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Deuterium Exchange MeasurementPulmonary Gas ExchangeExchange Transfusion, Whole BloodSister Chromatid ExchangeHydrogenSodium-Hydrogen AntiporterKineticsRho Guanine Nucleotide Exchange FactorsChromatography, Ion ExchangeSodiumDeuteriumHydrogen-Ion ConcentrationIon Exchange ResinsSodium-Calcium ExchangerMolecular Sequence DataAnion Exchange ResinsAmilorideAmino Acid SequenceMagnetic Resonance SpectroscopyGuanosine DiphosphateBicarbonatesAntiportersProtonsChloride-Bicarbonate AntiportersProtein BindingCation Exchange ResinsChloridesCarrier ProteinsModels, MolecularCarbon DioxideCalciumHealth Insurance ExchangesRecombination, GeneticOxygen IsotopesNeedle-Exchange ProgramsBiological TransportProtein ConformationModels, BiologicalGuanosine TriphosphateProtein Structure, TertiaryWaterAdenosine TriphosphateInternational Educational ExchangeRec A RecombinasesMutation4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidTemperature4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic AcidBinding SitesOxygenBiological Transport, ActiveEscherichia coliCrossing Over, GeneticMass Spectrometryras-GRF1PotassiumProto-Oncogene Proteins c-vavBase SequenceTime FactorsCell MembraneNuclear Magnetic Resonance, BiomolecularProteinsGasesThermodynamicsRabbitsMathematicsVentilation-Perfusion RatioCattleCatalysisProtein Structure, SecondaryAnion Exchange Protein 1, ErythrocyteIon TransportRespiratory TransportAmidesMagnesiumPhosphatesCell LineRecombinant Proteinscdc42 GTP-Binding ProteinGuanine NucleotidesSequence Homology, Amino AcidModels, ChemicalOuabainOxygen ConsumptionAdenosine DiphosphateErythrocytesADP-Ribosylation Factorsrap1 GTP-Binding ProteinsBacterial ProteinsGTP-Binding ProteinsSarcolemmaLithiumGTPase-Activating ProteinsAnionsDiffusionDNArac1 GTP-Binding ProteinSaccharomyces cerevisiaeCells, CulturedMyocardium
PurificationChromatographyRegulatory proteinsConcentrationInsolublePeptideIntact proteinsAntibodiesMRNAMass spectrometryFamily of proteinsGenesConstituent proteinsAnion-exchangeCharacterizationSerum proteinsLong proteinsSeparate proteinsMembrane proteinsPost-translationalProteomicsLipidsBiologicalCation-exchangeFoldMembranesMoleculesInteractionsAbundanceMutationsStack ExchangeCarbsGuanine nucleotide exchangeGTPasesCaloriesSubunitsHumansPhospholipidMixturesKineticsCharacterizeMolecular weightSugarsCytoskeletonFunctionalBacterialExperimentalPathwaysNovel therapeuticTypicallyAntibodyAminoBegingroupDockBiologyLigandOccurLargeExtractionInteraction
Purification6
  • Here we report the expression, purification and biochemical characterization of C9orf72 protein. (bath.ac.uk)
  • To prevent the binding of host cell proteins with exposed histidines, it is essential to include imidazole at a low concentration in the sample and binding buffer ( Optimizing purification of histidine-tagged proteins ). (sigmaaldrich.com)
  • This protocol describes the purification of insoluble or moderately soluble proteins using an electro-separation system for elution from SDS-polyacrylamide gel and the use of the purified proteins for the production of polyclonal antibodies. (researchsquare.com)
  • The latest manual in the Basic Methods series contains a collection of convenient and easy to use protein purification protocols along with a sampling of dependable methods for assessing protein-protein interactions. (cshlpress.com)
  • The collection of essential methods found in Basic Methods in Protein Purification and Analysis is mainly drawn from the popular manuals Proteins and Proteomics, Purifying Proteins for Proteomics, and Protein-Protein Interactions, 2nd Ed. In addition to protocols for purification using gel electrophoresis and column chromatography, this book contains tested methods for preparing cellular and subcellular extracts-a critical and often neglected step in successful protein purification. (cshlpress.com)
  • The Wadsworth Center Protein Expression Core is available to help generate the protein of interest and can assist in many aspects of protein expression and/or purification. (wadsworth.org)
Chromatography4
  • Protein and peptide chromatography prior to MS analysis can partly address this issue. (hindawi.com)
  • In the first dimension, ion-exchange chromatography (IEC) separates peptides based on ionic interactions with the solid phase and an increasing salt buffer. (hindawi.com)
  • The chromatography medium provides very high binding capacity for histidine-tagged proteins and shows negligible leakage of Ni 2+ ions. (sigmaaldrich.com)
  • Gas chromatography is suitable for the analysis of complex protein samples. (pharmiweb.com)
Regulatory proteins4
