• Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta -catenin levels (2). (novusbio.com)
  • The CTNNB1 gene provides instructions for making a protein called beta-catenin. (medlineplus.gov)
  • The beta-catenin protein is also involved in cell signaling as an essential part of the Wnt signaling pathway. (medlineplus.gov)
  • Certain proteins in this pathway attach (bind) to beta-catenin, which triggers a multistep process that allows the protein to move into the cell nucleus. (medlineplus.gov)
  • Once in the nucleus, beta-catenin interacts with other proteins to control the activity (expression) of particular genes. (medlineplus.gov)
  • They change single protein building blocks (amino acids) in the beta-catenin protein. (medlineplus.gov)
  • These mutations lead to an abnormally stable beta-catenin protein that is not broken down when it is no longer needed. (medlineplus.gov)
  • They cause the beta-catenin protein to be turned on all the time (constitutively active), which leads to the abnormal activation of certain genes. (medlineplus.gov)
  • This active beta-catenin protein promotes Wnt signaling longer than normal, which leads to the unchecked proliferation of kidney cells and tumor development. (medlineplus.gov)
  • Not much is known about RAPGEF5 protein, but we know it is involved in the canonical Wnt pathway, in the transportation of beta-catenin into the nucleus [2]. (biologists.com)
  • When Wnt is active, a cascade of chemical reactions prevent the degradation of beta-catenin in the cytoplasm, allowing it to bind to a transporter protein to enter the nucleus. (biologists.com)
  • Our current model suggests that in response to Rap-GDP conversion to Rap-GTP by RAPGEF5, beta-catenin can dissociate from the transporter protein. (biologists.com)
  • This frees beta-catenin, allowing it to interact with DNA-binding proteins to alter gene expression. (biologists.com)
  • This gene encodes a high-affinity dickkopf homolog 1 (DKK1) transmembrane receptor that functionally cooperates with DKK1 to block wingless (WNT)/beta-catenin signaling. (nih.gov)
  • We demonstrate that while Rspo3 from myofibroblasts maintains colonic Lgr5 + cells during homeostasis, during injury its main function is not to maintain Lgr5 + cells but to interact with more differentiated cells that express Lgr4 but not Lgr5 and are able to regain expression of Wnt target genes and generate new crypts. (nature.com)
  • Next, I thoroughly demonstrated that the 10 candidate target genes were substantially suppressed by expression of the relevant miR-BARTs, as measured by 3'UTR-containing firefly luciferase (FLuc) expression, mRNA and protein levels, and knockdown of seven of the 10 candidate genes could suppress apoptosis, mimicking the effects of relevant miR-BARTs. (duke.edu)
  • On the other hand, in order to identify miR-BARTs that can activate the Wnt signaling pathway, I analyzed the PAR-CLIP data set of C666 cells and discovered nine anti-Wnt signaling targets of miR-BARTs, including seven novel genes and two pro-apoptotic genes identified above. (duke.edu)
  • Using FLuc 3'UTR indicator assays, I proved that the 3'UTRs of all seven newly identified anti-Wnt signaling genes were indeed targeted by the relevant miR-BARTs identified by PAR-CLIP. (duke.edu)
  • We have learned that genes in mammalian cells are transcribed into messenger RNAs (mRNAs), which are to be translated into polypeptides (proteins). (intechopen.com)
  • For example, steroid and thyroid hormones directly bind to nuclear receptors, which induce expression of specific genes. (intechopen.com)
  • In other words, expression of specific genes might have relevance to pathogenesis of diseases. (intechopen.com)
  • To date, 1527 human miRNAs have been identified (Sanger miRBase 18 http://www.miRbase.org/index.shtml), forming less than 1% of all human genes, potentially regulating more than 10% of all protein coding genes [ 1 ]. (intechopen.com)
  • Key genes are color coded based on regional expression from fate map in (E). (E) Enriched expression of Sox17 -regulated transcripts. (xenbase.org)
  • in this tissue they found upregulation of Wnt signaling-related genes compared to control tissues. (alzforum.org)
  • Cells treated with the inducer had less than 10 percent of the normal levels of progranulin and altered expression of 153 other genes, compared to untreated cells. (alzforum.org)
  • Among the dementia-linked genes with altered expression were glycogen synthase kinase 3β (GSK-3β) protein phosphatase 2 A (PP2A), and APC, which are scaffolds mediating presenilin-1/β-catenin interactions. (alzforum.org)
  • The Popeye domain containing (POPDC) genes encode sarcolemma-localized cAMP effector proteins. (imperial.ac.uk)
  • Our data rule out a model suggesting that xKaiso is a direct repressor of Wnt signalling target genes in early Xenopus development via binding to promoter-proximal CTGCNA sequences as part of a xTcf3 repressor complex. (biologists.com)
  • In Drosophila, signal transduction from Wingless stabilizes cytosolic Armadillo, which then forms a bipartite transcription factor with the HMG-box protein Drosophila Tcf (dTcf) and activates expression of Wingless-responsive genes. (duke.edu)
