Protein expression of cyclin dependent kinase inhibitors. Medical search. Frequent questions

The expression of genes through miRNA is regulated by endogenous regulatory pathways, resulting in high stability and biocompatibility ( 5 ). (spandidos-publications.com)
The variety of keratin genes differentially expressed suggests that these proteins may however have additional functions. (exposed-skin-care.net)
Thus, MRs comprise typically a small number of TF-encoding genes (and their products) that control a disproportionate level of gene expression, giving rise to distinct molecular phenotypes associated with a particular disease. (aging-us.com)
Since the expression of genes defining discrete phenotypes is highly coordinated, application of reverse engineering algorithms to transcriptome datasets allows interpreting transcriptional networks by defining MRs and their associated regulons and gene circuits. (aging-us.com)
Tumor suppressor genes encode proteins that normally provide negative control of cell proliferation. (medscape.com)
Subsequently released E2F factors mediate expression of pro-proliferative genes including cyclin E and Cdc25A. (shu.edu)
The expression of further E2F target genes, as cyclin A, facilitates progression through S phase. (shu.edu)
Expression profiling of both pathogen effector genes and host genes involved in immunity allows us to suggest distinct mechanisms of effector-mediated susceptibility and reveals interesting Hpa effectors for detailed mechanistic investigation in future experiments. (prelekara.sk)
By comparing the p53 protein level and its ability to transactivate target genes Waf1/Cip1 and mdm2 in both T22 and NIH3T3 cells irradiated with u.v., a discordance between the p53 protein level and its transcriptional activity was observed. (ox.ac.uk)
The observed lack of correlation between the elevated p53 and beta-galactosidase and expression in u.v. irradiated cells strongly indicates that the ability of p53 to transactivate its target genes is not simply correlated to its protein level. (ox.ac.uk)
One of the activated genes is an inhibitor of cyclin-dependent kinases. (biossusa.com)
To this effect our laboratory has focused on specificity protein (Sp) transcription factors (TFs) since Sp1 and additional Sp TFs regulate manifestation of multiple genes that are important for malignancy cell growth and survival and Sp TFs can be efficiently targeted by numerous anti-cancer providers. (tam-receptor.com)
Ethyl 2-((2 3 bis(nitrooxy)propyl)disulfanyl)benzoate (GT-094) a novel nitro-NSAID induced ROS and decreased mitochondrial membrane potential (MMP) in SW480 and RKO colon cancer cells and treatment with antioxidants GSH and DTT inhibited ROS generation prevented the loss of MMP and reversed the effects on downregulation of Sp1 Sp3 and Sp4 proteins and Sp dependent genes [42]. (tam-receptor.com)
Burden tests were performed for protein-truncating and rare missense variants in 15,616 and 18,601 genes, respectively. (cdc.gov)
Associations between protein-truncating variants and breast cancer were identified for the following six genes at exome-wide significance (P? (cdc.gov)