  • Receptor-stimulated guanine-nucleotide-triphosphate binding to guanine-nucleotide-binding regulatory proteins. (eurekamag.com)
  • The data thus provide evidence for an essential difference between GTP[S] and p[NH]ppG binding to guanine-nucleotide-binding regulatory proteins and suggest that, in addition to the nucleotide-exchange reaction, a (thio)phosphate-group-transfer process via guanine-nucleotide-binding regulatory protein beta subunits is involved in the receptor-stimulated binding of guanine nucleotide triphosphates to guanine-nucleotide-binding regulatory proteins. (eurekamag.com)
  • The issue of abundant (often housekeeping) proteins masking regulatory proteins of lower abundance (such as signaling proteins and cytokines) continues to be a challenging issue for proteomics particularly in the case of biofluids. (hindawi.com)
  • RGA and GAI belong to the DELLA subfamily within the GRAS family of plant regulatory proteins. (nih.gov)
Concentration9
  • This allows us to calculate the concentration of protein capable of binding to the drug. (stackexchange.com)
  • 5 A further increase of detergent concentration causes progressive delipidation of the lipid-protein-detergent mixed micelles. (sigmaaldrich.com)
  • Plasma has a significant dynamic range, with more than 10 orders of magnitude separating albumin concentration and the rarest measurable proteins identified to date [ 10 ]. (hindawi.com)
  • The optimal concentration of imidazole needed in the sample and buffer to obtain the best purity and yield differs from protein to protein. (sigmaaldrich.com)
  • Add the suitable inducer for the expression of the protein of interest according to the plasmid vector used [e.g., 0.5 mM (final concentration) IPTG ] and incubate for an additional 2 h. (researchsquare.com)
  • Load an aliquot of elution product on mini SDS-polyacrylamide gel along with bovine serum albumin (BSA) of known concentration to evaluate quantity and purity of the purified protein ( Fig. 3 ). (researchsquare.com)
  • If necessary, purified proteins may be concentrated to the required concentration using suitable centrifugal filters (e.g. (researchsquare.com)
  • Can detect specific amino acids based on unique absorbance spectra, thus providing valuable information about the concentration of amino acids in protein samples. (pharmiweb.com)
  • Rounding out the manual are methods for characterizing protein-protein interactions, an extensive appendix of essential methods for quantifying protein concentration, stabilizing and storing proteins, concentrating proteins, and immunoblotting. (cshlpress.com)
Insoluble1
  • Silks are composed principally of proteins with a predominance of alanine, serine and glycine and silk proteins are able to undergo irreversible transformations from soluble protein to insoluble fibres. (bioone.org)
Peptide7
  • The thiophosphorylation state of this protein was regulated by guanine nucleotides, Mg2+ and, most importantly, by activated formyl-peptide receptors. (eurekamag.com)
  • Following activation by agonists such as thrombin, platelets release storage granules and membrane vesicles that contain prothrombotic (e.g., fibrinogen), mitogenic (e.g., platelet derived growth factor), immunomodulatory (e.g., neutrophil-activating peptide 2), and adhesive (e.g., platelet endothelial cell adhesion molecule) proteins. (hindawi.com)
  • The hydrolysis process breaks the peptide bonds, converting proteins into their constituent amino acids. (pharmiweb.com)
  • This pD dependence is due to labile protein deuterons in acidic side-chains and surface peptide groups, which exchange rapidly with water deuterons. (lu.se)
  • All recognized mutations for AD are associated with increased deposition of amyloid-beta (Abeta), a peptide fragment comprising 39-43 amino acids that derive from the catabolism of the amyloid precursor protein (APP) molecule. (medscape.com)
  • We describe a statistical measure, Mass Distance Fingerprint, for automatic de novo detection of predominant peptide mass distances, i.e., putative protein mod- ifications. (lu.se)
  • The need to keep track of protein modifications is readily recognized by the proteomics community, and few repositories of known peptide modifications have been created. (lu.se)
Intact proteins1
  • We used consecutive orthogonal separation platforms to ensure sensitive detection: (a) ion-exchange of intact proteins, (b) SDS-PAGE separation of ion-exchange fractions and (c) HPLC separation of tryptic digests coupled to electrospray tandem mass spectrometry. (hindawi.com)
Antibodies8
  • Abcam: antibodies, proteins, kits. (abcam.com)
  • HX-MS can be used to study a wide range of structural properties including protein folding, ligand binding and allostery, and epitope mapping in the development of therapeutic antibodies. (diwou.com)
