Protein expression expression of micrornas micro rna that regulates. Medical search. Frequent questions

MicroRNAs (miRNAs) are transcribed as ~70 nucleotide precursors and subsequently processed by the RNase-III type enzyme Dicer to give a ~22 nucleotide mature product. (wikipedia.org)
miRNAs are a family of small non-coding RNAs that emerged as significant and versatile regulators involved in regulation of immune responses. (frontiersin.org)
MicroRNAs (miRNAs) are highly conserved, single-stranded, small non-coding RNAs that have been shown to act as essential regulators of gene expression at the posttranscriptional level. (frontiersin.org)
Furthermore, the miRNAs correlated negatively with the immunohistochemical expression of NRF1 and NRF2 but positively with their respective mRNA. (hindawi.com)
MicroRNAs (miRNAs) are small noncoding RNAs that posttranscriptionally regulate gene expression by imperfect matching of mRNA [ 6 ]. (hindawi.com)
MicroRNAs (miRNAs) are small, endogenous, non-coding RNAs, which act as key post-transcriptional regulators of target-gene expression. (spandidos-publications.com)
1,000 human miRNAs have been identified that regulate ~1/3 of the coding genes in the human genome ( 9 ). (spandidos-publications.com)
In contrast, a cluster of viral microRNAs (miRNAs) called miR-BARTs is highly expressed. (duke.edu)
MiRNAs are small non-coding RNAs which regulate gene expression by binding to complementary sequences in mRNAs. (duke.edu)
Individual miRNAs are capable of regulating gene networks by interacting with multiple targets or by suppressing transcriptional controllers [ 6 , 8 ]. (researchsquare.com)
miRNAs are essential for brain development and function, with multiple miRNAs enriched in specific cell types where they regulate differentiation, structure and neurophysiological properties [ 9 , 10 ]. (researchsquare.com)
Expression of various miRNAs is dysregulated in experimental models of status epilepticus and in resected brain tissue from patients with drug-resistant temporal lobe epilepsy [ 11-13 ]. (researchsquare.com)
The aberrant expression of miRNAs is associated with the pathogenesis of sepsis. (degruyter.com)
MicroRNAs (miRNAs) are a kind of small noncoding RNAs that regulate the expression of protein through targeting the 3′UTR of messenger RNA (mRNA), resulting in mRNA degradation or suppression of translation [ 5 ]. (degruyter.com)
MicroRNAs (miRNAs) are single-stranded RNA molecules of approximately 22 nucleotides. (rupress.org)
Now running his own laboratory at Yale, Giraldez has stuck with zebrafish and investigates how miRNAs shape gene expression to orchestrate embryonic development ( 6 ). (rupress.org)
Micro RNAs (MiRNAs) are endogenous, small RNA molecules that were identified in a wide range of eukaryotes. (usda.gov)
About 30% of the human protein-coding genes are negatively regulated by miRNAs which suggests that they have a major role in regulating gene expression. (usda.gov)
We now have identified and characterized 54 miRNAs from rainbow trout somatic tissues and profiled their expression patterns. (usda.gov)
The majority of the miRNAs showed characteristic tissue-specific expression patterns suggesting potential roles in maintaining tissue identity. (usda.gov)
Recently, much attention has focused on the impact of microRNAs (miRNAs) on tumorigenesis and cancer progression. (sarcomahelp.org)
miRNAs are approximately 22-nucleotide non-coding RNAs that participate in post transcriptional gene expression regulation through mRNA degradation, translational inhibition or chromatin-based silencing mechanisms. (sarcomahelp.org)
miRNA deregulation has been identified in a variety of epithelial origin cancers, where changes in specific miRNAs expression possibly contribute to tumor growth, progression, metastasis, and chemoresistance. (sarcomahelp.org)
Pharmacologic manipulation of microRNA expression has been undertaken in the form of in vivo miRNA delivery of downregulated miRNAs and "antagomirs" targeting upregulated miRNAs. (sarcomahelp.org)
Comparable to a 'Trojan horse', it can be assumed that a whole series of metabolic events will be triggered as a positive feedback loop in any given gene network that is regulated by miRNAs. (ldsfreedomforum.com)
It is also known that most of the miRNAs that operate in the nucleus will simultaneously regulate transcript stability in the cytoplasm and vice versa, resulting in a highly integrated mechanism. (ldsfreedomforum.com)
Our recent study showed that control of GNA13 expression by specific microRNAs (miRNAs or miRs) is important for prostate cancer cell invasion. (biomedcentral.com)
To determine the probable miRNAs regulating GNA13, online miRNA target prediction tool Targetscan and Luciferase assays with GNA13-3′-UTR were used. (biomedcentral.com)
Effect of miRNAs on GNA13 mRNA, protein and invasion was studied using RT-PCR, western blotting and in vitro Boyden chamber assay respectively. (biomedcentral.com)
Expression analysis of miRNAs predicted to bind the 3′-UTR of GNA13 revealed that miR-31 exhibited an inverse correlation to GNA13 protein expression in breast cancer cells. (biomedcentral.com)
MicroRNAs (miRNAs) are short (21-24 nucleotides) sequences of RNA that reduce gene expression by promoting the breakdown of messenger RNAs (mRNAs) and by repressing translation of mRNAs into proteins. (freethoughtblogs.com)
We show that, similar to metazoan steady-state systems, endogenous miRNAs in Chlamydomonas can regulate gene expression both by destabilization of the mRNA and by translational repression. (freethoughtblogs.com)
This thesis focuses on microRNAs (miRNAs), which modulate gene expression in healthy and tumour cells. (lu.se)
MicroRNAs (miRNAs) are an integral part of the post-transcriptional machinery of gene expression and have been implicated in the carcinogenic cascade. (cdc.gov)
Six miRNAs had concordant expression in both tumors and breast cancer patient blood samples compared with the normal control samples. (cdc.gov)
Twelve miRNAs showed concordant expression in tumors vs. normal breast tissues and patient survival (n = 1093), with seven as potential tumor suppressors and five as potential oncomiRs. (cdc.gov)
From experimentally validated target genes of these 86 miRNAs, pan-sensitive and pan-resistant genes with concordant mRNA and protein expression associated with in-vitro drug response to 19 NCCN-recommended breast cancer drugs were selected. (cdc.gov)
that asthma is intensively regulated by a variety of microRNAs (miRNAs). (cdc.gov)
MiRNAs are approximately 19- to 25-nucleotide estimated about 250,000 and mortality does not appear single-stranded, noncoding RNAs that exist in both to correlate well with prevalence. (cdc.gov)
As binding of miRNAs to a target mRNA may result in an airway inflammatory disease closely correlates with either mRNA degradation or inhibition of protein immune regulation, more and more evidences suggest translation.1 More than 2000 miRNAs have been discovered in humans. (cdc.gov)
Th)1 cells.5 Meanwhile, the function and immunity of with genetic predisposition, involving multiple cells, DCs are closely regulated by miRNAs. (cdc.gov)