  • A 2010 study comparing gene expression of cells expressing CD207 (a marker of Langerhans cells) in LCH lesions with epidermal CD207+ control cells identified differential expression of more than 2000 genes between these 2 subsets. (medscape.com)
  • Wnts are a family of genes that have been implicated in many human tumours, so far mainly studied in colorectal cancer where activated Wnt signalling occurs in a vast majority of tumours. (lu.se)
  • Most changes were subtle, but frequently the genes with altered expression were involved in basal cellular functions such as cell division, cell cycle regulation, apoptosis and cell signalling. (lu.se)
  • Some alterations were restored when the cells were transferred back to serum-containing medium, indicating that expression of these genes was controlled by components in serum. (lu.se)
  • Among these were, for example, several genes within the Wnt signalling pathway. (lu.se)
  • The gene LYPD6B encoding the protein is located on chromosome 2 in humans. (wikipedia.org)
  • Hypermethylation of the gene and a subsequent decreased expression has been demonstrated as one of the contributors to the invasive capacity of cancer cells in melanoma. (wikipedia.org)
  • CTNNB1 gene mutations in Wilms tumor result in an overly active protein. (medlineplus.gov)
  • MiRNAs are small non-coding RNAs which regulate gene expression by binding to complementary sequences in mRNAs. (duke.edu)
  • Moreover, MLLT1 -mutant tumours show an increase in MYC gene expression and HOX dysregulation. (nature.com)
  • Gene expression must be appropriately maintained to regulate development, differentiation, and proliferation of cells. (intechopen.com)
  • Imbalances or disturbances in gene expression are sometimes deleterious for living things. (intechopen.com)
  • Major factors that may contribute to the proliferation of the leiomyoma are the decrease in the protein CCN5 and the expression of the gene HBP1--both of which are components of the Wnt signaling pathway, a fundamental pathway in normal development and in disease. (tufts.edu)
  • MiRNAs, short (18-24 nucleotides) non-coding RNAs, are derived from long transcripts pri-miRNAs and pre-miRNAs [ 26 - 30 ].By targeting 3' untranslated regions (3'UTRs) of cognate mRNAs, miRNAs post-transcriptionally regulate gene expression and induce translational repression [ 29 , 30 ]. (intechopen.com)
  • Sox17 and β-catenin co-occupy Wnt-responsive enhancers to govern the endoderm gene regulatory network. (xenbase.org)
  • In some cases, Sox17 and β-catenin synergistically activate transcription apparently independent of Tcfs, whereas on other enhancers, Sox17 represses β-catenin/Tcf-mediated transcription to spatially restrict gene expression domains. (xenbase.org)
  • This activates a promoter that causes the GFP reporter gene to be expressed and produce proteins that fluoresce under a specific wavelength. (biologists.com)
  • What does this gene/protein do? (cancerindex.org)
  • What pathways are this gene/protein implicaed in? (cancerindex.org)
  • Search the gene expression profiles from curated DataSets in the Gene Expression Omnibus (GEO) repository. (cancerindex.org)
  • Rodent primary hepatic stellate cells (HSCs), mouse liver tumor-initiating stem cell-like cells (TICs), and human hepatocellular carcinoma (HCC) cell lines were exposed to Wnt signaling inhibitors and changes in gene expression patterns were analyzed. (nih.gov)
  • The dying neurons possess one good progranulin gene, but do not make enough of the protein. (alzforum.org)
  • The roles of b-catenin are 'classically' defined: as an adhesion protein and as a signaling protein, transducing extracellular signals to the nucleus to modify gene expression. (chemdiv.com)
  • In order to restrict reporter gene expression to the desired cell type, we utilized the β-catenin dependent CTP4-promoter to restrict the expression of Firefly Luciferase and enhanced green fluorescent fusion protein (EGFPLuc) to cell lines with deregulated wnt signaling including SW480, LS174T, HepG2, Coga2 and Coga12. (uni-muenchen.de)
  • One of such pro-apoptotic gene families is the Secreted Frizzled-Related protein (sFRPs) family, an antagonist of the Wnt signalling pathway. (biomedcentral.com)
  • Expression system for standard: Secreted frizzled-related protein 5 is a protein that in humans is encoded by the SFRP5 gene. (innov-research.com)
  • Specifically, the WNT5A gene was hypermethylated at the promotor region in the diabetic cells and its protein was markedly repressed. (medscape.com)
  • The discovery does not affect gene sequence but entails specific DNA modifications altering gene expression - what are known as epigenetic alterations," he said. (medscape.com)
  • Materials and Methods: We have adapted the Burkitt's lymphoma line Ramos to a serum-free medium that supports long-term survival and studied gene expression changes that occurred during the adaptation process. (lu.se)
  • Affymetrix micro-array technology was used to identify gene expression alterations that had occurred during the adaptation. (lu.se)