  • He had to make his own FITC, label his own antibodies, pour his own column, then build his own fluorescence detector for their HPLC using an automobile halogen headlight bulb and then run hundreds of HPLCs at different protein concentrations and salt concentrations and pH. (stackexchange.com)
  • Like any other protein, antibodies will aspecifically bind nitrocellulose or PVDF membranes, but any other protein present in your antibody preparation will also do. (stackexchange.com)
  • Finally, purified proteins in ammonium bicarbonate buffer or lyophilized may be inoculated in rabbits using standard protocols to produce polyclonal antibodies. (researchsquare.com)
  • B cells produce proteins called antibodies, which attach to foreign particles and germs and mark them for destruction. (medlineplus.gov)
  • The DOCK8 protein is also involved in chemical signaling pathways that stimulate B cells to mature and produce antibodies. (medlineplus.gov)
  • A lack of DOCK8 protein also impairs B cell maturation and the production of certain antibodies. (medlineplus.gov)
MRNA2
  • I wish to find an order of magnitude for the binding/unbinding constants of mRNA and RNA-binding proteins. (stackexchange.com)
  • mRNA and protein expression were assessed by qPCR and Western blot, respectively. (rug.nl)
Mass spectrometry4
  • Hydrogen/deuterium exchange coupled to mass spectrometry (HX-MS) is a valuable technique for analyzing the structural features and dynamic properties of individual proteins and large protein complexes. (diwou.com)
  • The post Automated Protein Structure Analysis Using DIA Based Hydrogen Deuterium Exchange Mass Spectrometry appeared first on GEN - Genetic Engineering and Biotechnology News . (diwou.com)
  • The integrated system was coupled to mass identity of selected proteins by MALDI-TOF mass spectrometry. (lu.se)
  • A combination of molecular simulation with hydrogendeuterium-exchange mass spectrometry and electron paramagnetic resonance spectroscopy reveal an asymmetry across the membrane: ATP-induced conformational changes in the cytosolic cavity promote unfolded pre-protein structure, while the exterior cavity favours its formation. (elifesciences.org)
Family of proteins2
Genes4
  • A number of bleeding disorders arise as a result of mutations in the genes for proteins involved in platelet aggregation. (hindawi.com)
  • To investigate further the function of the RGL genes, we examined the expression profiles of all 5 DELLA protein genes by real-time PCR. (nih.gov)
  • The reports on base sequences of spider silk protein genes have gained importance as the mechanical properties of silk fibers have been revealed. (bioone.org)
  • For example, an exchange of genes between a human influenza A virus and an avian influenza A virus can create a new influenza A virus with a hemagglutinin protein or both a hemagglutinin protein and a neuraminidase protein from an avian influenza A virus. (cdc.gov)
Constituent proteins2
  • The constituent proteins are normally synthesised in specialised glands where the epithelial cells are responsible for the biosynthesis. (bioone.org)
  • Consequently, a lot of effort is put into identifying the constituent proteins using mass spectrometric methods. (lu.se)
Anion-exchange4
  • Anion exchange protein 3 is a membrane transport protein that in humans is encoded by the SLC4A3 gene. (wikipedia.org)
  • Earlier, reversed-phase, weak and strong anion-exchange and weak cation-exchange monolithic columns were introduced in a 1 mm i.d. format for protein separations. (chromatographyonline.com)
  • This study examines the effect of different salt types on protein retention and selectivity in anion exchange systems. (edu.in)
  • Particularly, linear retention data for various proteins were obtained on two structurally different anion exchange stationary-phase materials in the presence of three salts with different counterions. (edu.in)
Characterization2
  • However, for further physicochemical and biochemical characterization of membrane proteins, it is often necessary to remove the unbound detergent. (sigmaaldrich.com)
  • Proteomic approaches have proven powerful at identifying large numbers of proteins, but there are fewer reports of functional characterization of proteins in biological tissues. (hindawi.com)
Serum proteins3
  • We evaluated serum proteins and blood cell gene expression of biomarkers related to potential cardiovascular effects. (cdc.gov)
  • At 4hr, a marked systemic inflammatory response was evidenced by increased inflammatory serum proteins (e.g. (cdc.gov)
  • At 24hr, inflammatory serum proteins and blood cell gene expression had returned to baseline and the systemic tissue response had diminished. (cdc.gov)