Small noncoding RNAs called microRNAs (miRNA) negatively regulate gene expression by sequence-specific targeting of protein-coding transcripts [ 6 ]. (researchsquare.com)
Binding of a miRNA to a complementary sequence in a target mRNA is mediated by Argonaute proteins leading to degradation of the mRNA or translational inhibition, thereby lowering protein levels in cells [ 7 ]. (researchsquare.com)
Identification of miRNA-regulated targeting genes is important for understanding their specific biological functions [ 11 ]. (degruyter.com)
Background: MicroRNAs (miRNA) are small, highly conserved, non-coding RNAs that regulate gene expression of target mRNAs through cleavage or translational inhibition. (usda.gov)
Real-time PCR was used to measure miRNA expression patterns in adult somatic tissues and unfertilized eggs. (usda.gov)
Not much is known about the expression and deregulation of miRNA in STS generally and in LPS specifically. (sarcomahelp.org)
In a recent study using a microarray approach, 27 STS of seven different histological subtypes were profiled for miRNA expression. (sarcomahelp.org)
8 The results demonstrated that miRNA expression signatures were clearly distinct among the tumor types studied, suggesting their possible role in sarcomagenesis, and their potential as diagnostic markers or even therapeutic targets. (sarcomahelp.org)
consequently, no major conclusions regarding the expression and regulation of miRNA in LPS could be made. (sarcomahelp.org)
Our studies, supported by the Liddy Shriver Sarcoma Initiative, aimed to identify unique miRNA expression profiles of WDLPS and DDLPS with the hope that such signatures could provide insights into our understanding of the molecular determinants driving these two entities. (sarcomahelp.org)
Furthermore, pumilio homolog 2 (PUM2), a RNA-binding protein, mediated the packaging of miRNA-130a into exosomes. (dovepress.com)
The miRNA functions as a guide by base-pairing with target mRNA to negatively regulate its expression. (ldsfreedomforum.com)
It means miRNA turns off protective genes in what is called down regulating (Gene Silencing) of those genes. (ldsfreedomforum.com)
An oncomir (also oncomiR) is a microRNA (miRNA) that is associated with cancer. (ldsfreedomforum.com)
It is reasonable to assume that some viral messenger RNAs will trigger changes in the host miRNA transcription profiles or stabilities and that the resulting modified miRNA clusters might favor the development of different disorders. (ldsfreedomforum.com)
The nature of imperfect binding specificity means that a single miRNA can regulate a large number of mRNA targets involved in complex cellular processes, thereby tightly controlling genetic networks during development and in response to stress ( Pocock, 2011 ). (elifesciences.org)
Here, we provide a comprehensive global analysis of the evolutionarily distant unicellular green alga Chlamydomonas reinhardtii to quantify the effects of miRNA on protein synthesis and RNA abundance. (freethoughtblogs.com)
Most of the gene mutations involved in DICER1 syndrome lead to an abnormally short Dicer protein that is unable to aid in the production of miRNA. (medlineplus.gov)

