Protein gene recombinant proteins. Medical search. Frequent questions

Neither did antibody to the recombinant 37,000 dalton protein react with any treponemal proteins purified by fibronectin affinity chromatography. (bmj.com)
Finally, only sera of patients with primary syphilis possessed appreciable concentrations of antibody to recombinant P2 protein. (bmj.com)
If you can't find the antibody you need, please check CUSABIO protein custom service . (cusabio.com)
We took advantage of the antibody resources publicly available from the Human Protein Atlas project covering more than 80% of all human protein-coding genes. (nih.gov)

The recombinant protein in Escherichia coli lysates was labelled P2 (P2 star) to differentiate it from the comigrating adhesin protein called P2. (bmj.com)
Recent studies have shown that it may be possible to produce recombinant lysosomal proteins with microorganisms such as Escherichia coli and Saccharomyces cerevisiae. (wikipedia.org)
An S-peptide Fucus MT fusion protein expressed in Escherichia coli exhibited a relative molecular mass of ∼ 14 kDa. (portlandpress.com)

Compared with traditional protein expression systems, many processes are omitted in vitro E.coli, such as the transformation of plasmids, cell culture, collection, crushing and centrifugation, etc., which greatly improves working efficiency. (cusabio.com)
The new extracted gene was sequenced and cloned in E. coli, followed by protein purification using a C-terminal His-tag. (bvsalud.org)
In addition, salt resistance experiments showed that the survival of recombinant E. coli+DnaJ was 2.1 times more than control cells in 0.5 M NaCl. (bvsalud.org)
Furthermore, the number of recombinant E. coli BL21+DnaJ colonies was 7.7 times that of the control colonies in pH 8.5. (bvsalud.org)
Recombinant Human JNK2 protein(1-424 aa), fused to GST tag, was expressed in E. coli. (creativebiomart.net)
Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. (antikoerper-online.de)
RHOQ Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 225 amino acids (1-202 a.a) and having a molecular mass of 24.7kDa. (prospecbio.com)
SESN2 / HI95 Protein LS-G25128 is a Recombinant Mouse SESN2 / HI95 produced in E. coli aa 41-480 with His-S tag(s). (lsbio.com)
An E. coli strain that carries a plasmid encoding the cloned human histone H3.3 gene, H3F3A or H3F3B. (neb.com)
The drug substance anakinra is a recombinant protein (Interleukin-1 receptor antagonist) produced in E. coli bacteria containing an expression plasmid in which a synthetic gene coding for human IL-1Ra has been inserted.According to the Guideline on the Environmental Risk Assessment of Medicinal Products for Human Use (EMEA 2006), proteins are exempted from environmental risk assessment because they are unlikely to result in significant risk to the environment. (janusinfo.se)

Here, we describe a new method (immuno-SILAC) for the absolute quantification of proteins in complex samples based on polyclonal antibodies and stable isotope-labeled recombinant protein fragments to allow affinity enrichment prior to mass spectrometry analysis and accurate quantification. (nih.gov)
Epitope mapping revealed that a majority of the polyclonal antibodies recognized multiple linear epitopes, and based on these results, a semi-automated method was developed for peptide enrichment using polyclonal antibodies immobilized on protein A-coated magnetic beads. (nih.gov)
A protocol based on the simultaneous multiplex capture of more than 40 protein targets showed that approximately half of the antibodies enriched at least one functional peptide detected in the subsequent mass spectrometry analysis. (nih.gov)
Here, we show that we were able to use small amounts of antibodies (50 ng per target) in this manner for efficient multiplex analysis of quantitative levels of proteins in a human HeLa cell lysate. (nih.gov)
The results suggest that polyclonal antibodies generated via immunization of recombinant protein fragments could be used for the enrichment of target peptides to allow for rapid mass spectrometry analysis taking advantage of a substantial reduction in sample complexity. (nih.gov)
Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies. (antikoerper-online.de)
The recombinant proteins will be used as antigens to produce polyclonal antibodies. (usda.gov)
The antibodies will be used for western blots or immunostaining to detect the presence of the Hessian fly proteins in infested wheat tissues. (usda.gov)
Within the past 2 years, investigational peptide- and recombinant protein-based serologic assays that can more easily differentiate the antibodies to HTLV-I and HTLV-II have been developed (8,9). (cdc.gov)
however, rabbit antibodies raised against the recombinant proteins encoded by the gene of mouse bullous pemphigoid antigen 2 (BP180) are capable of inducing blisters in newborn mice in a complement-dependent manner. (medscape.com)
135 antibodies against 65 mainly immunoregulatory proteins, we of a multitude of different genes (8, 9). (lu.se)

