• This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. (thermofisher.com)
  • This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. (thermofisher.com)
  • Our results demonstrate that cytoglobin expression is correlated with a more quiescent phenotype of stellate cells in culture and that cytoglobin is regulated by the extracellular matrix through integrin signalling dependent on activation of focal adhesion kinase. (biomedcentral.com)
  • An adhesive phenotype is developed with actin stress fibers and activation of focal adhesion kinase (FAK) and Rho GTPase. (princeton.edu)
  • Tenascin-C modulates matrix contraction via focal adhesion kinase- and Rho-mediated signaling pathways. (ox.ac.uk)
  • Using a synthetic model of the provisional matrix, we have found that tenascin-C regulates cell responses to a fibrin-FN matrix through modulation of focal adhesion kinase (FAK) and RhoA activation. (ox.ac.uk)
  • Podosomes are structurally divided into a core, which mainly contains proteins involved in actin polymerization (such as WASP, the Arp2/3 complex and cortactin ), and a surrounding ring populated by integrin receptors and adhesion proteins (for example, paxillin and focal adhesion kinase (FAK/Pyk2) ) [15] . (cellmigration.org)
  • RGD-independent cell adhesion to tTG-FN does not require transamidating activity, is mediated by the binding of tTG to cell-surface heparan sulfate chains, is dependent on the function of protein kinase Cα (PKCα) and leads to activation of the cell survival focal adhesion kinase (FAK). (ntu.ac.uk)
  • The morphological transition requires the RhoA-ROCK (Rho-associated coil-containing protein kinase) pathway and is prevented by RhoE. (rupress.org)
  • The interplay between mechanical strain and inflammation in pulmonary fibrosis remain incompletely understood.APPROACH: In this study, we used conditional KO mice to block mechanotransduction by knocking out FAK (Focal Adhesion Kinase) expression in fibroblasts, followed by induction of pulmonary fibrosis using bleomycin. (stanford.edu)
  • Noncanonical regulation of phosphatidylserine metabolism by a Sec14-like protein and a lipid kinase. (tamu.edu)
  • This is controlled during the cell adhesion procedure through molecules and signalling pathways following a biological cascade, including proliferation and Extracellular Matrix (ECM) production [ 9 ]. (alliedacademies.org)
  • The dynamic adhesion of cells to their surrounding extracellular matrix provides many of the environmental cues necessary for controlling cell migration and cell shape, survival, proliferation and differentiation. (upstate.edu)
  • We examined the correlation between nuclear deformation and cell functions such as cell proliferation, transfection and extracellular matrix protein type I collagen production. (cdc.gov)
  • The aim of the present study was to functionalize Ti scaffolds with genetically modified elastin-like recombinamers (ELRs), synthetic polymeric proteins containing the elastin epitopes responsible for their mechanical properties and for promoting mesenchymal stem cell (MSC) recruitment, proliferation, and differentiation to ultimately increase scaffold osseointegration. (ibecbarcelona.eu)
  • Cell adhesion, proliferation, and colonization were enhanced on those scaffolds functionalized with RGD-ELR, while differentiation was promoted on those with SNA15-ELR. (ibecbarcelona.eu)
  • The combination of both RGD and SNA15 into the same ELR stimulated cell adhesion, proliferation, and differentiation, although at lower levels than those for every single moiety. (ibecbarcelona.eu)
  • Further investigation on the amount and distribution of RGD and SNA15 moieties in ELRs could improve cell adhesion, proliferation, and differentiation compared to the present study. (ibecbarcelona.eu)
  • In addition, MAAs are also involved in the modulation of skin fibroblasts proliferation. (elsevierpure.com)
  • LPA acts as an autocrine/paracrine messenger through at least six G protein-coupled receptors (GPCRs), known as LPA 1-6 , to induce various cellular processes including wound healing, differentiation, proliferation, migration, and survival. (hindawi.com)
  • In the current study, we investigated the role of different extracellular matrix proteins in stellate cell proliferation, activation (alpha smooth muscle actin expression and retinoic acid uptake) and cytoglobin expression. (biomedcentral.com)