MicroRNAs are tiny ribonucleic acid (RNA) molecules (~22 nucleotides long) that recently have been found to play important roles in regulating gene expression in eukaryotic organisms, including plants and animals. (sciencedaily.com)
MicroRNA consists of short strings of about 22 nucleotides, the building blocks that make up RNA and DNA. (mit.edu)

Messenger RNA (mRNA) molecules are encoded by genes and are themselves templates for the proteins that carry the main metabolic functions in a cell. (sciencedaily.com)
The mRNA levels in a cell are fine tuned by different mechanisms, one of which is driven by microRNA molecules. (sciencedaily.com)
MicroRNAs are ~22 nucleotide long RNA molecules that provide substrate specificity to a protein complex known as the RNA-induced silencing complex. (sciencedaily.com)
Within the complex, microRNAs are thought to bind to mRNA molecules containing a complementary stretch of RNA sequence. (sciencedaily.com)
An international team led by researchers at Dartmouth and the Max Delbrück Center in Germany report in the journal Science Advances that octopuses are the first known invertebrates-creatures that lack a backbone and constitute roughly 95% of animal species-to contain a high number of gene-regulating molecules known as microRNAs. (sflorg.com)
Yet, the prevalence of microRNAs in both octopuses and vertebrates suggest a common role for the molecules in advanced cognition. (sflorg.com)
This work led to my thesis in neuroscience where I built on my study of microRNAs to research how to use the presence of these molecules in human blood to detect glioblastoma (terminal brain cancer) growth in patients. (sflorg.com)
Each sponge can bind up to six microRNA molecules, but they could be engineered to bind more. (mit.edu)
Heterotrimeric G-proteins, consisting of Gα, Gβ and Gγ subunits are the major effector molecules of GPCR signaling. (biomedcentral.com)
What we used to call 'junk DNA' has proved to be made up of areas that produce small molecules known as non-coding RNAs. (lu.se)
Around ten years ago, it was discovered that these areas code for a number of small RNA molecules that are now called non-coding RNAs because they don't code for proteins. (lu.se)
Sofia Elmroth and her research team primarily study microRNAs, which are molecules that influence protein levels in the cells of the body indirectly by binding to messenger RNA. (lu.se)
There are many different types of microRNAs involved in the regulation of different proteins and of other RNA molecules. (lu.se)
It has proved difficult to develop drugs that act directly on epigenetic changes in DNA, but now it is possible instead to intervene later in the cell metabolism and regulate the level of specific microRNA molecules. (lu.se)

mRNAs corresponding to several regulatory genes that mediate auxin responses contain short stretches of sequence that are complementary to microRNAs, and therefore have been considered potential targets of microRNA-mediated regulation. (sciencedaily.com)
The plants showed increased accumulation of ARF17 mRNA and altered levels of mRNAs corresponding to several genes that may be regulated by ARF17. (sciencedaily.com)
Lin28/LIN-28 also bind and regulate many mRNAs in diverse cell types. (stanford.edu)
microRNAs are small non-coding RNA fragments that bind to target mRNAs to regulate their stability and/or translation. (metu.edu.tr)
Although more and animals and plants and regulate gene/protein expression more progressions have been made about the through direct complementarity between their 5' region pathogenesis of asthma in recent years, the increasing and the 3' untranslated region of target mRNAs. (cdc.gov)