Long proteins1
  • begingroup$ If we're talking about plentiful live organisms, sure, but if we have only DNA fragments in fossils, we may not have complete long proteins to work with. (stackexchange.com)
Separate proteins2
Membrane proteins9
  • How Do Detergents Solubilize Membrane Proteins? (sigmaaldrich.com)
  • Common applications include cell lysis, solubilization of membrane proteins and lipids, protein crystallization, and reduction of background staining in blotting experiments. (sigmaaldrich.com)
  • Even though studying membrane proteins is a major challenge in protein biochemistry, they remain an important area of study due to their significant biological and pharmacological relevance. (sigmaaldrich.com)
  • Understanding the structure and function of membrane proteins requires their careful isolation in the native form in a highly purified state. (sigmaaldrich.com)
  • 1 These integral membrane proteins (IMPs) ( Figure 2 ) are not soluble in aqueous solutions as they aggregate to protect their hydrophobic domains, but are soluble in detergent solutions as micelles formed by detergents are analogous to the bilayers of the biological membranes. (sigmaaldrich.com)
  • Hydrophobic regions of membrane proteins, normally embedded in the membrane lipid bilayer, are now surrounded by a layer of detergent molecules and the hydrophilic regions are exposed to the aqueous medium. (sigmaaldrich.com)
  • This keeps the membrane proteins in solution. (sigmaaldrich.com)
  • Complete removal of detergent could result in aggregation due to the clustering of hydrophobic regions and, hence, may cause precipitation of membrane proteins. (sigmaaldrich.com)
  • Excess detergent is normally employed in solubilization of membrane proteins to ensure complete dissolution of the membrane and provide a large number of single protein molecule containing micelles. (sigmaaldrich.com)
Post-translational3
  • These alterations may arise from cancer-specific changes in gene copy number, as well as from other genetic, epigenetic and post-translational changes resulting in higher protein expression, thereby enhancing tumour progression and metastasis. (nature.com)
  • Protein amino acid analysis is a technique for determining the type and amounts of amino acids in a protein sample, providing information on protein composition, post-translational modifications and overall protein quality. (pharmiweb.com)
  • The method can be used for automated detection of chemical/post- translational modifications, quality control of experiments and labelling approaches, and to control the modification settings of protein identification tools. (lu.se)
Proteomics2
  • While modern proteomics experiments permit the analysis of hundreds to thousands of proteins in complex samples, the most powerful use of this data would combine information on protein activity with the identities of the active proteins. (hindawi.com)
  • The success of proteomics experiments, such as studies of protein function and cell signaling pathways, ultimately de- pends on how well the protein content in samples is identified and annotated. (lu.se)
Lipids1
  • Proteins and lipids are embedded in this bilayer forming the fluid mosaic model ( Figure 2 ) which was first proposed by Singer and Nicolson in 1972. (sigmaaldrich.com)
Biological3
  • Complex biological samples have also been successfully separated in multiple dimensions at the protein level. (hindawi.com)
  • This involves protein extraction from the biological sample of interest, followed by protein denaturation to break down the tertiary structure and expose the amino acids. (pharmiweb.com)
  • Understanding how proteins function is an essential part of many biological research endeavors. (cshlpress.com)
Cation-exchange3
  • A New High-resolution Strong Cation-exchange 1 mm i.d. (chromatographyonline.com)
  • ProSwift strong cation-exchange (SCX) column is a polymethacrylate-based monolithic column with sulfonate functionality. (chromatographyonline.com)
  • The cation-exchange restricted access packings were characterised with respect to their adsorption and desorption kinetics. (lu.se)
Fold2
Membranes2
  • Transport of proteins across membranes is a fundamental process, achieved in every cell by the 'Sec' translocon. (elifesciences.org)
  • The encapsulation and compartmentalisation of cells has necessitated the evolution of machineries that conduct proteins across membranes, including for protein secretion and organellar import. (elifesciences.org)
Molecules9