Protein production Gene expression Protein purification Host cell protein Young CL, Britton ZT, Robinson AS (May 2012). (wikipedia.org)
We own protein expression technology based on a series of protein expression and purification technology, including protein expression technology shown by Baculovirus outer membrane proteins (OMP), and membrane protein expression technology, etc. (cusabio.com)
A His-tag will be added to each recombinant protein to facilitate protein purification. (usda.gov)
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization). (antibodies-online.com)
In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. (antibodies-online.com)
Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. (antibodies-online.com)
Purification and characterization of two protein kinases acting on the aquaporin SoPIP2;1. (lu.se)

Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. (rndsystems.com)
The human p28 gene encodes a 243 aa precursor that contains a 28 aa signal sequence and 215 aa mature region. (creativebiomart.net)
Although the protein sequence showed high identity with plant and invertebrate MTs, it contained a unique 'linker ' region (14 amino acid residues) between the two putative metal-binding domains which contained no cysteine residues. (portlandpress.com)

Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. (origene.com)
The encoded protein is secreted and binds hydroxyapatite with high affinity. (origene.com)
The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. (abcam.com)
Affinity tags can reduce merohedral twinning of membrane protein crystals. (lu.se)

If you are looking for recombinants with excellent quality (validated bio-activity, lower endotoxin level and high purity) to support your research, you are in the right place. (cusabio.com)
The purity of Human ACE2 (18-615) Recombinant Protein (hFc-Tag) was determined by densitometry after SDS-PAGE of 1 μg of protein, followed by staining with Coomassie Blue. (cellsignal.com)

Treponemal proteins ranging in molecular weight from 37,000 daltons to 120,000 daltons were identified by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and immunoblotting of phage lysate proteins. (bmj.com)
The mature protein has an approximate molecular weight of 24 kDa. (invivogen.com)
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. (novusbio.com)

The tyrosinase‐induced polymerization of peptides offers facile access to artificial mussel foot protein analogues. (wikipedia.org)
The use of amino acids, proteins and peptides is not considered to have any environmental impact. (janusinfo.se)
According to the European Medicines Agency guideline on environmental risk assessments for pharmaceuticals (EMA/CHMP/SWP/4447/00),vitamins, electrolytes, amino acids, peptides, proteins, carbohydrates, lipids proteins, vaccines and herbal medicinal products are exempted because they are unlikely to result in significant risk to the environment. (janusinfo.se)