In these conditions, they regulate the tumor suppressor gene CDON. (wikipedia.org)
In addition, the expression of miR-181a was found to be inversely correlated with tumor grading while miR-181b was uniformly downregulated in gliomas with different grades of malignancy. (wikipedia.org)
Recently, an increasing number of studies have revealed that microRNA-497 (miR-497) levels are decreased in tumors, and that it functions as a tumor suppressor in a number of types of human cancer, including colorectal, gastric, cervical and breast cancers, adrenocortical carcinoma and melanoma ( 12 - 20 ). (spandidos-publications.com)
We aimed to determine the signaling pathway regulating cell metabolism and the mechanism related to the regulation of human tumor-associated macrophages (TAMs) in gastric cancer (GC). (cancerindex.org)
Tumor-infiltrated macrophages were isolated from human GC tissues using magnetic beads, gene transcription was determined by real-time PCR, protein expression was monitored using western blots, metabolites were determined using HPLC, and transcriptional regulation was analyzed by the luciferase-based reporter gene system. (cancerindex.org)
The new technique could shed more light on microRNA's role in tumor development: Earlier studies have shown that a type of microRNA known as let-7 inhibits a cancer-inducing gene called RAS. (mit.edu)
Cancer-associated fibroblasts (CAFs) have been demonstrated to be essential stromal components in the tumor microenvironment, regulating cancer cell growth, migration, invasion, and chemo-response through various mechanisms. (dovepress.com)
Efforts to understand the genes and signaling pathways that promote tumor invasion and metastasis have revealed that several G-protein coupled receptors (GPCRs) and their respective ligands function as metastatic drivers in breast as well other cancer types [ 2 - 6 ]. (biomedcentral.com)

Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)

Finally, IGF‑1R protein, phosphoinositide 3‑kinase/Akt signaling pathway‑associated proteins and cyclin pathway‑associated proteins were differentially expressed between miR‑497‑overexpressing cells and miR-497-silenced cells. (spandidos-publications.com)
Utilizing a Wnt signaling FLuc reporter TOPflash which measures the Wnt signaling activation, I confirmed that expression of many miR-BARTs that target Wnt signaling inhibitors can indeed upregulate the Wnt signaling pathway. (duke.edu)
Transforming growth factor-β (TGF-β) signaling pathway is a multifunctional cytokine and plays important roles in regulating cell proliferation, cell cycle, differentiation, migration, and apoptosis in a broad spectrum of tissues [ 12 ]. (degruyter.com)

They target and modulate protein expression by inhibiting translation and / or inducing degradation of target messenger RNAs. (wikipedia.org)
Autophagy is a degradation system that involves sequestration of cytoplasmic proteins and organelles by double-layered membranes that form vesicles called autophagosomes. (elifesciences.org)
Fusion of autophagosomes with lysosomes results in degradation of their contents and thereby removes toxic proteins and damaged organelles from cells to maintain homeostasis. (elifesciences.org)

Malfunctions in microRNA have been linked with cancer, but very few direct relationships have been established between specific microRNAs and the genes they regulate. (mit.edu)
(12 ). However, the versatility of the system allows the use of any microRNA of choice, including neuron-specific microRNAs ( 14 ). (lu.se)

Following recognition of TLR ligands, TLRs elicit innate immunity by activating multiple intracellular signaling cascades including variable adaptor proteins and transcriptional factors. (frontiersin.org)
Together, these microRNAs cooperatively regulate expression of the E-cadherin transcriptional repressors ZEB1 (also known as deltaEF1) and SIP1 (also known as ZEB2), factors previously implicated in EMT and tumour metastasis. (nih.gov)
MicroRNAs perform important roles in the post-transcriptional regulation of gene expression. (researchsquare.com)
A number of post-transcriptional mechanisms fine-tune the gene expression landscape. (researchsquare.com)
These data provide strong evidence that GNA13 expression in breast cancer cells is regulated by post-transcriptional mechanisms involving miR-31. (biomedcentral.com)

In this article the researchers describe their identification of important individual microRNAs and relevant genes they regulate to control regulatory T cell development. (rvc.ac.uk)

