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  • cell
  • During retinal development, cell-cell and cell-ECM interactions are necessary for cell adhesion, migration, proliferation, and differentiation and are likely to be critical in establishing the highly organized architecture of the retina. (wiley.com)
  • How can the integrin adhesome get self-assembled locally, rapidly, and correctly as diverse cell-matrix adhesion sites? (elifesciences.org)
  • These can make the construction of cell-matrix adhesion sites quicker and more accurate by reducing the number of steps in the self-assembly process. (elifesciences.org)
  • VASP over-expressed in transiently transfected BHK21 cells was predominantly detected at stress fibres, at focal adhesions and in F-actin-containing cell surface protrusions, whereas truncated VASP lacking the C-terminal domain was no longer concentrated at focal adhesions. (nih.gov)
  • This supports an important role of zyxin in relaying signals from actin-rich cell adhesion sites to the nucleus. (biologists.org)
  • Cell adhesion and migration are integral components of many biological processes including normal development and recovery from injury and infection. (rupress.org)
  • VASP is a microfilament and focal adhesion associated protein which is also concentrated in highly dynamic regions of the cell cortex. (mysciencework.com)
  • Moreover, VASP and a novel protein were specifically extracted from total cell lysates by profilin affinity chromatography and subsequently eluted either with poly-L-proline or a peptide corresponding to a proline-rich VASP motif. (mysciencework.com)
  • However, the decreased survival and/or adhesion of MSCs under ischemic conditions limits the application of cell transplantation as a therapeutic modality. (yonsei.ac.kr)
  • Treatment of MSCs with PKC activator, phorbol 12-myristate 13-acetate (PMA), increased cell adhesion and spreading in a dose-dependent method and significantly decreased detachment. (yonsei.ac.kr)
  • Many especially, the adaptor proteins Crk is normally a principal docking partner and its association with phosphorylated Cas induce Rac-dependent cell migration and promotes growth cell intrusion . (elevateblogconference.com)
  • Cell adhesion is an important mechanism by which cells interact with the extracellular environment. (rupress.org)
  • FAK is situated at the signaling junctions where information comes from mainly four sources-ECM-integrin interaction, growth factor signaling, G-protein coupled receptor signaling and mechanical forces imposed on the cell [1, (scirp.org)
  • We have observed glucose-stimulated changes at the β-cell basal membrane similar to focal adhesion remodeling in cell migration. (diabetesjournals.org)
  • Cell-cell adhesion molecules are integral membrane proteins that are associated with peripheral membrane proteins to regulate and integrate cell-cell adhesion-related phenomena. (molvis.org)
  • In multicellular organisms, cell-cell adhesion is critical for development and morphogenesis. (molvis.org)
  • Here I seek to summarize the current state of knowledge about FAK and integrate relevant observations into known processes of cell adhesion, migration and growth regulation. (biologists.org)
  • Surprisingly, knockdown of NKCC1 in glioma cells resulted in the formation of significantly larger focal adhesions and cell traction forces that were approximately 40% lower than control cells. (nih.gov)
  • When esophageal adenocarcinoma cells were treated with TAE226, cell proliferation and migration were greatly inhibited with an apparent structural change of actin fiber and a loss of cell adhesion. (aacrjournals.org)
  • regulate
  • We hypothesize that CPNA-1 has at least 2 functions: (1) to anchor several proteins ( UNC-89 , UNC-96 , LIM-9 and SCPL-1 ) to the M-line in adult muscle, and (2) to regulate or co-operate with integrin at muscle focal adhesions during myofibril assembly. (wormbook.org)
  • structures
  • Given the large number of different proteins that can be used to build adhesion sites, and the large number of possible interactions between these proteins, a major question is-how are these structures self-assembled both rapidly and correctly? (elifesciences.org)
  • The Pat phenotype has been found in loss of function mutants of many components of the muscle focal adhesion structures (M-lines and dense bodies). (wormbook.org)
  • To further understand how CPNA-1 is localized to muscle focal adhesions, we used CPNA-1 to screen our 2-hybrid bookshelf of 30 known components of these structures. (wormbook.org)
  • 6-10 However, the mechanisms by which these structures and proteins detect physical forces and initiate biochemical signals are yet unclear. (ahajournals.org)
  • Lysates
  • Primary Antibodies Secondary Antibodies Proteins & Peptides Lysates. (acronymattic.com)
  • rho proteins in lysates from DNT-treated cells and untreated cells were radiolabeled by [32P]ADP-ribosylation with C3 exoenzyme and analyzed by SDS-polyacrylamide gel electrophoresis. (biologists.org)
  • receptors
  • Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2 , netrin receptors and LDL receptors. (rcsb.org)
  • binds
  • Geldanamycin (GA) is an ansamycin antibiotic that binds and inhibits the ATP dependent function of Hsp90 preventing the folding of client proteins, and many inhibitors used are based on the GA structure [ 8 - 12 ]. (impactjournals.com)
  • VASP
  • The hydrodynamic properties of purified human VASP together with the calculated molecular mass of cloned VASP suggest that the native protein is a homotetramer with an elongated structure. (nih.gov)
  • Finally, the subcellular distributions of VASP and profilin suggest that both proteins also interact within living cells. (mysciencework.com)
  • phosphotyrosine
  • Results Western blot analyses with three different antibodies against phosphotyrosine revealed that pp68 and pp125 were the two major tyrosine-phosphorylated proteins present in the normal carotid artery and the aorta. (bmj.com)
  • antibodies
  • Reprobing with various antibodies identified these proteins was paxi1lin and FAK. (bmj.com)
  • Affinity purified rabbit antibodies have been generated to CPNA-1 , and these antibodies react with a protein of ~130 kDa, the size expected for CPNA-1b. (wormbook.org)
  • inhibitor
  • When MSCs were treated with PKC inhibitor, that is, rottlerin, adhesion of MSCs was slightly diminished, and detachment was also decreased compared to the treatment with PMA. (yonsei.ac.kr)