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  • target protein
  • However I do get some reactivity on a western, show that there is some of my target protein made. (bio.net)
  • To improve host viability and consequently improve the potential for target protein over-expression, well-regulated expression systems should be employed. (neb.com)
  • Lemo21(DE3) allows researchers to sample a wide range of expression levels to find the optimal conditions for each unique target protein. (neb.com)
  • A drawback to this approach is that the released target protein must be purified from the liberated tag and the protease using additional chromatography steps. (neb.com)
  • Additionally, proteases can occasionally cleave off-site which can be detrimental to the target protein. (neb.com)
  • Used in conjunction with a strong-binding affinity tag, an intein can permit one-step purification of a target protein without the dangers of off-site protease cleavage. (neb.com)
  • Additionally, an intein can be used in a manner that leaves no non-native amino acids on the target protein following its excision. (neb.com)
  • The target protein can be specifically released from the fusion protein through induction of intein cleavage by the addition of a thiol-containing buffer (e.g. (neb.com)
  • The target protein then elutes from the column while the CBD-intein portion remains bound. (neb.com)
  • Different IMPACT vectors permit the intein-CBD tag to be added to the N- or C-terminus of a target protein. (neb.com)
  • A target protein expressed with a C-terminal intein-CBD tag retains a thioester group on its C-terminus after intein cleavage. (neb.com)
  • strains
  • The Competent Cells brochure provides information on the different competent cell strains for cloning and protein expression available from NEB. (neb.com)
  • In standard T7 expression strains (e.g. (neb.com)
  • Group B streptococcal Ibc protein antigen: Distribution of two determinants in wild-type strains of common serotypes. (springer.com)
  • Vectors
  • Gateway technology allows highly efficient transfer of the gene of interest into any of a number of expression vectors from a single entry clone. (thermofisher.com)
  • toxic
  • Under all condition the proteins seem to be toxic (decrease in OD), and I cannot detect anything of the right size after fractionation and column purifcation or just fractionation western blotting, antibody detection. (bio.net)
  • Screening
  • GenScript provides effective solutions to your vaccine needs, such as screening and optimizing parameters involved in regulating vaccinal gene expression and immunogenicity. (genscript.com)
  • synthesis
  • As one of the world's leading suppliers of gene synthesis services, GenScript has a proven record of dedication to innovation in our constant efforts to integrate new and updated technology into our gene expression repertoire. (genscript.com)