In this study, we showed that the human RFC (hRFC) gene can serve as a novel selectable marker for the overproduction of recombinant proteins. (aspetjournals.org)
In many cases, recombinant human proteins have replaced the original animal-derived version used in medicine. (wikipedia.org)
The prefix "rh" for "recombinant human" appears less and less in the literature. (wikipedia.org)
The Recombinant Human Lysyl Oxidase Homolog 2 Protein is derived from NS0. (fishersci.com)
The Recombinant Human Lysyl Oxidase Homolog 2 Protein has been validated for the following applications: Enzyme Activity. (fishersci.com)
Binding response kinetics between Human ACE2 (18-615) Recombinant Protein (hFc-Tag) (immobilized) and SARS-CoV-2 Spike (trimeric) (16-1208) Recombinant Protein (8xHis-Tag) #65444 (in solution, at indicated concentrations). (cellsignal.com)
Recombinant human/mouse/rat BMP-2 (355-BM) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. (novusbio.com)
The human glucosamine-6-phosphate deaminase gene: cDNA cloning and expression, genomic organization and chromosomal localization. (nih.gov)
When Recombinant Human B7-H2 Fc Chimera Recombinant Human B7‑H2 Fc Chimera (Catalog # 165-B7 ) is immobilized at 0.1 µg/mL (100 µL/well), Biotinylated Recombinant Human ICOS Fc Chimera binds with an ED 50 of 1-6 ng/mL. (rndsystems.com)
Recombinant Human EBI3(Arg21-Lys229) and p28(Phe29-Pro243) fused with His tag at C-terminal was expressed in NS0. (creativebiomart.net)
The human EBI3 gene encodes a 229 amino acid (aa) precursor that contains a 20 aa signal peptide and 209 aa mature protein. (creativebiomart.net)
The following product was used in this experiment: Human MAP4K5 (KHS1), GST Tag Recombinant Protein from Thermo Fisher Scientific, catalog # PV3682. (thermofisher.com)
3M™ Zeta Plus™ Delipid Series Filter Media incorporates lipid-specific adsorbents for selective removal of lipids, surfactants and biological hydrophobes from human blood plasma, serum-based products, recombinant proteins and other biological fluids. (3m.com)
Human PRB1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade. (antibodies-online.com)
Here we describe a different method of transgene regulation by the use of the human glial ®brillary acidic protein (GFAP) promoter. (lu.se)
The use of viral vectors to deliver foreign genes to the brain is highly logical states in the human brain (Eng et al. (lu.se)

Several genes encoding putative Hessian fly effector family 31 members have been cloned previously. (usda.gov)
A gene was identified (1229 bp) with a putative open reading frame (204 bp) encoding a 67-amino-acid protein exhibiting several characteristic features of MT proteins, including 16 cysteine residues (24%) and only one aromatic residue. (portlandpress.com)

A dilution series of the recombinant Lucia luciferase protein can also be used to determine the linear range of the assay. (invivogen.com)

CD137 ligand (CD137L) is a type II membrane protein and part of the TNF superfamily. (fishersci.com)
Angiotensin-Converting Enzyme 2 (ACE-2) is an integral membrane protein and a zinc metalloprotease of the ACE family, the ACE family includes somatic and germinal ACE. (prosci-inc.com)
ST3GAL5 is a type II membrane protein which catalyzes the formation of GM3 with lactosylceramide as the substrate. (prospecbio.com)

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (rndsystems.com)

As a result, recombinant lysosomal proteins are usually produced in mammalian cells. (wikipedia.org)
A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. (antikoerper-online.de)
The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. (antikoerper-online.de)

Over more than 16 years, CUSABIO has established five systems from prokaryotic to eukaryotic to express the recombinant protein. (cusabio.com)
In addition, after transcription, a wide array of RNA-binding proteins interacts with cis -acting elements located mainly in the 3' untranslated region, determining the fate of mRNAs in eukaryotic cells. (frontiersin.org)
The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. (antikoerper-online.de)

Cloning structural genes for Treponema pallidum immunogens and characterisation of recombinant treponemal surface protein, P2 (P2 star). (bmj.com)

Moreover, PRIN2 and CSP41b form a distinct protein complex in vitro that binds DNA. (frontiersin.org)
In the active state it binds to a diversity of effector proteins to regulate cellular responses. (prospecbio.com)
Thus this paper describes, for the first time, an MT gene identified from macroalgae which is induced by copper exposure and whose encoded protein product binds cadmium and copper. (portlandpress.com)