While the cyclin-dependent kinase inhibitor AT7519 induces neutrophil apoptosis to promote inflammation resolution in preclinical models of lung inflammation, its potential efficacy in ARDS has not been examined. (bmj.com)
AT7519 was able to override this phenotype to induce apoptosis in ARDS neutrophils with reduced expression of the pro-survival protein Mcl-1. (bmj.com)
We demonstrate the first pharmacological compound to induce neutrophil apoptosis in sepsis-related ARDS, highlighting cyclin-dependent kinase inhibitors as potential novel therapeutic agents. (bmj.com)
In this model, the senescence-sensitive promoter from the cyclin-dependent kinase inhibitor 2A ( Cdkn2a ) gene, also known as p16 INK4a , drives expression of 3MR, a fusion protein that is composed of luciferase and red fluorescent protein (RFP) reporters and herpes simplex virus-1 thymidine kinase, which converts ganciclovir (GCV) into an apoptosis inducer. (jax.org)
Diminution of p53 by RNA interference induced necrosis instead of apoptosis in A549 cells following terpinen-4-ol treatment, indicating that terpinen-4-ol-elicited apoptosis is p53-dependent. (hindawi.com)
Recent reports have indicated that terpinen-4-ol exerts its antitumor effects by triggering caspase-dependent apoptosis in human melanoma cells or by inducing necrotic cell death and cell-cycle arrest in mouse mesothelioma and melanoma cell lines without affecting normal cells [ 14 , 15 ]. (hindawi.com)
Our results indicated that terpinen-4-ol induced apoptosis through a mitochondria-mediated pathway in NSCLC cells and that the apoptosis elicited by terpinen-4-ol was p53 dependent. (hindawi.com)
It was identified that propofol inhibited the viability and invasion, but promoted apoptosis of HGC‑27 and AGS cells in a dose‑dependent manner. (spandidos-publications.com)
Hematopoietic progenitor kinase 1 (HPK1) regulates stress responses, proliferation, and apoptosis in hematopoietic cells. (aacrjournals.org)
Despite a strong trend to correlation, (p = 0.15), p21(WAF1/CIP1) expression did not correlate significantly with radiation-induced apoptosis, which suggested that p21(WAF1/CIP1) influenced tumor radioresponse by mechanisms beyond that of apoptosis induction. (elsevierpure.com)
Survivin is an apoptosis inhibitor that is associated with tumor proliferation and invasion. (springeropen.com)
Survivin belongs to the family of inhibitors of apoptosis proteins (IAPs) that plays a pivotal role in mitotic progression, apoptosis inhibition, angiogenesis, progression of cancer, and invasion [ 5 ]. (springeropen.com)
To understand the antitumor activity of deferasirox, we investigated its effect on the expression of molecules that play key roles in metastasis, cell cycle control, and apoptosis. (birmingham.ac.uk)
Moreover, this agent increased the expression of apoptosis markers, including cleaved caspase-3 and cleaved poly(ADP-ribose) polymerase 1. (birmingham.ac.uk)
Western blotting was performed for detecting changes of apoptosis-associated proteins, cell cycle regulatory proteins and state of activation of Akt, mTOR, p70S6K, 4E-BP1, and p38-MAPK. (besjournal.com)
Results Resveratrol inhibited the proliferation and induced apoptosis and autophagy in T-ALL cells in a dose and time-dependent manner. (besjournal.com)
In this study, we used histological assay (TUNEL staining) for determination of apoptosis and an LC-MS/MS system to investigate the proteins involved in ADNF-9 neuroprotection. (biomedcentral.com)
We hypothesized that ADNF-9 administered alongside prenatal alcohol exposure can prevent alcohol-induced growth deficit and apoptosis through several key proteins that are involved in fetal brain development. (biomedcentral.com)
Lonafarnib is also able to induce apoptosis via a CHOP-dependent upregulation of the DR-5-expression and a following caspase-8-activation. (uni-marburg.de)
Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. (biossusa.com)
LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. (biossusa.com)

Cyclin-dependent kinase 4 (CDK4) and retinoblastoma protein (RB) are both important cell-cycle regulators that function in different scenarios. (bvsalud.org)
Treatment of breast cancer cell lines with the combination of palbociclib and antiestrogens leads to decreased retinoblastoma protein (Rb) phosphorylation resulting in reduced E2F expression and signaling and increased growth arrest compared to treatment with each drug alone. (shu.edu)

The interactions of cyclins, cyclin-dependent kinases (CDKs), and their inhibitors regulate cell cycle progression. (springeropen.com)
Upstream inhibitors, including members of the Ink4 and Cip/Kip families, inhibit the mitogenic action of CDKs. (shu.edu)

Initial observations indicate that CCN5 induces expression of P16INK4A and P19ARF, resulting in cell cycle arrest of the tumor cells. (ku.edu)
A rise in the levels of the p53 protein induces the expression of p21 cyclin-dependent kinase inhibitor. (reactome.org)
Ectopic expression of PtaRHE1, encoding a poplar RING-H2 protein with E3 ligase activity, alters plant development and induces defence related response in tobacco. (helsinki.fi)
Active TGFβ induces the heteromeric complex formation of two single transmembrane serine/threonine kinase family receptors, i.e. (encyclopedia.pub)
Polypyrimidine tract-binding protein induces p19(ink4d) expression and inhibits the proliferation of h1299 cells. (biomedcentral.com)

