do cells know when to activate a certain gene? (mpg.de)
In some cases, they are located far away from the gene they activate. (mpg.de)
The researchers developed a program that is able to identify DNA sequences that are recognized by the cell in order to activate genes in a tissue-specific way. (mpg.de)
Interferons (IFNs) are secreted cytokines with the ability to activate expression of IFN stimulated genes that increase resistance of cells to virus infections. (lu.se)
Dendritic cells display a large amount of MHC-peptide complexes at their surface and can increase the expression of costimulatory receptors and migrate to the lymph nodes, spleen, and other lymphoid tissues, where they activate specific T cells. (medscape.com)

Surprisingly, however, the majority of primed genes were not enriched for H3K4me3 acutely following hypoxia exposure. (frontiersin.org)

When the cell lysates from these two E. coli transformants were heated at 55°C, the amount of protein denatured in the pGST-FL cells was 50% less than that of the pGST cells. (ncu.edu.tw)
Similar results as pGST-FL cells were obtained in pGST-N78 cells (cells produced a fusion protein with only the N- terminal 78 aa in the Oshspl6.9 portion) but not in pGST-C108 cells (cells produced a fusion protein with C-terminal 108 aa in the Oshspl6.9 portion). (ncu.edu.tw)
The acquired thermotolerant pGST-FL cells synthesized three types of HSPs, including the 76-, 73-, and 64-kDa proteins according to their abundance at a lethal temperature of 47.5°C. This finding indicates that a plant class I LMM HSP, when effectively expressed in transformed prokaryotic cells that do not normally synthesize this class of LMM HSPs, may directly or indirectly increase thermotolerance. (ncu.edu.tw)
A stable S100A8 gene knockdown cell line was constructed using lentiviral packing short hairpin RNA (shRNA) transfected into HEC-1A cells. (medscimonit.com)
The effects of expression of the S100A8 gene by endometrial cancer cells was investigated by the MTT assay, cell cycle and apoptotic assays, qRT-PCR, and Western blotting. (medscimonit.com)
HCT116+chr2 cells and HEC59+chr2 cells, but not ACVR2-mutant HCT116 or HEC59 cells, acquired wild-type ACVR2 as well as expression of ACVR2 wild-type messenger RNA. (nih.gov)
Lung injury (LDH) and inflammation (neutrophil influx) were increased with ITI MMA-SS treatment compared to saline control and GMA-MS. RAW264.7 cells treated with MMA-SS caused increases in ROS, lipid aldehyde (4-hydroxynonenal) production, and COX-2 protein/gene expression. (cdc.gov)
Second, FABP5 regulates gene expression, a process that essentially gives cells their marching orders on structure, appearance and function. (neurosciencenews.com)
The genes that are induced in this case tell the cells to take steps that promote learning and memory. (neurosciencenews.com)
This study demonstrates that it is also possible to make human cardiac cells younger and older mice hearts by transferring a gene expressed by centenarians. (medicalnewstoday.com)
We also demonstrate that the benefit is related to the ability of the gene to reprogram cardiac cells to become more resistant to stress and build up the machinery ( ribosomes ) that make proteins. (medicalnewstoday.com)
When a microRNA is present in the cell, it binds to the target sites and downregulates GFP expression, while in cells that do not express the microRNA GFP, it is expressed. (lu.se)

Expression host strains for pETBlue and pTriEx vectors are lDE3 lysogens that also carry the compatible pLacI plasmid, encoding the lac repressor. (sigmaaldrich.com)
Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. (sigmaaldrich.com)
Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)

Therefore, specific inhibitors targeting proteins of the PI3K/AKT pathway are now being evaluated in clinical trials as a new approach against breast cancer 14 . (nature.com)
A new research led by Tokyo Medical and Dental University (TMDU) in chemically induced mouse model of inflammatory bowel disease (IBD) reveals the role of protein signaling pathway in the generation of macrophages that may induce inflammation in the colon. (medindia.net)
A team of Japanese scientists led by Tokyo Medical and Dental University (TMDU) used a chemically induced mouse model of IBD to show that a protein signaling pathway is crucial to the generation of macrophages that cause colonic inflammation. (medindia.net)

9-cis-retinoic acid enhances fatty acid-induced expression of the liver fatty acid-binding protein gene. (unil.ch)
The findings regarding the potential virtues of fatty acid binding protein 5 (FABP5) - usually associated with cancer and psoriasis - appear in the May 2 edition of The Journal of Biological Chemistry . (neurosciencenews.com)

