Loading...
  • biochemistry
  • Proteins are used as sensors to monitor activity or biochemistry of neurons, to control or block action potential firing of neurons, or to manipulate circuits on longer time scales by pharmaco-genetics. (tu-dresden.de)
  • Using the power of protein engineering and evolution, we can convince enzymes to take what they do poorly and do it really well," says Frances Arnold, the Dick and Barbara Dickinson Professor of Chemical Engineering, Bioengineering and Biochemistry at Caltech and principal investigator on a paper about the enzymes that appears online in Science . (phys.org)
  • First author Stephanie Müller and Prof. Feige in the Laboratory for Cellular Protein Biochemistry. (tum.de)
  • The interleukins are the vocabulary with which immune cells communicate," explains Matthias Feige, Professor of Cellular Protein Biochemistry at the Technical University of Munich. (tum.de)
  • The research was carried out at the Technical University of Munich at the Department of Theoretical Biophysics (Physics Department), in the Laboratory for Cellular Protein Biochemistry (Faculty of Chemistry), the Center for Allergy and Environment (ZAUM) of the TU Munich and the Helmholtz Zentrum Muenchen, as well as at the Université Sorbonne in Paris. (tum.de)
  • Specificity
  • Protein-based tools are at the core of many of strategies to study brain function as they endow a high degree of specificity, allow highlighting designated types of neurons and their subcellular structures, and permit implantation deeply into living brains. (tu-dresden.de)
  • Immunology
  • An interdisciplinary team from the fields of structural biology, immunology and cell biology has now, for the first time, successfully produced a protein that could balance the overshooting immune response. (tum.de)
  • enzyme
  • The enzyme cytochrome P450 is nature's premier oxidation catalyst-a protein that typically promotes reactions that add oxygen atoms to other chemicals. (phys.org)
  • novo
  • Intrexon's proprietary database catalogs comprehensive information about the genetic and protein components based on structure-based sequence alignment, de novo and comparative protein modeling, molecular dynamics simulation and free energy analysis. (dna.com)
  • Thermostability
  • Matthews BW, Nicholson H, Becktel WJ (1987) Enhanced Protein Thermostability from Site-Directed Mutations that Decrease the Entropy of Unfolding. (springer.com)
  • genetic
  • Most genetic mutations that affect the structure of proteins will lead to something that doesn't work, and the death of whatever inherited it. (aawsat.com)
  • In mice and flies, the Arc protein forms capsids and carries genetic information. (the-scientist.com)
  • biological
  • This review chapter attempts to give an accurate representation of the three main strategies employed in the engineering of more suitable biological components for use in biosensor construction. (springer.com)
  • IBPs, including the antifreeze proteins (AFPs) that prevent the freezing of organisms, are found in nearly all biological kingdoms. (queensu.ca)
  • The team then prepared the modified interleukin in the laboratory and tested its biological functions - with very encouraging results: The engineered messenger molecule is recognized by human cells. (tum.de)
  • However, although quinones are common biological redox-active cofactors, we are not aware of any previous work using them as bound electron donor/acceptors in designed or engineered proteins. (pnas.org)
  • Vaccines
  • Some are looking to design new proteins for drugs and vaccines, while others are seeking cleaner catalysts for the chemical industry and new materials. (aawsat.com)
  • strategies
  • One of the strategies for finding an answer to how Alzheimer's disease could be prevented is based on the idea of adding a protein to the blood, to bind to the Aß peptide there. (innovations-report.com)
  • Furthermore with the advent of new nanomaterials the implementation of these design strategies, with the attomolar promise of nanostructures, imparts important generational leaps in research for biosensor construction, based on highly specific, very robust, and electrically wired protein engineered biosensors. (springer.com)
  • Our protein engineering can also be applied to agriculture, including the generation of molecular strategies aimed at developing pest resistance in plants. (dna.com)
  • reveal
  • Millions of measurements from 23 people who consumed extra calories every day for a month reveal changes in proteins, metabolites, and gut microbiota that accompany shifts in body mass. (the-scientist.com)
  • oxygen
  • Proteins work to ferry oxygen through the bloodstream, extract energy from food, fire neurons and attack invaders. (aawsat.com)