Purpose: The study investigated whether basal, constitutive levels of p21(WAF1/CIP1) protein in murine carcinoma are related to in vivo tumor radioresponse. (elsevierpure.com)
The study is based on recent observations demonstrating that in vitro cancer cell lines are resistant to cytotoxic drugs when they express high basal levels of p21(WAF1/CIP1) protein, and that the loss of the p21 gene in the HCT116 human colorectal cancer cell line results in increased radioresponse of xenografts derived from that cell line. (elsevierpure.com)
Methods and Materials: Protein levels of p21(WAF1/CIP1), p53, bax, and bcl-2 were determined in 8 carcinomas (3 mammary carcinomas designated MCa-4, MCa-29, and MCa-35, 2 squamous cell carcinomas designated SCC-IV and SCC-VII, ovarian adenocarcinoma OCa-I, hepatocarcinoma HCa-I, and adenosquamous carcinoma ACa SG) syngeneic to C3Hf/Kam mice using Western blot analysis. (elsevierpure.com)
Results: Protein levels of these oncogenes varied among tumors, with p21(WAF1/CIP1) showing the greates variation: its mean densitometric value ranged from 1 to 19. (elsevierpure.com)
Tumor radioresponse correlated significantly (R = 0.77, p = 0.02) only with the magnitude of p21(WAF1/CIP1) expression: tumors with high levels of p21(WAF1/CIP1) were less radiocurable than those with lower levels. (elsevierpure.com)
The pretreatment assessment of p21(WAF1/CIP1) protein could serve as a useful predictor of radiotherapy outcome and may assist in selecting an effective treatment modality. (elsevierpure.com)
We demonstrated that deferasirox increased expression of the metastasis suppressor protein N-myc downstream-regulated gene 1 and upregulated the cyclin-dependent kinase inhibitor p21(CIP1/WAF1) while decreasing cyclin D1 levels. (birmingham.ac.uk)
Mechanistically, benzofuran derivatives enhanced the cyclin-dependent kinase inhibitor p21Cip/WAF1 expression at both mRNA and protein levels and this occurs transcriptionally in an Sp1-dependent manner. (unical.it)
When the cells were irradiated with 10 J/m2 of u.v., there was a substantial increase in expression of Waf1/ Cip1 and mdm2. (ox.ac.uk)
However, little increase in Waf1/Cip1 and mdm2 expression was observed in T22 and NIH3T3 cells 8 or 9 h after exposure to 50 J/m2 of u.v., although the p53 protein level accumulated to its highest level under these conditions. (ox.ac.uk)
Interestingly, a significant increase in Waf1/Cip1 expression was seen 24 h after irradiation in NIH3T3 cells, indicating that the inhibition of p53 transcriptional activity is reversible. (ox.ac.uk)

Cdk4-mediated phosphorylation of Rb protein is inhibited by p16, a product of a possible tumor suppressor gene. (nih.gov)
Activation of the ATM kinase by ionizing radiation and phosphorylation of p53. (reactome.org)
and (v) pRb phosphorylation and cyclin D1 expression are reduced in K10-transfected cells and increased in K16-transfected cells. (exposed-skin-care.net)
Here DARPP-32 phosphorylation by protein kinase A (PKA), DARPP-32 into a powerful protein phosphatase 1 (PP1) inhibitor. (pp1a.com)
DARPP-32 can itself inhibit the following PKA DARPP-32 phosphorylation by cyclin-dependent kinase 5 (Cdk5). (pp1a.com)
Mechanistic investigations revealed reduce GR-Ser211 phosphorylation following PP1α silencing and provide the first indication of the involvement of glycogen synthase kinase 3 (GSK-3). (pp1a.com)
During early G1 phase, mitogenic signals trigger activation of the CDK4/6-cyclin D complex, which partially deactivates Rb by phosphorylation. (shu.edu)

Mammary-specific expression of CCN5 in HER2 overexpressing mice delays tumor progression significantly and reduces the tumor burden. (ku.edu)
The detection of HPV DNA alone, in the absence of evidence for viral gene expression, is not unequivocal molecular evidence that HPV infection either causes or promotes malignant progression in a lesion. (biomedcentral.com)
We found that loss of HPK1 protein expression correlated significantly with the progression of pancreatic intraepithelial neoplasias ( P = 0.001) and development of invasive PDA. (aacrjournals.org)
Both proteins may play a role in UC progression and can have a value as prognostic and/or diagnostic markers of UC, as well as targeted therapies. (springeropen.com)
Correspondingly, most cancer signaling pathways seem to converge on one or more TFs, termed "master regulators" (MRs) [ 4 ], which direct tumor development, progression, and metastasis through hierarchical control of gene expression patterns. (aging-us.com)
cytoplasmic and nuclear high PP1 expression was associated with a shorter overall patient survival and expression of cytoplasmic PP1 high with progression-free survival shorter (P = 0.005, 0.033, and 0.037, respectively). (pp1a.com)
Cdk5 high expression was associated with progression-free survival shorter (P = 0.006). (pp1a.com)
In a Phase 3 Paloma-III study of Pfizer's CDK4/6 inhibitor ( Ibrance - palbociclib) and an estrogen receptor antagonist ( Faslodex - fulvestrant), patients receiving the combination had significantly prolonged progression-free survival than women with breast cancer who received Faslodex, alone. (shu.edu)
Normally, cyclin interacts with cyclin-dependent kinase (CDK) to form a cyclin-CDK complex, which promotes cell cycle progression, whereas cyclin-dependent kinase inhibitor (CDKI) molecules inhibit the formation of cyclin-CDK complex, arresting cell cycle. (biomedcentral.com)
Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. (biossusa.com)
Using semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR) and western blots experiments, we compared changes in ETS-1 and ETS-2 expression, their protein levels, and the regulation of some of their target gene expressions at different stages of the ocular tumoral progression in the transgenic mouse model, Tyrp-1-TAg, with those in normal eyes from control mice of the same age. (molvis.org)
RNA interference (RNAi) studies carried out in pancreatic malignancy cells shows that Sp1 Sp3 and Sp4 proteins are involved in VEGF VEGFR1 and VEGFR2 manifestation and knockdown of Sp1 Sp3 and Sp4 by RNAi also affected pancreatic malignancy cell growth and cell cycle progression with a decreased percentage of cells in G2/M and S and improved percentage of cells in G0/G1 phase. (tam-receptor.com)
p53, mdm-2, p21, and mib-1 expression were not significantly associated with response to chemotherapy, time to progression, or overall survival in the whole patient population or in the docetaxel group. (lu.se)