  • This has allowed us to reveal potential mechanisms controlling fibroblast fate during migration, proliferation, and differentiation following skin injury, and thereby reexamine the canonical phases of wound healing. (stanford.edu)
  • Paxillin, an integrin-assembly protein, has four major tyrosine phosphorylation sites, and the phosphorylation of Tyr31 and Tyr118 correlates with cell adhesion and migration. (silverchair.com)
  • Interaction with ECM is a major driver of HSC morphology [ 11 ], and signal transduction from integrin activation occurs at least partly through aggregation and subsequent activation of focal adhesion kinases (FAKs) by autophosphorylation of tyrosine 397 (Y397). (biomedcentral.com)
  • Fibroblasts adhere to fibronectin in this matrix via concomitant engagement of α5β1 integrin receptors and syndecan-4, a transmembrane proteoglycan. (princeton.edu)
  • tTG-FN supports RGD-independent cell adhesion of different cell types and the formation of distinctive RhoA-dependent focal adhesions following inhibition of integrin function by competitive RGD peptides and function blocking antiintegrin antibodies α5β1. (ntu.ac.uk)
  • We propose a novel RGD-independent cell adhesion mechanism that promotes cell survival when the anti-apoptotic role mediated by RGD-dependent integrin function is reduced as in tissue injury, which is consistent with the externalisation and binding of tTG to fibronectin following cell damage/stress. (ntu.ac.uk)
  • We are particularly interested in characterizing the function of the molecular scaffold/adapter proteins Paxillin and it's close relative Hic-5 during tumor cell migration and invasion. (upstate.edu)
  • Turner, C.E., (2000) Paxillin and focal adhesion signaling. (upstate.edu)
  • We found that Tyr31/118-phosphorylated paxillin competes with p190RhoGAP for binding to p120RasGAP, and provides evidence that p190RhoGAP freed from p120RasGAP efficiently suppresses RhoA activity during cell adhesion. (silverchair.com)
  • We conclude that Tyr31/118-phosphorylated paxillin serves as a template for the localized suppression of RhoA activity and is necessary for efficient membrane spreading and ruffling in adhesion and migration of NMuMG cells. (silverchair.com)
  • Regulation of cell migration by focal adhesion adapter proteins and their role in cancer cell metastasis. (upstate.edu)
  • We hypothesize that it is through these various interactions that the cell coordinates intracellular signaling and cytoskeletal reorganization to regulate cell adhesion, migration and tumor invasion. (upstate.edu)
  • We found that mutation of Tyr31/118 caused enhanced activation of RhoA and premature formation of stress fibers with substantial loss of efficient membrane spreading and ruffling in adhesion and migration of NMuMG cells. (silverchair.com)
  • This matrix serves as a scaffold for fibroblast migration into the wound where these cells deposit new matrix to replace lost or damaged tissue and eventually contract the matrix to bring the margins of the wound together. (ox.ac.uk)
  • Cell adhesion (the physical interaction of a cell with another cell or with the extracellular matrix (ECM)) is essential for cell migration and tissue integrity. (cellmigration.org)
  • Cell-cell adhesion maintains epithelial tissues, supports functional contacts between specialized cells, and can facilitate directed migration (for example, radial glia can guide neuronal progenitor cells to specific layers of the brain). (cellmigration.org)
  • Cell-matrix adhesion is the best-studied form of adhesion that mediates cell migration, and is the focus of this outline. (cellmigration.org)
  • Focal adhesions are large protein complexes organized at the basal surface of cells, which physically connect the extracellular matrix to the cytoskeleton and have long been speculated to mediate cell migration. (korea.ac.kr)
  • The predictive power of this relationship is comprehensively validated by disrupting nonfocal adhesion proteins (α-actinin, F-actin, and myosin II) and subcellular organelles (mitochondria, nuclear DNA, etc.) not known to affect either focal adhesions or cell migration. (korea.ac.kr)
  • In addition to its role in controlling cell cycle progression, the tumor suppressor protein p53 can also affect other cellular functions such as cell migration. (rupress.org)
  • These studies have served to highlight the role of AND-34/BCAR3 and p130Cas in cell adhesion and migration signaling pathways. (molvis.org)