  • TCDD for every step of the mechanism described for 2,3,7,8-TCDD carcinogenesis in humans including receptor binding, gene expression, protein activity changes, cellular replication, oxidative stress, promotion in initiation-promotion studies and complete carcinogenesis in laboratory animals. (who.int)
  • Role of the planar cell polarity gene Protein tyrosine kinase 7 in neural tube defects in humans. (cdc.gov)
  • Together, my results identified and validated a substantial number of novel targets of miR-BARTs involved in apoptosis and the Wnt signaling pathway, indicating that EBV may employ miR-BARTs to heavily target these two pathways to facilitate chronic infection. (duke.edu)
  • Aberrant expression and glycosylation of mucins contribute to tumour survival and proliferation in many cancers, which in turn activates numerous signalling pathways such as NF-kB, ERα, HIF, MAPK, p53, c-Src, Wnt and JAK-STAT, etc. (amrita.edu)
  • Further, there are signaling pathways that participate in regulation, such as Wnt channel, mitogen-activated protein kinase (MAPK) channel, bone morphogenetic protein (BMPs) channel, peroxisome proliferator activated-receptors (PPARs) channel, and Notch channel. (frontiersin.org)
  • Wnt/β-catenin signaling pathways were also examined. (researchsquare.com)
  • The WNT signaling pathways are evolutionarily conserved and implicated in multiple crucial physiological and pathophysiological events [ 6 ]. (oncotarget.com)
  • To date, three Wnt signaling pathways have been characterized including the canonical Wnt/β-catenin signaling, the noncanonical planar cell polarity pathway (Wnt/PCP) and the Wnt/Ca 2+ pathway [ 7 , 8 ]. (oncotarget.com)
  • Instead, we propose that mutual inhibition by Kaiso/TCF3 of their DNA-binding functions may be important in developmental or cancer contexts and acts as a regulatory node that integrates epigenetic and Wnt signalling pathways. (biologists.com)
  • All proteins were subjected to Ingenuity Pathway Analysis (IPA) to create 13 biological networks and 17 pathways of interacting protein clusters in human ortholog. (cdc.gov)
  • In human breast epithelial cells Wnt-5a activates the canonical b-catenin pathway as well as the non-canonical Ca2+/calmodulin and planar cell polarity (PCP) pathways. (lu.se)
  • Over-expression of COMP leads to activation of Notch which affects both Wnt/β-catenin and AKT pathways, already affected by COMP. (lu.se)
  • Thus, in the present study, involvement of Wnt signaling was investigated in DPN using Wnt signaling inhibitors namely LGK974 (Porcupine inhibitor), NSC668036 (Disheveled inhibitor) and PNU74654 (β-catenin inhibitor). (iasp-pain.org)
  • Intrathecal administration of Wnt signaling inhibitors (each at doses of 10 and 30 µM) in diabetic rats showed improvement in pain-associated behaviors (heat, cold & mechanical hyperalgesia) and nerve functions (motor, sensory nerve conduction velocities and nerve blood flow) by decreasing the expression of Wnt pathway proteins, inflammatory marker, matrix metalloproteinase 2 (MMP2), ER stress marker, glucose-regulated protein 78 (GRP78) and improving IENFD. (iasp-pain.org)
  • All these results signify the neuroprotective potential of Wnt signaling inhibitors in DPN. (iasp-pain.org)
  • Blockade of this pathway using Wnt inhibitors provided neuroprotection in experimental DPN in rats. (iasp-pain.org)
  • This study may provide a basis for exploring the therapeutic potential of Wnt inhibitors in DPN patients. (iasp-pain.org)
  • Utilizing a Wnt signaling FLuc reporter TOPflash which measures the Wnt signaling activation, I confirmed that expression of many miR-BARTs that target Wnt signaling inhibitors can indeed upregulate the Wnt signaling pathway. (duke.edu)
  • They further link to the activation of protein kinase C- (PKC-) induced generation of reactive oxygen species (ROS) [ 6 , 7 ], which further mediates the activation of downstream transcription factor nuclear factor kappa-light-chain enhancer of activated B cells (NF- κ B). Thus, the main treatments of DN refer to modulate glycemic and blood pressure through insulin and RAS inhibitors. (hindawi.com)
  • Although the senescent cells remain viable, they show typical changes with enlarged and flattened cell bodies, apoptosis resistance, increased activity of senescence-associated β -galactosidase (SA- β -gal), and upregulation of cyclin-dependent kinase (CDK) inhibitors including p16 INK4A , ARF proteins, and p21 [ 13 - 16 ]. (hindawi.com)
  • However, treatment with various approaches, including exogenous WNT5A methylation inhibitors and a nanoconjugate that inhibits WNT5A suppression, improved corneal epithelial wound healing as well as expression of the limbic epithelial stem cells. (medscape.com)
  • Similarly, depletion in the STRIPAK component STRIP1 affects activation of GCKIII kinases and cell cycle disruption through elevated expression of cyclin dependent kinase inhibitors p21 and p27, enhanced levels of which lead to a protective effect from therapeutic treatments and increased proliferation. (lu.se)
  • As a prototoxin, LYPD6B also belongs to the protein family of Ly-6/urokinase plasminogen activator receptor (Ly6/uPAR). (wikipedia.org)