Inflammatory monocytes and macrophages in the colons of IBD mice showed increased expression of proteins that promote inflammatory responses compared with other cell populations. (medindia.net)
Mice lacking Stat1 expression differentiated fewer inflammatory macrophages from their monocyte lineage, and additionally developed less severe inflammation of their colons than control mice," study first author Yusuke Nakanishi says. (medindia.net)
In the new study, inducing the expression of LAV-BPIFB4 in aged mice led to improvements in cardiac function that, translated to the human context, would be equivalent to a reduction in the heart's biological age by 10 years. (medicalnewstoday.com)
Unexpectedly, 8-oxoguanine DNA glycosylase1 (Ogg1) knockout mice show enhanced stimuli-driven IFN expression that confers increased resistance to viral and bacterial infections and allergen challenges. (lu.se)
Aquaporin-9 Protein Is the Primary Route of Hepatocyte Glycerol Uptake for Glycerol Gluconeogenesis in Mice. (lu.se)

TodS has basal autophosphorylation activity in vitro, which is enhanced by the presence of effectors. (nih.gov)
These in vitro, and their expression in these human tumour virus. (who.int)

The mRNAs are extruded into the cytoplasm where they serve as mRNA for protein synthesis and as templates for packaging and replication into dsRNA. (microbiologyresearch.org)

They also expressed more Stat1, which is a transcription factor that upregulates gene expression in response to stimulation by IFNγ, a signaling molecule released in the presence of pathogens. (medindia.net)
They attract transcription factor proteins that start the expression of genes, thereby "enhancing" their activity. (mpg.de)
Transcription factor is a protein the controls the flow of genetic information). (neurosciencenews.com)
FABP5 improves learning and memory both because it delivers endocannabinoids to cellular machinery that breaks them down and because it shuttles compounds to a transcription factor that increases the expression of cognition-associated genes," Noy said. (neurosciencenews.com)
Then, that degraded product is picked up by the same protein (FABP5) and shuttled to the cell nucleus - specifically, to a transcription factor within it (station 2). (neurosciencenews.com)
Binding of the degraded product activates the transcription factor and allows it to induce expression of multiple genes. (neurosciencenews.com)

As plant genetic engineering has "advanced" the crucial active domains of toxins, and enzyme are "improved" to such an extent that the gene for the original source protein is hardly recognizable. (i-sis.org.uk)
Nigrostriatal neuron-specific expression may be useful for specific gene therapy approaches to Parkinson's disease or for genetic analyses of nigrostriatal neuron physiology. (harvard.edu)

Flow cytometric estimation of the apoptotic marker CD95 in peripheral neutrophils, lymphocytes and monocytes was done for 18 infants with non-oedematous protein energy malnutrition (PEM) and 12 oedematous ones, on hospital admission and after supervised nutritional rehabilitation, and compared with 12 matched controls. (who.int)
The MEFV gene normally codes a protein named pyrin, which is expressed in circulating neutrophils. (msdmanuals.com)

Researchers now tested how much information can be extracted from sequence data to predict which gene is active in which tissue. (mpg.de)
Change in base sequence that does not alter amino acid sequence of proteins encoded nevertheless may result in alterations of the protein that make it unsafe. (i-sis.org.uk)
Immunoglobulin J Recombination Signal Sequence-Binding Protein" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
This graph shows the total number of publications written about "Immunoglobulin J Recombination Signal Sequence-Binding Protein" by people in Harvard Catalyst Profiles by year, and whether "Immunoglobulin J Recombination Signal Sequence-Binding Protein" was a major or minor topic of these publication. (harvard.edu)
Below are the most recent publications written about "Immunoglobulin J Recombination Signal Sequence-Binding Protein" by people in Profiles. (harvard.edu)
Electromobility shift assays were done to find if proteins from the nucleus bind to the DNA sequence at the TSP. (usda.gov)
Assays showed that multiple proteins can bind to this sequence and that one of these is the transcription regulator delta-EF1 (dEF1). (usda.gov)
Nucleotide sequence and expression in Escherichia coli of the gene encoding the nonstructural protein NCVP2 of bovine rotavirus. (microbiologyresearch.org)
Cloning and nucleotide sequence of the simian rotavirus gene 6 that codes for the major inner capsid protein. (microbiologyresearch.org)

CRISPR-based tools will be created for gene editing and chromosomal integration of pathways. (aiche.org)

In this study, zinc and copper oxide nanoparticles (NPs) were synthesized using hemp (Cannabis sativa L.) leaves (ZnONP-HL and CuONP-HL), and their antifungal potential was assessed against Fusarium virguliforme in soybean (Glycine max L.). Hemp was selected because it is known to contain large quantities of secondary metabolites that can potentially enhance the reactivity of NPs through surface property modification. (cdc.gov)
A 300% increase in the expression of soybean pathogenesis related GmPR genes encoding antifungal and defense proteins confirmed that the biosynthesized NPs enhanced disease resistance against the fungal phytopathogen. (cdc.gov)