  • In order to be effective and integrated to the receiving area, the bone graft is required to allow a strong cell adhesion, interacting with several molecules to induce migration, differentiation, and thus the mineralization of the new bone on the graft. (bvsalud.org)
  • A lager expansion of the cell area accompanied by a greater development of actin cytoskeleton and talin1, a greater adhesion strength of CTEs to each disk, and higher gene expression level of focal adhesion protein were observed with the mirror-polished surface than the mechanically polished surface both Ti and Ce-TZP/Al 2 O 3 disks. (alliedacademies.org)
  • The cell shape is determined by the interplay between the dynamics of the actin cytoskeleton, cell adhesions and the cell membrane towards the extracellular surface. (nusl.cz)
  • Goreczny, G.J., Forsythe, I. and Turner, C.E. (2018) Hic-5 Regulates Fibrillar Adhesion Formation to Control Tumor Stromal Matrix Remodeling through Interaction with Tensin 1. (upstate.edu)
  • The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. (thermofisher.com)
  • Finally, CXCL1 triggered the transcriptional activities of c-Jun (which regulates the expression of proinflammatory proteins) in OASFs and RASFs. (researchsquare.com)
  • Together with dynactin, dynein regulates centrosomal orientation to establish and maintain cell polarity, controls focal adhesion turnover and anchors microtubules at the leading edge. (biologists.com)
  • The effects of tenascin-C combined with its location around the wound bed suggest that this protein regulates fundamental processes of tissue repair by limiting the extent of matrix deposition and contraction to fibrin-FN-rich matrix in the primary wound area. (ox.ac.uk)
  • We have previously shown that the protein scleraxis directly regulates the genes involved in cardiac fibrosis. (sbrc.ca)
  • Mouse embryonic fibroblasts (MEF) were transfected with different mutations of the adaptor protein vinculin and FRAP experiments were performed to analyze the influence of the mutations on the incorporation of vinculin into focal adhesions (FAs). (rapp-opto.com)
  • Here we use quantitative microscopy to characterize descriptors of focal adhesion and cell motility for mouse embryonic fibroblasts and human fibrosarcoma cells, across a wide range of matrix compliance and following genetic manipulations of focal adhesion proteins (vinculin, talin, zyxin, FAK, and paxilin). (korea.ac.kr)
  • In this study, we show that p53 deficiency in mouse embryonic fibroblasts cultured in three-dimensional matrices induces a switch from an elongated spindle morphology to a markedly spherical and flexible one associated with highly dynamic membrane blebs. (rupress.org)
  • Mirror-polished Ce-TZP/Al 2 O 3 might contribute to the success of implant therapy by its strong adhesion strength to gingival connective tissues with the developed focal contact-cytoskeleton complex. (alliedacademies.org)
  • Although focal adhesions are similar, in terms of molecular composition, to nascent adhesions and focal complexes, several adhesion proteins appear to change as focal adhesions evolve in response to tension, e.g., zyxin and vinculin . (cellmigration.org)
  • In this image, we show that scleraxis causes an increase in the level of a protein called vinculin (green stain), particularly in small points at the cell edge called focal adhesions - a hallmark of cells that have converted to myofibroblasts. (sbrc.ca)
  • Thus, deposition of additional ECM components, expression of co-receptors for ECM, cleavage of adhesive proteins, and the architecture of the ECM microenvironment are different mechanisms for modulating cell responses to fibronectin matrix. (princeton.edu)
  • We found that SH, M-Gly, and P334 have significant effects on the wound healing process in human keratinocytes and these effects were mediated by activation of focal adhesion kinases (FAK), extracellular signal-regulated kinases (ERK), and c-Jun N-terminal kinases (JNK). (elsevierpure.com)
  • PSTPIP2 interacts with several signaling regulators, including PEST family protein tyrosine phosphatases (PEST-PTPs) and inositol. (nusl.cz)
  • Thus, this study defines the location of key residues in TM-A with respect to the lipid bilayer, describes the conformation of TM-A in a biomembrane mimic, presents a peptide-bilayer model useful in the consideration of local protein folding in the microsomal desaturases, and presents a model of arginine and tyrosine control of transmembrane protein stability and insertion. (shengsci.com)