  • Rspo proteins are secreted and can stabilize the effects of Wnt ligands by preventing ubiquitination and turnover of the Wnt receptor frizzled 9 , thereby dictating the size of the Lgr5 + stem cell pool by regulating self-renewal of Lgr5 + cells 10 . (nature.com)
  • M-CSF binds to the M-CSF receptor, which is promote the proliferation and differentiation of OC and the continuous expression of RANK. (frontiersin.org)
  • RANK binds to transmembrane protein nuclear factor-κB receptor activating factor ligand (RANKL) to activate RANK/RANKL, TRAF6/RANKL/MAPKs, TRAF6/ RANKL/NF-κB, Wnt/RANKL/RANKL, and JAK2/STAT3/RANKL. (frontiersin.org)
  • We examined the effects of SCD inhibition on Wnt signaling, including the expression and stability of low-density lipoprotein-receptor-related proteins 5 and 6 (LRP5 and LRP6), by immunoblot and quantitative polymerase chain reaction analyses. (nih.gov)
  • The encoded protein is a component of a membrane complex that modulates canonical WNT signaling through lipoprotein receptor-related protein 6 (LRP6). (nih.gov)
  • This protein has also been found to be a functional receptor for Coxsackievirus A10 and may be an alternative entry receptor for SARS-CoV-2. (nih.gov)
  • In the canonical Wnt/β-catenin signaling, a Wnt ligand binds to a cell surface receptor complex consisting of the members of Frizzled family and the transmembrane protein LRP 5 and 6 to prevent phosphorylation and degradation of β-catenin by the GSK3β/APC/Axin destruction complex. (oncotarget.com)
  • With these stably transduced cell lines, we performed a therapeutic target screen via siRNA-mediated knock-down of a number of potentially therapeutic targets within the wnt pathway - osteoprotegerin (OPG), Traf2 and Nck-interacting kinase (TNIK), SRY-related HMG-box (Sox2), protease-activated receptor 1 (PAR-1), β-catenin and transcription factor 4 (TCF4). (uni-muenchen.de)
  • LRRK2 interacts with key Wnt signaling proteins of the β-catenin destruction complex and dishevelled proteins in vivo and is recruited to membranes following Wnt stimulation, where it binds to the Wnt co-receptor low-density lipoprotein receptor-related protein 6 (LRP6) in cellular models. (open.ac.uk)
  • In normal breast epithelium, Wnt-5a is important for cell-ECM adhesion and activation of the collagen receptor DDR1. (lu.se)
  • In rodent HSCs and TICs, the Wnt effector β-catenin increased sterol regulatory element binding protein 1-dependent transcription of Scd, and β-catenin in return was stabilized by MUFAs generated by SCD. (nih.gov)
  • Speaking more specifically, β-catenin is an integral structural component of cadherin-based adhesive junctions as well as it a key nuclear effector of canonical in nucleus Wnt signaling. (chemdiv.com)
  • These MLLT1 mutations were associated with changes in binding to H3K9ac, evidence of HOX and MYC (MYC-C) expression dysregulation, and were often accompanied by CTNNB1 mutations and evidence of Wnt pathway activation. (nature.com)
  • We conclude that activating MLLT1 mutations accompanied by Wnt activation early in renal development result in the development of Wilms tumour. (nature.com)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • Progranulin mutations cause some forms of frontotemporal dementia, but exactly what the protein does and how it fails in disease are open questions. (alzforum.org)
  • Finally, a newly developed LRRK2 kinase inhibitor disrupted Wnt signaling to a similar extent as pathogenic LRRK2 mutations. (open.ac.uk)
  • Here, I present two studies utilizing the phenotypic and the target approaches, respectively, to demonstrate that miR-BARTs can inhibit apoptosis and activate the Wnt signaling pathway. (duke.edu)
  • This study is the first to show that VALD-3 induces apoptosis and cell cycle arrest in human breast cancer cells by suppressing Wnt/β-catenin signaling, indicating that it could be a potential drug for the treatment of breast cancer. (researchsquare.com)
  • N-SLIT2 amplifies reactive oxygen species production in response to the bacteria by activating p38 mitogen-activated protein kinase that in turn phosphorylates NCF1, an essential subunit of the NADPH oxidase complex. (elifesciences.org)
  • Wnt-3a is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes (1). (novusbio.com)
  • Wingless/Wnt signalling directs cell-fate choices during embryonic development. (duke.edu)
  • We show that Sox17 functionally interacts with the canonical Wnt pathway to specify and pattern the endoderm while repressing alternative mesectoderm fates. (xenbase.org)
  • VALD-3 also regulated the Wnt/β-catenin signaling pathway in breast cancer cells, inhibiting the activation of downstream molecules. (researchsquare.com)
  • b-Catenin is the central mediator of Wnt signaling and is frequently overexpressed in AML in which it is associated with poor prognosis. (haematologica.org)
  • The expression of Wnt-5a protein co-variates with that of Syk, a tyrosine kinase that is lost in up to 30% of invasive breast carcinomas and associated with poor prognosis. (lu.se)
  • Furthermore in the in vitro experiments, the tumor-selectivtiy of the CTP4-promoter was employed in the delivery of the cytotoxic protein diphteria toxin A (DTA) in colorectal cancer target cells. (uni-muenchen.de)