Changes in morphology were imaged, and changes in gene expression of CEC typical genes such as zonula occludens (ZO-1), sodium/potassium (Na/K)-ATPase, paired-like homeodomain 2 (PITX2), and collagen 8 (COL-8) were measured with real-time polymerase chain reaction. (lww.com)
Interestingly, FLU-exposed biofilms showed no significant changes in gene expression over the course of the experiment. (who.int)

The CDKN2A/B locus contains genes encoding cell cycle inhibitors, including p16 Ink4a , which have not yet been implicated in the control of hepatic glucose homeostasis. (diabetesjournals.org)

PTEN was validated by dual luciferase assay as a target gene of miR-183. (nature.com)
Polyadenylation is essential for the stability of the transcript, preventing the mRNA from being the target of posttranscriptional gene silencing (PTGS) via RNA-dependent RNA polymerase 6 (RDR6) in plants ( Luo and Chen, 2007 ). (frontiersin.org)
One approach is to target gene transfer to a specific type of neuron. (harvard.edu)

Gene expression changes and aging biomarkers in the women's blood samples showed significant improvement for all groups at the conclusion of the retreat, indicating benefits from merely being on vacation. (worldhealth.net)
Background: Breast cancer is a heterogeneous disease and differences in the expression levels of the ER, PR, and HER2 the triplet of established biomarkers used for clinical decision-making have been reported among breast cancer patients. (bvsalud.org)

Overall, our data show that FABP5 enhances cognitive function and that FABP5 deficiency impairs learning and memory functions in the brain hippocampus region," said senior author Noa Noy, PhD, a professor of pharmacology at the School of Medicine. (neurosciencenews.com)

Studies have shown that the longevity-associated variant (LAV) of the BPIFB4 gene is associated with a longer lifespan in humans and has protective effects in rodent models of cardiovascular disease. (medicalnewstoday.com)

It has been proven that the miR-183 expression is up-regulated in tumor tissues and patient serum in BC, which indicates its possibly an oncogenic role 15 , 16 . (nature.com)

The protein encoded by this gene is a gamma-glutamyl cyclotransferase that catalyzes the conversion of glutathione to 5-oxoproline and cysteinylglycine. (nih.gov)

The statistical classifiers can also identify DNA subsequences that might underlie cell type-specific gene activation. (mpg.de)

Using the newly released and larger ASC dataset, we confirm these observations and take them in several new directions: (1) By defining a set of genes encoding these disrupted protein interactors in ASD subjects and another for their siblings, we evaluate their expression patterns in developing brain from fetal to early postnatal development and within general cell types of brain tissue. (springer.com)

Utilizing our previous interactome perturbation predictor, we identify a set of missense variants that are likely disruptive to protein-protein interactions. (springer.com)
For genes encoding the disrupted interactions, we evaluate their expression patterns across developing brains and within specific cell types, using both bulk and inferred cell-type-specific brain transcriptomes. (springer.com)
Finally, we integrate protein interactions and cell-type-specific co-expression networks together with published association data to implicate novel genes in ASD risk in a cell-type-specific manner. (springer.com)
Extending earlier work, we show that de novo missense variants that disrupt protein interactions are enriched in individuals with ASD, often affecting hub proteins and disrupting hub interactions. (springer.com)
Disrupted protein interactions identify gene sets involved in risk for ASD. (springer.com)

Anatomy20

Organisms24

Diseases9

Chemicals and Drugs85

Analytical, Diagnostic and Therapeutic Techniques and Equipment38

Psychiatry and Psychology1

Phenomena and Processes79

Disciplines and Occupations2

Technology, Industry, Agriculture2

Information Science4

Regulation10

Encodes5

Promoters of genes2

Differentially2

Genome8

Phosphorylation2

Transcriptional11

Amino Acids1

Signal Transduction1

Regulate4

Regulates the expression2

Progenitor cells2

Transgenic5

MRNA and protein1

Inflammatory gene expression2

Recombinant5

Mutations12

Analyze3

Eukaryotic2

Overexpression2

Neuronal9

Heat- shock2

Mitochondria4

Codons3

Functional6

Receptor7

Promoter9

Metabolism3

Homolog1

Vivo2

Amyloid precurs1

Neurons3

Endoplasmic reticulum2

Regulatory8

Arabidopsis2

Activate6

H3K4me31

Cells12

Vectors3

Pathway3

Fatty2

Mice5

Vitro2

Synthesis1

Transcription factor6

Genetic2

Neutrophils2

Sequence9

Pathways1

Soybean2

Changes in gene expression2

Inhibitors1

Target3

Biomarkers2

Pharmacology1

Humans1

Oncogenic1

Glutathione1

Activation1

Fetal1

Interactions5