  • Proto-oncogene proteins fes are protein-tyrosine kinases with a central SH2 DOMAIN. (bvsalud.org)
  • SPARC is produced by fibroblasts, capillary endothelial cells, platelets and macrophages, especially in areas of tissue morphogenesis and remodeling (3, 7). (rndsystems.com)
  • This phenomenon, termed fouling, is then followed by a series of undesired reactions involving activation of complement or the coagulation cascade and adhesion of leukocytes and platelets leading to thrombus formation. (ibecbarcelona.eu)
  • Compared with an unmodified glass surface, the grafting of polymer brushes minimizes the adhesion of platelets and leukocytes and prevents the thrombus formation. (ibecbarcelona.eu)
  • Mirror-polished surface of ceria-stabilized zirconia/alumina nanocomposite enhancement in the adhesion strength of human gingival fibroblasts. (alliedacademies.org)
  • Membrane adaptor proteins are proteins associated with cellular membranes that do not themselves serve as receptors. (nusl.cz)
  • In this thesis, I sought to describe selected membrane adaptor proteins and their roles in inflammation and regulation of hematopoiesis in mouse models using a reverse genetics approach. (nusl.cz)
  • Defects in the signaling pathways associated with cell adhesion provide the basis for cell transformation and cancer cell metastasis, various developmental defects and cardiovascular disease. (upstate.edu)
  • LPA activates at least six G-coupled protein receptors (LPA 1-6 ) stimulating different signaling pathways through heterotrimeric G proteins such as G i/0 , G 12/13 , G q/11 , and G s . (hindawi.com)
  • It is a highly dynamic process that leads to an asymmetric distribution of cytoskeletal networks, cell organelles, protein complexes and signalling pathways, which is reflected in the typical polarised cell shape. (nusl.cz)
  • Crystal structure shows that residues implicated in cell binding, inhibition of cell spreading and disassembly of focal adhesions cluster on one face of SPARC, while a collagen binding epitope and an N-glycosylation site are opposite this face (6). (rndsystems.com)
  • In turn, such forces cause pathologic changes to the heart, including promoting the conversion of cardiac fibroblasts into myofibroblasts, which secrete high levels of proteins like collagen that serve to stiffen the heart and resist these forces. (sbrc.ca)
  • In effect, this cell is attaching more firmly to its surroundings via these focal adhesions in preparation to make more collagen proteins. (sbrc.ca)
  • Several molecules are involved in the process of cell adhesion to bone grafts, highlighting the role of integrins, the focal adhesion mechanism, the influence of the collagen matrix, and the activity of alkaline phosphatase in bone matrix formation. (bvsalud.org)
  • To occur the adhesion of CAMs to the biomaterial, it is necessary their interaction with some components of extracellular matrix, such as proteoglycans, collagen and proteins 23 . (bvsalud.org)
  • We have found that these multi-domain proteins bind numerous structural and signaling proteins including kinases, phosphatases and Rho family GTPase regulators and effectors. (upstate.edu)
  • In mice, missense mutations in the Pstpip2 gene causing loss of PSTPIP2 protein lead to the development of autoinflammatory disease chronic multifocal osteomyelitis (CMO) characterized by sterile inflammatory lesions in the bones and adjacent soft tissue. (nusl.cz)
  • and microtubule targeting, which may contribute to adhesion disassembly. (cellmigration.org)
  • Development of the non-protruding regions requires the scaffold protein RACK1, which promotes adhesion-mediated activation of ERK2. (nusl.cz)
  • We prepared Titanium (Ti) and Ce-TZP/Al 2 O 3 disks with different surface roughness, namely, mirrored and machined surfaces, on which HGF-1 were cultured, and then the biological parameters of the cultured cells, including cell morphology and gene expression of focal adhesion proteins were assessed. (alliedacademies.org)
  • The resistance of cells on a biomaterial to compressive, tensile, or shear forces largely depends on the cell adhesion strength [ 8 ]. (alliedacademies.org)
  • The particular type of intermediate filaments attached tothe desmosomes depends on the cell type: they are keratin filaments in mostepithelial cells, for example, and desmin filaments in heart muscle cells.The junction hasa dense cytoplasmic plaque composed of a complex of intracellular anchorproteins (plakoglobin and desmoplakin) that are responsible for connecting thecytoskeleton to the transmembrane adhesion proteins. (ostatic.com)