  • At concentrations equal to, or higher than the detergent's CMC, the lipid bilayer becomes saturated with detergent molecules and breaks apart generating lipid-protein-detergent mixed micelles (c). 8 A detergent/protein ratio of around 1-2 (w/w) is believed to be sufficient to solubilize IMPs to form lipid-protein-detergent mixed micelles. (sigmaaldrich.com)
  • begingroup$ Docking software and server are specialized in small molecules, peptides and proteins. (stackexchange.com)
  • Proteins are large molecules, which occur in abundance in every single living organism. (knoldus.com)
  • How many protein molecules in a single cell? (cosmosmagazine.com)
  • Consider, for instance, this one: How many protein molecules in a single cell? (cosmosmagazine.com)
  • In the end, they discovered that most of the proteins fell into a fairly narrow range, containing between 1000 and 10,000 molecules. (cosmosmagazine.com)
  • Techniques and insight from this study will likely lead to new attempts to quantify protein molecules in human cells - and thus to insights into the mechanisms involved in protein-implicated diseases. (cosmosmagazine.com)
  • 699 guanidinium (Eigen, 1964) groups that exchange and 17O relaxation data may reveal slowly exchanging sufficiently rapidly to contribute to the spin internal water molecules. (lu.se)
  • In the journal Nature, Manuela Raffatellu, associate professor of microbiology & molecular genetics, and colleagues provide the first evidence that small protein molecules called microcins, produced by beneficial gut microbes, play a critical part in blocking certain illness-causing bacteria in inflamed intestines. (infectioncontroltoday.com)
Interactions8
  • Learning how to use HX-MS to study drug binding interactions and higher-order protein structure dynamics. (diwou.com)
  • 2002. Kinetic studies of RNA-protein interactions using surface plasmon resonance. (stackexchange.com)
  • There are many methods for studying kinetics, I only mention this one because the lab I'm in has used it for studying protein-ligand interactions. (stackexchange.com)
  • I really couldn't tell you if those rates are generally representative of RNA-protein interactions, but I suppose that's not what you're asking. (stackexchange.com)
  • Proteins are held in the lipid bilayer by hydrophobic interactions between the lipid tails and hydrophobic protein domains. (sigmaaldrich.com)
  • 2 Proteins are incorporated into these micelles via hydrophobic interactions. (sigmaaldrich.com)
  • Maybe you can use a different method to study the interactions between the proteins and glycans (ex. (stackexchange.com)
  • If this software aren't able to treat your system, you can use a different method to study the interactions between the proteins and glycans, ex. (stackexchange.com)
Abundance3
  • What they do contain in abundance is calories, all protein nothing else. (stackexchange.com)
  • To make the finding, a team led by Grant Brown from the University of Toronto in Canada combined the data from just under two dozen large studies looking at protein abundance in yeast cells. (cosmosmagazine.com)
  • Protein abundance has been studied in many different contexts. (cosmosmagazine.com)
Mutations2
  • Recent cancer genome deep sequencing efforts have revealed an unanticipated high frequency of mutations in G proteins and G-protein-coupled receptors (GPCRs) in most tumour types. (nature.com)
  • These deletions and other DOCK8 gene mutations lead to production of an abnormally short protein or production of no protein. (medlineplus.gov)
Stack Exchange11
  • Stack Exchange network consists of 183 Q&A communities including Stack Overflow , the largest, most trusted online community for developers to learn, share their knowledge, and build their careers. (stackexchange.com)
  • Physical Fitness Stack Exchange is a question and answer site for physical fitness professionals, athletes, trainers, and those providing health-related needs. (stackexchange.com)
  • Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. (stackexchange.com)
  • Bioinformatics Stack Exchange is a question and answer site for researchers, developers, students, teachers, and end users interested in bioinformatics. (stackexchange.com)
  • Thanks for contributing an answer to Bioinformatics Stack Exchange! (stackexchange.com)
  • Chemistry Stack Exchange is a question and answer site for scientists, academics, teachers, and students in the field of chemistry. (stackexchange.com)
  • Medical Sciences Stack Exchange is a question and answer site for professionals in medical and allied health fields, students of those professions, related academics, and others with a sound understanding of medicine and healthcare-related sciences. (stackexchange.com)
  • Worldbuilding Stack Exchange is a question and answer site for writers/artists using science, geography and culture to construct imaginary worlds and settings. (stackexchange.com)