In humans, this microRNA is involved in the mechanisms of immunity, and in many different cancers (see below) it was found to be expressed at a particularly low level. (wikipedia.org)
however, the expression, function and underlying mechanisms of miR‑497 in HCC remain unclear. (spandidos-publications.com)
Due to the central role of autophagy in the removal of aggregation-prone proteins, a better understanding of mechanisms controlling autophagy is essential for the identification of novel therapeutic opportunities for multiple disease states. (elifesciences.org)
Although they are structurally similar, plant and animal microRNAs repress gene expression through very different mechanisms. (freethoughtblogs.com)
OBJECTIVE To investigate the expression of differential expressed microRNAs and the predicted target gene in Lipopolysaccharides( LPS)-induced innate immune response of THP-1 macrophages at different time points( 4,8,12,24,48 h).And to explore the host immuno-inflammatory response regulatory mechanisms of bone grafting at early stage. (cnki.net)

It has been shown that miR-181 targets the homeobox protein Hox-A11 and participates in establishing muscle tissue downregulating it (a repressor of the differentiation process in mammalians and lower organisms). (wikipedia.org)
Mammalian Lin28 and C. elegans LIN-28 have been shown to inhibit biogenesis of the conserved cellular differentiation-promoting microRNA let-7 by directly binding to unprocessed let-7 transcripts. (stanford.edu)
Through this role in regulating the activity (expression) of genes, the Dicer protein is involved in many processes, including cell growth and division (proliferation) and the maturation of cells to take on specialized functions (differentiation). (medlineplus.gov)
MicroRNAs not only participate in determining DCs phenotype and then naive T lymphocyte differentiation, but also participate in the regulation of airway inflammation and airway remodeling in asthma. (cdc.gov)

Sequencing as well as functional studies using antisense oligonucleotides, indicate important roles for microRNAs during the development of epilepsy through targeting transcripts involved in neuronal structure, gliosis and inflammation. (researchsquare.com)
The results of the present study indicate that microRNAs play important roles in regulating asthma immune responses. (cdc.gov)

In molecular biology miR-181 microRNA precursor is a small non-coding RNA molecule. (wikipedia.org)
I specifically remember finding a new microRNA and realizing how incredible it was to be the first person to see this biological molecule in writing," Chabot says. (sflorg.com)
MicroRNA is a type of RNA, a chemical cousin of DNA, that attaches to a protein's blueprint (a molecule called messenger RNA) and blocks the production of proteins from it. (medlineplus.gov)

Inhibition of the microRNAs was sufficient to induce EMT in a process requiring upregulation of ZEB1 and/or SIP1. (nih.gov)

MIT post-doc Joel Neilson, left, graduate student Margaret Ebert and Institute Professor Phillip Sharp have developed a new technique to study the function of microRNA that could shed light on the genetic material's hypothesized role in cancer. (mit.edu)
MIT researchers have developed a new way to study the function of microRNA, tiny strands of genetic material that help regulate at least 25 percent of a cell's genes. (mit.edu)
Sequencing of DNA gives a direct picture of our genetic make-up, while sequencing of the RNA that is naturally present in cells gives a picture of which parts of our non-coding DNA are used and in what way. (lu.se)

MicroRNAs, as their name implies, are small RNAs that provide an essential regulation of gene expression by controlling translation of protein expressing messenger RNAs. (rvc.ac.uk)

Loss of miR-31 expression and increased GNA13 expression could be used as biomarkers of breast cancer progression. (biomedcentral.com)

Using cell culture, animal and patient studies, we investigated the biological role of microRNA-96 (miR-96) and microRNA-379 (miR-379) in prostate cancer bone metastasis. (lu.se)

Thus, to maintain a balance between host preservation and inflammatory pathology, the immune response and inflammation need to be tightly regulated. (frontiersin.org)

Research conducted by three independent groups and reported in The Plant Cell shows that fundamental developmental processes controlled by the plant hormone auxin are regulated by microRNAs in the model plant Arabidopsis thaliana. (sciencedaily.com)
Auxin influences development by affecting the expression of numerous genes that control the processes of cell division and cell expansion in specific plant tissues at specific stages during the plant life cycle - e.g. for leaves, roots, and floral organs to develop in the correct patterns and correct time sequence. (sciencedaily.com)
Nanoscopic astrocytic processes contacting synapses and blood vessels contain the molecular machinery for microRNAs. (dcu.ie)
We employ super-resolution microscopy to visualise astrocyte processes and microRNAs in rodent and human brain. (dcu.ie)
If these microRNAs are over or under expressed (increase or decrease in quantity) - for example owing to epigenetic changes to the DNA - this will have an impact on many other regulatory processes. (lu.se)
We hope to be able to contribute to the development of a whole new type of drug that, because of its resemblance to the cell's own RNA, can effectively influence cell processes that have not previously been exploited in treatment. (lu.se)

p class=\'abstract\'>Lin28/LIN-28 is a conserved RNA-binding protein that promotes proliferation and pluripotency and can be oncogenic in mammals. (stanford.edu)