  • Objective: To evaluate the immunohistochemistry expression of the protein wnt-5a in oral epithelial dysplasia. (bvsalud.org)
  • Hematoxylin and eosin (HE) staining of tumor tissue specimens, along with immunohistochemistry (IHC), were conducted to evaluate the expression levels of Ki67, GRP78, N-cadherin, Vimentin, and ABCG2. (bvsalud.org)
  • Transmembrane protein 88 inhibits transforming growth factor-β1-induced-extracellular matrix accumulation and epithelial-mesenchymal transition program in human pleural mesothelial cells through modulating TGF-β1/Smad pathway. (nih.gov)
  • Transmembrane protein 88 exerts a tumor-inhibitory role in thyroid cancer through restriction of Wnt/β-catenin signaling. (nih.gov)
  • Transmembrane protein 88 (TMEM88) promoter hypomethylation is associated with platinum resistance in ovarian cancer. (nih.gov)
  • Here we demonstrate aberrant activation of Wnt/β-catenin signaling in bone sarcoma cells, involving an autocrine Wnt signaling loop with upregulation of specific Wnt ligands and receptors. (oncotarget.com)
  • Taken together, our findings establish the evidence that aberrant activation of Wnt/β-catenin pathway involving an autocrine Wnt singaling drives the proliferation of bone sarcoma cells, and identify the autocrine activation of the Wnt/β-catenin signaling as a potential novel therapeutic target for bone sarcomas. (oncotarget.com)
  • Sonic Hedgehog Signaling Affected by Promoter Hypermethylation Induces Aberrant Gli2 Expression in Spina Bifida. (cdc.gov)
  • and protein stabilization. (nih.gov)
  • This loop required MUFA inhibition of binding of Ras-related nuclear protein 1 (Ran1) to transportin 1 and reduced nuclear import of elav-like protein 1 (HuR), increasing cytosolic levels of HuR and HuR-mediated stabilization of mRNAs encoding LRP5 and LRP6. (nih.gov)
  • In rodent HSCs and TICs, we found SCD expression to be regulated by Wnt-β-catenin signaling, and MUFAs produced by SCD provided a forward loop to amplify Wnt signaling via stabilization of Lrp5 and Lrp6 mRNAs, contributing to liver fibrosis and tumor growth. (nih.gov)
  • β-catenin is a component of the complex cadherin complex whose stabilization is required to activate Wnt/β-catenin signaling. (chemdiv.com)
  • Studies have demonstrated that the re-expression of sFRPs, in particular sFRP4, is associated with a better prognosis, and that experimentally induced expression results in cell death. (biomedcentral.com)
  • Furthermore, patients with tumours expressing both Wnt-5a and Syk had a significantly better prognosis as compared with those displaying loss of either of or both proteins. (lu.se)
  • The protein is 183 amino acids long and its molecular mass is 20.656. (wikipedia.org)
  • Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. (novusbio.com)
  • These are high molecular weight glycosylated proteins located in the epithelial lining of several tissues and ducts. (amrita.edu)
  • One such molecular target of considerable interest given its frequent dysregulation in AML is Wnt/b-catenin signaling. (haematologica.org)
  • The molecular weight of proteins were 102, 88 and 80 kDa, so they were named α, β and γ-catenin. (chemdiv.com)
  • Comparison of sFRP4 expression in tumour biopsies revealed a positive trend between sFRP4 expression and tumour grade, with mucinous cyst adenocarcinomas exhibiting significantly decreased sFRP4 levels compared to mucinous borderline tumours. (biomedcentral.com)
  • We further examined sFRP4 expression in human ovarian tumours to assess if its expression could be correlated with clinico-pathological features consistent with a proposed role for loss being a contributor to chemoresistance. (biomedcentral.com)
  • Consequently, the increased metastatic potential of breast tumours with low Wnt-5a expression could be due to inactivation of DDR1 or enhanced NFAT activation. (lu.se)
  • In EBV latently infected epithelial cells, such as nasopharyngeal carcinoma (NPC) and gastric carcinoma (GaCa) cells, viral protein expression is low. (duke.edu)
  • Conclusion: The increased immuno-expression of the protein Wnt-5a observed in oral epithelial dysplasias, differ significantly from the normal pattern of healthy oral mucosa and shows a phenotypic change of epithelial cells as part of the events precursors of oral carcinogenesis. (bvsalud.org)
  • TMEM88 inhibits extracellular matrix expression in keloid fibroblasts. (nih.gov)
  • The production of extracellular matrix proteins, collagen, elastin, and proteoglycans presumably is due to a prolonged inflammatory process in the wound. (medscape.com)
  • The results demonstrate that zerumbone inhibits cell proliferation, migration and invasion, but represses the EMT process via inactivation of Wnt/β-catenin signaling pathway. (ajol.info)
  • The protein LYPD6 also leads to an increase in Wnt/β-catenin signaling. (wikipedia.org)
  • Furthermore, lithium chloride (Wnt activator) reversed the protective effect of STAT1 inhibition on VSMC calcification, while Dickkopf‑1 (Wnt inhibitor) exerted the opposite effect, suggesting that activation of the Wnt/β‑catenin signaling pathway was involved in STAT1‑mediated VSMC calcification. (spandidos-publications.com)