  • Matter Modeling Stack Exchange is a question and answer site for materials modelers and data scientists. (stackexchange.com)
  • By clicking "Accept all cookies", you agree Stack Exchange can store cookies on your device and disclose information in accordance with our Cookie Policy . (stackexchange.com)
  • Space Exploration Stack Exchange is a question and answer site for spacecraft operators, scientists, engineers, and enthusiasts. (stackexchange.com)
Carbs2
  • Do you know where I can find a list of things that have calories but aren't carbs, proteins, or fat? (stackexchange.com)
  • The ratio of protein to carbs and fats has some effect, but it's less than total calories. (stackexchange.com)
Guanine nucleotide exchange5
  • The C9orf72 protein is predicted to be a differentially expressed in normal and neoplastic cells domain protein implying that C9orf72 functions as a guanine nucleotide exchange factor (GEF) to regulate specific Rab GTPases. (bath.ac.uk)
  • Iyer, S , Subramanian, V & Acharya, KR 2018, ' C9orf72, a protein associated with amyotrophic lateral sclerosis (ALS) is a guanine nucleotide exchange factor ', PeerJ , pp. 1-29. (bath.ac.uk)
  • The interaction of Ran with the guanine nucleotide exchange factor protein RCC1 was also studied by (31)P NMR spectroscopy. (mpg.de)
  • In the presence of nucleotide, the ternary complex of Ran.nucleotide.RCC1, an intermediate in the guanine nucleotide exchange reaction, could be observed. (mpg.de)
  • The proteins in this family act as guanine nucleotide exchange factors (GEFs). (medlineplus.gov)
GTPases1
  • GEFs turn on (activate) proteins called GTPases, which play an important role in chemical signaling within cells. (medlineplus.gov)
Calories4
  • As an addendum to this, protein shakes are good when you are on a diet and need to keep the protein up and the calories down. (stackexchange.com)
  • If you need to keep your Calories down and still meet the protein requirements, then a whey protein supplement is definitely a good way to do that. (stackexchange.com)
  • If you've taken your regular diet and added in 165 calories of protein on top of it, you're almost certainly going to gain weight. (stackexchange.com)
  • The Nutricia Pro-Stat® Sugar Free High Protein Nutritional Drink is a sugar-free, ready-to-drink medical food providing 15 grams of enzyme-hydrolyzed complete protein and 100 calories per 1 fl oz. (senior.com)
Subunits2
  • Glycerol density gradient centrifugation and dynamic light scattering studies show that the multimeric size of the mutant proteins is significantly reduced, indicating a role for this motif in higher order organization of the subunits. (nih.gov)
  • Fluorescence resonance energy transfer studies show that the rate of exchange of the subunits in the alpha Adel-crystallin oligomer is higher compared with that in the alpha A-crystallin oligomer, suggesting that this region contributes to the oligomer dynamics in addition to the higher order assembly and structural stability. (nih.gov)
Humans1
  • Theoretically, any combination of the 18 hemagglutinins and 11 neuraminidase proteins are possible, but not all have been found in animals and even fewer have been found to infect humans. (cdc.gov)
Phospholipid1
  • Pathogenesis and sporulation are complex processes that are governed by cellular signalling networks including G-protein and phospholipid signalling. (wur.nl)
Mixtures1
  • The ProSwift SCX-1S, 1 mm i.d. column provides high-resolution and cost-effective separation of simple and complex protein mixtures. (chromatographyonline.com)
Kinetics1
Characterize1
Molecular weight1
  • The IERAM material was also investigated by capacity, selectivity, and biocompatibility determinations when applied to the quantification of small molecular weight proteins such as cytochrome C, Lysozyme, Ribonuclease A, Myoglobin, Insulin, human serum albumin, and a Tryptic inhibitor. (lu.se)
Sugars1
Cytoskeleton3
  • The distinctive presence of both Rab- and Rho-GTPase GEF activities suggests that C9orf72 may function as a dual exchange factor coupling physiological functions such as cytoskeleton modulation and autophagy with endocytosis. (bath.ac.uk)
  • Signaling stimulated by DOCK family proteins are typically involved in the arrangement of the structural framework inside cells (the cytoskeleton). (medlineplus.gov)
  • By controlling the shape of the cytoskeleton, DOCK family proteins play a role in cell structure and movement (migration). (medlineplus.gov)
Functional2
  • As a result, affected individuals have little or no functional DOCK8 protein. (medlineplus.gov)