Cyclin-dependent kinase inhibitor p27Kip1 expression and interaction with other cell cycle-associated proteins in mammary carcinoma. (ox.ac.uk)
This leads to stabilization and nuclear localization of FOXO3A resulting in transcriptional activation of the cyclin-dependent kinase inhibitor P27KIP1. (ku.edu)
However, proliferation of cardiomyocytes induced by nuclear-targeted cyclin D1 (D1NLS) stops after one or two rounds of cell cycles due in part to accumulation of p27Kip1, an inhibitor of cyclin-dependent kinase (CDK). (elsevierpure.com)
Thus, expression of S-phase kinase-associated protein 2 (Skp2), a negative regulator of p27Kip1, significantly enhances the effect of D1NLS and CDK4 on cardiomyocyte proliferation in vitro. (elsevierpure.com)

FAK (focal adhesion kinase), ERK (extracellular signal-regulated kinase), and STAT3 (signal transducer and activator of transcription 3) were activated in senescent ECFCs with Panx1 knockdown, in which the intracellular calcium level was reduced, and the activation was inhibited by supplemented calcium. (bvsalud.org)
Furthermore, we found that p19 INK4d modulates GATA1 protein levels through a novel pathway, the phosphatidylethanolamine-binding protein 1 (PEBP1)-phosphorylated extracellular signal-regulated kinase ( p ERK)-heat shock 70 kDa protein (HSP70)-GATA1 pathway [ 3 ]. (biomedcentral.com)

p27, cyclin D1, and retinoblastoma (Rb) protein have been demonstrated using immunohistochemistry in 189 cases of primary breast carcinoma with long-term follow-up. (ox.ac.uk)
We examined the expression of p16 and Rb protein by means of immunohistochemistry in 61 non-small cell lung cancers and have demonstrated an inverse relationship between the expression of p16 and Rb protein: 28/30 specimens that did not stain for p16 stained for Rb and 21/31 p16-positive specimens did not stain for Rb. (nih.gov)
Using immunohistochemistry, expression of DARPP-32, PP1 and Cdk5 is determined in a large cohort of primary tumors from ovarian cancer patients (n = 428, 445 and 434 respectively) to evaluate the relationship between clinical outcome and clinicopathological criteria. (pp1a.com)
This is the first study describing the production and distribution of ETS-1 and ETS-2 mRNAs and proteins using in situ hybridization and immunohistochemistry in murine ocular tissue sections of normal control eyes and tumoral eyes from mice of the same age. (molvis.org)
3. Translational research - to identify predictive biomarkers for treatment decision - by immunohistochemistry, gene expression or sequencing. (lu.se)

Cyclin-dependent kinase inhibitor 1C (p57, Kip2), also known as CDKN1C, is a protein which in humans is encoded by the CDKN1C imprinted gene. (wikipedia.org)

MicroRNA (miRNA) molecules, a series of single-stranded non-coding RNA chains measuring 20-25 nucleotides in length, regulate gene expression at the transcriptional level by complementary pairing with target gene mRNA ( 5 ). (spandidos-publications.com)
Over the last two decades, studies based on genome-wide gene expression and functional profiling have revealed the great diversity of transcriptional alterations occurring in liver carcinogenesis. (aging-us.com)
This study found an increase in excess GR transcriptional activity of protein phosphatase 1 alpha (PP1α) in HEK-293 cells and a decrease in the expression levels of GR-responsive gene knockdown following PP1α model A549 cells endogenously . (pp1a.com)
Recent advances in understanding the role that epigenetics plays in cancer pathogenesis and understanding the mechanisms through which these processes regulate gene expression have stimulated considerable interest in developing clinically viable antineoplastic agents that target enzymatic components of transcriptional regulatory complexes responsible for the establishment of pathologic epigenetic modifications that lead to deregulated gene expression in cancer. (aacrjournals.org)
Discordance between accumulated p53 protein level and its transcriptional activity in response to u.v. radiation. (ox.ac.uk)
Discordance between the transcriptional activity of p53 and its protein level was further studied using a cell line expressing the p53 reporter plasmid RGC delta fosLacZ. (ox.ac.uk)
Transcription factors (TFs) are critical for B-cell differentiation, affecting gene expression both by repres- sion and transcriptional activation. (lu.se)