  • This results in a severe blistering of the skin, withleakage of body fluids into the loosened epithelium.Anchoring Junctions Formed by Integrins Bind Cells to theExtracellular Matrix: Focal Adhesions andHemidesmosomesSome anchoring junctions bind cells to the extracellular matrix rather than toother cells. (ostatic.com)
  • The transmembrane adhesion proteins in these cell-matrixjunctions are integrins a large family of proteins distinct from the cadherins.Focal adhesions enable cells to get a hold on the extracellular matrix through integrins that link intracellularly to actin filaments. (ostatic.com)
  • In combination with a 100x objective, a spot size of approximately 5 µm in diameter was illuminated, allowing bleaching of single focal adhesions in living cells. (rapp-opto.com)
  • When stimulated with MET-positive HCC cells in vitro, MET-CAR.CD28ζ T cells demonstrated a higher level of cytokine release and expression of programmed cell death protein 1 (PD-1) than MET-CAR.4-1BBζ T cells. (bvsalud.org)
  • Cells on fibrin-FN+tenascin-C redistribute their actin to the cell cortex, downregulate focal adhesion formation, and do not assemble a FN matrix. (ox.ac.uk)
  • These adhesions are usually physically linked to large, readily visualized actomyosin bundles [13] , and their appearance correlates inversely with motility, such that they are conspicuously absent in cells migrating within a three-dimensional (3D) substrate. (cellmigration.org)
  • Fibrillar adhesions represent an extreme in the maturation of adhesions and are not generally seen in migrating cells. (cellmigration.org)
  • Podosomes are ring-shaped adhesions often found in fast-moving cells, such as macrophages. (cellmigration.org)
  • Cell adhesion and spreading on the extracellular matrix is a morphogenetic process in which cells initially spread isotropically from the point of first contact and then spontaneously break their radial symmetry and develop a migratory polarity with spatially separated protruding cell front and non-protruding cell rear. (nusl.cz)
  • Communication-deficient cells (the SKHep1 cell line) were stably transfected with a plasmid containing cDNA which encodes the major gap junction protein of rat liver, connexin32. (shengsci.com)
  • Western blot analysis and in situ hybridization confirmed the presence of AND-34 RNA and protein in lens epithelial cells, particularly at the lens equator. (molvis.org)
  • These results demonstrate the loss of AND-34 dysregulates focal adhesion complex signaling in lens epithelial cells and suggest that AND-34-mediated signaling is required for maintenance of the structural integrity of the adult ocular lens. (molvis.org)
  • Although the aetiology of these disorders remains unknown, in this review we analyse the pathogenic mechanisms by cell of interest (fibroblast, fibrocyte, myofibroblast, endothelial and alveolar epithelial cells and immune competent cells). (ersjournals.com)
  • Myelinating Schwann cells form a myelin sheath around a single axon and express high levels of myelin-related proteins and messenger RNA (mRNA). (medscape.com)
  • These cell adhesion molecules (CAM) will mediate the contact between two cells or between cells and the extracellular matrix, an essential process to the success of the implant. (bvsalud.org)
  • Some of these molecules are glycoproteins expressed on the cell surface, socalled cell adhesion molecules (CAM), which mediate either the contact between two cells or the contact among cells and the extracellular matrix, being therefore of fundamental importance for adhesion 3 . (bvsalud.org)
  • Mutations in these proteins may alter the function of renal cilia, which enable tubular cells to sense flow rates. (msdmanuals.com)
  • For the quantification of the real protein exchange dynamics within focal adhesions without overlying cytosolic diffusion artifacts, the FRAP technique was combined with TIRF (total internal reflection fluorescence) microscopy. (rapp-opto.com)
  • Focal complexes are adhesions in the early stages of maturation. (cellmigration.org)
  • Instead, they propagate or modify the signals of these receptors by recruiting other signaling and regulatory proteins and arranging them into supramolecular complexes. (nusl.cz)