  • In conclusion, the present results indicated that exosomal STAT1 derived from HP‑treated HUVECs could promote VSMC calcification, and activation of the Wnt/β‑catenin pathway may be a potential mechanism of the VSMC calcification promoted by exosomes. (spandidos-publications.com)
  • TMEM88 exhibits an antiproliferative and anti-invasive effect in bladder cancer by downregulating Wnt/β-catenin signaling. (nih.gov)
  • Pharmacological Inhibition of Porcupine, Disheveled and β-catenin in Wnt Signaling Pathway Ameliorates Diabetic Peripheral Neuropathy in Rats. (iasp-pain.org)
  • Wnt/- Catenin Signaling Pathway Function in Uterine Leiomyoma: Potential for Therapeutic Intervention. (tufts.edu)
  • 2020), Sox17 and β-catenin co-occupy Wnt-responsive en. (xenbase.org)
  • Our findings establish Sox17 as a tissue -specific modifier of Wnt responses and point to a novel paradigm where genomic specificity of Wnt/β-catenin transcription is determined through functional interactions between lineage-specific Sox TFs and β-catenin/Tcf transcriptional complexes. (xenbase.org)
  • 1 Wnt/b-catenin is also known to drive the emergence and maintenance of leukemia stem cells in AML. (haematologica.org)
  • 2 Protein interactions are critical to the stability, localization and activity of b-catenin, and we recently performed the first proteomic analyses of the b-catenin interactome in myeloid cells. (haematologica.org)
  • In order to idenitfy appropriate cell lines in which to study b-catenin:WT1 interplay we first performed a screen of myeloid cell lines to examine b-catenin and WT1 protein expression. (haematologica.org)
  • We observed a statistically significant correlation between b-catenin and WT1 expression across 16 myeloid cell lines, with 50% (8/16) co-expressing b-catenin and WT1 to varying degrees ( Figure 1A , B). There was no particular association of this correlation with cell line morphology or genotype. (haematologica.org)
  • WT1 is an RNA-binding protein, 6 and β-catenin has also been shown to bind RNA, 7 so to confirm this interaction was not indirect via RNA binding we repeated WT1 Co-IP (with or without CHIR99021) after first confirming complete digestion of RNA through RNase A pre-treatment of cell lysates ( Figure 1E ). (haematologica.org)
  • As shown in Figure 1F-H, the b-catenin:WT1 interaction remained in K562, HEL and KG-1 cells under both basal (DMSO) and stimulated (CHIR99021) Wnt signaling. (haematologica.org)
  • We further wanted to ascertain whether this protein interaction was direct using recombinant versions of purified b-catenin and WT1 protein but failed to detect any association ( Online Supplementary Figure S1B, C ). This suggested that perhaps the interaction is mediated through a post-translational modification, common partners such as WTX, 8 or cellular structures like DNA, given their well-documented roles in transcription. (haematologica.org)
  • A) Immunoblot of myeloid leukemia cell lines showing the relative level of b-catenin (~92 kDa) and WT1 (~50 kDa) protein, with b-actin (42 kDa) used to assess protein loading. (haematologica.org)
  • β-Catenin is a fascinating protein with many important cellular and developmental functions. (chemdiv.com)
  • β -catenin has many binding partners that mediate a diverse set of cellular functions, and the protein probably acts as a 'hub' on which many cellular signaling networks impinge. (chemdiv.com)
  • Wnt/β-catenin signaling is a branch of a functional network that is involved in a broad range of biological systems including stem cells, embryonic development and adult organs. (chemdiv.com)
  • Deregulation of components involved in Wnt/β-catenin signaling has been implicated in a wide spectrum of diseases including a number of cancers and degenerative diseases. (chemdiv.com)
  • This library represents a selection of drug-like compounds aimed at modulating protein-protein interaction (PPI) of β-catenin with different proteins involved in significant physiological processes. (chemdiv.com)
  • The Wnt pathway mediates the biological processes of the canonical or non-canonical pathway, depending on the involvement of β-catenin in signal transduction. (chemdiv.com)
  • The recently described role of the Wnt/β-catenin pathway in the regulation of immune cell infiltration of microenvironment tumor resumes interest because of its potential impact to rection of immune therapy. (chemdiv.com)
  • This article presents the roles of the Wnt/β-catenin pathway in cancer and current therapeutic strategies involving this pathway. (chemdiv.com)
  • Nussе and Varmus identified components of the Wnt/β-catenin pathway in 1982 in a study of oncogenic breast tumor viral diseases (MMTV) [1]. (chemdiv.com)
  • Activation of Wnt/β-catenin signaling with Wnt3a or GSK-3β inhibitor drives the proliferation of bone sarcoma cells, whereas downregulation of activated Wnt signaling with dnTCF4 or siLEF1 suppresses bone sarcoma proliferation and induces cell cycle arrest. (oncotarget.com)