Elevation of the Bax/Bcl-2 ratio and a decrease in IAP family proteins XIAP and survivin were also observed following terpinen-4-ol treatment. (hindawi.com)
The overexpression of miRNA‑375 significantly increased caspase‑3 and caspase‑9 activities, induced B‑cell lymphoma 2 (Bcl‑2)/Bcl‑2‑associated X protein, tumor protein 53 and cyclin‑dependent kinase inhibitor 1 protein expression and suppressed cyclin D1 and survivin protein expression in HPV‑18(+) cervical cancer cells. (spandidos-publications.com)
We aimed to investigate the expression of survivin and P27 in UC of urinary bladder and correlate their expressions with histopathological parameters in an attempt at studying the possibility of their use as targeted therapies. (springeropen.com)
By logistic regression test, survivin expression can be a predictive risk factor associated with solid pattern and high-grade UC, while P27 expression can be a predictive risk factor associated low-grade UC. (springeropen.com)
High survivin and low P27 expression scores were associated with the studied prognostic factors of UC. (springeropen.com)
In the present study, we investigated the significance of survivin and P27 expressions in UC of the urinary bladder, by correlating their expression with clinicopathological features, including tumor pattern (papillary or solid), grade of differentiation, and stage of invasion (non-muscle or muscle-invasive). (springeropen.com)

ETS-1 and ETS-2 mRNA and protein levels were much higher in the ocular tissues of Tyrp-1-TAg mice than in control ocular tissues from wild-type mice. (molvis.org)

Cyclin-dependent kinase inhibitor 1C is a tight-binding inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. (wikipedia.org)
In GC, Yang et al ( 7 ) reported that propofol suppressed the proliferation of SGC-7901 and MGC-803 cells by promoting inhibitor of growth 3 ( 7 ). (spandidos-publications.com)
We found that K10 expression leads to the inhibition of cell proliferation while K16 appears to facilitate this process. (exposed-skin-care.net)
P27 is a cyclin-dependent kinase inhibitor that negatively regulates cell proliferation. (springeropen.com)
PTEN encodes a protein kinase of the same name and functions as a tumor suppressor through regulation of cell proliferation. (medscape.com)
Cyclin D1 and CDK4/6 are downstream of signaling pathways which lead to cellular proliferation. (shu.edu)
Conclusion: Expression of Skp2 enhanced the effect of D1NLS and CDK4 on the proliferation of cardiomyocytes and further contributed to improved post-ischaemic cardiac function. (elsevierpure.com)
Similarly, depletion in the STRIPAK component STRIP1 affects activation of GCKIII kinases and cell cycle disruption through elevated expression of cyclin dependent kinase inhibitors p21 and p27, enhanced levels of which lead to a protective effect from therapeutic treatments and increased proliferation. (lu.se)

[ 2 ] Options for second-line therapy in patients with relapsed/refractory disease include chemotherapy-free regimens with biologic targeted agents such as covalent Bruton tyrosine kinase (BTK) inhibitors, lenalidomide,venetoclax, and chimeric antigen receptor (CAR) T-cell therapy. (medscape.com)

From the abstract: ' Is Ki-67 expression associated with the 21-gene recurrence score (RS) and with outcomes in patients with breast cancer with a low RS? (cdc.gov)