  • These approaches promise to enable researchers to assess, on a large scale, both expression level and functional state of the proteins that carry out most functions in a cell. (lu.se)
Bacterial1
  • The proteins to be purified must be highly expressed in bacterial cells and visible in polyacrylamide gels following Coomassie Blue staining. (researchsquare.com)
Experimental4
  • Quantitative structure retention relationship (QSRR) models based on support vector machine feature selection and regression models were developed using the experimental chromatographic data in conjunction with various molecular descriptors computed from protein crystal structure geometries. (edu.in)
  • Here, we describe an experimental approach that fractionates proteins released from human platelets, linking bioassay activity to identity. (hindawi.com)
  • This blog post is about a software package I wrote, MR-REX, which is part of a pipeline to determine protein structures using experimental data. (knoldus.com)
  • Unfortunately, there is no way to calculate the structure a protein, from the intensities of the scattered X-ray light, because there is needed information missing from the experimental data. (knoldus.com)
Pathways2
  • These findings suggest that these protein family members may function at the same site during vesicular transport rather than in separate pathways. (nih.gov)
  • However, it is unknown whether cAMP protects against DILI and, if so, which downstream targets of cAMP are implicated in the protective mechanism including the classical protein kinase A (PKA) pathway or alternative pathways like the exchange protein directly activated by cAMP (EPAC). (rug.nl)
Novel therapeutic3
  • G proteins, GPCRs and their linked signalling circuitry represent novel therapeutic targets for cancer prevention and treatment. (nature.com)
  • These studies indicate that G proteins, GPCRs and their linked signalling circuitry represent novel therapeutic targets for cancer prevention and treatment. (nature.com)
  • Thus, altered or deregulated platelet function underpins many diseases, and platelet proteins are potential targets for novel therapeutic agents. (hindawi.com)
Typically2
  • The RSD data were typically less than 8% at a 50 μM protein level (n = 7). (lu.se)
  • 6 Typically, a detergent/protein ratio of around 10 (w/w) or higher will lead to complete delipidation. (sigmaaldrich.com)
Antibody2
  • Depending on the antibody class, more specific binding can be obtained with protein A or protein G, that recognize the Fc domain. (stackexchange.com)
  • The antibody should be free of other proteins for best results as these will compete for binding (many are supplied with carrier proteins such as BSA that will compete). (stackexchange.com)
Amino11
Begingroup1
  • begingroup$ Immediately after posting this question, ideas are forming about a massive strangler vine that constricts gigantic prey, and then puts a large part of the protein acquired this way into an underground bulb for storage - basically a protein potato. (stackexchange.com)
Dock1
Biology1
  • Knowing the shapes of proteins is critical to understanding biology and curing diseases. (knoldus.com)
Ligand1
  • Previous proteomic studies of intact platelets have collectively identified hundreds of proteins using a variety of fractionation strategies including 2-dimensional electrophoresis (2DE), multidimensional chromatographic separations, membrane prefractionation techniques, and adsorption to combinatorial hexapeptide ligand libraries [ 1 - 6 ]. (hindawi.com)
Occur2
  • So we know for sure that two similar proteins never occur independently anywhere in the visible universe. (stackexchange.com)
  • We showed that RGL2 protein in imbibed seeds is rapidly degraded by GA treatment and that the F-box protein SLY1 is required for RGL2 degradation to occur. (nih.gov)
Large3
  • Do you know of a good software or web server tool that helps doing docking of proteins with rather large glycans? (stackexchange.com)
  • Yeast is the only organism that has been studied often and thoroughly enough to produce sufficient information to permit large scale analysis of the molecular make-up of cell proteins - but that didn't mean the job of Brown's team was simply one of mathematics. (cosmosmagazine.com)
  • All globular proteins contain large numbers of interaction, in particular, its dynamic aspects labile hydrogen atoms that can exchange with the (Daszkiewicz et al . (lu.se)
Extraction1
  • Solid phase extraction coupled on-line with a microbore reversed phase HPLC system for the quantitation of small marker proteins is demonstrated. (lu.se)
Interaction1
  • These proteins may modulate the interaction of platelets with their local cellular environment. (hindawi.com)