Several transcript variants encoding different isoforms have been found for this gene. (origene.com)
The prin2.2 and csp41b-2 single mutants displayed pale phenotypes, abnormal chloroplasts with reduced transcript levels of photosynthesis genes and defects in embryo development. (frontiersin.org)
Two alternatively spliced transcript variants encoding the same protein have been described for MAP4K5. (thermofisher.com)
Polyadenylation is essential for the stability of the transcript, preventing the mRNA from being the target of posttranscriptional gene silencing (PTGS) via RNA-dependent RNA polymerase 6 (RDR6) in plants ( Luo and Chen, 2007 ). (frontiersin.org)
Through APA, a single gene containing multiple PAS can generate a considerable number of transcript isoforms, thereby producing a highly diversified transcriptome ( Tian and Manley, 2013 ). (frontiersin.org)
Transcript variants encoding various isoforms have been found for this gene. (prospecbio.com)

Here is the catalog of all recombinant proteins. (cusabio.com)
In addition to our diverse catalog and semi-custom recombinant protein products, we also offer comprehensive custom recombinant protein expression and production services to meet a variety of research needs. (avivasysbio.com)

Aviva Systems Biology has been providing high-quality protein products to the scientific community for over 10 years. (avivasysbio.com)
The course presents methods to express genes, which have products that are known, unknown, or not well characterised, and to analyse the gene products. (lu.se)

Spectroscopic results showed 56% higher carbonic anhydrase activity in the presence of the recombinant DnaJ homolog compared to its absence. (bvsalud.org)

Values on y-axis indicate binding response signals recorded for each protein in solution at the indicated concentration. (cellsignal.com)
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. (rndsystems.com)
Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20? (joplink.net)
The concentration of our recombinant proteins is measured using the absorbance at 280nm. (antibodies-online.com)
The concentration of the protein is calculated using its specific absorption coefficient. (antibodies-online.com)

Gene cloning and characterization of a novel recombinant 40-kDa heat shock protein from Mesobacillus persicus B48. (bvsalud.org)

The present study reports the recognition and characterization of the gene encoding the co-chaperone DnaJ in the halophilic strain Mesobacillus persicus B48. (bvsalud.org)

Native P2 protein was present on T pallidum surfaces as shown by radioimmunoprecipitation assays with extrinsically labelled organisms. (bmj.com)
Recombinant Lucia Luciferase Protein is a positive control for luciferase reporter assays. (invivogen.com)
This recombinant protein can be used for biological assays. (prosci-inc.com)

NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. (abcam.com)

2003). The therapeutic effects of different genes delivered by ment of the hGFAP promoter used here has been extensively studied and lentiviral vectors have been documented in a number of animal found to be up-regulated after several different stimuli both in vitro and models, both in rodents and primates, and examples include delivery in vivo in transgenic mice (Brenner et al. (lu.se)

The availability of multiple PAS in the same 3' regulatory region enables the inclusion or exclusion of 3' untranslated region (3'UTR) sequences, resulting in transcripts that may differ in particularities involving post-transcriptional processes such as stability, transport and translation ( Mayr, 2016 ), and even protein localization ( Berkovits and Mayr, 2015 ). (frontiersin.org)
However, the proteins needed for transcriptional control have been reported to be immunogenic and there is often a substantial leakage in the systems (Clackson, 2000). (lu.se)

This risk assessment was not dependent on the type of adju- decoding patterns of immunoregulatory serum proteins could re- vant therapy received by the patients. (lu.se)

Lyophilized from a 0.2 μm filtered solution in Glycine, Sucrose, Tween® 80 and Glutamic Acid with BSA as a carrier protein. (novusbio.com)
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. (rndsystems.com)
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). (prospecbio.com)

Moreover, a D614G mutation in the spike protein did not appear to affect SARS-CoV-2 cell tropism. (cdc.gov)

Toward this end, a hemagglutinin (HA) epitope tagged hRFC (hRFC-HA) was introduced into a bicistronic vector (pIRES2-EGFP), upstream of an enhanced green fluorescent protein (EGFP) reporter gene. (aspetjournals.org)

A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. (antikoerper-online.de)

Genetic Characterisation of Plasmodium falciparum Isolates with Deletion of the pfhrp2 and/or pfhrp3 Genes in Colombia: The Amazon Region, a Challenge for Malaria Diagnosis and Control. (cdc.gov)