  • In this issue, we generally review the mechanisms of cellular senescence in diabetic nephropathy, which involve telomere attrition, DNA damage, epigenetic alterations, mitochondrial dysfunction, loss of Klotho, Wnt/ β -catenin signaling activation, persistent inflammation, and accumulation of uremic toxins. (hindawi.com)
  • As proof of principle the siRNA-mediated knock down of β-catenin was verified on mRNA and protein level in LS174T cells. (uni-muenchen.de)
  • We assessed Wnt signaling activity by evaluating ß-catenin expression in mouse intestinal tissue. (bvsalud.org)
  • The Wnt signaling pathway promotes the growth and division (proliferation) of cells and helps determine the specialized functions a cell will have (differentiation). (medlineplus.gov)
  • Human bmMSCs were described in the late nineties as well [ 6 ] and at the same time a breakthrough study investigated the expression of typical cell surface markers and the proliferation and differentiation properties of human MSCs in more detail [ 7 ]. (hindawi.com)
  • also known as ASH1), neurogenic differentiation factor 1 (NeuroD1), yes-associated protein 1 (YAP1) and POU class 2 homeobox 3 (POU2F3). (cancerindex.org)
  • HBP1, an HMG Box transcription factor, is a cell cycle inhibitor and a transcriptional repressor of the Wnt signaling pathway. (tufts.edu)
  • Despite the co-expression, loss of Wnt-5a and Syk protein appear to be regulated at the translational and transcriptional levels, respectively. (lu.se)
  • The protein is expressed in the nervous system where it acts as an enhancer of the activity of the neurotransmitter acetylcholine certain α7-containing nicotinic acetylcholine receptors, which have a role in learning. (wikipedia.org)
  • We aim to study the coupling between LTCC and β adrenergic receptors in different cardiomyocyte microdomains, the distinct involvement of PKA and CAMKII (Ca2+/calmodulin-dependent protein kinase II) and explore how this functional complex is disrupted in heart failure.Methods:Global signaling between LTCCs and β adrenergic receptors was assessed with whole-cell current recordings and western blot analysis. (imperial.ac.uk)
  • Moreover, Al-positive samples exhibited a significant decrease in the number of apoptotic cells, as well as the expression of the anti-apoptotic molecule BCL-2. (bvsalud.org)
  • Depending on the cellular effects through which Wnt-5a mediates its function, reconstitution of Wnt-5a signalling might have future therapeutical implications. (lu.se)
  • We demonstrate that a direct interaction between the methyl-CpG-dependent transcription repressor Kaiso and xTcf3, a transducer of the Wnt signalling pathway, results in their mutual disengagement from their respective DNA-binding sites. (biologists.com)
  • Wnt signaling pathway has been investigated extensively for its diverse metabolic and pain modulating mechanisms and recently its involvement has been postulated in the development of neuropathic pain. (iasp-pain.org)
  • However, there are no reports as yet on involvement of Wnt signaling pathway in one of the most debilitating neurovascular complication of diabetes, i.e, diabetic peripheral neuropathy (DPN). (iasp-pain.org)
  • Perspective: This study emphasizes the involvement of Wnt signaling pathway in diabetic peripheral neuropathy (DPN). (iasp-pain.org)
  • We investigated the involvement of complement C1q and Wnt signaling in intestinal mucosal regeneration using a murine colitis model. (bvsalud.org)
  • Complement C1q-mediated Wnt signaling activation has recently been suggested to mediate tissue repair and mucosal regeneration. (bvsalud.org)
  • Wnt-5a signalling is thus involved in the activation of DDR1 as well as in hampering NFAT activity, both of which affect the migratory capacity of tumour cells. (lu.se)
  • SFRP5 is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. (innov-research.com)
  • The secreted Frizzled-Related Protein (sFRP) family is pro-apoptotic and also a negative modulator of the Wnt signalling cascade. (biomedcentral.com)
  • The expression of pro-apoptotic proteins and anti-apoptotic proteins in MCF-7 and MDA-MB-231 cells was investigated by Western blotting. (researchsquare.com)
  • Western blot analysis revealed that VALD-3 upregulated pro-apoptotic proteins (Bad and Bax), downregulated anti-apoptotic proteins (Bcl-2, Bcl-xl, survivin and XIAP) and increased the expression of cleaved caspase-3, cleaved caspase-8, Cyto-c and cleaved PARP. (researchsquare.com)
  • Recombinant Human Wnt-3a (Catalog # 5036-WN) induces alkaline phosphatase production by the MC3T3-E1 mouse preosteoblast cell line. (novusbio.com)
  • 3 This study identified Wilms tumor protein (WT1) as a putative novel interaction partner in myeloid cells. (haematologica.org)
  • The expression of Wnt-5a protein was evaluated as membranous, cytoplasmatic and/or nuclear. (bvsalud.org)
  • The cytoplasmic / nuclear expression of Wnt-5a was the most frequent, with the strong intensity being the most prevalent. (bvsalud.org)
  • Changes in LRRK2 expression affects pathway activity, while pathogenic LRRK2 mutants reduce both signal strength and the LRRK2-LRP6 interaction. (open.ac.uk)
  • Thus, decreased LRRK2-mediated Wnt signaling caused by reduced binding to LRP6 may underlie the neurodegeneration observed in PD. (open.ac.uk)