Complete hydatidiform moles consist only of paternal DNA, and thus the cells lack p57 expression as the gene is paternally imprinted (silenced). (wikipedia.org)
Next, we have shown that CCN5 protein can induce expression of estrogen receptor-α (ER-α) in mammary epithelial cells. (ku.edu)
We found that mammary epithelium-specific overexpression of CCN5 in transgenic mice leads to an increase in ER-α expression and that this impact of CCN5 is not restricted to the normal cells. (ku.edu)
CCN5 treatment leads to an expression of functional ER-α in the TNBC cells, both in-vitro and in xenograft models, and sensitizes these cells to tamoxifen, commonly used for endocrine therapies. (ku.edu)
Lack of ER-α expression in TNBC cells or loss of ER-α activation after endocrine treatment of luminal cancers makes these breast cancer cells resistant to tamoxifen and other endocrine therapies. (ku.edu)
Furthermore, reverse transcription‑quantitative PCR was used to examine the expression levels of circular RNA‑PVT1 (circ‑PVT1), micoRNA‑195‑5p (miR‑195‑5p) and ETS1 in GC tissues and cells. (spandidos-publications.com)
Propofol could inhibit Bcl‑2 and MMP9 expression, and increase P21 expression in GC cells. (spandidos-publications.com)
The expression levels of circ‑PVT1 and ETS1 were increased in GC tissues and cells, and miR‑195‑5p was decreased. (spandidos-publications.com)
Additionally, the treatment with propofol could lead to decreased circ‑PVT1 expression in GC cells. (spandidos-publications.com)
Additionally, the effects of transfection with circ‑PVT1 small interfering RNA (si‑circ‑PVT1) on HGC‑27 and AGS cells could be reversed by treatment with miR‑195‑5p inhibitor. (spandidos-publications.com)
The overexpression of miRNA‑375 significantly suppressed the levels of protein expression of ubiquitin‑protein ligase E3A (UBE3A) and Insulin‑like growth factor‑1 receptor (IGF‑1R) in HPV‑18(+) cervical cancer cells. (spandidos-publications.com)
UBE3A is associated with cervical cancer and may combine with the E6 proto-oncogene encoded by HPV16 within cervical cancer cells to form the E6/E6-AP protein complex through the ubiquitin proteasome pathway ( 4 ). (spandidos-publications.com)
Previous studies have identified that UBE3A exhibits abnormal expression in numerous tumor cells, including prostate, cervical and breast cancer ( 6 , 7 ). (spandidos-publications.com)
Similarly, HPK1 protein was not expressed in any of eight PDA cell lines examined but was expressed in immortalized human pancreatic duct epithelial (HPDE) cells. (aacrjournals.org)
Treatment of Panc-1 cells with a proteasome inhibitor, MG132, increased the HPK1 protein levels in a dose-dependent manner, suggesting that alteration in proteasome activity contributes to the loss of HPK1 protein expression in pancreatic cancer. (aacrjournals.org)
Like the endogenous HPK1, both wild-type HPK1 and its kinase-dead mutant, HPK1-M46, overexpressed in Panc-1 cells, were also targeted by proteasome-mediated degradation. (aacrjournals.org)
Furthermore, restoring wild-type HPK1 protein in PDA cells led to the increase in p21 and p27 protein expression and cell cycle arrest. (aacrjournals.org)
Keratins are a large family of proteins which form the intermediate filament (IF) cytoskeleton of epithelial cells and their appendages hairs and nails (reviewed in references 8 and 15). (exposed-skin-care.net)
Immunopositivity (number of positive cases) and expression score (percentage of positive urothelial cells) were evaluated. (springeropen.com)
Immunohistochemical analysis showed that Skp2 protein was expressed predominantly in carcinoma cells. (elsevierpure.com)
Western blot analysis showed that Skp2-transfected cells expressed lower levels of p27 protein than the control cells. (elsevierpure.com)
We observed that benzofuran compounds bearing a phenyl or tert-butyl substituent α to the methoxycarbonyl group significantly inhibited anchorage-dependent and -independent cell growth, and induced G0/G1 cell cycle arrest in human estrogen receptor alpha positive (MCF-7 and T47D) and in triple negative MDA-MB-231 breast cancer cells, without affecting growth of MCF-10A normal breast epithelial cells. (unical.it)
Inhibition of notch signaling by gamma secretase inhibitor engages the rb pathway and elicits cell cycle exit in t-cell acute lymphoblastic leukemia cells. (biomedcentral.com)
The protein expression profile, morphological characteristics, and electrophysiological properties of SGN-like cells in otic organoids were similar to those of primary SGNs. (elsevierpure.com)
Furthermore, a cyclin-dependent kinase-2 inhibitor suppressed the toxic actions of cisplatin on SGN-like cells in otic organoids. (elsevierpure.com)
In this compiled work, we elaborate on the effect of protein expression alterations and their resulting effect on recurrence capability of cells. (lu.se)

An INK4 cyclin-dependent kinase inhibitor containing five ANKYRIN-LIKE REPEATS. (childrensmercy.org)

Western blotting revealed that resveratrol significantly decreased the expression of antiapoptotic proteins (Mcl-1 and Bcl-2) and increased the expression of proapoptotic proteins (Bax, Bim, and Bad), and induced cleaved-caspase-3 in a time-dependent manner. (besjournal.com)
Diverse models show that Flavoprirol inhbitis the TNF-mediated NF-κB-activation and the AP-1-activation, suppresses the expression of a multiplicity of antiapoptotic proteins and the TNF-induced AKT-activation. (uni-marburg.de)

In response to DNA double strand breaks, serine at position 15 of the TP53 (p53) tumor suppressor protein is rapidly phosphorylated by the ATM kinase. (reactome.org)

Among them, the sirtuin, AMP-activated protein kinase, mammalian target of rapamycin, p53, and insulin/insulin-like growth factor-1 signaling pathways are most widely studied. (frontiersin.org)
What pathways are this gene/protein implicaed in? (cancerindex.org)
Over-expression of COMP leads to activation of Notch which affects both Wnt/β-catenin and AKT pathways, already affected by COMP. (lu.se)