The PEP enzyme recognizes the -10 and -35 cis -elements, similar to those found in bacterial promoters whereas the NEP enzyme recognizes the YRTA-motif, which can also be found upstream of several genes with PEP promoters indicating that these genes can be transcribed by both polymerases ( Pfannschmidt and Liere, 2005 ). (frontiersin.org)

Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. (nih.gov)

Cystatins are proteins that inhibit cysteine proteases. (wikipedia.org)
To investigate the possible existence of a metallothionein (MT) gene, the library was screened with degenerate probes designed using plant MT cysteine-rich motifs. (portlandpress.com)

Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris. (lu.se)

[ 1 ] As this gene is carried on the X chromosome, the disease usually manifests in males and is transmitted by females who carry the causative mutation on one of their X chromosomes. (medscape.com)

The His-tag may be also used to immobilize proteins to a solid support (such as resin) for identifying interacting targets in wheat. (usda.gov)
To identify interacting targets, Hessian fly-infested and non-infested wheat extracts will be incubated with immobilized family 31 proteins. (usda.gov)

The approach was further developed to also generate quantitative data via the addition of heavy isotope-labeled recombinant protein fragment standards prior to trypsin digestion. (nih.gov)

This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. (novusbio.com)

Recombinant proteins are useful tools for analyzing biological interactions. (cusabio.com)

The stability and function of the recombinant DnaJ protein under salt and pH stress conditions were evaluated. (bvsalud.org)
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. (joplink.net)
The 3' regulatory regions have a great diversity of cis -regulatory elements directly involved in polyadenylation, stability, transport and mRNA translation, essential to achieve the desired levels of gene expression. (frontiersin.org)

Lysosomal proteins: Lysosomal proteins are difficult to produce recombinantly due to the number and type of post-translational modifications that they have (e.g. glycosylation). (wikipedia.org)

Although a small number of 3' regulatory regions have been identified and validated so far, many studies have shown that plant 3' regulatory regions have a higher potential to regulate gene expression in plants compared to widely used 3' regulatory regions, such as NOS and OCS from Agrobacterium tumefaciens and 35S from cauliflower mosaic virus. (frontiersin.org)
The ability to regulate transgene expression will be crucial for development of gene therapy to the brain. (lu.se)
An aspect of gene delivery to the brain that is currently being regulate the endogenous GFAP expression in a lesioned brain. (lu.se)

A complex interaction between the cleavage and polyadenylation molecular complex and cis -elements determine the polyadenylation site, which may result in the choice of non-canonical sites, resulting in alternative polyadenylation events, involved in the regulation of more than 80% of the genes expressed in plants. (frontiersin.org)
These modifications have been shown to be important in gene regulation. (neb.com)

RHOQ protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 1mM DTT. (prospecbio.com)
ST3GAL5 protein solution (1mg/ml) containing In 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 0.4M Urea. (prospecbio.com)

Anatomy15

Organisms29

Diseases1

Chemicals and Drugs88

Analytical, Diagnostic and Therapeutic Techniques and Equipment46

Phenomena and Processes84

Disciplines and Occupations2

Technology, Industry, Agriculture6

Information Science4

Health Care5

Antibody4

Escherichia3

Coli10

Antibodies11

Purification7

Sequence3

Affinity4

Purity2

Molecular weight3

Peptides3

Human16

Putative2

Assay1

Membrane protein3

ELISA1

Mammalian3

Eukaryotic3

Immunogens1

Binds3

Transcript6

Catalog2

Products2

Homolog1

Concentration5

Cloning1

Characterization1

Assays3

Homo1

Different genes1

Transcriptional2

Serum proteins1

Carrier protein3

Spike protein1

Epitope1

Yeast1

Characterisation1

Promoters1

Carbonic anhydrase1

Cysteine2

Enhances1

Chromosome1

Targets2

Fragment1

Vitro1

Biological1

Stability3

Glycosylation1

Regulate3

Regulation2

20mM Tris-HCl buffer2