  • These adhesions generate signals that activate Rac, promoting actin polymerization and preventing myosin II engagement in the lamellipodium. (cellmigration.org)
  • Rho activation is thought to generate focal adhesions and actin bundling due to increased myosin II activity. (cellmigration.org)
  • They are larger than nascent adhesions, depend on myosin II for their formation and maintenance, and reside at the boundary of the lamellum and lamellipodium [10] , [11] . (cellmigration.org)
  • the tadpoles showed reduced expression of cα(E)-catenin , small muscle protein, dystrophin , and myosin light chain genes. (biomedcentral.com)
  • Myosin-binding protein C stabilizes, but is not the sole determinant of SRX myosin in cardiac muscle. (tamu.edu)
  • It was found that the nanoscale gratings and pillars could facilitate focal adhesion elongation by providing anchoring sites, and the nanogratings could orient focal adhesions and nuclei along the nanograting direction, depending on not only the feature size but also the spacing of the nanogratings. (cdc.gov)
  • We previously showed that autophosphorylation occurs early during protein biosynthesis, hampering complete maturation of SADDAN receptors. (univr.it)
  • Likewise,when cultured fibroblasts migrate on an artificial substratum coated withextracellular matrix molecules, they also grip the substratum at focaladhesions, where bundles of actin filaments terminate. (ostatic.com)
  • Figure 1(a) is the pixelated image of fluorescent protein single molecules attached to a coverslip. (fsu.edu)
  • The positions of the individual molecules cannot be precisely determined because of their overlapping point-spread functions, but the center of protein molecules can be temporally resolved with high accuracy (Figure 1(b)) using PALM or similar techniques. (fsu.edu)
  • PALM and FPALM were developed using photoactivatable or photoconvertible fluorescent proteins (tandem dimer Eos and photoactivatable GFP, PA-GFP) as the switchable probes, whereas STORM was originally published using the synthetic carbocyanine dyes, Cy3 and Cy5, to label short DNA molecules. (fsu.edu)
  • Besides, TTK was involved in metabolic processes and regulated cell adhesion molecules pathway. (bvsalud.org)
  • This paper is a systematic review of the literature on the mechanisms of cell adhesion to bone grafts associated to nanotechnology, describing the importance and the role of those molecules in the adhesion and thus in tissue regeneration. (bvsalud.org)
  • The main reported mechanisms involve cell adhesion molecules (CAMs) and extracellular matrix components. (bvsalud.org)
  • Notwithstanding, to achieve the effectiveness and integration of the grafted tissue to the receptor site, it is necessary a strong cell adhesion, so that it demands many molecules interaction to induce cell differentiation and the bone matrix mineralization formed onto the graft. (bvsalud.org)
  • Osteonectin (ON) also known as secreted protein acidic and rich in cysteine (SPARC) or basement-membrane protein 40 (BM-40) is a protein that in humans is encoded by the SPARC gene. (wikipedia.org)
  • This work is focused on Neuron navigator 3 (NAV3), a protein that binds to + ends of microtubules (MTs), participates in their stabilization, and is able to mediate crosstalk between MTs and the actin network. (nusl.cz)
  • My lab uses a multi-faceted approach combining biochemistry, cell and molecular biology and various high-end microscopy techniques along with mouse knockout and tumor models to determine the molecular organization of the proteins that are involved in cell adhesion and thereby understand how they each contribute to cell behavior in vivo. (upstate.edu)
  • Central to the recent dramatic rise in the use of fluorescence microscopy in cell biology has been the development of genetically-encoded fluorescent proteins that act as endogenous labels to enable virtually any protein or peptide to become a fluorescent homing beacon for imaging and analysis. (fsu.edu)
  • Mutations in the fibroblast growth factor receptor 3 (FGFR3) gene cause chondrodysplasias. (univr.it)
  • Inhibiting Fibroblast Mechanotransduction Modulates Severity of Idiopathic Pulmonary Fibrosis. (stanford.edu)
  • In this paper, we demonstrate that physical force (cell stretch) requires scleraxis to facilitate the fibroblast to myofibroblast conversion process. (sbrc.ca)
  • Through integrated analysis of single cell chromatin landscapes and gene expression states, coupled with spatial transcriptomic profiling, we are able to impute fibroblast epigenomes with temporospatial resolution. (stanford.edu)