The members from the large keratin family of cytoskeletal proteins are expressed in a carefully regulated tissue- and differentiation-specific manner. (exposed-skin-care.net)
Label-free quantitative proteomic analyses using liquid chromatography in conjunction with a tandem mass spectrometry (LC-MS/MS) system showed significant alteration of mitochondrial, cytosolic, nuclear and cytoskeletal proteins in fetal brains exposed prenatally to alcohol [ 17 ]. (biomedcentral.com)

Combined application of the CDK4 inhibitor and FRMD8 peptide leads to marked suppression of CRC cell growth. (bvsalud.org)
Therefore, using an LKCHE-containing peptide to interfere with the MDM2-RB interaction may have therapeutic value in CDK4/6 inhibitor-resistant patients. (bvsalud.org)
Compared with rats that received only D1NLS and CDK4, expression of Skp2 improved left ventricular function as measured by the maximum and minimum rates of change in left ventricular pressure, the left ventricle end-diastolic pressure, left ventricle end-diastolic volume index, and the lung/body weight ratio. (elsevierpure.com)

Activation of the CDK2-cyclin E complex results in hyperphosphorylation of Rb and fully releases E2F. (shu.edu)

E6-AP) is an important member of the ubiquitin proteasome system and a type of ubiquitin protein ligase (E3 enzyme) ( 6 ). (spandidos-publications.com)
S-phase kinase-associated protein 2 (Skp2), a member of the F-box family of substrate-recognition subunits of Skp1-Cullin-F-box ubiquitin-protein ligase complexes, is necessary for p27 ubiquitination and degradation. (elsevierpure.com)

We also demonstrated upregulation of ETS-1 and ETS-2 target expressions in Tyrp-1-TAg mice when comparing with the same target expressions in control mice. (molvis.org)

Transcription factors (TFs) drive gene expression programs that shape specific phenotypes [ 3 ], and are frequently dysregulated in cancer [ 4 ]. (aging-us.com)
p53 is a transcription factor whose expression is increased by DNA damage and blocks cell division at the G1 phase of the cell cycle to allow DNA repair. (medscape.com)
By acting as a ligand-dependent transcription factor of the glucocorticoid receptor (GR) mediates the action of glucocorticoids and regulates many physiological processes. (pp1a.com)
The identified transcription factors influence both the global and specific gene expression of the BCLs and have possible implications for diagnosis and treat- ment. (lu.se)

We have shown that in clinical material p27 expression is associated with proliferative activity and while univariate analysis shows it to be a significant indicator of prognosis, this significance is lost in multivariate analysis when traditional prognostic factors are included in the model. (ox.ac.uk)
MMP high expression groups (MMP score ≥5) had worse prognosis than low-expression groups. (cancerindex.org)
The FRMD8 promoter is hypermethylated, and low expression of FRMD8 predicts poor prognosis in CRC patients. (bvsalud.org)
Reduced expression level of p27, a cyclin-dependent kinase inhibitor, is associated with high aggressiveness and poor prognosis of various malignant tumors, including gastric carcinoma. (elsevierpure.com)

However, expression profiling has often focused on either the host or the pathogen due to limitations of methods that involve microarrays. (prelekara.sk)
p53, mdm-2, p21, and mib-1 expression were assessed by immunohistochemical methods in primary tumors derived from 134 patients who took part in a randomized multicenter trial comparing docetaxel to sequential methotrexate and 5-fluorouracil (MF) in advanced breast cancer. (lu.se)

Our results indirectly support the theory that p16 expression is negatively regulated by the functional Rb protein. (nih.gov)

Multivariable analysis revealed only HPV16 E6/E7 mRNA expression to have significant prognostic influence ( p = 0.04). (biomedcentral.com)
These data demonstrate the role of DARPP-32 and related kinase signaling as a prognostic marker in ovarian cancer clinical utility. (pp1a.com)
In the last decade, diagnostic and prognostic evaluation has been facilitated by global gene expression profiling (GEP), provid- ing a new powerful means for the classification, prediction of survival, and response to treatment of lym- phomas. (lu.se)

It encodes a cell cycle inhibitor that binds to G1 cyclin-CDK complexes. (wikipedia.org)
RB1 encodes the protein pRB and was the first tumor suppressor gene to be molecularly defined. (medscape.com)
TP53 encodes the protein p53, which is known as the "guardian of the genome. (medscape.com)

Western blotting was performed to detect the protein levels of cyclin‑dependent kinase inhibitor P21 (P21), B‑cell lymphoma‑2 (Bcl‑2), matrix metalloproteinase 9 (MMP9) and E26 oncogene homolog 1 (ETS1). (spandidos-publications.com)
In addition, numerous important cellular proteins, such as B-cell lymphoma-2 homologous antagonist/killer, Myc proto-oncogene protein, cyclin-dependent kinase inhibitor 1B, DNA replication licensing factor MCM-7, retinoblastoma 1 and Annexin A1, are degenerated through the UBE3A-mediated ubiquitin proteasome pathway ( 7 ). (spandidos-publications.com)

