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  • Antigen
  • An on-nanoparticle rolling-circle amplification (nanoRCA) strategy allows the visual detection of 30 molecules of carcinoembryonic antigen with at least 1010-fold selectivity over a range of noncognate proteins. (ntu.edu.sg)
  • The nanoRCA assay is applicable to the detection of two tumor markers in biological fluids: prostate-specific antigen in spiked sera and interleukin-8 in saliva. (ntu.edu.sg)
  • Labeling
  • Dr. Amedeo Cappione III (Merck) will then outline an alternative method for small-scale protein labeling based upon continuous flow diafiltration in a centrifugal filter. (merckmillipore.com)
  • Our new Click-iT® DIBO reagents now offer a means of achieving click reactions without copper, enabling live-cell surface labeling of proteins and sugars using click chemistry and gentle conjugation of azide-labeled material. (thermofisher.com)
  • The classic click reaction involves copper-catalyzed triazole formation from an azide and an alkyne, but our new DIBO reagents permit the labeling of azide-modified macromolecules without the metal catalysts, which enables live-cell detection and prevents protein damage. (thermofisher.com)
  • multiplex
  • Whether you need ultrasensitive detection from an SMC™ assay, robust ELISAs, rich multiplex profiling data using Luminex® xMAP® technology, or maximum throughput from a Gyrolab® workstation, we help you get data you can trust out of each sample, with minimal repetition, so you can make the best next-step decisions. (merckmillipore.com)
  • localization
  • We help you understand protein structure and function with tools to characterize post-translational modifications, localization and expression control including epigenetic mechanisms. (merckmillipore.com)
  • In its role as a reporter, GFP has added a new dimension to the analysis of protein function and localization, allowing realtime examination in live cells and processes that previously had been observed only through immunocytochemical "snapshots" in fixed specimens. (thermofisher.com)
  • Fluorescent proteins are widely used to investigate gene expression, as well as protein localization, translocation, and trafficking within live cells. (thermofisher.com)
  • membranes
  • In addition, staining does not iterfere with the transfer of proteins to membranes, allowing visualization before preceeding with Western blotting. (sigmaaldrich.com)
  • extracellular
  • c-MPL/TPOR is a 635-amino acid transmembrane protein with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs. (clontech.com)
  • Dentin matrix protein 1 (DMP1) is an acidic extracellular matrix phosphoprotein that can bind calcium. (clontech.com)
  • In mature osteoblasts, the DMP1 protein is phosphorylated and localized to the extracellular matrix, where it plays a role in forming mineralized matrix. (clontech.com)
  • While other constitutively expressed proteins in the extracellular matrix, such as osteopontin and osteocalcin, are expressed in osteoblast cells, DMP1 is expressed in osteocytes, making it a candidate biomarker for osteocyte activity. (clontech.com)
  • intracellular
  • Surface and intracellular proteins are markers that are important for identifying specific type of cells and characterizing cell populations. (nexcelom.com)
  • In the recent years, image-based cytometry method has been utilized for many standard surface and intracellular protein detection assay to provide a more simple and rapid method for cell population analysis. (nexcelom.com)
  • biological
  • Cytokines, hormones, growth factors, and signaling proteins in biological fluids/cells/tissues are critical factors in numerous processes in healthy and disease states, and in most models multiple pathways are involved in simultaneously producing a cellular response to disease or experimental stimulation. (ufl.edu)
  • Both of these technologies enable researchers to more fully understand the complexities of their cell/tissue samples by more accurately quantifying proteins and lipids, saving precious sample volume, and generating dozens of data points from a single biological sample. (ufl.edu)
  • Since the discovery of Green Fluorescent Protein (GFP) in the 1960s, the use of fluorescent proteins (FPs) in biological studies has exploded. (thermofisher.com)
  • Stain
  • If you can't decide which protein gel stain is right for you visit our guide, 'How To Select A Protein Gel Stain' is available in the technical literature. (gbiosciences.com)
  • homolog
  • It can bind to a receptor (the proto-oncogene c-Mpl, a homolog of an envelope protein of the myeloproliferative leukemia virus) on primitive blood cells and megakaryoctes and stimulate those cells to proliferate and evolve into platelet-producing megakaryocytes. (takarabio.com)
  • Staining
  • however, the limitations and experimental disadvantages of absorption-based detection technologies and labor-intensive silver staining techniques have become glaringly apparent. (thermofisher.com)
  • kits
  • Alternatively, the development of multiepitope proteins is an attractive approach to reduce the complexity and the final costs of such diagnostic kits [ 12 ]. (hindawi.com)
  • cell
  • The FBN domain in angiopoietin-like protein 3 (ANGPTL3) interacts with alpha-5 and beta-3 integrins, leading to induction of endothelial cell adhesion and migration. (clontech.com)
  • Galectin proteins contain carbohydrate-recognition-binding domains (CRDs) that bind beta-galactoside sugars and are involved in mediating cell-cell and cell-matrix interactions. (takarabio.com)
  • variants
  • The quest for new FPs with unique excitation and emission profiles has been the focus of many distinguished laboratories, and mutation and evolution of such proteins has led to the creation of variants including the "fruit" FPs (e.g., tdTomato, mStrawberry, mCherry, mPlum, and mRaspberry), specialized FPs such as the fluorescent timer protein Kusabira Green Orange, long-Stokes shift (LSS) fluorescent proteins such as LSS-mKate2, and photoswitchable FPs (e.g. (thermofisher.com)