  • Quinone binding is stabilized by hydrogen-bonding to the quinone carbonyl oxygen, van der Waal's contact between the protein and quinone head group and especially by hydrophobic binding of the large quinone phytol tail ( 17 ). (pnas.org)
  • stability
  • The use of native proteins for this purpose cannot adequately embrace the limits of detection and level of stability required for a usable sensor, due to globular structure restraints. (springer.com)
  • Consequently, there is a need to find or engineer IBPs with enhanced activity and stability. (queensu.ca)
  • fusion
  • In the first agent, the double stranded RNA binding domain (dsRBD) of human protein kinase R is used as an siRNA carrier, in fusion proteins that target epidermal growth factor receptor (EGFR). (mit.edu)
  • This paper presents a revised assembly strategy that is compatible with the current biobrick definition and permits the construction of fusion proteins. (mit.edu)
  • detection
  • Sotiropoulou S, Fournier D, Chaniotakis NA (2005) Genetically engineered acetylcholinesterase-based biosensor for attomolar detection of dichlorvos. (springer.com)
  • cytochrome
  • A unique quinone-binding site was engineered into the Escherichia coli cytochrome b 562 by introducing a cysteine within the hydrophobic interior of the protein. (pnas.org)
  • volume
  • By convincing many of the key players in the field to contribute to this two volume masterpiece, they managed to assemble basically all aspects of modern protein engineering technology under one roof, that is, between four hard covers. (wiley.com)
  • This report studies the global Protein Engineering market status and forecast, categorizes the global Protein Engineering market size (value & volume) by key players, type, application, and region. (reportsnreports.com)
  • toxic
  • The research team has studied how an artificial so-called Affibody protein entirely encases the Aß peptide, thereby preventing the formation of toxic forms. (innovations-report.com)
  • Another strategy involves using protein engineering to stabilize the toxic oligomers that are the cause of nerve cell death and memory loss. (innovations-report.com)
  • He's also studying Alzheimer's disease and similar neurological conditions, which are characterized by brain proteins that fold up incorrectly into toxic deposits. (aawsat.com)
  • different
  • But certain combinations of different mutations will lead to a modified version of the same thing, allowing new proteins to evolve. (aawsat.com)
  • Expression and processing of proteins in different prokaryotic and eukaryotic cell systems, for innovative hemibodies with defined characteristics. (academics.de)
  • The semiquinone can be generated at high pH by a one-electron reduction, and the midpoint potential of this can be adjusted by ≈500 mV by binding different quinones to the protein. (pnas.org)
  • structure
  • We present a basic protein engineering toolkit consisting of six independent programs, that can be incorporated in a pipeline of various protein structure files. (iitg.ac.in)
  • Nowadays, knowledge of the three-dimensional ( 3D ) structure of a protein is critical in order to gain a full understanding of its function. (cnio.es)
  • power
  • Since then, scientists have advanced their understanding of the physics of proteins, and computing power has increased. (aawsat.com)
  • large
  • The IBP found on the cell surface of a Gram-negative Antarctic bacterium, Marinomonas primoryensis, is one region of an exceptionally large multi-domain 1.5 MDa protein, MpIBP. (queensu.ca)
  • various
  • Various quinones, such as p -benzoquinone and 2,3-dimethoxy-5-methyl-1,4-benzoquinone, were then covalently attached to the protein through a cysteine sulfur addition reaction to the quinone ring. (pnas.org)
  • drugs
  • The race to develop analgesic drugs that inhibit sodium channel Na V 1.7 is revealing a complex sensory role for the protein. (the-scientist.com)
  • complex
  • What's recently changed is the ability to decipher the complex language of protein shapes. (aawsat.com)
  • That won them the ability to predict how smaller proteins would fold, but larger ones were still too complex. (aawsat.com)
  • target
  • According to the study, specific examples of sequence engineering that led to improved expression, duration, and enzymatic activity of target proteins are reported. (siasat.com)
  • Science
  • The research was funded by the German Research Foundation (DFG) in the context of the Collaborative Research Center SFB 1035 and the Cluster of Excellence Center for Integrated Protein Science Munich (CIPSM). (tum.de)
  • system
  • This system, however, is limited in that each protein-encoding biobrick must contain a complete translated region. (mit.edu)
  • It would then seem to be useful to use a protein-based system to mimic or study this photochemistry. (pnas.org)