  • We found that Wnt-5a protein expression is reduced in many invasive breast carcinomas and that reduced Wnt-5a expression in the primary tumour strongly correlates with an increased risk of metastatic disease and shorter survival. (lu.se)
  • Restitution of Wnt-5a signalling in breast tumour cells confer better adhesion and ability to activate DDR1 as well as a less malignant-looking phenotype. (lu.se)
  • Inhibition of STAT1 expression in VSMCs using fludarabine or knockdown of STAT1 expression using small interfering RNA alleviated the calcification of VSMCs. (spandidos-publications.com)
  • LRRK2, therefore, bridges membrane and cytosolic components of Wnt signaling. (open.ac.uk)
  • Conversely, silencing of sFRP4 expression in the chemosensitive cell line resulted in a corresponding increase in chemoresistance. (biomedcentral.com)
  • Exosomes derived from HP‑HUVECs promoted the calcification of VSMCs, as assessed by Alizarin Red S staining, alkaline phosphatase activity assays, Ca content measurements and the increased expression of runt‑related transcription factor 2 and osteopontin. (spandidos-publications.com)
  • Stable cell lines containing this CTP4-driven EGFPLuc construct were established with the help of a lentiviral vector to monitor wnt activity by transgene expression. (uni-muenchen.de)
  • The results presented in this thesis thus imply that Wnt-5a contains metastasis-suppressing activity in breast cancer. (lu.se)
  • Given the ubiquitous nature of Sox TFs and Wnt signaling, this mechanism has important implications across a diverse range of developmental and disease contexts. (xenbase.org)
  • Protein expression was determined by western blot. (ajol.info)
  • This protein is present in many types of cells and tissues, where it is primarily found at junctions that connect neighboring cells (adherens junctions). (medlineplus.gov)
  • Stacked bar graphs showing different patterns of enriched spatial expression based on RNA-seq of dissected gastrula tissues (Blitz et al. (xenbase.org)
  • The results indicated that PPI administration decreased the levels of proteins associated with metastasis and markers of drug resistance in tumor tissues, impeding tumor growth and spread. (bvsalud.org)
  • We identify two populations of cells with active Wnt signaling: highly proliferative Lgr5 + /Axin2 + cells, as well as secretory Lgr5 − /Axin2 + cells. (nature.com)
  • We demonstrate that Rspo3 is essential for epithelial repair via induction of Wnt signaling in differentiated cells. (nature.com)
  • Thus, we find that endogenous Rspo3 signaling is a critical determinant of cellular fate within the crypt and stimulates rapid recruitment of differentiated cells for epithelial wound healing and crypt regeneration through induction of Wnt signaling. (nature.com)
  • This protein is active in cells that make up a part of the hair follicle known as the matrix. (medlineplus.gov)
  • As a result, the protein accumulates within cells. (medlineplus.gov)
  • After stimulating the cells with Wnt1, the researchers used an unbiased expression screen to examine changes to the transcriptome over periods ranging from two hours to three days. (alzforum.org)
  • Specifically, a variety of other cellular populations have been identified that possess phenotypic characteristics similar to Langerhans cells, including expression of CD207 and Birbeck granules. (medscape.com)
  • Western blot analysis and immunofluorescence experiments showed that PPI reduced the expression of GRP78, E-cadherin, N-cadherin, Vimentin, and ABCG2 in SR-HCC cells. (bvsalud.org)
  • Overall, Wnt-5a is a new corneal epithelial wound healing stimulator that can be targeted to improve wound healing and stem cells in the diabetic cornea," write Ruchi Shah, PhD, of the Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, California, and colleagues. (medscape.com)
  • 10] Interestingly, this analysis found no differences in expression of proliferation markers between these subsets of CD207+ cells, consistent with the hypothesis that LCH may be a disease of abnormal cellular accumulation. (medscape.com)
  • In this compiled work, we elaborate on the effect of protein expression alterations and their resulting effect on recurrence capability of cells. (lu.se)
  • Further analyses seek to define the Wnt signaling mechanisms and are focused on our lab's unique organotypic model of leiomyoma, in which human fibroid tumors are grown in immune-compromised mice. (tufts.edu)
  • Expression of Lgr5 is controlled by Wnt signaling, which plays a critical role for stem cell turnover in the gastrointestinal tract 2 . (nature.com)
  • We have shown that in the stomach, stem cell homeostasis is regulated by Wnt and Rspo secreted by stromal myofibroblasts 11 . (nature.com)
  • The finding of Wnt-5a as a new diabetic corneal marker regulating wound healing and stem cell function may have implications for other diabetes complications involving impaired wound healing, including diabetic foot ulcers, as they share similar neurovascular, sensory, and immunological compromise with diabetic eye disease, Shah and colleagues say. (medscape.com)