There was a statistically significant association between the expression of p27 and both cyclin D1 and the retinoblastoma gene product (pRb), corresponding to their close interactions in regulating the G1/S transition in the cell cycle. (ox.ac.uk)
A comprehensive understanding of host-pathogen interactions requires knowledge of the dynamics of gene expression changes in both the host and the pathogen during a time course of infection. (prelekara.sk)

The interest in p27 expression in mammary carcinoma lies in its behaviour when examined in combination with other G1 cell cycle regulators. (ox.ac.uk)
P27 is a cyclin-dependent kinase inhibitor (CKI) that controls protein synthesis during the cell cycle. (springeropen.com)
These kinases are expressed throughout the cell cycle, but are only activated upon complex formation with their corresponding cyclins. (shu.edu)
It also induced cell cycle arrest at G0/G1 phase via up regulating cyclin-dependent kinase (CDK) inhibitors p21 and p27 and down regulating cyclin A and cyclin D1. (besjournal.com)
Unexpectedly, knockdown of p19 INK4d did not affect cell cycle, and these functions caused by p19 INK4d knockdown were via decreasing levels of GATA-binding protein 1 (GATA1). (biomedcentral.com)
Aims: Cyclins and other cell-cycle regulators have been used in several studies to regenerate cardiomyocytes in ischaemic heart failure. (elsevierpure.com)

Finally, we discuss the effects of CCN5 expression on yet another aggressive breast cancer subtype, characterized by HER2 overexpression. (ku.edu)
CA is caused by the epithelial-associated proteins found in the genomes of each subtype ( 2 , 3 ). (spandidos-publications.com)

The increased IGF-1 in Panx1-knockdown ECFCs was abrogated, respectively, by inhibitors of FAK (PF562271), ERK (U0126), and STAT3 (NSC74859) and supplemented calcium. (bvsalud.org)
This was accompanied by increased manifestation of cyclin-dependent kinase inhibitor p27 with Sp3 knockdown [54-56]. (tam-receptor.com)

The directed expression of SV40 T antigen has led to the development of several important transgenic models with spontaneous epithelial tumor formation. (molvis.org)

The 78 kDa glucose regulated protein/BiP (GRP78) belongs to the family of ~70 kDa heat shockproteins (HSP 70). (exposed-skin-care.net)

In univariate analysis, low p27 expression was associated with a reduced relapse-free and overall survival. (ox.ac.uk)
When the combined expression of p27 and cyclin D1 was related to survival, patients with high levels of p27, regardless of their cyclin D1 status, did well, whilst those with low p27 had a poor outcome. (ox.ac.uk)

its gene expression is regulated through the activity of glucocorticoid receptors (GRs) through chromatin remodelling mediated by SWI/SNF. (wikipedia.org)
A derived peptide from activity-dependent neurotrophic factor (ADNF-9) has been shown to be neuroprotective in the fetal alcohol exposure model. (biomedcentral.com)
An important mechanism by which the expression, localization, and activity of TGFβ signaling components is controlled is by post-translational modification (PTMs). (encyclopedia.pub)

In this study, we examined the expressions of p33ING1, p14ARF, MDM2 and p21WAF1 mRNA in 25 advanced colorectal cancers by quantitative RT-PCR method, and compared the expression levels of p33ING1, p14ARF, p21WAF1 and MDM2 in relation to p53 status in the tumors. (nih.gov)
Only one case with wild-type p53 gene showed normal level expression of p53 regulatory-factors (p33ING1, p14ARF, and MDM2). (nih.gov)

Significant upregulation of several important proteins involved in brain development were found in the ALC/ADNF-9 group as compared to the ALC group. (biomedcentral.com)

Anatomy13

Organisms9

Diseases5

Chemicals and Drugs163

Analytical, Diagnostic and Therapeutic Techniques and Equipment13

Phenomena and Processes49

Disciplines and Occupations1

Information Science3

Genes15

Apoptosis21

Retinoblastoma Protein2

CDKs2

Induces5

WAF111

Phosphorylation7

Progression13

P27Kip14

Extracellular signal2

Immunohistochemistry5

Humans1

Transcriptional7

Survivin6

MRNA and protein levels1

Proliferation8

Tyrosine1

Abstract1

Cells26

INK41

Antiapoptotic proteins2

Serine1

Pathways3

Cytoskeletal proteins2

CDK43

Activation1

Ubiquitin protei2

Mice1

Transcription4

Prognosis4

Methods2

Negatively1

Prognostic3

Encodes3

Lymphoma2

Interactions2

Cell cycle6

Subtype2

Knockdown2

Antigen1

Belongs to the family1

Survival2

Activity3

MDM22

Upregulation1