MicroRNAs participate in the regulation of asthma, the goal of this study is to summarize recent researches on the roles of microRNAs in the pathogenesis of asthma. (cdc.gov)

The genes of two octopus species show an increase in microRNAs-which are linked to the development of advanced cells with specific functions-over evolutionary time that has so far only been found in humans, mammals, and other vertebrates. (sflorg.com)

Given the significance of microRNA-mediated gene expression regulation, we are interested in understanding how microRNA and mRNA interactions take place and how these interactions may be overridden by regulated alterations of 3' UTR sizes. (metu.edu.tr)

The expression of miR-181c is relevant to characterize a Breast cancer form, the HER2/neu. (wikipedia.org)
Consistent with their role in regulating EMT, expression of these microRNAs was found to be lost in invasive breast cancer cell lines with mesenchymal phenotype. (nih.gov)
Expression of the miR-200 family was also lost in regions of metaplastic breast cancer specimens lacking E-cadherin. (nih.gov)
This project was carried out to determine (i) whether enhanced GNA13 expression is important for breast cancer cell invasion, and (ii) if so, the mechanism of deregulation of GNA13 expression in breast cancers. (biomedcentral.com)
Additionally our data shows that miR-31 regulates breast cancer cell invasion partially via targeting GNA13 expression in breast cancer cells. (biomedcentral.com)
A recent study of the disparity in breast cancer mortality between AA and EA women concluded that differences in mortality are driven by higher hazard rates of breast cancer death in AA women, irrespective of ER expression [9]. (cdc.gov)
There is currently no gene expression assay that can assess if premalignant lesions will develop into invasive breast cancer. (cdc.gov)

Our aim was to investigate these redox transcription factors in conjunction with redox-related microRNAs in naevi and melanoma. (hindawi.com)

Gene expression programmes drive many of the changes underlying network reorganisation in epileptogenesis, including neurodegeneration, astrogliosis, microgliosis, aberrant neurogenesis and restructured local and distant neuronal contacts, among other changes [ 3-5 ]. (researchsquare.com)

There are many AGO1-like proteins in animals and other eukaryotes as well, indicating that the RNA-induced silencing complex is of ancient evolutionary origin, and that microRNA-mediated regulation of gene expression is shared among many eukaryotes. (sciencedaily.com)

One of these targets is the transcription factor AUXIN RESPONSE FACTOR17 (ARF17), which is thought to repress the expression of a number of other genes involved in auxin responses. (sciencedaily.com)
The protein expression of one of the predicted common targets of miR‑497, insulin‑like growth factor‑1 receptor (IGF‑1R), was assessed using western blot analyses and immunohistochemistry. (spandidos-publications.com)
Over-expression of miR-22 has been reported to protect in models of brain injury and additional targets of miR-22 have been identified [ 17 , 18 ]. (researchsquare.com)
Furthermore, microRNAs are also shown to be targets for asthma therapy in the future. (cdc.gov)

MicroRNA binds to messenger RNA (mRNA), preventing it from delivering protein assembly instructions, thereby inhibiting gene expression. (mit.edu)
When a microRNA is present in the cell, it binds to the target sites and downregulates GFP expression, while in cells that do not express the microRNA GFP, it is expressed. (lu.se)

We found that all five members of the microRNA-200 family (miR-200a, miR-200b, miR-200c, miR-141 and miR-429) and miR-205 were markedly downregulated in cells that had undergone EMT in response to transforming growth factor (TGF)-beta or to ectopic expression of the protein tyrosine phosphatase Pez. (nih.gov)
Conversely, ectopic expression of these microRNAs in mesenchymal cells initiated mesenchymal to epithelial transition (MET). (nih.gov)
Ectopic expression of miR-31 in MDA-MB-231 cells significantly reduced GNA13 mRNA and protein levels, as well as GNA13-3′-UTR-reporter activity. (biomedcentral.com)
Further, expression of miR-31 significantly inhibited MDA-MB-231 cell invasion, and this effect was partly rescued by ectopic expression of GNA13 in these cells. (biomedcentral.com)