  • Based on a better understanding of the basement membrane zone (BMZ) and the genes responsible for its components, newer treatments (eg, gene or protein therapy) may provide solutions to the skin fragility found in patients with epidermolysis bullosa. (medscape.com)
  • We, herein, engineered nanotopographies of various shapes (gratings and pillars) and dimensions (feature size, spacing and height), and thoroughly investigated cell spreading, focal adhesion organization and nuclear deformation of human primary fibroblasts as the model cell grown on the nanotopographies. (cdc.gov)
  • PXN is a focal adhesion-associated protein playing a key role in cytoskeletal organization. (univr.it)
  • Now a large and ever increasing number of genetic subtypes has been described, and major advances in molecular and cellular biology have clarified the understanding of the role of different proteins in the physiology of peripheral nerve conduction in health and in disease. (medscape.com)
  • Throughout cell-material interaction, adhesion is influenced by the features of a biomaterial surface, comprising topography and physicochemical properties. (alliedacademies.org)
  • Most of the identified proteins are connected with the initial events of foreign body reaction towards biomaterial (coagulation cascade proteins, complement component, and inflammatory proteins). (ibecbarcelona.eu)
  • In addition, several proteins that are not previously linked with blood-biomaterial interaction are presented and discussed. (ibecbarcelona.eu)
  • Compared with continuous nanogratings, discrete nanopillars tended to disrupt the formation and growth of focal adhesions and thus had less profound effects on nuclear deformation. (cdc.gov)
  • It was also shown that IDPs from 45S5.2B BG could enhance in vitro HUVEC tubule formation and secretion of interleukin 6 (IL6) and the basic fibroblast growth factor (bFGF). (rsc.org)
  • Whereas the formation of focal adhesions requires actomyosin-mediated contractile force mediated by RhoA , podosome formation is stimulated by a local loss of contractility and the recruitment of negative regulators of RhoA, such as p190RhoGAP [16] . (cellmigration.org)
  • The main part of the work focused on the role of the membrane adaptor protein PSTPIP2 in suppressing inflammation. (nusl.cz)
  • A number of membrane proteins act via binding at the water/lipid bilayer interface. (shengsci.com)
  • Mitochondrial membrane biogenesis: phospholipids and proteins go hand in hand. (tamu.edu)
  • Our study delineated the relationships between focal adhesions, nucleus and cell function and highlighted that the nanotopography could regulate cell phenotype and function by modulating nuclear deformation. (cdc.gov)
  • However, whether clustering of these molecular components into focal adhesions is actually required for these proteins to regulate cell motility is unclear. (korea.ac.kr)
  • Although nanotopography has been shown to be a potent modulator of cell behavior, it is unclear how the nanotopographical cue, through focal adhesions, affects the nucleus, eventually influencing cell phenotype and function. (cdc.gov)
  • These properties will act mediating the action of cell adhesion proteins, regulating cell behavior and causing tissue regeneration. (bvsalud.org)
  • START domain-containing proteins (STARDs) are responsible for lipid metabolism. (medsci.org)
  • This protein is synthesized by macrophages at sites of wound repair and platelet degranulation, so it may play an important role in wound healing. (wikipedia.org)
  • The phosphate is bound to the protein backbone through phosphorylated serine or threonine amino acid residues. (wikipedia.org)
  • The 302 amino acid (aa), 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, and a C-terminal extracellular calcium (EC) binding domain with two EF-hand motifs (1‑5). (rndsystems.com)
  • Therefore, we designed a protein-free dispersion media containing phospholipids and amino acids in an isotonic balanced electrolyte solution, a solution for nasal and olfactory transport (SNOT). (cdc.gov)
  • Integrated spatial multiomics reveals fibroblast fate during tissue repair. (stanford.edu)
  • We employ a stented wound model that recapitulates human tissue repair kinetics and multiple Rainbow transgenic lines to precisely track fibroblast fate during the physiologic response to skin injury. (stanford.edu)