In this work, RVC researchers set about to understand microRNA regulation of helper T cells development. (rvc.ac.uk)
Previously it was known that microRNAs were important for the development of a subset of helper T cells called regulatory T cells, which are required to regulate the immune response and prevent attacks against host tissue that lead to autoimmunity. (rvc.ac.uk)
MicroRNA-15b/16 Enhances the Induction of Regulatory T Cells by Regulating the Expression of Rictor and mTOR. (rvc.ac.uk)
The role of miR‑497 in regulating the proliferation of HCC‑derived cells was also investigated in vitro and in vivo. (spandidos-publications.com)
In EBV latently infected epithelial cells, such as nasopharyngeal carcinoma (NPC) and gastric carcinoma (GaCa) cells, viral protein expression is low. (duke.edu)
The level of miR-128-3p was decreased, and TGFBR2 expression was increased in serum samples of sepsis patients and LPS-induced HK2 cells. (degruyter.com)
Macrophages are essential inflammatory cells which regulate the features of immune reactions within tumors. (cancerindex.org)
Until recently, T-cells were divided into well-defined subsets, identified by the expression of a key cytokine. (dcu.ie)
That could change, however, now that MIT Institute Professor Phillip Sharp and his colleagues have found a way to inhibit the activity of microRNA by genetically altering cells. (mit.edu)
The MIT team has found a way to block microRNA activity by tricking cells into producing a microRNA "sponge," which soaks up microRNA and renders it ineffective. (mit.edu)
Sharp and MIT biology graduate student Margaret Ebert, lead author of the paper, decided to block microRNA activity by creating a gene that produces microRNA sponges and inserting it into their target cells. (mit.edu)
More importantly, the sponge technique could be used to create transgenic animals that express the sponge in all of their cells, allowing researchers to study microRNA function at the organismal level. (mit.edu)
Overexpression of GNA13 in MCF-10a cells induced invasion, whereas knockdown of GNA13 expression in MDA-MB-231 cells inhibited invasion. (biomedcentral.com)
Conversely, blocking miR-31 activity in MCF-10a cells induced GNA13 mRNA, protein and 3′-UTR reporter activity. (biomedcentral.com)
Appropriate regulation of autophagy is crucial for clearing toxic proteins from cells. (elifesciences.org)
Here, we report that the microRNA miR-1 regulates the autophagy pathway through conserved targeting of the orthologous T re-2/ B ub2/ C DC16 (TBC) Rab GTPase-activating proteins TBC-7 and TBC1D15 in Caenorhabditis elegans and mammalian cells, respectively. (elifesciences.org)
Further, we found that the cytokine interferon-β (IFN-β) can induce miR-1 expression in mammalian cells, reducing TBC1D15 levels, and safeguarding against proteotoxic challenges. (elifesciences.org)

These data suggest that downregulation of the microRNAs may be an important step in tumour progression. (nih.gov)
C. Elegans LIN-28 Controls Temporal Cell Fate Progression by Regulating LIN-46 Expression via the 5' UTR of Lin-46 MRNA. (stanford.edu)
pubMedId':'34496246','pubMedUrl':'https://www.ncbi.nlm.nih.gov/pubmed/34496246','publicationId':'826388','title':'C. elegans LIN-28 controls temporal cell fate progression by regulating LIN-46 expression via the 5' UTR of lin-46 mRNA. (stanford.edu)

As a consequence, both factors are transported to the nucleus to induce the expression of their target genes. (hindawi.com)

They regulate protein expression by binding to and suppressing translation of messenger RNAs. (rupress.org)

MicroRNA-30c expression was suppressed by hypoxia-inducible factor-1α activation and related to decreased mTOR activity as well as glycolysis in human GC TAMs. (cancerindex.org)

The plant hormone indole-3-acetic acid (IAA), commonly referred to as auxin, plays a major role in regulating plant growth and development. (sciencedaily.com)
Although glucocorticoid receptors (GRs) in the hippocampus play a vital role in the regulation of physiological and behavioural responses to stress, the regulation of receptor expression remains unclear. (karger.com)
One of the biggest changes in evolutionary theory in the late 20th century was the growing appreciation for the central role of changes in gene expression in macroevolution. (freethoughtblogs.com)
We also studied the role of A-to-I RNA editing in regulating miR-379 function. (lu.se)
We are interested in microRNAs, which we think have a role in the development of cancer", says Sofi Elmroth. (lu.se)

However, whether miR-497 regulates other target genes in HCC is unknown. (spandidos-publications.com)
Next, I thoroughly demonstrated that the 10 candidate target genes were substantially suppressed by expression of the relevant miR-BARTs, as measured by 3'UTR-containing firefly luciferase (FLuc) expression, mRNA and protein levels, and knockdown of seven of the 10 candidate genes could suppress apoptosis, mimicking the effects of relevant miR-BARTs. (duke.edu)
In Paper I, we showed that miR-96 can upregulate the mRNA and protein expression of adhesion proteins E-Cadherin and EpCAM through direct interaction with target sites in the mRNA coding sequence. (lu.se)
Bioinformatics predicted SCARB2 was the target gene of miR-10 b,and its protein expression was detected. (cnki.net)
Hence, deregulated microRNAs can potentially alter the expression of numerous target proteins. (metu.edu.tr)

Hypoxia decreases macrophage glycolysis and M1 percentage by targeting microRNA-30c and mTOR in human gastric cancer. (cancerindex.org)

MicroRNA function is disrupted in epilepsy and targeting microRNAs in neurons alleviates seizures. (dcu.ie)

RNA sequencing analysis of the hippocampus during the period of epileptogenesis revealed a specific suppression of inflammatory signalling in the hippocampus of miR-22-deficient mice. (researchsquare.com)
Researchers are finding new RNA all the time because sequencing is now so quick and cheap", says Sofi Elmroth, professor at the Division of Biochemistry and Structural Biology at Lund University. (lu.se)
Sequencing is a method used to decide the exact order of the building blocks of DNA or RNA. (lu.se)

Dr. Bonnie Bartel at Rice University in Houston, TX together with Drs. David Bartel and Allison Mallory at the Massachusetts Institute of Technology and the Whitehead Institute for Biomedical Research in Cambridge, MA report experiments using transgenic Arabidopsis plants that produce a version of ARF17 mRNA that resists microRNA-mediated cleavage. (sciencedaily.com)

Upregulated miR-21 suppresses LPS-induced inflammatory cytokine expression by targeting IL-1 receptor-associated kinase 4 (IRAK4), thereby avoiding excessive inflammatory responses. (frontiersin.org)
Furthermore, we demonstrated that miR-21 regulates inflammatory responses through NF-κB signaling pathways. (frontiersin.org)
A significant decrease in microRNA (miR)-30c and an increase in regulated in development and DNA damage responses 1 (REDD1) were detected in human GC TAMs, the transcription of miR-30c was negatively correlated with REDD1. (cancerindex.org)

Therefore, through this work the researchers have identified a novel way of regulating helper T cell development and controlling the immune response. (rvc.ac.uk)
By de-activating microRNA, researchers can observe the resulting effects and determine which genes the microRNA is targeting. (mit.edu)
The sponge gene also includes a "reporter" gene that causes the cell to become fluorescent if it has taken up the gene, so the researchers can know for sure whether the microRNA sponge is being produced in a particular cell. (mit.edu)

Peterson's research has shown that creatures such as placental mammals whose genes have increased in number and complexity over evolutionary time also exhibit increasing concentrations of microRNAs. (sflorg.com)

Therefore, the present study investigated miR‑497 expression in HCC samples and HCC‑derived cell lines using reverse transcription‑quantitative polymerase chain reaction. (spandidos-publications.com)
The expression levels of miR-128-3p and transforming growth factor beta receptors II (TGFBR2) were detected by quantitative polymerase chain reaction (qPCR). (degruyter.com)

Anatomy3

Organisms1

Chemicals and Drugs24

Analytical, Diagnostic and Therapeutic Techniques and Equipment7

Phenomena and Processes17

Disciplines and Occupations1

Information Science3

MiRNAs41

MiRNA18

Nucleotides2

Molecules14

MRNAs5

Tumor8

Endogenous1

Signaling pathway3

Degradation3

Specific microRNAs2

Transcriptional5

Genes they regulate1

Mechanisms5

Differentiation4

Important roles2

Molecule3

Inhibition1

Genetic3

Small1

Biomarkers1

Metastasis1

Inflammation1

Processes6

Abstract1

Roles1

Humans1

Gene Expression1

Breast cancer7

Transcription factors1

Neuronal1

Regulation of gene ex1

Targets4

Binds2

Ectopic expression4

Cells17

Progression3

Induce1

Messenger1

Inducible1

Role5

Target5

MTOR1

Alleviates1

Sequencing3

Cleavage1

Responses3

Researchers3

Evolutionary1

Quantitative polymera2